BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Glycyrrhiza36_contig00010524
         (1265 letters)

Database: ./nr 
           115,041,592 sequences; 42,171,959,267 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_003537603.1 PREDICTED: lipid phosphate phosphatase epsilon 2,...   411   e-140
XP_006590698.1 PREDICTED: lipid phosphate phosphatase epsilon 1,...   406   e-138
KRH76744.1 hypothetical protein GLYMA_01G171800 [Glycine max]         402   e-136
KHN36083.1 Dolichyldiphosphatase [Glycine soja]                       401   e-136
XP_017426791.1 PREDICTED: lipid phosphate phosphatase epsilon 2,...   399   e-135
NP_001242862.1 uncharacterized protein LOC100791542 [Glycine max...   395   e-134
XP_014519971.1 PREDICTED: lipid phosphate phosphatase epsilon 2,...   395   e-134
XP_007156981.1 hypothetical protein PHAVU_002G033600g [Phaseolus...   386   e-130
KYP66800.1 hypothetical protein KK1_013111 [Cajanus cajan]            381   e-129
AFK49290.1 unknown [Lotus japonicus]                                  372   e-125
XP_019444786.1 PREDICTED: lipid phosphate phosphatase epsilon 1,...   347   e-115
XP_013446388.1 phosphatidic acid phosphatase (PAP2) family prote...   335   e-110
XP_004509286.1 PREDICTED: lipid phosphate phosphatase epsilon 1,...   331   e-109
XP_016201895.1 PREDICTED: lipid phosphate phosphatase epsilon 1,...   315   e-102
XP_015964140.1 PREDICTED: lipid phosphate phosphatase epsilon 1,...   315   e-102
GAU36018.1 hypothetical protein TSUD_211560 [Trifolium subterran...   308   e-99 
AFK34692.1 unknown [Lotus japonicus]                                  297   8e-96
XP_002263902.1 PREDICTED: lipid phosphate phosphatase epsilon 1,...   292   9e-94
XP_002521546.1 PREDICTED: lipid phosphate phosphatase epsilon 2,...   289   2e-92
XP_018829479.1 PREDICTED: lipid phosphate phosphatase epsilon 2,...   288   7e-92

>XP_003537603.1 PREDICTED: lipid phosphate phosphatase epsilon 2, chloroplastic
            isoform X2 [Glycine max] KHN41947.1 Dolichyldiphosphatase
            1 [Glycine soja] KRH28717.1 hypothetical protein
            GLYMA_11G071400 [Glycine max]
          Length = 287

 Score =  411 bits (1056), Expect = e-140
 Identities = 208/280 (74%), Positives = 229/280 (81%), Gaps = 1/280 (0%)
 Frame = -3

Query: 1026 CYTPS-YLVQRPNLLRQRHLKNTXXXXXXXXXXXFLCGGFVRRKLVFERYTLWVPNTMNE 850
            CY PS Y++   NLLRQ HLKNT           FLC GFV RK V  R + WV  TM+E
Sbjct: 8    CYGPSSYILLGSNLLRQGHLKNTSFAHRFSPSRSFLCNGFVPRKPVLGRNSFWVSKTMDE 67

Query: 849  STRISAFRDGEGDENIQVFEQEALIDGSSVSRPMFLSREMEDTLNRLSKWIVAAFFGGFI 670
            S R SAFRDG+GDE IQVFEQEA IDGS+  +  FLS E+E  LNR+SKWIV A FGGFI
Sbjct: 68   SARTSAFRDGKGDETIQVFEQEAFIDGSTPFQSKFLSPEVEYNLNRMSKWIVTALFGGFI 127

Query: 669  LWRHDAEALWFTAGSILNALLSLWLKRILNQERPSTLKSDPGMPSSHAQSLFFSAVFVIL 490
            LWRHDAEALWFTAGS+LNA+LS+ LKRILNQERPSTLKSDPGMPSSHAQS+FF+  FVIL
Sbjct: 128  LWRHDAEALWFTAGSVLNAMLSVLLKRILNQERPSTLKSDPGMPSSHAQSIFFTVFFVIL 187

Query: 489  SSVELLGLNGFTIAISGLALAFGSFFSYLRVSQQLHTISQVVVGAAIGSVFSILWYWLWN 310
            S VE LGLNGFTIAIS   L FGSFFSYLRVSQQLHT+SQVVVGAAIGS+FSILWYWLWN
Sbjct: 188  SGVEWLGLNGFTIAISASVLTFGSFFSYLRVSQQLHTVSQVVVGAAIGSIFSILWYWLWN 247

Query: 309  AIVLDAFASSLWVRIIVVLGSAGLCLGFVVFAIRHWLRDD 190
              +LDAF SSLWVRIIVVLGSAGLC+GFV+FAIR+WL+DD
Sbjct: 248  GYMLDAFVSSLWVRIIVVLGSAGLCIGFVLFAIRYWLQDD 287


>XP_006590698.1 PREDICTED: lipid phosphate phosphatase epsilon 1, chloroplastic
            isoform X1 [Glycine max] KRH28718.1 hypothetical protein
            GLYMA_11G071400 [Glycine max]
          Length = 289

 Score =  406 bits (1043), Expect = e-138
 Identities = 208/282 (73%), Positives = 229/282 (81%), Gaps = 3/282 (1%)
 Frame = -3

Query: 1026 CYTPS-YLVQRPNLLRQRHLKNTXXXXXXXXXXXFLCGGFVRRKLVFERYTLWVPNTMNE 850
            CY PS Y++   NLLRQ HLKNT           FLC GFV RK V  R + WV  TM+E
Sbjct: 8    CYGPSSYILLGSNLLRQGHLKNTSFAHRFSPSRSFLCNGFVPRKPVLGRNSFWVSKTMDE 67

Query: 849  STRISAFRDGEGDENIQVFEQEALIDGSSVSRPMFLSREMEDTLNRLSKWIVAAFFGGFI 670
            S R SAFRDG+GDE IQVFEQEA IDGS+  +  FLS E+E  LNR+SKWIV A FGGFI
Sbjct: 68   SARTSAFRDGKGDETIQVFEQEAFIDGSTPFQSKFLSPEVEYNLNRMSKWIVTALFGGFI 127

