BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum22_contig00002441
         (2059 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

dbj|BAA08365.1| 4-coumarate:CoA ligase [Lithospermum erythrorhizon]  1025   0.0  
gb|ABD59789.2| 4-coumarate:CoA ligase [Arnebia euchroma]             1017   0.0  
sp|O24146.1|4CL2_TOBAC RecName: Full=4-coumarate--CoA ligase 2; ...   892   0.0  
dbj|BAA07828.1| 4-coumarate:coenzyme A ligase [Nicotiana tabacum]     881   0.0  
sp|O24145.1|4CL1_TOBAC RecName: Full=4-coumarate--CoA ligase 1; ...   880   0.0  

>dbj|BAA08365.1| 4-coumarate:CoA ligase [Lithospermum erythrorhizon]
          Length = 636

 Score = 1025 bits (2650), Expect = 0.0
 Identities = 517/598 (86%), Positives = 551/598 (92%), Gaps = 2/598 (0%)
 Frame = +1

Query: 76   MDTQTKTDQKDIIFKSKLPDIFIPKHLPLHSYCFQNISDFNTRPCLINGSNNQIYTYSQV 255
            MDTQTKTDQKDIIF+SKLPDI+IPKHLPLHSYC +NIS F++RPCLINGSN+++YTY++V
Sbjct: 1    MDTQTKTDQKDIIFRSKLPDIYIPKHLPLHSYCGENISQFSSRPCLINGSNDRVYTYAEV 60

Query: 256  ELISRKVASGLHKHGIKKNETIMILLPNCPEFVFAFLGASYIGAVSTTANPFFTSSEIIK 435
            E+ SRKVA+GLHKHGIK+ ETIM+LLPNCPEFVFAFLGASYIGAVSTTANPFFTSSEIIK
Sbjct: 61   EITSRKVAAGLHKHGIKQTETIMLLLPNCPEFVFAFLGASYIGAVSTTANPFFTSSEIIK 120

Query: 436  QAKASKTKLIITEATNVPKFKDFSQENDIKIMCIDEQIEGCLHFSSVLENSDETTLPDVE 615
            QAKASKTKLIIT +T VPK KDFSQEN +KIMCID++I+GCLHFSS LENSDETTLPDVE
Sbjct: 121  QAKASKTKLIITVSTTVPKLKDFSQENHVKIMCIDDKIDGCLHFSSDLENSDETTLPDVE 180

Query: 616  IHPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHKEDVVMCTLPLFH 795
            I PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDN NLYMH EDVVMCTLPLFH
Sbjct: 181  IRPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNANLYMHHEDVVMCTLPLFH 240

Query: 796  IYSMNSILLCGLRVGATILLMHKFEIVTFLELIQRFKVTIGPFVPPIVLAIAKSPVVDQF 975
            IYSMNSILLCGLRVGA ILLMHKFEIVTFLELIQR+KVTIGPFVPPIVLAIAKS VVDQ+
Sbjct: 241  IYSMNSILLCGLRVGAAILLMHKFEIVTFLELIQRYKVTIGPFVPPIVLAIAKSNVVDQY 300

Query: 976  DLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFPIKS 1155
            DLS+VRTVMSGAAPLG ELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF IKS
Sbjct: 301  DLSTVRTVMSGAAPLGSELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKS 360

Query: 1156 GACGTVVRNAHMKIVDVETGASLPRNQSGEICIRGDQIMKGYLNDPEATERTIDKEGWLH 1335
            GACGTVVRN+ MKI+D ETGASLPRNQSGEICIRGDQIMKGYLNDPEATERTIDKEGWLH
Sbjct: 361  GACGTVVRNSEMKIIDTETGASLPRNQSGEICIRGDQIMKGYLNDPEATERTIDKEGWLH 420

Query: 1336 TGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLVTHPQISDAAVVPMKDEGAGE 1515
            TGDIGYIDDDDELFIVDRLKELIKYKGFQVAP ELEALLV HP +SDAAVV MKDEGAGE
Sbjct: 421  TGDIGYIDDDDELFIVDRLKELIKYKGFQVAPPELEALLVPHPNVSDAAVVSMKDEGAGE 480

