BLASTX nr result
ID: Lithospermum22_contig00004804
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Lithospermum22_contig00004804
(1737 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|AFK79031.1| cytochrome P450 CYP736A54 [Bupleurum chinense] 654 0.0
ref|XP_002532045.1| flavonoid 3-hydroxylase, putative [Ricinus c... 626 e-177
ref|XP_002280620.1| PREDICTED: cytochrome P450 71A1 [Vitis vinif... 622 e-175
ref|XP_003524281.1| PREDICTED: cytochrome P450 71A1-like [Glycin... 619 e-175
ref|XP_002310008.1| cytochrome P450 [Populus trichocarpa] gi|222... 607 e-171
>gb|AFK79031.1| cytochrome P450 CYP736A54 [Bupleurum chinense]
Length = 497
Score = 654 bits (1686), Expect = 0.0
Identities = 313/504 (62%), Positives = 397/504 (78%)
Frame = +3
Query: 120 MALIWKTILVVIAITALLDRFLKKIIKKRRVPLPPSXXXXXXXXXXXXXXXNPHQDLHKL 299
MA W T+L ++ + L+ +LKK +++ LPP NPHQDL KL
Sbjct: 1 MAWTWITLLTLVVLAYLVQSWLKKKTQRK---LPPGPKGLPIIGHLHMLGKNPHQDLQKL 57
Query: 300 SKEHGPIMYMKFGMIPNIIVSSPDAAEQFLKTHDLVFASRPPHEAAKYISYDQTNLSFAP 479
+++HGPIM M+FG +PNIIVSSP+AA+QFLKTHDL FA RP EAAKYISY+Q NLSF+
Sbjct: 58 AEKHGPIMSMRFGFVPNIIVSSPEAAKQFLKTHDLNFAGRPSLEAAKYISYEQRNLSFST 117
Query: 480 YGHYWRNMRKLCTLELLSNMKINSFHPMRSEEISNFIDLLKSCSQDRSVVDLSSQVSTLN 659
YG YWRNMRKLCTLELLSN+KINSF MR +EI+N +D+++ +Q+R VD+S ++S++N
Sbjct: 118 YGPYWRNMRKLCTLELLSNLKINSFQAMRKKEIANVVDIIEQAAQERVAVDISQRISSMN 177
Query: 660 TNMTCRMIFGKKYTNEEFGDKKGFEGVIQEGMQLAAKPNLGDYFPYLGKLDLQGLTARFK 839
++++C+M+FGKK+ ++EF D++GF+GVIQ GMQ+A NLGDYFPYLG LDLQGL R K
Sbjct: 178 SDISCQMVFGKKFEDKEF-DERGFKGVIQVGMQMAVAFNLGDYFPYLGALDLQGLNKRMK 236
Query: 840 AVSAVFDAFFEKVLDEHEQQSSRGHENQEGKNMDFVDTMLGILTSGQAEFTFDRRHVKAV 1019
A++ V+D F EK+LD+H+Q + G+ DFVDTML I+ SG++EF FDR HVKA
Sbjct: 237 AIAKVWDQFLEKILDDHDQSK------EPGQTKDFVDTMLDIMKSGESEFEFDRSHVKAT 290
Query: 1020 LLDMLAGAMDTSATVIDWTMSELLRHPTIMKNVQSELEQVVGLNRIVEESDLGKLEYLNM 1199
L+DM A + DTS+T I+WT+SELLRHP +M VQ ELEQVVG+NR+VEESDL LEYL M
Sbjct: 291 LMDMFAASADTSSTTIEWTLSELLRHPRVMNKVQKELEQVVGMNRMVEESDLESLEYLGM 350
Query: 1200 VVKESMRLHPVAPLLIPHEALEDCTVEGYHIPKKSRVIINAWSIGRDPEAWSDPEKFIPK 1379
V+KE+MRLHPVAPLL+PH A+EDCTV+G+ IPK SRV++N W+IGRD WSD EKF+P+
