BLASTX nr result
ID: Glycyrrhiza35_contig00038060
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Glycyrrhiza35_contig00038060
(334 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
XP_007151521.1 hypothetical protein PHAVU_004G054000g [Phaseolus... 89 1e-18
XP_017437630.1 PREDICTED: cyclic dof factor 3-like isoform X3 [V... 86 1e-17
XP_017437628.1 PREDICTED: cyclic dof factor 1-like isoform X2 [V... 86 2e-17
XP_017437627.1 PREDICTED: cyclic dof factor 1-like isoform X1 [V... 86 2e-17
KRG94921.1 hypothetical protein GLYMA_19G118000 [Glycine max] 84 2e-16
NP_001304603.1 cyclic dof factor 3-like [Glycine max] ACU18311.1... 84 2e-16
DAA64920.1 TPA_inf: dof protein [Cajanus cajan] DAA64942.1 TPA_i... 83 3e-16
KRH64974.1 hypothetical protein GLYMA_03G007900 [Glycine max] 82 4e-16
KHN42188.1 Dof zinc finger protein DOF5.2 [Glycine soja] 82 4e-16
KHN34636.1 Dof zinc finger protein DOF5.2 [Glycine soja] 82 5e-16
KYP63909.1 Dof zinc finger protein DOF5.2 [Cajanus cajan] 82 6e-16
DAA64921.1 TPA_inf: dof protein [Cajanus cajan] 82 8e-16
GAU46700.1 hypothetical protein TSUD_140080 [Trifolium subterran... 76 3e-14
XP_019426775.1 PREDICTED: cyclic dof factor 3-like [Lupinus angu... 75 2e-13
XP_014504007.1 PREDICTED: cyclic dof factor 1-like isoform X2 [V... 71 3e-12
XP_014503999.1 PREDICTED: cyclic dof factor 1-like isoform X1 [V... 71 3e-12
XP_015946926.1 PREDICTED: cyclic dof factor 3-like [Arachis dura... 70 1e-11
XP_016179252.1 PREDICTED: cyclic dof factor 3-like [Arachis ipae... 67 7e-11
OAY41875.1 hypothetical protein MANES_09G136300 [Manihot esculenta] 67 1e-10
OAY41876.1 hypothetical protein MANES_09G136300 [Manihot esculenta] 67 1e-10
>XP_007151521.1 hypothetical protein PHAVU_004G054000g [Phaseolus vulgaris]
ESW23515.1 hypothetical protein PHAVU_004G054000g
[Phaseolus vulgaris]
Length = 450
Score = 89.4 bits (220), Expect = 1e-18
Identities = 62/121 (51%), Positives = 69/121 (57%), Gaps = 12/121 (9%)
Frame = +1
Query: 7 SSLHMFNNPYSSTGPASMQWCPAPPMMAVPTIFPPNNNTPLQFVPAGSYWHR-------- 162
SSLHM N PYS+ PASMQWCP P MMAVPTI+PP+ PLQFVP GSYW
Sbjct: 272 SSLHMCN-PYSAD-PASMQWCPTP-MMAVPTIYPPS--FPLQFVP-GSYWGGQPMWGGGT 325
Query: 163 -TVSIGSNGXXXXXXXXXXXXXXXXXXXXXXXKHTRDH---TDEERSEMCVFVPKTLRID 330
