BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Jatropha_contig00006111
         (414 letters)

Database: NCBI-nr (updated 2014/02/11) 
           35,149,712 sequences; 12,374,887,350 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_002518910.1| cystathionine gamma-synthase, putative [Rici...   177   1e-42
gb|EOY23895.1| Methionine gamma-lyase [Theobroma cacao]               172   4e-41
gb|ESW33579.1| hypothetical protein PHAVU_001G082000g [Phaseolus...   169   4e-40
ref|XP_004309539.1| PREDICTED: methionine gamma-lyase-like [Frag...   168   6e-40
gb|EMJ10322.1| hypothetical protein PRUPE_ppa005470mg [Prunus pe...   167   1e-39
ref|XP_003536171.1| PREDICTED: methionine gamma-lyase-like [Glyc...   167   1e-39
gb|ESR52463.1| hypothetical protein CICLE_v10020136mg [Citrus cl...   166   2e-39
ref|XP_002299428.1| predicted protein [Populus trichocarpa] gi|2...   165   5e-39
gb|ABK96059.1| unknown [Populus trichocarpa]                          165   5e-39
ref|XP_004495508.1| PREDICTED: methionine gamma-lyase-like [Cice...   160   2e-37
ref|XP_006343784.1| PREDICTED: methionine gamma-lyase-like [Sola...   160   2e-37
gb|EPS64325.1| hypothetical protein M569_10456 [Genlisea aurea]       157   2e-36
ref|XP_004245457.1| PREDICTED: methionine gamma-lyase-like [Sola...   155   5e-36
ref|XP_004244654.1| PREDICTED: methionine gamma-lyase-like [Sola...   155   5e-36
gb|AGF70153.1| methionine gamma-lyase [Cucumis melo]                  155   6e-36
gb|ADN33936.1| cystathionine gamma-synthase [Cucumis melo subsp....   155   6e-36
ref|XP_002280162.1| PREDICTED: methionine gamma-lyase [Vitis vin...   152   3e-35
gb|ABK27101.1| unknown [Picea sitchensis]                             152   5e-35
emb|CAN83499.1| hypothetical protein VITISV_026969 [Vitis vinifera]   150   1e-34
ref|XP_006352296.1| PREDICTED: methionine gamma-lyase-like [Sola...   150   2e-34

>ref|XP_002518910.1| cystathionine gamma-synthase, putative [Ricinus communis]
           gi|223541897|gb|EEF43443.1| cystathionine
           gamma-synthase, putative [Ricinus communis]
          Length = 449

 Score =  177 bits (449), Expect = 1e-42
 Identities = 86/113 (76%), Positives = 95/113 (84%)
 Frame = -2

Query: 389 LEEHPQHQLLKSMANKDYGFSALVCLDMETEERANRLMNHLQNYTQFGLMAVSLGYYETL 210
           LE+HP HQLLKSMANKDYGF AL+CLDMETEE+ANRL+NHLQNYTQFG MAVSLGYYETL
Sbjct: 335 LEDHPHHQLLKSMANKDYGFGALLCLDMETEEKANRLLNHLQNYTQFGFMAVSLGYYETL 394

Query: 209 MXXXXXXXXXXXXXXEKKLAGISPGLIRMSIGYIGNLEQKWSQFEKAISRMQD 51
           M              EKKLAGISPGLIRMS+GY+G LEQKWSQFEKA+SRM++
Sbjct: 395 MSVSGSSTSSELNAEEKKLAGISPGLIRMSVGYLGTLEQKWSQFEKALSRMKE 447


>gb|EOY23895.1| Methionine gamma-lyase [Theobroma cacao]
          Length = 454

 Score =  172 bits (436), Expect = 4e-41
 Identities = 84/115 (73%), Positives = 96/115 (83%)
 Frame = -2

Query: 389 LEEHPQHQLLKSMANKDYGFSALVCLDMETEERANRLMNHLQNYTQFGLMAVSLGYYETL 210
           LE+HPQH+LLKSMANK+YGF  L+C+DME+EERANRLM+HLQNYTQFGLMAVSLGYYETL
Sbjct: 335 LEDHPQHKLLKSMANKEYGFGGLLCVDMESEERANRLMHHLQNYTQFGLMAVSLGYYETL 394