Sbjct: 351 VIKETMRLHPVAPLLLPHLAIEDCTVDGFFIPKNSRVVVNVWAIGRDSNVWSDAEKFLPE 410
Query: 1380 RFQGSKIDVRGRDFQLIPFGSGRRGCPGIQLGLIVVSLVVAQLVHCFDWELPNGMLNTEL 1559
RF GS ID+RGRDF+L+PFGSGRRGCPG+QLGL VV LVVAQL+HCFDW+LPNGM +EL
Sbjct: 411 RFIGSNIDLRGRDFELLPFGSGRRGCPGMQLGLTVVRLVVAQLLHCFDWDLPNGMQPSEL 470
Query: 1560 DMSEEFGLVTTRAGHLMAIPTYRL 1631
DM+EEFGL+ RA HLMAIPT RL
Sbjct: 471 DMTEEFGLLVGRAKHLMAIPTCRL 494
>ref|XP_002532045.1| flavonoid 3-hydroxylase, putative [Ricinus communis]
gi|223528288|gb|EEF30335.1| flavonoid 3-hydroxylase,
putative [Ricinus communis]
Length = 505
Score = 626 bits (1614), Expect = e-177
Identities = 305/506 (60%), Positives = 387/506 (76%)
Frame = +3
Query: 120 MALIWKTILVVIAITALLDRFLKKIIKKRRVPLPPSXXXXXXXXXXXXXXXNPHQDLHKL 299
M+L W T L +IA+ L L + K LPP PH++LH+L
Sbjct: 9 MSLTWAT-LALIALACFLQALLW-LRKTNNKKLPPGPRGFPIFGSLHLLGKYPHRELHRL 66
Query: 300 SKEHGPIMYMKFGMIPNIIVSSPDAAEQFLKTHDLVFASRPPHEAAKYISYDQTNLSFAP 479
++++GPIM+++ G++ I+VSSP AAE FLKTHDL FASRPPH+AAK+ISY+Q NLSFAP
Sbjct: 67 AQKYGPIMHLRLGLVSTIVVSSPQAAESFLKTHDLAFASRPPHQAAKFISYEQKNLSFAP 126
Query: 480 YGHYWRNMRKLCTLELLSNMKINSFHPMRSEEISNFIDLLKSCSQDRSVVDLSSQVSTLN 659
YG YWRN+RK+CTLELLSN+K+NSF MR EE+ ID +K+ S R VDLS++V++L+
Sbjct: 127 YGSYWRNVRKMCTLELLSNVKVNSFKSMRKEELDLLIDCIKNASCQRVAVDLSAKVASLS 186
Query: 660 TNMTCRMIFGKKYTNEEFGDKKGFEGVIQEGMQLAAKPNLGDYFPYLGKLDLQGLTARFK 839
+M+CRM+FGKKY ++EF D++GF+ VIQEGM L A PNLG+Y P + LDLQGLT R K
Sbjct: 187 ADMSCRMVFGKKYMDKEF-DERGFKAVIQEGMHLGAAPNLGNYIPQIAGLDLQGLTKRMK 245
Query: 840 AVSAVFDAFFEKVLDEHEQQSSRGHENQEGKNMDFVDTMLGILTSGQAEFTFDRRHVKAV 1019
AVS VFD+FFEK++DEH + E + DFVD MLG + S +E+ R ++KA+
Sbjct: 246 AVSKVFDSFFEKIIDEHMEFKD------ENRTKDFVDVMLGFMGSEVSEYHIGRDNIKAI 299
Query: 1020 LLDMLAGAMDTSATVIDWTMSELLRHPTIMKNVQSELEQVVGLNRIVEESDLGKLEYLNM 1199
+LDMLAG+MDTSA VI+W +SELL+HP +MK VQ ELE+ VG+ R+VEESD+ KLEYL M
Sbjct: 300 ILDMLAGSMDTSAAVIEWALSELLKHPGVMKKVQKELEEKVGMTRMVEESDVEKLEYLEM 359
Query: 1200 VVKESMRLHPVAPLLIPHEALEDCTVEGYHIPKKSRVIINAWSIGRDPEAWSDPEKFIPK 1379
V+KE+ RLHPVAPLL+PHEA ED T++GY IPKKS +IIN ++IGRDP AW++ EKF+P+
Sbjct: 360 VIKETFRLHPVAPLLLPHEAAEDTTIDGYLIPKKSHIIINTFAIGRDPSAWTEAEKFLPE 419
