BLASTX nr result
ID: Jatropha_contig00010869
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Jatropha_contig00010869
(550 letters)
Database: NCBI-nr (updated 2014/02/11)
35,149,712 sequences; 12,374,887,350 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_002530090.1| conserved hypothetical protein [Ricinus comm... 187 2e-45
gb|EOY09097.1| Dehydration-induced protein, putative [Theobroma ... 184 1e-44
ref|XP_002307999.1| predicted protein [Populus trichocarpa] gi|2... 182 6e-44
gb|ABK93250.1| unknown [Populus trichocarpa] 180 2e-43
gb|ERP51522.1| hypothetical protein POPTR_0016s04110g [Populus t... 175 5e-42
ref|XP_002322645.1| predicted protein [Populus trichocarpa] gi|2... 175 5e-42
gb|ESR34466.1| hypothetical protein CICLE_v10006124mg [Citrus cl... 174 1e-41
gb|ESR34464.1| hypothetical protein CICLE_v10006124mg [Citrus cl... 174 1e-41
ref|XP_004303625.1| PREDICTED: protein EARLY RESPONSIVE TO DEHYD... 160 2e-37
ref|XP_002276510.1| PREDICTED: polyadenylate-binding protein-int... 159 4e-37
gb|ESW34138.1| hypothetical protein PHAVU_001G127900g [Phaseolus... 155 7e-36
gb|EMJ16312.1| hypothetical protein PRUPE_ppa012883mg [Prunus pe... 155 7e-36
gb|EMJ16311.1| hypothetical protein PRUPE_ppa012883mg [Prunus pe... 155 7e-36
gb|AAZ20289.1| unknown [Arachis hypogaea] 151 1e-34
ref|XP_003625072.1| Polyadenylate-binding protein-interacting pr... 149 5e-34
ref|XP_003521157.1| PREDICTED: polyadenylate-binding protein-int... 148 9e-34
gb|ACU19169.1| unknown [Glycine max] 145 8e-33
gb|AFK33641.1| unknown [Lotus japonicus] 143 2e-32
ref|XP_004493438.1| PREDICTED: protein EARLY RESPONSIVE TO DEHYD... 142 4e-32
dbj|BAJ34324.1| unnamed protein product [Thellungiella halophila... 142 7e-32
>ref|XP_002530090.1| conserved hypothetical protein [Ricinus communis]
gi|223530401|gb|EEF32289.1| conserved hypothetical
protein [Ricinus communis]
Length = 136
Score = 187 bits (474), Expect = 2e-45
Identities = 87/103 (84%), Positives = 94/103 (91%), Gaps = 1/103 (0%)
Frame = +1
Query: 115 MALVSGGRSTLNPDAPLFIPAAYQQVEDFSPEWWQLVTTSTWYRDYWLSQHQDEEGFYDN 294
MALVSGGRSTLNPDAPLFIPAAY+QVEDFSPEWWQLVT STWYRDYWLSQHQDE+GFYDN
Sbjct: 1 MALVSGGRSTLNPDAPLFIPAAYRQVEDFSPEWWQLVTNSTWYRDYWLSQHQDEDGFYDN 60
Query: 295 AEDD-SYDACDVADLLPDTFDLDAGEDFSTLEVQFQEFVESYQ 420
AEDD +D DV DLLP+TFDLDAGE FS+LE QF EFV+SY+
