BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum22_contig00005825
         (1887 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003534478.1| PREDICTED: cysteine protease ATG4-like [Glyc...   523   e-146
ref|XP_003624282.1| Cysteine protease ATG4 [Medicago truncatula]...   518   e-144
ref|XP_002331599.1| predicted protein [Populus trichocarpa] gi|2...   514   e-143
ref|XP_003552483.1| PREDICTED: cysteine protease ATG4-like [Glyc...   513   e-143
ref|XP_002309707.1| predicted protein [Populus trichocarpa] gi|2...   510   e-142

>ref|XP_003534478.1| PREDICTED: cysteine protease ATG4-like [Glycine max]
          Length = 486

 Score =  523 bits (1347), Expect = e-146
 Identities = 275/490 (56%), Positives = 343/490 (70%), Gaps = 17/490 (3%)
 Frame = +2

Query: 284  VMKG--EGIHNLECSFDSPKQLIGS----VSFEAGPSSTKDYKASVWSGFLVQ--SIFDT 439
            V+KG  E I + +CS  S  + + +    V  +AG S++K  KAS+WS       S+ +T
Sbjct: 2    VLKGLCERIVSSKCSSKSSTETVDNTQVPVYSKAGSSNSKFPKASLWSNIFTSGFSVVET 61

Query: 440  CNQSKNYTKKT--SNTNGWTAAVRKVMYSGTMRR----IFGLNRASVSGSRTDIWLLGVC 601
             ++S    KK   S ++GW AAVRKV+  G+MRR    + G +R  +S S  DIWLLGVC
Sbjct: 62   YSESSASEKKAVHSRSSGWAAAVRKVVTGGSMRRFQERVLGSSRTDISSSDGDIWLLGVC 121

Query: 602  YRVAQDDPSADPSQSEGYAAFVEDFSSRICITYRKGFAAIEDTKYTSDAGWGCMLRSSQM 781
            ++++Q + S     S G A+F +DFSS+I +TYRKGF AI DTKYTSD  WGCMLRSSQM
Sbjct: 122  HKISQQESSGGVDNSNGLASFEQDFSSKILVTYRKGFDAIGDTKYTSDVHWGCMLRSSQM 181

Query: 782  LVAQALLVHQLGRSWRKALDKPLEPTYFEILHLFGDSMISAYSIHNLLQAGKAYGLAPGS 961
            LVAQALL H+LGRSWRK +DKP +  Y ++L LFGDS  SA+SIHNLLQAGK YGLA GS
Sbjct: 182  LVAQALLFHKLGRSWRKPIDKPPDKEYIDVLQLFGDSEASAFSIHNLLQAGKGYGLAVGS 241

Query: 962  WVGPYAMCRTWETLSRCMRKESENSGLTSAMSIYVVSGDEDGERGGAPVVCIEDIYNHCV 1141
            WVGPYAMCRTWE L+   RK+++   L   M+IYVVSGDEDGERGGAPVVCIED    C 
Sbjct: 242  WVGPYAMCRTWEVLA---RKKNDLGELPLPMAIYVVSGDEDGERGGAPVVCIEDASKRCF 298

Query: 1142 VSSN--MDWTXXXXXXXXXXXXDKINPRYLPLLGSTFTFPQSLGILGGRPGVSTYIIGVQ 1315
              S+    WT            DK+NPRY+PLL STF FPQSLGI+GG+PG STYIIG Q
Sbjct: 299  EFSSGLAAWTPLLLLVPLVLGLDKVNPRYIPLLRSTFKFPQSLGIMGGKPGASTYIIGAQ 358

Query: 1316 DEKAFYLDPHETQPVVDIMGDCSE-VDTSSFHCSIIRQFPLDSIDPSLAIGFYCRDENDF 1492
            +EKAFYLDPH+ Q VV+I GD  E   TSS+HC+I+R  PLDSIDPSLAIGFYCRD++DF
Sbjct: 359  NEKAFYLDPHDVQQVVNISGDTQEPTSTSSYHCNIMRHIPLDSIDPSLAIGFYCRDKDDF 418

