BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Glycyrrhiza24_contig00004060
         (1502 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFA89978.1| isoflavone-3'-hydroxylase [Astragalus membranaceus]    635   e-180
gb|AAQ20041.1| isoflavone 3'-hydroxylase, partial [Medicago trun...   618   e-174
gb|AAO31712.1| cytochrome P450 [Pisum sativum]                        553   e-155
ref|XP_003532671.1| PREDICTED: isoflavone 2'-hydroxylase-like [G...   547   e-153
ref|XP_003548043.1| PREDICTED: isoflavone 2'-hydroxylase-like [G...   530   e-148

>gb|AFA89978.1| isoflavone-3'-hydroxylase [Astragalus membranaceus]
          Length = 505

 Score =  635 bits (1639), Expect = e-180
 Identities = 320/438 (73%), Positives = 350/438 (79%), Gaps = 1/438 (0%)
 Frame = -1

Query: 1502 SLWFGXXXXXXXXXXXXVQECFNRNDVVLANRPKFLTGKYVFYNYTTLGSAPYGDHWRNL 1323
            SLWFG             QECF +NDVVLANRPKFLTGKY+FYNYTTLGSA YGDHWRNL
Sbjct: 68   SLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNL 127

Query: 1322 RRITTIDVLSNHRLNSFVGIRSDETMRFVQKLRNDAVSANNGS-AKVELRARLTEMTFNE 1146
            RRITTIDVLSNHRLNSF G+R DET+R V+KLRND V   N S   VELR RLTEMTFN 
Sbjct: 128  RRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNA 187

Query: 1145 MMMMISGKRYYGNDGDVTDVEEARQFREIITEILSLLGANNKGXXXXXXXXXXXXXLVKR 966
            MM MISGKRYYG+DGDVTDV+EA+QFR+II+EILSLLGANNKG             L KR
Sbjct: 188  MMRMISGKRYYGDDGDVTDVKEAKQFRDIISEILSLLGANNKGDFLPLLRLFDLDHLEKR 247

Query: 965  LKRIGKRSDAFLQGLIEQQRGGKHGGGDRDTMIDHLLKLKESQPEYYSDQTIKGLILAML 786
             KRI KR+DAFLQGLIEQ +   H   D DTMIDHLLKL+E+QPEYYSD  IKGLI AML
Sbjct: 248  CKRISKRADAFLQGLIEQHQNANHSDNDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAML 307

Query: 785  LAGTDTSAVTIEWAMSELLNNQEVLKKAKDEIDTQVGKERLVEEQDLPKLPYLQNIISET 606
            LAGTDTSAVTIEW M+ELLNN EVLKKAK EI+T +GKERLVEEQDL +LPYLQN+ISE 
Sbjct: 308  LAGTDTSAVTIEWVMAELLNNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEA 367

Query: 605  XXXXXXXXXXXXXXPSEDCTIGGFNVPRDTIILTNVWAIHRDPNLWSDADSFKPERFEKE 426
                           SEDCTIGGFNVP+DTIILTN+WAIHRDP LW+D  SFKPERFEKE
Sbjct: 368  LRLHPPAPLLLPHSASEDCTIGGFNVPKDTIILTNIWAIHRDPELWTDPSSFKPERFEKE 427

Query: 425  GEVNKLIAFGLGRRACPGLNLAQRTVGFTLGLLIQCFEWERESEEKLDMMEDKGITMPKR 246
            GEVNKL++FGLGRRACPGL+LAQRTVG+T+GLLIQ FEW+ ESEEKLD+ E KGITMP +
Sbjct: 428  GEVNKLLSFGLGRRACPGLSLAQRTVGYTVGLLIQGFEWKTESEEKLDLAEGKGITMPMK 487

Query: 245  IPLEAMCKPRPIVNDIMK 192
             PL AMCKP PIVNDIMK
Sbjct: 488  FPLRAMCKPLPIVNDIMK 505


>gb|AAQ20041.1| isoflavone 3'-hydroxylase, partial [Medicago truncatula]
          Length = 501

 Score =  618 bits (1593), Expect = e-174
 Identities = 314/438 (71%), Positives = 346/438 (78%), Gaps = 1/438 (0%)
 Frame = -1

Query: 1502 SLWFGXXXXXXXXXXXXVQECFNRNDVVLANRPKFLTGKYVFYNYTTLGSAPYGDHWRNL 1323
            SLWFG              ECF +ND++LANRP+FLTGKY+FYNYTTLGSA YGDHWRNL
Sbjct: 66   SLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNL 125