Sbjct: 61 AEDDEGFDGNDVVDLLPETFDLDAGEYFSSLEAQFHEFVDSYE 103
>gb|EOY09097.1| Dehydration-induced protein, putative [Theobroma cacao]
Length = 166
Score = 184 bits (467), Expect = 1e-44
Identities = 81/102 (79%), Positives = 94/102 (92%)
Frame = +1
Query: 115 MALVSGGRSTLNPDAPLFIPAAYQQVEDFSPEWWQLVTTSTWYRDYWLSQHQDEEGFYDN 294
MALV+GGRSTLNP+APLFIPA Y+QVEDFSPEWWQLVTTSTWYRDYWLSQHQDE+GFYDN
Sbjct: 1 MALVAGGRSTLNPNAPLFIPAVYRQVEDFSPEWWQLVTTSTWYRDYWLSQHQDEDGFYDN 60
Query: 295 AEDDSYDACDVADLLPDTFDLDAGEDFSTLEVQFQEFVESYQ 420
AEDD +D D+ADLLPDTFDL A ED S +++QF+EF++SY+
Sbjct: 61 AEDDGFDGNDIADLLPDTFDLVADEDLSGMDLQFEEFIQSYE 102
>ref|XP_002307999.1| predicted protein [Populus trichocarpa] gi|222853975|gb|EEE91522.1|
LIGHT STRESS-REGULATED 1 family protein [Populus
trichocarpa]
Length = 143
Score = 182 bits (461), Expect = 6e-44
Identities = 83/103 (80%), Positives = 95/103 (92%), Gaps = 1/103 (0%)
Frame = +1
Query: 106 LKQMALVSGGRSTLNPDAPLFIPAAYQQVEDFSPEWWQLVTTSTWYRDYWLSQHQDEEGF 285
LK+MALVSGGRSTLNPDAPLF+PAAY+QVEDFSPEWWQLVTT+TW+RDYWLSQHQDE GF
Sbjct: 8 LKRMALVSGGRSTLNPDAPLFVPAAYRQVEDFSPEWWQLVTTTTWFRDYWLSQHQDENGF 67
Query: 286 YDNAEDD-SYDACDVADLLPDTFDLDAGEDFSTLEVQFQEFVE 411
YDNAE+D +D DVADLLPDTFDLDAG+ F++LE QF +F+E
Sbjct: 68 YDNAENDFGFDGNDVADLLPDTFDLDAGDYFTSLEAQFADFIE 110
>gb|ABK93250.1| unknown [Populus trichocarpa]
Length = 143
Score = 180 bits (456), Expect = 2e-43
Identities = 82/103 (79%), Positives = 95/103 (92%), Gaps = 1/103 (0%)
Frame = +1
Query: 106 LKQMALVSGGRSTLNPDAPLFIPAAYQQVEDFSPEWWQLVTTSTWYRDYWLSQHQDEEGF 285
LK+MALVSGGRSTLNPDAPLF+PAAY+QVEDFSPEWWQLVTT+TW+RDYWLSQHQDE GF
Sbjct: 8 LKRMALVSGGRSTLNPDAPLFVPAAYRQVEDFSPEWWQLVTTTTWFRDYWLSQHQDENGF 67
Query: 286 YDNAEDD-SYDACDVADLLPDTFDLDAGEDFSTLEVQFQEFVE 411
YDNAE+D +D DVADLLP+TFDLDAG+ F++LE QF +F+E
Sbjct: 68 YDNAENDFGFDGNDVADLLPNTFDLDAGDYFTSLEAQFADFIE 110
>gb|ERP51522.1| hypothetical protein POPTR_0016s04110g [Populus trichocarpa]
Length = 141
Score = 175 bits (444), Expect = 5e-42
Identities = 82/103 (79%), Positives = 92/103 (89%), Gaps = 1/103 (0%)
Frame = +1
Query: 106 LKQMALVSGGRSTLNPDAPLFIPAAYQQVEDFSPEWWQLVTTSTWYRDYWLSQHQDEEGF 285
LK+MALVSGGRSTLNPDAPLF+PAAY+QVEDFSPEWWQLVTT+TW+RDYWLSQHQDE GF
Sbjct: 22 LKRMALVSGGRSTLNPDAPLFVPAAYRQVEDFSPEWWQLVTTTTWFRDYWLSQHQDENGF 81
Query: 286 YDNAEDD-SYDACDVADLLPDTFDLDAGEDFSTLEVQFQEFVE 411
