BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum22_contig00001412
         (689 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002522423.1| sur2 hydroxylase/desaturase, putative [Ricin...    96   4e-34
ref|XP_002275229.1| PREDICTED: sphingoid base hydroxylase 2 [Vit...    96   3e-33
gb|ABO92998.1| putative sterol desaturase [Solanum tuberosum]          95   6e-33
gb|ABO93014.1| putative sterol desaturase [Solanum tuberosum]          94   8e-33
ref|NP_177122.2| sphingoid base hydroxylase 1 [Arabidopsis thali...    96   5e-32

>ref|XP_002522423.1| sur2 hydroxylase/desaturase, putative [Ricinus communis]
           gi|223538308|gb|EEF39915.1| sur2 hydroxylase/desaturase,
           putative [Ricinus communis]
          Length = 258

 Score = 96.3 bits (238), Expect(2) = 4e-34
 Identities = 43/47 (91%), Positives = 47/47 (100%)
 Frame = +2

Query: 548 NKFLYRNIHSQHHRLIVPYAFGALYNHPVEGLILDTIGGALSFLLSG 688
           NKFLY++IHSQHHRL+VPYAFGALYNHPVEGL+LDTIGGALSFLLSG
Sbjct: 118 NKFLYKHIHSQHHRLVVPYAFGALYNHPVEGLLLDTIGGALSFLLSG 164



 Score = 74.7 bits (182), Expect(2) = 4e-34
 Identities = 44/127 (34%), Positives = 58/127 (45%)
 Frame = +1

Query: 67  FNVTDELLGTFVPILIYWVYSGVYVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 246
           F ++DELLGTF+PI+IYWVYSG+Y+                                   
Sbjct: 3   FQISDELLGTFLPIVIYWVYSGLYIGLGYFDNY--------------------------- 35

Query: 247 XRLHSRKEEDEKNLVPKKEXXXXXXXXXXXXXXXXXXXXXXTGSDVETKDDQLPSIFVIA 426
            RLH++++EDEKNLV K                        TG D ET  DQ PS+ ++A
Sbjct: 36  -RLHTKQDEDEKNLVSKSTVVKGVLLQQAIQAVVAIILFTVTGDDSETAMDQKPSLMILA 94

Query: 427 RQFLIAM 447
           RQF+ AM
Sbjct: 95  RQFVTAM 101


>ref|XP_002275229.1| PREDICTED: sphingoid base hydroxylase 2 [Vitis vinifera]
           gi|296086379|emb|CBI31968.3| unnamed protein product
           [Vitis vinifera]
          Length = 258

 Score = 96.3 bits (238), Expect(2) = 3e-33
 Identities = 43/47 (91%), Positives = 47/47 (100%)
 Frame = +2

Query: 548 NKFLYRNIHSQHHRLIVPYAFGALYNHPVEGLILDTIGGALSFLLSG 688
           NKFLYR+IHSQHHRL+VPYAFGALYNHP+EGL+LDTIGGALSFLLSG
Sbjct: 118 NKFLYRHIHSQHHRLVVPYAFGALYNHPLEGLLLDTIGGALSFLLSG 164



 Score = 72.0 bits (175), Expect(2) = 3e-33
 Identities = 45/129 (34%), Positives = 55/129 (42%)
 Frame = +1

Query: 61  LGFNVTDELLGTFVPILIYWVYSGVYVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 240
           +GF V+DELLG FVPIL+YWVYSG+Y+                                 
Sbjct: 1   MGFEVSDELLGAFVPILVYWVYSGIYI----------------------------PLGSL 32

Query: 241 XXXRLHSRKEEDEKNLVPKKEXXXXXXXXXXXXXXXXXXXXXXTGSDVETKDDQLPSIFV 420
              RLHS+KEE++KNLV K                        TG D        PS+ V
Sbjct: 33  ENYRLHSKKEEEDKNLVSKGTVVKGVILQQVIQAVVAILLFTVTGDDSGAAAGPKPSLIV 92

Query: 421 IARQFLIAM 447
           +ARQF IAM
Sbjct: 93  LARQFFIAM 101


>gb|ABO92998.1| putative sterol desaturase [Solanum tuberosum]
          Length = 269

 Score = 94.7 bits (234), Expect(2) = 6e-33
 Identities = 42/48 (87%), Positives = 48/48 (100%)
 Frame = +2

Query: 545 QNKFLYRNIHSQHHRLIVPYAFGALYNHPVEGLILDTIGGALSFLLSG 688
           QNKFLY++IH+QHHRLIVPYAFGALYNHP+EGLILDTIGGAL+FL+SG
Sbjct: 128 QNKFLYKHIHAQHHRLIVPYAFGALYNHPLEGLILDTIGGALAFLVSG 175



 Score = 72.4 bits (176), Expect(2) = 6e-33
 Identities = 46/128 (35%), Positives = 58/128 (45%)
 Frame = +1