Query: 1493 ADFCSRAIELADQSNGAPLFTITETRKSAKSFSQNDISSDNEMINTDSQFNGVQRGESTR 1672
             DFCS+A +LA++SNGAPLFT+T++R  +K  + ND+S DN     +  F G+ RG  T 
Sbjct: 419  DDFCSQASKLAEESNGAPLFTVTQSRSFSKQVTSNDVSGDNTGFQ-EEDFPGMDRGNDT- 476

Query: 1673 DTQDEDWQFL 1702
             T ++DWQ L
Sbjct: 477  GTNEDDWQLL 486


>ref|XP_003624282.1| Cysteine protease ATG4 [Medicago truncatula]
            gi|147742964|sp|A2Q1V6.1|ATG4_MEDTR RecName:
            Full=Cysteine protease ATG4; AltName:
            Full=Autophagy-related protein 4
            gi|124359485|gb|ABN05923.1| Peptidase C54 [Medicago
            truncatula] gi|355499297|gb|AES80500.1| Cysteine protease
            ATG4 [Medicago truncatula]
          Length = 487

 Score =  518 bits (1334), Expect = e-144
 Identities = 267/477 (55%), Positives = 333/477 (69%), Gaps = 14/477 (2%)
 Frame = +2

Query: 314  ECSFDSPKQLIGSV----SFEAGPSSTKDYKASVWSGFLVQ--SIFDTCNQSKNYTKKT- 472
            +CS  S  +++ +     S +AG S +K  KAS+WS F     S+ +T ++S +  KKT 
Sbjct: 14   KCSSKSSTEIVDNTQVPASSKAGSSDSKFPKASLWSTFFTSGFSVDETYSESSSSEKKTV 73

Query: 473  -SNTNGWTAAVRKVMYSGTMRR----IFGLNRASVSGSRTDIWLLGVCYRVAQDDPSADP 637
             S  +GW AAVRKV+  G+MRR    + G  R  VS S  DIWLLGVC++++Q + + D 
Sbjct: 74   HSRNSGWAAAVRKVVSGGSMRRFQERVLGSCRTDVSSSDGDIWLLGVCHKISQHESTGDV 133

Query: 638  SQSEGYAAFVEDFSSRICITYRKGFAAIEDTKYTSDAGWGCMLRSSQMLVAQALLVHQLG 817
                 +AAF +DF SRI ITYRKGF AIED+KYTSD  WGCMLRSSQMLVAQALL H+LG
Sbjct: 134  DIRNVFAAFEQDFFSRILITYRKGFDAIEDSKYTSDVNWGCMLRSSQMLVAQALLFHKLG 193

Query: 818  RSWRKALDKPLEPTYFEILHLFGDSMISAYSIHNLLQAGKAYGLAPGSWVGPYAMCRTWE 997
            RSWRK +DKP++  Y +IL LFGDS  +A+SIHNLLQAGK YGLA GSWVGPYAMCRTWE
Sbjct: 194  RSWRKTVDKPVDKEYIDILQLFGDSEAAAFSIHNLLQAGKGYGLAVGSWVGPYAMCRTWE 253

Query: 998  TLSRCMRKESENSGLTSAMSIYVVSGDEDGERGGAPVVCIEDIYNHCVVSSN--MDWTXX 1171
             L+R  R+++E       M+IYVVSGDEDGERGGAPVVCIED    C+  S   + WT  
Sbjct: 254  VLARNQREKNEQGEQLLPMAIYVVSGDEDGERGGAPVVCIEDACKRCLEFSRGLVPWTPL 313

Query: 1172 XXXXXXXXXXDKINPRYLPLLGSTFTFPQSLGILGGRPGVSTYIIGVQDEKAFYLDPHET 1351
                      DK+N RY+PLL STF FPQSLGILGG+PG STYIIGVQ++KAFYLDPHE 
Sbjct: 314  LLLVPLVLGLDKVNLRYIPLLQSTFKFPQSLGILGGKPGASTYIIGVQNDKAFYLDPHEV 373

Query: 1352 QPVVDIMGDCSEVDTSSFHCSIIRQFPLDSIDPSLAIGFYCRDENDFADFCSRAIELADQ 1531
            +PVV+I GD  E +TSS+HC+I R  PLDSIDPSLAIGFYCRD++DF DFCSRA +LA++
Sbjct: 374  KPVVNITGDTQEPNTSSYHCNISRHMPLDSIDPSLAIGFYCRDKDDFDDFCSRATKLAEE 433