Query: 669  LWRHDAEALWFTAGSILNALLSLWLKRILNQERPSTLKSDPGMPSSHAQSLFFSAVFVIL 490
            LWRHDAEALWFTAGS+LNA+LS+ LKRILNQERPSTLKSDPGMPSSHAQS+FF+  FVIL
Sbjct: 128  LWRHDAEALWFTAGSVLNAMLSVLLKRILNQERPSTLKSDPGMPSSHAQSIFFTVFFVIL 187

Query: 489  SSVELLGLNGFTIAISGLALAFGSFF--SYLRVSQQLHTISQVVVGAAIGSVFSILWYWL 316
            S VE LGLNGFTIAIS   L FGSFF  SYLRVSQQLHT+SQVVVGAAIGS+FSILWYWL
Sbjct: 188  SGVEWLGLNGFTIAISASVLTFGSFFVSSYLRVSQQLHTVSQVVVGAAIGSIFSILWYWL 247

Query: 315  WNAIVLDAFASSLWVRIIVVLGSAGLCLGFVVFAIRHWLRDD 190
            WN  +LDAF SSLWVRIIVVLGSAGLC+GFV+FAIR+WL+DD
Sbjct: 248  WNGYMLDAFVSSLWVRIIVVLGSAGLCIGFVLFAIRYWLQDD 289


>KRH76744.1 hypothetical protein GLYMA_01G171800 [Glycine max]
          Length = 286

 Score =  402 bits (1032), Expect = e-136
 Identities = 203/279 (72%), Positives = 221/279 (79%)
 Frame = -3

Query: 1026 CYTPSYLVQRPNLLRQRHLKNTXXXXXXXXXXXFLCGGFVRRKLVFERYTLWVPNTMNES 847
            CY PS +V   NLL++RHLKNT            LC GFV RK    R + WV   M+ES
Sbjct: 8    CYGPSSIVLGSNLLKRRHLKNTSFADRFSPSRSLLCSGFVPRKPALGRNSFWVSKIMDES 67

Query: 846  TRISAFRDGEGDENIQVFEQEALIDGSSVSRPMFLSREMEDTLNRLSKWIVAAFFGGFIL 667
               SAFRD +GDE IQVFEQEA IDGSS  R  FLS E+E  LNRLSKWIV A FG FIL
Sbjct: 68   VGTSAFRDAKGDETIQVFEQEAFIDGSSPFRFKFLSPEVEYKLNRLSKWIVTALFGSFIL 127

Query: 666  WRHDAEALWFTAGSILNALLSLWLKRILNQERPSTLKSDPGMPSSHAQSLFFSAVFVILS 487
            WRHDAEALWFTAGS+LNA+LS+ LKRILNQERPSTLKSDPGMPSSHAQS+FF+  FVILS
Sbjct: 128  WRHDAEALWFTAGSVLNAMLSVLLKRILNQERPSTLKSDPGMPSSHAQSIFFTVFFVILS 187

Query: 486  SVELLGLNGFTIAISGLALAFGSFFSYLRVSQQLHTISQVVVGAAIGSVFSILWYWLWNA 307
             VE LGLNGFTIAISGL L FGSF SYLRV QQLHT+SQVVVGAAIGS+ SILWYWLWN 
Sbjct: 188  GVEWLGLNGFTIAISGLVLTFGSFLSYLRVVQQLHTVSQVVVGAAIGSISSILWYWLWNG 247

Query: 306  IVLDAFASSLWVRIIVVLGSAGLCLGFVVFAIRHWLRDD 190
             +LDAF SSLWVRIIVVLGSAGLC+GFV+F IRHWL+DD
Sbjct: 248  YMLDAFVSSLWVRIIVVLGSAGLCIGFVLFVIRHWLQDD 286


>KHN36083.1 Dolichyldiphosphatase [Glycine soja]
          Length = 286

 Score =  401 bits (1030), Expect = e-136
 Identities = 202/279 (72%), Positives = 221/279 (79%)
 Frame = -3

Query: 1026 CYTPSYLVQRPNLLRQRHLKNTXXXXXXXXXXXFLCGGFVRRKLVFERYTLWVPNTMNES 847
            CY PS +V   NLL++RHLKNT            LC GFV RK    R + WV   M+ES
Sbjct: 8    CYGPSSIVLGSNLLKRRHLKNTSFADRFSPSRSLLCSGFVPRKPALGRNSFWVSKIMDES 67

Query: 846  TRISAFRDGEGDENIQVFEQEALIDGSSVSRPMFLSREMEDTLNRLSKWIVAAFFGGFIL 667
               SAFRD +GDE IQVFEQEA IDGSS  R  FLS E+E  LNRLSKWIV A FG FIL
Sbjct: 68   VGTSAFRDAKGDETIQVFEQEAFIDGSSPFRFKFLSPEVEYKLNRLSKWIVTALFGSFIL 127

Query: 666  WRHDAEALWFTAGSILNALLSLWLKRILNQERPSTLKSDPGMPSSHAQSLFFSAVFVILS 487
            WRHDAEALWFTAGS+LNA+LS+ LKRILNQERPSTLKSDPGMPSSHAQS+FF+  FVILS
Sbjct: 128  WRHDAEALWFTAGSVLNAMLSVLLKRILNQERPSTLKSDPGMPSSHAQSIFFTVFFVILS 187

Query: 486  SVELLGLNGFTIAISGLALAFGSFFSYLRVSQQLHTISQVVVGAAIGSVFSILWYWLWNA 307
             VE LGLNGFTIAISGL L FGSF SYLRV QQLHT+SQVVVGAAIGS+ SILWYWLWN 
Sbjct: 188  GVEWLGLNGFTIAISGLVLTFGSFLSYLRVVQQLHTVSQVVVGAAIGSISSILWYWLWNG 247

Query: 306  IVLDAFASSLWVRIIVVLGSAGLCLGFVVFAIRHWLRDD 190
             ++DAF SSLWVRIIVVLGSAGLC+GFV+F IRHWL+DD
Sbjct: 248  YMMDAFVSSLWVRIIVVLGSAGLCIGFVLFVIRHWLQDD 286


>XP_017426791.1 PREDICTED: lipid phosphate phosphatase epsilon 2, chloroplastic
            [Vigna angularis] BAU00043.1 hypothetical protein
            VIGAN_10160000 [Vigna angularis var. angularis]
          Length = 286

