BLASTX nr result
ID: Glycyrrhiza23_contig00013954
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Glycyrrhiza23_contig00013954
(1378 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_003549135.1| PREDICTED: 13-hydroxylupanine O-tigloyltrans... 589 e-166
gb|ACJ85557.1| unknown [Medicago truncatula] 507 e-141
gb|AFK36385.1| unknown [Medicago truncatula] 505 e-140
sp|Q5H873.1|HLTT_LUPAL RecName: Full=13-hydroxylupanine O-tigloy... 502 e-139
gb|ABN48480.1| quinolizidine alkaloid O-tigloyltransferase [Lupi... 501 e-139
>ref|XP_003549135.1| PREDICTED: 13-hydroxylupanine O-tigloyltransferase-like [Glycine max]
Length = 455
Score = 589 bits (1519), Expect = e-166
Identities = 292/423 (69%), Positives = 344/423 (81%), Gaps = 3/423 (0%)
Frame = -3
Query: 1376 KLLSDIDDQNDMRFHMPIVHFYRYQPLMAGKDPVQLIREALAKTLVFYYPFAGRLKEGPK 1197
KLLSDIDDQN +R+ +P+V F+ YQP M GKDPV++IREAL+KTLVFYYPFAGRL+EGP
Sbjct: 31 KLLSDIDDQNGLRYQLPLVLFFPYQPSMEGKDPVEVIREALSKTLVFYYPFAGRLREGPD 90
Query: 1196 GKLMVDCTGEGVMFIEADADVTLEQFGNDLLPPFPCFDELLYNVPGSEGLTGCPLLLIQV 1017
GKLMVDC GEGVMFIEADADVT+EQFGN+ +PPFPCFDELLYNVPGS+G+ PLLLIQV
Sbjct: 91 GKLMVDCNGEGVMFIEADADVTIEQFGNNFMPPFPCFDELLYNVPGSDGMIDTPLLLIQV 150
Query: 1016 TRLRCGGFIFAYRWNHTMSDASGISQFLNALAEIACGAPKPSILPVWCRELLCARDPPRV 837
TRL+CGGFIFA R NHTM D SGI QFL AL+EIA GAPKPSILP W RE+LCAR+PPR+
Sbjct: 151 TRLKCGGFIFALRMNHTMCDGSGICQFLKALSEIAHGAPKPSILPGWHREILCAREPPRI 210
Query: 836 TCAHDKEYQQELPVRNTKSILKLHHRSFFFGPTEIATIRALLPHHLAAQSTSFEVLTACI 657
TC H +EYQQ P +++SI H RSFFFGP EIA++RALLPHHLA +STSFEV+TAC+
Sbjct: 211 TCIH-QEYQQLPP--DSRSIFIPHQRSFFFGPKEIASLRALLPHHLATKSTSFEVITACL 267
Query: 656 WQCRTKALHWQTRDEEIRFMGVVNARYGNCRFNPPLPIGFYGNASVVCLPAITSDTTVGK 477
W+CRT +L WQ ++E+R + +VNAR+GNCRFNPPLP GFYGNA V PA TTVGK
Sbjct: 268 WRCRTASLKWQNPNQEVRLLCIVNARFGNCRFNPPLPDGFYGNAFV--FPAAV--TTVGK 323
Query: 476 LCSEPLEYALKLVKEAKGKATEEYVHSVVDFMAIKGRPWFKSFGCFVVSDLSKAALSDVD 297
L L YA++LVK+AK +A EEYVHSV D MAIKGRP F G F+VSDL+K+ L DV+
Sbjct: 324 LLGRSLGYAVELVKKAKDEADEEYVHSVADLMAIKGRPCFTKLGSFMVSDLTKSGLIDVN 383
Query: 296 FGWGKAVYAGPPNILVTE---ASFFVPFTNSKGERGRVVIICLPGDAMERFEKELNDMLK 126
GWGKA+Y+G + + SF+VP+TNSKGERGRV+ ICLP DAMERFEKEL+D L
Sbjct: 384 LGWGKALYSGVAKGGLGDIPGVSFYVPYTNSKGERGRVIPICLPEDAMERFEKELHDTLM 443
Query: 125 IKN 117
IKN
Sbjct: 444 IKN 446
>gb|ACJ85557.1| unknown [Medicago truncatula]
Length = 459