Query: 1532 SNGAPLFTITETRKSAKSFSQNDISSDNEMINTDSQFNGVQRGESTRDTQDEDWQFL 1702
            SNGAPLFT+ ++R      + N +S D+     D   +     ++     ++DWQFL
Sbjct: 434  SNGAPLFTVAQSRSLPMQVTSNSVSGDDTRFEEDDSLSMNLVNDA---GNEDDWQFL 487


>ref|XP_002331599.1| predicted protein [Populus trichocarpa] gi|222873995|gb|EEF11126.1|
            predicted protein [Populus trichocarpa]
          Length = 482

 Score =  514 bits (1323), Expect = e-143
 Identities = 264/471 (56%), Positives = 325/471 (69%), Gaps = 12/471 (2%)
 Frame = +2

Query: 326  DSPKQLIGSVSFEAGPSSTKDYKASVWSGFLVQ--SIFDT-CNQSKNYTKKTSNT---NG 487
            +SP +   S S E G + TK  K S+WS F     S+FDT C+ S    KK  +    NG
Sbjct: 20   ESPNRSFTSDSSELGSADTKFSKPSLWSTFFASAFSVFDTHCDSSSTSEKKAPHIRHGNG 79

Query: 488  WTAAVRKVMYSGTMRRI----FGLNRASVSGSRTDIWLLGVCYRVAQDDPSADPSQSEGY 655
            WT+AV+K++  G+MRRI     G ++  +S +  DIWLLG CY+++QD+ S D + +   
Sbjct: 80   WTSAVKKIVAGGSMRRIQECVLGTSKTGISNTTGDIWLLGACYKISQDNSSGDAAATNAL 139

Query: 656  AAFVEDFSSRICITYRKGFAAIEDTKYTSDAGWGCMLRSSQMLVAQALLVHQLGRSWRKA 835
            AAF  DFSSRI ITYRKGF AIED+K TSD  WGCMLRSSQMLVAQALL H+LGRSWRK 
Sbjct: 140  AAFNHDFSSRILITYRKGFDAIEDSKLTSDVSWGCMLRSSQMLVAQALLFHRLGRSWRKP 199

Query: 836  LDKPLEPTYFEILHLFGDSMISAYSIHNLLQAGKAYGLAPGSWVGPYAMCRTWETLSRCM 1015
            LDKPL+  Y EILHLFGDS  SA+SIHNLL+AGKAYGLA GSWVGPYA+C +WE+L R  
Sbjct: 200  LDKPLDREYVEILHLFGDSESSAFSIHNLLRAGKAYGLAAGSWVGPYAVCHSWESLVRSR 259

Query: 1016 RKESENSGLTSAMSIYVVSGDEDGERGGAPVVCIEDIYNHCVVSS--NMDWTXXXXXXXX 1189
            R+E+     + +M++YVVSG EDGERGGAPV+CIE+   HC   S    DWT        
Sbjct: 260  REETNLEYQSLSMAVYVVSGSEDGERGGAPVLCIEEAARHCSEFSKGQEDWTPILLLVPL 319

Query: 1190 XXXXDKINPRYLPLLGSTFTFPQSLGILGGRPGVSTYIIGVQDEKAFYLDPHETQPVVDI 1369
                DKINPRY+P L +TFTFPQSLGILGG+PG STYI+GVQDE AFYLDPHE QPVV++
Sbjct: 320  VLGLDKINPRYIPSLQATFTFPQSLGILGGKPGASTYIVGVQDENAFYLDPHEVQPVVNV 379

Query: 1370 MGDCSEVDTSSFHCSIIRQFPLDSIDPSLAIGFYCRDENDFADFCSRAIELADQSNGAPL 1549
              D  E +TSS+HC+++R  PLD IDPSLAIGFYCRD++DF DFC+ A +L D+SNGAPL
Sbjct: 380  SRDDVEANTSSYHCNVVRHMPLDLIDPSLAIGFYCRDKDDFDDFCTLASKLTDESNGAPL 439

