BLASTX nr result
ID: Glycyrrhiza36_contig00039912
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Glycyrrhiza36_contig00039912
(326 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ABN05767.1 FAD linked oxidase, N-terminal, partial [Medicago tru... 179 3e-52
XP_003595179.2 cytokinin oxidase/dehydrogenase-like protein [Med... 179 5e-52
ABN08744.1 FAD linked oxidase, N-terminal [Medicago truncatula] 179 6e-52
ALS05390.1 cytokinin oxidase/dehydrogenase 4 [Lotus japonicus] 178 2e-51
KYP72068.1 Cytokinin dehydrogenase 3 [Cajanus cajan] 160 4e-48
XP_004488073.1 PREDICTED: cytokinin dehydrogenase 2-like [Cicer ... 167 2e-47
KYP65760.1 Cytokinin dehydrogenase 3 [Cajanus cajan] 166 6e-47
XP_004488074.1 PREDICTED: cytokinin dehydrogenase 2-like [Cicer ... 166 7e-47
XP_007138721.1 hypothetical protein PHAVU_009G231800g [Phaseolus... 166 9e-47
XP_014506708.1 PREDICTED: cytokinin dehydrogenase 3-like [Vigna ... 163 1e-45
KYP46148.1 Cytokinin dehydrogenase 3 [Cajanus cajan] 159 4e-45
XP_014498992.1 PREDICTED: cytokinin dehydrogenase 2-like [Vigna ... 160 7e-45
XP_019462752.1 PREDICTED: cytokinin dehydrogenase 3-like [Lupinu... 160 7e-45
KYP46149.1 Cytokinin dehydrogenase 3 [Cajanus cajan] 159 2e-44
XP_017410347.1 PREDICTED: cytokinin dehydrogenase 4-like [Vigna ... 158 8e-44
OIV98266.1 hypothetical protein TanjilG_09900 [Lupinus angustifo... 154 1e-42
XP_019414495.1 PREDICTED: cytokinin dehydrogenase 2-like [Lupinu... 154 1e-42
XP_017420146.1 PREDICTED: cytokinin dehydrogenase 2-like [Vigna ... 154 2e-42
KYP65758.1 Cytokinin dehydrogenase 3 [Cajanus cajan] 154 2e-42
KYP46160.1 Cytokinin dehydrogenase 3 [Cajanus cajan] 153 6e-42
>ABN05767.1 FAD linked oxidase, N-terminal, partial [Medicago truncatula]
Length = 496
Score = 179 bits (455), Expect = 3e-52
Identities = 82/108 (75%), Positives = 94/108 (87%)
Frame = -2
Query: 325 FNEGVFKGIILKQNITAGIALIYPMNRNKWVDRMSAVTPNEDVFYTVGLLHAANGTDQVK 146
FNEGVFKGIILKQNI+AGI L+YPMNRNKW DRMSA+TP+EDVFYT+ LLHAA D+VK
Sbjct: 372 FNEGVFKGIILKQNISAGIVLVYPMNRNKWDDRMSAITPDEDVFYTLALLHAAKEMDEVK 431
Query: 145 TFLAQNQQILQFCKDAGIEIKEYLAGYKTQQEWMEHFGPKWQLIQDRK 2
TF AQN QILQFC DAGI++KEYL+G KT QEW+EHFG KWQ ++RK
Sbjct: 432 TFQAQNHQILQFCNDAGIKVKEYLSGNKTHQEWVEHFGAKWQQFEERK 479
>XP_003595179.2 cytokinin oxidase/dehydrogenase-like protein [Medicago truncatula]
AES65430.2 cytokinin oxidase/dehydrogenase-like protein
