BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum22_contig00003456
         (1485 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ABC69422.1| CYP72A58 [Nicotiana tabacum]                           385   e-130
gb|ABC69414.1| CYP72A57 [Nicotiana tabacum]                           385   e-128
gb|ABC69417.1| CYP72A54 [Nicotiana tabacum]                           373   e-128
gb|AAZ07706.1| cytochrome P450 monooxygenase [Sesamum indicum]        365   e-127
ref|XP_002265455.1| PREDICTED: secologanin synthase isoform 1 [V...   353   e-122

>gb|ABC69422.1| CYP72A58 [Nicotiana tabacum]
          Length = 513

 Score =  385 bits (989), Expect(2) = e-130
 Identities = 186/310 (60%), Positives = 228/310 (73%)
 Frame = +3

Query: 6   SKPIGLSDDIVPRVFPFFLDLVKNHGKSCFAWFGPKPGXXXXXXXXXXXXXXKHYEFHKV 185
           SKP+ LSDD+ PR+ PFFL+ +K +GK  F WFGP+P               K + + K 
Sbjct: 65  SKPMSLSDDVAPRLMPFFLETIKKYGKRSFIWFGPRPLVLIMDPELIKEVLSKIHLYQKP 124

Query: 186 KSNPLTKLLAQGIASYEEDKWAKHRKLINPAFHVEKLKHMLPAFELCCTEMLSKWEENMG 365
             NPL  LL QGIA+YEEDKWAKHRK+INPAFH+EKLK MLPAF L C+EMLSKWE+ + 
Sbjct: 125 GGNPLATLLVQGIATYEEDKWAKHRKIINPAFHLEKLKLMLPAFRLSCSEMLSKWEDIVS 184

Query: 366 SQGSSEVDIWPYLQAMTSDVISRTAFGSSYEEGAKIFELQRELAVHVLNIARSLYIPGWR 545
           +  S E+D+W +L+ +T DVISRTAFGSSYEEG KIFELQ+E A +++ + RS+YIPG R
Sbjct: 185 ADSSHEIDVWSHLEQLTCDVISRTAFGSSYEEGRKIFELQKEQAQYLVEVFRSVYIPGRR 244

Query: 546 FIPTKRNRRMKEIDKQXXXXXXXXXXXXXXXXXXGEVKSDDLLGILLESNFKGIEQHGNK 725
           F+PTKRNRRMKEI K                   G+  ++DLLGILLESN K IEQHGNK
Sbjct: 245 FLPTKRNRRMKEIKKDVRASIKGIIDKRLKAMKAGDTNNEDLLGILLESNIKEIEQHGNK 304

Query: 726 DYGMSINEIIEECKLFYFAGQETTSVVLLWAMVLLSSHQDWQERARKEVLELFADGKPDS 905
           D+GMSI E+IEECKLFYFAGQETTSV+LLW++VLLS +QDWQ RAR+E+L++F   KPD 
Sbjct: 305 DFGMSIEEVIEECKLFYFAGQETTSVLLLWSLVLLSRYQDWQARAREEILQVFGSRKPDF 364

Query: 906 DDLNRLKIVS 935
           D LN LKIV+
Sbjct: 365 DGLNHLKIVT 374



 Score =  106 bits (265), Expect(2) = e-130
 Identities = 50/89 (56%), Positives = 62/89 (69%)
 Frame = +2

Query: 1007 HRSQVTMIFHEVMRLYPPLMELM*GIPKETRLGNITLPKGVRVFLPTILLHHDKEMWGAD 1186
            H   VTMI +E +RLYP L+ L     ++  LG ++LP GV V LP ILLHHD+E+WG D
Sbjct: 369  HLKIVTMILYESLRLYPSLITLTRRCNEDIVLGELSLPAGVLVSLPLILLHHDEEIWGED 428

Query: 1187 VNDFKP*RFAEGVSKATKGQVCYFPFGWG 1273
              +FKP RF EG+S ATKGQ+ YFPF WG
Sbjct: 429  AKEFKPERFREGISSATKGQLTYFPFSWG 457



