BLASTX nr result
ID: Glycyrrhiza31_contig00004529
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Glycyrrhiza31_contig00004529
(546 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
XP_012834213.1 PREDICTED: calcium-transporting ATPase 1, endopla... 154 2e-44
EYU40083.1 hypothetical protein MIMGU_mgv1a018305mg, partial [Er... 154 3e-44
KHN09451.1 Calcium-transporting ATPase 4, endoplasmic reticulum-... 158 1e-41
XP_003554341.1 PREDICTED: calcium-transporting ATPase 4, endopla... 158 1e-41
XP_003521357.1 PREDICTED: calcium-transporting ATPase 1, endopla... 158 1e-41
XP_019444380.1 PREDICTED: calcium-transporting ATPase 1, endopla... 154 3e-40
XP_004493912.1 PREDICTED: calcium-transporting ATPase 4, endopla... 154 3e-40
XP_019456196.1 PREDICTED: calcium-transporting ATPase 1, endopla... 154 3e-40
KDO42782.1 hypothetical protein CISIN_1g010234mg [Citrus sinensis] 151 4e-40
XP_014495779.1 PREDICTED: calcium-transporting ATPase 4, endopla... 154 5e-40
XP_003625525.2 endoplasmic reticulum [ER]-type calcium ATPase, p... 153 1e-39
DAA34949.1 TPA_exp: type IIA calcium ATPase [Medicago truncatula] 153 1e-39
XP_007162693.1 hypothetical protein PHAVU_001G172300g [Phaseolus... 152 1e-39
KYP70818.1 Calcium-transporting ATPase 4, endoplasmic reticulum-... 152 2e-39
AAM91535.1 endoplasmic reticulum-type calcium-transporting ATPas... 146 3e-39
BAG88475.1 unnamed protein product [Oryza sativa Japonica Group] 143 4e-39
XP_017418505.1 PREDICTED: calcium-transporting ATPase 4, endopla... 151 5e-39
XP_006480711.1 PREDICTED: calcium-transporting ATPase 4, endopla... 151 5e-39
XP_006428976.1 hypothetical protein CICLE_v10010954mg [Citrus cl... 151 5e-39
BAF16406.1 Os05g0120700, partial [Oryza sativa Japonica Group] B... 143 6e-39
>XP_012834213.1 PREDICTED: calcium-transporting ATPase 1, endoplasmic
reticulum-type-like, partial [Erythranthe guttata]
Length = 168
Score = 154 bits (388), Expect = 2e-44
Identities = 78/100 (78%), Positives = 82/100 (82%), Gaps = 2/100 (2%)
Frame = -3
Query: 544 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWX 365
MFNSLNALSEDGSLLTMPPWVNPWLLLAMS+SFGLHFLILYVPFLAQ+FGIVPLS NEW
Sbjct: 69 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSISFGLHFLILYVPFLAQIFGIVPLSLNEWL 128
Query: 364 XXXXXXXXXXXIDEVLKFVGRCTS--RYQASSRRSKQKSE 251
IDEVLKFVGRCTS RY +S R SKQK+E
Sbjct: 129 LVLAVALPVIVIDEVLKFVGRCTSGIRYSSSKRYSKQKAE 168
>EYU40083.1 hypothetical protein MIMGU_mgv1a018305mg, partial [Erythranthe
guttata]
Length = 189
Score = 154 bits (388), Expect = 3e-44
Identities = 78/100 (78%), Positives = 82/100 (82%), Gaps = 2/100 (2%)
Frame = -3
Query: 544 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWX 365
MFNSLNALSEDGSLLTMPPWVNPWLLLAMS+SFGLHFLILYVPFLAQ+FGIVPLS NEW
Sbjct: 90 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSISFGLHFLILYVPFLAQIFGIVPLSLNEWL 149
Query: 364 XXXXXXXXXXXIDEVLKFVGRCTS--RYQASSRRSKQKSE 251
IDEVLKFVGRCTS RY +S R SKQK+E
Sbjct: 150 LVLAVALPVIVIDEVLKFVGRCTSGIRYSSSKRYSKQKAE 189
>KHN09451.1 Calcium-transporting ATPase 4, endoplasmic reticulum-type [Glycine
soja]
Length = 1060
Score = 158 bits (400), Expect = 1e-41
Identities = 79/98 (80%), Positives = 83/98 (84%)
Frame = -3
Query: 544 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWX 365
MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEW
Sbjct: 963 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWL 1022
Query: 364 XXXXXXXXXXXIDEVLKFVGRCTSRYQASSRRSKQKSE 251
IDE+LKFVGRCTS +S+R+SKQKSE
Sbjct: 1023 LVLVVALPVILIDEILKFVGRCTSSRASSARKSKQKSE 1060