 Score =  399 bits (1025), Expect = e-135
 Identities = 197/279 (70%), Positives = 221/279 (79%)
 Frame = -3

Query: 1026 CYTPSYLVQRPNLLRQRHLKNTXXXXXXXXXXXFLCGGFVRRKLVFERYTLWVPNTMNES 847
            C  P  L Q  N L++R LKNT            LC GF+ R+ +  R + WV  TM+ES
Sbjct: 8    CQAPFTLFQGSNCLKRRKLKNTSLPRCFSPSRSLLCSGFLSRRPLLRRNSFWVSKTMDES 67

Query: 846  TRISAFRDGEGDENIQVFEQEALIDGSSVSRPMFLSREMEDTLNRLSKWIVAAFFGGFIL 667
             R SAFRDGEGDENIQVFEQEA IDG S  +  FLS E+E  LN+LSKWIV A FGGFIL
Sbjct: 68   VRTSAFRDGEGDENIQVFEQEAFIDGPSPFQSKFLSHEVEYKLNKLSKWIVTALFGGFIL 127

Query: 666  WRHDAEALWFTAGSILNALLSLWLKRILNQERPSTLKSDPGMPSSHAQSLFFSAVFVILS 487
            WRHDAEALWFT+GSILNALLS+ LKRILNQERPSTLKSDPGMPSSHAQS+FF+  FVILS
Sbjct: 128  WRHDAEALWFTSGSILNALLSVLLKRILNQERPSTLKSDPGMPSSHAQSIFFAVFFVILS 187

Query: 486  SVELLGLNGFTIAISGLALAFGSFFSYLRVSQQLHTISQVVVGAAIGSVFSILWYWLWNA 307
             VE LGLN FTIA+SGL L FGSFFSYLRVSQQLHT++QVVVGA IGS+FSILWYWLWN 
Sbjct: 188  GVEWLGLNVFTIAMSGLVLTFGSFFSYLRVSQQLHTVNQVVVGAVIGSIFSILWYWLWNG 247

Query: 306  IVLDAFASSLWVRIIVVLGSAGLCLGFVVFAIRHWLRDD 190
             +LD F SSLWVRI+VVLGS GLC+GFV+F IR+WL+DD
Sbjct: 248  FILDVFVSSLWVRIVVVLGSVGLCIGFVLFTIRYWLQDD 286


>NP_001242862.1 uncharacterized protein LOC100791542 [Glycine max] ACU17907.1 unknown
            [Glycine max]
          Length = 286

 Score =  395 bits (1016), Expect = e-134
 Identities = 202/279 (72%), Positives = 220/279 (78%)
 Frame = -3

Query: 1026 CYTPSYLVQRPNLLRQRHLKNTXXXXXXXXXXXFLCGGFVRRKLVFERYTLWVPNTMNES 847
            CY PS +V   NLL++RHLKNT            LC GFV RK    R + WV   M+ES
Sbjct: 8    CYGPSSIVLGSNLLKRRHLKNTSFADRFSPSRSLLCSGFVPRKPALGRNSFWVSKIMDES 67

Query: 846  TRISAFRDGEGDENIQVFEQEALIDGSSVSRPMFLSREMEDTLNRLSKWIVAAFFGGFIL 667
               SAFRD +GDE IQVFEQEA IDGSS  R  FLS E+E  LNRLSKWIV A FG FIL
Sbjct: 68   VGTSAFRDTKGDETIQVFEQEAFIDGSSPFRFKFLSPEVEYKLNRLSKWIVTALFGSFIL 127

Query: 666  WRHDAEALWFTAGSILNALLSLWLKRILNQERPSTLKSDPGMPSSHAQSLFFSAVFVILS 487
            WRHDAEALWFTAGS+LNA+LS+ LKRILNQERPSTLKSDPGMPSSHAQS+FF+  FVILS
Sbjct: 128  WRHDAEALWFTAGSVLNAMLSVLLKRILNQERPSTLKSDPGMPSSHAQSIFFTVFFVILS 187

Query: 486  SVELLGLNGFTIAISGLALAFGSFFSYLRVSQQLHTISQVVVGAAIGSVFSILWYWLWNA 307
             VE LGLNGFTIAISGL L FGSF SYLRV QQLHT+SQVVVGAAIGS+ SIL YWLWN 
Sbjct: 188  GVEWLGLNGFTIAISGLVLTFGSFLSYLRVVQQLHTVSQVVVGAAIGSISSILRYWLWNG 247

Query: 306  IVLDAFASSLWVRIIVVLGSAGLCLGFVVFAIRHWLRDD 190
             +LDAF SSLWVRIIVVLGSAGLC+GFV+F IRHWL+DD
Sbjct: 248  YMLDAFVSSLWVRIIVVLGSAGLCIGFVLFVIRHWLQDD 286


>XP_014519971.1 PREDICTED: lipid phosphate phosphatase epsilon 2, chloroplastic
            [Vigna radiata var. radiata]
          Length = 286

 Score =  395 bits (1015), Expect = e-134
 Identities = 197/279 (70%), Positives = 220/279 (78%)
 Frame = -3

Query: 1026 CYTPSYLVQRPNLLRQRHLKNTXXXXXXXXXXXFLCGGFVRRKLVFERYTLWVPNTMNES 847
            C TP  L Q  N L++R+LKNT            L  GF+ R+ +  R + WV  TM+ES
Sbjct: 8    CQTPFTLFQGSNCLKRRNLKNTSLPRCFSPSRSLLYTGFISRRPLLRRDSFWVSKTMDES 67

Query: 846  TRISAFRDGEGDENIQVFEQEALIDGSSVSRPMFLSREMEDTLNRLSKWIVAAFFGGFIL 667
             R SAFRDGEGDENIQVFEQEA IDG    +  FLS E+E  LNRLSKWIV A FG FIL
Sbjct: 68   VRTSAFRDGEGDENIQVFEQEAFIDGPPPFQSKFLSHEVEYKLNRLSKWIVTALFGSFIL 127

Query: 666  WRHDAEALWFTAGSILNALLSLWLKRILNQERPSTLKSDPGMPSSHAQSLFFSAVFVILS 487
            WRHDAEALWFT+GSILNALLS+ LKRILNQERPSTLKSDPGMPSSHAQS+FF+  FVILS
Sbjct: 128  WRHDAEALWFTSGSILNALLSVLLKRILNQERPSTLKSDPGMPSSHAQSIFFAVFFVILS 187