Score = 507 bits (1306), Expect = e-141
Identities = 259/425 (60%), Positives = 319/425 (75%), Gaps = 8/425 (1%)
Frame = -3
Query: 1376 KLLSDIDDQNDMRFHMPIVHFYRYQPLMAGKDPVQLIREALAKTLVFYYPFAGRLKEGPK 1197
KLLSDIDDQ +RF +P++ FY+Y P MAGKDPV +IR+A AKTLVFYYPFAGRL+EGP
Sbjct: 28 KLLSDIDDQEGLRFQIPVIQFYKYDPNMAGKDPVDIIRKASAKTLVFYYPFAGRLREGPG 87
Query: 1196 GKLMVDCTGEGVMFIEADADVTLEQFGNDLLPPFPCFDELLYNVPGSEGLTGCPLLLIQV 1017
KLMVDCTGEGV+FIEADADV+L++FG+ L PPFPC DELL++VPGS + CPLL+IQV
Sbjct: 88 RKLMVDCTGEGVLFIEADADVSLKEFGDALQPPFPCLDELLFDVPGSSEVLNCPLLVIQV 147
Query: 1016 TRLRCGGFIFAYRWNHTMSDASGISQFLNALAEIACGAPKPSILPVWCRELLCARDPPRV 837
TRL+CGGFIFA R NHTMSDA+G+ QF++AL EI+CG +PSILPVW RELL +RDPPRV
Sbjct: 148 TRLKCGGFIFALRLNHTMSDAAGLVQFMSALGEISCGMNEPSILPVWHRELLNSRDPPRV 207
Query: 836 TCAHDKEYQQELPVRNTKSIL----KLHHRSFFFGPTEIATIRALLPHHLAAQSTSFEVL 669
TC H +EY+Q V +TK + + HRSFFFGPTE+A IRALLP H QS +FE+L
Sbjct: 208 TCTH-REYEQ---VPDTKGTIIPLDDMAHRSFFFGPTEVAKIRALLPPHQQKQS-NFEIL 262
Query: 668 TACIWQCRTKALHWQTRDEEIRFMGVVNARYGNCRFNPPLPIGFYGNASVVCLPAITSDT 489
TAC+W+CRT AL T DEE+R + +VNAR +FNPPLP G+YGNA + T
Sbjct: 263 TACLWRCRTIALQPDT-DEEVRIICIVNAR---GKFNPPLPNGYYGNAFAFPVAV----T 314
Query: 488 TVGKLCSEPLEYALKLVKEAKGKATEEYVHSVVDFMAIKGRPWFKSFGCFVVSDLSKAAL 309
T GKL PL YAL+LVK+AK T+EY+HSV D M IKGRP F ++VSD+++A
Sbjct: 315 TAGKLMENPLGYALELVKKAKSDVTQEYMHSVADLMVIKGRPHFTVVRSYLVSDVTRAGF 374
Query: 308 SDVDFGWGKAVYAGPP----NILVTEASFFVPFTNSKGERGRVVIICLPGDAMERFEKEL 141
DV+FGWGKAVY GP + ASF++PF N+KGE G V+ +CLP AME+F KEL
Sbjct: 375 GDVEFGWGKAVYGGPAKGGVGAIPGVASFYIPFKNAKGEEGLVIPLCLPAQAMEKFVKEL 434
Query: 140 NDMLK 126
+ +LK
Sbjct: 435 DSVLK 439
>gb|AFK36385.1| unknown [Medicago truncatula]
Length = 459
Score = 505 bits (1300), Expect = e-140
Identities = 258/425 (60%), Positives = 318/425 (74%), Gaps = 8/425 (1%)
Frame = -3
Query: 1376 KLLSDIDDQNDMRFHMPIVHFYRYQPLMAGKDPVQLIREALAKTLVFYYPFAGRLKEGPK 1197
KLLSDIDDQ +RF +P++ FY+Y P MAGKDPV +IR+A AKTLVFYYPFAGRL+EGP
Sbjct: 28 KLLSDIDDQEGLRFQIPVIQFYKYDPNMAGKDPVDIIRKASAKTLVFYYPFAGRLREGPG 87
Query: 1196 GKLMVDCTGEGVMFIEADADVTLEQFGNDLLPPFPCFDELLYNVPGSEGLTGCPLLLIQV 1017
KLMVDCTGEGV+FIEADADV+L++FG+ L PPFPC DELL++VPGS + CPLL+IQV
Sbjct: 88 RKLMVDCTGEGVLFIEADADVSLKEFGDALQPPFPCLDELLFDVPGSSEVLNCPLLVIQV 147
Query: 1016 TRLRCGGFIFAYRWNHTMSDASGISQFLNALAEIACGAPKPSILPVWCRELLCARDPPRV 837
TRL+CGGFIFA R NHTMSDA+G+ QF++AL EI+CG +PSILPVW RELL +RDPPRV
