BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Lithospermum22_contig00001244
         (1871 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CAL69911.1| obtusifoliol-14-demethylase [Petunia x hybrida]       828   0.0  
gb|AAL54888.1|AF116915_1 obtusifoliol-14-demethylase [Nicotiana ...   821   0.0  
ref|NP_001234537.1| obtusifoliol 14alpha-demethylase [Solanum ly...   819   0.0  
gb|AAL40888.1| obtusifoliol-14-demethylase [Nicotiana tabacum]        818   0.0  
gb|ACE06982.1| obtusifoliol-14alpha-demethylase [Gossypium hirsu...   816   0.0  

>emb|CAL69911.1| obtusifoliol-14-demethylase [Petunia x hybrida]
          Length = 492

 Score =  828 bits (2139), Expect = 0.0
 Identities = 402/483 (83%), Positives = 436/483 (90%)
 Frame = +3

Query: 150  DKLMNMGXXXXXXXXXXXXISVLIMPRSSKRLPPSLKAWPPLVGGLIQFLKGPIVMLRKE 329
            +K+MN+G            IS LIMPRS KRLPPS+KAWP L GGL +FLKGP+VMLR+E
Sbjct: 7    NKIMNVGLLLVATLVVAKLISALIMPRSKKRLPPSVKAWPVL-GGLFRFLKGPVVMLREE 65

Query: 330  YGKLGSVFTVNLLHKKITFLIGPEVSAHFFKAPESDLSQQEVYQFNVPTFGPGVVFDVDY 509
            Y KLGSVFT+NLL+KKITF IGPEVSAHFFKAPE+DLSQQEVYQFNVPTFGPGVVFDVDY
Sbjct: 66   YPKLGSVFTLNLLNKKITFFIGPEVSAHFFKAPETDLSQQEVYQFNVPTFGPGVVFDVDY 125

Query: 510  SIRQEQFRFFTEALRVNKLKGYVDQMVFEAEDYFSNWGETGEVDLKYELEHLIILTASRC 689
            +IRQEQFRFFTE+LRV KLKGYVDQMV EAE+YFS WG++GEVDLKYELEHLIILTASRC
Sbjct: 126  TIRQEQFRFFTESLRVTKLKGYVDQMVMEAEEYFSKWGDSGEVDLKYELEHLIILTASRC 185

Query: 690  LLGQEVRNQLFDDVSALFHDLDNGMLPISVLFPYLPIPAHRRRDQARKKIAQIFGNIIAS 869
            LLG+EVRN+LF+DVSALFHDLDNGMLPISV+FPYL IPAHRRRD ARKK+AQIF NIIAS
Sbjct: 186  LLGEEVRNKLFEDVSALFHDLDNGMLPISVIFPYLSIPAHRRRDNARKKLAQIFANIIAS 245

Query: 870  RKQAGEAEHDMLQCFIDSKYKDGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAYLLCN 1049
            RK+ G+AE DMLQCFIDSKYKDGRPTTESE+TGLLIAALFAGQHTSSITSTWTGAYLLCN
Sbjct: 246  RKRTGKAESDMLQCFIDSKYKDGRPTTESEITGLLIAALFAGQHTSSITSTWTGAYLLCN 305

Query: 1050 QKYMSAVQEEQKELMKKHGTKIDHDILSEMEVLYRCIKEALRLHPPLIMLLRQSHSDFSV 1229
             KYMSAV +EQK LMKKHG K+DHDILSEMEVLYRCIKEALRLHPPLIMLLR SHSDF+V
Sbjct: 306  NKYMSAVVDEQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLRSSHSDFTV 365

Query: 1230 KTKDGKEYDIPKGHIVATSPAFANRLPHIYKDPDTYDPDRFSPGRDEDKAAGAFSYVSFG 1409
            KT++GKEYDIPKGHIVATSPAFANRLPHIYK+PDTYDPDRF+PGR+EDK AGAFSY+SFG
Sbjct: 366  KTREGKEYDIPKGHIVATSPAFANRLPHIYKNPDTYDPDRFAPGREEDKTAGAFSYISFG 425