Query: 209 MXXXXXXXXXXXXXXEKKLAGISPGLIRMSIGYIGNLEQKWSQFEKAISRMQDSS 45
           M              EK LAGISPGL+RMSIGYIG LEQ+WSQ EKA+SRMQ+S+
Sbjct: 395 MSCSGSSTSSELNAEEKALAGISPGLVRMSIGYIGTLEQRWSQLEKALSRMQEST 449


>gb|ESW33579.1| hypothetical protein PHAVU_001G082000g [Phaseolus vulgaris]
          Length = 439

 Score =  169 bits (427), Expect = 4e-40
 Identities = 82/116 (70%), Positives = 92/116 (79%)
 Frame = -2

Query: 389 LEEHPQHQLLKSMANKDYGFSALVCLDMETEERANRLMNHLQNYTQFGLMAVSLGYYETL 210
           LEEHPQHQLLKSM N +YGF  L+C+DMETEERANRLMNHLQNY QFG MAVSLGYYETL
Sbjct: 322 LEEHPQHQLLKSMHNAEYGFGGLICVDMETEERANRLMNHLQNYGQFGFMAVSLGYYETL 381

Query: 209 MXXXXXXXXXXXXXXEKKLAGISPGLIRMSIGYIGNLEQKWSQFEKAISRMQDSSF 42
           M              EKKLAGISPGL+RMSIGYIG LEQKW+Q +KA++R+Q+  F
Sbjct: 382 MSCSGSSTSSELNSEEKKLAGISPGLVRMSIGYIGTLEQKWAQLDKALTRLQEPPF 437


>ref|XP_004309539.1| PREDICTED: methionine gamma-lyase-like [Fragaria vesca subsp.
           vesca]
          Length = 455

 Score =  168 bits (426), Expect = 6e-40
 Identities = 84/114 (73%), Positives = 91/114 (79%)
 Frame = -2

Query: 389 LEEHPQHQLLKSMANKDYGFSALVCLDMETEERANRLMNHLQNYTQFGLMAVSLGYYETL 210
           LEEHPQH+LLKSM NKDYG+  L+CLDM TEERANRLMN LQNYTQFG MAVSLGYYETL
Sbjct: 336 LEEHPQHELLKSMINKDYGYGGLLCLDMGTEERANRLMNLLQNYTQFGFMAVSLGYYETL 395

Query: 209 MXXXXXXXXXXXXXXEKKLAGISPGLIRMSIGYIGNLEQKWSQFEKAISRMQDS 48
           M              EK+LAGISPGL+RMSIGYIG LEQKWSQ EKA++RM DS
Sbjct: 396 MSCSGSSTSSEMNNEEKELAGISPGLVRMSIGYIGTLEQKWSQLEKALTRMHDS 449


>gb|EMJ10322.1| hypothetical protein PRUPE_ppa005470mg [Prunus persica]
          Length = 459

 Score =  167 bits (424), Expect = 1e-39
 Identities = 85/114 (74%), Positives = 91/114 (79%)
 Frame = -2

Query: 389 LEEHPQHQLLKSMANKDYGFSALVCLDMETEERANRLMNHLQNYTQFGLMAVSLGYYETL 210
           LE+HPQH+LLKS ANKDYGF  L+CLDM TEE+ANRLMN LQN TQFG MAVSLGYYETL
Sbjct: 340 LEDHPQHELLKSTANKDYGFGGLLCLDMGTEEKANRLMNLLQNCTQFGFMAVSLGYYETL 399

Query: 209 MXXXXXXXXXXXXXXEKKLAGISPGLIRMSIGYIGNLEQKWSQFEKAISRMQDS 48
           M              EK LAGISPGL+RMSIGYIG LEQ+WSQFEKAISRMQDS
Sbjct: 400 MSCSGSSTSSEMNAEEKALAGISPGLVRMSIGYIGTLEQRWSQFEKAISRMQDS 453


>ref|XP_003536171.1| PREDICTED: methionine gamma-lyase-like [Glycine max]
          Length = 442

 Score =  167 bits (424), Expect = 1e-39
 Identities = 81/116 (69%), Positives = 92/116 (79%)
 Frame = -2

