BLASTX nr result
ID: Glycyrrhiza35_contig00039513
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Glycyrrhiza35_contig00039513
(386 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
XP_007146252.1 hypothetical protein PHAVU_006G0250000g, partial ... 192 8e-58
KYP42310.1 hypothetical protein KK1_036302 [Cajanus cajan] 185 1e-56
XP_007147219.1 hypothetical protein PHAVU_006G105700g [Phaseolus... 192 1e-55
KYP63377.1 hypothetical protein KK1_017946 [Cajanus cajan] 181 2e-55
KYP32294.1 hypothetical protein KK1_047062, partial [Cajanus cajan] 176 2e-52
XP_007153622.1 hypothetical protein PHAVU_003G051000g [Phaseolus... 181 6e-52
KYP34861.1 hypothetical protein KK1_044131, partial [Cajanus cajan] 173 1e-51
XP_007135196.1 hypothetical protein PHAVU_010G109100g [Phaseolus... 181 2e-51
XP_007132971.1 hypothetical protein PHAVU_011G140600g [Phaseolus... 180 3e-51
XP_016198607.1 PREDICTED: uncharacterized protein LOC107639580 [... 176 5e-50
XP_015949085.1 PREDICTED: uncharacterized protein LOC107474018 [... 175 6e-50
XP_015935521.1 PREDICTED: uncharacterized protein LOC107461514 [... 177 1e-49
XP_016192700.1 PREDICTED: uncharacterized protein LOC107633607, ... 173 3e-49
XP_019465412.1 PREDICTED: uncharacterized protein LOC109363616 [... 171 4e-49
XP_011010809.1 PREDICTED: uncharacterized protein LOC105115580 [... 170 5e-49
XP_016195810.1 PREDICTED: uncharacterized protein LOC107636851 [... 166 7e-49
XP_011001694.1 PREDICTED: uncharacterized protein LOC105108893 [... 170 2e-48
GAU21484.1 hypothetical protein TSUD_242060 [Trifolium subterran... 174 2e-48
XP_007154637.1 hypothetical protein PHAVU_003G135300g [Phaseolus... 173 4e-48
XP_007150061.1 hypothetical protein PHAVU_005G123000g, partial [... 171 5e-48
>XP_007146252.1 hypothetical protein PHAVU_006G0250000g, partial [Phaseolus
vulgaris] ESW18246.1 hypothetical protein
PHAVU_006G0250000g, partial [Phaseolus vulgaris]
Length = 388
Score = 192 bits (488), Expect = 8e-58
Identities = 88/108 (81%), Positives = 102/108 (94%)
Frame = +1
Query: 61 SYKAAGDLLMQKRKKLYWTPCAAHCIELMLEDFEKKIPLHKETIASGKKITTYIYSRSGL 240
+YKAAG+LLMQKRKKLYWTPCA HCI+LMLEDFEKKIPLH +TIA+GKKITTYIYSR+GL
Sbjct: 270 NYKAAGELLMQKRKKLYWTPCATHCIDLMLEDFEKKIPLHHDTIANGKKITTYIYSRTGL 329
Query: 241 VSLLHHFTKGKDLIRPAATRFATSYLTLGCLNDNKGALIRMFTSKAWK 384