YDNAED+ D +VADLLPDTFDLDAG+ FS+L+ QF F E
Sbjct: 82 YDNAEDEFGLDGNNVADLLPDTFDLDAGDYFSSLDSQFAGFAE 124
>ref|XP_002322645.1| predicted protein [Populus trichocarpa] gi|222867275|gb|EEF04406.1|
LIGHT STRESS-REGULATED 1 family protein [Populus
trichocarpa]
Length = 157
Score = 175 bits (444), Expect = 5e-42
Identities = 82/103 (79%), Positives = 92/103 (89%), Gaps = 1/103 (0%)
Frame = +1
Query: 106 LKQMALVSGGRSTLNPDAPLFIPAAYQQVEDFSPEWWQLVTTSTWYRDYWLSQHQDEEGF 285
LK+MALVSGGRSTLNPDAPLF+PAAY+QVEDFSPEWWQLVTT+TW+RDYWLSQHQDE GF
Sbjct: 22 LKRMALVSGGRSTLNPDAPLFVPAAYRQVEDFSPEWWQLVTTTTWFRDYWLSQHQDENGF 81
Query: 286 YDNAEDD-SYDACDVADLLPDTFDLDAGEDFSTLEVQFQEFVE 411
YDNAED+ D +VADLLPDTFDLDAG+ FS+L+ QF F E
Sbjct: 82 YDNAEDEFGLDGNNVADLLPDTFDLDAGDYFSSLDSQFAGFAE 124
>gb|ESR34466.1| hypothetical protein CICLE_v10006124mg [Citrus clementina]
Length = 121
Score = 174 bits (441), Expect = 1e-41
Identities = 79/102 (77%), Positives = 92/102 (90%)
Frame = +1
Query: 115 MALVSGGRSTLNPDAPLFIPAAYQQVEDFSPEWWQLVTTSTWYRDYWLSQHQDEEGFYDN 294
MALVSG RSTLNP+APLFIPAA++QVEDFSPEWWQL+TTSTWY +YWLSQHQDE+GFYDN
Sbjct: 1 MALVSG-RSTLNPNAPLFIPAAFRQVEDFSPEWWQLITTSTWYHNYWLSQHQDEDGFYDN 59
Query: 295 AEDDSYDACDVADLLPDTFDLDAGEDFSTLEVQFQEFVESYQ 420
DD +D +VADLLPDTFDLDAGE+FS++E QF EFV S++
Sbjct: 60 GGDDEFDVSNVADLLPDTFDLDAGENFSSIEAQFDEFVMSFE 101
>gb|ESR34464.1| hypothetical protein CICLE_v10006124mg [Citrus clementina]
gi|557523098|gb|ESR34465.1| hypothetical protein
CICLE_v10006124mg [Citrus clementina]
Length = 156
Score = 174 bits (441), Expect = 1e-41
Identities = 79/102 (77%), Positives = 92/102 (90%)
Frame = +1
Query: 115 MALVSGGRSTLNPDAPLFIPAAYQQVEDFSPEWWQLVTTSTWYRDYWLSQHQDEEGFYDN 294
MALVSG RSTLNP+APLFIPAA++QVEDFSPEWWQL+TTSTWY +YWLSQHQDE+GFYDN
Sbjct: 1 MALVSG-RSTLNPNAPLFIPAAFRQVEDFSPEWWQLITTSTWYHNYWLSQHQDEDGFYDN 59
Query: 295 AEDDSYDACDVADLLPDTFDLDAGEDFSTLEVQFQEFVESYQ 420
DD +D +VADLLPDTFDLDAGE+FS++E QF EFV S++
Sbjct: 60 GGDDEFDVSNVADLLPDTFDLDAGENFSSIEAQFDEFVMSFE 101
>ref|XP_004303625.1| PREDICTED: protein EARLY RESPONSIVE TO DEHYDRATION 15-like
[Fragaria vesca subsp. vesca]
Length = 154
Score = 160 bits (404), Expect = 2e-37
Identities = 81/103 (78%), Positives = 91/103 (88%), Gaps = 1/103 (0%)
Frame = +1
Query: 115 MALVSGGRSTLNPDAPLFIPAAYQQVEDFSPEWWQLVTTSTWYRDYWLSQHQDEEGFYDN 294
