BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Glycyrrhiza31_contig00010834
         (1377 letters)

Database: ./nr 
           115,041,592 sequences; 42,171,959,267 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_003588760.1 IDS4-like protein [Medicago truncatula] AES59011....   470   e-163
XP_012570720.1 PREDICTED: SPX domain-containing protein 2-like [...   466   e-161
GAU15099.1 hypothetical protein TSUD_08370 [Trifolium subterraneum]   462   e-160
AFK42649.1 unknown [Lotus japonicus]                                  447   e-154
XP_019447544.1 PREDICTED: SPX domain-containing protein 2-like [...   418   e-142
XP_016162695.1 PREDICTED: SPX domain-containing protein 2-like [...   417   e-142
XP_015971967.1 PREDICTED: SPX domain-containing protein 2-like [...   417   e-141
XP_019438769.1 PREDICTED: SPX domain-containing protein 2-like [...   396   e-134
OIW09278.1 hypothetical protein TanjilG_01249 [Lupinus angustifo...   396   e-133
KYP43409.1 Xenotropic and polytropic retrovirus receptor 1 isoge...   357   e-119
XP_014629815.1 PREDICTED: uncharacterized protein LOC100499977 i...   357   e-119
NP_001235083.1 uncharacterized protein LOC100499977 [Glycine max...   356   e-118
KRH52450.1 hypothetical protein GLYMA_06G069000 [Glycine max]         355   e-118
KHN07083.1 SPX domain-containing protein 1 [Glycine soja]             350   e-116
XP_009355732.1 PREDICTED: SPX domain-containing protein 1-like [...   339   e-111
XP_008394070.1 PREDICTED: SPX domain-containing protein 1-like [...   335   e-110
XP_007011873.2 PREDICTED: SPX domain-containing protein 2 [Theob...   333   e-109
XP_018822225.1 PREDICTED: SPX domain-containing protein 1-like [...   332   e-109
EOY29492.1 SPX domain-containing protein 2 [Theobroma cacao]          332   e-108
OAY27376.1 hypothetical protein MANES_16G121300 [Manihot esculenta]   332   e-108

>XP_003588760.1 IDS4-like protein [Medicago truncatula] AES59011.1 IDS4-like protein
            [Medicago truncatula]
          Length = 274

 Score =  470 bits (1209), Expect = e-163
 Identities = 239/274 (87%), Positives = 254/274 (92%), Gaps = 5/274 (1%)
 Frame = -2

Query: 1235 MKFWKILSNQIEQTLPDWRDKFLSYKDLKKQLKLIAPN--DSSSNKRRRFDDEGG---EV 1071
            MKFWKIL+NQIEQTLPDWRDKFLSYKDLKKQLKLI P   DSS +KRRR DD+GG   EV
Sbjct: 1    MKFWKILNNQIEQTLPDWRDKFLSYKDLKKQLKLIVPKEIDSSCSKRRRLDDDGGAEGEV 60

Query: 1070 TKEVNDFLKLLEVEIEKFNAFFVEKEEEYVITWKELQDKVAWAKNSDVELMSVGREIVDF 891
            TKEV DFL+LLEVEIEKFN FFVEKEEEYVI WKELQDKVAWAK+SD+ELM+VGREIVDF
Sbjct: 61   TKEVKDFLRLLEVEIEKFNGFFVEKEEEYVIKWKELQDKVAWAKSSDIELMTVGREIVDF 120

Query: 890  HGEMVLLENYSALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLNKLVKECE 711
            HGEMVLLENYSALNYTGLVKIIKKYDKRTGAL+RLPFIQDVLNQPFFKIDVLNKLVKECE
Sbjct: 121  HGEMVLLENYSALNYTGLVKIIKKYDKRTGALLRLPFIQDVLNQPFFKIDVLNKLVKECE 180

Query: 710  VMLSILFPKSGPLGPSLSTSEVFEEDACGSVTANENKEALEQVPRDFAEIQNMENVFIKL 531
            VMLSI+FPKSGPLG SLSTSEVFEE A  + TANE KE L+ VP++F+EIQNMEN+FIKL
Sbjct: 181  VMLSIIFPKSGPLGQSLSTSEVFEEVARETTTANETKETLDHVPKEFSEIQNMENIFIKL 240

Query: 530  TTSALDTLKEIRGGSSTVSIYSLPPLHSETLVED 429
            TTSALDTLKEIRGGSSTVSIYSLPPLHSETLVED
Sbjct: 241  TTSALDTLKEIRGGSSTVSIYSLPPLHSETLVED 274


>XP_012570720.1 PREDICTED: SPX domain-containing protein 2-like [Cicer arietinum]
          Length = 272

 Score =  466 bits (1199), Expect = e-161
 Identities = 237/272 (87%), Positives = 249/272 (91%), Gaps = 3/272 (1%)
 Frame = -2

Query: 1235 MKFWKILSNQIEQTLPDWRDKFLSYKDLKKQLKLIAPNDSSSNKRRRFDDEGG---EVTK 1065
            MKFWKILSNQIEQTLPDWRDKFLSYKDLKKQLKLI P D SS KRRR D++G    +V+K
Sbjct: 1    MKFWKILSNQIEQTLPDWRDKFLSYKDLKKQLKLINPKDPSSFKRRRLDEDGNADADVSK 60

Query: 1064 EVNDFLKLLEVEIEKFNAFFVEKEEEYVITWKELQDKVAWAKNSDVELMSVGREIVDFHG 885
            EVNDFL+LLEVEIEKFNAFFVE EEEYVI WKELQDKVAWAKNSD ELMSVGREIVDFHG
Sbjct: 61   EVNDFLRLLEVEIEKFNAFFVEMEEEYVIKWKELQDKVAWAKNSDAELMSVGREIVDFHG 120

Query: 884  EMVLLENYSALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLNKLVKECEVM 705
            EMVLLENYSALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLNKLVKECE+M
Sbjct: 121  EMVLLENYSALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLNKLVKECELM 180