+SLLH +++GKDLIRPA TRFATSYLTLGCLNDN+G+LI+MFTS W+
Sbjct: 330 ISLLHKYSEGKDLIRPANTRFATSYLTLGCLNDNRGSLIKMFTSLEWQ 377
>KYP42310.1 hypothetical protein KK1_036302 [Cajanus cajan]
Length = 248
Score = 185 bits (469), Expect = 1e-56
Identities = 81/108 (75%), Positives = 99/108 (91%)
Frame = +1
Query: 61 SYKAAGDLLMQKRKKLYWTPCAAHCIELMLEDFEKKIPLHKETIASGKKITTYIYSRSGL 240
+YKAAG +LM+KRK+L+WTPCAAHC++LMLED+EKKIP+H+ETI GK+ITT+IYSR+ L
Sbjct: 68 NYKAAGQMLMEKRKRLFWTPCAAHCVDLMLEDYEKKIPIHEETIPKGKRITTFIYSRTSL 127
Query: 241 VSLLHHFTKGKDLIRPAATRFATSYLTLGCLNDNKGALIRMFTSKAWK 384
+SLL HFTKG+DL+RP TRFATSYLTLGCL++NKGALIRMFTS WK
Sbjct: 128 ISLLQHFTKGRDLVRPGITRFATSYLTLGCLHENKGALIRMFTSNEWK 175
>XP_007147219.1 hypothetical protein PHAVU_006G105700g [Phaseolus vulgaris]
ESW19213.1 hypothetical protein PHAVU_006G105700g
[Phaseolus vulgaris]
Length = 682
Score = 192 bits (489), Expect = 1e-55
Identities = 88/108 (81%), Positives = 101/108 (93%)
Frame = +1
Query: 61 SYKAAGDLLMQKRKKLYWTPCAAHCIELMLEDFEKKIPLHKETIASGKKITTYIYSRSGL 240
+YKAAG+LLMQKRKKLYWTPCAAHCI++MLEDFEKKIPLH +TI +GKKITTYIYSR+GL
Sbjct: 274 NYKAAGELLMQKRKKLYWTPCAAHCIDMMLEDFEKKIPLHHDTIVNGKKITTYIYSRTGL 333
Query: 241 VSLLHHFTKGKDLIRPAATRFATSYLTLGCLNDNKGALIRMFTSKAWK 384
+SLLH ++ GKDLIRPA TRFATSYLTLGCLNDNKG+LI+MFTS W+
Sbjct: 334 ISLLHKYSDGKDLIRPANTRFATSYLTLGCLNDNKGSLIKMFTSSEWQ 381
>KYP63377.1 hypothetical protein KK1_017946 [Cajanus cajan]
Length = 237
Score = 181 bits (460), Expect = 2e-55
Identities = 83/107 (77%), Positives = 94/107 (87%)
Frame = +1
Query: 61 SYKAAGDLLMQKRKKLYWTPCAAHCIELMLEDFEKKIPLHKETIASGKKITTYIYSRSGL 240
+YKA G++LM+KR KLYWTPCAAHCI+L+LEDFEKKIPLH ETIASG+KIT+YIY R+ L
Sbjct: 68 NYKAVGEMLMKKRTKLYWTPCAAHCIDLILEDFEKKIPLHSETIASGRKITSYIYGRTSL 127
Query: 241 VSLLHHFTKGKDLIRPAATRFATSYLTLGCLNDNKGALIRMFTSKAW 381
+ LLH FTKG DLIRP TRFATSYLTLGCLN+NKG L RMFTSK W
Sbjct: 128 IVLLHKFTKGSDLIRPGLTRFATSYLTLGCLNENKGPLTRMFTSKEW 174
>KYP32294.1 hypothetical protein KK1_047062, partial [Cajanus cajan]
Length = 287
Score = 176 bits (445), Expect = 2e-52
Identities = 76/108 (70%), Positives = 96/108 (88%)
Frame = +1
Query: 61 SYKAAGDLLMQKRKKLYWTPCAAHCIELMLEDFEKKIPLHKETIASGKKITTYIYSRSGL 240
+YK AG +LM+KRK+L+WTPC+A+C++LMLED+EKKIP+H+ETI GK+I T+IYSR+ L
Sbjct: 62 NYKVAGQMLMEKRKRLFWTPCSAYCVDLMLEDYEKKIPIHEETIPKGKRIITFIYSRTSL 121