VS+G N KHTRD+ TDEE+S+MCV VP TLRID
Sbjct: 326 GAVSMGFNACLSPTSSTSNSCCSGNGSPTLG-KHTRDNNVSTDEEKSDMCVLVPTTLRID 384
Query: 331 D 333
D
Sbjct: 385 D 385
>XP_017437630.1 PREDICTED: cyclic dof factor 3-like isoform X3 [Vigna angularis]
XP_017437631.1 PREDICTED: cyclic dof factor 3-like
isoform X3 [Vigna angularis]
Length = 429
Score = 86.3 bits (212), Expect = 1e-17
Identities = 58/120 (48%), Positives = 66/120 (55%), Gaps = 11/120 (9%)
Frame = +1
Query: 7 SSLHMFNNPYSSTGPASMQWCPAPPMMAVPTIFPPNNNTPLQFVPAGSYWH--------- 159
SSL M N PY+S PASMQWCP P+MAVPTI PP + PLQFVP GSYW
Sbjct: 257 SSLQMCN-PYNSADPASMQWCPT-PIMAVPTICPP--SFPLQFVP-GSYWSGPPVYGGGT 311
Query: 160 RTVSIGSNGXXXXXXXXXXXXXXXXXXXXXXXKHTRDH--TDEERSEMCVFVPKTLRIDD 333
S+G N KHTRD+ TDEE+S+MC+ VP TLRIDD
Sbjct: 312 GAASMGFNA-CLSPTSSTSNSCCSGNGSPTLGKHTRDNLSTDEEKSDMCILVPTTLRIDD 370
>XP_017437628.1 PREDICTED: cyclic dof factor 1-like isoform X2 [Vigna angularis]
Length = 442
Score = 86.3 bits (212), Expect = 2e-17
Identities = 58/120 (48%), Positives = 66/120 (55%), Gaps = 11/120 (9%)
Frame = +1
Query: 7 SSLHMFNNPYSSTGPASMQWCPAPPMMAVPTIFPPNNNTPLQFVPAGSYWH--------- 159
SSL M N PY+S PASMQWCP P+MAVPTI PP + PLQFVP GSYW
Sbjct: 270 SSLQMCN-PYNSADPASMQWCPT-PIMAVPTICPP--SFPLQFVP-GSYWSGPPVYGGGT 324
Query: 160 RTVSIGSNGXXXXXXXXXXXXXXXXXXXXXXXKHTRDH--TDEERSEMCVFVPKTLRIDD 333
S+G N KHTRD+ TDEE+S+MC+ VP TLRIDD
Sbjct: 325 GAASMGFNA-CLSPTSSTSNSCCSGNGSPTLGKHTRDNLSTDEEKSDMCILVPTTLRIDD 383
>XP_017437627.1 PREDICTED: cyclic dof factor 1-like isoform X1 [Vigna angularis]
KOM56291.1 hypothetical protein LR48_Vigan10g218300
[Vigna angularis] BAU01511.1 hypothetical protein
VIGAN_11076000 [Vigna angularis var. angularis]
Length = 480
Score = 86.3 bits (212), Expect = 2e-17
Identities = 58/120 (48%), Positives = 66/120 (55%), Gaps = 11/120 (9%)
Frame = +1
Query: 7 SSLHMFNNPYSSTGPASMQWCPAPPMMAVPTIFPPNNNTPLQFVPAGSYWH--------- 159
SSL M N PY+S PASMQWCP P+MAVPTI PP + PLQFVP GSYW
Sbjct: 308 SSLQMCN-PYNSADPASMQWCPT-PIMAVPTICPP--SFPLQFVP-GSYWSGPPVYGGGT 362
Query: 160 RTVSIGSNGXXXXXXXXXXXXXXXXXXXXXXXKHTRDH--TDEERSEMCVFVPKTLRIDD 333
S+G N KHTRD+ TDEE+S+MC+ VP TLRIDD
Sbjct: 363 GAASMGFNA-CLSPTSSTSNSCCSGNGSPTLGKHTRDNLSTDEEKSDMCILVPTTLRIDD 421