Sbjct: 148 TRLKCGGFIFALRLNHTMSDAAGLVQFMSALGEISCGMNEPSILPVWHRELLNSRDPPRV 207
Query: 836 TCAHDKEYQQELPVRNTKSIL----KLHHRSFFFGPTEIATIRALLPHHLAAQSTSFEVL 669
TC H +EY+Q V +TK + + HRSFFFGPTE+A IRALLP H QS +FE+L
Sbjct: 208 TCTH-REYEQ---VPDTKGTIIPLDDMAHRSFFFGPTEVAKIRALLPPHQQKQS-NFEIL 262
Query: 668 TACIWQCRTKALHWQTRDEEIRFMGVVNARYGNCRFNPPLPIGFYGNASVVCLPAITSDT 489
TAC+W+CRT AL T DEE+R + +VNAR +FNPPLP G+YGNA + T
Sbjct: 263 TACLWRCRTIALQPDT-DEEVRIICIVNAR---GKFNPPLPNGYYGNAFAFPVAV----T 314
Query: 488 TVGKLCSEPLEYALKLVKEAKGKATEEYVHSVVDFMAIKGRPWFKSFGCFVVSDLSKAAL 309
T GKL PL YAL+LVK+AK T+EY+HSV D M IKGRP F ++V D+++A
Sbjct: 315 TAGKLMENPLGYALELVKKAKSDVTQEYMHSVADLMVIKGRPHFTVVRSYLVFDVTRAGF 374
Query: 308 SDVDFGWGKAVYAGPP----NILVTEASFFVPFTNSKGERGRVVIICLPGDAMERFEKEL 141
DV+FGWGKAVY GP + ASF++PF N+KGE G V+ +CLP AME+F KEL
Sbjct: 375 GDVEFGWGKAVYGGPAKGGVGAIPGVASFYIPFKNAKGEEGLVIPLCLPAQAMEKFVKEL 434
Query: 140 NDMLK 126
+ +LK
Sbjct: 435 DSVLK 439
>sp|Q5H873.1|HLTT_LUPAL RecName: Full=13-hydroxylupanine O-tigloyltransferase; AltName:
Full=(-)-13alpha-hydroxymultiflorine/(+)-13alpha-
hydroxylupanine O-tigloyltransferase; Short=HMT/HLTase;
AltName: Full=Quinolizidine alkaloid O-tigloyltransferase
gi|58530624|dbj|BAD89275.1|
(-)-13alpha-hydroxymultiflorine/(+)-13alpha-
hydroxylupanine O-tigloyltransferase [Lupinus albus]
Length = 453
Score = 502 bits (1292), Expect = e-139
Identities = 262/422 (62%), Positives = 311/422 (73%), Gaps = 2/422 (0%)
Frame = -3
Query: 1376 KLLSDIDDQNDMRFHMPIVHFYRYQPLMAGKDPVQLIREALAKTLVFYYPFAGRLKEGPK 1197
KLLSDIDDQ +R P+V YR P M GKDPV++IREAL+KTLVFYYPFAGRL+ GP
Sbjct: 31 KLLSDIDDQTSLRSLTPLVTIYRNNPSMEGKDPVEIIREALSKTLVFYYPFAGRLRNGPN 90
Query: 1196 GKLMVDCTGEGVMFIEADADVTLEQFGNDLLPPFPCFDELLYNVPGSEGLTGCPLLLIQV 1017
GKLMVDCTGEGV+FIEADADVTL+QFG DL PPFPCFD+LLY+VPGS+G+ PLLLIQV
Sbjct: 91 GKLMVDCTGEGVIFIEADADVTLDQFGIDLHPPFPCFDQLLYDVPGSDGILDSPLLLIQV 150
Query: 1016 TRLRCGGFIFAYRWNHTMSDASGISQFLNALAEIACGAPKPSILPVWCRELLCARDPPRV 837
TRL+CGGFIFA R NH M DA G+SQF+ LAEIA G PKP ILPVW RELLCAR+PP+V
Sbjct: 151 TRLKCGGFIFAVRLNHAMCDAIGMSQFMKGLAEIARGEPKPFILPVWHRELLCARNPPKV 210
Query: 836 TCAHDKEYQQELPVRNTKSILKLHHRSFFFGPTEIATIRALLPHHLAAQSTSFEVLTACI 657
T H+ EYQ+ P N + L H SFFFGP E+ IR LLP+H ++ST+ ++LTA +
Sbjct: 211 TFIHN-EYQKP-PHDNNNNNFILQHSSFFFGPNELDAIRRLLPYH-HSKSTTSDILTAFL 267
