BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum22_contig00005816
         (1863 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFP49810.1| 4-hydroxycinnamoyl-CoA ligase 3 [Coffea arabica]       706   0.0  
sp|O24146.1|4CL2_TOBAC RecName: Full=4-coumarate--CoA ligase 2; ...   706   0.0  
sp|O24145.1|4CL1_TOBAC RecName: Full=4-coumarate--CoA ligase 1; ...   704   0.0  
ref|XP_002533186.1| AMP dependent CoA ligase, putative [Ricinus ...   703   0.0  
gb|AFU90743.1| 4-coumarate:coenzyme A ligase [Punica granatum]        700   0.0  

>gb|AFP49810.1| 4-hydroxycinnamoyl-CoA ligase 3 [Coffea arabica]
          Length = 543

 Score =  706 bits (1823), Expect = 0.0
 Identities = 362/546 (66%), Positives = 436/546 (79%), Gaps = 2/546 (0%)
 Frame = -3

Query: 1822 METKEIQ-EFIFKSPLPDITLKNHLPLHTYIFQNISQFRSSQCLINSVTGEIFTYDDVEV 1646
            METK+ Q EF+F+S LPDI + NHLPLH+Y F+NI +F +  CLIN  TG+I+TY DVE+
Sbjct: 1    METKQDQQEFVFRSKLPDIYIPNHLPLHSYCFENIPEFCNRPCLINGTTGDIYTYADVEL 60

Query: 1645 ATKKVAIGFRNKLGIQKGDVIMILLHNSPEFVFSFLGASFCGAISTTANPLYTIQEIGKQ 1466
              +KVA G  +K+GIQ+G+VIM+LL NSPEF F+ LGAS+ GA+STTANP Y   EI KQ
Sbjct: 61   IARKVAAGL-DKIGIQQGEVIMLLLQNSPEFAFALLGASYRGAMSTTANPFYKPAEIEKQ 119

Query: 1465 AKGSRAKVIITHACYVEKVRDFAQENDVKIVCVDDSTPEGCFSFCELKEINENDELPEVD 1286
            A+ S+AK+IIT +CYVEKV DFA+EN+VK++C D + PEGC  F EL   +E   +P V 
Sbjct: 120  ARASKAKLIITQSCYVEKVMDFAKENNVKVMCTD-APPEGCLHFSELSSADEK-VIPAVK 177

Query: 1285 INPDEDLVALPYSSGTTGLPKGVMLTHKNLVSSVSVLVDGENPNMNFHKEDSIICVLPLF 1106
            INP+ D VALPYSSGTTGLPKGVMLTHK LV+SV+  VDGENPN+ FHKED I+CVLPLF
Sbjct: 178  INPN-DAVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHKEDVILCVLPLF 236

Query: 1105 HIYCLNSVFLTALRVGATLVIMQKFDINNCMEVVEKYKVTITPFVPPILLAIIKSELADK 926
            HIY LNSV L  LRVGA ++IMQKF+IN  ME+V+KYKVTI PFVPPI+L I KS + DK
Sbjct: 237  HIYSLNSVLLCGLRVGAAILIMQKFEINALMELVQKYKVTIAPFVPPIVLEIAKSPVVDK 296

Query: 925  YDLSSIRMVQSGGAPLGKELEDAIRTKLPSAVLGQGCGMTEAGPL-AMCPMFAKEPASDA 749
            YDLSSIRMV SG AP+GKELED +R KLP AVLGQG GMTEAGPL +MC  FAKEP  D 
Sbjct: 297  YDLSSIRMVMSGAAPMGKELEDTVRAKLPKAVLGQGYGMTEAGPLLSMCLAFAKEPF-DV 355

Query: 748  KSGSCGKVVRNIEMKIVDIQTGASLPHNQSGEICIRGDQVMKGYLDDPENTARAIDKEGW 569
            KSG+CG VVRN EMKIVD +T  SLP NQ+GEICIRGDQ+MKGYL+DPE T   IDKEGW
Sbjct: 356  KSGACGTVVRNAEMKIVDPETNLSLPRNQAGEICIRGDQIMKGYLNDPEATENTIDKEGW 415

Query: 568  MHSXXXXXXXXXXXXXXXDRLKEIIKYKGFQVAPAELESLLVAHPSISEAVVVPMKDEAA 389
            +H+               DRLKE+IKYKGFQVAPAELE++L++HP IS+A VV MKDEAA
Sbjct: 416  LHTGDIGYIDDDDEIFIVDRLKELIKYKGFQVAPAELEAMLLSHPGISDAAVVSMKDEAA 475