Query: 1550 FTITETRKSAKSFSQNDISSDNEMINTDSQFNGVQRGESTRDTQDEDWQFL 1702
            FT+  +RK  K  S    S D+  + T +   G           ++DWQ L
Sbjct: 440  FTVAHSRKLLKHDSGEVRSDDSLGVMTMNDVEGC--------VHEDDWQLL 482


>ref|XP_003552483.1| PREDICTED: cysteine protease ATG4-like [Glycine max]
          Length = 485

 Score =  513 bits (1320), Expect = e-143
 Identities = 274/490 (55%), Positives = 340/490 (69%), Gaps = 17/490 (3%)
 Frame = +2

Query: 284  VMKG--EGIHNLECSFDSPKQLIGS----VSFEAGPSSTKDYKASVWSGFLVQ--SIFDT 439
            V+KG  E I + +CS  S  + + +    V  +AG S  K  KAS+WS       S+ +T
Sbjct: 2    VLKGLCERIVSSKCSSKSSTETVDNTQVPVYSKAGSSDCKFPKASLWSSIFTSGFSVVET 61

Query: 440  CNQSKNYTKKT--SNTNGWTAAVRKVMYSGTMRR----IFGLNRASVSGSRTDIWLLGVC 601
             ++S    KK   S ++GW AAVRKV+  G+MRR    + G +R  +S S  DIWLLGVC
Sbjct: 62   YSESSASEKKAVPSRSSGWAAAVRKVVTGGSMRRFQERVLGSSRTDISSSDGDIWLLGVC 121

Query: 602  YRVAQDDPSADPSQSEGYAAFVEDFSSRICITYRKGFAAIEDTKYTSDAGWGCMLRSSQM 781
            ++++Q + +     S G A+F +DFSS+I +TYRKGF AI DTKYTSD  WGCMLRSSQM
Sbjct: 122  HKISQQESTGGVDTSNGLASFEQDFSSKILVTYRKGFDAIGDTKYTSDVNWGCMLRSSQM 181

Query: 782  LVAQALLVHQLGRSWRKALDKPLEPTYFEILHLFGDSMISAYSIHNLLQAGKAYGLAPGS 961
            LVAQALL H+LGRSWRK +DKPL+  Y ++L LFGDS  SA+SIHNLLQAGK YGLA GS
Sbjct: 182  LVAQALLFHKLGRSWRKPIDKPLDKEYIDVLQLFGDSEASAFSIHNLLQAGKGYGLAVGS 241

Query: 962  WVGPYAMCRTWETLSRCMRKESENSGLTSAMSIYVVSGDEDGERGGAPVVCIEDIYNHCV 1141
            WVGPYAMCRTWE L+   RK+++       M+IYVVSGDEDGERGGAPVVCIED    C 
Sbjct: 242  WVGPYAMCRTWEVLA---RKKNDLGEPPLPMAIYVVSGDEDGERGGAPVVCIEDASKRCS 298

Query: 1142 VSSN--MDWTXXXXXXXXXXXXDKINPRYLPLLGSTFTFPQSLGILGGRPGVSTYIIGVQ 1315
              S+    WT            DK+NPRY+PLL STF FPQSLGI+GG+PG STYIIGVQ
Sbjct: 299  EFSSGLAVWTPLLLLVPLVLGLDKVNPRYIPLLRSTFKFPQSLGIMGGKPGASTYIIGVQ 358

Query: 1316 DEKAFYLDPHETQPVVDIMGDCSE-VDTSSFHCSIIRQFPLDSIDPSLAIGFYCRDENDF 1492
            +EKAFYLDPH+ Q VV+I GD  E   TSS+HC+++R  PLDSIDPSLAIGFYCRD++DF
Sbjct: 359  NEKAFYLDPHDVQQVVNISGDTQEPTGTSSYHCNVMRHIPLDSIDPSLAIGFYCRDKDDF 418

Query: 1493 ADFCSRAIELADQSNGAPLFTITETRKSAKSFSQNDISSDNEMINTDSQFNGVQRGESTR 1672
             DFCS+A +LA++SNGAPLFT+ ++R  +K  S ND+S DN     D  F G+  G  T 
Sbjct: 419  DDFCSQASKLAEESNGAPLFTVAKSRSFSKQVS-NDVSGDNTGFQED-DFPGMDCGNDT- 475