Query: 486  SVELLGLNGFTIAISGLALAFGSFFSYLRVSQQLHTISQVVVGAAIGSVFSILWYWLWNA 307
             VE LGLN FTIA+SGL L FGSFFSYLRVSQQLHT+SQVVVGA IGS+FSILWYWLWN 
Sbjct: 188  GVEWLGLNVFTIAMSGLVLTFGSFFSYLRVSQQLHTVSQVVVGAVIGSIFSILWYWLWNG 247

Query: 306  IVLDAFASSLWVRIIVVLGSAGLCLGFVVFAIRHWLRDD 190
             +LD F SSLWVRI+VVLGS GLC+GFV+F IR+WL+DD
Sbjct: 248  FILDVFVSSLWVRIVVVLGSVGLCIGFVLFTIRYWLQDD 286


>XP_007156981.1 hypothetical protein PHAVU_002G033600g [Phaseolus vulgaris]
            ESW28975.1 hypothetical protein PHAVU_002G033600g
            [Phaseolus vulgaris]
          Length = 287

 Score =  386 bits (991), Expect = e-130
 Identities = 195/280 (69%), Positives = 220/280 (78%), Gaps = 1/280 (0%)
 Frame = -3

Query: 1026 CYTPSYLVQRPNLLRQRHLKN-TXXXXXXXXXXXFLCGGFVRRKLVFERYTLWVPNTMNE 850
            C TP  L Q  N + +R+LKN +            LC GF  R+ V  R + WV  +M E
Sbjct: 8    CQTPFTLFQGSNGVERRNLKNASVPDCCFPPSRSLLCSGFHSRRPVLRRNSFWVSKSMGE 67

Query: 849  STRISAFRDGEGDENIQVFEQEALIDGSSVSRPMFLSREMEDTLNRLSKWIVAAFFGGFI 670
            S R SAFRDGEGDENIQVFEQEA IDG+S  +   LS E+E  LNRLSKWIV+A +GGFI
Sbjct: 68   SMRTSAFRDGEGDENIQVFEQEAFIDGTSPFQSKLLSHEVEYKLNRLSKWIVSALYGGFI 127

Query: 669  LWRHDAEALWFTAGSILNALLSLWLKRILNQERPSTLKSDPGMPSSHAQSLFFSAVFVIL 490
            LWRHDAEALWFTAGSILNALLS+ LK ILNQERPSTLKSDPGMPSSHAQS+FF+  FVIL
Sbjct: 128  LWRHDAEALWFTAGSILNALLSVLLKHILNQERPSTLKSDPGMPSSHAQSIFFAVFFVIL 187

Query: 489  SSVELLGLNGFTIAISGLALAFGSFFSYLRVSQQLHTISQVVVGAAIGSVFSILWYWLWN 310
            S VE LGLN FTIA+SGL L  GSFFSYLRVSQQLHT++QVVVGA IGS+FSILWYWLWN
Sbjct: 188  SGVEWLGLNVFTIALSGLVLTSGSFFSYLRVSQQLHTVNQVVVGAVIGSIFSILWYWLWN 247

Query: 309  AIVLDAFASSLWVRIIVVLGSAGLCLGFVVFAIRHWLRDD 190
              +LD FAS LWVRI+VVLGS GLC+GFV+FAIR+WL+DD
Sbjct: 248  GFILDVFASYLWVRIVVVLGSVGLCIGFVLFAIRYWLQDD 287


>KYP66800.1 hypothetical protein KK1_013111 [Cajanus cajan]
          Length = 282

 Score =  381 bits (979), Expect = e-129
 Identities = 195/276 (70%), Positives = 217/276 (78%)
 Frame = -3

Query: 1017 PSYLVQRPNLLRQRHLKNTXXXXXXXXXXXFLCGGFVRRKLVFERYTLWVPNTMNESTRI 838
            PS + Q  NLL+QR+LK T            L  GFV RKLVFER  LWV  TM    R 
Sbjct: 11   PSSVFQASNLLKQRYLKATSFAHTFSPSRSLLSTGFVPRKLVFERRHLWVSETM----RT 66

Query: 837  SAFRDGEGDENIQVFEQEALIDGSSVSRPMFLSREMEDTLNRLSKWIVAAFFGGFILWRH 658
            SA RDGEGDENIQV EQEA I+GSS     FLS E+E  LNRLSKWIV   FG FI+ RH
Sbjct: 67   SASRDGEGDENIQVLEQEAFINGSSQFLSNFLSHELEYKLNRLSKWIVTTLFGVFIVARH 126

Query: 657  DAEALWFTAGSILNALLSLWLKRILNQERPSTLKSDPGMPSSHAQSLFFSAVFVILSSVE 478
            DAEALWF AGSILNALLS+WLKRILNQERPSTLKSDPGMPSSH+QS+FF+  FVILSS+E
Sbjct: 127  DAEALWFAAGSILNALLSVWLKRILNQERPSTLKSDPGMPSSHSQSIFFTVFFVILSSIE 186

Query: 477  LLGLNGFTIAISGLALAFGSFFSYLRVSQQLHTISQVVVGAAIGSVFSILWYWLWNAIVL 298
             LGLNGFTIAISGL L FGSFFSYLRVSQQLHT+SQV VGA IGS+F  LWYWLW  ++L
Sbjct: 187  WLGLNGFTIAISGLVLTFGSFFSYLRVSQQLHTVSQVGVGAVIGSIFCFLWYWLWKGLML 246

Query: 297  DAFASSLWVRIIVVLGSAGLCLGFVVFAIRHWLRDD 190
            DAF S LWVR++V+LGSAG+CLGFV+FAIR+WL+DD
Sbjct: 247  DAFVSYLWVRVVVILGSAGICLGFVLFAIRYWLQDD 282


>AFK49290.1 unknown [Lotus japonicus]
          Length = 286

 Score =  372 bits (954), Expect = e-125
 Identities = 196/286 (68%), Positives = 221/286 (77%), Gaps = 7/286 (2%)
 Frame = -3

Query: 1026 CYTPSYLVQRPNLLRQRHLKN------TXXXXXXXXXXXFLCGGFVRRKLVFERYTLWVP 865
            C+ PS ++ RP LL+QR+LK       +           FL G FV +KL   R T WV 
Sbjct: 8    CFHPSSILHRPVLLKQRYLKKNTTLFASARSHSFSASRSFLSGEFVPKKLSLGRNTNWVS 67