Query: 388  GEVPVAFVVKANGSQVTEEEIKNYIAQQVIYYKKIKRVFFVDAIPKAPSGKLLRKEVRTK 209
            GEVPVAFVV+A+GS+++E+EIK +I+ QVI+YK+I RVFF+D IPKAPSGK+LRK++R K
Sbjct: 476  GEVPVAFVVRASGSKISEDEIKQFISNQVIFYKRIHRVFFMDKIPKAPSGKILRKDLRAK 535

Query: 208  LLAGLA 191
            L A +A
Sbjct: 536  LAAEVA 541


>sp|O24146.1|4CL2_TOBAC RecName: Full=4-coumarate--CoA ligase 2; Short=4CL 2; AltName:
            Full=4-coumaroyl-CoA synthase 2 gi|1663724|gb|AAB18638.1|
            4-coumarate:coenzyme A ligase [Nicotiana tabacum]
          Length = 542

 Score =  706 bits (1821), Expect = 0.0
 Identities = 360/545 (66%), Positives = 439/545 (80%), Gaps = 1/545 (0%)
 Frame = -3

Query: 1825 QMETKEIQEFIFKSPLPDITLKNHLPLHTYIFQNISQFRSSQCLINSVTGEIFTYDDVEV 1646
            + +TK++ + IF+S LPDI + NHLPLH+Y F+NIS+F S  CLIN    +I+TY DVE+
Sbjct: 2    EKDTKQV-DIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVEL 60

Query: 1645 ATKKVAIGFRNKLGIQKGDVIMILLHNSPEFVFSFLGASFCGAISTTANPLYTIQEIGKQ 1466
             ++KVA G  +K GIQ  D IMILL NSPEFVF+F+GAS+ GAIST ANPL+T  E+ KQ
Sbjct: 61   NSRKVAAGL-HKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQ 119

Query: 1465 AKGSRAKVIITHACYVEKVRDFAQENDVKIVCVDDSTPEGCFSFCELKEINENDELPEVD 1286
            AK S AK+I+T AC+V KV+D+A ENDVKI+C+D S PEGC  F  L + NE+D +PEV+
Sbjct: 120  AKASSAKIIVTQACHVNKVKDYAFENDVKIICID-SAPEGCLHFSVLTQANEHD-IPEVE 177

Query: 1285 INPDEDLVALPYSSGTTGLPKGVMLTHKNLVSSVSVLVDGENPNMNFHKEDSIICVLPLF 1106
            I PD D+VALPYSSGTTGLPKGVMLTHK LV+SV+  VDGENPN+  H ED ++CVLPLF
Sbjct: 178  IQPD-DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLF 236

Query: 1105 HIYCLNSVFLTALRVGATLVIMQKFDINNCMEVVEKYKVTITPFVPPILLAIIKSELADK 926
            HIY LNSV L  LRVGA ++IMQKFDI + +E++++YKVTI PFVPPI+LAI KS + D 
Sbjct: 237  HIYSLNSVLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDD 296

Query: 925  YDLSSIRMVQSGGAPLGKELEDAIRTKLPSAVLGQGCGMTEAGP-LAMCPMFAKEPASDA 749
            YDLSS+R V SG APLGKELED +R K P+A LGQG GMTEAGP LAMC  FAKEP  + 
Sbjct: 297  YDLSSVRTVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF-EI 355

Query: 748  KSGSCGKVVRNIEMKIVDIQTGASLPHNQSGEICIRGDQVMKGYLDDPENTARAIDKEGW 569
            KSG+CG VVRN EMKIVD +TG SLP NQSGEICIRGDQ+MKGYL+DPE TAR IDKEGW
Sbjct: 356  KSGACGTVVRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGW 415

Query: 568  MHSXXXXXXXXXXXXXXXDRLKEIIKYKGFQVAPAELESLLVAHPSISEAVVVPMKDEAA 389
            +++               DRLKE+IKYKGFQVAPAELE+LL+ HP+IS+A VVPMKDE A
Sbjct: 416  LYTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQA 475

Query: 388  GEVPVAFVVKANGSQVTEEEIKNYIAQQVIYYKKIKRVFFVDAIPKAPSGKLLRKEVRTK 209
            GEVPVAFVV++NGS +TE+E+K++I++QVI+YK+IKRVFFVDAIPK+PSGK+LRK++R K
Sbjct: 476  GEVPVAFVVRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAK 535