Query: 389 LEEHPQHQLLKSMANKDYGFSALVCLDMETEERANRLMNHLQNYTQFGLMAVSLGYYETL 210
           LEEHPQHQLLKSM NKDYG+  L+C+DMETEERANR M+HLQNY QFG MAVSLGYYETL
Sbjct: 323 LEEHPQHQLLKSMHNKDYGYGGLMCVDMETEERANRFMSHLQNYGQFGFMAVSLGYYETL 382

Query: 209 MXXXXXXXXXXXXXXEKKLAGISPGLIRMSIGYIGNLEQKWSQFEKAISRMQDSSF 42
           M              EK+LAGISPGL+RMSIGYIG LEQKWSQ +KA++R+ DS +
Sbjct: 383 MSCSGSSTSSELSTEEKELAGISPGLVRMSIGYIGTLEQKWSQLQKALTRLNDSGY 438


>gb|ESR52463.1| hypothetical protein CICLE_v10020136mg [Citrus clementina]
          Length = 447

 Score =  166 bits (421), Expect = 2e-39
 Identities = 80/114 (70%), Positives = 92/114 (80%)
 Frame = -2

Query: 389 LEEHPQHQLLKSMANKDYGFSALVCLDMETEERANRLMNHLQNYTQFGLMAVSLGYYETL 210
           LE+HP H+LLKSMANK+YGF  L+CLDMETEE+ANRLMN+LQNY QFG MAVSLGYYETL
Sbjct: 329 LEDHPHHELLKSMANKEYGFGGLLCLDMETEEKANRLMNYLQNYAQFGFMAVSLGYYETL 388

Query: 209 MXXXXXXXXXXXXXXEKKLAGISPGLIRMSIGYIGNLEQKWSQFEKAISRMQDS 48
           M              EK LAGIS GL+RMS+GY+G L+Q+WSQFEKA+SRMQDS
Sbjct: 389 MSCSGSSTSSELNAEEKALAGISAGLVRMSVGYLGTLDQRWSQFEKALSRMQDS 442


>ref|XP_002299428.1| predicted protein [Populus trichocarpa] gi|222846686|gb|EEE84233.1|
           Cys/Met metabolism pyridoxal-phosphate-dependent enzyme
           family protein [Populus trichocarpa]
          Length = 454

 Score =  165 bits (418), Expect = 5e-39
 Identities = 79/114 (69%), Positives = 92/114 (80%)
 Frame = -2

Query: 389 LEEHPQHQLLKSMANKDYGFSALVCLDMETEERANRLMNHLQNYTQFGLMAVSLGYYETL 210
           LE+HPQH+LLKSMANK+YGF  L+C+DMETEE+ANRLMNHLQN TQFG MAVSLGYYETL
Sbjct: 336 LEDHPQHELLKSMANKEYGFGGLLCIDMETEEKANRLMNHLQNCTQFGFMAVSLGYYETL 395

Query: 209 MXXXXXXXXXXXXXXEKKLAGISPGLIRMSIGYIGNLEQKWSQFEKAISRMQDS 48
           M              E+ LAGISPGL+R+S+G++G L QKWSQFEKA SR+QDS
Sbjct: 396 MSCSGSSTSSELNDEEQALAGISPGLVRISVGFVGTLAQKWSQFEKAFSRLQDS 449


>gb|ABK96059.1| unknown [Populus trichocarpa]
          Length = 271

 Score =  165 bits (418), Expect = 5e-39
 Identities = 79/114 (69%), Positives = 92/114 (80%)
 Frame = -2

Query: 389 LEEHPQHQLLKSMANKDYGFSALVCLDMETEERANRLMNHLQNYTQFGLMAVSLGYYETL 210
           LE+HPQH+LLKSMANK+YGF  L+C+DMETEE+ANRLMNHLQN TQFG MAVSLGYYETL
Sbjct: 153 LEDHPQHELLKSMANKEYGFGGLLCIDMETEEKANRLMNHLQNCTQFGFMAVSLGYYETL 212

Query: 209 MXXXXXXXXXXXXXXEKKLAGISPGLIRMSIGYIGNLEQKWSQFEKAISRMQDS 48
           M              E+ LAGISPGL+R+S+G++G L QKWSQFEKA SR+QDS
Sbjct: 213 MSCSGSSTSSELNDEEQALAGISPGLVRISVGFVGTLAQKWSQFEKAFSRLQDS 266


