BLASTX nr result
ID: Jatropha_contig00040000
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Jatropha_contig00040000
(376 letters)
Database: NCBI-nr (updated 2014/02/11)
35,149,712 sequences; 12,374,887,350 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002522014.1| DNA-directed RNA polymerase II, putative [Ri... 172 3e-41
ref|XP_002307132.1| predicted protein [Populus trichocarpa] gi|2... 172 4e-41
ref|XP_002282413.1| PREDICTED: DNA-directed RNA polymerase II su... 171 9e-41
gb|EOX92292.1| RNA polymerase II, Rpb4, core protein isoform 1 [... 170 1e-40
gb|ESR41250.1| hypothetical protein CICLE_v10026735mg [Citrus cl... 169 3e-40
gb|ESW06234.1| hypothetical protein PHAVU_010G030200g [Phaseolus... 169 4e-40
gb|AFK35928.1| unknown [Lotus japonicus] 169 4e-40
ref|NP_001236224.1| uncharacterized protein LOC100527704 [Glycin... 169 4e-40
ref|NP_001238073.1| uncharacterized protein LOC100527316 [Glycin... 169 4e-40
gb|EMJ07326.1| hypothetical protein PRUPE_ppa013142mg [Prunus pe... 168 6e-40
ref|NP_001237937.1| uncharacterized protein LOC100499661 [Glycin... 168 6e-40
ref|XP_004138390.1| PREDICTED: DNA-directed RNA polymerase II su... 168 7e-40
ref|XP_004509486.1| PREDICTED: DNA-directed RNA polymerase II su... 167 1e-39
ref|XP_006361114.1| PREDICTED: DNA-directed RNA polymerase II su... 167 2e-39
ref|XP_004241362.1| PREDICTED: DNA-directed RNA polymerase II su... 166 3e-39
gb|ESQ40930.1| hypothetical protein EUTSA_v10014976mg [Eutrema s... 166 4e-39
ref|XP_006288864.1| hypothetical protein CARUB_v10002229mg [Caps... 166 4e-39
ref|XP_003626907.1| DNA-directed RNA polymerase II subunit RPB4 ... 166 4e-39
ref|XP_004156236.1| PREDICTED: DNA-directed RNA polymerase II su... 165 6e-39
ref|XP_004141497.1| PREDICTED: DNA-directed RNA polymerase II su... 165 6e-39
>ref|XP_002522014.1| DNA-directed RNA polymerase II, putative [Ricinus communis]
gi|223538818|gb|EEF40418.1| DNA-directed RNA polymerase
II, putative [Ricinus communis]
Length = 138
Score = 172 bits (437), Expect = 3e-41
Identities = 87/90 (96%), Positives = 88/90 (97%)
Frame = +2
Query: 107 MSGEEEENASELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKFLQ 286
MSGEEEENASELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMS+DPMNQVSQVFEK LQ
Sbjct: 1 MSGEEEENASELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSNDPMNQVSQVFEKSLQ 60
Query: 287 YVKRFSRYKNPDAV*QVREILSRYQLAEFE 376
YVKRFSRYKNPDAV QVREILSRYQLAEFE
Sbjct: 61 YVKRFSRYKNPDAVRQVREILSRYQLAEFE 90
>ref|XP_002307132.1| predicted protein [Populus trichocarpa]
gi|224096326|ref|XP_002310604.1| predicted protein
[Populus trichocarpa] gi|222853507|gb|EEE91054.1|
hypothetical protein POPTR_0007s06660g [Populus
trichocarpa] gi|222856581|gb|EEE94128.1| hypothetical
protein POPTR_0005s08690g [Populus trichocarpa]
Length = 138
Score = 172 bits (436), Expect = 4e-41
Identities = 86/90 (95%), Positives = 88/90 (97%)
Frame = +2
