BLASTX nr result
ID: Glycyrrhiza24_contig00005457
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Glycyrrhiza24_contig00005457
(1881 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q84KK6.1|I4OMT_GLYEC RecName: Full=Isoflavone 4'-O-methyltran... 572 0.0
sp|Q29U70.1|I4OMT_MEDTR RecName: Full=Isoflavone 4'-O-methyltran... 500 e-163
sp|Q84KK4.1|I4OMT_LOTJA RecName: Full=Isoflavone 4'-O-methyltran... 486 e-162
sp|O24305.1|M3OM1_PEA RecName: Full=6a-hydroxymaackiain methyltr... 493 e-160
pdb|1ZG3|A Chain A, Crystal Structure Of The Isoflavanone 4'-O-M... 491 e-160
>sp|Q84KK6.1|I4OMT_GLYEC RecName: Full=Isoflavone 4'-O-methyltransferase; Short=GeHI4'OMT;
AltName: Full=2,7,4'-trihydroxyisoflavanone
4'-O-methyltransferase; AltName:
Full=S-adenosyl-L-methionine:2,7,
4'-trihydroxyisoflavanone 4'-O-methyltransferase
gi|28804592|dbj|BAC58011.1| S-adenosyl-L-methionine:
2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase
[Glycyrrhiza echinata]
Length = 367
Score = 572 bits (1474), Expect(2) = 0.0
Identities = 287/302 (95%), Positives = 290/302 (96%), Gaps = 1/302 (0%)
Frame = -2
Query: 1700 MDFSTNGSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIADVIHNHGKPITLPQLASA 1521
M FSTNGSEE ELYHAQIHLYKHVYNFVSSMALKSAMELGIADVIHNHGKPITLP+LASA
Sbjct: 1 MAFSTNGSEEIELYHAQIHLYKHVYNFVSSMALKSAMELGIADVIHNHGKPITLPELASA 60
Query: 1520 LKLHPSKVGVLYRFLRLLTHNGFFAKTTVPSQNGKEGEEEE-TAYALTPPSKLLVKGKPT 1344
LKLHPSKVG+LYRFLRLLTHNGFFAKTTVPSQNGK+GEEEE TAYALTPPSKLLVKGKPT
Sbjct: 61 LKLHPSKVGILYRFLRLLTHNGFFAKTTVPSQNGKDGEEEEETAYALTPPSKLLVKGKPT 120
Query: 1343 CLASIVRGALHPSSLDMWRSSEKWFNEDKELTLFESATGESFWDFLNKDSESGTLSMFQE 1164
CLASIVRGALHPSSLDMWRSSEKWF EDKELTLFESATGESFWDFLNKDSESGTLSMFQE
Sbjct: 121 CLASIVRGALHPSSLDMWRSSEKWFKEDKELTLFESATGESFWDFLNKDSESGTLSMFQE 180
Query: 1163 AMAADSQMFKLALKECRHVFEGLESLVDXXXXXXXXTKLIHEEFPHLKCTVFDQPQVVGN 984
AMAADSQMFKLALKECRHVFEGLESLVD TKLIHEEFPHLKCTVFDQPQVVGN
Sbjct: 181 AMAADSQMFKLALKECRHVFEGLESLVDVGGGTGGVTKLIHEEFPHLKCTVFDQPQVVGN 240
Query: 983 LSGNENLKFVGGDMFKSIPPADAVLLKWVLHDWNDELSLKILKNSKEAISGKGKEGKVII 804
LSGNENLKFVGGDMFKSIPPADAVLLKWVLHDWNDELSLKILKNSKEAISGKGKEGKVII
Sbjct: 241 LSGNENLKFVGGDMFKSIPPADAVLLKWVLHDWNDELSLKILKNSKEAISGKGKEGKVII 300
Query: 803 ID 798
ID
Sbjct: 301 ID 302
Score = 123 bits (309), Expect(2) = 0.0
Identities = 63/71 (88%), Positives = 63/71 (88%)
Frame = -3
Query: 745 KVXXXXXXXDEASGDRELTELQLDYDLVMLTMFNGKEREKKEWEKLISDAGFSSYKITSI 566
KV DEASGDRELTELQLDYDLVMLTMFNGKEREKKEWEKLISDAGFSSYKIT I
Sbjct: 297 KVIIIDISIDEASGDRELTELQLDYDLVMLTMFNGKEREKKEWEKLISDAGFSSYKITPI 356
Query: 565 CGFKSLIEVFP 533
CGFKSLIEVFP