Query: 1516 VPVAFVVTSSGFITTEDEIKKFVSKQVIFYKRINRVFFVDSIPKSPSGKILRKDLRAKLA 1695
            VPVAFVV S+G  TTEDEIK+FVSKQVIFYKRINRVF VDSIPKSPSGKI+RKDLRAKLA
Sbjct: 481  VPVAFVVRSNGSTTTEDEIKQFVSKQVIFYKRINRVFGVDSIPKSPSGKIVRKDLRAKLA 540

Query: 1696 DMYPNDPTTKVVPN--DVTTKDVPNSVSMEGLIIANGGVAGEEIANGAPTNAPDGVAT 1863
              + N PTT VVPN  +V   +VPN VS  G+  ANGGVA E +ANG PT+   GVAT
Sbjct: 541  ARFLNGPTTNVVPNGGNVAKDNVPNGVS-NGVSKANGGVAKEGVANGVPTDGDYGVAT 597


>gb|ABD59789.2| 4-coumarate:CoA ligase [Arnebia euchroma]
          Length = 660

 Score = 1017 bits (2629), Expect = 0.0
 Identities = 520/629 (82%), Positives = 552/629 (87%), Gaps = 33/629 (5%)
 Frame = +1

Query: 76   MDTQTKTDQKDIIFKSKLPDIFIPKHLPLHSYCFQNISDFNTRPCLINGSNNQIYTYSQV 255
            MDTQTKTDQKDIIF+SKLPDI+IP+HLPLHSYCF+NIS FN+RPCLINGSN+++YTY++V
Sbjct: 1    MDTQTKTDQKDIIFRSKLPDIYIPRHLPLHSYCFENISQFNSRPCLINGSNDRVYTYAEV 60

Query: 256  ELISRKVASGLHKHGIKKNETIMILLPNCPEFVFAFLGASYIGAVSTTANPFFTSSEIIK 435
            EL SRKVA+GLHKHGIK+ ETIM+LLPNCPEFVFAFLGASYIGAVSTTANPFFTSSEIIK
Sbjct: 61   ELTSRKVAAGLHKHGIKQTETIMLLLPNCPEFVFAFLGASYIGAVSTTANPFFTSSEIIK 120

Query: 436  QAKASKTKLIITEATNVPKFKDFSQENDIKIMCIDEQIEGCLHFSSVLENSDETTLPDVE 615
            QAKASKTKLIIT A+NVPK K+FSQEN +KIMCIDEQIEGCLHFS  LEN+DETTLP+VE
Sbjct: 121  QAKASKTKLIITVASNVPKLKEFSQENGVKIMCIDEQIEGCLHFSLDLENTDETTLPEVE 180

Query: 616  IHPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHKEDVVMCTLPLFH 795
            I P+DVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDG+N NLYMH+EDVVMCTLPLFH
Sbjct: 181  ILPNDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHQEDVVMCTLPLFH 240

Query: 796  IYSMNSILLCGLRVGATILLMHKFEIVTFLELIQRFKVTIGPFVPPIVLAIAKSPVVDQF 975
            IYSMNSILLCGLRVGA ILLMHKF+I  FLELIQR+KVTIGPFVPPIVLAIAKS VVDQF
Sbjct: 241  IYSMNSILLCGLRVGAAILLMHKFDIAPFLELIQRYKVTIGPFVPPIVLAIAKSNVVDQF 300

Query: 976  DLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFPIKS 1155
            DLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF IKS
Sbjct: 301  DLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKS 360

Query: 1156 GACGTVVRNAHMKIVDVETGASLPRNQSGEICIRGDQIMKGYLNDPEATERTIDKEGWLH 1335
            GACGTVVRNA MKI+D ETG SLPRNQSGEICIRGDQIMKGYLNDPEATERTID EGWLH
Sbjct: 361  GACGTVVRNAEMKIIDTETGVSLPRNQSGEICIRGDQIMKGYLNDPEATERTIDNEGWLH 420

Query: 1336 TGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLVTHPQISDAAVVPMKDEGAGE 1515
            TGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLVTHP++SDAAVV MKDEGAGE
Sbjct: 421  TGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLVTHPKVSDAAVVSMKDEGAGE 480

Query: 1516 VPVAFVVTSSGFITTEDEIKKFVSKQVIFYKRINRVFFVDSIPKSPSGKILRKDLRAKLA 1695
            VPVAFVV S+G   TEDEIK+FVSKQVIFYKRINRVFFVDSIPKSPSGKILRKDLRAKLA
Sbjct: 481  VPVAFVVRSNGSTITEDEIKQFVSKQVIFYKRINRVFFVDSIPKSPSGKILRKDLRAKLA 540