[Medicago truncatula]
Length = 522
Score = 179 bits (455), Expect = 5e-52
Identities = 82/108 (75%), Positives = 94/108 (87%)
Frame = -2
Query: 325 FNEGVFKGIILKQNITAGIALIYPMNRNKWVDRMSAVTPNEDVFYTVGLLHAANGTDQVK 146
FNEGVFKGIILKQNI+AGI L+YPMNRNKW DRMSA+TP+EDVFYT+ LLHAA D+VK
Sbjct: 398 FNEGVFKGIILKQNISAGIVLVYPMNRNKWDDRMSAITPDEDVFYTLALLHAAKEMDEVK 457
Query: 145 TFLAQNQQILQFCKDAGIEIKEYLAGYKTQQEWMEHFGPKWQLIQDRK 2
TF AQN QILQFC DAGI++KEYL+G KT QEW+EHFG KWQ ++RK
Sbjct: 458 TFQAQNHQILQFCNDAGIKVKEYLSGNKTHQEWVEHFGAKWQQFEERK 505
>ABN08744.1 FAD linked oxidase, N-terminal [Medicago truncatula]
Length = 535
Score = 179 bits (455), Expect = 6e-52
Identities = 82/108 (75%), Positives = 94/108 (87%)
Frame = -2
Query: 325 FNEGVFKGIILKQNITAGIALIYPMNRNKWVDRMSAVTPNEDVFYTVGLLHAANGTDQVK 146
FNEGVFKGIILKQNI+AGI L+YPMNRNKW DRMSA+TP+EDVFYT+ LLHAA D+VK
Sbjct: 411 FNEGVFKGIILKQNISAGIVLVYPMNRNKWDDRMSAITPDEDVFYTLALLHAAKEMDEVK 470
Query: 145 TFLAQNQQILQFCKDAGIEIKEYLAGYKTQQEWMEHFGPKWQLIQDRK 2
TF AQN QILQFC DAGI++KEYL+G KT QEW+EHFG KWQ ++RK
Sbjct: 471 TFQAQNHQILQFCNDAGIKVKEYLSGNKTHQEWVEHFGAKWQQFEERK 518
>ALS05390.1 cytokinin oxidase/dehydrogenase 4 [Lotus japonicus]
Length = 515
Score = 178 bits (451), Expect = 2e-51
Identities = 84/109 (77%), Positives = 96/109 (88%), Gaps = 1/109 (0%)
Frame = -2
Query: 325 FNEGVFKGIILKQNITAGIALIYPMNRNKWVDRMSAVTPNEDVFYTVGLLH-AANGTDQV 149
FNEGVFKGIILKQN+TAGI L+YPMNRNKW DRMS VTP+EDVFYTVGLL AANG D+V
Sbjct: 391 FNEGVFKGIILKQNLTAGIVLVYPMNRNKWDDRMSVVTPDEDVFYTVGLLQTAANGGDEV 450
Query: 148 KTFLAQNQQILQFCKDAGIEIKEYLAGYKTQQEWMEHFGPKWQLIQDRK 2
+ + AQNQ+ILQFCK AGI+IKEYL G KT +EW+EHFGPKW+L +DRK
Sbjct: 451 QKYQAQNQEILQFCKGAGIKIKEYLTGNKTHEEWVEHFGPKWKLWEDRK 499
>KYP72068.1 Cytokinin dehydrogenase 3 [Cajanus cajan]
Length = 171
Score = 160 bits (404), Expect = 4e-48
Identities = 73/108 (67%), Positives = 86/108 (79%)
Frame = -2
Query: 325 FNEGVFKGIILKQNITAGIALIYPMNRNKWVDRMSAVTPNEDVFYTVGLLHAANGTDQVK 146
FNEGVFK IILKQNI G L+YPMNRNKW DRMSAV P EDVFY+V + +A+G D+V+
Sbjct: 47 FNEGVFKDIILKQNIPPGACLVYPMNRNKWDDRMSAVIPEEDVFYSVNIFQSASGLDEVE 106
Query: 145 TFLAQNQQILQFCKDAGIEIKEYLAGYKTQQEWMEHFGPKWQLIQDRK 2
+ QNQ+ILQFCKD I+IKEYL G KT QEW+EHFG KW+L +DRK
Sbjct: 107 KYQVQNQKILQFCKDTNIKIKEYLTGNKTHQEWVEHFGLKWKLFEDRK 154
>XP_004488073.1 PREDICTED: cytokinin dehydrogenase 2-like [Cicer arietinum]
Length = 526
