BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Glycyrrhiza24_contig00015102
         (2079 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003529733.1| PREDICTED: protein HOTHEAD-like [Glycine max]     944   0.0  
ref|XP_003531615.1| PREDICTED: protein HOTHEAD-like [Glycine max]     942   0.0  
ref|XP_003597604.1| Protein HOTHEAD [Medicago truncatula] gi|355...   922   0.0  
ref|XP_003546687.1| PREDICTED: protein HOTHEAD-like [Glycine max]     917   0.0  
ref|XP_003543593.1| PREDICTED: protein HOTHEAD-like [Glycine max]     915   0.0  

>ref|XP_003529733.1| PREDICTED: protein HOTHEAD-like [Glycine max]
          Length = 591

 Score =  944 bits (2441), Expect = 0.0
 Identities = 463/581 (79%), Positives = 515/581 (88%), Gaps = 1/581 (0%)
 Frame = -2

Query: 2021 MASVGAVKXXXXXXXXXXXXXL-PPCQGKQKWHEDHQYXXXXXXXXXXXXXXXXXSLNKA 1845
            MASVGAV                PP QG + WHE+ +Y                 + N A
Sbjct: 1    MASVGAVNCKFFLCLLLWLWNFLPPSQGNKHWHEN-RYPGIRNASSFSSPSISTTTGNNA 59

Query: 1844 YDYIIVGGGTAGCPLAATLSQNFSVLLLERGGVPFTNPNVTFLENFHITLADTSPTSASQ 1665
            YDYIIVGGGTAGCPLAATLSQNFSVL+LERGGVPFTNPNV+FLENFHITLAD SPTSASQ
Sbjct: 60   YDYIIVGGGTAGCPLAATLSQNFSVLVLERGGVPFTNPNVSFLENFHITLADISPTSASQ 119

Query: 1664 YFISTDGVFNARARVLGGGSSINAGFYTRASQRFVEKAGWDAKLVNESYPWVEKQIVHRP 1485
            YFISTDGV+N+RARVLGGGSSINAGFYTRA+ RF++K GWDAKLVN+SYPWVEKQIVHRP
Sbjct: 120  YFISTDGVYNSRARVLGGGSSINAGFYTRANPRFIKKVGWDAKLVNQSYPWVEKQIVHRP 179

Query: 1484 KFSAYQRAVRESLVDSGVSPFNGFTYDHIYGTKVGGTIFDRFGRRHTSAELLASGNPDKL 1305
            KFS YQRA R+SL+DSGVSPFNGFTYDH+YGTKVGGTIFDRFGRRHT+AELLASGN DKL
Sbjct: 180  KFSPYQRAFRDSLLDSGVSPFNGFTYDHLYGTKVGGTIFDRFGRRHTAAELLASGNQDKL 239

Query: 1304 TVLVHATVQKIVFDTTGKRSKAVGVIFKDENGKQHEAVLGNDIHSEVIVSSGAIGTPQLL 1125
            TVLV+ATVQKIVFDT GK+ KAVGVIF+DE+GKQHEA+L ND HSEVI+SSGAIGTPQLL
Sbjct: 240  TVLVYATVQKIVFDTRGKKPKAVGVIFQDEHGKQHEAILSNDRHSEVIMSSGAIGTPQLL 299

Query: 1124 LLSGIGPKAELKNLNISVVLDNPFVGKGMIDNPMNTIFVPSNRPVVQSLIQTVGITKLGV 945
            +LSGIGPKAEL+ LNI VVLDNPFVGKGM+DNPMNT+F+PSNRPV QSLI+TVGITK+GV
Sbjct: 300  MLSGIGPKAELQKLNIPVVLDNPFVGKGMVDNPMNTMFIPSNRPVHQSLIETVGITKMGV 359

Query: 944  YIEASSGFSQSNDSIHCHHGILSAEIGQLSTIPPKQRSIEAVKAYVKNKRDVPVEAFKGG 765
            YIEASSGFSQSNDSIHCHHGI+SAEIGQLSTIPPK+RS EAV+ ++KNK+D+PVE FKGG
Sbjct: 360  YIEASSGFSQSNDSIHCHHGIMSAEIGQLSTIPPKKRSPEAVQEFIKNKKDLPVELFKGG 419