Query: 1322 RRITTIDVLSNHRLNSFVGIRSDETMRFVQKLRNDAVSANNGSAKVELRARLTEMTFNEM 1143
            RRITTIDVLSN+RLNSF+G+R DET R +QKL  D VS   G  KVELR RLTEMTFN M
Sbjct: 126  RRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAM 185

Query: 1142 MMMISGKRYYGNDGDVTDVEEARQFREIITEILSLLGANNKGXXXXXXXXXXXXXLVKRL 963
            M MISGKRYYG+DGDV+DVEEA+QFREII+E++SLLGANNKG             L KR 
Sbjct: 186  MRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRC 245

Query: 962  KRIGKRSDAFLQGLIEQQRGGK-HGGGDRDTMIDHLLKLKESQPEYYSDQTIKGLILAML 786
            KRI KRS+AFL+GLIE+ R G  H  G   TMIDHLLKL ESQPEYYSD  IKGLI  ML
Sbjct: 246  KRIAKRSNAFLEGLIEEHRRGNIHSDGG--TMIDHLLKLSESQPEYYSDHLIKGLIQGML 303

Query: 785  LAGTDTSAVTIEWAMSELLNNQEVLKKAKDEIDTQVGKERLVEEQDLPKLPYLQNIISET 606
            LAGTDTSAVTIEW MSELLN+ EVLKKAK+E+DTQ+GK +LV+EQDL KLPYLQNIISET
Sbjct: 304  LAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISET 363

Query: 605  XXXXXXXXXXXXXXPSEDCTIGGFNVPRDTIILTNVWAIHRDPNLWSDADSFKPERFEKE 426
                           SEDCTIG FNVP+DTIILTNVW IHRDP  W+DA SFKPERFEKE
Sbjct: 364  LRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFKPERFEKE 423

Query: 425  GEVNKLIAFGLGRRACPGLNLAQRTVGFTLGLLIQCFEWERESEEKLDMMEDKGITMPKR 246
             EVNK++AFGLGRRACPGL+LAQRTVGFT+GLLIQCFEWERESEEKLDMME KGITMP +
Sbjct: 424  EEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMMEGKGITMPMK 483

Query: 245  IPLEAMCKPRPIVNDIMK 192
            IPL AMCK  PI ND+ K
Sbjct: 484  IPLRAMCKALPIANDVTK 501


>gb|AAO31712.1| cytochrome P450 [Pisum sativum]
          Length = 502

 Score =  553 bits (1424), Expect = e-155
 Identities = 288/439 (65%), Positives = 329/439 (74%), Gaps = 2/439 (0%)
 Frame = -1

Query: 1502 SLWFGXXXXXXXXXXXXVQECFNRNDVVLANRPKFLTGKYVFYNYTTLGSAPYGDHWRNL 1323
            SLWFG            V+ECF +NDVVLANRP+FLTGKY+FY+YTTLGS  YGDHWRNL
Sbjct: 68   SLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNL 127

Query: 1322 RRITTIDVLSNHRLNSFVGIRSDETMRFVQKLRNDAVSANNGSAKVELRARLTEMTFNEM 1143
            RRITTIDVLSN+RL SF+G+R+DE  R +Q +     S +    KVELR+ LTEMTFN M
Sbjct: 128  RRITTIDVLSNNRLTSFLGVRTDEANRLIQNIIKHGASRD--FIKVELRSILTEMTFNGM 185

Query: 1142 MMMISGKRYYGNDGDVTDVEEARQFREIITEILSLLGANNKGXXXXXXXXXXXXXLVKRL 963
            M MISGKRYYG+DGDVT+VEEA+QFREII+EILSLLGANNKG             L KR 
Sbjct: 186  MRMISGKRYYGDDGDVTEVEEAKQFREIISEILSLLGANNKGDFLPLLRWFDLDDLEKRC 245

Query: 962  KRIGKRSDAFLQGLIEQQRGGKHGGGDRDTMIDHLLKLKESQPEYYSDQTIKGLILAMLL 783
            K+I  R+DAFL+GLI++ R   HG GD  TMIDHLLKL E Q        IKGLI AMLL
Sbjct: 246  KKIANRADAFLEGLIQEHRSANHGNGD--TMIDHLLKLSEVQLNIIPSHIIKGLIQAMLL 303