Query: 107 MSGEEEENASELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKFLQ 286
MSGEEEENA+ELKIGD+FLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEK LQ
Sbjct: 1 MSGEEEENAAELKIGDDFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKSLQ 60
Query: 287 YVKRFSRYKNPDAV*QVREILSRYQLAEFE 376
YVKRFSRYKNPDAV QVREILSRYQLAEFE
Sbjct: 61 YVKRFSRYKNPDAVRQVREILSRYQLAEFE 90
>ref|XP_002282413.1| PREDICTED: DNA-directed RNA polymerase II subunit RPB4 [Vitis
vinifera] gi|147856183|emb|CAN80281.1| hypothetical
protein VITISV_017448 [Vitis vinifera]
gi|302143481|emb|CBI22042.3| unnamed protein product
[Vitis vinifera]
Length = 138
Score = 171 bits (433), Expect = 9e-41
Identities = 85/90 (94%), Positives = 88/90 (97%)
Frame = +2
Query: 107 MSGEEEENASELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKFLQ 286
MSGEEEENA+ELKIGDEFLKAKCLMNCEVA+ILEHKYEQLQQMSDDP+NQVSQVFEK LQ
Sbjct: 1 MSGEEEENAAELKIGDEFLKAKCLMNCEVAVILEHKYEQLQQMSDDPLNQVSQVFEKSLQ 60
Query: 287 YVKRFSRYKNPDAV*QVREILSRYQLAEFE 376
YVKRFSRYKNPDAV QVREILSRYQLAEFE
Sbjct: 61 YVKRFSRYKNPDAVRQVREILSRYQLAEFE 90
>gb|EOX92292.1| RNA polymerase II, Rpb4, core protein isoform 1 [Theobroma cacao]
gi|508700397|gb|EOX92293.1| RNA polymerase II, Rpb4,
core protein isoform 1 [Theobroma cacao]
Length = 138
Score = 170 bits (431), Expect = 1e-40
Identities = 85/90 (94%), Positives = 88/90 (97%)
Frame = +2
Query: 107 MSGEEEENASELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKFLQ 286
MSGEEEENA+ELKIG+EFLKAKCLMNCEVALILEHKYEQLQQMS+DPMNQVSQVFEK LQ
Sbjct: 1 MSGEEEENAAELKIGEEFLKAKCLMNCEVALILEHKYEQLQQMSEDPMNQVSQVFEKSLQ 60
Query: 287 YVKRFSRYKNPDAV*QVREILSRYQLAEFE 376
YVKRFSRYKNPDAV QVREILSRYQLAEFE
Sbjct: 61 YVKRFSRYKNPDAVRQVREILSRYQLAEFE 90
>gb|ESR41250.1| hypothetical protein CICLE_v10026735mg [Citrus clementina]
Length = 138
Score = 169 bits (428), Expect = 3e-40
Identities = 84/90 (93%), Positives = 87/90 (96%)
Frame = +2
Query: 107 MSGEEEENASELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKFLQ 286
MSGEEEENA+ELKIGDEFLKAKCLMNCEVA+IL+HKYEQLQQ SDDPMNQVSQVFEK LQ
Sbjct: 1 MSGEEEENAAELKIGDEFLKAKCLMNCEVAIILDHKYEQLQQTSDDPMNQVSQVFEKSLQ 60
Query: 287 YVKRFSRYKNPDAV*QVREILSRYQLAEFE 376
YVKRFSRYKNPDAV QVREILSRYQLAEFE
Sbjct: 61 YVKRFSRYKNPDAVRQVREILSRYQLAEFE 90
>gb|ESW06234.1| hypothetical protein PHAVU_010G030200g [Phaseolus vulgaris]
Length = 138
Score = 169 bits (427), Expect = 4e-40
Identities = 84/90 (93%), Positives = 87/90 (96%)
Frame = +2
Query: 107 MSGEEEENASELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKFLQ 286
MSGEEEENA+ELKIGDEFLKAKCLMNCEV+LILEHKYEQLQQ SDDPMNQVSQVFEK LQ
Sbjct: 1 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQTSDDPMNQVSQVFEKSLQ 60
Query: 287 YVKRFSRYKNPDAV*QVREILSRYQLAEFE 376
YVKRFSRYKNPDAV QVREIL+RYQLAEFE
Sbjct: 61 YVKRFSRYKNPDAVRQVREILARYQLAEFE 90
>gb|AFK35928.1| unknown [Lotus japonicus]
Length = 138
Score = 169 bits (427), Expect = 4e-40
Identities = 84/90 (93%), Positives = 87/90 (96%)
Frame = +2