Query: 864  NTMNESTRISAFRDG-EGDENIQVFEQEALIDGSSVSRPMFLSREMEDTLNRLSKWIVAA 688
            + MN S    A RDG   DENIQVFEQEALID  S     FLS++ME  LNRLSKWIVAA
Sbjct: 68   SPMNAS----ASRDGGNSDENIQVFEQEALIDAGSSK---FLSQDMEYKLNRLSKWIVAA 120

Query: 687  FFGGFILWRHDAEALWFTAGSILNALLSLWLKRILNQERPSTLKSDPGMPSSHAQSLFFS 508
             F G ILWRHD EALWF AGS+LNA+LS+WLKRILNQERPST+KSDPGMPSSHAQS+FF+
Sbjct: 121  LFAGLILWRHDVEALWFGAGSVLNAVLSVWLKRILNQERPSTVKSDPGMPSSHAQSIFFT 180

Query: 507  AVFVILSSVELLGLNGFTIAISGLALAFGSFFSYLRVSQQLHTISQVVVGAAIGSVFSIL 328
             +FVILSS+E LGLNGFTIAISGL LAFGSFFSYLRVSQQLHT SQV+VG AIGS++SIL
Sbjct: 181  VIFVILSSIEWLGLNGFTIAISGLVLAFGSFFSYLRVSQQLHTTSQVLVGGAIGSIYSIL 240

Query: 327  WYWLWNAIVLDAFASSLWVRIIVVLGSAGLCLGFVVFAIRHWLRDD 190
            WYWLW   VLDAF +SLWVRIIVVLGSAGLCLGFV+FA RHWLR++
Sbjct: 241  WYWLWKHFVLDAFIASLWVRIIVVLGSAGLCLGFVIFAFRHWLRNE 286


>XP_019444786.1 PREDICTED: lipid phosphate phosphatase epsilon 1, chloroplastic
           [Lupinus angustifolius]
          Length = 283

 Score =  347 bits (889), Expect = e-115
 Identities = 173/245 (70%), Positives = 197/245 (80%)
 Frame = -3

Query: 924 LCGGFVRRKLVFERYTLWVPNTMNESTRISAFRDGEGDENIQVFEQEALIDGSSVSRPMF 745
           LCGGF+ +     R  LWV N M++S    AF D + +ENIQVFEQEA ID S+     F
Sbjct: 43  LCGGFIPKTTTLGRNRLWVSNAMSDS----AFSDDKSNENIQVFEQEAFIDDSTQFCAKF 98

Query: 744 LSREMEDTLNRLSKWIVAAFFGGFILWRHDAEALWFTAGSILNALLSLWLKRILNQERPS 565
           LS ++E TLNRLSKWIV    G FILWRHD EALWF AGS+LN LLS+WLK ILNQERP+
Sbjct: 99  LSPQLESTLNRLSKWIVTVLMGCFILWRHDVEALWFGAGSVLNGLLSVWLKHILNQERPT 158

Query: 564 TLKSDPGMPSSHAQSLFFSAVFVILSSVELLGLNGFTIAISGLALAFGSFFSYLRVSQQL 385
           TLKSDPGMPSSHAQS+FF+A FVILSSVE LGLN FTI +SGL LA  SFFSYLRVSQ+L
Sbjct: 159 TLKSDPGMPSSHAQSIFFAAFFVILSSVEWLGLNVFTIFVSGLVLASASFFSYLRVSQKL 218

Query: 384 HTISQVVVGAAIGSVFSILWYWLWNAIVLDAFASSLWVRIIVVLGSAGLCLGFVVFAIRH 205
           HT+SQVVVGAAIGS+FS LWYWLWNA +L AF SS+WVRIIVVLGSAGLCLGF +FAI H
Sbjct: 219 HTMSQVVVGAAIGSIFSTLWYWLWNAFLLHAFVSSIWVRIIVVLGSAGLCLGFFLFAIPH 278

Query: 204 WLRDD 190
           WL+++
Sbjct: 279 WLQNE 283


>XP_013446388.1 phosphatidic acid phosphatase (PAP2) family protein [Medicago
            truncatula] KEH20415.1 phosphatidic acid phosphatase
            (PAP2) family protein [Medicago truncatula]
          Length = 283

 Score =  335 bits (859), Expect = e-110
 Identities = 170/279 (60%), Positives = 208/279 (74%)
 Frame = -3

Query: 1026 CYTPSYLVQRPNLLRQRHLKNTXXXXXXXXXXXFLCGGFVRRKLVFERYTLWVPNTMNES 847
            CY PS    R N  +QR+LKN+           F CG        F  + LWV + M+  
Sbjct: 14   CYNPSNNFLRTNQPKQRYLKNSSFSPSLSASRSFTCG--------FVPFNLWVKSAMDGF 65

Query: 846  TRISAFRDGEGDENIQVFEQEALIDGSSVSRPMFLSREMEDTLNRLSKWIVAAFFGGFIL 667
             R +A RDG+ D +I+V EQEA +D SS S P FL +E+E TLNRLSKWIV+A F  F++
Sbjct: 66   VR-TANRDGKSDGHIEVLEQEAFVDRSSESEPKFLFQEVESTLNRLSKWIVSALFAVFVI 124

Query: 666  WRHDAEALWFTAGSILNALLSLWLKRILNQERPSTLKSDPGMPSSHAQSLFFSAVFVILS 487
            WRHDAEALWF  GS+LNA+LS+ LK++LNQ+RPST+KSDPGMPSSH+QS+FF+ +F ILS
Sbjct: 125  WRHDAEALWFAGGSVLNAVLSVLLKQLLNQKRPSTVKSDPGMPSSHSQSIFFAVMFSILS 184

Query: 486  SVELLGLNGFTIAISGLALAFGSFFSYLRVSQQLHTISQVVVGAAIGSVFSILWYWLWNA 307
            SVELL +N  TI  S LALAFGS+ SYLRVSQ+LHT+SQVVVGA +GS+FSILWYWLWNA
Sbjct: 185  SVELLSINALTITSSCLALAFGSYLSYLRVSQKLHTVSQVVVGAVVGSMFSILWYWLWNA 244