Query: 704  LSILFPKSGPLGPSLSTSEVFEEDACGSVTANENKEALEQVPRDFAEIQNMENVFIKLTT 525
            LSI+FPKSGPLG SLSTSEVFEE   GS +AN  KE  E VP++ +EIQNMEN+FIKLTT
Sbjct: 181  LSIIFPKSGPLGQSLSTSEVFEEVTRGSTSANGTKETFEHVPKELSEIQNMENIFIKLTT 240

Query: 524  SALDTLKEIRGGSSTVSIYSLPPLHSETLVED 429
            SALDTLKEIRGGSSTVSIYSLPPLHSETLVED
Sbjct: 241  SALDTLKEIRGGSSTVSIYSLPPLHSETLVED 272


>GAU15099.1 hypothetical protein TSUD_08370 [Trifolium subterraneum]
          Length = 279

 Score =  462 bits (1188), Expect = e-160
 Identities = 238/279 (85%), Positives = 254/279 (91%), Gaps = 10/279 (3%)
 Frame = -2

Query: 1235 MKFWKILSNQIEQTLPDWRDKFLSYKDLKKQLKLIAPNDSSSN-KRRRFDDEG-----GE 1074
            MKFWKIL+NQIEQTLPDWRDKFLSYKDLKKQLKLI P DSSS+ KRRR DD+G     GE
Sbjct: 1    MKFWKILNNQIEQTLPDWRDKFLSYKDLKKQLKLIDPKDSSSSSKRRRLDDDGATNADGE 60

Query: 1073 VTKEVNDFLKLLEVEIEKFNAFFVEKEEEYVITWKELQDKVAWAKNSDVELMSVGREIVD 894
            VTKEVNDFLKLLE EIEKFN FFVEKEEEYVI WKELQDKVAWAKNSD+ELM+VGREIVD
Sbjct: 61   VTKEVNDFLKLLEDEIEKFNGFFVEKEEEYVIKWKELQDKVAWAKNSDLELMTVGREIVD 120

Query: 893  FHGEMVLLENYSALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLNKLVKEC 714
            FHGEMVLLENYSALNYTGLVKIIKKYDKRTGAL+RLPFIQDVLNQPFFKIDVLNKLVKEC
Sbjct: 121  FHGEMVLLENYSALNYTGLVKIIKKYDKRTGALLRLPFIQDVLNQPFFKIDVLNKLVKEC 180

Query: 713  EVMLSILFPKSGPLGPSLSTSEVFEEDACGSVTANENKEALEQVPRDFAEIQNMENVFIK 534
            EVMLSI+FPKSGPLG SLSTS+VF+E A GS + +E KE LE VP++ +EIQNMEN+FIK
Sbjct: 181  EVMLSIIFPKSGPLGQSLSTSDVFDEVARGSTSTDETKETLEHVPKELSEIQNMENIFIK 240

Query: 533  LTTSALDTLKEIRGGSSTVSIYSLPPLH----SETLVED 429
            LTTSALDTLKEIRGGSSTVSIYSLPP+H    SETLVED
Sbjct: 241  LTTSALDTLKEIRGGSSTVSIYSLPPMHSGPNSETLVED 279


>AFK42649.1 unknown [Lotus japonicus]
          Length = 276

 Score =  447 bits (1151), Expect = e-154
 Identities = 233/274 (85%), Positives = 250/274 (91%), Gaps = 6/274 (2%)
 Frame = -2

Query: 1235 MKFWKILSNQIEQTLPDWRDKFLSYKDLKKQLKLIAPND----SSSNKRRRFD--DEGGE 1074
            MKFWKILSNQIEQTLPDWRDKFLSYKDLKKQLKLIAP +    SSS KRRR D  D  GE
Sbjct: 1    MKFWKILSNQIEQTLPDWRDKFLSYKDLKKQLKLIAPKEPSSSSSSLKRRRSDNDDGAGE 60

Query: 1073 VTKEVNDFLKLLEVEIEKFNAFFVEKEEEYVITWKELQDKVAWAKNSDVELMSVGREIVD 894
            V+KEVNDFL+LLEVEIEKFNAFFVE EEEYVI WKELQ+KVAWAKNSDV+LM VGREIVD
Sbjct: 61   VSKEVNDFLRLLEVEIEKFNAFFVEMEEEYVIKWKELQNKVAWAKNSDVDLMPVGREIVD 120

Query: 893  FHGEMVLLENYSALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLNKLVKEC 714
             HGEMVLLENYSALNYTGLVKIIKKYDKRTGAL+RLPFIQDVLNQPFFKIDVLNKLVKEC
Sbjct: 121  LHGEMVLLENYSALNYTGLVKIIKKYDKRTGALLRLPFIQDVLNQPFFKIDVLNKLVKEC 180

Query: 713  EVMLSILFPKSGPLGPSLSTSEVFEEDACGSVTANENKEALEQVPRDFAEIQNMENVFIK 534
            EVMLSILFPKSG LGPS STS++ EE+AC S+TANEN+E L+QVP++ AEIQNME++FIK
Sbjct: 181  EVMLSILFPKSGSLGPSFSTSDL-EEEACSSMTANENRETLKQVPKELAEIQNMESMFIK 239

Query: 533  LTTSALDTLKEIRGGSSTVSIYSLPPLHSETLVE 432
            LTTSALDTLKEIRGGSSTVSIYSLPP H E +VE
Sbjct: 240  LTTSALDTLKEIRGGSSTVSIYSLPP-HKEAMVE 272


>XP_019447544.1 PREDICTED: SPX domain-containing protein 2-like [Lupinus
            angustifolius]
          Length = 276

 Score =  418 bits (1074), Expect = e-142
 Identities = 214/275 (77%), Positives = 241/275 (87%), Gaps = 7/275 (2%)
 Frame = -2

Query: 1235 MKFWKILSNQIEQTLPDWRDKFLSYKDLKKQLKLIAPNDSS--SNKRRRFDDEG-----G 1077
            MKFWKILSNQIEQTLP+WRDKFLSYKDLKK LKLI+PN     S KR RFD +G     G
Sbjct: 1    MKFWKILSNQIEQTLPEWRDKFLSYKDLKKHLKLISPNHLHYPSPKRIRFDRDGDGDGDG 60