>ref|XP_004495508.1| PREDICTED: methionine gamma-lyase-like [Cicer arietinum]
          Length = 441

 Score =  160 bits (405), Expect = 2e-37
 Identities = 77/116 (66%), Positives = 91/116 (78%)
 Frame = -2

Query: 389 LEEHPQHQLLKSMANKDYGFSALVCLDMETEERANRLMNHLQNYTQFGLMAVSLGYYETL 210
           LE+HP H+LLKSM NK+YG+  L+C+DMETEERAN+LMN+LQNY QFG MAVSLGYYETL
Sbjct: 322 LEDHPHHELLKSMHNKNYGYGGLLCIDMETEERANKLMNYLQNYGQFGFMAVSLGYYETL 381

Query: 209 MXXXXXXXXXXXXXXEKKLAGISPGLIRMSIGYIGNLEQKWSQFEKAISRMQDSSF 42
           M              EK LAGISPGL+RMSIGYIG LEQKW+Q EKA++R+Q+  F
Sbjct: 382 MSCSGNSTSSELTSEEKVLAGISPGLVRMSIGYIGTLEQKWTQLEKAVTRLQEYGF 437


>ref|XP_006343784.1| PREDICTED: methionine gamma-lyase-like [Solanum tuberosum]
          Length = 451

 Score =  160 bits (404), Expect = 2e-37
 Identities = 77/113 (68%), Positives = 91/113 (80%)
 Frame = -2

Query: 389 LEEHPQHQLLKSMANKDYGFSALVCLDMETEERANRLMNHLQNYTQFGLMAVSLGYYETL 210
           LE+HP H L+KSMANKDYG+  ++C+DMETEERANRLMN LQN+TQFG MAVSLGYYETL
Sbjct: 335 LEDHPDHALIKSMANKDYGYGGILCVDMETEERANRLMNVLQNFTQFGFMAVSLGYYETL 394

Query: 209 MXXXXXXXXXXXXXXEKKLAGISPGLIRMSIGYIGNLEQKWSQFEKAISRMQD 51
           M              EK+LAGISPGL+RMSIGY G+LEQKWSQ +KA+S+MQ+
Sbjct: 395 MSCSGSSTSSELNNEEKELAGISPGLVRMSIGYNGSLEQKWSQLDKALSKMQE 447


>gb|EPS64325.1| hypothetical protein M569_10456 [Genlisea aurea]
          Length = 180

 Score =  157 bits (396), Expect = 2e-36
 Identities = 75/115 (65%), Positives = 92/115 (80%)
 Frame = -2

Query: 389 LEEHPQHQLLKSMANKDYGFSALVCLDMETEERANRLMNHLQNYTQFGLMAVSLGYYETL 210
           L++HP H LLKS+ANKDYG+  L+C+DMETEE+A RLMN LQNY+QFGLMAVSLGYYETL
Sbjct: 61  LDDHPDHDLLKSLANKDYGYGGLLCVDMETEEKAYRLMNLLQNYSQFGLMAVSLGYYETL 120

Query: 209 MXXXXXXXXXXXXXXEKKLAGISPGLIRMSIGYIGNLEQKWSQFEKAISRMQDSS 45
           M              EK++AGISPGLIRMSIGY G+LEQKW+QFEKA++R+ + +
Sbjct: 121 MSCSGSSTSSEMNEEEKEMAGISPGLIRMSIGYSGSLEQKWNQFEKAVARLHEQN 175


>ref|XP_004245457.1| PREDICTED: methionine gamma-lyase-like [Solanum lycopersicum]
          Length = 444

 Score =  155 bits (392), Expect = 5e-36
 Identities = 75/111 (67%), Positives = 89/111 (80%)
 Frame = -2

Query: 389 LEEHPQHQLLKSMANKDYGFSALVCLDMETEERANRLMNHLQNYTQFGLMAVSLGYYETL 210
           LE+HP H L+KSMANK+YG+  ++C+DMETEERANRLMN LQN+TQFG MAVSLGYYETL
Sbjct: 332 LEDHPDHVLIKSMANKEYGYGGILCVDMETEERANRLMNVLQNFTQFGFMAVSLGYYETL 391