Query: 782  AGTDTSAVTIEWAMSELLNNQEVLKKAKDEIDTQVGKERLVEEQDLPKLPYLQNIISETX 603
            AGTDTSA+ IEW MSELLN+ EVL KAK+EI+TQVGK ++++EQDLPKLPYLQNIISE  
Sbjct: 304  AGTDTSALAIEWVMSELLNHPEVLNKAKEEIETQVGKNKIIDEQDLPKLPYLQNIISEAL 363

Query: 602  XXXXXXXXXXXXXPSEDCTIGGFNVPRDTIILTNVWAIHRDPNLWSDADSFKPERFEKEG 423
                          SEDCTIGGFNVP+DTIILTNVWAIHRDP  WSDA SFKPERFEKEG
Sbjct: 364  KLHPPAPLLLPHYSSEDCTIGGFNVPKDTIILTNVWAIHRDPTRWSDALSFKPERFEKEG 423

Query: 422  EVNKLIAFGLGRRACPGLNLAQRTVGFTLGLL-IQCFEWERESEEKLDMMEDKGITMPKR 246
            EVNKL+AFG+GRRACPGL+LAQRTVG   G    QCFEW+RESEEKLDMME K +   KR
Sbjct: 424  EVNKLMAFGIGRRACPGLSLAQRTVGLNCGACWFQCFEWKRESEEKLDMMEGKVLPCLKR 483

Query: 245  IPLEAMCKPRPIV-NDIMK 192
             P +A+CK   ++ ND+MK
Sbjct: 484  SPFKALCKGTTLLPNDLMK 502


>ref|XP_003532671.1| PREDICTED: isoflavone 2'-hydroxylase-like [Glycine max]
          Length = 500

 Score =  547 bits (1410), Expect = e-153
 Identities = 273/437 (62%), Positives = 326/437 (74%)
 Frame = -1

Query: 1502 SLWFGXXXXXXXXXXXXVQECFNRNDVVLANRPKFLTGKYVFYNYTTLGSAPYGDHWRNL 1323
            SLWFG            +QECF ++D+VLANRP+FLTGKY+FYNY+++GS+PYGDHWRNL
Sbjct: 63   SLWFGSRFVVVISSPTLLQECFTKHDIVLANRPRFLTGKYLFYNYSSMGSSPYGDHWRNL 122

Query: 1322 RRITTIDVLSNHRLNSFVGIRSDETMRFVQKLRNDAVSANNGSAKVELRARLTEMTFNEM 1143
            RRI TIDVLS  RLNSF  IR +ETMR +QKL  +     NG A V LR RLTEMTFN M
Sbjct: 123  RRIITIDVLSTSRLNSFFEIRREETMRVIQKLARETC---NGFALVHLRPRLTEMTFNNM 179

Query: 1142 MMMISGKRYYGNDGDVTDVEEARQFREIITEILSLLGANNKGXXXXXXXXXXXXXLVKRL 963
            M MISGKRYYG+D +  D EEA+QFR+I+TE++SLLGANNKG             L KRL
Sbjct: 180  MRMISGKRYYGDDIEAADAEEAKQFRDIMTEVMSLLGANNKGDFLPFLRWFDFDGLEKRL 239

Query: 962  KRIGKRSDAFLQGLIEQQRGGKHGGGDRDTMIDHLLKLKESQPEYYSDQTIKGLILAMLL 783
            K I  R+D+FLQGL+E+ R GKH     +TMI+HLL ++ESQP YYSD  IKGLI  MLL
Sbjct: 240  KVISTRADSFLQGLLEEHRSGKHKA---NTMIEHLLTMQESQPHYYSDHIIKGLIQGMLL 296

Query: 782  AGTDTSAVTIEWAMSELLNNQEVLKKAKDEIDTQVGKERLVEEQDLPKLPYLQNIISETX 603
            AGTDT+AV IEWA+S LLN+ E+LKKAKDEID  VG++RLV+E D+PKLPYLQNII ET 
Sbjct: 297  AGTDTTAVAIEWAVSSLLNHPEILKKAKDEIDNMVGQDRLVDESDIPKLPYLQNIIYETL 356

Query: 602  XXXXXXXXXXXXXPSEDCTIGGFNVPRDTIILTNVWAIHRDPNLWSDADSFKPERFEKEG 423
                          SE+CTIGGF +PRDTI+L N WAI RDP  WSDA  FKPERFE+EG
Sbjct: 357  RLFAPAPLLLPHYSSEECTIGGFTIPRDTIVLINAWAIQRDPEHWSDATCFKPERFEQEG 416