Query: 1076 EVTKEVNDFLKLLEVEIEKFNAFFVEKEEEYVITWKELQDKVAWAKNSDVELMSVGREIV 897
            +V+KEV DFL+LLEVEI+KFNAFFVE+EEEY+I WKELQD+VA AK+S++ELMSVGREIV
Sbjct: 61   DVSKEVKDFLRLLEVEIDKFNAFFVEQEEEYIIKWKELQDRVAVAKDSNMELMSVGREIV 120

Query: 896  DFHGEMVLLENYSALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLNKLVKE 717
            DFHGEMVLLENYSALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLNKLVKE
Sbjct: 121  DFHGEMVLLENYSALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLNKLVKE 180

Query: 716  CEVMLSILFPKSGPLGPSLSTSEVFEEDACGSVTANENKEALEQVPRDFAEIQNMENVFI 537
            CEVMLSILFPK+  L PSLS SE FEED C + T NENKE L  VP++ +EI+NMEN+F+
Sbjct: 181  CEVMLSILFPKNRSLVPSLSISEDFEEDGCTTTTVNENKETLVHVPKELSEIENMENMFM 240

Query: 536  KLTTSALDTLKEIRGGSSTVSIYSLPPLHSETLVE 432
            KLT SAL TL++IRGGSSTVS+YSLPP+H++ L E
Sbjct: 241  KLTQSALQTLEQIRGGSSTVSMYSLPPMHNKALEE 275


>XP_016162695.1 PREDICTED: SPX domain-containing protein 2-like [Arachis ipaensis]
          Length = 284

 Score =  417 bits (1071), Expect = e-142
 Identities = 212/280 (75%), Positives = 241/280 (86%), Gaps = 11/280 (3%)
 Frame = -2

Query: 1235 MKFWKILSNQIEQTLPDWRDKFLSYKDLKKQLKLIAPNDSSSN----KRRRFDDEGG--- 1077
            MKFWKIL+NQIEQTLPDWRDKFLSYKDLKKQLKLIAP   + N    KR R D +G    
Sbjct: 1    MKFWKILNNQIEQTLPDWRDKFLSYKDLKKQLKLIAPAKDADNNPPTKRPRLDGDGAAED 60

Query: 1076 ----EVTKEVNDFLKLLEVEIEKFNAFFVEKEEEYVITWKELQDKVAWAKNSDVELMSVG 909
                EV KEVNDFLKLLE+EIEKFNAFFVEKEEEY+I WKELQD+VA AK+S+VELMSVG
Sbjct: 61   GEQEEVAKEVNDFLKLLELEIEKFNAFFVEKEEEYIIKWKELQDRVAKAKDSNVELMSVG 120

Query: 908  REIVDFHGEMVLLENYSALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLNK 729
            REIVDFHGEMVLLENYSALNYTGLVKIIKKYDKRTGAL+RLPFIQDVLNQPFFK+DVLNK
Sbjct: 121  REIVDFHGEMVLLENYSALNYTGLVKIIKKYDKRTGALVRLPFIQDVLNQPFFKVDVLNK 180

Query: 728  LVKECEVMLSILFPKSGPLGPSLSTSEVFEEDACGSVTANENKEALEQVPRDFAEIQNME 549
            +VKECEVMLSILFP++ P G SLS ++ + E+ C S+T NE+K +L QVP++ AEI+NME
Sbjct: 181  IVKECEVMLSILFPRNRPPGSSLSINDDYTEEGCASITTNESKASLAQVPKELAEIENME 240

Query: 548  NVFIKLTTSALDTLKEIRGGSSTVSIYSLPPLHSETLVED 429
            N +IKLT+SAL TL++IRGGSSTVSIYSLPPL S+ L E+
Sbjct: 241  NTYIKLTSSALQTLEQIRGGSSTVSIYSLPPLGSKALEEE 280


>XP_015971967.1 PREDICTED: SPX domain-containing protein 2-like [Arachis duranensis]
          Length = 370

 Score =  417 bits (1072), Expect = e-141
 Identities = 213/280 (76%), Positives = 241/280 (86%), Gaps = 11/280 (3%)
 Frame = -2

Query: 1235 MKFWKILSNQIEQTLPDWRDKFLSYKDLKKQLKLIAPNDSSSN----KRRRFDDEGG--- 1077
            MKFWKIL+NQIEQTLPDWRDKFLSYKDLKKQLKLIAP   + N    KR R D +G    
Sbjct: 87   MKFWKILNNQIEQTLPDWRDKFLSYKDLKKQLKLIAPAKDADNNPPTKRPRLDGDGAAED 146

Query: 1076 ----EVTKEVNDFLKLLEVEIEKFNAFFVEKEEEYVITWKELQDKVAWAKNSDVELMSVG 909
                EV KEVNDFLKLLE+EIEKFNAFFVEKEEEY+I WKELQD+VA AK+S+VELMSVG
Sbjct: 147  GEQEEVAKEVNDFLKLLELEIEKFNAFFVEKEEEYIIKWKELQDRVAKAKDSNVELMSVG 206

Query: 908  REIVDFHGEMVLLENYSALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLNK 729
            REIVDFHGEMVLLENYSALNYTGLVKIIKKYDKRTGAL+RLPFIQDVLNQPFFK+DVLNK
Sbjct: 207  REIVDFHGEMVLLENYSALNYTGLVKIIKKYDKRTGALVRLPFIQDVLNQPFFKVDVLNK 266

Query: 728  LVKECEVMLSILFPKSGPLGPSLSTSEVFEEDACGSVTANENKEALEQVPRDFAEIQNME 549
            +VKECEVMLSILFP++ P G SLS +E + E+ C S+T NE+K +L QVP++ AEI+NME
Sbjct: 267  IVKECEVMLSILFPRNRPPGSSLSINEDYTEEGCTSITTNESKASLAQVPKELAEIENME 326