Query: 209 MXXXXXXXXXXXXXXEKKLAGISPGLIRMSIGYIGNLEQKWSQFEKAISRM 57
           M              EK+LAGISPGL+RMSIGY G+LEQKWSQ +KA+S+M
Sbjct: 392 MSCSGSSTSSELNNEEKELAGISPGLVRMSIGYNGSLEQKWSQLDKALSKM 442


>ref|XP_004244654.1| PREDICTED: methionine gamma-lyase-like [Solanum lycopersicum]
          Length = 409

 Score =  155 bits (392), Expect = 5e-36
 Identities = 77/112 (68%), Positives = 88/112 (78%)
 Frame = -2

Query: 389 LEEHPQHQLLKSMANKDYGFSALVCLDMETEERANRLMNHLQNYTQFGLMAVSLGYYETL 210
           LE HP H LLKS+ANKDYG+  ++C+DMETEE+AN LMN LQNYTQFGL+AVSLGYYETL
Sbjct: 298 LENHPDHVLLKSLANKDYGYGGILCVDMETEEKANCLMNVLQNYTQFGLIAVSLGYYETL 357

Query: 209 MXXXXXXXXXXXXXXEKKLAGISPGLIRMSIGYIGNLEQKWSQFEKAISRMQ 54
           M              EK+LAGISPGL+RMSIGY G LEQKWSQ EKA+S+MQ
Sbjct: 358 MSCSGNSTSSEMNNEEKELAGISPGLVRMSIGYNGTLEQKWSQLEKALSQMQ 409


>gb|AGF70153.1| methionine gamma-lyase [Cucumis melo]
          Length = 449

 Score =  155 bits (391), Expect = 6e-36
 Identities = 74/113 (65%), Positives = 91/113 (80%)
 Frame = -2

Query: 389 LEEHPQHQLLKSMANKDYGFSALVCLDMETEERANRLMNHLQNYTQFGLMAVSLGYYETL 210
           LE+HPQHQL+KS+AN +YGF  ++C+DM TEERAN+LM+ LQN TQFG MAVSLGYYETL
Sbjct: 329 LEDHPQHQLMKSLANPEYGFGGMLCVDMGTEERANKLMSILQNTTQFGFMAVSLGYYETL 388

Query: 209 MXXXXXXXXXXXXXXEKKLAGISPGLIRMSIGYIGNLEQKWSQFEKAISRMQD 51
           M              E++LAGISPGL+RMSIGY+G LEQKWSQFEKA++++QD
Sbjct: 389 MSCSGSSTSSEMSGEERELAGISPGLVRMSIGYMGTLEQKWSQFEKALAKVQD 441


>gb|ADN33936.1| cystathionine gamma-synthase [Cucumis melo subsp. melo]
          Length = 417

 Score =  155 bits (391), Expect = 6e-36
 Identities = 74/113 (65%), Positives = 91/113 (80%)
 Frame = -2

Query: 389 LEEHPQHQLLKSMANKDYGFSALVCLDMETEERANRLMNHLQNYTQFGLMAVSLGYYETL 210
           LE+HPQHQL+KS+AN +YGF  ++C+DM TEERAN+LM+ LQN TQFG MAVSLGYYETL
Sbjct: 297 LEDHPQHQLMKSLANPEYGFGGMLCVDMGTEERANKLMSILQNTTQFGFMAVSLGYYETL 356

Query: 209 MXXXXXXXXXXXXXXEKKLAGISPGLIRMSIGYIGNLEQKWSQFEKAISRMQD 51
           M              E++LAGISPGL+RMSIGY+G LEQKWSQFEKA++++QD
Sbjct: 357 MSCSGSSTSSEMSGEERELAGISPGLVRMSIGYMGTLEQKWSQFEKALAKVQD 409


>ref|XP_002280162.1| PREDICTED: methionine gamma-lyase [Vitis vinifera]
          Length = 438

 Score =  152 bits (385), Expect = 3e-35
 Identities = 77/112 (68%), Positives = 85/112 (75%)
 Frame = -2