Query: 1673 DTQDEDWQFL 1702
             T ++DWQ L
Sbjct: 476  VTNEDDWQLL 485


>ref|XP_002309707.1| predicted protein [Populus trichocarpa] gi|222852610|gb|EEE90157.1|
            predicted protein [Populus trichocarpa]
          Length = 481

 Score =  510 bits (1314), Expect = e-142
 Identities = 262/471 (55%), Positives = 321/471 (68%), Gaps = 12/471 (2%)
 Frame = +2

Query: 326  DSPKQLIGSVSFEAGPSSTKDYKASVWSGFLVQ--SIFD----TCNQSKNYTKKTSNTNG 487
            D+PK    S S E G + TK  K S+WS F     S+FD    + + S N      ++NG
Sbjct: 19   DTPKSSFISDSSEPGSTDTKVSKPSLWSSFFASAFSVFDIYRDSSSTSHNEAPHIRHSNG 78

Query: 488  WTAAVRKVMYSGTMRRI----FGLNRASVSGSRTDIWLLGVCYRVAQDDPSADPSQSEGY 655
            WT++V+K++  GTMRRI     G ++  +S + +DIWLLG  Y+++QDD S +   +   
Sbjct: 79   WTSSVKKIVAGGTMRRIQERVLGTSKTGISNTTSDIWLLGARYKISQDDSSGNADATNAL 138

Query: 656  AAFVEDFSSRICITYRKGFAAIEDTKYTSDAGWGCMLRSSQMLVAQALLVHQLGRSWRKA 835
            AAF  DFSSRI ITYRKGF  IED+K TSD  WGCMLRSSQMLVAQALL H+LGRSWRK 
Sbjct: 139  AAFHRDFSSRILITYRKGFDMIEDSKLTSDVNWGCMLRSSQMLVAQALLFHRLGRSWRKP 198

Query: 836  LDKPLEPTYFEILHLFGDSMISAYSIHNLLQAGKAYGLAPGSWVGPYAMCRTWETLSRCM 1015
            +DKPL+  Y EILHLFGDS  SA+SIHNLLQAGKAYGLA GSWVGPYAMCR+WE+L+R  
Sbjct: 199  VDKPLDRDYVEILHLFGDSEASAFSIHNLLQAGKAYGLAAGSWVGPYAMCRSWESLARSK 258

Query: 1016 RKESENSGLTSAMSIYVVSGDEDGERGGAPVVCIEDIYNHCVVSS--NMDWTXXXXXXXX 1189
            R+E+     T  M++YVVSG EDGERGGAPV+ IED   HC   S    DWT        
Sbjct: 259  REETNLEYQTLPMAVYVVSGCEDGERGGAPVLSIEDAARHCSEFSKGREDWTPILLLVPL 318

Query: 1190 XXXXDKINPRYLPLLGSTFTFPQSLGILGGRPGVSTYIIGVQDEKAFYLDPHETQPVVDI 1369
                DKINPRY+P L +TFTFPQSLGILGG+PG STYI+GVQDE AFYLDPHE QPVV+ 
Sbjct: 319  VLGLDKINPRYIPSLQATFTFPQSLGILGGKPGASTYIVGVQDENAFYLDPHEVQPVVNF 378

Query: 1370 MGDCSEVDTSSFHCSIIRQFPLDSIDPSLAIGFYCRDENDFADFCSRAIELADQSNGAPL 1549
              D  E +TSS+HC ++R  PLD IDPSLAIGFYCRD++DF DFCS A +LAD+SNGAPL
Sbjct: 379  SRDDVEANTSSYHCDVVRHIPLDLIDPSLAIGFYCRDKDDFDDFCSLASKLADESNGAPL 438

Query: 1550 FTITETRKSAKSFSQNDISSDNEMINTDSQFNGVQRGESTRDTQDEDWQFL 1702
            FT+  + KS+K         D+  +  D     +   ++     ++DWQ L
Sbjct: 439  FTVANSYKSSK--------HDSSEVRDDDPLGVMTMNDAEGCLNEDDWQLL 481