Query: 548  NVFIKLTTSALDTLKEIRGGSSTVSIYSLPPLHSETLVED 429
            N +IKLT+SAL TL++IRGGSSTVSIYSLPPL S+ L E+
Sbjct: 327  NTYIKLTSSALQTLEQIRGGSSTVSIYSLPPLGSKALEEE 366


>XP_019438769.1 PREDICTED: SPX domain-containing protein 2-like [Lupinus
            angustifolius] OIW14394.1 hypothetical protein
            TanjilG_15748 [Lupinus angustifolius]
          Length = 258

 Score =  396 bits (1018), Expect = e-134
 Identities = 201/268 (75%), Positives = 235/268 (87%)
 Frame = -2

Query: 1235 MKFWKILSNQIEQTLPDWRDKFLSYKDLKKQLKLIAPNDSSSNKRRRFDDEGGEVTKEVN 1056
            MKFWKILSNQIEQTLP+WRDKFLSYKDLKKQLKLI P+ SS            +V+++V 
Sbjct: 1    MKFWKILSNQIEQTLPEWRDKFLSYKDLKKQLKLIDPHHSSL-----------QVSQQVK 49

Query: 1055 DFLKLLEVEIEKFNAFFVEKEEEYVITWKELQDKVAWAKNSDVELMSVGREIVDFHGEMV 876
            DFLKLLE+EI+KFN+FFV+KEEEY+I  KELQ++VA AK+S++ELMS+ REIVDFHGEMV
Sbjct: 50   DFLKLLELEIDKFNSFFVDKEEEYIIKLKELQERVADAKDSNMELMSIWREIVDFHGEMV 109

Query: 875  LLENYSALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLNKLVKECEVMLSI 696
            LLENY+ALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLNKLVKECEVMLSI
Sbjct: 110  LLENYTALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLNKLVKECEVMLSI 169

Query: 695  LFPKSGPLGPSLSTSEVFEEDACGSVTANENKEALEQVPRDFAEIQNMENVFIKLTTSAL 516
            LFPK+  L PSLS S+ +E+  CGS+TA+ENKE L QVP++ AEI++MEN+FIKLT SAL
Sbjct: 170  LFPKNRSLAPSLSISKFYEDGGCGSITADENKEILVQVPKELAEIESMENMFIKLTQSAL 229

Query: 515  DTLKEIRGGSSTVSIYSLPPLHSETLVE 432
             TL++IRGGSSTVS+YSLPPLH++ L E
Sbjct: 230  QTLEQIRGGSSTVSMYSLPPLHNKALEE 257


>OIW09278.1 hypothetical protein TanjilG_01249 [Lupinus angustifolius]
          Length = 333

 Score =  396 bits (1017), Expect = e-133
 Identities = 204/259 (78%), Positives = 227/259 (87%), Gaps = 7/259 (2%)
 Frame = -2

Query: 1235 MKFWKILSNQIEQTLPDWRDKFLSYKDLKKQLKLIAPNDSS--SNKRRRFDDEG-----G 1077
            MKFWKILSNQIEQTLP+WRDKFLSYKDLKK LKLI+PN     S KR RFD +G     G
Sbjct: 1    MKFWKILSNQIEQTLPEWRDKFLSYKDLKKHLKLISPNHLHYPSPKRIRFDRDGDGDGDG 60

Query: 1076 EVTKEVNDFLKLLEVEIEKFNAFFVEKEEEYVITWKELQDKVAWAKNSDVELMSVGREIV 897
            +V+KEV DFL+LLEVEI+KFNAFFVE+EEEY+I WKELQD+VA AK+S++ELMSVGREIV
Sbjct: 61   DVSKEVKDFLRLLEVEIDKFNAFFVEQEEEYIIKWKELQDRVAVAKDSNMELMSVGREIV 120

Query: 896  DFHGEMVLLENYSALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLNKLVKE 717
            DFHGEMVLLENYSALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLNKLVKE
Sbjct: 121  DFHGEMVLLENYSALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLNKLVKE 180

Query: 716  CEVMLSILFPKSGPLGPSLSTSEVFEEDACGSVTANENKEALEQVPRDFAEIQNMENVFI 537
            CEVMLSILFPK+  L PSLS SE FEED C + T NENKE L  VP++ +EI+NMEN+F+
Sbjct: 181  CEVMLSILFPKNRSLVPSLSISEDFEEDGCTTTTVNENKETLVHVPKELSEIENMENMFM 240

Query: 536  KLTTSALDTLKEIRGGSST 480
            KLT SAL TL++IRGGSST
Sbjct: 241  KLTQSALQTLEQIRGGSST 259


>KYP43409.1 Xenotropic and polytropic retrovirus receptor 1 isogeny [Cajanus
            cajan]
          Length = 253

 Score =  357 bits (916), Expect = e-119
 Identities = 191/265 (72%), Positives = 216/265 (81%), Gaps = 3/265 (1%)
 Frame = -2

Query: 1235 MKFWKILSNQIEQTLPDWRDKFLSYKDLKKQLKLIAPNDSSSNKRRRFDDEGGEVTKEVN 1056
            MKFWKIL +QIEQTLP+WRD+FLSYKDLKK LK++ P D+ +  R   D E      E+N
Sbjct: 1    MKFWKILKSQIEQTLPEWRDQFLSYKDLKKLLKVMCPKDALTPPR--LDAE------ELN 52

Query: 1055 DFLKLLEVEIEKFNAFFVEKEEEYVITWKELQDKVAWAKNSDVELMSVGREIVDFHGEMV 876
             FL LLE+EI+KFNAFFV+KEEEY+I WKELQD+VA A +S+VELMS+GREIVDFHGEMV
Sbjct: 53   HFLHLLELEIDKFNAFFVDKEEEYIIKWKELQDRVARAVDSNVELMSLGREIVDFHGEMV 112