Query: 422  EVNKLIAFGLGRRACPGLNLAQRTVGFTLGLLIQCFEWERESEEKLDMMEDKGITMPKRI 243
            E NKLI FGLGRRACPG+ LA R++G TLGLLIQCFEW+R ++E++DM E+KG+ +PK I
Sbjct: 417  EANKLIPFGLGRRACPGIGLAHRSMGLTLGLLIQCFEWKRPTDEEIDMRENKGLALPKLI 476

Query: 242  PLEAMCKPRPIVNDIMK 192
            PLEAM K RPI   +M+
Sbjct: 477  PLEAMFKTRPISEKVMQ 493


>ref|XP_003548043.1| PREDICTED: isoflavone 2'-hydroxylase-like [Glycine max]
          Length = 498

 Score =  530 bits (1364), Expect = e-148
 Identities = 263/427 (61%), Positives = 318/427 (74%)
 Frame = -1

Query: 1502 SLWFGXXXXXXXXXXXXVQECFNRNDVVLANRPKFLTGKYVFYNYTTLGSAPYGDHWRNL 1323
            SLWFG            VQECF +ND+VLANRP FLTGKY+ YN TT+  +PYGDHWRNL
Sbjct: 65   SLWFGSRFVVVVSSPLAVQECFTKNDIVLANRPHFLTGKYIGYNNTTVAVSPYGDHWRNL 124

Query: 1322 RRITTIDVLSNHRLNSFVGIRSDETMRFVQKLRNDAVSANNGSAKVELRARLTEMTFNEM 1143
            RRI  ++VLS HR+NSF+  R DE MR VQKL  D+    NG  KVEL++R +EMTFN +
Sbjct: 125  RRIMALEVLSTHRINSFLENRRDEIMRLVQKLARDS---RNGFTKVELKSRFSEMTFNTI 181

Query: 1142 MMMISGKRYYGNDGDVTDVEEARQFREIITEILSLLGANNKGXXXXXXXXXXXXXLVKRL 963
            M M+SGKRYYG D DV+DV+EARQFREII E+++L GANN G             L KRL
Sbjct: 182  MRMVSGKRYYGEDCDVSDVQEARQFREIIKELVTLGGANNPGDFLALLRWFDFDGLEKRL 241

Query: 962  KRIGKRSDAFLQGLIEQQRGGKHGGGDRDTMIDHLLKLKESQPEYYSDQTIKGLILAMLL 783
            KRI KR+DAFLQGLI+Q R GKH     +TMIDHLL  ++SQPEYY+DQ IKGL L MLL
Sbjct: 242  KRISKRTDAFLQGLIDQHRNGKHRA---NTMIDHLLAQQQSQPEYYTDQIIKGLALVMLL 298

Query: 782  AGTDTSAVTIEWAMSELLNNQEVLKKAKDEIDTQVGKERLVEEQDLPKLPYLQNIISETX 603
            AGTDTSAVT+EWAMS LLN+ E+LKKAK+E+DT +G++RLV+E D+PKLPYLQ+I+ ET 
Sbjct: 299  AGTDTSAVTLEWAMSNLLNHPEILKKAKNELDTHIGQDRLVDEPDIPKLPYLQSIVYETL 358

Query: 602  XXXXXXXXXXXXXPSEDCTIGGFNVPRDTIILTNVWAIHRDPNLWSDADSFKPERFEKEG 423
                          SEDCTIG +N+P++TI+L N WAIHRDP LWSD   FKPERFE E 
Sbjct: 359  RLHPAAPMLVPHLSSEDCTIGEYNIPQNTILLVNAWAIHRDPKLWSDPTHFKPERFENES 418

Query: 422  EVNKLIAFGLGRRACPGLNLAQRTVGFTLGLLIQCFEWERESEEKLDMMEDKGITMPKRI 243
            E NKL+ FGLGRRACPG NLAQRT+  TL LLIQCFEW+R +++++DM E KG+T+ K+ 
Sbjct: 419  EANKLLPFGLGRRACPGANLAQRTLSLTLALLIQCFEWKRTTKKEIDMTEGKGLTVSKKY 478

Query: 242  PLEAMCK 222
            PLEAMC+
Sbjct: 479  PLEAMCQ 485