Query: 764  FILSKVASAWSVGELRLVNTNVDDNPAVTFNYFSHPYDLQRCVEGIRLSTKVVQSQHFTN 585
            FILSKVA+ WSVGELRL NTNV+DNP VTFNYFSHPYDL RCV+GIRL+ KV QS+HFTN
Sbjct: 420  FILSKVANPWSVGELRLNNTNVNDNPVVTFNYFSHPYDLHRCVKGIRLAIKVAQSKHFTN 479

Query: 584  YTLCNRETTEKLLNLSVKANVNFIPKHANDTKSLEQFCRDTVITIWHYHGGCHVGKVVSP 405
            YTLC+++T+E+LLNL+VKANVNFI KH NDT S+ QFC+DTVITIWHYHGGCH+GKVVSP
Sbjct: 480  YTLCDKKTSEELLNLTVKANVNFITKHPNDTASIAQFCKDTVITIWHYHGGCHLGKVVSP 539

Query: 404  DYKVLGIDRLRVVDGSTFTESPGTNPQATVMMMGRYMGVKI 282
            DYKVLG+DRLRVVDGSTF ESPGTNPQATVMMMGRYMG+KI
Sbjct: 540  DYKVLGVDRLRVVDGSTFDESPGTNPQATVMMMGRYMGLKI 580


>ref|XP_003531615.1| PREDICTED: protein HOTHEAD-like [Glycine max]
          Length = 591

 Score =  942 bits (2435), Expect = 0.0
 Identities = 460/558 (82%), Positives = 506/558 (90%)
 Frame = -2

Query: 1955 PPCQGKQKWHEDHQYXXXXXXXXXXXXXXXXXSLNKAYDYIIVGGGTAGCPLAATLSQNF 1776
            P  QG Q WHE+ +Y                 + N AYDYIIVGGGTAGCPLAATLSQNF
Sbjct: 24   PLSQGNQHWHEN-RYPFIRNASSFSSPSISTTTSNNAYDYIIVGGGTAGCPLAATLSQNF 82

Query: 1775 SVLLLERGGVPFTNPNVTFLENFHITLADTSPTSASQYFISTDGVFNARARVLGGGSSIN 1596
            SVL+LERGGVPFTNPNV+FLENFHITLAD SPTSASQYFISTDGV+N+RARVLGGGSSIN
Sbjct: 83   SVLVLERGGVPFTNPNVSFLENFHITLADISPTSASQYFISTDGVYNSRARVLGGGSSIN 142

Query: 1595 AGFYTRASQRFVEKAGWDAKLVNESYPWVEKQIVHRPKFSAYQRAVRESLVDSGVSPFNG 1416
            AGFYTRA+ RF++K GWDAKLVNESYPWVEKQIVHRPKFS YQRA R+SL+DSGVSPFNG
Sbjct: 143  AGFYTRANPRFIKKVGWDAKLVNESYPWVEKQIVHRPKFSPYQRAFRDSLLDSGVSPFNG 202

Query: 1415 FTYDHIYGTKVGGTIFDRFGRRHTSAELLASGNPDKLTVLVHATVQKIVFDTTGKRSKAV 1236
            FTYDH+YGTKVGGTIFDRFGRRHT+AELLASGN DKLTVLV ATVQKIVFD  GKR KAV
Sbjct: 203  FTYDHLYGTKVGGTIFDRFGRRHTAAELLASGNHDKLTVLVCATVQKIVFDRKGKRPKAV 262

Query: 1235 GVIFKDENGKQHEAVLGNDIHSEVIVSSGAIGTPQLLLLSGIGPKAELKNLNISVVLDNP 1056
            GVIF+DE+GKQHEA+L ND HSEVI+SSGAIGTPQLL+LSGIGPKAEL+ L+I VVLDN 
Sbjct: 263  GVIFQDEHGKQHEAILSNDKHSEVIMSSGAIGTPQLLMLSGIGPKAELQKLSIPVVLDNH 322