Query: 389 LEEHPQHQLLKSMANKDYGFSALVCLDMETEERANRLMNHLQNYTQFGLMAVSLGYYETL 210
           LE HP H +LKS+AN DYGF  L+CLDM TEE+ANRLMN LQN TQFG MAVSLGYYETL
Sbjct: 326 LEGHPDHTVLKSIANLDYGFGGLLCLDMGTEEKANRLMNLLQNSTQFGFMAVSLGYYETL 385

Query: 209 MXXXXXXXXXXXXXXEKKLAGISPGLIRMSIGYIGNLEQKWSQFEKAISRMQ 54
           M              EK LAGISPGL+RMS+GY G LEQ+WSQFEKA+SRMQ
Sbjct: 386 MSCSGSSTSSEMNAEEKALAGISPGLVRMSVGYSGTLEQRWSQFEKALSRMQ 437


>gb|ABK27101.1| unknown [Picea sitchensis]
          Length = 438

 Score =  152 bits (383), Expect = 5e-35
 Identities = 74/113 (65%), Positives = 87/113 (76%)
 Frame = -2

Query: 389 LEEHPQHQLLKSMANKDYGFSALVCLDMETEERANRLMNHLQNYTQFGLMAVSLGYYETL 210
           LE+HPQ+ LLKSM N  YG+  ++C+DME+EERAN LM+HLQNYTQFGLMAVSLGYYETL
Sbjct: 319 LEDHPQYALLKSMVNSGYGYGGMLCVDMESEERANMLMSHLQNYTQFGLMAVSLGYYETL 378

Query: 209 MXXXXXXXXXXXXXXEKKLAGISPGLIRMSIGYIGNLEQKWSQFEKAISRMQD 51
           M              EK+LAGISPGLIRMSIGY G LEQ+WSQFE A++ M +
Sbjct: 379 MSCSGSSTSSEMNEEEKELAGISPGLIRMSIGYSGTLEQRWSQFENALTSMTE 431


>emb|CAN83499.1| hypothetical protein VITISV_026969 [Vitis vinifera]
          Length = 400

 Score =  150 bits (380), Expect = 1e-34
 Identities = 76/112 (67%), Positives = 84/112 (75%)
 Frame = -2

Query: 389 LEEHPQHQLLKSMANKDYGFSALVCLDMETEERANRLMNHLQNYTQFGLMAVSLGYYETL 210
           LE HP H +LKS+AN DYGF  L+CLDM TEE+ANRLMN LQN TQFG MAVSLGYYETL
Sbjct: 288 LEGHPDHTVLKSIANLDYGFGGLLCLDMGTEEKANRLMNLLQNSTQFGFMAVSLGYYETL 347

Query: 209 MXXXXXXXXXXXXXXEKKLAGISPGLIRMSIGYIGNLEQKWSQFEKAISRMQ 54
           M              EK LAGI PGL+RMS+GY G LEQ+WSQFEKA+SRMQ
Sbjct: 348 MSCSGSSTSSEMNAEEKALAGIXPGLVRMSVGYSGTLEQRWSQFEKALSRMQ 399


>ref|XP_006352296.1| PREDICTED: methionine gamma-lyase-like [Solanum tuberosum]
          Length = 433

 Score =  150 bits (379), Expect = 2e-34
 Identities = 75/112 (66%), Positives = 87/112 (77%)
 Frame = -2

Query: 389 LEEHPQHQLLKSMANKDYGFSALVCLDMETEERANRLMNHLQNYTQFGLMAVSLGYYETL 210
           LE HP H LLKS+AN+DYG+  ++C+DMETEE+AN LMN LQN TQFGL+AVSLGYYETL
Sbjct: 322 LENHPDHGLLKSLANEDYGYGGILCVDMETEEKANCLMNVLQNCTQFGLIAVSLGYYETL 381

Query: 209 MXXXXXXXXXXXXXXEKKLAGISPGLIRMSIGYIGNLEQKWSQFEKAISRMQ 54
           M              EK+LAGISPGL+RMSIGY G LEQKWSQ EKA+S+MQ
Sbjct: 382 MSCSGNSTSSEMNNQEKELAGISPGLVRMSIGYNGTLEQKWSQLEKALSQMQ 433