Query: 1410 GGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNAXXXXXXXXXXXRYKRRAL 1589
            GGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNA           +YKRR L
Sbjct: 426  GGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNAMVVGVKGEVMVKYKRRKL 485

Query: 1590 PVE 1598
              +
Sbjct: 486  STD 488


>gb|AAL54888.1|AF116915_1 obtusifoliol-14-demethylase [Nicotiana tabacum]
          Length = 487

 Score =  821 bits (2121), Expect = 0.0
 Identities = 395/483 (81%), Positives = 434/483 (89%)
 Frame = +3

Query: 150  DKLMNMGXXXXXXXXXXXXISVLIMPRSSKRLPPSLKAWPPLVGGLIQFLKGPIVMLRKE 329
            +K++N G            IS LIMPRS KRLPP +K+WP ++GGL++FLKGP+VMLR+E
Sbjct: 6    NKILNAGLLLVATLVVAKLISALIMPRSKKRLPPVIKSWP-ILGGLLRFLKGPVVMLREE 64

Query: 330  YGKLGSVFTVNLLHKKITFLIGPEVSAHFFKAPESDLSQQEVYQFNVPTFGPGVVFDVDY 509
            Y KLGSVFT+NLL+K ITF +GPEVSAHFFKAPE+DLSQQEVYQFNVPTFGPGVVFDVDY
Sbjct: 65   YPKLGSVFTLNLLNKNITFFVGPEVSAHFFKAPETDLSQQEVYQFNVPTFGPGVVFDVDY 124

Query: 510  SIRQEQFRFFTEALRVNKLKGYVDQMVFEAEDYFSNWGETGEVDLKYELEHLIILTASRC 689
            +IRQEQFRFFTE+LRVNKLKGYVDQMV EAE+YFS WG++GEVDLKYELEHLIILTASRC
Sbjct: 125  TIRQEQFRFFTESLRVNKLKGYVDQMVMEAEEYFSKWGDSGEVDLKYELEHLIILTASRC 184

Query: 690  LLGQEVRNQLFDDVSALFHDLDNGMLPISVLFPYLPIPAHRRRDQARKKIAQIFGNIIAS 869
            LLG+EVRN+LF+DVSALFHDLDNGMLPISV+FPYLPIPAHRRRD ARKK+A+IF NII S
Sbjct: 185  LLGEEVRNKLFEDVSALFHDLDNGMLPISVIFPYLPIPAHRRRDNARKKLAEIFANIIDS 244

Query: 870  RKQAGEAEHDMLQCFIDSKYKDGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAYLLCN 1049
            RK+ G+AE DMLQCFIDSKYKDGR TTESE+TGLLIAALFAGQHTSSITSTW GAYLLCN
Sbjct: 245  RKRTGKAESDMLQCFIDSKYKDGRATTESEITGLLIAALFAGQHTSSITSTWAGAYLLCN 304

Query: 1050 QKYMSAVQEEQKELMKKHGTKIDHDILSEMEVLYRCIKEALRLHPPLIMLLRQSHSDFSV 1229
             KYMSAV +EQK LMKKHG K+DHDILSEMEVLYRCIKEALRLHPPLIMLLR SHSDF+V
Sbjct: 305  NKYMSAVVDEQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLRSSHSDFTV 364

Query: 1230 KTKDGKEYDIPKGHIVATSPAFANRLPHIYKDPDTYDPDRFSPGRDEDKAAGAFSYVSFG 1409
            KT++GKEYDIPKGHIVATSPAFANRLPH+YK+PDTYDPDRF+PGRDEDK AGAFSY+SFG
Sbjct: 365  KTREGKEYDIPKGHIVATSPAFANRLPHVYKNPDTYDPDRFAPGRDEDKVAGAFSYISFG 424

Query: 1410 GGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNAXXXXXXXXXXXRYKRRAL 1589
            GGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNA           +YKRR L
Sbjct: 425  GGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNAMVVGVKGKVMVKYKRRKL 484