Query: 875  LLENYSALNY---TGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLNKLVKECEVM 705
            LLENY+ALNY   TGLVKIIKKYDKRTGAL RLPFIQDVLNQPFFKIDVLNKLVKECEV+
Sbjct: 113  LLENYTALNYTGITGLVKIIKKYDKRTGALHRLPFIQDVLNQPFFKIDVLNKLVKECEVI 172

Query: 704  LSILFPKSGPLGPSLSTSEVFEEDACGSVTANENKEALEQVPRDFAEIQNMENVFIKLTT 525
            LSILF         LS  E +EED CGSVT NENKE L  VPR+ AEI+NME+ F KLT 
Sbjct: 173  LSILFTNDW-----LSIGEDYEEDECGSVTENENKETLMHVPRELAEIENMESTFTKLTL 227

Query: 524  SALDTLKEIRGGSSTVSIYSLPPLH 450
            SAL +L+EIRG SSTVSI+SLPPLH
Sbjct: 228  SALRSLEEIRGRSSTVSIFSLPPLH 252


>XP_014629815.1 PREDICTED: uncharacterized protein LOC100499977 isoform X1 [Glycine
            max] KRH61780.1 hypothetical protein GLYMA_04G067400
            [Glycine max] KRH61781.1 hypothetical protein
            GLYMA_04G067400 [Glycine max]
          Length = 250

 Score =  357 bits (915), Expect = e-119
 Identities = 185/263 (70%), Positives = 214/263 (81%)
 Frame = -2

Query: 1235 MKFWKILSNQIEQTLPDWRDKFLSYKDLKKQLKLIAPNDSSSNKRRRFDDEGGEVTKEVN 1056
            MKFWKIL +QIEQTLP+WRD+FLSYKDLKKQLK++ P D+ +      D        E+N
Sbjct: 1    MKFWKILKSQIEQTLPEWRDQFLSYKDLKKQLKVMCPKDALTPPCLDAD--------ELN 52

Query: 1055 DFLKLLEVEIEKFNAFFVEKEEEYVITWKELQDKVAWAKNSDVELMSVGREIVDFHGEMV 876
             FL LLE+EI+KFN FFV+KEEEY+I WKELQD+VA A +S+ ELMS+GREIVDFHGEMV
Sbjct: 53   HFLGLLELEIDKFNGFFVDKEEEYIIKWKELQDRVARAIDSNAELMSLGREIVDFHGEMV 112

Query: 875  LLENYSALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLNKLVKECEVMLSI 696
            LLENY+ALNYTGLVKIIKKYDKRTGAL+RLPFIQ+VLNQPFFKIDVLNKLVKECEV+LSI
Sbjct: 113  LLENYTALNYTGLVKIIKKYDKRTGALLRLPFIQEVLNQPFFKIDVLNKLVKECEVILSI 172

Query: 695  LFPKSGPLGPSLSTSEVFEEDACGSVTANENKEALEQVPRDFAEIQNMENVFIKLTTSAL 516
            LF          S SE FEED CGS++ NENKE L  VP++  EI+NMEN F KLT SAL
Sbjct: 173  LFTNDWS-----SISEDFEEDECGSMSGNENKETLMHVPKELDEIENMENTFTKLTLSAL 227

Query: 515  DTLKEIRGGSSTVSIYSLPPLHS 447
             +L+EIRG SSTVSI+SLPPLH+
Sbjct: 228  RSLEEIRGRSSTVSIFSLPPLHN 250


>NP_001235083.1 uncharacterized protein LOC100499977 [Glycine max] ACU14463.1 unknown
            [Glycine max]
          Length = 250

 Score =  356 bits (913), Expect = e-118
 Identities = 184/263 (69%), Positives = 214/263 (81%)
 Frame = -2

Query: 1235 MKFWKILSNQIEQTLPDWRDKFLSYKDLKKQLKLIAPNDSSSNKRRRFDDEGGEVTKEVN 1056
            MKFWKIL +QIEQTLP+WRD+FLSYKDLKKQLK++ P D+ +      D        E+N
Sbjct: 1    MKFWKILKSQIEQTLPEWRDQFLSYKDLKKQLKVMCPKDALTPPCLDAD--------ELN 52

Query: 1055 DFLKLLEVEIEKFNAFFVEKEEEYVITWKELQDKVAWAKNSDVELMSVGREIVDFHGEMV 876
             FL LLE+EI+KFN FFV+KEEEY+I WKELQD+VA A +S+ ELMS+GREIVDFHGEMV
Sbjct: 53   HFLGLLELEIDKFNGFFVDKEEEYIIKWKELQDRVARAIDSNAELMSLGREIVDFHGEMV 112

Query: 875  LLENYSALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLNKLVKECEVMLSI 696
            LLENY+ALNYTGLVKIIKKYDKRTGAL+RLPF+Q+VLNQPFFKIDVLNKLVKECEV+LSI
Sbjct: 113  LLENYTALNYTGLVKIIKKYDKRTGALLRLPFLQEVLNQPFFKIDVLNKLVKECEVILSI 172

Query: 695  LFPKSGPLGPSLSTSEVFEEDACGSVTANENKEALEQVPRDFAEIQNMENVFIKLTTSAL 516
            LF          S SE FEED CGS++ NENKE L  VP++  EI+NMEN F KLT SAL
Sbjct: 173  LFTNDWS-----SISEDFEEDECGSMSGNENKETLMHVPKELDEIENMENTFTKLTLSAL 227

Query: 515  DTLKEIRGGSSTVSIYSLPPLHS 447
             +L+EIRG SSTVSI+SLPPLH+
Sbjct: 228  RSLEEIRGRSSTVSIFSLPPLHN 250


>KRH52450.1 hypothetical protein GLYMA_06G069000 [Glycine max]
          Length = 250

 Score =  355 bits (911), Expect = e-118
 Identities = 185/263 (70%), Positives = 214/263 (81%)
 Frame = -2

Query: 1235 MKFWKILSNQIEQTLPDWRDKFLSYKDLKKQLKLIAPNDSSSNKRRRFDDEGGEVTKEVN 1056
            MKFWKIL +QIEQTLP+WRD+FLSYKDLKKQLK++ P D+ +  R   D        E+N
Sbjct: 1    MKFWKILKSQIEQTLPEWRDQFLSYKDLKKQLKVMCPKDALTPPRLDAD--------ELN 52