Query: 1380 RFQGSKIDVRGRDFQLIPFGSGRRGCPGIQLGLIVVSLVVAQLVHCFDWELPNGMLNTEL 1559
RF G ID+RGRDFQL+PFG+GRRGCPGIQLG+ VV LVVAQLVHCFDWELPNGML TEL
Sbjct: 420 RFLGRNIDIRGRDFQLLPFGAGRRGCPGIQLGMTVVRLVVAQLVHCFDWELPNGMLPTEL 479
Query: 1560 DMSEEFGLVTTRAGHLMAIPTYRLNV 1637
DM+EEF LVT RA HL AIP YRL++
Sbjct: 480 DMTEEFSLVTPRAKHLEAIPNYRLHI 505
>ref|XP_002280620.1| PREDICTED: cytochrome P450 71A1 [Vitis vinifera]
Length = 498
Score = 622 bits (1603), Expect = e-175
Identities = 298/500 (59%), Positives = 384/500 (76%)
Frame = +3
Query: 132 WKTILVVIAITALLDRFLKKIIKKRRVPLPPSXXXXXXXXXXXXXXXNPHQDLHKLSKEH 311
W I + + A + R L I K + LPP PHQDL +L+K++
Sbjct: 3 WSWIALSLIALAYVVRALLNISKNKHKRLPPGPRGIPILGNLHMLGELPHQDLLRLAKKY 62
Query: 312 GPIMYMKFGMIPNIIVSSPDAAEQFLKTHDLVFASRPPHEAAKYISYDQTNLSFAPYGHY 491
GPIMYM+F ++P I+VSSP AAEQFLKT+DLVFA RPPHE ++ +SYD+ +SF YG Y
Sbjct: 63 GPIMYMRFALVPTIVVSSPQAAEQFLKTNDLVFAGRPPHEGSRIVSYDRKGISFTDYGPY 122
Query: 492 WRNMRKLCTLELLSNMKINSFHPMRSEEISNFIDLLKSCSQDRSVVDLSSQVSTLNTNMT 671
WRNMRKLCTL LLSN++I+SF P+R EE+ I LK + R+ VDLS+++S+L+ +M+
Sbjct: 123 WRNMRKLCTLGLLSNLRISSFQPLRREELDLLIKSLKEAALARTAVDLSAKISSLSADMS 182
Query: 672 CRMIFGKKYTNEEFGDKKGFEGVIQEGMQLAAKPNLGDYFPYLGKLDLQGLTARFKAVSA 851
CRMIFGKKY +++ D++GF+ VIQEGMQLA PN+GDY P++ LDLQGL R KA+S
Sbjct: 183 CRMIFGKKYMDKDI-DERGFKAVIQEGMQLAGAPNIGDYIPFVAPLDLQGLARRMKAISK 241
Query: 852 VFDAFFEKVLDEHEQQSSRGHENQEGKNMDFVDTMLGILTSGQAEFTFDRRHVKAVLLDM 1031
VFDAFFEK++D+H + +EG+ D +D MLG + S + EF +R ++KA++LDM
Sbjct: 242 VFDAFFEKIIDDHIHEPK-----EEGQPKDLIDVMLGYMGSKENEFQIERSNIKALVLDM 296
Query: 1032 LAGAMDTSATVIDWTMSELLRHPTIMKNVQSELEQVVGLNRIVEESDLGKLEYLNMVVKE 1211
LAG+MDTSAT I+W ++ELL++P IMK VQ ELE+VVG+ R VEESDL LEYL+MVVKE
Sbjct: 297 LAGSMDTSATAIEWALAELLKNPRIMKKVQEELEKVVGMERKVEESDLESLEYLDMVVKE 356
Query: 1212 SMRLHPVAPLLIPHEALEDCTVEGYHIPKKSRVIINAWSIGRDPEAWSDPEKFIPKRFQG 1391
++RLHPVAPLLIPHE+LEDCTV G+HIP+KSRV++N ++IGRDP W+D EKF+P+RF G
Sbjct: 357 TLRLHPVAPLLIPHESLEDCTVNGFHIPQKSRVMVNTYAIGRDPNVWTDAEKFLPERFIG 416
Query: 1392 SKIDVRGRDFQLIPFGSGRRGCPGIQLGLIVVSLVVAQLVHCFDWELPNGMLNTELDMSE 1571
S ID+RGRDFQLIPFGSGRRGCPG+QLGL VV LV+AQLVHCFDWELPNGM+ +ELDM+E