 Score = 70.5 bits (171), Expect = 1e-09
 Identities = 28/42 (66%), Positives = 34/42 (80%)
 Frame = +3

Query: 1311 ILLHHDKEMWGADVNDFKP*RFAEGVSKATKGKVCYFPFGWG 1436
            ILLHHD+E+WG D  +FKP RF EG+S ATKG++ YFPF WG
Sbjct: 416  ILLHHDEEIWGEDAKEFKPERFREGISSATKGQLTYFPFSWG 457


>gb|ABC69414.1| CYP72A57 [Nicotiana tabacum]
          Length = 518

 Score =  385 bits (988), Expect(2) = e-128
 Identities = 182/310 (58%), Positives = 226/310 (72%)
 Frame = +3

Query: 6   SKPIGLSDDIVPRVFPFFLDLVKNHGKSCFAWFGPKPGXXXXXXXXXXXXXXKHYEFHKV 185
           SKP+ LSDDI PR+ PFFLD +K +GK  F W GPKP               K+Y + K 
Sbjct: 70  SKPMNLSDDIAPRLVPFFLDTIKKYGKKSFVWLGPKPLVLIMDPELIKEIFSKYYLYQKP 129

Query: 186 KSNPLTKLLAQGIASYEEDKWAKHRKLINPAFHVEKLKHMLPAFELCCTEMLSKWEENMG 365
             NP+TKLL QG+ S EEDKWAKHRK+INPAFH+EKLKHMLPAF L CTEML KWE+ + 
Sbjct: 130 HGNPVTKLLVQGLVSLEEDKWAKHRKIINPAFHLEKLKHMLPAFCLSCTEMLCKWEDIVS 189

Query: 366 SQGSSEVDIWPYLQAMTSDVISRTAFGSSYEEGAKIFELQRELAVHVLNIARSLYIPGWR 545
            +GS E+D+WP+L+ ++SDVISRTAFGS++EEG +IFELQ+E A + +   RS+YIPGWR
Sbjct: 190 IKGSHEIDVWPHLEQLSSDVISRTAFGSNFEEGKRIFELQKEQAQYFVEAIRSVYIPGWR 249

Query: 546 FIPTKRNRRMKEIDKQXXXXXXXXXXXXXXXXXXGEVKSDDLLGILLESNFKGIEQHGNK 725
           F+PTKRNRRMKE++K                   GE  ++DLLGILLESNF   EQH +K
Sbjct: 250 FLPTKRNRRMKEVEKDVRASIRGIIDKRVKAMKAGEASNEDLLGILLESNFTEAEQHRHK 309

Query: 726 DYGMSINEIIEECKLFYFAGQETTSVVLLWAMVLLSSHQDWQERARKEVLELFADGKPDS 905
           D  MSI E+I+ECKLFY AGQETTSV+L+W ++LLS HQDWQ RAR+EV ++F + KPD 
Sbjct: 310 DSAMSIEEVIQECKLFYVAGQETTSVLLVWTLILLSRHQDWQSRAREEVFQVFGNQKPDF 369

Query: 906 DDLNRLKIVS 935
           D LNRLK+V+
Sbjct: 370 DGLNRLKVVT 379



 Score =  102 bits (255), Expect(2) = e-128
 Identities = 47/85 (55%), Positives = 62/85 (72%)
 Frame = +2

Query: 1019 VTMIFHEVMRLYPPLMELM*GIPKETRLGNITLPKGVRVFLPTILLHHDKEMWGADVNDF 1198
            VTMI +E +RLY P++ L+    ++  LGN++LP+GV + LP ILLHHD+E+WG D   F
Sbjct: 378  VTMILYESLRLYSPVVSLIRRPNEDAILGNVSLPEGVLLSLPVILLHHDEEIWGKDAKKF 437

Query: 1199 KP*RFAEGVSKATKGQVCYFPFGWG 1273
             P RF +GVS ATKGQV +FPF WG
Sbjct: 438  NPERFRDGVSSATKGQVTFFPFTWG 462



 Score = 65.5 bits (158), Expect = 4e-08
 Identities = 27/42 (64%), Positives = 32/42 (76%)
 Frame = +3

