BLASTX nr result
ID: Lithospermum22_contig00002649
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Lithospermum22_contig00002649
(1674 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
emb|CBI34714.3| unnamed protein product [Vitis vinifera] 518 e-144
ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3... 518 e-144
emb|CBI34715.3| unnamed protein product [Vitis vinifera] 517 e-144
ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxy... 516 e-144
ref|XP_002298058.1| cytochrome P450 [Populus trichocarpa] gi|222... 506 e-141
>emb|CBI34714.3| unnamed protein product [Vitis vinifera]
Length = 936
Score = 518 bits (1333), Expect = e-144
Identities = 239/452 (52%), Positives = 338/452 (74%)
Frame = +3
Query: 156 LVGNIPHMRNMTHISLANLAQIHGPIMSLKLPNKYIIVGSSKEVAIEILKRHDHLLSGRD 335
++GN+ H+ NM HISLA +Q +GP++SL+L ++ ++V S+ A+EILK HD +LSGR
Sbjct: 486 ILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAAMEILKTHDRVLSGRY 545
Query: 336 PPIGHPYTKEELNHISIGWPEECNERWKYLRALCKAELFSGKALASQEYVRQKKTFEMVE 515
P P E+N +S+GW ECN+ WK LR +C+AELFS K + SQ +V +KK EMV
Sbjct: 546 VPHAVPAKNSEINRMSLGWAVECNDAWKNLRTVCRAELFSTKVMESQAWVGEKKVMEMVR 605
Query: 516 LIKASEGKVVQVRQIIFATIFNMLSSILLSRDLVSLEEEVEDRKMKGLMRRVTEILMEPN 695
+ EG+V++V +++FAT+ N L+++L+SRD +S E++ +D MKGL+R++ + PN
Sbjct: 606 FVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDYKDGGMKGLVRKMMMAMAAPN 665
Query: 696 ISDIFPALNSLDLQGVHKKGKKLWKEIWDIFEPLVEEKRLRKSKENAEKQDFLDTLVALQ 875
+ D + + LDLQG++KK K+L I ++E ++ E+R + ++ KQDFL+ L+
Sbjct: 666 LDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERR-EGASDDPSKQDFLNILIRSG 724
Query: 876 FSDDEVQELLVELFSAGSDTSTSTIEWAMAELVKNPEAMKNLQDELEREISQDLIKETHL 1055
+SDD++ +L +EL +AG+DTS+ST+EWAMAEL+K+PE+MK + +EL REIS +L K + L
Sbjct: 725 YSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREISDNLPKASDL 784
Query: 1056 MQLPYLQACVKETLRLHPAAPLLIPRKPIEACRVMNYTIPKNSQVLVNVWAIGRDQTTWE 1235
LPYLQACVKETLRLHP+APLL+PR+ +C VMNYTIPK+SQ+ VN WAIGRD WE
Sbjct: 785 PHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWE 844
Query: 1236 DPMEFKPERFLNSSLDFKGNDFEFLPFGGGRRICPGYPMAMRQIPLAIASMIHYFNWYLP 1415
DP+ FKPERFLNS++DFKGN+ EF+PFG GRRICPG PMA R +PL +AS+ H+F+W LP
Sbjct: 845 DPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLP 904
Query: 1416 KGTTPDMLDMSESFGLTLEKEHPLLIIPRARR 1511
GTTPD LDM++ FG+ L+KE PLLIIP+ R+
Sbjct: 905 NGTTPDELDMNDKFGIALQKEQPLLIIPKVRK 936
>ref|XP_002283461.1| PREDICTED: probable (S)-N-methylcoclaurine 3'-hydroxylase isozyme