Query: 1055 FVGKGMIDNPMNTIFVPSNRPVVQSLIQTVGITKLGVYIEASSGFSQSNDSIHCHHGILS 876
            FVGKGM+DNPMNT+FVPSNRPV QSLI+TVGITK+GVYIEASSGFSQSNDSIHCHHGI+S
Sbjct: 323  FVGKGMVDNPMNTMFVPSNRPVNQSLIETVGITKMGVYIEASSGFSQSNDSIHCHHGIMS 382

Query: 875  AEIGQLSTIPPKQRSIEAVKAYVKNKRDVPVEAFKGGFILSKVASAWSVGELRLVNTNVD 696
            AEIGQLSTIPPK+RS EAV+ ++KNK+D+PVE FKGGFILSKVA+ WSVGELRL NTNV+
Sbjct: 383  AEIGQLSTIPPKERSPEAVQEFIKNKKDIPVELFKGGFILSKVANPWSVGELRLNNTNVN 442

Query: 695  DNPAVTFNYFSHPYDLQRCVEGIRLSTKVVQSQHFTNYTLCNRETTEKLLNLSVKANVNF 516
            DNP VTFNYFSHPYDL RCV+GIRL+ KVVQS+HFTNYTLC+++TTE+LLNL+VKANVNF
Sbjct: 443  DNPVVTFNYFSHPYDLHRCVKGIRLAIKVVQSKHFTNYTLCDKKTTEELLNLTVKANVNF 502

Query: 515  IPKHANDTKSLEQFCRDTVITIWHYHGGCHVGKVVSPDYKVLGIDRLRVVDGSTFTESPG 336
            IPKH NDT S+ QFC+DTVITIWHYHGGCHVGKVVSPDYKVLG+DRLRVVDGSTF ESPG
Sbjct: 503  IPKHPNDTASIAQFCKDTVITIWHYHGGCHVGKVVSPDYKVLGVDRLRVVDGSTFDESPG 562

Query: 335  TNPQATVMMMGRYMGVKI 282
            TNPQATVMMMGRYMG+KI
Sbjct: 563  TNPQATVMMMGRYMGLKI 580


>ref|XP_003597604.1| Protein HOTHEAD [Medicago truncatula] gi|355486652|gb|AES67855.1|
            Protein HOTHEAD [Medicago truncatula]
          Length = 594

 Score =  922 bits (2382), Expect = 0.0
 Identities = 452/583 (77%), Positives = 507/583 (86%), Gaps = 3/583 (0%)
 Frame = -2

Query: 2021 MASVGAVKXXXXXXXXXXXXXLPPCQG--KQKWHEDHQYXXXXXXXXXXXXXXXXXS-LN 1851
            MA++GAVK             LP CQG  KQ  +  ++Y                 +  N
Sbjct: 1    MATLGAVKFLLCLLMIWLINFLPLCQGLGKQNLNNGYKYPFIRKASSFSSPPSISTTNTN 60

Query: 1850 KAYDYIIVGGGTAGCPLAATLSQNFSVLLLERGGVPFTNPNVTFLENFHITLADTSPTSA 1671
              YDYIIVGGGTAGCPLAATLSQNF+VLLLERGGVPFTNPNV+FL+NFHITLAD SPTSA
Sbjct: 61   DGYDYIIVGGGTAGCPLAATLSQNFNVLLLERGGVPFTNPNVSFLDNFHITLADISPTSA 120

Query: 1670 SQYFISTDGVFNARARVLGGGSSINAGFYTRASQRFVEKAGWDAKLVNESYPWVEKQIVH 1491
            SQ+F+STDGVFNARARVLGGGSSINAGFYTRAS RF++K GWD KLVN+SYPWVEKQIVH
Sbjct: 121  SQFFLSTDGVFNARARVLGGGSSINAGFYTRASSRFIQKMGWDTKLVNKSYPWVEKQIVH 180

Query: 1490 RPKFSAYQRAVRESLVDSGVSPFNGFTYDHIYGTKVGGTIFDRFGRRHTSAELLASGNPD 1311
            RP FS +QRA R+ L+D+GVSPFNGFTY+H YGTKVGGTIFDRFGRRHT+AELL+SGNP+
Sbjct: 181  RPTFSHWQRAFRDGLLDAGVSPFNGFTYEHKYGTKVGGTIFDRFGRRHTAAELLSSGNPN 240