Query: 107 MSGEEEENASELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKFLQ 286
MSGEEEENA+ELKIGDEFLKAKCLMNCEV+LILEHKYEQLQQ SDDPMNQVSQVFEK LQ
Sbjct: 1 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQSSDDPMNQVSQVFEKSLQ 60
Query: 287 YVKRFSRYKNPDAV*QVREILSRYQLAEFE 376
YVKRFSRYKNPDAV QVREIL+RYQLAEFE
Sbjct: 61 YVKRFSRYKNPDAVRQVREILARYQLAEFE 90
>ref|NP_001236224.1| uncharacterized protein LOC100527704 [Glycine max]
gi|255632992|gb|ACU16850.1| unknown [Glycine max]
Length = 195
Score = 169 bits (427), Expect = 4e-40
Identities = 84/90 (93%), Positives = 87/90 (96%)
Frame = +2
Query: 107 MSGEEEENASELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKFLQ 286
MSGEEEENA+ELKIGDEFLKAKCLMNCEVALILEHKYEQLQQ SDDPMNQVSQVFEK LQ
Sbjct: 1 MSGEEEENAAELKIGDEFLKAKCLMNCEVALILEHKYEQLQQTSDDPMNQVSQVFEKSLQ 60
Query: 287 YVKRFSRYKNPDAV*QVREILSRYQLAEFE 376
YVKRFSRYKNPDAV QVREIL+RY+LAEFE
Sbjct: 61 YVKRFSRYKNPDAVRQVREILARYELAEFE 90
>ref|NP_001238073.1| uncharacterized protein LOC100527316 [Glycine max]
gi|255632071|gb|ACU16388.1| unknown [Glycine max]
Length = 138
Score = 169 bits (427), Expect = 4e-40
Identities = 84/90 (93%), Positives = 87/90 (96%)
Frame = +2
Query: 107 MSGEEEENASELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKFLQ 286
MSGEEEENA+ELKIGDEFLKAKCLMNCEV+LILEHKYEQLQQ SDDPMNQVSQVFEK LQ
Sbjct: 1 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQTSDDPMNQVSQVFEKSLQ 60
Query: 287 YVKRFSRYKNPDAV*QVREILSRYQLAEFE 376
YVKRFSRYKNPDAV QVREIL+RYQLAEFE
Sbjct: 61 YVKRFSRYKNPDAVRQVREILARYQLAEFE 90
>gb|EMJ07326.1| hypothetical protein PRUPE_ppa013142mg [Prunus persica]
Length = 138
Score = 168 bits (426), Expect = 6e-40
Identities = 84/90 (93%), Positives = 86/90 (95%)
Frame = +2
Query: 107 MSGEEEENASELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKFLQ 286
MSGEEEENA+ELKIGDEFLKAKCLMNCEVALILEHKY+QLQQMSDDP NQVSQVFEK LQ
Sbjct: 1 MSGEEEENAAELKIGDEFLKAKCLMNCEVALILEHKYDQLQQMSDDPRNQVSQVFEKSLQ 60
Query: 287 YVKRFSRYKNPDAV*QVREILSRYQLAEFE 376
YVKRFSRYKNPDAV QVREILSRYQL EFE
Sbjct: 61 YVKRFSRYKNPDAVKQVREILSRYQLTEFE 90
>ref|NP_001237937.1| uncharacterized protein LOC100499661 [Glycine max]
gi|255625623|gb|ACU13156.1| unknown [Glycine max]
Length = 138
Score = 168 bits (426), Expect = 6e-40
Identities = 83/90 (92%), Positives = 87/90 (96%)
Frame = +2
Query: 107 MSGEEEENASELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKFLQ 286
MSGEEEENA+ELKIGDEFLKAKCLMNCEV+LILEHKYEQLQQ SDDPMNQ+SQVFEK LQ
Sbjct: 1 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQTSDDPMNQISQVFEKSLQ 60
Query: 287 YVKRFSRYKNPDAV*QVREILSRYQLAEFE 376
YVKRFSRYKNPDAV QVREIL+RYQLAEFE
Sbjct: 61 YVKRFSRYKNPDAVRQVREILARYQLAEFE 90
>ref|XP_004138390.1| PREDICTED: DNA-directed RNA polymerase II subunit RPB4-like
[Cucumis sativus] gi|449526195|ref|XP_004170099.1|
PREDICTED: DNA-directed RNA polymerase II subunit
RPB4-like [Cucumis sativus]
Length = 138
Score = 168 bits (425), Expect = 7e-40