2-like [Vitis vinifera]
Length = 503
Score = 518 bits (1333), Expect = e-144
Identities = 239/452 (52%), Positives = 338/452 (74%)
Frame = +3
Query: 156 LVGNIPHMRNMTHISLANLAQIHGPIMSLKLPNKYIIVGSSKEVAIEILKRHDHLLSGRD 335
++GN+ H+ NM HISLA +Q +GP++SL+L ++ ++V S+ A+EILK HD +LSGR
Sbjct: 53 ILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAAMEILKTHDRVLSGRY 112
Query: 336 PPIGHPYTKEELNHISIGWPEECNERWKYLRALCKAELFSGKALASQEYVRQKKTFEMVE 515
P P E+N +S+GW ECN+ WK LR +C+AELFS K + SQ +V +KK EMV
Sbjct: 113 VPHAVPAKNSEINRMSLGWAVECNDAWKNLRTVCRAELFSTKVMESQAWVGEKKVMEMVR 172
Query: 516 LIKASEGKVVQVRQIIFATIFNMLSSILLSRDLVSLEEEVEDRKMKGLMRRVTEILMEPN 695
+ EG+V++V +++FAT+ N L+++L+SRD +S E++ +D MKGL+R++ + PN
Sbjct: 173 FVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDYKDGGMKGLVRKMMMAMAAPN 232
Query: 696 ISDIFPALNSLDLQGVHKKGKKLWKEIWDIFEPLVEEKRLRKSKENAEKQDFLDTLVALQ 875
+ D + + LDLQG++KK K+L I ++E ++ E+R + ++ KQDFL+ L+
Sbjct: 233 LDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERR-EGASDDPSKQDFLNILIRSG 291
Query: 876 FSDDEVQELLVELFSAGSDTSTSTIEWAMAELVKNPEAMKNLQDELEREISQDLIKETHL 1055
+SDD++ +L +EL +AG+DTS+ST+EWAMAEL+K+PE+MK + +EL REIS +L K + L
Sbjct: 292 YSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREISDNLPKASDL 351
Query: 1056 MQLPYLQACVKETLRLHPAAPLLIPRKPIEACRVMNYTIPKNSQVLVNVWAIGRDQTTWE 1235
LPYLQACVKETLRLHP+APLL+PR+ +C VMNYTIPK+SQ+ VN WAIGRD WE
Sbjct: 352 PHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWE 411
Query: 1236 DPMEFKPERFLNSSLDFKGNDFEFLPFGGGRRICPGYPMAMRQIPLAIASMIHYFNWYLP 1415
DP+ FKPERFLNS++DFKGN+ EF+PFG GRRICPG PMA R +PL +AS+ H+F+W LP
Sbjct: 412 DPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLP 471
Query: 1416 KGTTPDMLDMSESFGLTLEKEHPLLIIPRARR 1511
GTTPD LDM++ FG+ L+KE PLLIIP+ R+
Sbjct: 472 NGTTPDELDMNDKFGIALQKEQPLLIIPKVRK 503
>emb|CBI34715.3| unnamed protein product [Vitis vinifera]
Length = 1076
Score = 517 bits (1332), Expect = e-144
Identities = 241/457 (52%), Positives = 340/457 (74%)
Frame = +3
Query: 156 LVGNIPHMRNMTHISLANLAQIHGPIMSLKLPNKYIIVGSSKEVAIEILKRHDHLLSGRD 335
++GN+ H+ NM HISLA +Q +GP++SL+L ++ ++V S+ A+EILK HD +LSGR
Sbjct: 617 ILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAAMEILKTHDRVLSGRY 676
Query: 336 PPIGHPYTKEELNHISIGWPEECNERWKYLRALCKAELFSGKALASQEYVRQKKTFEMVE 515
P P E+N +S+GW ECN WK LR +C+AELFS K + SQ +V +KK EMV
Sbjct: 677 VPHAVPAKNSEINPMSLGWAVECNGAWKNLRTVCRAELFSTKVMESQAWVGEKKVMEMVR 736