Query: 1310 KLTVLVHATVQKIVFDTTGKRSKAVGVIFKDENGKQHEAVLGNDIHSEVIVSSGAIGTPQ 1131
            KLTVL++ATVQKIVFDTTGKR KA+GVIFKDENGKQH+A+LGND  SEVIVSSGAIGTPQ
Sbjct: 241  KLTVLIYATVQKIVFDTTGKRPKAMGVIFKDENGKQHKAILGNDRESEVIVSSGAIGTPQ 300

Query: 1130 LLLLSGIGPKAELKNLNISVVLDNPFVGKGMIDNPMNTIFVPSNRPVVQSLIQTVGITKL 951
            +LLLSGIGPKAEL+NL I VVLDN FVGKGM DNPMNTIFVP  + V QSLI+TVGIT  
Sbjct: 301  MLLLSGIGPKAELENLKIPVVLDNRFVGKGMADNPMNTIFVPLKKSVKQSLIETVGITNK 360

Query: 950  GVYIEASSGFSQSNDSIHCHHGILSAEIGQLSTIPPKQRSIEAVKAYVKNKRDVPVEAFK 771
            GVYIEAS GF Q+NDSIHCHHG+LSAEIGQLSTIPPKQRS E++KA+VKNK+D+P+EAFK
Sbjct: 361  GVYIEASCGFGQTNDSIHCHHGLLSAEIGQLSTIPPKQRSAESIKAFVKNKKDIPIEAFK 420

Query: 770  GGFILSKVASAWSVGELRLVNTNVDDNPAVTFNYFSHPYDLQRCVEGIRLSTKVVQSQHF 591
            GGFILSKVA+ WS G+L+L+NTNVDDNPAVTFNYFSHPYDL RCVEGIRL+TKVVQSQHF
Sbjct: 421  GGFILSKVANPWSTGDLKLINTNVDDNPAVTFNYFSHPYDLHRCVEGIRLATKVVQSQHF 480

Query: 590  TNYTLCNRETTEKLLNLSVKANVNFIPKHANDTKSLEQFCRDTVITIWHYHGGCHVGKVV 411
            TN TLC ++TTE+LLN +VKAN+N IPKH NDT+SLEQFCRDTVITIWHYHGGCHVGKV+
Sbjct: 481  TNLTLCEKQTTEQLLNNTVKANINLIPKHVNDTESLEQFCRDTVITIWHYHGGCHVGKVI 540

Query: 410  SPDYKVLGIDRLRVVDGSTFTESPGTNPQATVMMMGRYMGVKI 282
              D+KVLG++RLRV+DGSTFTESPGTNPQATVMMMGRYMGVKI
Sbjct: 541  DSDHKVLGVNRLRVIDGSTFTESPGTNPQATVMMMGRYMGVKI 583


>ref|XP_003546687.1| PREDICTED: protein HOTHEAD-like [Glycine max]
          Length = 585

 Score =  917 bits (2370), Expect = 0.0
 Identities = 458/581 (78%), Positives = 493/581 (84%), Gaps = 1/581 (0%)
 Frame = -2

Query: 2021 MASVGAVKXXXXXXXXXXXXXLPPC-QGKQKWHEDHQYXXXXXXXXXXXXXXXXXSLNKA 1845
            MA +GAVK               P  QGKQ W+E + +                   +  
Sbjct: 1    MAFIGAVKFLLQYLVLLCLLNYLPLSQGKQNWNEGYPFIRRASSLGYNGK-------SGG 53

Query: 1844 YDYIIVGGGTAGCPLAATLSQNFSVLLLERGGVPFTNPNVTFLENFHITLADTSPTSASQ 1665
            YDYIIVGGGTAGCPLAATLSQ F VLLLERGGVPF NPNV+FL NFHITLADTSPTSASQ
Sbjct: 54   YDYIIVGGGTAGCPLAATLSQKFKVLLLERGGVPFNNPNVSFLHNFHITLADTSPTSASQ 113