Sbjct: 357 CGFKSLIEVFP 367
>sp|Q29U70.1|I4OMT_MEDTR RecName: Full=Isoflavone 4'-O-methyltransferase; Short=MtHI4'OMT;
AltName: Full=2,7,4'-trihydroxyisoflavanone
4'-O-methyltransferase gi|62871343|gb|AAY18581.1| SAM
dependent isoflavanone
4'-O-methyltransferase/(+)-6a-hydroxymaackiain-3-0-
methyltransferase [Medicago truncatula]
Length = 364
Score = 500 bits (1288), Expect(2) = e-163
Identities = 253/302 (83%), Positives = 270/302 (89%), Gaps = 1/302 (0%)
Frame = -2
Query: 1700 MDFSTNGSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIADVIHNHGKPITLPQLASA 1521
M FSTNGSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIAD IHNHGKP+TL +LAS+
Sbjct: 1 MAFSTNGSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIADAIHNHGKPMTLSELASS 60
Query: 1520 LKLHPSKVGVLYRFLRLLTHNGFFAKTTVPSQNGKEG-EEEETAYALTPPSKLLVKGKPT 1344
LKLHPSKV +L+RFLRLLTHNGFFAKT V GKEG EEEE AY+LTPPSKLL+ GKPT
Sbjct: 61 LKLHPSKVNILHRFLRLLTHNGFFAKTIV---KGKEGDEEEEIAYSLTPPSKLLISGKPT 117
Query: 1343 CLASIVRGALHPSSLDMWRSSEKWFNEDKELTLFESATGESFWDFLNKDSESGTLSMFQE 1164
CL+SIV+GALHPSSLDMW SS+KWFNEDKE TLFE ATGESFWDFLNKDSES TLSMFQ+
Sbjct: 118 CLSSIVKGALHPSSLDMWSSSKKWFNEDKEQTLFECATGESFWDFLNKDSESSTLSMFQD 177
Query: 1163 AMAADSQMFKLALKECRHVFEGLESLVDXXXXXXXXTKLIHEEFPHLKCTVFDQPQVVGN 984
AMA+DS+MFKL L+E + VFEGLESLVD TKLIHE FPHLKCTVFDQPQVVGN
Sbjct: 178 AMASDSRMFKLVLQENKRVFEGLESLVDVGGGTGGVTKLIHEIFPHLKCTVFDQPQVVGN 237
Query: 983 LSGNENLKFVGGDMFKSIPPADAVLLKWVLHDWNDELSLKILKNSKEAISGKGKEGKVII 804
L+GNENL FVGGDMFKSIP ADAVLLKWVLHDWNDE SLKILKNSKEAIS KGK+GKVII
Sbjct: 238 LTGNENLNFVGGDMFKSIPSADAVLLKWVLHDWNDEQSLKILKNSKEAISHKGKDGKVII 297
Query: 803 ID 798
ID
Sbjct: 298 ID 299
Score = 102 bits (253), Expect(2) = e-163
Identities = 54/71 (76%), Positives = 56/71 (78%)
Frame = -3
Query: 745 KVXXXXXXXDEASGDRELTELQLDYDLVMLTMFNGKEREKKEWEKLISDAGFSSYKITSI 566
KV DE S DR LTELQLDYDLVMLTMF GKER K+EWEKLI DAGFSSYKIT I
Sbjct: 294 KVIIIDISIDETSDDRGLTELQLDYDLVMLTMFLGKERTKQEWEKLIYDAGFSSYKITPI 353
Query: 565 CGFKSLIEVFP 533
GFKSLIEV+P
Sbjct: 354 SGFKSLIEVYP 364
>sp|Q84KK4.1|I4OMT_LOTJA RecName: Full=Isoflavone 4'-O-methyltransferase; Short=LjHI4'OMT;
AltName: Full=2,7,4'-trihydroxyisoflavanone
4'-O-methyltransferase; AltName:
Full=S-adenosyl-L-methionine:
2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase
gi|28804596|dbj|BAC58013.1| S-adenosyl-L-methionine:
2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase
[Lotus japonicus] gi|388490958|gb|AFK33545.1| unknown
[Lotus japonicus]
Length = 365
Score = 486 bits (1250), Expect(2) = e-162
Identities = 248/303 (81%), Positives = 269/303 (88%), Gaps = 2/303 (0%)
