BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Glycyrrhiza24_contig00002327
         (1566 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|ACJ85868.1| unknown [Medicago truncatula] gi|388518991|gb|AFK...   696   0.0  
ref|XP_003548043.1| PREDICTED: isoflavone 2'-hydroxylase-like [G...   607   e-171
ref|XP_003611658.1| Isoflavone 2'-hydroxylase [Medicago truncatu...   573   e-161
gb|AAQ20042.1| CYP81E8 [Medicago truncatula]                          572   e-160
ref|XP_003532671.1| PREDICTED: isoflavone 2'-hydroxylase-like [G...   527   e-147

>gb|ACJ85868.1| unknown [Medicago truncatula] gi|388518991|gb|AFK47557.1| unknown
            [Medicago truncatula]
          Length = 509

 Score =  696 bits (1797), Expect = 0.0
 Identities = 345/420 (82%), Positives = 375/420 (89%), Gaps = 7/420 (1%)
 Frame = -2

Query: 1565 KNDIVLANRPPLLAAKHIGYNFTAVTVAPYGDHWRNVRRIISLEVLSTHRLNSFIGIRRD 1386
            KND +LANRPPLLA KHIGYN+TAVTVAPYGDHWRNVRRIISL+VLSTHRLNSF+ IR++
Sbjct: 90   KNDTILANRPPLLAGKHIGYNYTAVTVAPYGDHWRNVRRIISLDVLSTHRLNSFLRIRKN 149

Query: 1385 EITRLVKNLARGSH----EGFAEVELKTKLSEMTFNSIMRMISGKRYYGEDCD--DVEEA 1224
            EI +L++ LARGS     +GF  VELKTKLSEMTFN+IMRMISGKRYYGEDCD  D EEA
Sbjct: 150  EIMKLMQALARGSSSSSSDGFVRVELKTKLSEMTFNTIMRMISGKRYYGEDCDVSDEEEA 209

Query: 1223 RQFRELAKELISFGGSSNPAEFVGVLRWFDFGNLEKSLKRIGSRCDALLQGLIEEHRSGK 1044
            + FRE+ KE++S GGSSNP EFVG+LRWFDFG  EK LKRI  R D  LQGLI+EHR  K
Sbjct: 210  KGFREMIKEMVSLGGSSNPGEFVGILRWFDFGGYEKKLKRIARRFDGFLQGLIDEHRRKK 269

Query: 1043 QSGDTMIDHLLTLQQ-SQPEYYTDQIIKGIVLVMILGGTETSATTLEWAMSALLNHPEVL 867
            + G+ MIDHLL LQ+ SQPEYYTDQIIKGIVLVMILGGTETSATTLEWAMSALLNHPEVL
Sbjct: 270  EKGNNMIDHLLNLQELSQPEYYTDQIIKGIVLVMILGGTETSATTLEWAMSALLNHPEVL 329

Query: 866  KKARDEIDAQIGQDRLVEESDISRLPYLQNIIHETFRLHPAFALLAPHFSSKDCTIGGYH 687
            KKARDEID  IGQD LVEESDIS+LPYLQNIIHETFRLHPAFALLAPHFSS+DCTIGGY 
Sbjct: 330  KKARDEIDTNIGQDHLVEESDISKLPYLQNIIHETFRLHPAFALLAPHFSSQDCTIGGYI 389

Query: 686  VPQGTILLVNAWAIHRDPQLWSDPTQFKPERFEKEGEADKLIPFGLGRRACPGANLGQRT 507
            VP+ TILLVNAWAIHRD QLWSDPTQFKPERF+KEGEADKLIPFGLGRRACPGANLGQRT
Sbjct: 390  VPKNTILLVNAWAIHRDSQLWSDPTQFKPERFDKEGEADKLIPFGLGRRACPGANLGQRT 449

Query: 506  VSYTLGLLIQCFEWKRMSEEEIDMTEGKGATTPKLIPLKAMCQARSNITNKVFLKAGEEM 327
            VS TL LLIQCF+WKR+SEEEIDMTEGKGATTPKLIPL+AMC+ARSN+ NKV+LK  E M
Sbjct: 450  VSLTLALLIQCFDWKRISEEEIDMTEGKGATTPKLIPLEAMCKARSNVINKVYLKVDENM 509


>ref|XP_003548043.1| PREDICTED: isoflavone 2'-hydroxylase-like [Glycine max]
          Length = 498

 Score =  607 bits (1565), Expect = e-171
 Identities = 289/406 (71%), Positives = 341/406 (83%), Gaps = 2/406 (0%)
 Frame = -2