Query: 306  IVLDAFASSLWVRIIVVLGSAGLCLGFVVFAIRHWLRDD 190
             VLDAF SSLWVRIIVVL SA  CLGF++  +RHWL+D+
Sbjct: 245  FVLDAFVSSLWVRIIVVLVSAVFCLGFLLHVVRHWLKDE 283


>XP_004509286.1 PREDICTED: lipid phosphate phosphatase epsilon 1, chloroplastic
            isoform X1 [Cicer arietinum]
          Length = 284

 Score =  331 bits (848), Expect = e-109
 Identities = 165/281 (58%), Positives = 206/281 (73%)
 Frame = -3

Query: 1032 ICCYTPSYLVQRPNLLRQRHLKNTXXXXXXXXXXXFLCGGFVRRKLVFERYTLWVPNTMN 853
            I CY PS      N L+QR+LK             F CG        F  + L   + M+
Sbjct: 13   ILCYNPSSKFLGTNPLKQRYLKKNSFSPSLSGSRSFTCG--------FVLFNLRGKSAMD 64

Query: 852  ESTRISAFRDGEGDENIQVFEQEALIDGSSVSRPMFLSREMEDTLNRLSKWIVAAFFGGF 673
               R +A+RDG  DE++QV EQEA +  SS  +P FL +EME TLN+LSKWIV A FG F
Sbjct: 65   GFER-TAYRDGNSDEHVQVLEQEAFVGSSSELQPKFLFQEMESTLNQLSKWIVTALFGAF 123

Query: 672  ILWRHDAEALWFTAGSILNALLSLWLKRILNQERPSTLKSDPGMPSSHAQSLFFSAVFVI 493
            ++WRHDAEALWF  GS+LNA+LS+ LK+ILNQ+RPSTLKSDPGMPSSH+QS+FF  +F+I
Sbjct: 124  VIWRHDAEALWFAGGSVLNAMLSVLLKQILNQKRPSTLKSDPGMPSSHSQSIFFGVMFII 183

Query: 492  LSSVELLGLNGFTIAISGLALAFGSFFSYLRVSQQLHTISQVVVGAAIGSVFSILWYWLW 313
            LSSVE L +N FTI  S LALA GS+ SYLRVSQ+LHT+SQV+VGA +GS+ SILWYWLW
Sbjct: 184  LSSVESLSINAFTITSSCLALALGSYMSYLRVSQKLHTVSQVIVGAFVGSICSILWYWLW 243

Query: 312  NAIVLDAFASSLWVRIIVVLGSAGLCLGFVVFAIRHWLRDD 190
            N  +L+AF SSLWVRIIV+LGSAG+CLGF+++ IRHWL+++
Sbjct: 244  NGFMLNAFVSSLWVRIIVILGSAGICLGFLLYVIRHWLKEE 284


>XP_016201895.1 PREDICTED: lipid phosphate phosphatase epsilon 1,
           chloroplastic-like [Arachis ipaensis]
          Length = 293

 Score =  315 bits (807), Expect = e-102
 Identities = 168/274 (61%), Positives = 197/274 (71%), Gaps = 4/274 (1%)
 Frame = -3

Query: 999 RPNLLRQRH--LKNTXXXXXXXXXXXFLCGGFVRRKLVFERYTLWVP-NTMNESTRISAF 829
           R  LLR  H   KNT            L GG V R        +WV  NTMNE T+ SAF
Sbjct: 20  RTTLLRHTHPFKKNTSFALTFSASRSVLFGGAVTRTHFLGGNKIWVSSNTMNEFTQPSAF 79

Query: 828 RDGEGDENIQVFEQEALIDGSSVSRPMFLSREMEDTLNRLSKWIVAAFFGGFILWRHDAE 649
           RD E D+  QVFEQE+ IDGS+  +P  L R++E  LNRLSKWIVA  FGGFILW HD E
Sbjct: 80  RDRERDDIKQVFEQESFIDGSTEVQPNILFRDIEPRLNRLSKWIVAVSFGGFILWSHDIE 139

Query: 648 -ALWFTAGSILNALLSLWLKRILNQERPSTLKSDPGMPSSHAQSLFFSAVFVILSSVELL 472
            ALW  AGSILN+ LS+ LK ILNQERPS LKSDPGMPS+HAQS+FF   F I S+VELL
Sbjct: 140 EALWIFAGSILNSFLSVLLKNILNQERPSALKSDPGMPSTHAQSIFFVVFFFIFSTVELL 199

Query: 471 GLNGFTIAISGLALAFGSFFSYLRVSQQLHTISQVVVGAAIGSVFSILWYWLWNAIVLDA 292
           GLN ++I +SGL L  GS+FSYLRVSQQLHT++QVVVGA IGS+ S++WYWLWNA + DA
Sbjct: 200 GLNVYSIVLSGLYLTCGSYFSYLRVSQQLHTMNQVVVGATIGSIMSLVWYWLWNAFIQDA 259

Query: 291 FASSLWVRIIVVLGSAGLCLGFVVFAIRHWLRDD 190
           + SSL+VRII + GS G+C+ FVVF IRHWL+DD
Sbjct: 260 YQSSLFVRIIGLSGSIGICICFVVFVIRHWLKDD 293


>XP_015964140.1 PREDICTED: lipid phosphate phosphatase epsilon 1,
           chloroplastic-like [Arachis duranensis]
          Length = 288

 Score =  315 bits (806), Expect = e-102
 Identities = 167/274 (60%), Positives = 197/274 (71%), Gaps = 4/274 (1%)
 Frame = -3

Query: 999 RPNLLRQRH--LKNTXXXXXXXXXXXFLCGGFVRRKLVFERYTLWVP-NTMNESTRISAF 829
           R  LLR  H   KNT            L GG V R        +WV  NTMNE T+ SAF
Sbjct: 15  RTTLLRHTHPFKKNTSFALTFSASRSVLFGGAVTRTHFLGGNKIWVSSNTMNEFTQPSAF 74

Query: 828 RDGEGDENIQVFEQEALIDGSSVSRPMFLSREMEDTLNRLSKWIVAAFFGGFILWRHDAE 649
           RD E D+  QVFEQEA IDGS+  +P  L R++E  LNRLSKWIVA  FGGFILW HD E
Sbjct: 75  RDRERDDIKQVFEQEAFIDGSTEFQPNILFRDIEPRLNRLSKWIVAVSFGGFILWSHDIE 134