>KRG94921.1 hypothetical protein GLYMA_19G118000 [Glycine max]
Length = 483
Score = 83.6 bits (205), Expect = 2e-16
Identities = 53/112 (47%), Positives = 62/112 (55%), Gaps = 11/112 (9%)
Frame = +1
Query: 28 NPYSSTGPASMQWCPAPPMMAVPTIFPPNNNTPLQFVPAGSYWHRT---------VSIGS 180
NPY++TGP +MQWCP PM+A+P + PP + PLQFVP SYW T VSIGS
Sbjct: 310 NPYNNTGPTAMQWCPT-PMVAIPGMCPP--SIPLQFVPP-SYWSGTPLWNAGTGAVSIGS 365
Query: 181 NGXXXXXXXXXXXXXXXXXXXXXXXKHTRDH--TDEERSEMCVFVPKTLRID 330
N KHTRD TDEE+SE CV VPKT+RID
Sbjct: 366 NA-CLSPTSSTNNSCCSGNGSPTLGKHTRDTVCTDEEKSEKCVLVPKTIRID 416
>NP_001304603.1 cyclic dof factor 3-like [Glycine max] ACU18311.1 unknown [Glycine
max]
Length = 483
Score = 83.6 bits (205), Expect = 2e-16
Identities = 53/112 (47%), Positives = 62/112 (55%), Gaps = 11/112 (9%)
Frame = +1
Query: 28 NPYSSTGPASMQWCPAPPMMAVPTIFPPNNNTPLQFVPAGSYWHRT---------VSIGS 180
NPY++TGP +MQWCP PM+A+P + PP + PLQFVP SYW T VSIGS
Sbjct: 310 NPYNNTGPTAMQWCPT-PMVAIPGMCPP--SIPLQFVPP-SYWSGTPLWNAGTGAVSIGS 365
Query: 181 NGXXXXXXXXXXXXXXXXXXXXXXXKHTRDH--TDEERSEMCVFVPKTLRID 330
N KHTRD TDEE+SE CV VPKT+RID
Sbjct: 366 NA-CLSPTSSTNNSCCSGNGSPTLGKHTRDTVCTDEEKSEKCVLVPKTIRID 416
>DAA64920.1 TPA_inf: dof protein [Cajanus cajan] DAA64942.1 TPA_inf: dof
protein [Cajanus cajan]
Length = 479
Score = 82.8 bits (203), Expect = 3e-16
Identities = 59/120 (49%), Positives = 66/120 (55%), Gaps = 11/120 (9%)
Frame = +1
Query: 7 SSLHMFNNPYSSTGPASMQWCPAPPMMAVPTIFPPNNNTPLQFVPAGSYWHR-------- 162
SSLHM + + S PASMQWCP P M+AV TI PP+ PLQFVP GSYW
Sbjct: 301 SSLHMCS--HYSADPASMQWCPTP-MVAVQTICPPS--FPLQFVP-GSYWGGPPVWGAGT 354
Query: 163 -TVSIGSNGXXXXXXXXXXXXXXXXXXXXXXXKHTRD--HTDEERSEMCVFVPKTLRIDD 333
+VSIG N KHTRD TDEE+S+MCV VPKTLRIDD
Sbjct: 355 GSVSIGFNACLSPTSSTSNSCCSGNGSPTLG-KHTRDTVFTDEEKSDMCVLVPKTLRIDD 413
>KRH64974.1 hypothetical protein GLYMA_03G007900 [Glycine max]
Length = 461
Score = 82.4 bits (202), Expect = 4e-16
Identities = 53/112 (47%), Positives = 63/112 (56%), Gaps = 11/112 (9%)
Frame = +1
Query: 28 NPYSSTGPASMQWCPAPPMMAVPTIFPPNNNTPLQFVPAGSYWH---------RTVSIGS 180
NPY++TGPA+MQWCP PM+A+P + PP + PLQFVP SYW TVSIGS
Sbjct: 289 NPYNNTGPAAMQWCPT-PMVAIPGMCPP--SIPLQFVPP-SYWSGTQLWNAGTGTVSIGS 344
Query: 181 NGXXXXXXXXXXXXXXXXXXXXXXXKHTRDH--TDEERSEMCVFVPKTLRID 330