Query: 1311 ILLHHDKEMWGADVNDFKP*RFAEGVSKATKGKVCYFPFGWG 1436
            ILLHHD+E+WG D   F P RF +GVS ATKG+V +FPF WG
Sbjct: 421  ILLHHDEEIWGKDAKKFNPERFRDGVSSATKGQVTFFPFTWG 462


>gb|ABC69417.1| CYP72A54 [Nicotiana tabacum]
          Length = 517

 Score =  373 bits (957), Expect(2) = e-128
 Identities = 176/311 (56%), Positives = 226/311 (72%)
 Frame = +3

Query: 3   KSKPIGLSDDIVPRVFPFFLDLVKNHGKSCFAWFGPKPGXXXXXXXXXXXXXXKHYEFHK 182
           K+KP+  ++D V RV P F  L+  +GK+ F W GPKP               K Y + +
Sbjct: 69  KTKPMNFTNDYVARVLPHFTKLMLQYGKNSFMWLGPKPTMFITDPELIREILSKSYIYQE 128

Query: 183 VKSNPLTKLLAQGIASYEEDKWAKHRKLINPAFHVEKLKHMLPAFELCCTEMLSKWEENM 362
           ++ NP+TKLLAQG+ SYE +KWAKHRK+INPAFH++KLKHMLP+F L C +ML KWE   
Sbjct: 129 IQGNPITKLLAQGLVSYEAEKWAKHRKIINPAFHLDKLKHMLPSFYLSCCDMLRKWESIA 188

Query: 363 GSQGSSEVDIWPYLQAMTSDVISRTAFGSSYEEGAKIFELQRELAVHVLNIARSLYIPGW 542
            S+GS E+D+WP+L+ +TSD ISRTAFGS+YE+G +IFELQ+E A  +L  AR LYIPGW
Sbjct: 189 SSEGS-EIDVWPFLETLTSDAISRTAFGSNYEDGRQIFELQKEQAELILQAARWLYIPGW 247

Query: 543 RFIPTKRNRRMKEIDKQXXXXXXXXXXXXXXXXXXGEVKSDDLLGILLESNFKGIEQHGN 722
           RF+PTKRN+RMK+I K+                  GE   DDLLGILLESNFK I+ HGN
Sbjct: 248 RFVPTKRNKRMKQIAKEVRSLVLGIINKRIREMKAGEAAKDDLLGILLESNFKEIQMHGN 307

Query: 723 KDYGMSINEIIEECKLFYFAGQETTSVVLLWAMVLLSSHQDWQERARKEVLELFADGKPD 902
           K++GM+I+E+IEECKLFYFAGQETTSV+L+W ++LLS H DWQERAR+EV ++F   KPD
Sbjct: 308 KNFGMTIDEVIEECKLFYFAGQETTSVLLVWTLILLSKHVDWQERAREEVHQVFGSNKPD 367

Query: 903 SDDLNRLKIVS 935
            D LN+LK+V+
Sbjct: 368 YDALNQLKVVT 378



 Score =  114 bits (285), Expect(2) = e-128
 Identities = 49/85 (57%), Positives = 67/85 (78%)
 Frame = +2

Query: 1019 VTMIFHEVMRLYPPLMELM*GIPKETRLGNITLPKGVRVFLPTILLHHDKEMWGADVNDF 1198
            VTMIF+EV+RLYPP + +   + ++T+LGN++LP G+++ LP I LHHD E+WG D  +F
Sbjct: 377  VTMIFNEVLRLYPPGITISRTVHEDTKLGNLSLPAGIQLVLPAIWLHHDNEIWGDDAKEF 436

Query: 1199 KP*RFAEGVSKATKGQVCYFPFGWG 1273
            KP RF+EGV+KATKG+  YFPF WG
Sbjct: 437  KPERFSEGVNKATKGKFAYFPFSWG 461



 Score = 70.5 bits (171), Expect = 1e-09
 Identities = 29/42 (69%), Positives = 33/42 (78%)
 Frame = +3

Query: 1311 ILLHHDKEMWGADVNDFKP*RFAEGVSKATKGKVCYFPFGWG 1436
            I LHHD E+WG D  +FKP RF+EGV+KATKGK  YFPF WG
Sbjct: 420  IWLHHDNEIWGDDAKEFKPERFSEGVNKATKGKFAYFPFSWG 461