>XP_003554341.1 PREDICTED: calcium-transporting ATPase 4, endoplasmic
reticulum-type-like [Glycine max] KHN02394.1
Calcium-transporting ATPase 4, endoplasmic reticulum-type
[Glycine soja] KRG95874.1 hypothetical protein
GLYMA_19G175900 [Glycine max]
Length = 1060
Score = 158 bits (400), Expect = 1e-41
Identities = 79/98 (80%), Positives = 83/98 (84%)
Frame = -3
Query: 544 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWX 365
MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEW
Sbjct: 963 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWL 1022
Query: 364 XXXXXXXXXXXIDEVLKFVGRCTSRYQASSRRSKQKSE 251
IDE+LKFVGRCTS +S+R+SKQKSE
Sbjct: 1023 LVLVVALPVILIDEILKFVGRCTSSRASSARKSKQKSE 1060
>XP_003521357.1 PREDICTED: calcium-transporting ATPase 1, endoplasmic
reticulum-type-like [Glycine max] KRH67591.1 hypothetical
protein GLYMA_03G175200 [Glycine max]
Length = 1060
Score = 158 bits (400), Expect = 1e-41
Identities = 79/98 (80%), Positives = 83/98 (84%)
Frame = -3
Query: 544 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWX 365
MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEW
Sbjct: 963 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWL 1022
Query: 364 XXXXXXXXXXXIDEVLKFVGRCTSRYQASSRRSKQKSE 251
IDE+LKFVGRCTS +S+R+SKQKSE
Sbjct: 1023 LVLVVALPVILIDEILKFVGRCTSSRASSARKSKQKSE 1060
>XP_019444380.1 PREDICTED: calcium-transporting ATPase 1, endoplasmic
reticulum-type-like [Lupinus angustifolius] OIW11219.1
hypothetical protein TanjilG_28310 [Lupinus
angustifolius]
Length = 1052
Score = 154 bits (390), Expect = 3e-40
Identities = 80/100 (80%), Positives = 83/100 (83%), Gaps = 2/100 (2%)
Frame = -3
Query: 544 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWX 365
MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEW
Sbjct: 953 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWL 1012
Query: 364 XXXXXXXXXXXIDEVLKFVGRCTSR--YQASSRRSKQKSE 251
IDE+LKFVGRCTS QA++ RSKQKSE
Sbjct: 1013 LVLAVALPVILIDEILKFVGRCTSTSGSQAAAARSKQKSE 1052
>XP_004493912.1 PREDICTED: calcium-transporting ATPase 4, endoplasmic
reticulum-type-like [Cicer arietinum]
Length = 1058
Score = 154 bits (390), Expect = 3e-40
Identities = 79/98 (80%), Positives = 82/98 (83%)
Frame = -3
Query: 544 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWX 365
MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHF+ILYVPFLAQVFGIVPLSFNEW
Sbjct: 964 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFIILYVPFLAQVFGIVPLSFNEWL 1023
Query: 364 XXXXXXXXXXXIDEVLKFVGRCTSRYQASSRRSKQKSE 251
IDE+LKFVGRCTS S+RRSKQKSE
Sbjct: 1024 LVLAVALPVILIDEILKFVGRCTS---GSARRSKQKSE 1058
>XP_019456196.1 PREDICTED: calcium-transporting ATPase 1, endoplasmic
reticulum-type-like [Lupinus angustifolius]
XP_019456197.1 PREDICTED: calcium-transporting ATPase 1,
endoplasmic reticulum-type-like [Lupinus angustifolius]
OIW05095.1 hypothetical protein TanjilG_06231 [Lupinus
angustifolius]
Length = 1062
Score = 154 bits (390), Expect = 3e-40
Identities = 80/100 (80%), Positives = 83/100 (83%), Gaps = 2/100 (2%)
Frame = -3
Query: 544 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWX 365
MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEW
Sbjct: 963 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWL 1022
Query: 364 XXXXXXXXXXXIDEVLKFVGRCTSR--YQASSRRSKQKSE 251
IDE+LKFVGRCTS QA++ RSKQKSE
Sbjct: 1023 LVLAVALPVILIDEILKFVGRCTSTSGSQAAAARSKQKSE 1062
>KDO42782.1 hypothetical protein CISIN_1g010234mg [Citrus sinensis]
Length = 514
Score = 151 bits (381), Expect = 4e-40
Identities = 78/99 (78%), Positives = 81/99 (81%), Gaps = 1/99 (1%)
Frame = -3