N KHTRD T+EE+SE CV VPKT+RID
Sbjct: 345 NA-CLSPSSSTSNSCCSGNGSPTLGKHTRDTVCTNEEKSEKCVSVPKTIRID 395
>KHN42188.1 Dof zinc finger protein DOF5.2 [Glycine soja]
Length = 482
Score = 82.4 bits (202), Expect = 4e-16
Identities = 53/112 (47%), Positives = 63/112 (56%), Gaps = 11/112 (9%)
Frame = +1
Query: 28 NPYSSTGPASMQWCPAPPMMAVPTIFPPNNNTPLQFVPAGSYWH---------RTVSIGS 180
NPY++TGPA+MQWCP PM+A+P + PP + PLQFVP SYW TVSIGS
Sbjct: 310 NPYNNTGPAAMQWCPT-PMVAIPGMCPP--SIPLQFVPP-SYWSGTQLWNAGTGTVSIGS 365
Query: 181 NGXXXXXXXXXXXXXXXXXXXXXXXKHTRDH--TDEERSEMCVFVPKTLRID 330
N KHTRD T+EE+SE CV VPKT+RID
Sbjct: 366 NA-CLSPSSSTSNSCCSGNGSPTLGKHTRDTVCTNEEKSEKCVSVPKTIRID 416
>KHN34636.1 Dof zinc finger protein DOF5.2 [Glycine soja]
Length = 483
Score = 82.0 bits (201), Expect = 5e-16
Identities = 52/112 (46%), Positives = 62/112 (55%), Gaps = 11/112 (9%)
Frame = +1
Query: 28 NPYSSTGPASMQWCPAPPMMAVPTIFPPNNNTPLQFVPAGSYWHRT---------VSIGS 180
NPY++TGP +MQWCP PM+A+P + PP + PLQFVP SYW T VSIGS
Sbjct: 310 NPYNNTGPTAMQWCPT-PMVAIPGMCPP--SIPLQFVPP-SYWSGTPLWNAGTGAVSIGS 365
Query: 181 NGXXXXXXXXXXXXXXXXXXXXXXXKHTRDH--TDEERSEMCVFVPKTLRID 330
N KHTRD TDEE+SE CV VPKT+RI+
Sbjct: 366 NA-CLSPTSSTNNSCCSGNGSPTLGKHTRDTVCTDEEKSEKCVLVPKTIRIE 416
>KYP63909.1 Dof zinc finger protein DOF5.2 [Cajanus cajan]
Length = 415
Score = 81.6 bits (200), Expect = 6e-16
Identities = 57/122 (46%), Positives = 65/122 (53%), Gaps = 13/122 (10%)
Frame = +1
Query: 4 QSSLHMFNNPYSSTGPASMQWCPAPPMMAVPTIFPPNNNTPLQFVPAGSYWHRT------ 165
QSS M N+PY+ TGPASMQWCP P M+A+P + PP+ PLQ VP SYW T
Sbjct: 234 QSSAPMCNSPYN-TGPASMQWCPTP-MVAIPGMCPPS--IPLQLVPP-SYWGGTPLWNGG 288
Query: 166 ---VSIGSNGXXXXXXXXXXXXXXXXXXXXXXXKHTRDH----TDEERSEMCVFVPKTLR 324
VSIGSN KHTRD DEE+SE CV VPKT+R
Sbjct: 289 TGAVSIGSNACLSPSSSTSNSCCSGNVSPTLG-KHTRDKDNAFADEEKSEKCVLVPKTIR 347
Query: 325 ID 330
ID
Sbjct: 348 ID 349
>DAA64921.1 TPA_inf: dof protein [Cajanus cajan]
Length = 540
Score = 81.6 bits (200), Expect = 8e-16
Identities = 57/122 (46%), Positives = 65/122 (53%), Gaps = 13/122 (10%)
Frame = +1
Query: 4 QSSLHMFNNPYSSTGPASMQWCPAPPMMAVPTIFPPNNNTPLQFVPAGSYWHRT------ 165
QSS M N+PY+ TGPASMQWCP P M+A+P + PP+ PLQ VP SYW T