Query: 1055 DFLKLLEVEIEKFNAFFVEKEEEYVITWKELQDKVAWAKNSDVELMSVGREIVDFHGEMV 876
             FL+LLE+EI+KFNAFFV+KEEEY+I WKELQD+VA A  S++ELMS+GR IVDFHGEMV
Sbjct: 53   HFLRLLELEIDKFNAFFVDKEEEYIIKWKELQDRVARAIGSNLELMSLGRGIVDFHGEMV 112

Query: 875  LLENYSALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLNKLVKECEVMLSI 696
            LLENY+ALNYTGLVKIIKKYDKRTGAL+RLPFIQ+VLNQPFFKIDVLNKLVKECEV+LSI
Sbjct: 113  LLENYTALNYTGLVKIIKKYDKRTGALLRLPFIQEVLNQPFFKIDVLNKLVKECEVILSI 172

Query: 695  LFPKSGPLGPSLSTSEVFEEDACGSVTANENKEALEQVPRDFAEIQNMENVFIKLTTSAL 516
            LF    P     S S  FEED  GS T NE+K  L  VP++ AEI+NMEN F KLT SAL
Sbjct: 173  LFNNDWP-----SISGDFEEDEYGSTTGNESKATLMHVPKELAEIENMENTFTKLTLSAL 227

Query: 515  DTLKEIRGGSSTVSIYSLPPLHS 447
             +L+EIRG SSTVSI+SLPPLH+
Sbjct: 228  RSLEEIRGRSSTVSIFSLPPLHN 250


>KHN07083.1 SPX domain-containing protein 1 [Glycine soja]
          Length = 251

 Score =  350 bits (899), Expect = e-116
 Identities = 185/264 (70%), Positives = 214/264 (81%), Gaps = 1/264 (0%)
 Frame = -2

Query: 1235 MKFWKILSNQIEQTLPDWRDKFLSYKDLKKQLKLIAPNDSSSNKRRRFDDEGGEVTKEVN 1056
            MKFWKIL +QIEQTLP+WRD+FLSYKDLKKQLK++ P D+ +  R   D        E+N
Sbjct: 1    MKFWKILKSQIEQTLPEWRDQFLSYKDLKKQLKVMCPKDALTPPRLDAD--------ELN 52

Query: 1055 DFLKLLEVEIEKFNAFFVEKEEEYVITWKELQDKVAWAKNSDVELMSVGREIVDFHGEMV 876
             FL+LLE+EI+KFNAFFV+KEEEY+I WKELQD+VA A  S++ELMS+GR IVDFHGEMV
Sbjct: 53   HFLRLLELEIDKFNAFFVDKEEEYIIKWKELQDRVARAIGSNLELMSLGRGIVDFHGEMV 112

Query: 875  LLENYSALNYTG-LVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLNKLVKECEVMLS 699
            LLENY+ALNYTG LVKIIKKYDKRTGAL+RLPFIQ+VLNQPFFKIDVLNKLVKECEV+LS
Sbjct: 113  LLENYTALNYTGSLVKIIKKYDKRTGALLRLPFIQEVLNQPFFKIDVLNKLVKECEVILS 172

Query: 698  ILFPKSGPLGPSLSTSEVFEEDACGSVTANENKEALEQVPRDFAEIQNMENVFIKLTTSA 519
            ILF    P     S S  FEED  GS T NE+K  L  VP++ AEI+NMEN F KLT SA
Sbjct: 173  ILFNNDWP-----SISGDFEEDEYGSTTGNESKATLMHVPKELAEIENMENTFTKLTLSA 227

Query: 518  LDTLKEIRGGSSTVSIYSLPPLHS 447
            L +L+EIRG SSTVSI+SLPPLH+
Sbjct: 228  LRSLEEIRGRSSTVSIFSLPPLHN 251


>XP_009355732.1 PREDICTED: SPX domain-containing protein 1-like [Pyrus x
            bretschneideri]
          Length = 288

 Score =  339 bits (870), Expect = e-111
 Identities = 179/279 (64%), Positives = 211/279 (75%), Gaps = 10/279 (3%)
 Frame = -2

Query: 1235 MKFWKILSNQIEQTLPDWRDKFLSYKDLKKQLKLIAPNDSSS--NKRRRFDD-------- 1086
            MKF K L   IE+ LP+WRDKFLSYKDLKKQLKLI P D     NKR R  D        
Sbjct: 1    MKFSKSLGILIEEALPEWRDKFLSYKDLKKQLKLIYPKDGDKPPNKRPRLSDDDDDRADG 60

Query: 1085 EGGEVTKEVNDFLKLLEVEIEKFNAFFVEKEEEYVITWKELQDKVAWAKNSDVELMSVGR 906
            EGGEV+KEV DF++LLE E+EKFNAFF EKEE+YVI WKELQD+VA AK+S+ ELM+VGR
Sbjct: 61   EGGEVSKEVTDFVRLLENELEKFNAFFEEKEEDYVIRWKELQDRVADAKDSNEELMNVGR 120

Query: 905  EIVDFHGEMVLLENYSALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLNKL 726
            EIVDFHGEMVLLENYSALNYTGL+KI+KK+DKRTGALIRLPF+Q V+ QPFF  DVLNKL
Sbjct: 121  EIVDFHGEMVLLENYSALNYTGLLKILKKHDKRTGALIRLPFVQRVMQQPFFTTDVLNKL 180

Query: 725  VKECEVMLSILFPKSGPLGPSLSTSEVFEEDACGSVTANENKEALEQVPRDFAEIQNMEN 546
            VKECEVML  +F K+ P  PS +T     E+ C S T  EN++ + + P + AEI+ ME+
Sbjct: 181  VKECEVMLDHVFSKNDPSAPSEATE---VEERCESTTVTENRDRIFKAPTELAEIKQMES 237