Query: 544 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWX 365
MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQ+FGIVPLSFNEW
Sbjct: 416 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQIFGIVPLSFNEWL 475
Query: 364 XXXXXXXXXXXIDEVLKFVGRCTSRYQASSRR-SKQKSE 251
IDEVLKFVGRCT+ Q S R+ SK KSE
Sbjct: 476 LVLAIAFPVVLIDEVLKFVGRCTNGSQTSRRKSSKPKSE 514
>XP_014495779.1 PREDICTED: calcium-transporting ATPase 4, endoplasmic
reticulum-type-like [Vigna radiata var. radiata]
Length = 1061
Score = 154 bits (388), Expect = 5e-40
Identities = 79/99 (79%), Positives = 83/99 (83%), Gaps = 1/99 (1%)
Frame = -3
Query: 544 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWX 365
MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLS NEW
Sbjct: 963 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSLNEWL 1022
Query: 364 XXXXXXXXXXXIDEVLKFVGRCTS-RYQASSRRSKQKSE 251
IDE+LKFVGRCTS R ++SR+SKQKSE
Sbjct: 1023 LVLAVALPVILIDEILKFVGRCTSGRRPSTSRKSKQKSE 1061
>XP_003625525.2 endoplasmic reticulum [ER]-type calcium ATPase, putative [Medicago
truncatula] AES81743.2 endoplasmic reticulum [ER]-type
calcium ATPase, putative [Medicago truncatula]
Length = 1072
Score = 153 bits (386), Expect = 1e-39
Identities = 79/98 (80%), Positives = 82/98 (83%)
Frame = -3
Query: 544 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWX 365
MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLA+VFGIVPLSFNEW
Sbjct: 978 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAKVFGIVPLSFNEWL 1037
Query: 364 XXXXXXXXXXXIDEVLKFVGRCTSRYQASSRRSKQKSE 251
IDEVLKFVGRCTS S+RRSKQKS+
Sbjct: 1038 LVLAVALPVILIDEVLKFVGRCTS---GSARRSKQKSD 1072
>DAA34949.1 TPA_exp: type IIA calcium ATPase [Medicago truncatula]
Length = 1081
Score = 153 bits (386), Expect = 1e-39
Identities = 79/98 (80%), Positives = 82/98 (83%)
Frame = -3
Query: 544 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWX 365
MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLA+VFGIVPLSFNEW
Sbjct: 987 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAKVFGIVPLSFNEWL 1046
Query: 364 XXXXXXXXXXXIDEVLKFVGRCTSRYQASSRRSKQKSE 251
IDEVLKFVGRCTS S+RRSKQKS+
Sbjct: 1047 LVLAVALPVILIDEVLKFVGRCTS---GSARRSKQKSD 1081
>XP_007162693.1 hypothetical protein PHAVU_001G172300g [Phaseolus vulgaris]
ESW34687.1 hypothetical protein PHAVU_001G172300g
[Phaseolus vulgaris]
Length = 1059
Score = 152 bits (385), Expect = 1e-39
Identities = 76/98 (77%), Positives = 82/98 (83%)
Frame = -3
Query: 544 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWX 365
MFNSLNALSEDGSLL+MPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLS NEW
Sbjct: 962 MFNSLNALSEDGSLLSMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSLNEWL 1021
Query: 364 XXXXXXXXXXXIDEVLKFVGRCTSRYQASSRRSKQKSE 251
IDE+LKFVGRCTS + ++R+SKQKSE
Sbjct: 1022 LVLAVALPVILIDEILKFVGRCTSGPRPAARKSKQKSE 1059
>KYP70818.1 Calcium-transporting ATPase 4, endoplasmic reticulum-type [Cajanus
cajan]
Length = 1056
Score = 152 bits (384), Expect = 2e-39
Identities = 78/98 (79%), Positives = 82/98 (83%)
Frame = -3
Query: 544 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWX 365
MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEW
Sbjct: 961 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWL 1020
Query: 364 XXXXXXXXXXXIDEVLKFVGRCTSRYQASSRRSKQKSE 251
IDE+LKFVGRCTS + +RRSKQK+E
Sbjct: 1021 LVLVVALPVILIDEILKFVGRCTS--GSRTRRSKQKTE 1056
>AAM91535.1 endoplasmic reticulum-type calcium-transporting ATPase 4
[Arabidopsis thaliana]
Length = 375
Score = 146 bits (368), Expect = 3e-39