Sbjct: 359 QSSAPMCNSPYN-TGPASMQWCPTP-MVAIPGMCPPS--IPLQLVPP-SYWGGTPLWNGG 413
Query: 166 ---VSIGSNGXXXXXXXXXXXXXXXXXXXXXXXKHTRDH----TDEERSEMCVFVPKTLR 324
VSIGSN KHTRD DEE+SE CV VPKT+R
Sbjct: 414 TGAVSIGSNACLSPSSSTSNSCCSGNVSPTLG-KHTRDKDNAFADEEKSEKCVLVPKTIR 472
Query: 325 ID 330
ID
Sbjct: 473 ID 474
>GAU46700.1 hypothetical protein TSUD_140080 [Trifolium subterraneum]
Length = 312
Score = 76.3 bits (186), Expect = 3e-14
Identities = 53/113 (46%), Positives = 61/113 (53%), Gaps = 2/113 (1%)
Frame = +1
Query: 1 VQSSLHMFNNPYSSTGPASMQWCPAPPMMAVPTIFPPNNNTPLQFVPAGSYWHRTVSIGS 180
VQSS+ M+ P +MQWC AP M+AV IF PN LQFVP GSY + VSIGS
Sbjct: 210 VQSSMQMY--------PTTMQWCHAP-MVAVTNIFTPNIG--LQFVP-GSYGNGNVSIGS 257
Query: 181 NGXXXXXXXXXXXXXXXXXXXXXXXKHTRDH--TDEERSEMCVFVPKTLRIDD 333
NG KHTRD TDEE+ EMC+F P TLRID+
Sbjct: 258 NGCISPSSSTTSNSLGNGSPILG--KHTRDSVLTDEEKPEMCLFAPNTLRIDE 308
>XP_019426775.1 PREDICTED: cyclic dof factor 3-like [Lupinus angustifolius]
OIV90088.1 hypothetical protein TanjilG_01542 [Lupinus
angustifolius]
Length = 420
Score = 74.7 bits (182), Expect = 2e-13
Identities = 53/121 (43%), Positives = 63/121 (52%), Gaps = 11/121 (9%)
Frame = +1
Query: 4 QSSLHMFNNPYSSTGPASMQWCPAPPMMAVPTIFPPNNNTPLQFVPAGSYWHRTV----- 168
Q SL M N SMQWC AP M+AVPT+ PP+ PLQFVPA SYW T
Sbjct: 306 QPSLRMCNPS------VSMQWCSAP-MVAVPTMCPPS--MPLQFVPA-SYWSGTPVWTTA 355
Query: 169 ----SIGSNGXXXXXXXXXXXXXXXXXXXXXXXKHTRDH--TDEERSEMCVFVPKTLRID 330
+IGSNG KHTRD +DEE+S+MCVFVPK+LR +
Sbjct: 356 AGTGAIGSNGYLSPSSSTSNNSCCSGNGSPILGKHTRDTVLSDEEKSDMCVFVPKSLRTE 415
Query: 331 D 333
+
Sbjct: 416 E 416
>XP_014504007.1 PREDICTED: cyclic dof factor 1-like isoform X2 [Vigna radiata var.
radiata]
Length = 427
Score = 71.2 bits (173), Expect = 3e-12
Identities = 51/110 (46%), Positives = 58/110 (52%), Gaps = 11/110 (10%)
Frame = +1
Query: 7 SSLHMFNNPYSSTGPASMQWCPAPPMMAVPTIFPPNNNTPLQFVPAGSYWH--------- 159
SSL M N PY+S PASMQWCP P+MAVPTI PP + PLQFVP GSYW
Sbjct: 271 SSLQMCN-PYNSADPASMQWCPT-PIMAVPTICPP--SFPLQFVP-GSYWSGPPVYGGGT 325
Query: 160 RTVSIGSNGXXXXXXXXXXXXXXXXXXXXXXXKHTRDH--TDEERSEMCV 303
S+G N KHTRD+ TDEE+S+MCV
Sbjct: 326 GAASMGFNA-CLSPTSSTSNSCCSGNGSPTLGKHTRDNLSTDEEKSDMCV 374
>XP_014503999.1 PREDICTED: cyclic dof factor 1-like isoform X1 [Vigna radiata var.