Sbjct: 417 SSIDLRGRDFQLIPFGSGRRGCPGMQLGLTVVRLVLAQLVHCFDWELPNGMMPSELDMTE 476
Query: 1572 EFGLVTTRAGHLMAIPTYRL 1631
EFGL RA H++A+PTYRL
Sbjct: 477 EFGLTVPRAKHILAVPTYRL 496
>ref|XP_003524281.1| PREDICTED: cytochrome P450 71A1-like [Glycine max]
Length = 536
Score = 619 bits (1596), Expect = e-175
Identities = 307/524 (58%), Positives = 390/524 (74%)
Frame = +3
Query: 63 MHDHFLSP*K*IITTNLITMALIWKTILVVIAITALLDRFLKKIIKKRRVPLPPSXXXXX 242
+H+ F+ K I T+L +IW + I + +L +L++I K + LPP
Sbjct: 21 LHNCFILAAK--IYTHLAIATMIW----IAIFLVSLAYMWLRRISKNKAKKLPPGPRGLP 74
Query: 243 XXXXXXXXXXNPHQDLHKLSKEHGPIMYMKFGMIPNIIVSSPDAAEQFLKTHDLVFASRP 422
NPH+DLH+L++++GP+M+++ G +P I+VSSP AAE FLKTHDLVFASRP
Sbjct: 75 ILGSLHKLGPNPHRDLHQLAQKYGPVMHLRLGFVPTIVVSSPQAAELFLKTHDLVFASRP 134
Query: 423 PHEAAKYISYDQTNLSFAPYGHYWRNMRKLCTLELLSNMKINSFHPMRSEEISNFIDLLK 602
P EAAKYIS++Q NLSFA YG YWRN+RK+CTLELLS+ KINSF MR EE+ + LL+
Sbjct: 135 PLEAAKYISWEQRNLSFAEYGSYWRNVRKMCTLELLSHTKINSFRSMREEELDLMVKLLR 194
Query: 603 SCSQDRSVVDLSSQVSTLNTNMTCRMIFGKKYTNEEFGDKKGFEGVIQEGMQLAAKPNLG 782
++D +VVDLS++VSTL+ +M+CRM+ GKKY + + D+KGF+ V+QEGM LAA PN+G
Sbjct: 195 EAAKDGAVVDLSAKVSTLSADMSCRMVLGKKYMDRDL-DEKGFKAVMQEGMHLAATPNMG 253
Query: 783 DYFPYLGKLDLQGLTARFKAVSAVFDAFFEKVLDEHEQQSSRGHENQEGKNMDFVDTMLG 962
DY PY+ LDLQGLT R K V +FD FFEK++DEH Q E E + DFVD ML
Sbjct: 254 DYIPYIAALDLQGLTKRMKVVGKIFDDFFEKIIDEHLQS-----EKGEDRTKDFVDVMLD 308
Query: 963 ILTSGQAEFTFDRRHVKAVLLDMLAGAMDTSATVIDWTMSELLRHPTIMKNVQSELEQVV 1142
+ + ++E+ +R ++KA+LLDMLAG+MDTSAT I+WT+SELL++P +MK VQ ELE VV
Sbjct: 309 FVGTEESEYRIERPNIKAILLDMLAGSMDTSATAIEWTLSELLKNPRVMKKVQMELETVV 368
Query: 1143 GLNRIVEESDLGKLEYLNMVVKESMRLHPVAPLLIPHEALEDCTVEGYHIPKKSRVIINA 1322
G+ R VEESDL KL YL+MVVKESMRLHPVAPLLIPH++ EDC V IPKKSRVI+NA
Sbjct: 369 GMKRKVEESDLDKLVYLDMVVKESMRLHPVAPLLIPHQSTEDCMVGDLFIPKKSRVIVNA 428
Query: 1323 WSIGRDPEAWSDPEKFIPKRFQGSKIDVRGRDFQLIPFGSGRRGCPGIQLGLIVVSLVVA 1502
W+I RDP AW + EKF P+RF+GS IDVRGRDF+LIPFGSGRRGCPG+QLGL VV L VA
Sbjct: 429 WAIMRDPSAWDEAEKFWPERFEGSSIDVRGRDFELIPFGSGRRGCPGLQLGLTVVRLTVA 488
Query: 1503 QLVHCFDWELPNGMLNTELDMSEEFGLVTTRAGHLMAIPTYRLN 1634