Identities = 83/90 (92%), Positives = 87/90 (96%)
Frame = +2
Query: 107 MSGEEEENASELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKFLQ 286
MSGEEEENA+ELKIGDEFLKAKCLMNCEV+LILEHKYEQLQQMS+DP NQVSQVFEK LQ
Sbjct: 1 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQMSEDPTNQVSQVFEKSLQ 60
Query: 287 YVKRFSRYKNPDAV*QVREILSRYQLAEFE 376
YVKRFSRYKNPDAV QVRE+LSRYQLAEFE
Sbjct: 61 YVKRFSRYKNPDAVRQVREVLSRYQLAEFE 90
>ref|XP_004509486.1| PREDICTED: DNA-directed RNA polymerase II subunit RPB4-like [Cicer
arietinum] gi|502155876|ref|XP_004510223.1| PREDICTED:
DNA-directed RNA polymerase II subunit RPB4-like [Cicer
arietinum]
Length = 138
Score = 167 bits (424), Expect = 1e-39
Identities = 83/90 (92%), Positives = 87/90 (96%)
Frame = +2
Query: 107 MSGEEEENASELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKFLQ 286
MSGEEEENA+ELKIGDEFLKAKCLMNCEV+LIL+HKYEQLQQ SDDPMNQVSQVFEK LQ
Sbjct: 1 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILDHKYEQLQQSSDDPMNQVSQVFEKSLQ 60
Query: 287 YVKRFSRYKNPDAV*QVREILSRYQLAEFE 376
YVKRFSRYKNPDAV QVREIL+RYQLAEFE
Sbjct: 61 YVKRFSRYKNPDAVRQVREILARYQLAEFE 90
>ref|XP_006361114.1| PREDICTED: DNA-directed RNA polymerase II subunit 4-like [Solanum
tuberosum]
Length = 138
Score = 167 bits (422), Expect = 2e-39
Identities = 83/90 (92%), Positives = 85/90 (94%)
Frame = +2
Query: 107 MSGEEEENASELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKFLQ 286
MSGEEEENA+ELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSDDP NQ+SQVFEK LQ
Sbjct: 1 MSGEEEENAAELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSDDPTNQISQVFEKSLQ 60
Query: 287 YVKRFSRYKNPDAV*QVREILSRYQLAEFE 376
YVKRFSRYKNPDAV Q REILSRY LAEFE
Sbjct: 61 YVKRFSRYKNPDAVRQAREILSRYPLAEFE 90
>ref|XP_004241362.1| PREDICTED: DNA-directed RNA polymerase II subunit RPB4-like
[Solanum lycopersicum]
Length = 138
Score = 166 bits (420), Expect = 3e-39
Identities = 82/90 (91%), Positives = 85/90 (94%)
Frame = +2
Query: 107 MSGEEEENASELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKFLQ 286
MSGEEEENA+ELKIGDEFLKAKCLMNCEVALILEHKYEQ+QQMSDDP NQ+SQVFEK LQ
Sbjct: 1 MSGEEEENAAELKIGDEFLKAKCLMNCEVALILEHKYEQIQQMSDDPTNQISQVFEKSLQ 60
Query: 287 YVKRFSRYKNPDAV*QVREILSRYQLAEFE 376
YVKRFSRYKNPDAV Q REILSRY LAEFE
Sbjct: 61 YVKRFSRYKNPDAVRQAREILSRYPLAEFE 90
>gb|ESQ40930.1| hypothetical protein EUTSA_v10014976mg [Eutrema salsugineum]
Length = 138
Score = 166 bits (419), Expect = 4e-39
Identities = 82/90 (91%), Positives = 87/90 (96%)
Frame = +2
Query: 107 MSGEEEENASELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKFLQ 286
MSGEEEENA+ELKIGDEFLKAKCLMNCEV+LILEHKYEQLQQ+S+DPMNQVSQVFEK LQ
Sbjct: 1 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQVSEDPMNQVSQVFEKSLQ 60
Query: 287 YVKRFSRYKNPDAV*QVREILSRYQLAEFE 376
YVKRFSRYKNPDAV QVREILSR+QL EFE
Sbjct: 61 YVKRFSRYKNPDAVRQVREILSRHQLTEFE 90
>ref|XP_006288864.1| hypothetical protein CARUB_v10002229mg [Capsella rubella]
gi|482557570|gb|EOA21762.1| hypothetical protein