Query: 208  LLAGL 194
            L AGL
Sbjct: 536  LAAGL 540


>sp|O24145.1|4CL1_TOBAC RecName: Full=4-coumarate--CoA ligase 1; Short=4CL 1; AltName:
            Full=4-coumaroyl-CoA synthase 1 gi|1663722|gb|AAB18637.1|
            4-coumarate:coenzyme A ligase [Nicotiana tabacum]
          Length = 547

 Score =  704 bits (1817), Expect = 0.0
 Identities = 354/546 (64%), Positives = 435/546 (79%), Gaps = 1/546 (0%)
 Frame = -3

Query: 1828 TQMETKEIQEFIFKSPLPDITLKNHLPLHTYIFQNISQFRSSQCLINSVTGEIFTYDDVE 1649
            T  ETK+  + IF+S LPDI +  HLPLH+Y F+NIS+F S  CLIN    +I+TY +VE
Sbjct: 5    TTTETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAEVE 64

Query: 1648 VATKKVAIGFRNKLGIQKGDVIMILLHNSPEFVFSFLGASFCGAISTTANPLYTIQEIGK 1469
            +  +KVA+G  NKLGIQ+ D IMILL NSPEFVF+F+GAS+ GAIST ANPL+T  E+ K
Sbjct: 65   LTCRKVAVGL-NKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVVK 123

Query: 1468 QAKGSRAKVIITHACYVEKVRDFAQENDVKIVCVDDSTPEGCFSFCELKEINENDELPEV 1289
            QAK S AK+IIT +C+V KV+D+A ENDVK++C+D S PEGC  F EL + +E+ E+PEV
Sbjct: 124  QAKASSAKIIITQSCFVGKVKDYASENDVKVICID-SAPEGCLHFSELTQSDEH-EIPEV 181

Query: 1288 DINPDEDLVALPYSSGTTGLPKGVMLTHKNLVSSVSVLVDGENPNMNFHKEDSIICVLPL 1109
             I PD D+VALPYSSGTTGLPKGVMLTHK LV+SV+  VDGEN N+  H ED ++CVLPL
Sbjct: 182  KIQPD-DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPL 240

Query: 1108 FHIYCLNSVFLTALRVGATLVIMQKFDINNCMEVVEKYKVTITPFVPPILLAIIKSELAD 929
            FHIY LNS+ L  LRVGA ++IMQKFDI   +E+++KYKV+I PFVPPI+LAI KS + D
Sbjct: 241  FHIYSLNSILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVD 300

Query: 928  KYDLSSIRMVQSGGAPLGKELEDAIRTKLPSAVLGQGCGMTEAGP-LAMCPMFAKEPASD 752
             YDLSS+R V SG APLGKELEDA+RTK P+A LGQG GMTEAGP LAMC  FAKEP  D
Sbjct: 301  SYDLSSVRTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF-D 359

Query: 751  AKSGSCGKVVRNIEMKIVDIQTGASLPHNQSGEICIRGDQVMKGYLDDPENTARAIDKEG 572
             KSG+CG VVRN EMKIVD  TG SLP NQ GEICIRGDQ+MKGYL+DPE T R IDKEG
Sbjct: 360  IKSGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEG 419

Query: 571  WMHSXXXXXXXXXXXXXXXDRLKEIIKYKGFQVAPAELESLLVAHPSISEAVVVPMKDEA 392
            W+H+               DRLKE+IKYKGFQVAPAE+E+LL+ HP+IS+A VVPMKDE 
Sbjct: 420  WLHTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQ 479

Query: 391  AGEVPVAFVVKANGSQVTEEEIKNYIAQQVIYYKKIKRVFFVDAIPKAPSGKLLRKEVRT 212
            AGEVPVAFVV++NGS +TE+E+K++I++QVI+YK++KRVFFV+ +PK+PSGK+LRK++R 
Sbjct: 480  AGEVPVAFVVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRA 539

Query: 211  KLLAGL 194
            +L AG+
Sbjct: 540  RLAAGV 545


>ref|XP_002533186.1| AMP dependent CoA ligase, putative [Ricinus communis]
            gi|223527010|gb|EEF29200.1| AMP dependent CoA ligase,
            putative [Ricinus communis]
          Length = 557