Query: 1696 DMYPNDPTTKV--VPNDVTTKDVPNSVS-------------------------------M 1776
              + N PTT    VPN    KDVPN VS                                
Sbjct: 541  AGFLNGPTTTTNGVPNSGVAKDVPNGVSNGVSKANGGGIEEGIANGVAKANGGVAKEEIA 600

Query: 1777 EGLIIANGGVAGEEIANGAPTNAPDGVAT 1863
             G+  AN GVA EEIANG PT+  +GVAT
Sbjct: 601  NGVSKANCGVAKEEIANGIPTSVANGVAT 629


>sp|O24146.1|4CL2_TOBAC RecName: Full=4-coumarate--CoA ligase 2; Short=4CL 2; AltName:
            Full=4-coumaroyl-CoA synthase 2 gi|1663724|gb|AAB18638.1|
            4-coumarate:coenzyme A ligase [Nicotiana tabacum]
          Length = 542

 Score =  892 bits (2304), Expect = 0.0
 Identities = 440/539 (81%), Positives = 488/539 (90%)
 Frame = +1

Query: 94   TDQKDIIFKSKLPDIFIPKHLPLHSYCFQNISDFNTRPCLINGSNNQIYTYSQVELISRK 273
            T Q DIIF+SKLPDI+IP HLPLHSYCF+NIS+F++RPCLING+N QIYTY+ VEL SRK
Sbjct: 5    TKQVDIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELNSRK 64

Query: 274  VASGLHKHGIKKNETIMILLPNCPEFVFAFLGASYIGAVSTTANPFFTSSEIIKQAKASK 453
            VA+GLHK GI+  +TIMILLPN PEFVFAF+GASY+GA+ST ANP FT +E++KQAKAS 
Sbjct: 65   VAAGLHKQGIQPKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASS 124

Query: 454  TKLIITEATNVPKFKDFSQENDIKIMCIDEQIEGCLHFSSVLENSDETTLPDVEIHPDDV 633
             K+I+T+A +V K KD++ END+KI+CID   EGCLHFS VL  ++E  +P+VEI PDDV
Sbjct: 125  AKIIVTQACHVNKVKDYAFENDVKIICIDSAPEGCLHFS-VLTQANEHDIPEVEIQPDDV 183

Query: 634  VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHKEDVVMCTLPLFHIYSMNS 813
            VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDG+NPNLY+H EDV++C LPLFHIYS+NS
Sbjct: 184  VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYIHSEDVMLCVLPLFHIYSLNS 243

Query: 814  ILLCGLRVGATILLMHKFEIVTFLELIQRFKVTIGPFVPPIVLAIAKSPVVDQFDLSSVR 993
            +LLCGLRVGA IL+M KF+IV+FLELIQR+KVTIGPFVPPIVLAIAKSP+VD +DLSSVR
Sbjct: 244  VLLCGLRVGAAILIMQKFDIVSFLELIQRYKVTIGPFVPPIVLAIAKSPMVDDYDLSSVR 303

Query: 994  TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFPIKSGACGTV 1173
            TVMSGAAPLGKELED VRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF IKSGACGTV
Sbjct: 304  TVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTV 363

Query: 1174 VRNAHMKIVDVETGASLPRNQSGEICIRGDQIMKGYLNDPEATERTIDKEGWLHTGDIGY 1353
            VRNA MKIVD +TG SLPRNQSGEICIRGDQIMKGYLNDPEAT RTIDKEGWL+TGDIGY
Sbjct: 364  VRNAEMKIVDPKTGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGY 423

Query: 1354 IDDDDELFIVDRLKELIKYKGFQVAPAELEALLVTHPQISDAAVVPMKDEGAGEVPVAFV 1533
            IDDDDELFIVDRLKELIKYKGFQVAPAELEALL+ HP ISDAAVVPMKDE AGEVPVAFV
Sbjct: 424  IDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPNISDAAVVPMKDEQAGEVPVAFV 483