Score = 167 bits (424), Expect = 2e-47
Identities = 77/108 (71%), Positives = 91/108 (84%)
Frame = -2
Query: 325 FNEGVFKGIILKQNITAGIALIYPMNRNKWVDRMSAVTPNEDVFYTVGLLHAANGTDQVK 146
FNEGVFKGIILKQN+T I L YPMN+NKW D+MSAV PNEDVFYT+ LL+AA+G D+VK
Sbjct: 402 FNEGVFKGIILKQNLTPAILLFYPMNQNKWDDKMSAVIPNEDVFYTLSLLNAASGLDEVK 461
Query: 145 TFLAQNQQILQFCKDAGIEIKEYLAGYKTQQEWMEHFGPKWQLIQDRK 2
F AQ QQILQFC D+GIEI++YL G KT Q+W++HFG KWQL +DRK
Sbjct: 462 AFEAQIQQILQFCNDSGIEIRQYLIGNKTPQQWIQHFGTKWQLFEDRK 509
>KYP65760.1 Cytokinin dehydrogenase 3 [Cajanus cajan]
Length = 518
Score = 166 bits (420), Expect = 6e-47
Identities = 80/110 (72%), Positives = 91/110 (82%), Gaps = 2/110 (1%)
Frame = -2
Query: 325 FNEGVFKGIILKQNITAGIALIYPMNRNKWVDRMSAVTPNEDVFYTVGLLHAANG--TDQ 152
FNEGVFK IILKQNI++GI+LIYPMNRNKW DRMSAVTP EDVFY + LLH A G D
Sbjct: 392 FNEGVFKDIILKQNISSGISLIYPMNRNKWDDRMSAVTPAEDVFYAISLLHTAIGLDIDG 451
Query: 151 VKTFLAQNQQILQFCKDAGIEIKEYLAGYKTQQEWMEHFGPKWQLIQDRK 2
V+ F AQNQQILQFCK+A I+I EYL G KT QEW+EHFGPKW++ +DRK
Sbjct: 452 VQKFQAQNQQILQFCKNASIKITEYLTGNKTHQEWVEHFGPKWKVFEDRK 501
>XP_004488074.1 PREDICTED: cytokinin dehydrogenase 2-like [Cicer arietinum]
Length = 526
Score = 166 bits (420), Expect = 7e-47
Identities = 78/108 (72%), Positives = 90/108 (83%)
Frame = -2
Query: 325 FNEGVFKGIILKQNITAGIALIYPMNRNKWVDRMSAVTPNEDVFYTVGLLHAANGTDQVK 146
FNEGVFK IILKQN+T I L YPMNRNKW D+MSAV PNEDVFYT+GLL+AA+G D+VK
Sbjct: 402 FNEGVFKDIILKQNLTPVILLFYPMNRNKWDDKMSAVIPNEDVFYTLGLLNAASGLDEVK 461
Query: 145 TFLAQNQQILQFCKDAGIEIKEYLAGYKTQQEWMEHFGPKWQLIQDRK 2
F AQ QQILQFC D+GIEIK+YL KT Q+W++HFG KWQL +DRK
Sbjct: 462 AFEAQIQQILQFCNDSGIEIKQYLTRNKTPQQWIQHFGTKWQLFEDRK 509
>XP_007138721.1 hypothetical protein PHAVU_009G231800g [Phaseolus vulgaris]
ESW10715.1 hypothetical protein PHAVU_009G231800g
[Phaseolus vulgaris]
Length = 516
Score = 166 bits (419), Expect = 9e-47
Identities = 78/108 (72%), Positives = 86/108 (79%)
Frame = -2
Query: 325 FNEGVFKGIILKQNITAGIALIYPMNRNKWVDRMSAVTPNEDVFYTVGLLHAANGTDQVK 146
FNEGVFK IIL QN+++GIALIYPMNRNKW D+MS VTP EDVFY V LL A + D VK
Sbjct: 384 FNEGVFKNIILAQNLSSGIALIYPMNRNKWDDKMSPVTPEEDVFYAVSLLRATSSRDMVK 443
Query: 145 TFLAQNQQILQFCKDAGIEIKEYLAGYKTQQEWMEHFGPKWQLIQDRK 2
QNQQILQFCKDAGI+I EYL G KTQQ+WMEHFG KW+L DRK
Sbjct: 444 KLQDQNQQILQFCKDAGIQITEYLTGNKTQQQWMEHFGSKWELFADRK 491
>XP_014506708.1 PREDICTED: cytokinin dehydrogenase 3-like [Vigna radiata var.