>gb|AAZ07706.1| cytochrome P450 monooxygenase [Sesamum indicum]
          Length = 514

 Score =  365 bits (937), Expect(2) = e-127
 Identities = 179/311 (57%), Positives = 219/311 (70%)
 Frame = +3

Query: 3   KSKPIGLSDDIVPRVFPFFLDLVKNHGKSCFAWFGPKPGXXXXXXXXXXXXXXKHYEFHK 182
           KSKP+  S +IVPRV P  +  VKN+G+  F WFGP+P               K Y F  
Sbjct: 66  KSKPMSFSHEIVPRVAPSIIKTVKNYGEQSFLWFGPRPAVIVLDPEMVREILSKSYVFQM 125

Query: 183 VKSNPLTKLLAQGIASYEEDKWAKHRKLINPAFHVEKLKHMLPAFELCCTEMLSKWEENM 362
              NP +++LA+GIASYE DKWAKHR+LINPAFHVEKLKHM+PAF L C +MLSKWE+ +
Sbjct: 126 PGGNPTSRMLARGIASYETDKWAKHRRLINPAFHVEKLKHMVPAFYLSCADMLSKWEKIV 185

Query: 363 GSQGSSEVDIWPYLQAMTSDVISRTAFGSSYEEGAKIFELQRELAVHVLNIARSLYIPGW 542
             +G  E+D+WPYLQ +TSD ISRTAFGSSYEEG +IFELQ+E A  ++   R++ IPG 
Sbjct: 186 PREGLCELDVWPYLQTLTSDAISRTAFGSSYEEGRRIFELQKEQAELIMEALRTVCIPGS 245

Query: 543 RFIPTKRNRRMKEIDKQXXXXXXXXXXXXXXXXXXGEVKSDDLLGILLESNFKGIEQHGN 722
           RF+PTK N+RM +I ++                  GE   DDLLG+LLESN K IEQHGN
Sbjct: 246 RFLPTKNNKRMNKIVREVESSVLGIINKRMKAIKAGETAGDDLLGLLLESNLKEIEQHGN 305

Query: 723 KDYGMSINEIIEECKLFYFAGQETTSVVLLWAMVLLSSHQDWQERARKEVLELFADGKPD 902
           K +GMS+ E+IEECKLFYFAGQETTS +L+W M+LLS H+DWQERAR EVL+LF   KPD
Sbjct: 306 K-FGMSLKEVIEECKLFYFAGQETTSTLLVWTMILLSKHKDWQERARDEVLQLFGRDKPD 364

Query: 903 SDDLNRLKIVS 935
             +LN LKIVS
Sbjct: 365 YQELNHLKIVS 375



 Score =  118 bits (296), Expect(2) = e-127
 Identities = 53/89 (59%), Positives = 69/89 (77%)
 Frame = +2

Query: 1007 HRSQVTMIFHEVMRLYPPLMELM*GIPKETRLGNITLPKGVRVFLPTILLHHDKEMWGAD 1186
            H   V+MIFHEV+RLYPP + L     KE+ LG +TLP GV++ +P +LLHHD+++WG D
Sbjct: 370  HLKIVSMIFHEVLRLYPPGVMLNRISMKESTLGKVTLPAGVQLLMPAVLLHHDRKIWGDD 429

Query: 1187 VNDFKP*RFAEGVSKATKGQVCYFPFGWG 1273
              +FKP RF+EGVSKAT+GQ+ YFPFGWG
Sbjct: 430  AEEFKPERFSEGVSKATQGQLVYFPFGWG 458



 Score = 72.4 bits (176), Expect = 3e-10
 Identities = 28/42 (66%), Positives = 37/42 (88%)
 Frame = +3

Query: 1311 ILLHHDKEMWGADVNDFKP*RFAEGVSKATKGKVCYFPFGWG 1436
            +LLHHD+++WG D  +FKP RF+EGVSKAT+G++ YFPFGWG
Sbjct: 417  VLLHHDRKIWGDDAEEFKPERFSEGVSKATQGQLVYFPFGWG 458