Query: 241 VSLLHHFTKGKDLIRPAATRFATSYLTLGCLNDNKGALIRMFTSKAWK 384
+SLL HFTKG+DL+RP T FATSYLTLGCL++NKGALIRMFTS WK
Sbjct: 122 ISLLQHFTKGRDLVRPGITHFATSYLTLGCLHENKGALIRMFTSNEWK 169
>XP_007153622.1 hypothetical protein PHAVU_003G051000g [Phaseolus vulgaris]
ESW25616.1 hypothetical protein PHAVU_003G051000g
[Phaseolus vulgaris]
Length = 568
Score = 181 bits (459), Expect = 6e-52
Identities = 86/104 (82%), Positives = 99/104 (95%)
Frame = +1
Query: 61 SYKAAGDLLMQKRKKLYWTPCAAHCIELMLEDFEKKIPLHKETIASGKKITTYIYSRSGL 240
+YKAAG+LLMQKRKKLY TP AAHCI+LMLEDFEKKIPLH +TIA+GKKITTYIYSR+GL
Sbjct: 233 NYKAAGELLMQKRKKLYLTPYAAHCIDLMLEDFEKKIPLHHDTIANGKKITTYIYSRTGL 292
Query: 241 VSLLHHFTKGKDLIRPAATRFATSYLTLGCLNDNKGALIRMFTS 372
+SLLH +++GKDLIRPA TRFATSYLTLGCLNDN+G+LI+MFTS
Sbjct: 293 ISLLHKYSEGKDLIRPANTRFATSYLTLGCLNDNRGSLIKMFTS 336
>KYP34861.1 hypothetical protein KK1_044131, partial [Cajanus cajan]
Length = 283
Score = 173 bits (439), Expect = 1e-51
Identities = 79/107 (73%), Positives = 92/107 (85%)
Frame = +1
Query: 61 SYKAAGDLLMQKRKKLYWTPCAAHCIELMLEDFEKKIPLHKETIASGKKITTYIYSRSGL 240
+YKAA ++LM+KR KLYWTPCA HCI+LMLEDFEKKI LH ETIA G++IT+YIY R+ L
Sbjct: 81 NYKAAREMLMKKRTKLYWTPCAVHCIDLMLEDFEKKIQLHSETIAIGRRITSYIYGRTSL 140
Query: 241 VSLLHHFTKGKDLIRPAATRFATSYLTLGCLNDNKGALIRMFTSKAW 381
++LLH FTKG DLIRP +T FATSYLTLGCLN+NKG L RMFTSK W
Sbjct: 141 IALLHKFTKGSDLIRPGSTHFATSYLTLGCLNENKGLLTRMFTSKKW 187
>XP_007135196.1 hypothetical protein PHAVU_010G109100g [Phaseolus vulgaris]
ESW07190.1 hypothetical protein PHAVU_010G109100g
[Phaseolus vulgaris]
Length = 610
Score = 181 bits (458), Expect = 2e-51
Identities = 84/108 (77%), Positives = 95/108 (87%)
Frame = +1
Query: 61 SYKAAGDLLMQKRKKLYWTPCAAHCIELMLEDFEKKIPLHKETIASGKKITTYIYSRSGL 240
+YKAAG+LLMQKRK LYWTPCA HCI LML+DFEKKIPLH ETIA+GKKI T+IYSRS L
Sbjct: 190 NYKAAGELLMQKRKNLYWTPCATHCIVLMLKDFEKKIPLHHETIANGKKIKTFIYSRSDL 249
Query: 241 VSLLHHFTKGKDLIRPAATRFATSYLTLGCLNDNKGALIRMFTSKAWK 384
+SLLH +T G DLI+P TRF TSYLTLGCLN+NKG+LIRMFTSK W+
Sbjct: 250 ISLLHKYTTGTDLIKPTNTRFVTSYLTLGCLNENKGSLIRMFTSKEWQ 297
>XP_007132971.1 hypothetical protein PHAVU_011G140600g [Phaseolus vulgaris]
ESW04965.1 hypothetical protein PHAVU_011G140600g
[Phaseolus vulgaris]
Length = 618
Score = 180 bits (457), Expect = 3e-51
Identities = 84/108 (77%), Positives = 96/108 (88%)
Frame = +1