MALVSG RSTLNP+APLFIPAA +QVEDFSPEWWQLVTTSTWY DYWLSQ+QDE+GFYDN
Sbjct: 1 MALVSG-RSTLNPNAPLFIPAALRQVEDFSPEWWQLVTTSTWYHDYWLSQNQDEDGFYDN 59
Query: 295 AEDDSYDACDVADLLPDTFDLDAGEDFSTLE-VQFQEFVESYQ 420
A D+YD +VADLLP+TFDLDAGEDFS E QF+EF++S Q
Sbjct: 60 AH-DNYD--NVADLLPETFDLDAGEDFSNFEAAQFEEFLQSTQ 99
>ref|XP_002276510.1| PREDICTED: polyadenylate-binding protein-interacting protein 2
[Vitis vinifera] gi|147782754|emb|CAN70573.1|
hypothetical protein VITISV_027129 [Vitis vinifera]
Length = 169
Score = 159 bits (402), Expect = 4e-37
Identities = 71/100 (71%), Positives = 85/100 (85%)
Frame = +1
Query: 115 MALVSGGRSTLNPDAPLFIPAAYQQVEDFSPEWWQLVTTSTWYRDYWLSQHQDEEGFYDN 294
MALV+GGRSTLNP+APLFIPAA++QVEDFSPEWWQL+TTSTW+RDYWLSQHQ+ + F N
Sbjct: 1 MALVTGGRSTLNPNAPLFIPAAFRQVEDFSPEWWQLITTSTWFRDYWLSQHQEGDIFNCN 60
Query: 295 AEDDSYDACDVADLLPDTFDLDAGEDFSTLEVQFQEFVES 414
DD +DA D+ADLLPD FD EDFS +E QF+EF++S
Sbjct: 61 NADDEFDANDIADLLPDAFDFGTEEDFSHMEAQFEEFIQS 100
>gb|ESW34138.1| hypothetical protein PHAVU_001G127900g [Phaseolus vulgaris]
Length = 110
Score = 155 bits (391), Expect = 7e-36
Identities = 68/102 (66%), Positives = 86/102 (84%)
Frame = +1
Query: 115 MALVSGGRSTLNPDAPLFIPAAYQQVEDFSPEWWQLVTTSTWYRDYWLSQHQDEEGFYDN 294
MALVSGGRSTLNP+APL+IPAA++QVEDFSPEWWQLVTT TWY D+WLSQ Q++ +Y
Sbjct: 1 MALVSGGRSTLNPNAPLYIPAAFRQVEDFSPEWWQLVTTFTWYHDFWLSQQQEDGAYY-- 58
Query: 295 AEDDSYDACDVADLLPDTFDLDAGEDFSTLEVQFQEFVESYQ 420
E+D +D DV DLLPD+FDLDAGED + L+ Q ++F++SY+
Sbjct: 59 GEEDEFDGNDVVDLLPDSFDLDAGEDLAALDAQLEDFIQSYE 100
>gb|EMJ16312.1| hypothetical protein PRUPE_ppa012883mg [Prunus persica]
Length = 150
Score = 155 bits (391), Expect = 7e-36
Identities = 78/102 (76%), Positives = 88/102 (86%)
Frame = +1
Query: 115 MALVSGGRSTLNPDAPLFIPAAYQQVEDFSPEWWQLVTTSTWYRDYWLSQHQDEEGFYDN 294
MALVSGG STLNP+APLFIPAA +QVEDFSPEWWQLVTTSTWY DYWLSQ Q E+GFYDN
Sbjct: 1 MALVSGG-STLNPNAPLFIPAALKQVEDFSPEWWQLVTTSTWYHDYWLSQ-QGEDGFYDN 58
Query: 295 AEDDSYDACDVADLLPDTFDLDAGEDFSTLEVQFQEFVESYQ 420
A+ D +VADLLP+TFDLDAGEDFS+LE QF+EF++ Q
Sbjct: 59 AQG---DVDNVADLLPETFDLDAGEDFSSLEAQFEEFLQLSQ 97
>gb|EMJ16311.1| hypothetical protein PRUPE_ppa012883mg [Prunus persica]
Length = 111
Score = 155 bits (391), Expect = 7e-36
Identities = 78/102 (76%), Positives = 88/102 (86%)
Frame = +1
Query: 115 MALVSGGRSTLNPDAPLFIPAAYQQVEDFSPEWWQLVTTSTWYRDYWLSQHQDEEGFYDN 294