Query: 64  GFNVTDELLGTFVPILIYWVYSGVYVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 243
           G  V+DE+LGTF+PI++YWVYSG+Y+                                  
Sbjct: 13  GGYVSDEVLGTFLPIVVYWVYSGLYLMLGNMDNY-------------------------- 46

Query: 244 XXRLHSRKEEDEKNLVPKKEXXXXXXXXXXXXXXXXXXXXXXTGSDVETKDDQLPSIFVI 423
             RLHS+K+EDEKNLV KKE                      TG D E+  D+  SIFV+
Sbjct: 47  --RLHSKKDEDEKNLVSKKEVVKGVLLQQIVQAAVATVLFAVTGDDGESGGDRHTSIFVL 104

Query: 424 ARQFLIAM 447
            RQ  IAM
Sbjct: 105 GRQLFIAM 112


>gb|ABO93014.1| putative sterol desaturase [Solanum tuberosum]
          Length = 269

 Score = 94.4 bits (233), Expect(2) = 8e-33
 Identities = 41/48 (85%), Positives = 48/48 (100%)
 Frame = +2

Query: 545 QNKFLYRNIHSQHHRLIVPYAFGALYNHPVEGLILDTIGGALSFLLSG 688
           QNKFLY++IH+QHHRLI+PYAFGALYNHP+EGLILDTIGGAL+FL+SG
Sbjct: 128 QNKFLYKHIHAQHHRLIIPYAFGALYNHPLEGLILDTIGGALAFLVSG 175



 Score = 72.4 bits (176), Expect(2) = 8e-33
 Identities = 45/128 (35%), Positives = 58/128 (45%)
 Frame = +1

Query: 64  GFNVTDELLGTFVPILIYWVYSGVYVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 243
           G  V+DE+LGTF+PI++YWVYSG+Y+                                  
Sbjct: 13  GGYVSDEILGTFLPIVVYWVYSGLYLMLGTMDNY-------------------------- 46

Query: 244 XXRLHSRKEEDEKNLVPKKEXXXXXXXXXXXXXXXXXXXXXXTGSDVETKDDQLPSIFVI 423
             RLHS+K+EDEKNLV KKE                      TG D E+  D+  SIFV+
Sbjct: 47  --RLHSKKDEDEKNLVSKKEVVKGVLLQQIVQAAVATVLFAVTGDDGESGGDRHASIFVL 104

Query: 424 ARQFLIAM 447
            RQ  +AM
Sbjct: 105 GRQLFVAM 112


>ref|NP_177122.2| sphingoid base hydroxylase 1 [Arabidopsis thaliana]
           gi|75248486|sp|Q8VYI1.1|SBH1_ARATH RecName:
           Full=Sphinganine C(4)-monooxygenase 1; AltName:
           Full=Sphingoid C4-hydroxylase 1; AltName: Full=Sphingoid
           base hydroxylase 1 gi|17979535|gb|AAL50102.1|
           At1g69640/F24J1.22 [Arabidopsis thaliana]
           gi|23505995|gb|AAN28857.1| At1g69640/F24J1.22
           [Arabidopsis thaliana] gi|332196837|gb|AEE34958.1|
           sphingoid base hydroxylase 1 [Arabidopsis thaliana]
          Length = 260

 Score = 96.3 bits (238), Expect(2) = 5e-32
 Identities = 43/48 (89%), Positives = 48/48 (100%)
 Frame = +2

Query: 545 QNKFLYRNIHSQHHRLIVPYAFGALYNHPVEGLILDTIGGALSFLLSG 688
           QNKFLY++IHSQHHRLIVPYA+GALYNHPVEGL+LDTIGGALSFL+SG
Sbjct: 119 QNKFLYKHIHSQHHRLIVPYAYGALYNHPVEGLLLDTIGGALSFLVSG 166



 Score = 67.8 bits (164), Expect(2) = 5e-32
 Identities = 44/130 (33%), Positives = 55/130 (42%)
 Frame = +1

Query: 58  ILGFNVTDELLGTFVPILIYWVYSGVYVXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 237
           ++GF V+DELLGT  PI++YW+YSG+YV                                
Sbjct: 2   MMGFAVSDELLGTVAPIVVYWLYSGIYV----------------------------ALSS 33

Query: 238 XXXXRLHSRKEEDEKNLVPKKEXXXXXXXXXXXXXXXXXXXXXXTGSDVETKDDQLPSIF 417
               RLHS+ EE+EKNLV K                        TGSD E    Q  S  
Sbjct: 34  LESYRLHSKVEEEEKNLVSKSSVVKGVLVQQVVQAVVAILLFTVTGSDAEADKAQQFSFL 93

Query: 418 VIARQFLIAM 447
           V+ARQF+ AM
Sbjct: 94  VLARQFVTAM 103