Query: 516 LIKASEGKVVQVRQIIFATIFNMLSSILLSRDLVSLEEEVEDRKMKGLMRRVTEILMEPN 695
+ EG+V++V +++FAT+ N L+++L+SRD +S E++ +D MKGL+R++ + PN
Sbjct: 737 FVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDNKDGGMKGLVRKMVMAMAAPN 796
Query: 696 ISDIFPALNSLDLQGVHKKGKKLWKEIWDIFEPLVEEKRLRKSKENAEKQDFLDTLVALQ 875
+ D + + LDLQG++KK K+L I ++E ++ E+R + ++ KQDFL+ L+
Sbjct: 797 LDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERR-EGASDDPSKQDFLNILIRSG 855
Query: 876 FSDDEVQELLVELFSAGSDTSTSTIEWAMAELVKNPEAMKNLQDELEREISQDLIKETHL 1055
+SDD++ +L +EL +AG+DTS+ST+EWAMAEL+K+PE+MK + +EL REIS +L K + L
Sbjct: 856 YSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREISDNLPKASDL 915
Query: 1056 MQLPYLQACVKETLRLHPAAPLLIPRKPIEACRVMNYTIPKNSQVLVNVWAIGRDQTTWE 1235
LPYLQACVKETLRLHP+APLL+PR+ +C VMNYTIPK+SQ+ VN WAIGRD WE
Sbjct: 916 PHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWE 975
Query: 1236 DPMEFKPERFLNSSLDFKGNDFEFLPFGGGRRICPGYPMAMRQIPLAIASMIHYFNWYLP 1415
DP+ FKPERFLNS++DFKGN+ EF+PFG GRRICPG PMA R +PL +AS+ H+F+W LP
Sbjct: 976 DPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLP 1035
Query: 1416 KGTTPDMLDMSESFGLTLEKEHPLLIIPRARR*SIVT 1526
GTTPD LDM++ FG+TL+KE PLLIIP+ R+ +T
Sbjct: 1036 NGTTPDELDMNDKFGVTLQKEQPLLIIPKKRKKKTIT 1072
>ref|XP_002283415.1| PREDICTED: (S)-N-methylcoclaurine 3'-hydroxylase isozyme 1-like
[Vitis vinifera]
Length = 503
Score = 516 bits (1330), Expect = e-144
Identities = 240/452 (53%), Positives = 338/452 (74%)
Frame = +3
Query: 156 LVGNIPHMRNMTHISLANLAQIHGPIMSLKLPNKYIIVGSSKEVAIEILKRHDHLLSGRD 335
++GN+ H+ NM HISLA +Q +GP++SL+L ++ ++V S+ A+EILK HD +LSGR
Sbjct: 53 ILGNLLHLGNMPHISLARFSQSYGPLISLRLGSQILVVASTSSAAMEILKTHDRVLSGRY 112
Query: 336 PPIGHPYTKEELNHISIGWPEECNERWKYLRALCKAELFSGKALASQEYVRQKKTFEMVE 515
P P E+N +S+GW ECN WK LR +C+AELFS K + SQ +V +KK EMV
Sbjct: 113 VPHAVPAKNSEINPMSLGWAVECNGAWKNLRTVCRAELFSTKVMESQAWVGEKKVMEMVR 172
Query: 516 LIKASEGKVVQVRQIIFATIFNMLSSILLSRDLVSLEEEVEDRKMKGLMRRVTEILMEPN 695
+ EG+V++V +++FAT+ N L+++L+SRD +S E++ +D MKGL+R++ + PN
Sbjct: 173 FVSTKEGEVMKVGEVVFATVLNTLTTVLMSRDFISFEDDNKDGGMKGLVRKMVMAMAAPN 232
Query: 696 ISDIFPALNSLDLQGVHKKGKKLWKEIWDIFEPLVEEKRLRKSKENAEKQDFLDTLVALQ 875
+ D + + LDLQG++KK K+L I ++E ++ E+R + ++ KQDFL+ L+
Sbjct: 233 LDDFYLIFSGLDLQGLNKKTKELIARICSMWESVIRERR-EGASDDPSKQDFLNILIRSG 291
Query: 876 FSDDEVQELLVELFSAGSDTSTSTIEWAMAELVKNPEAMKNLQDELEREISQDLIKETHL 1055
+SDD++ +L +EL +AG+DTS+ST+EWAMAEL+K+PE+MK + +EL REIS +L K + L
Sbjct: 292 YSDDQINQLFMELLTAGADTSSSTLEWAMAELIKSPESMKKVHEELAREISDNLPKASDL 351
