BLASTX nr result
ID: Glycyrrhiza23_contig00013775
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Glycyrrhiza23_contig00013775
(1312 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_003549135.1| PREDICTED: 13-hydroxylupanine O-tigloyltrans... 471 e-167
sp|Q5H873.1|HLTT_LUPAL RecName: Full=13-hydroxylupanine O-tigloy... 412 e-141
gb|ABN48480.1| quinolizidine alkaloid O-tigloyltransferase [Lupi... 407 e-139
gb|ACJ85557.1| unknown [Medicago truncatula] 393 e-137
gb|AFK36385.1| unknown [Medicago truncatula] 393 e-137
>ref|XP_003549135.1| PREDICTED: 13-hydroxylupanine O-tigloyltransferase-like [Glycine max]
Length = 455
Score = 471 bits (1213), Expect(2) = e-167
Identities = 225/297 (75%), Positives = 259/297 (87%), Gaps = 1/297 (0%)
Frame = -1
Query: 1228 ELKLLSDLDDQNGLRAHVPIVHFYRYQPLMAGKDTVQVIREALAKTLVFYYPFAGRLREG 1049
E+KLLSD+DDQNGLR +P+V F+ YQP M GKD V+VIREAL+KTLVFYYPFAGRLREG
Sbjct: 29 EVKLLSDIDDQNGLRYQLPLVLFFPYQPSMEGKDPVEVIREALSKTLVFYYPFAGRLREG 88
Query: 1048 PKGKLMVDCTGEGVMFIEADADVTLEQFGSDLLPPFPCFDELLYNVPGSDGMIDCPLLLI 869
P GKLMVDC GEGVMFIEADADVT+EQFG++ +PPFPCFDELLYNVPGSDGMID PLLLI
Sbjct: 89 PDGKLMVDCNGEGVMFIEADADVTIEQFGNNFMPPFPCFDELLYNVPGSDGMIDTPLLLI 148
Query: 868 QVTRLKCGGFIFAFRLNHTMTDASGLSQFLKALAEIALGAPKPSTLPVWHRELLCARDPP 689
QVTRLKCGGFIFA R+NHTM D SG+ QFLKAL+EIA GAPKPS LP WHRE+LCAR+PP
Sbjct: 149 QVTRLKCGGFIFALRMNHTMCDGSGICQFLKALSEIAHGAPKPSILPGWHREILCAREPP 208
Query: 688 RVTCIHREYQRQSLPNTKSIFEPHQRSFFFGPIEIATIRALLPHHLAHQSTSYEVLTAYL 509
R+TCIH+EYQ Q P+++SIF PHQRSFFFGP EIA++RALLPHHLA +STS+EV+TA L
Sbjct: 209 RITCIHQEYQ-QLPPDSRSIFIPHQRSFFFGPKEIASLRALLPHHLATKSTSFEVITACL 267
Query: 508 WRCRAKALYWQNPDQEVSLLGIINVNYGH-GFNPPLPNGFYGNTIVLPAAITTAGEL 341
WRCR +L WQNP+QEV LL I+N +G+ FNPPLP+GFYGN V PAA+TT G+L
Sbjct: 268 WRCRTASLKWQNPNQEVRLLCIVNARFGNCRFNPPLPDGFYGNAFVFPAAVTTVGKL 324
Score = 146 bits (368), Expect(2) = e-167
Identities = 74/111 (66%), Positives = 91/111 (81%), Gaps = 1/111 (0%)
Frame = -3
Query: 332 TLGYALELVKKAKDEATEEYVRSVIDLMVIKGRPSCITDIGTFMVSDLSKAAFGDVDFGW 153
+LGYA+ELVKKAKDEA EEYV SV DLM IKGRP C T +G+FMVSDL+K+ DV+ GW
Sbjct: 328 SLGYAVELVKKAKDEADEEYVHSVADLMAIKGRP-CFTKLGSFMVSDLTKSGLIDVNLGW 386
Query: 152 GKAVYAGLPK-CIGENNELSHYVPFTNSKGEQGRVVLICLPVDAMKRFEKE 3
GKA+Y+G+ K +G+ +S YVP+TNSKGE+GRV+ ICLP DAM+RFEKE
Sbjct: 387 GKALYSGVAKGGLGDIPGVSFYVPYTNSKGERGRVIPICLPEDAMERFEKE 437
>sp|Q5H873.1|HLTT_LUPAL RecName: Full=13-hydroxylupanine O-tigloyltransferase; AltName:
Full=(-)-13alpha-hydroxymultiflorine/(+)-13alpha-
hydroxylupanine O-tigloyltransferase; Short=HMT/HLTase;
AltName: Full=Quinolizidine alkaloid O-tigloyltransferase
gi|58530624|dbj|BAD89275.1|
(-)-13alpha-hydroxymultiflorine/(+)-13alpha-
hydroxylupanine O-tigloyltransferase [Lupinus albus]
Length = 453
Score = 412 bits (1058), Expect(2) = e-141
Identities = 202/302 (66%), Positives = 235/302 (77%), Gaps = 1/302 (0%)
Frame = -1
Query: 1231 RELKLLSDLDDQNGLRAHVPIVHFYRYQPLMAGKDTVQVIREALAKTLVFYYPFAGRLRE 1052
+E KLLSD+DDQ LR+ P+V YR P M GKD V++IREAL+KTLVFYYPFAGRLR
Sbjct: 28 KEFKLLSDIDDQTSLRSLTPLVTIYRNNPSMEGKDPVEIIREALSKTLVFYYPFAGRLRN 87
Query: 1051 GPKGKLMVDCTGEGVMFIEADADVTLEQFGSDLLPPFPCFDELLYNVPGSDGMIDCPLLL 872
GP GKLMVDCTGEGV+FIEADADVTL+QFG DL PPFPCFD+LLY+VPGSDG++D PLLL
Sbjct: 88 GPNGKLMVDCTGEGVIFIEADADVTLDQFGIDLHPPFPCFDQLLYDVPGSDGILDSPLLL 147
Query: 871 IQVTRLKCGGFIFAFRLNHTMTDASGLSQFLKALAEIALGAPKPSTLPVWHRELLCARDP 692
IQVTRLKCGGFIFA RLNH M DA G+SQF+K LAEIA G PKP LPVWHRELLCAR+P
Sbjct: 148 IQVTRLKCGGFIFAVRLNHAMCDAIGMSQFMKGLAEIARGEPKPFILPVWHRELLCARNP 207
Query: 691 PRVTCIHREYQRQSLPNTKSIFEPHQRSFFFGPIEIATIRALLPHHLAHQSTSYEVLTAY 512
P+VT IH EYQ+ N + F SFFFGP E+ IR LLP+H + +ST+ ++LTA+
Sbjct: 208 PKVTFIHNEYQKPPHDNNNNNFILQHSSFFFGPNELDAIRRLLPYHHS-KSTTSDILTAF 266
Query: 511 LWRCRAKALYWQNPDQEVSLLGIINVNYGH-GFNPPLPNGFYGNTIVLPAAITTAGELCS 335
LWRCR AL +NP+ E LL I+N YG FNPPLP GFYGN V PAAI+T +LC+
Sbjct: 267 LWRCRTLALQPENPNHEFRLLYILNARYGRCSFNPPLPEGFYGNAFVSPAAISTGEKLCN 326
Query: 334 QP 329
P
Sbjct: 327 NP 328
Score = 117 bits (292), Expect(2) = e-141
Identities = 66/110 (60%), Positives = 78/110 (70%), Gaps = 1/110 (0%)
Frame = -3
Query: 329 LGYALELVKKAKDEATEEYVRSVIDLMVIKGRPSCI-TDIGTFMVSDLSKAAFGDVDFGW 153
L YALEL+K+AK + TEEYV SV DLMVIKGRPS D+G VSDL+KA F DVDFGW
Sbjct: 329 LEYALELMKEAKSKGTEEYVHSVADLMVIKGRPSYFYNDVGYLEVSDLTKARFRDVDFGW 388
Query: 152 GKAVYAGLPKCIGENNELSHYVPFTNSKGEQGRVVLICLPVDAMKRFEKE 3
GKAVY G + G + + YV +TNSKG +G + L LP AM+RFEKE
Sbjct: 389 GKAVYGGATQ--GYFSSIL-YVSYTNSKGVEGIMALTSLPTKAMERFEKE 435
>gb|ABN48480.1| quinolizidine alkaloid O-tigloyltransferase [Lupinus albus]
Length = 453
Score = 407 bits (1046), Expect(2) = e-139
Identities = 201/302 (66%), Positives = 233/302 (77%), Gaps = 1/302 (0%)
Frame = -1
Query: 1231 RELKLLSDLDDQNGLRAHVPIVHFYRYQPLMAGKDTVQVIREALAKTLVFYYPFAGRLRE 1052
+E KLLSD+DDQ LR+ P+V YR P M GKD V +IREAL+KTLVFYYPFAGRLR