Query: 1664 YFISTDGVFNARARVLGGGSSINAGFYTRASQRFVEKAGWDAKLVNESYPWVEKQIVHRP 1485
            YFISTDGV NAR RVLGGGSSINAGFYTRA  RF+ K GWD KLVNESYPWVEKQIVHRP
Sbjct: 114  YFISTDGVLNARGRVLGGGSSINAGFYTRADPRFIRKVGWDTKLVNESYPWVEKQIVHRP 173

Query: 1484 KFSAYQRAVRESLVDSGVSPFNGFTYDHIYGTKVGGTIFDRFGRRHTSAELLASGNPDKL 1305
            KFS +QRAVR+ L+ +GVSPFNGFTYDH YGTKVGGTIFDRFGRRHT+AELLAS NP KL
Sbjct: 174  KFSDWQRAVRDGLLAAGVSPFNGFTYDHKYGTKVGGTIFDRFGRRHTAAELLASANPHKL 233

Query: 1304 TVLVHATVQKIVFDTTGKRSKAVGVIFKDENGKQHEAVLGNDIHSEVIVSSGAIGTPQLL 1125
            TVL+HATVQ IVFDTTGKR KA GVIFKDENGKQHEA LGND  SEVIVSSGA+GTPQLL
Sbjct: 234  TVLIHATVQNIVFDTTGKRPKATGVIFKDENGKQHEAYLGNDRQSEVIVSSGALGTPQLL 293

Query: 1124 LLSGIGPKAELKNLNISVVLDNPFVGKGMIDNPMNTIFVPSNRPVVQSLIQTVGITKLGV 945
            LLSGIGPKAEL+ LNI VVLDN FVGKGM DNPMNTIFVPS R V QSLI+TVGIT LGV
Sbjct: 294  LLSGIGPKAELQKLNIPVVLDNQFVGKGMADNPMNTIFVPSKRSVQQSLIETVGITNLGV 353

Query: 944  YIEASSGFSQSNDSIHCHHGILSAEIGQLSTIPPKQRSIEAVKAYVKNKRDVPVEAFKGG 765
            YIE SSGF QS DSIHCHHGILSAEIGQLSTIPPKQRS EAVKAYVK+KRD+PVEAF+GG
Sbjct: 354  YIETSSGFGQSKDSIHCHHGILSAEIGQLSTIPPKQRSQEAVKAYVKSKRDIPVEAFRGG 413

Query: 764  FILSKVASAWSVGELRLVNTNVDDNPAVTFNYFSHPYDLQRCVEGIRLSTKVVQSQHFTN 585
            FILSKVA+ WS GEL+L NTNV+DNPAVTFNYFSHPYDL+RCVEGIRL+ KVVQS+H TN
Sbjct: 414  FILSKVANPWSTGELKLKNTNVEDNPAVTFNYFSHPYDLRRCVEGIRLAIKVVQSEHVTN 473

Query: 584  YTLCNRETTEKLLNLSVKANVNFIPKHANDTKSLEQFCRDTVITIWHYHGGCHVGKVVSP 405
            YTLC RET EK+LNLSVKAN+N IPK  NDTKS+EQFCRD+VITIWHYHGGCHVGKVV+ 
Sbjct: 474  YTLCERETAEKMLNLSVKANINLIPKRPNDTKSVEQFCRDSVITIWHYHGGCHVGKVVNS 533

Query: 404  DYKVLGIDRLRVVDGSTFTESPGTNPQATVMMMGRYMGVKI 282
            ++KVLG+DRLRVVDGSTF+ESPGTNPQATVMMMGRYMG+KI
Sbjct: 534  EHKVLGVDRLRVVDGSTFSESPGTNPQATVMMMGRYMGLKI 574


>ref|XP_003543593.1| PREDICTED: protein HOTHEAD-like [Glycine max]
          Length = 585

 Score =  915 bits (2366), Expect = 0.0
 Identities = 454/581 (78%), Positives = 494/581 (85%), Gaps = 1/581 (0%)
 Frame = -2

Query: 2021 MASVGAVKXXXXXXXXXXXXXLPP-CQGKQKWHEDHQYXXXXXXXXXXXXXXXXXSLNKA 1845
            MA +GAVK               P  QGKQ W+E + +                   +  
Sbjct: 1    MAFIGAVKFLLQHLLLLCLFNYLPFSQGKQNWNEGYPFIRRASSLGYNGK-------SGG 53