Query: 1565 KNDIVLANRPPLLAAKHIGYNFTAVTVAPYGDHWRNVRRIISLEVLSTHRLNSFIGIRRD 1386
            KNDIVLANRP  L  K+IGYN T V V+PYGDHWRN+RRI++LEVLSTHR+NSF+  RRD
Sbjct: 88   KNDIVLANRPHFLTGKYIGYNNTTVAVSPYGDHWRNLRRIMALEVLSTHRINSFLENRRD 147

Query: 1385 EITRLVKNLARGSHEGFAEVELKTKLSEMTFNSIMRMISGKRYYGEDCD--DVEEARQFR 1212
            EI RLV+ LAR S  GF +VELK++ SEMTFN+IMRM+SGKRYYGEDCD  DV+EARQFR
Sbjct: 148  EIMRLVQKLARDSRNGFTKVELKSRFSEMTFNTIMRMVSGKRYYGEDCDVSDVQEARQFR 207

Query: 1211 ELAKELISFGGSSNPAEFVGVLRWFDFGNLEKSLKRIGSRCDALLQGLIEEHRSGKQSGD 1032
            E+ KEL++ GG++NP +F+ +LRWFDF  LEK LKRI  R DA LQGLI++HR+GK   +
Sbjct: 208  EIIKELVTLGGANNPGDFLALLRWFDFDGLEKRLKRISKRTDAFLQGLIDQHRNGKHRAN 267

Query: 1031 TMIDHLLTLQQSQPEYYTDQIIKGIVLVMILGGTETSATTLEWAMSALLNHPEVLKKARD 852
            TMIDHLL  QQSQPEYYTDQIIKG+ LVM+L GT+TSA TLEWAMS LLNHPE+LKKA++
Sbjct: 268  TMIDHLLAQQQSQPEYYTDQIIKGLALVMLLAGTDTSAVTLEWAMSNLLNHPEILKKAKN 327

Query: 851  EIDAQIGQDRLVEESDISRLPYLQNIIHETFRLHPAFALLAPHFSSKDCTIGGYHVPQGT 672
            E+D  IGQDRLV+E DI +LPYLQ+I++ET RLHPA  +L PH SS+DCTIG Y++PQ T
Sbjct: 328  ELDTHIGQDRLVDEPDIPKLPYLQSIVYETLRLHPAAPMLVPHLSSEDCTIGEYNIPQNT 387

Query: 671  ILLVNAWAIHRDPQLWSDPTQFKPERFEKEGEADKLIPFGLGRRACPGANLGQRTVSYTL 492
            ILLVNAWAIHRDP+LWSDPT FKPERFE E EA+KL+PFGLGRRACPGANL QRT+S TL
Sbjct: 388  ILLVNAWAIHRDPKLWSDPTHFKPERFENESEANKLLPFGLGRRACPGANLAQRTLSLTL 447

Query: 491  GLLIQCFEWKRMSEEEIDMTEGKGATTPKLIPLKAMCQARSNITNK 354
             LLIQCFEWKR +++EIDMTEGKG T  K  PL+AMCQ   ++T K
Sbjct: 448  ALLIQCFEWKRTTKKEIDMTEGKGLTVSKKYPLEAMCQVCQSLTVK 493


>ref|XP_003611658.1| Isoflavone 2'-hydroxylase [Medicago truncatula]
            gi|355512993|gb|AES94616.1| Isoflavone 2'-hydroxylase
            [Medicago truncatula]
          Length = 499

 Score =  573 bits (1478), Expect = e-161
 Identities = 272/400 (68%), Positives = 327/400 (81%), Gaps = 2/400 (0%)
 Frame = -2

Query: 1565 KNDIVLANRPPLLAAKHIGYNFTAVTVAPYGDHWRNVRRIISLEVLSTHRLNSFIGIRRD 1386
            KNDIVLANRP  L  K+IGYN T V  +PYGDHWRN+RRI+S+E+LS+HRLNSF+ IRRD
Sbjct: 91   KNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNLRRILSIEILSSHRLNSFLEIRRD 150

Query: 1385 EITRLVKNLARGSHEGFAEVELKTKLSEMTFNSIMRMISGKRYYGEDCD--DVEEARQFR 1212
            EI RL++ LA+ S+ GF EVEL+   SEMTFN+IMRM+SGKRYYG DCD  DVEEAR FR
Sbjct: 151  EIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTIMRMVSGKRYYGNDCDVSDVEEARLFR 210