Query: 1056 MQLPYLQACVKETLRLHPAAPLLIPRKPIEACRVMNYTIPKNSQVLVNVWAIGRDQTTWE 1235
LPYLQACVKETLRLHP+APLL+PR+ +C VMNYTIPK+SQ+ VN WAIGRD WE
Sbjct: 352 PHLPYLQACVKETLRLHPSAPLLLPRRASVSCEVMNYTIPKDSQIWVNAWAIGRDPMNWE 411
Query: 1236 DPMEFKPERFLNSSLDFKGNDFEFLPFGGGRRICPGYPMAMRQIPLAIASMIHYFNWYLP 1415
DP+ FKPERFLNS++DFKGN+ EF+PFG GRRICPG PMA R +PL +AS+ H+F+W LP
Sbjct: 412 DPLVFKPERFLNSAVDFKGNNLEFIPFGAGRRICPGLPMAARLLPLILASLTHFFDWSLP 471
Query: 1416 KGTTPDMLDMSESFGLTLEKEHPLLIIPRARR 1511
GTTPD LDM++ FG+TL+KE PLLIIP+ R+
Sbjct: 472 NGTTPDELDMNDKFGVTLQKEQPLLIIPKVRK 503
>ref|XP_002298058.1| cytochrome P450 [Populus trichocarpa] gi|222845316|gb|EEE82863.1|
cytochrome P450 [Populus trichocarpa]
Length = 507
Score = 506 bits (1303), Expect = e-141
Identities = 246/460 (53%), Positives = 333/460 (72%), Gaps = 8/460 (1%)
Frame = +3
Query: 156 LVGNIPHMRNMTHISLANLAQIHGPIMSLKLPNKYIIVGSSKEVAIEILKRHDHLLSGRD 335
++GNI + + HI+L + A+IHGPI SL+L + ++VGSS+ AI ILK HD +LSGR
Sbjct: 48 ILGNILQLGDKPHITLTHFAKIHGPIFSLRLGTQLVVVGSSQAAAIAILKTHDRILSGRH 107
Query: 336 PPIGHPYTKEELNHISIGWPEECNERWKYLRALCKAELFSGKALASQEYVRQKKTFEMVE 515
P P ELN +S+GW ECNERW+YLR +CK+ELFS KAL SQ +R++K EM+
Sbjct: 108 VPHMAPSKSSELNKLSLGWVVECNERWRYLRTICKSELFSLKALESQACIRERKAKEMIG 167
Query: 516 LIKASEGKVVQVRQIIFATIFNMLSSILLSRDLVSLEEEVEDRKMKGLMRRVTEILMEPN 695
I EGKVV++R++ AT+FNMLS+IL+SRDLVSLE E ED M +++ + + PN
Sbjct: 168 FINKMEGKVVKIREVATATVFNMLSNILVSRDLVSLEHESEDGGMSSVLKDIARLASTPN 227
Query: 696 ISDIFPALNSLDLQGVHKKGKKLWKEIWDIFEPLVEEKR-----LRKSKENAEKQDFLDT 860
ISD +P L LDLQG+ KK +L + +++ E +++E+R R + + ++DFLD
Sbjct: 228 ISDFYPILGPLDLQGLRKKTMELHRRSFNMCEAIIQERREGGEGKRDGPDASRRRDFLDA 287
Query: 861 LVALQFSDDEVQELLVELFSAGSDTSTSTIEWAMAELVKNPEAMKNLQDELEREISQDL- 1037
L+ SDD++ LL+EL SAG+DTS+STIEW MAEL+KNP +K +Q+E+ I+ +
Sbjct: 288 LILNGSSDDQIDILLMELLSAGTDTSSSTIEWTMAELIKNPRCLKKVQEEIANVINMNRD 347
Query: 1038 --IKETHLMQLPYLQACVKETLRLHPAAPLLIPRKPIEACRVMNYTIPKNSQVLVNVWAI 1211
KE+HL QL YLQACVKETLRLHP P L+P + I++C+VMNYTIPKN+QVLVN WAI
Sbjct: 348 TGFKESHLPQLTYLQACVKETLRLHPPGPFLLPHRAIDSCQVMNYTIPKNTQVLVNYWAI 407
Query: 1212 GRDQTTWEDPMEFKPERFLNSSLDFKGNDFEFLPFGGGRRICPGYPMAMRQIPLAIASMI 1391
GRD +WE+P+ F PERFL+S+LDFKGNDFEF+PFG GRRICPG PMA + + L IA +I
Sbjct: 408 GRDPKSWEEPVVFNPERFLSSNLDFKGNDFEFIPFGSGRRICPGLPMAAKHVALIIAYLI 467
Query: 1392 HYFNWYLPKGTTPDMLDMSESFGLTLEKEHPLLIIPRARR 1511
+F+W LP G P LDMSE++GLTL KE PLL++P +++
Sbjct: 468 LFFDWSLPCGKNPTDLDMSENYGLTLRKEQPLLLVPTSKK 507