Query: 648 -ALWFTAGSILNALLSLWLKRILNQERPSTLKSDPGMPSSHAQSLFFSAVFVILSSVELL 472
            ALW  AGSILN+ LS+ LK ILNQERPS LKSDPGMPS+HAQS+FF   F I S++ELL
Sbjct: 135 EALWIFAGSILNSFLSVLLKNILNQERPSALKSDPGMPSTHAQSIFFVVFFFIFSTIELL 194

Query: 471 GLNGFTIAISGLALAFGSFFSYLRVSQQLHTISQVVVGAAIGSVFSILWYWLWNAIVLDA 292
           GLN ++I +SGL L  GS+FSYLRVSQQLHT++QVVVGA IGS+ S++WYWLWNA + DA
Sbjct: 195 GLNVYSIVLSGLYLTCGSYFSYLRVSQQLHTMNQVVVGATIGSIMSLVWYWLWNAFIQDA 254

Query: 291 FASSLWVRIIVVLGSAGLCLGFVVFAIRHWLRDD 190
           + SSL+VRII + GS G+C+ F+VF IRHWL+DD
Sbjct: 255 YQSSLFVRIIGLSGSIGICICFLVFVIRHWLKDD 288


>GAU36018.1 hypothetical protein TSUD_211560 [Trifolium subterraneum]
          Length = 300

 Score =  308 bits (789), Expect = e-99
 Identities = 155/265 (58%), Positives = 195/265 (73%)
 Frame = -3

Query: 1026 CYTPSYLVQRPNLLRQRHLKNTXXXXXXXXXXXFLCGGFVRRKLVFERYTLWVPNTMNES 847
            CY PS    R N  ++R+L+N                GF+  KL       WV + M+  
Sbjct: 9    CYNPSNNFSRTNQPKRRYLRNNSFSPDLFTSRS-FTSGFLPDKL-------WVKSAMDGF 60

Query: 846  TRISAFRDGEGDENIQVFEQEALIDGSSVSRPMFLSREMEDTLNRLSKWIVAAFFGGFIL 667
             R +A R+G  DE+I+V EQEA +D SS  +  +  +++E TLNRL+KWIV+A FG F++
Sbjct: 61   VRTAAHREGMTDEHIEVLEQEAFVDRSSELQQKYFFQDVEATLNRLTKWIVSALFGVFVI 120

Query: 666  WRHDAEALWFTAGSILNALLSLWLKRILNQERPSTLKSDPGMPSSHAQSLFFSAVFVILS 487
            WRHDAEALWF  GS++NA+LS+ LK+ILNQ+RPST+KSDPGMPSSH+QS+FF+ +F+ILS
Sbjct: 121  WRHDAEALWFAGGSVVNAILSVLLKQILNQKRPSTVKSDPGMPSSHSQSIFFAVMFIILS 180

Query: 486  SVELLGLNGFTIAISGLALAFGSFFSYLRVSQQLHTISQVVVGAAIGSVFSILWYWLWNA 307
            SVELL +N FTI  S LALAFGS+ SYLRVSQ+LHT+SQVVVGA +GS+FSILWYWLWNA
Sbjct: 181  SVELLTINAFTITSSVLALAFGSYLSYLRVSQKLHTVSQVVVGAVVGSIFSILWYWLWNA 240

Query: 306  IVLDAFASSLWVRIIVVLGSAGLCL 232
             VLDAF SSLWVRIIVVLGS   C+
Sbjct: 241  FVLDAFVSSLWVRIIVVLGSVAFCI 265


>AFK34692.1 unknown [Lotus japonicus]
          Length = 278

 Score =  297 bits (761), Expect = 8e-96
 Identities = 161/279 (57%), Positives = 192/279 (68%), Gaps = 3/279 (1%)
 Frame = -3

Query: 1023 YTPSYLVQRPNLL-RQRHLKNTXXXXXXXXXXXFL-CGGFVRRKLVFERYTLWVPNTMNE 850
            Y PS      NL  + R LKN+            L  GGFV  KL          N M++
Sbjct: 10   YNPSNNFLGTNLQHKHRLLKNSSSFARRFSNSRTLFLGGFVGDKLC-------AANAMDD 62

Query: 849  STR-ISAFRDGEGDENIQVFEQEALIDGSSVSRPMFLSREMEDTLNRLSKWIVAAFFGGF 673
              +   A RD + D  +QV EQEA IDGSS   P FL  E+E TLN++SKW+V A FG F
Sbjct: 63   GFKGACAHRDEKSDGQVQVVEQEAFIDGSSEFHPKFLFHELESTLNQMSKWLVTAIFGVF 122

Query: 672  ILWRHDAEALWFTAGSILNALLSLWLKRILNQERPSTLKSDPGMPSSHAQSLFFSAVFVI 493
            ILWRHDAEALWF AGS LNA+LS  LK++LNQ+RPSTLKSDPGMPSSHAQS+FF  +F+I
Sbjct: 123  ILWRHDAEALWFAAGSFLNAMLSASLKQMLNQKRPSTLKSDPGMPSSHAQSIFFIVLFII 182

Query: 492  LSSVELLGLNGFTIAISGLALAFGSFFSYLRVSQQLHTISQVVVGAAIGSVFSILWYWLW 313
            LS     G+N FTI  SGLALAF S+FSYLRVSQ+LHT+SQV+VGA  GS+ +ILW WLW
Sbjct: 183  LS-----GMNEFTITSSGLALAFASYFSYLRVSQKLHTVSQVIVGAVTGSICAILWQWLW 237

Query: 312  NAIVLDAFASSLWVRIIVVLGSAGLCLGFVVFAIRHWLR 196
            N+ +LD F SS WVRIIVVLGS G CLGF++  I+HWL+
Sbjct: 238  NSFMLDVFVSSSWVRIIVVLGSVGFCLGFLLHVIQHWLK 276


>XP_002263902.1 PREDICTED: lipid phosphate phosphatase epsilon 1, chloroplastic
           [Vitis vinifera] CAN70630.1 hypothetical protein
           VITISV_020724 [Vitis vinifera]
          Length = 283

 Score =  292 bits (748), Expect = 9e-94
 Identities = 145/244 (59%), Positives = 181/244 (74%), Gaps = 1/244 (0%)
 Frame = -3