Query: 1590 PVE 1598
              E
Sbjct: 485  STE 487


>ref|NP_001234537.1| obtusifoliol 14alpha-demethylase [Solanum lycopersicum]
            gi|299735181|gb|ADJ37071.1| obtusifoliol
            14alpha-demethylase [Solanum lycopersicum]
          Length = 487

 Score =  819 bits (2116), Expect = 0.0
 Identities = 399/483 (82%), Positives = 434/483 (89%)
 Frame = +3

Query: 150  DKLMNMGXXXXXXXXXXXXISVLIMPRSSKRLPPSLKAWPPLVGGLIQFLKGPIVMLRKE 329
            +K++N+G            IS LIMPRS KRLPP +KAWP +VGGLI+FLKGP+VMLR+E
Sbjct: 6    NKILNVGLLLVATLLVAKLISALIMPRSKKRLPPVVKAWP-IVGGLIRFLKGPVVMLRQE 64

Query: 330  YGKLGSVFTVNLLHKKITFLIGPEVSAHFFKAPESDLSQQEVYQFNVPTFGPGVVFDVDY 509
            Y KLGSVFT+NLL+K ITF IGPEVSAHFFKAPE+DLSQQEVYQFNVPTFGPGVVFDVDY
Sbjct: 65   YPKLGSVFTLNLLNKNITFFIGPEVSAHFFKAPETDLSQQEVYQFNVPTFGPGVVFDVDY 124

Query: 510  SIRQEQFRFFTEALRVNKLKGYVDQMVFEAEDYFSNWGETGEVDLKYELEHLIILTASRC 689
            +IRQEQFRFFTEALRV KLKGYVDQMV EAE+YFS WGE+GEVDLKYELEHLIILTASRC
Sbjct: 125  TIRQEQFRFFTEALRVTKLKGYVDQMVTEAEEYFSKWGESGEVDLKYELEHLIILTASRC 184

Query: 690  LLGQEVRNQLFDDVSALFHDLDNGMLPISVLFPYLPIPAHRRRDQARKKIAQIFGNIIAS 869
            LLG+EVRN+LFDDVSALFHDLDNGMLPISV+FPYLPIPAHRRRD ARKK+A+IF NII S
Sbjct: 185  LLGEEVRNKLFDDVSALFHDLDNGMLPISVIFPYLPIPAHRRRDNARKKLAEIFANIINS 244

Query: 870  RKQAGEAEHDMLQCFIDSKYKDGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAYLLCN 1049
            RK+ G+AE+DMLQCFIDSKYKDGRPTTE E+TGLLIAALFAGQHTSSITSTWTG+YLL N
Sbjct: 245  RKRTGKAENDMLQCFIDSKYKDGRPTTEGEITGLLIAALFAGQHTSSITSTWTGSYLLTN 304

Query: 1050 QKYMSAVQEEQKELMKKHGTKIDHDILSEMEVLYRCIKEALRLHPPLIMLLRQSHSDFSV 1229
             KYMSAV +EQK LMKKHG K+DHDILSEMEVLYRCIKEALRLHPPLIMLLR SHS+FSV
Sbjct: 305  DKYMSAVVDEQKNLMKKHGNKVDHDILSEMEVLYRCIKEALRLHPPLIMLLRSSHSEFSV 364

Query: 1230 KTKDGKEYDIPKGHIVATSPAFANRLPHIYKDPDTYDPDRFSPGRDEDKAAGAFSYVSFG 1409
             T++GKEYDI KGHIVATSPAFANRLPHI+K+PDTYDPDRF PGR+EDKAAGAFSY+SFG
Sbjct: 365  TTREGKEYDISKGHIVATSPAFANRLPHIFKNPDTYDPDRFGPGREEDKAAGAFSYISFG 424

Query: 1410 GGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNAXXXXXXXXXXXRYKRRAL 1589
            GGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNA           +YKRR L
Sbjct: 425  GGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNAMVVGVKGEVMVKYKRRKL 484