 Score =  703 bits (1815), Expect = 0.0
 Identities = 357/543 (65%), Positives = 432/543 (79%), Gaps = 6/543 (1%)
 Frame = -3

Query: 1804 QEFIFKSPLPDITLKNHLPLHTYIFQNISQFRSSQCLINSVTGEIFTYDDVEVATKKVAI 1625
            QEFIF+S LPDI + NHLPLHTY F+NISQF+ + CLIN  TG+++TY DVE+ ++KVA 
Sbjct: 17   QEFIFRSKLPDIYIPNHLPLHTYCFENISQFKDNACLINGATGDVYTYADVELTSRKVAA 76

Query: 1624 GFRNKLGIQKGDVIMILLHNSPEFVFSFLGASFCGAISTTANPLYTIQEIGKQAKGSRAK 1445
            G  +KLGI++ +VIMILL NSPEFVF+FLGAS  GAISTTANP YT  EI KQA  S AK
Sbjct: 77   GL-HKLGIKQNEVIMILLQNSPEFVFAFLGASVLGAISTTANPFYTPAEIKKQASASNAK 135

Query: 1444 VIITHACYVEKVRDFAQENDVKIVCVDDSTP-----EGCFSFCELKEINENDELPEVDIN 1280
            +IIT A YVEKV+DFA EN +KI+ +D   P       C  F EL + +END +P V IN
Sbjct: 136  LIITQAAYVEKVKDFANENGIKIMTIDSPPPALDDCRDCLHFSELTKADEND-IPAVKIN 194

Query: 1279 PDEDLVALPYSSGTTGLPKGVMLTHKNLVSSVSVLVDGENPNMNFHKEDSIICVLPLFHI 1100
            PD D+VALPYSSGTTGLPKGVMLTHK+LV+SV+  VDGENPN+ FH++D I+C+LPLFHI
Sbjct: 195  PD-DVVALPYSSGTTGLPKGVMLTHKSLVTSVAQQVDGENPNLYFHEKDVILCLLPLFHI 253

Query: 1099 YCLNSVFLTALRVGATLVIMQKFDINNCMEVVEKYKVTITPFVPPILLAIIKSELADKYD 920
            Y LNSV L  LRVGA ++IMQKF+I   ME+V+KYKVTI PFVPPI+L+I KS   DKYD
Sbjct: 254  YSLNSVLLCGLRVGAAILIMQKFEITALMELVQKYKVTIAPFVPPIVLSIAKSPAVDKYD 313

Query: 919  LSSIRMVQSGGAPLGKELEDAIRTKLPSAVLGQGCGMTEAGP-LAMCPMFAKEPASDAKS 743
            LSSIR V SG AP+GKELED +R KLP+A LGQG GMTEAGP L+MC  FAKEP  + KS
Sbjct: 314  LSSIRTVMSGAAPMGKELEDTVRAKLPNAKLGQGYGMTEAGPVLSMCLAFAKEP-FEIKS 372

Query: 742  GSCGKVVRNIEMKIVDIQTGASLPHNQSGEICIRGDQVMKGYLDDPENTARAIDKEGWMH 563
            G+CG VVRN EMKIVD  TG SL  NQ+GEICIRG Q+MKGYL+DPE T R IDKEGW+H
Sbjct: 373  GACGTVVRNAEMKIVDPDTGKSLQRNQAGEICIRGSQIMKGYLNDPEATERTIDKEGWLH 432

Query: 562  SXXXXXXXXXXXXXXXDRLKEIIKYKGFQVAPAELESLLVAHPSISEAVVVPMKDEAAGE 383
            +               DRLKE+IKY GFQVAPAELE++L+AHPSIS+A VVPMKDEAAGE
Sbjct: 433  TGDVGYIDGDDELFIVDRLKELIKYNGFQVAPAELEAMLIAHPSISDAAVVPMKDEAAGE 492

Query: 382  VPVAFVVKANGSQVTEEEIKNYIAQQVIYYKKIKRVFFVDAIPKAPSGKLLRKEVRTKLL 203
            +P AFVV++NGS++TE++++ YI++QVIYYK+I+RVFF D+IPKAPSGK+LRK++R +L 
Sbjct: 493  IPAAFVVRSNGSKITEDDVQQYISKQVIYYKRIRRVFFTDSIPKAPSGKILRKDLRARLA 552