Query: 545  VFIKLTTSALDTLKEIRGGSSTVSIYSLPPLHSETLVED 429
            V++K T SAL  LKE+R GSSTVS +SLPPL    + ED
Sbjct: 238  VYVKQTVSALRVLKEVRSGSSTVSAFSLPPLQINVMEED 276


>XP_008394070.1 PREDICTED: SPX domain-containing protein 1-like [Malus domestica]
          Length = 288

 Score =  335 bits (859), Expect = e-110
 Identities = 177/279 (63%), Positives = 209/279 (74%), Gaps = 10/279 (3%)
 Frame = -2

Query: 1235 MKFWKILSNQIEQTLPDWRDKFLSYKDLKKQLKLIAPNDSSS--NKRRRFDD-------- 1086
            MKF K L   IE+ LP+WRDKFLSYKDLKKQLKLI P D     NKR R  D        
Sbjct: 1    MKFSKSLGILIEEALPEWRDKFLSYKDLKKQLKLIYPKDGDKPPNKRPRXSDDDDDRADG 60

Query: 1085 EGGEVTKEVNDFLKLLEVEIEKFNAFFVEKEEEYVITWKELQDKVAWAKNSDVELMSVGR 906
            EGGEV+KEV DF++LLE E+EKFNAFF EKEE+YVI WKELQD+VA AK+S+ ELM+VGR
Sbjct: 61   EGGEVSKEVTDFVRLLENELEKFNAFFEEKEEDYVIRWKELQDRVADAKDSNEELMNVGR 120

Query: 905  EIVDFHGEMVLLENYSALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLNKL 726
            EIVDFHGEMVLLENYSALNYTGL+KI+KK+DKRTGALIRLPF+Q V+ QPFF  DVLNKL
Sbjct: 121  EIVDFHGEMVLLENYSALNYTGLLKILKKHDKRTGALIRLPFVQRVMQQPFFTTDVLNKL 180

Query: 725  VKECEVMLSILFPKSGPLGPSLSTSEVFEEDACGSVTANENKEALEQVPRDFAEIQNMEN 546
            VKECEVML  +F K+ P  PS +T     E+ C S     N++ + + P + AEI+ ME+
Sbjct: 181  VKECEVMLDHVFSKNDPSAPSEATK---VEERCESTAVTXNRDRIFKAPTELAEIKQMES 237

Query: 545  VFIKLTTSALDTLKEIRGGSSTVSIYSLPPLHSETLVED 429
            V++K T SAL  LKE+R GSSTVS +SLPPL    + ED
Sbjct: 238  VYVKQTVSALRVLKEVRSGSSTVSAFSLPPLQMNVMEED 276


>XP_007011873.2 PREDICTED: SPX domain-containing protein 2 [Theobroma cacao]
          Length = 293

 Score =  333 bits (853), Expect = e-109
 Identities = 180/281 (64%), Positives = 212/281 (75%), Gaps = 13/281 (4%)
 Frame = -2

Query: 1235 MKFWKILSNQIEQTLPDWRDKFLSYKDLKKQLKLIAPNDSSS---NKRRRFDD-EGG--- 1077
            MKFWK LS  IE+ LPDWRDKFLSYKDLKKQLKLI P D S    +KR R D  EGG   
Sbjct: 1    MKFWKSLSILIEEALPDWRDKFLSYKDLKKQLKLIYPKDGSEKQVSKRPRLDSAEGGDGG 60

Query: 1076 -----EVTKEVNDFLKLLEVEIEKFNAFFVEKEEEYVITWKELQDKVAWAKNSDVELMSV 912
                 EV KEV DF+ LLE EIEKFNAFF+EKEE+YVI W+ELQD+ A AK S  ELM V
Sbjct: 61   SKEESEVPKEVMDFVLLLEDEIEKFNAFFIEKEEDYVIKWRELQDRAAKAKVSKGELMKV 120

Query: 911  GREIVDFHGEMVLLENYSALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLN 732
            GREIVDFHGEMVLLENYSALNYTGLVKIIKKYDKR+GAL+RLPFI+ VL QPF++ DVLN
Sbjct: 121  GREIVDFHGEMVLLENYSALNYTGLVKIIKKYDKRSGALVRLPFIRKVLQQPFYRTDVLN 180

Query: 731  KLVKECEVMLSILFPKSGP-LGPSLSTSEVFEEDACGSVTANENKEALEQVPRDFAEIQN 555
            +LVKECE++L  LF  + P   P  +  +V  E  C S T+  + E L +VP++ AEI+N
Sbjct: 181  QLVKECEMVLDHLFSMNEPSASPEATDEKVTSEKECESKTSTRSTERLLKVPKELAEIKN 240

Query: 554  MENVFIKLTTSALDTLKEIRGGSSTVSIYSLPPLHSETLVE 432
            M+++++KLT SAL  LKEIR GSSTVS++SLPPL +  L E
Sbjct: 241  MKSMYMKLTLSALHVLKEIRSGSSTVSMFSLPPLQNHALEE 281


>XP_018822225.1 PREDICTED: SPX domain-containing protein 1-like [Juglans regia]
          Length = 288

 Score =  332 bits (852), Expect = e-109
 Identities = 171/277 (61%), Positives = 214/277 (77%), Gaps = 12/277 (4%)
 Frame = -2

Query: 1235 MKFWKILSNQIEQTLPDWRDKFLSYKDLKKQLKLIAPNDSSSNK----RRRFDDEGG--- 1077
            MKFWKIL++ IE+TLP+WRDKFLSYKDLKKQLKLI P D  ++K    R R D +     
Sbjct: 1    MKFWKILNSLIEETLPEWRDKFLSYKDLKKQLKLIYPKDDDTHKMPDKRPRLDSDPNVDA 60

Query: 1076 -----EVTKEVNDFLKLLEVEIEKFNAFFVEKEEEYVITWKELQDKVAWAKNSDVELMSV 912
                 E++KEV+DF++LLE EI+KFN FFV+KEEEYVI WKELQD++A AK+S+ ELM V
Sbjct: 61   SYCPPELSKEVDDFVRLLEDEIDKFNGFFVDKEEEYVIRWKELQDRIAKAKDSNEELMQV 120