Query: 61 SYKAAGDLLMQKRKKLYWTPCAAHCIELMLEDFEKKIPLHKETIASGKKITTYIYSRSGL 240
+YK GDLLMQKR+KLYWTPCA HCI+LMLEDFEKKIPLH+ETI++GKKITTYIYSR+ L
Sbjct: 220 NYKVVGDLLMQKRRKLYWTPCATHCIDLMLEDFEKKIPLHQETISNGKKITTYIYSRTSL 279
Query: 241 VSLLHHFTKGKDLIRPAATRFATSYLTLGCLNDNKGALIRMFTSKAWK 384
+ LLH T+G DLIR A TRFATSYLTLGCLN+NK +LIRMFTSK W+
Sbjct: 280 ICLLHKHTEGIDLIRSANTRFATSYLTLGCLNENKKSLIRMFTSKEWQ 327
>XP_016198607.1 PREDICTED: uncharacterized protein LOC107639580 [Arachis ipaensis]
Length = 566
Score = 176 bits (446), Expect = 5e-50
Identities = 79/107 (73%), Positives = 95/107 (88%)
Frame = +1
Query: 61 SYKAAGDLLMQKRKKLYWTPCAAHCIELMLEDFEKKIPLHKETIASGKKITTYIYSRSGL 240
+YKAAG++LM+KRKKL+WTPCAAHCI+LMLED EKK+ LHK+TI G+KITTYIY+R+ L
Sbjct: 311 NYKAAGEMLMKKRKKLFWTPCAAHCIDLMLEDLEKKVTLHKDTIYKGRKITTYIYARTAL 370
Query: 241 VSLLHHFTKGKDLIRPAATRFATSYLTLGCLNDNKGALIRMFTSKAW 381
++LLH TKGKDL+RP TRFATSYLTLGCLNDNKG+LI+MF S W
Sbjct: 371 IALLHIHTKGKDLVRPGMTRFATSYLTLGCLNDNKGSLIKMFLSDQW 417
>XP_015949085.1 PREDICTED: uncharacterized protein LOC107474018 [Arachis
duranensis]
Length = 521
Score = 175 bits (443), Expect = 6e-50
Identities = 78/107 (72%), Positives = 95/107 (88%)
Frame = +1
Query: 61 SYKAAGDLLMQKRKKLYWTPCAAHCIELMLEDFEKKIPLHKETIASGKKITTYIYSRSGL 240
+YKAAG++LM+KRK L+WTPCAAHCI+LMLED EKK+ +HK+TI G+KITTYIYSR+ L
Sbjct: 213 NYKAAGEMLMKKRKNLFWTPCAAHCIDLMLEDLEKKVTIHKDTIYKGRKITTYIYSRNAL 272
Query: 241 VSLLHHFTKGKDLIRPAATRFATSYLTLGCLNDNKGALIRMFTSKAW 381
++LLH TKGKDL+RP+ TRFATSYLTLGCLNDNKG+LI+MF S W
Sbjct: 273 IALLHIHTKGKDLVRPSMTRFATSYLTLGCLNDNKGSLIKMFLSDQW 319
>XP_015935521.1 PREDICTED: uncharacterized protein LOC107461514 [Arachis
duranensis]
Length = 728
Score = 177 bits (449), Expect = 1e-49
Identities = 80/107 (74%), Positives = 95/107 (88%)
Frame = +1
Query: 61 SYKAAGDLLMQKRKKLYWTPCAAHCIELMLEDFEKKIPLHKETIASGKKITTYIYSRSGL 240
+YKAAG++LM+KRKKL+WTPCAAHCI+LMLED EKK+ LHK+TI G+KITTYIY+R+ L
Sbjct: 281 NYKAAGEMLMKKRKKLFWTPCAAHCIDLMLEDLEKKVTLHKDTIYKGRKITTYIYARTAL 340
Query: 241 VSLLHHFTKGKDLIRPAATRFATSYLTLGCLNDNKGALIRMFTSKAW 381
++LLH TKGKDL+RP TRFATSYLTLGCLNDNKG+LIRMF S W
Sbjct: 341 IALLHIHTKGKDLVRPGMTRFATSYLTLGCLNDNKGSLIRMFLSDQW 387
>XP_016192700.1 PREDICTED: uncharacterized protein LOC107633607, partial [Arachis
ipaensis]
Length = 516
Score = 173 bits (438), Expect = 3e-49
Identities = 79/107 (73%), Positives = 94/107 (87%)