radiata]
Length = 522
Score = 163 bits (412), Expect = 1e-45
Identities = 77/108 (71%), Positives = 93/108 (86%)
Frame = -2
Query: 325 FNEGVFKGIILKQNITAGIALIYPMNRNKWVDRMSAVTPNEDVFYTVGLLHAANGTDQVK 146
F+EGVFKGIILKQNITAG+A+IYPMNR+KW D+MSAVTPNEDVFYTV LLH + G D+V+
Sbjct: 397 FDEGVFKGIILKQNITAGVAIIYPMNRSKWNDQMSAVTPNEDVFYTVSLLH-STGFDKVE 455
Query: 145 TFLAQNQQILQFCKDAGIEIKEYLAGYKTQQEWMEHFGPKWQLIQDRK 2
F AQNQQIL+FCKDAGIEIK+YL KT+ EW++ FG KW+ ++RK
Sbjct: 456 EFQAQNQQILKFCKDAGIEIKQYLPQNKTRNEWIKQFGSKWKTFEERK 503
>KYP46148.1 Cytokinin dehydrogenase 3 [Cajanus cajan]
Length = 405
Score = 159 bits (402), Expect = 4e-45
Identities = 73/108 (67%), Positives = 88/108 (81%)
Frame = -2
Query: 325 FNEGVFKGIILKQNITAGIALIYPMNRNKWVDRMSAVTPNEDVFYTVGLLHAANGTDQVK 146
FNEGVFKGIILKQNI+ G L+YPM RNKW RMSAV P+EDVFY+V L H + + VK
Sbjct: 281 FNEGVFKGIILKQNISPGTCLVYPMKRNKWDGRMSAVIPDEDVFYSVNLFHYVSASYNVK 340
Query: 145 TFLAQNQQILQFCKDAGIEIKEYLAGYKTQQEWMEHFGPKWQLIQDRK 2
+ AQNQ+IL+FCKDAGI+IKEYLAG KT Q+W+EHFG KW+L ++RK
Sbjct: 341 DYEAQNQKILEFCKDAGIKIKEYLAGNKTHQQWVEHFGSKWKLFEERK 388
>XP_014498992.1 PREDICTED: cytokinin dehydrogenase 2-like [Vigna radiata var.
radiata]
Length = 497
Score = 160 bits (405), Expect = 7e-45
Identities = 72/108 (66%), Positives = 89/108 (82%)
Frame = -2
Query: 325 FNEGVFKGIILKQNITAGIALIYPMNRNKWVDRMSAVTPNEDVFYTVGLLHAANGTDQVK 146
FNEGVFK IILKQNI++GI+LIYP+NRNKW D+MS VTP+EDVFY + LL + + D ++
Sbjct: 372 FNEGVFKNIILKQNISSGISLIYPLNRNKWDDKMSPVTPDEDVFYALSLLRSTSSMDMLE 431
Query: 145 TFLAQNQQILQFCKDAGIEIKEYLAGYKTQQEWMEHFGPKWQLIQDRK 2
+ QN+QIL+FCKDAGI+I EYL G KTQQ+WMEHFG KW+L DRK
Sbjct: 432 KYQTQNRQILEFCKDAGIKITEYLTGNKTQQQWMEHFGSKWKLFADRK 479
>XP_019462752.1 PREDICTED: cytokinin dehydrogenase 3-like [Lupinus angustifolius]
Length = 522
Score = 160 bits (406), Expect = 7e-45
Identities = 73/108 (67%), Positives = 89/108 (82%)
Frame = -2
Query: 325 FNEGVFKGIILKQNITAGIALIYPMNRNKWVDRMSAVTPNEDVFYTVGLLHAANGTDQVK 146
F+EGVFKGIILKQNITAG+ L+YPMNR+KW D+MSAVTPNEDVFY V LLH A DQ++
Sbjct: 398 FDEGVFKGIILKQNITAGVVLVYPMNRSKWNDKMSAVTPNEDVFYVVSLLHTAATLDQLE 457
Query: 145 TFLAQNQQILQFCKDAGIEIKEYLAGYKTQQEWMEHFGPKWQLIQDRK 2