Q+VHCFDW+LP +L +LDM EEFGL RA HL AIPTYRL+
Sbjct: 489 QIVHCFDWKLPKDILPDDLDMKEEFGLTMPRANHLHAIPTYRLS 532
>ref|XP_002310008.1| cytochrome P450 [Populus trichocarpa] gi|222852911|gb|EEE90458.1|
cytochrome P450 [Populus trichocarpa]
Length = 493
Score = 607 bits (1566), Expect = e-171
Identities = 293/505 (58%), Positives = 381/505 (75%)
Frame = +3
Query: 120 MALIWKTILVVIAITALLDRFLKKIIKKRRVPLPPSXXXXXXXXXXXXXXXNPHQDLHKL 299
MA IW ++ V I FL + + + LPP PH+ LH+L
Sbjct: 1 MAWIWTSLAFVALI------FLLQWLSTKNKRLPPGPRGFPIFGSLHLLGKFPHRALHQL 54
Query: 300 SKEHGPIMYMKFGMIPNIIVSSPDAAEQFLKTHDLVFASRPPHEAAKYISYDQTNLSFAP 479
++++GPIM+++ G++P I+VSSP+AAE FLKTHDLVFA RPPHE+A+YISY Q ++FA
Sbjct: 55 AQKYGPIMHLRLGLVPTIVVSSPEAAELFLKTHDLVFAGRPPHESARYISYGQKGMAFAQ 114
Query: 480 YGHYWRNMRKLCTLELLSNMKINSFHPMRSEEISNFIDLLKSCSQDRSVVDLSSQVSTLN 659
YG YWRN+RK+CT+ELLS++KI SF PMR EE+ I ++ +Q+R VDLS++VS+L+
Sbjct: 115 YGSYWRNIRKMCTVELLSSLKITSFKPMRMEELDLLIKYIQEAAQERVAVDLSAKVSSLS 174
Query: 660 TNMTCRMIFGKKYTNEEFGDKKGFEGVIQEGMQLAAKPNLGDYFPYLGKLDLQGLTARFK 839
+M+CRM+FGKKY +E+ D++GF+ V+QE M L+A PNLGDY P +G LDLQGLT R K
Sbjct: 175 ADMSCRMVFGKKYLDEDL-DERGFKSVMQEVMHLSAAPNLGDYIPQIGALDLQGLTKRMK 233
Query: 840 AVSAVFDAFFEKVLDEHEQQSSRGHENQEGKNMDFVDTMLGILTSGQAEFTFDRRHVKAV 1019
A+S V D F K++DEH Q ++GKN DFVD ML + S + E+ D+ +KA+
Sbjct: 234 AISKVLDLFVSKIIDEHAQYQ------EKGKNKDFVDVMLSCMKSEENEYLVDQGCMKAI 287
Query: 1020 LLDMLAGAMDTSATVIDWTMSELLRHPTIMKNVQSELEQVVGLNRIVEESDLGKLEYLNM 1199
+LDML G+MDTSATVIDW SEL+++P +MK +Q ELE+VVG R+VEESDL +LEYL+M
Sbjct: 288 MLDMLVGSMDTSATVIDWAFSELIKNPRVMKKLQKELEEVVGKQRMVEESDLERLEYLDM 347
Query: 1200 VVKESMRLHPVAPLLIPHEALEDCTVEGYHIPKKSRVIINAWSIGRDPEAWSDPEKFIPK 1379
VVKE++RLHP PL+IPHEA EDC V +HIPKKS VIIN W+IGRDP+AW+D EKF P+
Sbjct: 348 VVKETLRLHPAGPLMIPHEATEDCVVNDFHIPKKSHVIINVWAIGRDPKAWTDAEKFYPE 407
Query: 1380 RFQGSKIDVRGRDFQLIPFGSGRRGCPGIQLGLIVVSLVVAQLVHCFDWELPNGMLNTEL 1559
RF GS IDVRGRDFQLIPFG+GRR CPG+QLGL +V LV+AQ+VHCFDWELPNG+L +E+
Sbjct: 408 RFVGSDIDVRGRDFQLIPFGTGRRSCPGMQLGLTMVRLVLAQMVHCFDWELPNGILPSEV 467
Query: 1560 DMSEEFGLVTTRAGHLMAIPTYRLN 1634
DMSEEFGLV R+ HL++IPTYRLN
Sbjct: 468 DMSEEFGLVLCRSKHLVSIPTYRLN 492