Frame = +1
Query: 61 SYKAAGDLLMQKRKKLYWTPCAAHCIELMLEDFEKKIPLHKETIASGKKITTYIYSRSGL 240
+YKAAG++LM+KRKKL+WTPCAAHCI+LMLED EKK+ LHK+TI G+KITTYIY+R+ L
Sbjct: 69 NYKAAGEMLMKKRKKLFWTPCAAHCIDLMLEDLEKKVTLHKDTIYKGRKITTYIYARTAL 128
Query: 241 VSLLHHFTKGKDLIRPAATRFATSYLTLGCLNDNKGALIRMFTSKAW 381
++LLH TKGKDL+R TRFATSYLTLGCLNDNKG+LIRMF S W
Sbjct: 129 IALLHIHTKGKDLVRLGMTRFATSYLTLGCLNDNKGSLIRMFFSDQW 175
>XP_019465412.1 PREDICTED: uncharacterized protein LOC109363616 [Lupinus
angustifolius]
Length = 459
Score = 171 bits (434), Expect = 4e-49
Identities = 84/108 (77%), Positives = 93/108 (86%)
Frame = +1
Query: 61 SYKAAGDLLMQKRKKLYWTPCAAHCIELMLEDFEKKIPLHKETIASGKKITTYIYSRSGL 240
+YKAAG +LM+KR +LYWTPCAAHCI+LMLEDFEK I +HKETI SGKKITTYIY R+ L
Sbjct: 278 NYKAAGQMLMEKRTRLYWTPCAAHCIDLMLEDFEK-IHVHKETIPSGKKITTYIYGRTFL 336
Query: 241 VSLLHHFTKGKDLIRPAATRFATSYLTLGCLNDNKGALIRMFTSKAWK 384
+SLL FTKGKDL+ PA TRFATSYLTLGCL DNKGAL RMFTS WK
Sbjct: 337 ISLLQKFTKGKDLVTPAMTRFATSYLTLGCLLDNKGALRRMFTSIEWK 384
>XP_011010809.1 PREDICTED: uncharacterized protein LOC105115580 [Populus
euphratica]
Length = 397
Score = 170 bits (430), Expect = 5e-49
Identities = 77/107 (71%), Positives = 95/107 (88%)
Frame = +1
Query: 61 SYKAAGDLLMQKRKKLYWTPCAAHCIELMLEDFEKKIPLHKETIASGKKITTYIYSRSGL 240
+YKAAG LLM+KRK L+WTPCAAHCI+L+LEDFEKK+ +H+ TIA G++IT+YIYSR+ L
Sbjct: 211 NYKAAGQLLMEKRKSLFWTPCAAHCIDLILEDFEKKLEIHQVTIAKGRRITSYIYSRTIL 270
Query: 241 VSLLHHFTKGKDLIRPAATRFATSYLTLGCLNDNKGALIRMFTSKAW 381
+S+L HFTKG+DLIRPAATRFAT+YLTLGCLND+K L+ MFTS W
Sbjct: 271 ISMLRHFTKGRDLIRPAATRFATAYLTLGCLNDHKIQLMTMFTSNQW 317
>XP_016195810.1 PREDICTED: uncharacterized protein LOC107636851 [Arachis ipaensis]
Length = 274
Score = 166 bits (420), Expect = 7e-49
Identities = 75/100 (75%), Positives = 88/100 (88%)
Frame = +1
Query: 82 LLMQKRKKLYWTPCAAHCIELMLEDFEKKIPLHKETIASGKKITTYIYSRSGLVSLLHHF 261
+LM+KRKKL+WTPCAAHCI+LMLED EKK+ LHK+TI G+KITTYIY+R+ L++LLH
Sbjct: 1 MLMKKRKKLFWTPCAAHCIDLMLEDLEKKVTLHKDTIYKGRKITTYIYARTALIALLHIH 60
Query: 262 TKGKDLIRPAATRFATSYLTLGCLNDNKGALIRMFTSKAW 381
TKGKDL+RP TRFATSYLTLGCLNDNKG+LIRMF S W
Sbjct: 61 TKGKDLVRPGMTRFATSYLTLGCLNDNKGSLIRMFLSDQW 100
>XP_011001694.1 PREDICTED: uncharacterized protein LOC105108893 [Populus
euphratica]
Length = 465
Score = 170 bits (430), Expect = 2e-48
Identities = 77/107 (71%), Positives = 95/107 (88%)