CARUB_v10002229mg [Capsella rubella]
Length = 138
Score = 166 bits (419), Expect = 4e-39
Identities = 82/90 (91%), Positives = 87/90 (96%)
Frame = +2
Query: 107 MSGEEEENASELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKFLQ 286
MSGEEEENA+ELKIGDEFLKAKCLMNCEV+LILEHKYEQLQQ+S+DPMNQVSQVFEK LQ
Sbjct: 1 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQVSEDPMNQVSQVFEKSLQ 60
Query: 287 YVKRFSRYKNPDAV*QVREILSRYQLAEFE 376
YVKRFSRYKNPDAV QVREILSR+QL EFE
Sbjct: 61 YVKRFSRYKNPDAVRQVREILSRHQLTEFE 90
>ref|XP_003626907.1| DNA-directed RNA polymerase II subunit RPB4 [Medicago truncatula]
gi|355520929|gb|AET01383.1| DNA-directed RNA polymerase
II subunit RPB4 [Medicago truncatula]
gi|388497296|gb|AFK36714.1| unknown [Medicago
truncatula]
Length = 138
Score = 166 bits (419), Expect = 4e-39
Identities = 83/90 (92%), Positives = 86/90 (95%)
Frame = +2
Query: 107 MSGEEEENASELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKFLQ 286
MSGEEEENA+ELKIGDEFLKAKCLMNCEV+LILEHKYEQLQQ SDD MNQVSQVFEK LQ
Sbjct: 1 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQSSDDAMNQVSQVFEKSLQ 60
Query: 287 YVKRFSRYKNPDAV*QVREILSRYQLAEFE 376
YVKRFSRYKNPDAV QVREIL+RYQLAEFE
Sbjct: 61 YVKRFSRYKNPDAVRQVREILARYQLAEFE 90
>ref|XP_004156236.1| PREDICTED: DNA-directed RNA polymerase II subunit RPB4-like
[Cucumis sativus]
Length = 139
Score = 165 bits (417), Expect = 6e-39
Identities = 82/90 (91%), Positives = 86/90 (95%)
Frame = +2
Query: 107 MSGEEEENASELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKFLQ 286
MSGEEEENA+ELKIGDEFLKAKCLMNCEV+LILEHKYEQLQQ ++DP NQVSQVFEK LQ
Sbjct: 1 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
Query: 287 YVKRFSRYKNPDAV*QVREILSRYQLAEFE 376
YVKRFSRYKNPDAV QVREILSRYQLAEFE
Sbjct: 61 YVKRFSRYKNPDAVRQVREILSRYQLAEFE 90
>ref|XP_004141497.1| PREDICTED: DNA-directed RNA polymerase II subunit RPB4-like
[Cucumis sativus]
Length = 138
Score = 165 bits (417), Expect = 6e-39
Identities = 82/90 (91%), Positives = 86/90 (95%)
Frame = +2
Query: 107 MSGEEEENASELKIGDEFLKAKCLMNCEVALILEHKYEQLQQMSDDPMNQVSQVFEKFLQ 286
MSGEEEENA+ELKIGDEFLKAKCLMNCEV+LILEHKYEQLQQ ++DP NQVSQVFEK LQ
Sbjct: 1 MSGEEEENAAELKIGDEFLKAKCLMNCEVSLILEHKYEQLQQRAEDPTNQVSQVFEKSLQ 60
Query: 287 YVKRFSRYKNPDAV*QVREILSRYQLAEFE 376
YVKRFSRYKNPDAV QVREILSRYQLAEFE
Sbjct: 61 YVKRFSRYKNPDAVRQVREILSRYQLAEFE 90
Database: NCBI-nr (updated 2014/02/11)
Posted date: Feb 11, 2014 4:56 AM
Number of letters in database: 12,374,887,350
Number of sequences in database: 35,149,712
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 35149712
Number of Hits to DB: 57,283,302,026,007
Number of extensions: 321264217
Number of successful extensions: -1346775739
Number of sequences better than 1.0e-05: 8440526
Number of HSP's gapped: -1368198042
Number of HSP's successfully gapped: 10267991
Length of database: 12,374,887,350
Neighboring words threshold: 12
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 30 (16.2 bits)