Query: 1534 VTSSGFITTEDEIKKFVSKQVIFYKRINRVFFVDSIPKSPSGKILRKDLRAKLADMYPN 1710
            V S+G   TEDE+K F+SKQVIFYKRI RVFFVD+IPKSPSGKILRKDLRAKLA   PN
Sbjct: 484  VRSNGSTITEDEVKDFISKQVIFYKRIKRVFFVDAIPKSPSGKILRKDLRAKLAAGLPN 542


>dbj|BAA07828.1| 4-coumarate:coenzyme A ligase [Nicotiana tabacum]
          Length = 542

 Score =  881 bits (2276), Expect = 0.0
 Identities = 435/539 (80%), Positives = 483/539 (89%)
 Frame = +1

Query: 94   TDQKDIIFKSKLPDIFIPKHLPLHSYCFQNISDFNTRPCLINGSNNQIYTYSQVELISRK 273
            T   DIIF+SKLPDI+IP HLPLHSYCF+NIS+F++RPCLING+N QIYTY+ VEL SRK
Sbjct: 5    TKHGDIIFRSKLPDIYIPNHLPLHSYCFENISEFSSRPCLINGANKQIYTYADVELSSRK 64

Query: 274  VASGLHKHGIKKNETIMILLPNCPEFVFAFLGASYIGAVSTTANPFFTSSEIIKQAKASK 453
            VA+GLHK GI++ +TIMILLPN PEFVFAF+GASY+GA+ST ANP FT++E++KQ KAS 
Sbjct: 65   VAAGLHKQGIQQKDTIMILLPNSPEFVFAFIGASYLGAISTMANPLFTAAEVVKQVKASG 124

Query: 454  TKLIITEATNVPKFKDFSQENDIKIMCIDEQIEGCLHFSSVLENSDETTLPDVEIHPDDV 633
             K+I+T+A +V K KD++ EN++KI+CID   EGCLHFS VL  +DE  +P+VEI PDDV
Sbjct: 125  AKIIVTQACHVNKVKDYALENNVKIICIDSAPEGCLHFS-VLTQADEHDIPEVEIQPDDV 183

Query: 634  VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHKEDVVMCTLPLFHIYSMNS 813
            VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDG+N NLY+H EDV++C LPLFHIYS+NS
Sbjct: 184  VALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENRNLYIHSEDVLLCVLPLFHIYSLNS 243

Query: 814  ILLCGLRVGATILLMHKFEIVTFLELIQRFKVTIGPFVPPIVLAIAKSPVVDQFDLSSVR 993
            +LLCGLRVGA IL+M KF+IV FLELIQ +KVTIGPFVPPIVLAIAKSP+VD +DLSSVR
Sbjct: 244  VLLCGLRVGAAILIMQKFDIVPFLELIQNYKVTIGPFVPPIVLAIAKSPMVDDYDLSSVR 303

Query: 994  TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFPIKSGACGTV 1173
            TVMSGAAPLGKELED VRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF IKSGACGTV
Sbjct: 304  TVMSGAAPLGKELEDTVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFEIKSGACGTV 363

Query: 1174 VRNAHMKIVDVETGASLPRNQSGEICIRGDQIMKGYLNDPEATERTIDKEGWLHTGDIGY 1353
            VRNA MKIVD ETG SLPRNQSGEICIRGDQIMKGYLNDPEAT RTIDKEGWL+TGDIGY
Sbjct: 364  VRNAEMKIVDPETGNSLPRNQSGEICIRGDQIMKGYLNDPEATARTIDKEGWLYTGDIGY 423

Query: 1354 IDDDDELFIVDRLKELIKYKGFQVAPAELEALLVTHPQISDAAVVPMKDEGAGEVPVAFV 1533
            IDDDDELFIVDRLKELIKYKGFQVAPAELEALL+ HP  SDAAVVPMKDE A EVPVAFV
Sbjct: 424  IDDDDELFIVDRLKELIKYKGFQVAPAELEALLLNHPTFSDAAVVPMKDEQAEEVPVAFV 483

Query: 1534 VTSSGFITTEDEIKKFVSKQVIFYKRINRVFFVDSIPKSPSGKILRKDLRAKLADMYPN 1710
            V SSG   TEDE+K F+SKQVIFYKRI RVFFVD++PKSPSGKILRKDLRAKLA   PN
Sbjct: 484  VRSSGSTITEDEVKDFISKQVIFYKRIKRVFFVDAVPKSPSGKILRKDLRAKLAAGLPN 542