Frame = -2
Query: 1700 MDFST-NGSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIADVIHNHGKPITLPQLAS 1524
MDFS+ NGSE++EL AQIHLYKHVYNFVSSMALKSAMELGIADVIH+HGKPITLP+LA+
Sbjct: 1 MDFSSSNGSEDTELSQAQIHLYKHVYNFVSSMALKSAMELGIADVIHSHGKPITLPELAT 60
Query: 1523 ALKLHPSKVGVLYRFLRLLTHNGFFAKTTVPSQNGKEGEEEETAYALTPPSKLLVKGKPT 1344
AL L PSK+GVL+RFLRLLTHNGFFAKTTV + EG EEETAY LTPPSKLLVK T
Sbjct: 61 ALNLRPSKIGVLHRFLRLLTHNGFFAKTTV---SRGEGAEEETAYGLTPPSKLLVKSNST 117
Query: 1343 CLASIVRGALHPSSLDMWRSSEKWFNED-KELTLFESATGESFWDFLNKDSESGTLSMFQ 1167
CLA IV+GALHPSSLDMWRSS+KWF ED +ELTLFESATGESFW+FLNK++ES TLSMFQ
Sbjct: 118 CLAPIVKGALHPSSLDMWRSSKKWFLEDNEELTLFESATGESFWEFLNKETESDTLSMFQ 177
Query: 1166 EAMAADSQMFKLALKECRHVFEGLESLVDXXXXXXXXTKLIHEEFPHLKCTVFDQPQVVG 987
EAMAADS MFKLALKEC+HVFEGL SLVD TKLI E FPH+KCTVFDQPQVV
Sbjct: 178 EAMAADSHMFKLALKECKHVFEGLGSLVDVAGGRGGVTKLIREAFPHVKCTVFDQPQVVA 237
Query: 986 NLSGNENLKFVGGDMFKSIPPADAVLLKWVLHDWNDELSLKILKNSKEAISGKGKEGKVI 807
NL+G+ENL FVGGDMFKS+PPADAVLLKWVLHDWNDELSLKILKN KEAISG+GKEGKVI
Sbjct: 238 NLTGDENLNFVGGDMFKSVPPADAVLLKWVLHDWNDELSLKILKNCKEAISGRGKEGKVI 297
Query: 806 IID 798
IID
Sbjct: 298 IID 300
Score = 115 bits (288), Expect(2) = e-162
Identities = 59/71 (83%), Positives = 60/71 (84%)
Frame = -3
Query: 745 KVXXXXXXXDEASGDRELTELQLDYDLVMLTMFNGKEREKKEWEKLISDAGFSSYKITSI 566
KV DE S DRELTEL+LDYDLVMLTMFNGKEREKKEWEKLI DAGFSSYKIT I
Sbjct: 295 KVIIIDISIDETSDDRELTELKLDYDLVMLTMFNGKEREKKEWEKLIYDAGFSSYKITPI 354
Query: 565 CGFKSLIEVFP 533
CGFKSLIEVFP
Sbjct: 355 CGFKSLIEVFP 365
>sp|O24305.1|M3OM1_PEA RecName: Full=6a-hydroxymaackiain methyltransferase 1; AltName:
Full=Isoflavone 4'-O-methyltransferase
gi|1568637|gb|AAC49856.1| 6a-hydroxymaackiain
methyltransferase [Pisum sativum]
Length = 360
Score = 493 bits (1269), Expect(2) = e-160
Identities = 245/301 (81%), Positives = 268/301 (89%)
Frame = -2
Query: 1700 MDFSTNGSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIADVIHNHGKPITLPQLASA 1521
MDFSTNGSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIAD IHNHGKP+TLP+L+S+
Sbjct: 1 MDFSTNGSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIADAIHNHGKPMTLPELSSS 60
Query: 1520 LKLHPSKVGVLYRFLRLLTHNGFFAKTTVPSQNGKEGEEEETAYALTPPSKLLVKGKPTC 1341
LKLHPSKV +LYRFLRLLTHNGFFAKTTV S G EEETAY LTP SKLLV GK TC
Sbjct: 61 LKLHPSKVNILYRFLRLLTHNGFFAKTTVKSNEG----EEETAYVLTPSSKLLVSGKSTC 116
Query: 1340 LASIVRGALHPSSLDMWRSSEKWFNEDKELTLFESATGESFWDFLNKDSESGTLSMFQEA 1161
L+S+V+GALHPSSLDMW S+KWF+EDKE TLFE ATGE++WDFLNKDS+S LSMFQ+A
Sbjct: 117 LSSLVKGALHPSSLDMWGVSKKWFHEDKEQTLFECATGENYWDFLNKDSDS--LSMFQDA 174