F AQNQQILQFC+DA I +K+YL KT+++W+E FGPKW+ + RK
Sbjct: 458 IFKAQNQQILQFCEDASIGVKQYLPQNKTREQWVEQFGPKWKSFEQRK 505
>KYP46149.1 Cytokinin dehydrogenase 3 [Cajanus cajan]
Length = 489
Score = 159 bits (402), Expect = 2e-44
Identities = 73/108 (67%), Positives = 88/108 (81%)
Frame = -2
Query: 325 FNEGVFKGIILKQNITAGIALIYPMNRNKWVDRMSAVTPNEDVFYTVGLLHAANGTDQVK 146
FNEGVFKGIILKQNI+ G L+YPM RNKW RMSAV P+EDVFY+V L H + + VK
Sbjct: 365 FNEGVFKGIILKQNISPGTCLVYPMKRNKWDGRMSAVIPDEDVFYSVNLFHYVSASYNVK 424
Query: 145 TFLAQNQQILQFCKDAGIEIKEYLAGYKTQQEWMEHFGPKWQLIQDRK 2
+ AQNQ+IL+FCKDAGI+IKEYLAG KT Q+W+EHFG KW+L ++RK
Sbjct: 425 DYEAQNQKILEFCKDAGIKIKEYLAGNKTHQQWVEHFGSKWKLFEERK 472
>XP_017410347.1 PREDICTED: cytokinin dehydrogenase 4-like [Vigna angularis]
BAT76819.1 hypothetical protein VIGAN_01487700 [Vigna
angularis var. angularis]
Length = 520
Score = 158 bits (399), Expect = 8e-44
Identities = 75/108 (69%), Positives = 92/108 (85%)
Frame = -2
Query: 325 FNEGVFKGIILKQNITAGIALIYPMNRNKWVDRMSAVTPNEDVFYTVGLLHAANGTDQVK 146
F+EGVFKGIILKQNITAG+A+IYPMNR+KW D+MSAVTPNEDVFYTV LLH + G D+V+
Sbjct: 397 FDEGVFKGIILKQNITAGVAIIYPMNRSKWNDKMSAVTPNEDVFYTVSLLH-STGFDKVE 455
Query: 145 TFLAQNQQILQFCKDAGIEIKEYLAGYKTQQEWMEHFGPKWQLIQDRK 2
F AQNQ+IL+FC+DAGI+IK+YL KT+ EW++ FG KW+ Q RK
Sbjct: 456 EFQAQNQRILKFCEDAGIKIKQYLPQNKTRNEWIKQFGSKWKTFQVRK 503
>OIV98266.1 hypothetical protein TanjilG_09900 [Lupinus angustifolius]
Length = 494
Score = 154 bits (390), Expect = 1e-42
Identities = 76/110 (69%), Positives = 87/110 (79%), Gaps = 2/110 (1%)
Frame = -2
Query: 325 FNEGVFKGIILKQNITAGIALIYPMNRNKWVDRMSAVTPNEDVFYTVGLLHAANGTD--Q 152
FNEGV KGIILKQNI+ G YP NRNKW RMSAVTPNED+FY +GL + ++G D +
Sbjct: 369 FNEGVLKGIILKQNISVGTPSFYPTNRNKWDYRMSAVTPNEDIFYVLGL-YRSSGFDKVE 427
Query: 151 VKTFLAQNQQILQFCKDAGIEIKEYLAGYKTQQEWMEHFGPKWQLIQDRK 2
V+ AQNQQILQFCKD GIEIKEYLA YKT +EWME +G KWQLI+DRK
Sbjct: 428 VEAHEAQNQQILQFCKDVGIEIKEYLASYKTHEEWMEQYGSKWQLIEDRK 477
>XP_019414495.1 PREDICTED: cytokinin dehydrogenase 2-like [Lupinus angustifolius]
Length = 518
Score = 154 bits (390), Expect = 1e-42
Identities = 76/110 (69%), Positives = 87/110 (79%), Gaps = 2/110 (1%)
Frame = -2