>sp|O24145.1|4CL1_TOBAC RecName: Full=4-coumarate--CoA ligase 1; Short=4CL 1; AltName:
            Full=4-coumaroyl-CoA synthase 1 gi|1663722|gb|AAB18637.1|
            4-coumarate:coenzyme A ligase [Nicotiana tabacum]
          Length = 547

 Score =  880 bits (2273), Expect = 0.0
 Identities = 432/546 (79%), Positives = 489/546 (89%), Gaps = 1/546 (0%)
 Frame = +1

Query: 76   MDTQTKTDQK-DIIFKSKLPDIFIPKHLPLHSYCFQNISDFNTRPCLINGSNNQIYTYSQ 252
            M+T T+T Q  D+IF+SKLPDI+IPKHLPLHSYCF+NIS+F++RPCLING+N+QIYTY++
Sbjct: 3    METTTETKQSGDLIFRSKLPDIYIPKHLPLHSYCFENISEFSSRPCLINGANDQIYTYAE 62

Query: 253  VELISRKVASGLHKHGIKKNETIMILLPNCPEFVFAFLGASYIGAVSTTANPFFTSSEII 432
            VEL  RKVA GL+K GI++ +TIMILLPN PEFVFAF+GASY+GA+ST ANP FT +E++
Sbjct: 63   VELTCRKVAVGLNKLGIQQKDTIMILLPNSPEFVFAFMGASYLGAISTMANPLFTPAEVV 122

Query: 433  KQAKASKTKLIITEATNVPKFKDFSQENDIKIMCIDEQIEGCLHFSSVLENSDETTLPDV 612
            KQAKAS  K+IIT++  V K KD++ END+K++CID   EGCLHFS  L  SDE  +P+V
Sbjct: 123  KQAKASSAKIIITQSCFVGKVKDYASENDVKVICIDSAPEGCLHFSE-LTQSDEHEIPEV 181

Query: 613  EIHPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHKEDVVMCTLPLF 792
            +I PDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDG+N NLYMH EDV+MC LPLF
Sbjct: 182  KIQPDDVVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSEDVLMCVLPLF 241

Query: 793  HIYSMNSILLCGLRVGATILLMHKFEIVTFLELIQRFKVTIGPFVPPIVLAIAKSPVVDQ 972
            HIYS+NSILLCGLRVGA IL+M KF+I  FLELIQ++KV+IGPFVPPIVLAIAKSP+VD 
Sbjct: 242  HIYSLNSILLCGLRVGAAILIMQKFDIAPFLELIQKYKVSIGPFVPPIVLAIAKSPIVDS 301

Query: 973  FDLSSVRTVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFPIK 1152
            +DLSSVRTVMSGAAPLGKELEDAVR KFPNAKLGQGYGMTEAGPVLAMCLAFAKEPF IK
Sbjct: 302  YDLSSVRTVMSGAAPLGKELEDAVRTKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIK 361

Query: 1153 SGACGTVVRNAHMKIVDVETGASLPRNQSGEICIRGDQIMKGYLNDPEATERTIDKEGWL 1332
            SGACGTVVRNA MKIVD +TG SLPRNQ GEICIRGDQIMKGYLNDPEAT RTIDKEGWL
Sbjct: 362  SGACGTVVRNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATTRTIDKEGWL 421

Query: 1333 HTGDIGYIDDDDELFIVDRLKELIKYKGFQVAPAELEALLVTHPQISDAAVVPMKDEGAG 1512
            HTGDIG+ID+DDELFIVDRLKELIKYKGFQVAPAE+EALL+ HP ISDAAVVPMKDE AG
Sbjct: 422  HTGDIGFIDEDDELFIVDRLKELIKYKGFQVAPAEIEALLLNHPNISDAAVVPMKDEQAG 481

Query: 1513 EVPVAFVVTSSGFITTEDEIKKFVSKQVIFYKRINRVFFVDSIPKSPSGKILRKDLRAKL 1692
            EVPVAFVV S+G   TEDE+K F+SKQVIFYKR+ RVFFV+++PKSPSGKILRKDLRA+L
Sbjct: 482  EVPVAFVVRSNGSAITEDEVKDFISKQVIFYKRVKRVFFVETVPKSPSGKILRKDLRARL 541

Query: 1693 ADMYPN 1710
            A   PN
Sbjct: 542  AAGVPN 547