Query: 656 WQCRTKALHWQTRDEEIRFMGVVNARYGNCRFNPPLPIGFYGNASVVCLPAITSDTTVGK 477
W+CRT AL + + E R + ++NARYG C FNPPLP GFYGNA V PA S T K
Sbjct: 268 WRCRTLALQPENPNHEFRLLYILNARYGRCSFNPPLPEGFYGNAFV--SPAAIS--TGEK 323
Query: 476 LCSEPLEYALKLVKEAKGKATEEYVHSVVDFMAIKGRP--WFKSFGCFVVSDLSKAALSD 303
LC+ PLEYAL+L+KEAK K TEEYVHSV D M IKGRP ++ G VSDL+KA D
Sbjct: 324 LCNNPLEYALELMKEAKSKGTEEYVHSVADLMVIKGRPSYFYNDVGYLEVSDLTKARFRD 383
Query: 302 VDFGWGKAVYAGPPNILVTEASFFVPFTNSKGERGRVVIICLPGDAMERFEKELNDMLKI 123
VDFGWGKAVY G + + +V +TNSKG G + + LP AMERFEKEL+D+ K
Sbjct: 384 VDFGWGKAVYGGATQGYFS-SILYVSYTNSKGVEGIMALTSLPTKAMERFEKELDDLFKT 442
Query: 122 KN 117
K+
Sbjct: 443 KD 444
>gb|ABN48480.1| quinolizidine alkaloid O-tigloyltransferase [Lupinus albus]
Length = 453
Score = 501 bits (1291), Expect = e-139
Identities = 262/422 (62%), Positives = 310/422 (73%), Gaps = 2/422 (0%)
Frame = -3
Query: 1376 KLLSDIDDQNDMRFHMPIVHFYRYQPLMAGKDPVQLIREALAKTLVFYYPFAGRLKEGPK 1197
KLLSDIDDQ +R P+V YR P M GKDPV +IREAL+KTLVFYYPFAGRL+ GP
Sbjct: 31 KLLSDIDDQTSLRSLTPLVTIYRNNPSMEGKDPVAIIREALSKTLVFYYPFAGRLRNGPN 90
Query: 1196 GKLMVDCTGEGVMFIEADADVTLEQFGNDLLPPFPCFDELLYNVPGSEGLTGCPLLLIQV 1017
GKLMVDCTGEGV+FIEADADVTL+QFG DL PPFPCFD+LLY+VPGS+G+ PLLLIQV
Sbjct: 91 GKLMVDCTGEGVIFIEADADVTLDQFGIDLHPPFPCFDQLLYDVPGSDGILDSPLLLIQV 150
Query: 1016 TRLRCGGFIFAYRWNHTMSDASGISQFLNALAEIACGAPKPSILPVWCRELLCARDPPRV 837
TRL+CGGFIFA R NH M DA G+SQF+ LAEIA G PKP ILPVW RELLCAR+PP+V
Sbjct: 151 TRLKCGGFIFAVRLNHAMCDAIGMSQFMKGLAEIARGEPKPFILPVWXRELLCARNPPKV 210
Query: 836 TCAHDKEYQQELPVRNTKSILKLHHRSFFFGPTEIATIRALLPHHLAAQSTSFEVLTACI 657
T H+ EYQ+ P N + L H SFFFGP E+ IR LLP+H ++ST+ ++LTA +
Sbjct: 211 TFIHN-EYQKP-PHDNNNNNFILQHSSFFFGPNELDAIRRLLPYH-HSKSTTSDILTAFL 267
Query: 656 WQCRTKALHWQTRDEEIRFMGVVNARYGNCRFNPPLPIGFYGNASVVCLPAITSDTTVGK 477
W+CRT AL + + E R + ++NARYG C FNPPLP GFYGNA V PA S T K
Sbjct: 268 WRCRTLALQPENPNHEFRLLYILNARYGRCSFNPPLPEGFYGNAFV--SPAAIS--TGEK 323
Query: 476 LCSEPLEYALKLVKEAKGKATEEYVHSVVDFMAIKGRP--WFKSFGCFVVSDLSKAALSD 303
LC+ PLEYAL+L+KEAK K TEEYVHSV D M IKGRP ++ G VSDL+KA D
Sbjct: 324 LCNNPLEYALELMKEAKSKGTEEYVHSVADLMVIKGRPSYFYNDVGYLEVSDLTKARFRD 383
Query: 302 VDFGWGKAVYAGPPNILVTEASFFVPFTNSKGERGRVVIICLPGDAMERFEKELNDMLKI 123
VDFGWGKAVY G + + +V +TNSKG G + + LP AMERFEKEL+D+ K
Sbjct: 384 VDFGWGKAVYGGATQGYFS-SILYVSYTNSKGVEGIMALTSLPTKAMERFEKELDDLFKT 442
Query: 122 KN 117
K+
Sbjct: 443 KD 444