Identities = 75/100 (75%), Positives = 80/100 (80%), Gaps = 2/100 (2%)
Frame = -3
Query: 544 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWX 365
MFNSLNALSEDGSL+TMPPWVNPWLLLAM+VSFGLHF+ILYVPFLAQVFGIVPLS NEW
Sbjct: 276 MFNSLNALSEDGSLVTMPPWVNPWLLLAMAVSFGLHFVILYVPFLAQVFGIVPLSLNEWL 335
Query: 364 XXXXXXXXXXXIDEVLKFVGRCTSRYQASSR--RSKQKSE 251
IDEVLKFVGRCTS Y+ S R +KQK E
Sbjct: 336 LVLAVSLPVILIDEVLKFVGRCTSGYRYSPRTPSAKQKEE 375
>BAG88475.1 unnamed protein product [Oryza sativa Japonica Group]
Length = 267
Score = 143 bits (360), Expect = 4e-39
Identities = 74/98 (75%), Positives = 74/98 (75%)
Frame = -3
Query: 544 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWX 365
MFNSLNALSED SLL MPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLS NEW
Sbjct: 170 MFNSLNALSEDTSLLRMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSLNEWL 229
Query: 364 XXXXXXXXXXXIDEVLKFVGRCTSRYQASSRRSKQKSE 251
IDEVLKFVGRCTS R KQK E
Sbjct: 230 LVLLVALPVVLIDEVLKFVGRCTSSSGPKRRTRKQKGE 267
>XP_017418505.1 PREDICTED: calcium-transporting ATPase 4, endoplasmic
reticulum-type-like [Vigna angularis] KOM38945.1
hypothetical protein LR48_Vigan03g232700 [Vigna
angularis] BAT85654.1 hypothetical protein VIGAN_04322500
[Vigna angularis var. angularis]
Length = 1059
Score = 151 bits (381), Expect = 5e-39
Identities = 78/98 (79%), Positives = 81/98 (82%)
Frame = -3
Query: 544 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWX 365
MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLS NEW
Sbjct: 963 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSLNEWL 1022
Query: 364 XXXXXXXXXXXIDEVLKFVGRCTSRYQASSRRSKQKSE 251
IDE+LKFVGRCTS + SR+SKQKSE
Sbjct: 1023 LVLAVALPVILIDEILKFVGRCTSG-RRPSRKSKQKSE 1059
>XP_006480711.1 PREDICTED: calcium-transporting ATPase 4, endoplasmic
reticulum-type-like [Citrus sinensis]
Length = 1064
Score = 151 bits (381), Expect = 5e-39
Identities = 78/99 (78%), Positives = 81/99 (81%), Gaps = 1/99 (1%)
Frame = -3
Query: 544 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWX 365
MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQ+FGIVPLSFNEW
Sbjct: 966 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQIFGIVPLSFNEWL 1025
Query: 364 XXXXXXXXXXXIDEVLKFVGRCTSRYQASSRR-SKQKSE 251
IDEVLKFVGRCT+ Q S R+ SK KSE
Sbjct: 1026 LVLAIAFPVVLIDEVLKFVGRCTNGSQTSRRKSSKPKSE 1064
>XP_006428976.1 hypothetical protein CICLE_v10010954mg [Citrus clementina] ESR42216.1
hypothetical protein CICLE_v10010954mg [Citrus
clementina]
Length = 1064
Score = 151 bits (381), Expect = 5e-39
Identities = 78/99 (78%), Positives = 81/99 (81%), Gaps = 1/99 (1%)
Frame = -3
Query: 544 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWX 365
MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQ+FGIVPLSFNEW
Sbjct: 966 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQIFGIVPLSFNEWL 1025
Query: 364 XXXXXXXXXXXIDEVLKFVGRCTSRYQASSRR-SKQKSE 251
IDEVLKFVGRCT+ Q S R+ SK KSE
Sbjct: 1026 LVLAIAFPVVLIDEVLKFVGRCTNGSQTSRRKSSKPKSE 1064
>BAF16406.1 Os05g0120700, partial [Oryza sativa Japonica Group] BAS92015.1
Os05g0120700, partial [Oryza sativa Japonica Group]
Length = 281
Score = 143 bits (360), Expect = 6e-39
Identities = 74/98 (75%), Positives = 74/98 (75%)
Frame = -3
Query: 544 MFNSLNALSEDGSLLTMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSFNEWX 365
MFNSLNALSED SLL MPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLS NEW
Sbjct: 184 MFNSLNALSEDTSLLRMPPWVNPWLLLAMSVSFGLHFLILYVPFLAQVFGIVPLSLNEWL 243
Query: 364 XXXXXXXXXXXIDEVLKFVGRCTSRYQASSRRSKQKSE 251
IDEVLKFVGRCTS R KQK E
Sbjct: 244 LVLLVALPVVLIDEVLKFVGRCTSSSGPKRRTRKQKGE 281