Query: 202  AGL 194
            AG+
Sbjct: 553  AGV 555


>gb|AFU90743.1| 4-coumarate:coenzyme A ligase [Punica granatum]
          Length = 544

 Score =  700 bits (1807), Expect = 0.0
 Identities = 357/548 (65%), Positives = 440/548 (80%), Gaps = 4/548 (0%)
 Frame = -3

Query: 1822 METKE---IQEFIFKSPLPDITLKNHLPLHTYIFQNISQFRSSQCLINSVTGEIFTYDDV 1652
            METK+    QEFIF+S LPDI + NHLPLH+Y F+NIS+F+   CLI+  TG I+TY +V
Sbjct: 1    METKQDTVSQEFIFRSKLPDIYIPNHLPLHSYCFENISEFQDRPCLIDGSTGRIYTYSEV 60

Query: 1651 EVATKKVAIGFRNKLGIQKGDVIMILLHNSPEFVFSFLGASFCGAISTTANPLYTIQEIG 1472
            E+ +++VA G  NKLGI++G VIM+LLHNSPEFV +FLGASFCGA+STTANP  T  EI 
Sbjct: 61   ELTSRRVASGL-NKLGIEQGQVIMLLLHNSPEFVHAFLGASFCGAMSTTANPFCTPPEIA 119

Query: 1471 KQAKGSRAKVIITHACYVEKVRDFAQENDVKIVCVDDSTPEGCFSFCELKEINENDELPE 1292
            KQA  S+A++IIT A +  KV+DFA+EN VK++C+D S PEGC  F EL + +E  E+P 
Sbjct: 120  KQAAASKARLIITQAEFAPKVKDFAEENGVKVMCID-SAPEGCLHFSELTQADEA-EMPS 177

Query: 1291 VDINPDEDLVALPYSSGTTGLPKGVMLTHKNLVSSVSVLVDGENPNMNFHKEDSIICVLP 1112
            V I+P+ D+VALPYSSGTTGLPKGVMLTHK LV+SV+  VDGENPN+ F KED I+CVLP
Sbjct: 178  VKIDPN-DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFRKEDVILCVLP 236

Query: 1111 LFHIYCLNSVFLTALRVGATLVIMQKFDINNCMEVVEKYKVTITPFVPPILLAIIKSELA 932
            LFHIY LNS+ L  LRVGA ++IMQKF+I   ME+V++YKVTI PFVPPI+LA+ K    
Sbjct: 237  LFHIYSLNSIMLCGLRVGAAILIMQKFEIVKLMELVQRYKVTIAPFVPPIVLAMSKYADM 296

Query: 931  DKYDLSSIRMVQSGGAPLGKELEDAIRTKLPSAVLGQGCGMTEAGP-LAMCPMFAKEPAS 755
            DKYDLSSIR V SG AP+GKELED++R KLP+A LGQG GMTEAGP LAMC  FAKEP  
Sbjct: 297  DKYDLSSIRTVMSGAAPMGKELEDSVRAKLPNAKLGQGYGMTEAGPVLAMCLAFAKEP-F 355

Query: 754  DAKSGSCGKVVRNIEMKIVDIQTGASLPHNQSGEICIRGDQVMKGYLDDPENTARAIDKE 575
            + KSG+CG VVRN EMKIV+  TGASLP NQ GEICIRG+Q+MKGYL+DPE T R IDK+
Sbjct: 356  EIKSGACGTVVRNAEMKIVNPDTGASLPRNQPGEICIRGNQIMKGYLNDPEATERTIDKD 415

Query: 574  GWMHSXXXXXXXXXXXXXXXDRLKEIIKYKGFQVAPAELESLLVAHPSISEAVVVPMKDE 395
            GW+H+               DRLKE+IKYKGFQVAPAELE++L+AHPSIS+A VVPMKDE
Sbjct: 416  GWLHTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEAMLIAHPSISDAAVVPMKDE 475

Query: 394  AAGEVPVAFVVKANGSQVTEEEIKNYIAQQVIYYKKIKRVFFVDAIPKAPSGKLLRKEVR 215
            AAGEVPVAFV+++NGS++TE++IK YI++QV++YK+I RVFF +AIPKAPSGK+LRK +R
Sbjct: 476  AAGEVPVAFVIRSNGSKITEDDIKQYISKQVVFYKRINRVFFTEAIPKAPSGKILRKYLR 535

Query: 214  TKLLAGLA 191
             KL +G++
Sbjct: 536  AKLASGVS 543