MALVSGG STLNP+APLFIPAA +QVEDFSPEWWQLVTTSTWY DYWLSQ Q E+GFYDN
Sbjct: 1 MALVSGG-STLNPNAPLFIPAALKQVEDFSPEWWQLVTTSTWYHDYWLSQ-QGEDGFYDN 58
Query: 295 AEDDSYDACDVADLLPDTFDLDAGEDFSTLEVQFQEFVESYQ 420
A+ D +VADLLP+TFDLDAGEDFS+LE QF+EF++ Q
Sbjct: 59 AQG---DVDNVADLLPETFDLDAGEDFSSLEAQFEEFLQLSQ 97
>gb|AAZ20289.1| unknown [Arachis hypogaea]
Length = 124
Score = 151 bits (381), Expect = 1e-34
Identities = 76/111 (68%), Positives = 88/111 (79%), Gaps = 3/111 (2%)
Frame = +1
Query: 115 MALVSGGRSTLNPDAPLFIPAAYQQVEDFSPEWWQLVTTSTWYRDYWLSQHQDEEGFYDN 294
MALVSGGR TLNP+APL+IPAA++QVEDFSPEWWQLVTT TWY +YWLSQ Q E GFY
Sbjct: 1 MALVSGGRPTLNPNAPLYIPAAFRQVEDFSPEWWQLVTTLTWYHEYWLSQQQVEGGFY-A 59
Query: 295 AEDDSY--DACDVADLLPDTFDLDAGEDFSTLEVQFQEFVE-SYQLM*IAD 438
AEDD + D DV DLLPD FDLDAGED S +E Q +EF+ S +L+ IA+
Sbjct: 60 AEDDGFDQDGNDVVDLLPDIFDLDAGEDLSVMEAQLEEFIRLSEELLMIAE 110
>ref|XP_003625072.1| Polyadenylate-binding protein-interacting protein [Medicago
truncatula] gi|87241447|gb|ABD33305.1| hypothetical
protein MtrDRAFT_AC158502g14v2 [Medicago truncatula]
gi|355500087|gb|AES81290.1| Polyadenylate-binding
protein-interacting protein [Medicago truncatula]
gi|388513539|gb|AFK44831.1| unknown [Medicago
truncatula]
Length = 115
Score = 149 bits (375), Expect = 5e-34
Identities = 70/100 (70%), Positives = 81/100 (81%)
Frame = +1
Query: 115 MALVSGGRSTLNPDAPLFIPAAYQQVEDFSPEWWQLVTTSTWYRDYWLSQHQDEEGFYDN 294
MALVSGGRSTLNP+APL+IPAA++ VEDFSPEWWQLVTT WY DYWLSQ D+EGFYD+
Sbjct: 1 MALVSGGRSTLNPNAPLYIPAAFRYVEDFSPEWWQLVTTLKWYHDYWLSQQHDDEGFYDD 60
Query: 295 AEDDSYDACDVADLLPDTFDLDAGEDFSTLEVQFQEFVES 414
D +D DV DLLPD FDLDA ED S +E Q +EF++S
Sbjct: 61 V--DEFDGNDVVDLLPDAFDLDADEDIS-IEAQLEEFIKS 97
>ref|XP_003521157.1| PREDICTED: polyadenylate-binding protein-interacting protein 2-like
[Glycine max]
Length = 105
Score = 148 bits (373), Expect = 9e-34
Identities = 71/100 (71%), Positives = 79/100 (79%)
Frame = +1
Query: 115 MALVSGGRSTLNPDAPLFIPAAYQQVEDFSPEWWQLVTTSTWYRDYWLSQHQDEEGFYDN 294
MALVSGGRSTLNP+APL+IPAA++QVEDFSPEWWQLVTT TWY DYWLSQ DE FY
Sbjct: 1 MALVSGGRSTLNPNAPLYIPAAFRQVEDFSPEWWQLVTTLTWYHDYWLSQQHDEGAFY-- 58
Query: 295 AEDDSYDACDVADLLPDTFDLDAGEDFSTLEVQFQEFVES 414
EDD +D DV DLLPD+FDLDAGED F+EF+ S
Sbjct: 59 GEDDGFDGNDVVDLLPDSFDLDAGEDL------FEEFIRS 92
>gb|ACU19169.1| unknown [Glycine max]