Query: 1160 MAADSQMFKLALKECRHVFEGLESLVDXXXXXXXXTKLIHEEFPHLKCTVFDQPQVVGNL 981
MAADS++FKLA++E +HVFEGLESLVD KLIHE FPH+KCTVFDQPQVVGNL
Sbjct: 175 MAADSRLFKLAIQENKHVFEGLESLVDVAGGTGGVAKLIHEAFPHIKCTVFDQPQVVGNL 234
Query: 980 SGNENLKFVGGDMFKSIPPADAVLLKWVLHDWNDELSLKILKNSKEAISGKGKEGKVIII 801
+GNENL FVGGDMFKS+P ADAVLLKWVLHDWNDELSLKILKNSKEAIS KGK+GKVIII
Sbjct: 235 TGNENLNFVGGDMFKSVPSADAVLLKWVLHDWNDELSLKILKNSKEAISHKGKDGKVIII 294
Query: 800 D 798
D
Sbjct: 295 D 295
Score = 102 bits (253), Expect(2) = e-160
Identities = 53/71 (74%), Positives = 56/71 (78%)
Frame = -3
Query: 745 KVXXXXXXXDEASGDRELTELQLDYDLVMLTMFNGKEREKKEWEKLISDAGFSSYKITSI 566
KV DE S DR LTELQL+YD+VMLTMF GKER KKEWEKLI DAGFS YKIT I
Sbjct: 290 KVIIIDISIDENSDDRGLTELQLEYDVVMLTMFLGKERTKKEWEKLIYDAGFSRYKITPI 349
Query: 565 CGFKSLIEVFP 533
CGFKSLIEV+P
Sbjct: 350 CGFKSLIEVYP 360
>pdb|1ZG3|A Chain A, Crystal Structure Of The Isoflavanone 4'-O-Methyltransferase
Complexed With Sah And 2,7,4'-Trihydroxyisoflavanone
Length = 358
Score = 491 bits (1264), Expect(2) = e-160
Identities = 248/296 (83%), Positives = 265/296 (89%), Gaps = 1/296 (0%)
Frame = -2
Query: 1682 GSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIADVIHNHGKPITLPQLASALKLHPS 1503
GSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIAD IHNHGKP+TL +LAS+LKLHPS
Sbjct: 1 GSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIADAIHNHGKPMTLSELASSLKLHPS 60
Query: 1502 KVGVLYRFLRLLTHNGFFAKTTVPSQNGKEG-EEEETAYALTPPSKLLVKGKPTCLASIV 1326
KV +L+RFLRLLTHNGFFAKT V GKEG EEEE AY+LTPPSKLL+ GKPTCL+SIV
Sbjct: 61 KVNILHRFLRLLTHNGFFAKTIV---KGKEGDEEEEIAYSLTPPSKLLISGKPTCLSSIV 117
Query: 1325 RGALHPSSLDMWRSSEKWFNEDKELTLFESATGESFWDFLNKDSESGTLSMFQEAMAADS 1146
+GALHPSSLDMW SS+KWFNEDKE TLFE ATGESFWDFLNKDSES TLSMFQ+AMA+DS
Sbjct: 118 KGALHPSSLDMWSSSKKWFNEDKEQTLFECATGESFWDFLNKDSESSTLSMFQDAMASDS 177
Query: 1145 QMFKLALKECRHVFEGLESLVDXXXXXXXXTKLIHEEFPHLKCTVFDQPQVVGNLSGNEN 966
+MFKL L+E + VFEGLESLVD TKLIHE FPHLKCTVFDQPQVVGNL+GNEN
Sbjct: 178 RMFKLVLQENKRVFEGLESLVDVGGGTGGVTKLIHEIFPHLKCTVFDQPQVVGNLTGNEN 237
Query: 965 LKFVGGDMFKSIPPADAVLLKWVLHDWNDELSLKILKNSKEAISGKGKEGKVIIID 798
L FVGGDMFKSIP ADAVLLKWVLHDWNDE SLKILKNSKEAIS KGK+GKVIIID
Sbjct: 238 LNFVGGDMFKSIPSADAVLLKWVLHDWNDEQSLKILKNSKEAISHKGKDGKVIIID 293
Score = 102 bits (253), Expect(2) = e-160
Identities = 54/71 (76%), Positives = 56/71 (78%)
Frame = -3
Query: 745 KVXXXXXXXDEASGDRELTELQLDYDLVMLTMFNGKEREKKEWEKLISDAGFSSYKITSI 566
KV DE S DR LTELQLDYDLVMLTMF GKER K+EWEKLI DAGFSSYKIT I
Sbjct: 288 KVIIIDISIDETSDDRGLTELQLDYDLVMLTMFLGKERTKQEWEKLIYDAGFSSYKITPI 347
Query: 565 CGFKSLIEVFP 533
GFKSLIEV+P
Sbjct: 348 SGFKSLIEVYP 358