Query: 911  GREIVDFHGEMVLLENYSALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLN 732
            GRE+VD HGEMVLLENYSALNYTGLVKI+KKY+KR+GALIRLPFIQ V+ QPFF  DVLN
Sbjct: 121  GREVVDLHGEMVLLENYSALNYTGLVKILKKYEKRSGALIRLPFIQRVMQQPFFTTDVLN 180

Query: 731  KLVKECEVMLSILFPKSGPLGPSLSTSEVFEEDACGSVTANENKEALEQVPRDFAEIQNM 552
            KLVKECE ML  +F ++ P GP   T+    E+   S    E+K+   +VP++  EI++M
Sbjct: 181  KLVKECESMLDQIFSRNDPSGPLEGTN---NEEGSDSKAVTESKKTQLKVPKELEEIKHM 237

Query: 551  ENVFIKLTTSALDTLKEIRGGSSTVSIYSLPPLHSET 441
            E++++KLT SAL  LKE+RGGSSTVS++SLPPL + T
Sbjct: 238  ESMYMKLTISALRVLKEVRGGSSTVSVFSLPPLQNNT 274


>EOY29492.1 SPX domain-containing protein 2 [Theobroma cacao]
          Length = 293

 Score =  332 bits (851), Expect = e-108
 Identities = 178/281 (63%), Positives = 210/281 (74%), Gaps = 13/281 (4%)
 Frame = -2

Query: 1235 MKFWKILSNQIEQTLPDWRDKFLSYKDLKKQLKLIAPNDSSS---NKRRRFDDEGG---- 1077
            MKFWK LS  IE+ LPDWRDKFLSYKDLKKQLKLI P D S    +KR R D   G    
Sbjct: 1    MKFWKSLSILIEEALPDWRDKFLSYKDLKKQLKLIYPKDGSEKQVSKRPRLDSADGGDGG 60

Query: 1076 -----EVTKEVNDFLKLLEVEIEKFNAFFVEKEEEYVITWKELQDKVAWAKNSDVELMSV 912
                 EV KEV DF+ LLE EIEKFNAFF+EKEE+YVI W+ELQD+ A AK S  ELM V
Sbjct: 61   SKEESEVPKEVMDFVLLLEDEIEKFNAFFIEKEEDYVIKWRELQDRAAKAKVSKGELMKV 120

Query: 911  GREIVDFHGEMVLLENYSALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPFFKIDVLN 732
            GREIVDFHGEMVLLENYSALNYTGLVKIIKKYDKR+GAL+RLPFI+ VL QPF++ DVLN
Sbjct: 121  GREIVDFHGEMVLLENYSALNYTGLVKIIKKYDKRSGALVRLPFIRKVLQQPFYRTDVLN 180

Query: 731  KLVKECEVMLSILFPKSGP-LGPSLSTSEVFEEDACGSVTANENKEALEQVPRDFAEIQN 555
            +LVKECE++L  LF  + P   P  +  +V  E  C S T+  + E L +VP++ AEI+N
Sbjct: 181  QLVKECEMVLDHLFSMNEPSASPEATDEKVTSEKECESKTSTRSTERLLKVPKELAEIKN 240

Query: 554  MENVFIKLTTSALDTLKEIRGGSSTVSIYSLPPLHSETLVE 432
            M+++++KLT SAL  LKEIR GSSTVS++SLPPL +  L E
Sbjct: 241  MKSMYMKLTLSALHVLKEIRSGSSTVSMFSLPPLQNHALEE 281


>OAY27376.1 hypothetical protein MANES_16G121300 [Manihot esculenta]
          Length = 297

 Score =  332 bits (851), Expect = e-108
 Identities = 177/294 (60%), Positives = 216/294 (73%), Gaps = 19/294 (6%)
 Frame = -2

Query: 1235 MKFWKILSNQIEQTLPDWRDKFLSYKDLKKQLKLIAPNDSSS--NKRRRFD--------- 1089
            MKFWK LS  IE TLP+WRDKFLSYKDLKKQLKLI P D     NKR R +         
Sbjct: 1    MKFWKSLSILIEDTLPEWRDKFLSYKDLKKQLKLIYPKDGDKPPNKRPRLEAVMVASTDR 60

Query: 1088 --------DEGGEVTKEVNDFLKLLEVEIEKFNAFFVEKEEEYVITWKELQDKVAWAKNS 933
                    + GGEV KEV DF++LLE E+EKFN+F VEKEE++VI WKELQD+V  AK+S
Sbjct: 61   KDGEYRREESGGEVMKEVIDFVRLLEDEMEKFNSFIVEKEEDFVIQWKELQDRVKKAKDS 120

Query: 932  DVELMSVGREIVDFHGEMVLLENYSALNYTGLVKIIKKYDKRTGALIRLPFIQDVLNQPF 753
            + +LM VGREIV+FHGEMVLLENYSALNYTGLVKI+KKYDKR+GAL+RLPFIQ V+ QPF
Sbjct: 121  NEKLMEVGREIVNFHGEMVLLENYSALNYTGLVKILKKYDKRSGALVRLPFIQKVMQQPF 180

Query: 752  FKIDVLNKLVKECEVMLSILFPKSGPLGPSLSTSEVFEEDACGSVTANENKEALEQVPRD 573
            F   VLNKLVKECE ML  LF ++     S+S  E  E + C S T+ E++    +VP++
Sbjct: 181  FTTHVLNKLVKECETMLDRLFSRN---ELSISPDETEEVEGCDSKTSAESRGQSIEVPKE 237

Query: 572  FAEIQNMENVFIKLTTSALDTLKEIRGGSSTVSIYSLPPLHSETLVED*RKITL 411
             AEI+NMEN+++KLT SAL  LKE+R GSSTV+++SLPPL S  L ED +K+ +
Sbjct: 238  LAEIENMENMYLKLTLSALRVLKEVRSGSSTVNMFSLPPLQSRALEEDWKKVPI 291