Length = 105
Score = 145 bits (365), Expect = 8e-33
Identities = 69/99 (69%), Positives = 77/99 (77%)
Frame = +1
Query: 115 MALVSGGRSTLNPDAPLFIPAAYQQVEDFSPEWWQLVTTSTWYRDYWLSQHQDEEGFYDN 294
MALVSGGRSTLNP+APL+IPAA++QVEDF PEWWQLVTT TWY DYWLSQ DE FY
Sbjct: 1 MALVSGGRSTLNPNAPLYIPAAFRQVEDFFPEWWQLVTTLTWYHDYWLSQQHDEGAFY-- 58
Query: 295 AEDDSYDACDVADLLPDTFDLDAGEDFSTLEVQFQEFVE 411
EDD +D DV DLLPD+FDLDAGED ++F E E
Sbjct: 59 GEDDGFDGNDVVDLLPDSFDLDAGEDLFEEFIRFSESQE 97
>gb|AFK33641.1| unknown [Lotus japonicus]
Length = 103
Score = 143 bits (361), Expect = 2e-32
Identities = 69/103 (66%), Positives = 79/103 (76%), Gaps = 3/103 (2%)
Frame = +1
Query: 115 MALVSGGRSTLNPDAPLFIPAAYQQVEDFSPEWWQLVTTSTWYRDYWLSQHQDEEGFYDN 294
MALVSG RSTLNP+APL+IPAA++QVEDFSPEWWQLVTT TWY D+WLSQ D+ FY N
Sbjct: 1 MALVSGARSTLNPNAPLYIPAAFRQVEDFSPEWWQLVTTLTWYHDFWLSQQHDDGAFYGN 60
Query: 295 ---AEDDSYDACDVADLLPDTFDLDAGEDFSTLEVQFQEFVES 414
ED DV DLLPD+FDLDAGED S +E Q +EF+ S
Sbjct: 61 NVVGED------DVLDLLPDSFDLDAGEDLSVMEAQLEEFIRS 97
>ref|XP_004493438.1| PREDICTED: protein EARLY RESPONSIVE TO DEHYDRATION 15-like [Cicer
arietinum]
Length = 102
Score = 142 bits (359), Expect = 4e-32
Identities = 70/100 (70%), Positives = 79/100 (79%)
Frame = +1
Query: 115 MALVSGGRSTLNPDAPLFIPAAYQQVEDFSPEWWQLVTTSTWYRDYWLSQHQDEEGFYDN 294
MALVSGGRSTLNP+APLFIPAA+ QVEDFSPEWWQLVTT TWY D+WLSQ D+EGFY
Sbjct: 1 MALVSGGRSTLNPNAPLFIPAAFHQVEDFSPEWWQLVTTLTWYHDFWLSQQHDDEGFYH- 59
Query: 295 AEDDSYDACDVADLLPDTFDLDAGEDFSTLEVQFQEFVES 414
ED DV DLLPD FDLD +DFS +E Q +EF++S
Sbjct: 60 -ED------DVVDLLPDAFDLDVADDFS-IEAQLEEFIKS 91
>dbj|BAJ34324.1| unnamed protein product [Thellungiella halophila]
gi|557112577|gb|ESQ52861.1| hypothetical protein
EUTSA_v10017353mg [Eutrema salsugineum]
gi|557112578|gb|ESQ52862.1| hypothetical protein
EUTSA_v10017353mg [Eutrema salsugineum]
Length = 159
Score = 142 bits (357), Expect = 7e-32
Identities = 67/102 (65%), Positives = 79/102 (77%), Gaps = 1/102 (0%)
Frame = +1
Query: 115 MALVSGGRSTLNPDAPLFIPAAYQQVEDFSPEWWQLVTTSTWYRDYWLSQHQDEEGFYDN 294
MA+VSG RSTLNPDAPLF+PAA +QVEDFSPEWWQLVTTSTWY DYW+SQHQ +GFYDN
Sbjct: 1 MAMVSGRRSTLNPDAPLFVPAAVRQVEDFSPEWWQLVTTSTWYHDYWISQHQGPDGFYDN 60
Query: 295 AEDDSYDA-CDVADLLPDTFDLDAGEDFSTLEVQFQEFVESY 417
E+++ DVADLLP++FD D EDF ++ EF Y
Sbjct: 61 GENENRGGEVDVADLLPESFDFDDMEDF--FDIDAAEFDHGY 100