Query: 1211 ELAKELISFGGSSNPAEFVGVLRWFDFGNLEKSLKRIGSRCDALLQGLIEEHRSGKQSGD 1032
             + KE++S GG++N  +F+G LRWFDF  LEK LK+I  R DA LQGLI+EHR GK++ +
Sbjct: 211  GIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLKKISKRTDAFLQGLIDEHRFGKRNSN 270

Query: 1031 TMIDHLLTLQQSQPEYYTDQIIKGIVLVMILGGTETSATTLEWAMSALLNHPEVLKKARD 852
            TMIDHLLT QQSQPEYYTDQIIKG+++VM+L GT+TS+ T+EWAMS LLNHPE++KKA++
Sbjct: 271  TMIDHLLTQQQSQPEYYTDQIIKGLMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKN 330

Query: 851  EIDAQIGQDRLVEESDISRLPYLQNIIHETFRLHPAFALLAPHFSSKDCTIGGYHVPQGT 672
            E+D  IG DR V+E DIS+LPYLQ+I++ET RLH A  LL PH SS+D ++GGY++PQ T
Sbjct: 331  ELDTHIGHDRQVDEHDISKLPYLQSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNT 390

Query: 671  ILLVNAWAIHRDPQLWSDPTQFKPERFEKEGEADKLIPFGLGRRACPGANLGQRTVSYTL 492
            IL+VNAW IHRDP LWSDPT FKPERFEKEGE +KL+ FGLGRRACPG NL QRTV  TL
Sbjct: 391  ILMVNAWVIHRDPNLWSDPTCFKPERFEKEGEVNKLLSFGLGRRACPGENLAQRTVGLTL 450

Query: 491  GLLIQCFEWKRMSEEEIDMTEGKGATTPKLIPLKAMCQAR 372
            GLLIQCFEWKR+ EE+IDM E KG T  K   L AMC+ R
Sbjct: 451  GLLIQCFEWKRIGEEKIDMVEAKGITVGKKTSLNAMCKVR 490


>gb|AAQ20042.1| CYP81E8 [Medicago truncatula]
          Length = 499

 Score =  572 bits (1473), Expect = e-160
 Identities = 274/409 (66%), Positives = 330/409 (80%), Gaps = 3/409 (0%)
 Frame = -2

Query: 1565 KNDIVLANRPPLLAAKHIGYNFTAVTVAPYGDHWRNVRRIISLEVLSTHRLNSFIGIRRD 1386
            KNDIVLANRP  L  K+IGYN T V  +PYGDHWRN+RRI+S+E+LS+HRLNSF+ IRRD
Sbjct: 91   KNDIVLANRPHFLTGKYIGYNNTTVAQSPYGDHWRNLRRILSIEILSSHRLNSFLEIRRD 150

Query: 1385 EITRLVKNLARGSHEGFAEVELKTKLSEMTFNSIMRMISGKRYYGEDCD--DVEEARQFR 1212
            EI RL++ LA+ S+ GF EVEL+   SEMTFN+IMRM+SGKRYYG DCD  DVEEAR FR
Sbjct: 151  EIMRLIQKLAQKSYNGFTEVELRPMFSEMTFNTIMRMVSGKRYYGNDCDVSDVEEARLFR 210

Query: 1211 ELAKELISFGGSSNPAEFVGVLRWFDFGNLEKSLKRIGSRCDALLQGLIEEHRSGKQSGD 1032
             + KE++S GG++N  +F+G LRWFDF  LEK LK+I  R DA LQGLI+EHR GK++ +
Sbjct: 211  GIIKEVVSLGGANNVGDFLGFLRWFDFDGLEKRLKKISKRTDAFLQGLIDEHRFGKRNSN 270

Query: 1031 TMIDHLLTLQQSQPEYYTDQIIKGIVLVMILGGTETSATTLEWAMSALLNHPEVLKKARD 852
            TMIDHLLT QQSQPEYYTDQIIKG+++VM+L GT+TS+ T+EWAMS LLNHPE++KKA++
Sbjct: 271  TMIDHLLTQQQSQPEYYTDQIIKGLMVVMLLAGTDTSSVTIEWAMSNLLNHPEIMKKAKN 330

Query: 851  EIDAQIGQDRLVEESDISRLPYLQNIIHETFRLHPAFALLAPHFSSKDCTIGGYHVPQGT 672
            E+D  IG DR V+E DIS+LPYLQ+I++ET RLH A  LL PH SS+D ++GGY++PQ T
Sbjct: 331  ELDTHIGHDRQVDEHDISKLPYLQSIVYETLRLHAAAPLLVPHLSSEDFSLGGYNIPQNT 390