Frame = +1
Query: 61 SYKAAGDLLMQKRKKLYWTPCAAHCIELMLEDFEKKIPLHKETIASGKKITTYIYSRSGL 240
+YKAAG LLM+KRK L+WTPCAAHCI+L+LEDFEKK+ +H+ TIA G++IT+YIYSR+ L
Sbjct: 279 NYKAAGQLLMEKRKSLFWTPCAAHCIDLILEDFEKKLEIHQVTIAKGRRITSYIYSRTIL 338
Query: 241 VSLLHHFTKGKDLIRPAATRFATSYLTLGCLNDNKGALIRMFTSKAW 381
+S+L HFTKG+DLIRPAATRFAT+YLTLGCLND+K L+ MFTS W
Sbjct: 339 ISMLRHFTKGRDLIRPAATRFATAYLTLGCLNDHKIQLMTMFTSNQW 385
>GAU21484.1 hypothetical protein TSUD_242060 [Trifolium subterraneum]
Length = 749
Score = 174 bits (441), Expect = 2e-48
Identities = 81/108 (75%), Positives = 91/108 (84%)
Frame = +1
Query: 61 SYKAAGDLLMQKRKKLYWTPCAAHCIELMLEDFEKKIPLHKETIASGKKITTYIYSRSGL 240
+YKAAG LLM+KR LYWTPCAAHCI+LMLEDFEKKIP+HKETIA GKKITTYIY R+ L
Sbjct: 312 NYKAAGKLLMEKRSHLYWTPCAAHCIDLMLEDFEKKIPIHKETIAKGKKITTYIYGRTSL 371
Query: 241 VSLLHHFTKGKDLIRPAATRFATSYLTLGCLNDNKGALIRMFTSKAWK 384
+ +LH FTK DLIRP+ TRFATSYLTLGCLND++ LI MF S WK
Sbjct: 372 ICMLHKFTKNIDLIRPSLTRFATSYLTLGCLNDHQHELIEMFKSSEWK 419
>XP_007154637.1 hypothetical protein PHAVU_003G135300g [Phaseolus vulgaris]
ESW26631.1 hypothetical protein PHAVU_003G135300g
[Phaseolus vulgaris]
Length = 701
Score = 173 bits (438), Expect = 4e-48
Identities = 83/108 (76%), Positives = 95/108 (87%)
Frame = +1
Query: 61 SYKAAGDLLMQKRKKLYWTPCAAHCIELMLEDFEKKIPLHKETIASGKKITTYIYSRSGL 240
+YKAAGDLLMQKR+KLY TP AAHCI+LMLEDF KKIPLH+ETI++GKKIT YIYSR+ L
Sbjct: 292 NYKAAGDLLMQKRRKLYLTPSAAHCIDLMLEDFGKKIPLHQETISNGKKITAYIYSRTSL 351
Query: 241 VSLLHHFTKGKDLIRPAATRFATSYLTLGCLNDNKGALIRMFTSKAWK 384
+ LLH T+G DLIRP TRFATSYLTLGCLN+NK +LIRMFTSK W+
Sbjct: 352 ICLLHKHTEGIDLIRPVNTRFATSYLTLGCLNENKKSLIRMFTSKEWQ 399
>XP_007150061.1 hypothetical protein PHAVU_005G123000g, partial [Phaseolus
vulgaris] ESW22055.1 hypothetical protein
PHAVU_005G123000g, partial [Phaseolus vulgaris]
Length = 590
Score = 171 bits (433), Expect = 5e-48
Identities = 77/108 (71%), Positives = 93/108 (86%)
Frame = +1
Query: 61 SYKAAGDLLMQKRKKLYWTPCAAHCIELMLEDFEKKIPLHKETIASGKKITTYIYSRSGL 240
+YK AG +LM KRKKL+WTPCAAHC++LMLED+EKKI +H+ETI GKKITT+IYSR L
Sbjct: 209 NYKDAGQMLMTKRKKLFWTPCAAHCVDLMLEDYEKKISIHEETIPKGKKITTFIYSRPSL 268
Query: 241 VSLLHHFTKGKDLIRPAATRFATSYLTLGCLNDNKGALIRMFTSKAWK 384
+SLL HFTKG+ L+RP TRFATS+ TLGCL++NKGALI+MFTS WK
Sbjct: 269 ISLLQHFTKGRYLVRPDITRFATSFFTLGCLHENKGALIKMFTSDEWK 316