radiata]
Length = 465
Score = 71.2 bits (173), Expect = 3e-12
Identities = 51/110 (46%), Positives = 58/110 (52%), Gaps = 11/110 (10%)
Frame = +1
Query: 7 SSLHMFNNPYSSTGPASMQWCPAPPMMAVPTIFPPNNNTPLQFVPAGSYWH--------- 159
SSL M N PY+S PASMQWCP P+MAVPTI PP + PLQFVP GSYW
Sbjct: 309 SSLQMCN-PYNSADPASMQWCPT-PIMAVPTICPP--SFPLQFVP-GSYWSGPPVYGGGT 363
Query: 160 RTVSIGSNGXXXXXXXXXXXXXXXXXXXXXXXKHTRDH--TDEERSEMCV 303
S+G N KHTRD+ TDEE+S+MCV
Sbjct: 364 GAASMGFNA-CLSPTSSTSNSCCSGNGSPTLGKHTRDNLSTDEEKSDMCV 412
>XP_015946926.1 PREDICTED: cyclic dof factor 3-like [Arachis duranensis]
Length = 412
Score = 69.7 bits (169), Expect = 1e-11
Identities = 48/113 (42%), Positives = 56/113 (49%), Gaps = 12/113 (10%)
Frame = +1
Query: 28 NPYSSTGPASMQWCPAPPMMAVPTIFPPNNNTPLQFVPAGSYWHRT---------VSIGS 180
NP + G + + M VPTI PPN PLQFVP GS+W+RT VSIGS
Sbjct: 247 NPGWNNGGSPVPLTFPATMRGVPTISPPN--IPLQFVP-GSFWNRTQVWPSQTAAVSIGS 303
Query: 181 NGXXXXXXXXXXXXXXXXXXXXXXXKHTRDHT---DEERSEMCVFVPKTLRID 330
NG KHTRD D+E+SE+CV VPKTLRID
Sbjct: 304 NGCLSPSSSTTSNSCCSGNGSPTLGKHTRDSNVFIDDEKSEICVLVPKTLRID 356
>XP_016179252.1 PREDICTED: cyclic dof factor 3-like [Arachis ipaensis]
Length = 414
Score = 67.4 bits (163), Expect = 7e-11
Identities = 47/113 (41%), Positives = 55/113 (48%), Gaps = 12/113 (10%)
Frame = +1
Query: 28 NPYSSTGPASMQWCPAPPMMAVPTIFPPNNNTPLQFVPAGSYWHRT---------VSIGS 180
NP + G + + M VPTI PPN PLQFVP GS+W+R VSIGS
Sbjct: 249 NPGWNNGGSPVPLTFPATMRGVPTISPPN--IPLQFVP-GSFWNRAQVWPLQTAAVSIGS 305
Query: 181 NGXXXXXXXXXXXXXXXXXXXXXXXKHTRDHT---DEERSEMCVFVPKTLRID 330
NG KHTRD D+E+SE+CV VPKTLRID
Sbjct: 306 NGCLSPSSSTTSNSCCSGNGSPTLGKHTRDGNVFMDDEKSEICVLVPKTLRID 358
>OAY41875.1 hypothetical protein MANES_09G136300 [Manihot esculenta]
Length = 355
Score = 66.6 bits (161), Expect = 1e-10
Identities = 42/110 (38%), Positives = 53/110 (48%), Gaps = 11/110 (10%)
Frame = +1
Query: 37 SSTGPASMQWCPAPPMMAVPTIFPPNNNTPLQFVPAGSYWH---------RTVSIGSNGX 189
+S + +QWCP P ++A+P PPN PLQFVPA SYW R S+G +
Sbjct: 183 NSISSSQVQWCPTP-VLAIPGFCPPN--VPLQFVPA-SYWGCMPVWDAGTRNTSLGESNG 238
Query: 190 XXXXXXXXXXXXXXXXXXXXXXKHTRDHT--DEERSEMCVFVPKTLRIDD 333
KH+RD DEE+ E C+ VPKTLRIDD
Sbjct: 239 CSSPSSSTTTSCCSGNGSPNLGKHSRDSNLMDEEKPENCILVPKTLRIDD 288
>OAY41876.1 hypothetical protein MANES_09G136300 [Manihot esculenta]
Length = 500
Score = 66.6 bits (161), Expect = 1e-10
Identities = 42/110 (38%), Positives = 53/110 (48%), Gaps = 11/110 (10%)
Frame = +1
Query: 37 SSTGPASMQWCPAPPMMAVPTIFPPNNNTPLQFVPAGSYWH---------RTVSIGSNGX 189
+S + +QWCP P ++A+P PPN PLQFVPA SYW R S+G +
Sbjct: 328 NSISSSQVQWCPTP-VLAIPGFCPPN--VPLQFVPA-SYWGCMPVWDAGTRNTSLGESNG 383
Query: 190 XXXXXXXXXXXXXXXXXXXXXXKHTRDHT--DEERSEMCVFVPKTLRIDD 333
KH+RD DEE+ E C+ VPKTLRIDD
Sbjct: 384 CSSPSSSTTTSCCSGNGSPNLGKHSRDSNLMDEEKPENCILVPKTLRIDD 433