Query: 671  ILLVNAWAIHRDPQLWSDPTQFKPERFEKEGEADKLIPFGLGRRACPGANLGQRTVSYTL 492
            IL+VNAW IHRDP LWSDPT FKPERFEKEGE +KL+ FGLGRRACPG NL QRT   TL
Sbjct: 391  ILMVNAWVIHRDPNLWSDPTCFKPERFEKEGEVNKLLSFGLGRRACPGENLSQRTEGLTL 450

Query: 491  GLLIQCFEWKRMSEEEIDMTEGKGATTPKLIPLKAMCQARSNI-TNKVF 348
            GLLIQCFEWKR+ EE+IDM E KG T  K   L AMC+ R  +  N VF
Sbjct: 451  GLLIQCFEWKRIGEEKIDMVEAKGITAGKKTSLNAMCKVRHPLKINDVF 499


>ref|XP_003532671.1| PREDICTED: isoflavone 2'-hydroxylase-like [Glycine max]
          Length = 500

 Score =  527 bits (1358), Expect = e-147
 Identities = 253/407 (62%), Positives = 326/407 (80%), Gaps = 2/407 (0%)
 Frame = -2

Query: 1565 KNDIVLANRPPLLAAKHIGYNFTAVTVAPYGDHWRNVRRIISLEVLSTHRLNSFIGIRRD 1386
            K+DIVLANRP  L  K++ YN++++  +PYGDHWRN+RRII+++VLST RLNSF  IRR+
Sbjct: 86   KHDIVLANRPRFLTGKYLFYNYSSMGSSPYGDHWRNLRRIITIDVLSTSRLNSFFEIRRE 145

Query: 1385 EITRLVKNLARGSHEGFAEVELKTKLSEMTFNSIMRMISGKRYYGEDCD--DVEEARQFR 1212
            E  R+++ LAR +  GFA V L+ +L+EMTFN++MRMISGKRYYG+D +  D EEA+QFR
Sbjct: 146  ETMRVIQKLARETCNGFALVHLRPRLTEMTFNNMMRMISGKRYYGDDIEAADAEEAKQFR 205

Query: 1211 ELAKELISFGGSSNPAEFVGVLRWFDFGNLEKSLKRIGSRCDALLQGLIEEHRSGKQSGD 1032
            ++  E++S  G++N  +F+  LRWFDF  LEK LK I +R D+ LQGL+EEHRSGK   +
Sbjct: 206  DIMTEVMSLLGANNKGDFLPFLRWFDFDGLEKRLKVISTRADSFLQGLLEEHRSGKHKAN 265

Query: 1031 TMIDHLLTLQQSQPEYYTDQIIKGIVLVMILGGTETSATTLEWAMSALLNHPEVLKKARD 852
            TMI+HLLT+Q+SQP YY+D IIKG++  M+L GT+T+A  +EWA+S+LLNHPE+LKKA+D
Sbjct: 266  TMIEHLLTMQESQPHYYSDHIIKGLIQGMLLAGTDTTAVAIEWAVSSLLNHPEILKKAKD 325

Query: 851  EIDAQIGQDRLVEESDISRLPYLQNIIHETFRLHPAFALLAPHFSSKDCTIGGYHVPQGT 672
            EID  +GQDRLV+ESDI +LPYLQNII+ET RL     LL PH+SS++CTIGG+ +P+ T
Sbjct: 326  EIDNMVGQDRLVDESDIPKLPYLQNIIYETLRLFAPAPLLLPHYSSEECTIGGFTIPRDT 385

Query: 671  ILLVNAWAIHRDPQLWSDPTQFKPERFEKEGEADKLIPFGLGRRACPGANLGQRTVSYTL 492
            I+L+NAWAI RDP+ WSD T FKPERFE+EGEA+KLIPFGLGRRACPG  L  R++  TL
Sbjct: 386  IVLINAWAIQRDPEHWSDATCFKPERFEQEGEANKLIPFGLGRRACPGIGLAHRSMGLTL 445

Query: 491  GLLIQCFEWKRMSEEEIDMTEGKGATTPKLIPLKAMCQARSNITNKV 351
            GLLIQCFEWKR ++EEIDM E KG   PKLIPL+AM + R  I+ KV
Sbjct: 446  GLLIQCFEWKRPTDEEIDMRENKGLALPKLIPLEAMFKTRP-ISEKV 491