Query: 1590 PVE 1598
             VE
Sbjct: 485  SVE 487


>gb|AAL40888.1| obtusifoliol-14-demethylase [Nicotiana tabacum]
          Length = 487

 Score =  818 bits (2113), Expect = 0.0
 Identities = 394/482 (81%), Positives = 433/482 (89%)
 Frame = +3

Query: 153  KLMNMGXXXXXXXXXXXXISVLIMPRSSKRLPPSLKAWPPLVGGLIQFLKGPIVMLRKEY 332
            K++N+G            IS LIMPRS KRLPP +K+WP ++GGL++FLKGP+VMLR+EY
Sbjct: 7    KILNVGLLLVVTLVVAKLISALIMPRSKKRLPPVIKSWP-ILGGLLRFLKGPVVMLREEY 65

Query: 333  GKLGSVFTVNLLHKKITFLIGPEVSAHFFKAPESDLSQQEVYQFNVPTFGPGVVFDVDYS 512
             KLGSVFT+NLL+K ITF IGPEVSAHFFKAPE+DLSQQEVYQFNVPTFGPGVVFDVDY+
Sbjct: 66   PKLGSVFTLNLLNKNITFFIGPEVSAHFFKAPETDLSQQEVYQFNVPTFGPGVVFDVDYT 125

Query: 513  IRQEQFRFFTEALRVNKLKGYVDQMVFEAEDYFSNWGETGEVDLKYELEHLIILTASRCL 692
            IRQEQFRFFTEALRVNKLKGYVD MV EAE+YFS WG++GE+DLKYELEHLIILTASRCL
Sbjct: 126  IRQEQFRFFTEALRVNKLKGYVDHMVMEAEEYFSKWGDSGEMDLKYELEHLIILTASRCL 185

Query: 693  LGQEVRNQLFDDVSALFHDLDNGMLPISVLFPYLPIPAHRRRDQARKKIAQIFGNIIASR 872
            LG+EVRN+LF+DVSALFHDLDNGMLPISV+FPYLPIPAHRRRD ARKK+A+IF NII SR
Sbjct: 186  LGEEVRNKLFEDVSALFHDLDNGMLPISVIFPYLPIPAHRRRDNARKKLAEIFANIIDSR 245

Query: 873  KQAGEAEHDMLQCFIDSKYKDGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAYLLCNQ 1052
            K+ G+AE DMLQCFIDSKYKDGR TT+SE+TGLLIAALFAGQHTSSITSTWTGAYLLCN 
Sbjct: 246  KRTGKAESDMLQCFIDSKYKDGRATTDSEITGLLIAALFAGQHTSSITSTWTGAYLLCNN 305

Query: 1053 KYMSAVQEEQKELMKKHGTKIDHDILSEMEVLYRCIKEALRLHPPLIMLLRQSHSDFSVK 1232
            KYMSAV +EQK LMKKHG K+DHDILSEMEVLYRCIKE LRLHPPLIMLLR SHSDF+VK
Sbjct: 306  KYMSAVVDEQKNLMKKHGNKVDHDILSEMEVLYRCIKEVLRLHPPLIMLLRSSHSDFTVK 365

Query: 1233 TKDGKEYDIPKGHIVATSPAFANRLPHIYKDPDTYDPDRFSPGRDEDKAAGAFSYVSFGG 1412
            T++GKEYDIPKGHIVATSPAFANRLPH+YK+PDTYDPDRF+PGRDEDK AGAFSY+SFGG
Sbjct: 366  TREGKEYDIPKGHIVATSPAFANRLPHVYKNPDTYDPDRFTPGRDEDKVAGAFSYISFGG 425

Query: 1413 GRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNAXXXXXXXXXXXRYKRRALP 1592
            GRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNA           +YKRR L 
Sbjct: 426  GRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNAMVVGVKGKVMVKYKRRKLS 485

Query: 1593 VE 1598
             E
Sbjct: 486  NE 487


>gb|ACE06982.1| obtusifoliol-14alpha-demethylase [Gossypium hirsutum]
          Length = 486