Query: 325 FNEGVFKGIILKQNITAGIALIYPMNRNKWVDRMSAVTPNEDVFYTVGLLHAANGTD--Q 152
FNEGV KGIILKQNI+ G YP NRNKW RMSAVTPNED+FY +GL + ++G D +
Sbjct: 393 FNEGVLKGIILKQNISVGTPSFYPTNRNKWDYRMSAVTPNEDIFYVLGL-YRSSGFDKVE 451
Query: 151 VKTFLAQNQQILQFCKDAGIEIKEYLAGYKTQQEWMEHFGPKWQLIQDRK 2
V+ AQNQQILQFCKD GIEIKEYLA YKT +EWME +G KWQLI+DRK
Sbjct: 452 VEAHEAQNQQILQFCKDVGIEIKEYLASYKTHEEWMEQYGSKWQLIEDRK 501
>XP_017420146.1 PREDICTED: cytokinin dehydrogenase 2-like [Vigna angularis]
XP_017420147.1 PREDICTED: cytokinin dehydrogenase 2-like
[Vigna angularis]
Length = 500
Score = 154 bits (389), Expect = 2e-42
Identities = 70/107 (65%), Positives = 86/107 (80%)
Frame = -2
Query: 322 NEGVFKGIILKQNITAGIALIYPMNRNKWVDRMSAVTPNEDVFYTVGLLHAANGTDQVKT 143
NEGVFK IILKQNI+ GI+LIYP+NR+KW D+MS VTP+EDVFY V LL + + D +K
Sbjct: 378 NEGVFKNIILKQNISGGISLIYPLNRSKWDDKMSPVTPDEDVFYAVSLLRSTSSIDMLKK 437
Query: 142 FLAQNQQILQFCKDAGIEIKEYLAGYKTQQEWMEHFGPKWQLIQDRK 2
+ QNQQIL+FCK AGI+I +YL G KTQQ+W+EHFG KW+L DRK
Sbjct: 438 YQTQNQQILEFCKGAGIKITKYLTGNKTQQQWVEHFGSKWKLFADRK 484
>KYP65758.1 Cytokinin dehydrogenase 3 [Cajanus cajan]
Length = 527
Score = 154 bits (390), Expect = 2e-42
Identities = 73/111 (65%), Positives = 88/111 (79%), Gaps = 3/111 (2%)
Frame = -2
Query: 325 FNEGVFKGIILKQNIT--AGIALIYPMNRNKWVDRMSAVTPNEDVFYTVGLLH-AANGTD 155
FNEGVF+GI+++QN+T AG+ L+YPMNRNKW DRMSAVTP+ED+FY+V + A +G
Sbjct: 400 FNEGVFRGIVVEQNVTSQAGLYLVYPMNRNKWDDRMSAVTPDEDIFYSVNFFNFATSGFK 459
Query: 154 QVKTFLAQNQQILQFCKDAGIEIKEYLAGYKTQQEWMEHFGPKWQLIQDRK 2
K F QNQ LQFCKDAGIEIKEYL KT QEW+EHFGPKW+L +DRK
Sbjct: 460 LAKKFQVQNQLTLQFCKDAGIEIKEYLPSKKTHQEWVEHFGPKWKLFEDRK 510
>KYP46160.1 Cytokinin dehydrogenase 3 [Cajanus cajan]
Length = 526
Score = 153 bits (386), Expect = 6e-42
Identities = 75/108 (69%), Positives = 85/108 (78%)
Frame = -2
Query: 325 FNEGVFKGIILKQNITAGIALIYPMNRNKWVDRMSAVTPNEDVFYTVGLLHAANGTDQVK 146
FNEGVFK IILKQNI+ G L+YPMNRNKW DRMSAVTP EDVFY+V L A V+
Sbjct: 404 FNEGVFKDIILKQNISPGGCLVYPMNRNKWDDRMSAVTPKEDVFYSVNLFRFA--VQNVE 461
Query: 145 TFLAQNQQILQFCKDAGIEIKEYLAGYKTQQEWMEHFGPKWQLIQDRK 2
+ QNQ+ILQFCKDA I+IKEYLAG KT QEW+EHFG KW+L +DRK
Sbjct: 462 KYEVQNQKILQFCKDADIKIKEYLAGNKTHQEWVEHFGLKWKLFKDRK 509