>ref|XP_002265455.1| PREDICTED: secologanin synthase isoform 1 [Vitis vinifera]
          Length = 515

 Score =  353 bits (906), Expect(2) = e-122
 Identities = 168/311 (54%), Positives = 219/311 (70%), Gaps = 1/311 (0%)
 Frame = +3

Query: 6   SKPIGLSDDIVPRVFPFFLDLVKNHGKSCFAWFGPKPGXXXXXXXXXXXXXXKHYEFHKV 185
           S+PI LSDDIV RV PF    +K +GK+CF W GPKP               KH  F K 
Sbjct: 66  SRPISLSDDIVQRVLPFHYHSIKKYGKNCFIWMGPKPVVNIMEPELIRDVLLKHNAFQKP 125

Query: 186 KSNPLTKLLAQGIASYEEDKWAKHRKLINPAFHVEKLKHMLPAFELCCTEMLSKWEENMG 365
             +PL KLLA G+ + E ++W K RK+INPAFH+EKLKHM+PAF+L C+EM++KWE+ + 
Sbjct: 126 PVHPLGKLLATGVIALEGEQWTKRRKIINPAFHLEKLKHMVPAFQLSCSEMVNKWEKKLS 185

Query: 366 SQGSSEVDIWPYLQAMTSDVISRTAFGSSYEEGAKIFELQRELAVHVLNIARSLYIPGWR 545
             GS E+DIWP L+ +  DVISRTAFGSSYEEG +IF+LQ+E A   + +++S+YIPGWR
Sbjct: 186 KDGSCELDIWPDLENLAGDVISRTAFGSSYEEGRRIFQLQKEQAHLAVQVSQSIYIPGWR 245

Query: 546 FIPTKRNRRMKEIDKQXXXXXXXXXXXXXXXXXXGEVKSDDLLGILLESNFKGIEQHG-N 722
           F+PTK N+RM++I  +                  GE  +DDLLG+L+ESN+K +++HG  
Sbjct: 246 FVPTKTNKRMRQISNEVNALLKGIIERREKAMKVGETANDDLLGLLMESNYKEMQEHGER 305

Query: 723 KDYGMSINEIIEECKLFYFAGQETTSVVLLWAMVLLSSHQDWQERARKEVLELFADGKPD 902
           K+ GMS  ++IEECKLFYFAGQETTSV+LLW MVLLS H +WQ RAR+EVL++F + KPD
Sbjct: 306 KNVGMSNKDVIEECKLFYFAGQETTSVLLLWTMVLLSKHSNWQARAREEVLQVFGNKKPD 365

Query: 903 SDDLNRLKIVS 935
            D LN LKIV+
Sbjct: 366 GDGLNHLKIVT 376



 Score =  115 bits (287), Expect(2) = e-122
 Identities = 53/89 (59%), Positives = 65/89 (73%)
 Frame = +2

Query: 1007 HRSQVTMIFHEVMRLYPPLMELM*GIPKETRLGNITLPKGVRVFLPTILLHHDKEMWGAD 1186
            H   VTMIFHEV+RLYPP   L+  +  +T +G + LP GV+V LP +LLHHD E+WG D
Sbjct: 371  HLKIVTMIFHEVLRLYPPASMLIRSVYADTEVGGMYLPDGVQVSLPILLLHHDHEIWGDD 430

Query: 1187 VNDFKP*RFAEGVSKATKGQVCYFPFGWG 1273
              DF P RF+EGVSKATKGQ  +FPFG+G
Sbjct: 431  AKDFNPERFSEGVSKATKGQFAFFPFGYG 459



 Score = 68.2 bits (165), Expect = 5e-09
 Identities = 28/42 (66%), Positives = 34/42 (80%)
 Frame = +3

Query: 1311 ILLHHDKEMWGADVNDFKP*RFAEGVSKATKGKVCYFPFGWG 1436
            +LLHHD E+WG D  DF P RF+EGVSKATKG+  +FPFG+G
Sbjct: 418  LLLHHDHEIWGDDAKDFNPERFSEGVSKATKGQFAFFPFGYG 459