 Score =  816 bits (2109), Expect = 0.0
 Identities = 397/486 (81%), Positives = 440/486 (90%)
 Frame = +3

Query: 138  MDNSDKLMNMGXXXXXXXXXXXXISVLIMPRSSKRLPPSLKAWPPLVGGLIQFLKGPIVM 317
            M+ +DKL+N G            IS LIMPRS KR+PP++K WP ++GGL++F+KGPIVM
Sbjct: 1    MEVNDKLLNTGILIVATLVVAKLISFLIMPRSKKRVPPAVKTWP-VIGGLLRFMKGPIVM 59

Query: 318  LRKEYGKLGSVFTVNLLHKKITFLIGPEVSAHFFKAPESDLSQQEVYQFNVPTFGPGVVF 497
            LR+EY KLGSVFT+NL +KKITFLIGPEVS+HFFKA ESDLSQQEVYQFNVPTFGPGVVF
Sbjct: 60   LREEYPKLGSVFTLNLFNKKITFLIGPEVSSHFFKASESDLSQQEVYQFNVPTFGPGVVF 119

Query: 498  DVDYSIRQEQFRFFTEALRVNKLKGYVDQMVFEAEDYFSNWGETGEVDLKYELEHLIILT 677
            DVDY+IRQEQFRFFTEALRVNKLKGYVDQMV EAEDYFS WG++GEVDLKYELEHLIILT
Sbjct: 120  DVDYTIRQEQFRFFTEALRVNKLKGYVDQMVTEAEDYFSKWGDSGEVDLKYELEHLIILT 179

Query: 678  ASRCLLGQEVRNQLFDDVSALFHDLDNGMLPISVLFPYLPIPAHRRRDQARKKIAQIFGN 857
            ASRCLLGQEVRN+LFDDVSALFHDLDNGMLPISV+FPYLPIPAHRRRD+ARKK+A IF N
Sbjct: 180  ASRCLLGQEVRNKLFDDVSALFHDLDNGMLPISVIFPYLPIPAHRRRDRARKKLAGIFAN 239

Query: 858  IIASRKQAGEAEHDMLQCFIDSKYKDGRPTTESEVTGLLIAALFAGQHTSSITSTWTGAY 1037
            IIASRK AG++E+DMLQCFI+SKYKDGRPTTE+EVTGLLIAALFAGQHTSSITSTWTGAY
Sbjct: 240  IIASRKSAGKSENDMLQCFIESKYKDGRPTTEAEVTGLLIAALFAGQHTSSITSTWTGAY 299

Query: 1038 LLCNQKYMSAVQEEQKELMKKHGTKIDHDILSEMEVLYRCIKEALRLHPPLIMLLRQSHS 1217
            LL +++++SAV EEQK++M+KHG+K+DHDILSEM+ LYRCIKEALRLHPPLIMLLR SHS
Sbjct: 300  LLRHKEFLSAVIEEQKQIMQKHGSKVDHDILSEMDTLYRCIKEALRLHPPLIMLLRSSHS 359

Query: 1218 DFSVKTKDGKEYDIPKGHIVATSPAFANRLPHIYKDPDTYDPDRFSPGRDEDKAAGAFSY 1397
            DFSVKT+DGKEYDIPKGHIVATSPAFANRLP+IYKDPDTYDPDRFS GR+EDK AGAFSY
Sbjct: 360  DFSVKTRDGKEYDIPKGHIVATSPAFANRLPYIYKDPDTYDPDRFSVGREEDKVAGAFSY 419

Query: 1398 VSFGGGRHGCLGEPFAYLQIKAIWSHLLRNFEFELISPFPEIDWNAXXXXXXXXXXXRYK 1577
            +SFGGGRHGCLGEPFAYLQIKAIWSHLLRNFE EL+SPFPEIDWNA           RYK
Sbjct: 420  ISFGGGRHGCLGEPFAYLQIKAIWSHLLRNFELELVSPFPEIDWNAMVVGVKGKVMVRYK 479

Query: 1578 RRALPV 1595
            RR L V
Sbjct: 480  RRQLSV 485