Query: 1844 YDYIIVGGGTAGCPLAATLSQNFSVLLLERGGVPFTNPNVTFLENFHITLADTSPTSASQ 1665
            YDYIIVGGGTAGCPLAATLS+ F VL+LERGGVPF NPNV+FL NFHITLADTSPTSASQ
Sbjct: 54   YDYIIVGGGTAGCPLAATLSKKFKVLVLERGGVPFNNPNVSFLHNFHITLADTSPTSASQ 113

Query: 1664 YFISTDGVFNARARVLGGGSSINAGFYTRASQRFVEKAGWDAKLVNESYPWVEKQIVHRP 1485
            YFISTDGV NAR RVLGG +SINAGFYTRA  RF+ K GWD KLVNESYPWVEKQIVHRP
Sbjct: 114  YFISTDGVLNARGRVLGGATSINAGFYTRADPRFIRKVGWDTKLVNESYPWVEKQIVHRP 173

Query: 1484 KFSAYQRAVRESLVDSGVSPFNGFTYDHIYGTKVGGTIFDRFGRRHTSAELLASGNPDKL 1305
            KFS +QRAVR+ L+ +GVSPFNGFTYDH YGTKVGGTIFDRFGRRHT+AELLAS NP KL
Sbjct: 174  KFSDWQRAVRDGLLAAGVSPFNGFTYDHKYGTKVGGTIFDRFGRRHTAAELLASANPHKL 233

Query: 1304 TVLVHATVQKIVFDTTGKRSKAVGVIFKDENGKQHEAVLGNDIHSEVIVSSGAIGTPQLL 1125
            TVL+HATVQKIVFDT GKR KA GVIFKDENGKQHEA LGND  SEVIVSSGA+GTPQLL
Sbjct: 234  TVLIHATVQKIVFDTKGKRPKATGVIFKDENGKQHEAYLGNDRQSEVIVSSGALGTPQLL 293

Query: 1124 LLSGIGPKAELKNLNISVVLDNPFVGKGMIDNPMNTIFVPSNRPVVQSLIQTVGITKLGV 945
            LLSGIGPKAEL+ LNI VVLDN FVGKGM DNPMNTIFVPS RPV QSLI+TVGIT LGV
Sbjct: 294  LLSGIGPKAELQKLNIPVVLDNQFVGKGMADNPMNTIFVPSKRPVQQSLIETVGITNLGV 353

Query: 944  YIEASSGFSQSNDSIHCHHGILSAEIGQLSTIPPKQRSIEAVKAYVKNKRDVPVEAFKGG 765
            YIE SSGF QS DSIHCHHGILSAEIGQLSTIPPKQRS EAVKAYVK+KRD+PVEAF+GG
Sbjct: 354  YIETSSGFGQSKDSIHCHHGILSAEIGQLSTIPPKQRSREAVKAYVKSKRDIPVEAFRGG 413

Query: 764  FILSKVASAWSVGELRLVNTNVDDNPAVTFNYFSHPYDLQRCVEGIRLSTKVVQSQHFTN 585
            FILSKVA+ WS GEL+L+NTNV+DNPAVTFNYFSHPYDL+RCVEGIRL+ KVVQ++H TN
Sbjct: 414  FILSKVANPWSTGELKLINTNVEDNPAVTFNYFSHPYDLKRCVEGIRLAIKVVQTEHVTN 473

Query: 584  YTLCNRETTEKLLNLSVKANVNFIPKHANDTKSLEQFCRDTVITIWHYHGGCHVGKVVSP 405
            YTLC RE  EK+LNLSVKAN+N IPKH NDTKS+EQFCRD+VITIWHYHGGCHVGKVV+ 
Sbjct: 474  YTLCERENAEKMLNLSVKANINLIPKHPNDTKSVEQFCRDSVITIWHYHGGCHVGKVVNS 533

Query: 404  DYKVLGIDRLRVVDGSTFTESPGTNPQATVMMMGRYMGVKI 282
            ++KVLG+DRLRVVDGSTF+ESPGTNPQATVMMMGRYMG+KI
Sbjct: 534  EHKVLGVDRLRVVDGSTFSESPGTNPQATVMMMGRYMGLKI 574