Query: 918 GGFVRRKLVFERYTLWVPNTMNESTRISAFRDGEGDENIQVFEQEALIDGSSVSRPMFLS 739
           GGF   K +F +  +  P TM E  R SAFR+G  DE   + E+EA I GSS      ++
Sbjct: 40  GGFSSEKFLFRKNRVGGPLTMTELVRTSAFRNGNDDEGATMIEEEAFITGSSEFPADIVA 99

Query: 738 REMEDTLNRLSKWIVAAFFGGFILWRHDAEALWFTAGSILNALLSLWLKRILNQERP-ST 562
             +E TLNRLSKW+VAA FG  ILWRHDAE+LW   GS+LN +LS+ LK+ILNQERP S 
Sbjct: 100 GGLEATLNRLSKWLVAALFGIVILWRHDAESLWAAMGSVLNTVLSVTLKQILNQERPVSA 159

Query: 561 LKSDPGMPSSHAQSLFFSAVFVILSSVELLGLNGFTIAISGLALAFGSFFSYLRVSQQLH 382
           L+S PGMPSSHAQS+FF+ VF ILS VE LG+NG T+ ISGLALA GS+ S+LRVSQQ H
Sbjct: 160 LRSGPGMPSSHAQSIFFTVVFTILSVVEWLGINGLTLTISGLALALGSYLSWLRVSQQFH 219

Query: 381 TISQVVVGAAIGSVFSILWYWLWNAIVLDAFASSLWVRIIVVLGSAGLCLGFVVFAIRHW 202
           TISQV+VG+A+GSVF ILW W W A VL+A+ S LWVR++V++G+ G CLGFV+  I+HW
Sbjct: 220 TISQVLVGSAVGSVFCILWLWSWEAFVLNAYTSYLWVRVLVIVGAVGFCLGFVLHVIKHW 279

Query: 201 LRDD 190
           L ++
Sbjct: 280 LLEE 283


>XP_002521546.1 PREDICTED: lipid phosphate phosphatase epsilon 2, chloroplastic
           [Ricinus communis] EEF40817.1 conserved hypothetical
           protein [Ricinus communis]
          Length = 279

 Score =  289 bits (739), Expect = 2e-92
 Identities = 140/228 (61%), Positives = 171/228 (75%), Gaps = 1/228 (0%)
 Frame = -3

Query: 870 VPNTMNESTRISAFRDGEGDENIQVFEQEALIDGSSVSRPMFLSREMEDTLNRLSKWIVA 691
           V  TM E    SAFR G+G+EN+++ + + L D SS  R   ++R  E  LNRLSKW+VA
Sbjct: 51  VSKTMTEMVWTSAFRSGDGEENVKILQPDTLADDSSDFRSQLMARGFEPLLNRLSKWLVA 110

Query: 690 AFFGGFILWRHDAEALWFTAGSILNALLSLWLKRILNQERP-STLKSDPGMPSSHAQSLF 514
           A FG  +LWRHDAEALW   GS+LNA+LS+ LKR+ NQERP +T KSDPGMPSSHAQ +F
Sbjct: 111 ALFGVVLLWRHDAEALWIAMGSVLNAILSVTLKRVFNQERPFATAKSDPGMPSSHAQCIF 170

Query: 513 FSAVFVILSSVELLGLNGFTIAISGLALAFGSFFSYLRVSQQLHTISQVVVGAAIGSVFS 334
           ++ VF  LS  E LG+N FT+ ISGL LA GS+FS+LRVSQQ HT SQVVVGAA GS+FS
Sbjct: 171 YTVVFCSLSVTEWLGVNEFTLLISGLNLALGSYFSWLRVSQQYHTASQVVVGAAAGSLFS 230

Query: 333 ILWYWLWNAIVLDAFASSLWVRIIVVLGSAGLCLGFVVFAIRHWLRDD 190
            LWYWLW  IVLD F SSLWVRI+VV  +   CLGF+++ IRHWL+D+
Sbjct: 231 FLWYWLWQTIVLDEFNSSLWVRIVVVFAAFSFCLGFLLYVIRHWLKDE 278


>XP_018829479.1 PREDICTED: lipid phosphate phosphatase epsilon 2,
           chloroplastic-like [Juglans regia]
          Length = 288

 Score =  288 bits (736), Expect = 7e-92
 Identities = 145/244 (59%), Positives = 179/244 (73%), Gaps = 1/244 (0%)
 Frame = -3

Query: 918 GGFVRRKLVFERYTLWVPNTMNESTRISAFRDGEGDENIQVFEQEALIDGSSVSRPMFLS 739
           GGFV  K   +R      N M E  ++SAF+ G+ DE I V  QE + DGSS     F++
Sbjct: 43  GGFVSSKSFCDRNRFKGTNPMTELIKVSAFKGGDHDEGISVLSQETIADGSSRFPQEFMA 102

Query: 738 REMEDTLNRLSKWIVAAFFGGFILWRHDAEALWFTAGSILNALLSLWLKRILNQERP-ST 562
             +E TLNR+SKW+VAA FGG IL RHD+EALW   GS++N+  SL LK+ILNQERP ST
Sbjct: 103 DGLEPTLNRMSKWLVAALFGGVILLRHDSEALWAAMGSVVNSAFSLVLKQILNQERPVST 162

Query: 561 LKSDPGMPSSHAQSLFFSAVFVILSSVELLGLNGFTIAISGLALAFGSFFSYLRVSQQLH 382
           L+SDPGMPSSHAQS+FF+  F ILS VE LG+N FT+ IS L LAFGS+ ++LRVSQQ H
Sbjct: 163 LRSDPGMPSSHAQSIFFTVGFSILSIVEWLGINDFTVIISVLVLAFGSYLTWLRVSQQFH 222

Query: 381 TISQVVVGAAIGSVFSILWYWLWNAIVLDAFASSLWVRIIVVLGSAGLCLGFVVFAIRHW 202
           T+SQVVVGA +GS+F ILW W W+A VL AF SSLWVRI+VVL + G CLGF+V  +R+W
Sbjct: 223 TVSQVVVGAVLGSIFCILWLWSWDAFVLQAFDSSLWVRIVVVLVAVGFCLGFLVHVVRNW 282

Query: 201 LRDD 190
            RD+
Sbjct: 283 FRDE 286