Sbjct: 28 KEFKLLSDIDDQTSLRSLTPLVTIYRNNPSMEGKDPVAIIREALSKTLVFYYPFAGRLRN 87
Query: 1051 GPKGKLMVDCTGEGVMFIEADADVTLEQFGSDLLPPFPCFDELLYNVPGSDGMIDCPLLL 872
GP GKLMVDCTGEGV+FIEADADVTL+QFG DL PPFPCFD+LLY+VPGSDG++D PLLL
Sbjct: 88 GPNGKLMVDCTGEGVIFIEADADVTLDQFGIDLHPPFPCFDQLLYDVPGSDGILDSPLLL 147
Query: 871 IQVTRLKCGGFIFAFRLNHTMTDASGLSQFLKALAEIALGAPKPSTLPVWHRELLCARDP 692
IQVTRLKCGGFIFA RLNH M DA G+SQF+K LAEIA G PKP LPVW RELLCAR+P
Sbjct: 148 IQVTRLKCGGFIFAVRLNHAMCDAIGMSQFMKGLAEIARGEPKPFILPVWXRELLCARNP 207
Query: 691 PRVTCIHREYQRQSLPNTKSIFEPHQRSFFFGPIEIATIRALLPHHLAHQSTSYEVLTAY 512
P+VT IH EYQ+ N + F SFFFGP E+ IR LLP+H + +ST+ ++LTA+
Sbjct: 208 PKVTFIHNEYQKPPHDNNNNNFILQHSSFFFGPNELDAIRRLLPYHHS-KSTTSDILTAF 266
Query: 511 LWRCRAKALYWQNPDQEVSLLGIINVNYGH-GFNPPLPNGFYGNTIVLPAAITTAGELCS 335
LWRCR AL +NP+ E LL I+N YG FNPPLP GFYGN V PAAI+T +LC+
Sbjct: 267 LWRCRTLALQPENPNHEFRLLYILNARYGRCSFNPPLPEGFYGNAFVSPAAISTGEKLCN 326
Query: 334 QP 329
P
Sbjct: 327 NP 328
Score = 117 bits (292), Expect(2) = e-139
Identities = 66/110 (60%), Positives = 78/110 (70%), Gaps = 1/110 (0%)
Frame = -3
Query: 329 LGYALELVKKAKDEATEEYVRSVIDLMVIKGRPSCI-TDIGTFMVSDLSKAAFGDVDFGW 153
L YALEL+K+AK + TEEYV SV DLMVIKGRPS D+G VSDL+KA F DVDFGW
Sbjct: 329 LEYALELMKEAKSKGTEEYVHSVADLMVIKGRPSYFYNDVGYLEVSDLTKARFRDVDFGW 388
Query: 152 GKAVYAGLPKCIGENNELSHYVPFTNSKGEQGRVVLICLPVDAMKRFEKE 3
GKAVY G + G + + YV +TNSKG +G + L LP AM+RFEKE
Sbjct: 389 GKAVYGGATQ--GYFSSIL-YVSYTNSKGVEGIMALTSLPTKAMERFEKE 435
>gb|ACJ85557.1| unknown [Medicago truncatula]
Length = 459
Score = 393 bits (1009), Expect(2) = e-137
Identities = 195/304 (64%), Positives = 235/304 (77%), Gaps = 4/304 (1%)
Frame = -1
Query: 1228 ELKLLSDLDDQNGLRAHVPIVHFYRYQPLMAGKDTVQVIREALAKTLVFYYPFAGRLREG 1049
E+KLLSD+DDQ GLR +P++ FY+Y P MAGKD V +IR+A AKTLVFYYPFAGRLREG
Sbjct: 26 EIKLLSDIDDQEGLRFQIPVIQFYKYDPNMAGKDPVDIIRKASAKTLVFYYPFAGRLREG 85
Query: 1048 PKGKLMVDCTGEGVMFIEADADVTLEQFGSDLLPPFPCFDELLYNVPGSDGMIDCPLLLI 869
P KLMVDCTGEGV+FIEADADV+L++FG L PPFPC DELL++VPGS +++CPLL+I
Sbjct: 86 PGRKLMVDCTGEGVLFIEADADVSLKEFGDALQPPFPCLDELLFDVPGSSEVLNCPLLVI 145
Query: 868 QVTRLKCGGFIFAFRLNHTMTDASGLSQFLKALAEIALGAPKPSTLPVWHRELLCARDPP 689
QVTRLKCGGFIFA RLNHTM+DA+GL QF+ AL EI+ G +PS LPVWHRELL +RDPP
Sbjct: 146 QVTRLKCGGFIFALRLNHTMSDAAGLVQFMSALGEISCGMNEPSILPVWHRELLNSRDPP 205
Query: 688 RVTCIHREYQRQSLPNTKSIFEP----HQRSFFFGPIEIATIRALLPHHLAHQSTSYEVL 521
RVTC HREY++ +P+TK P RSFFFGP E+A IRALLP H QS ++E+L
Sbjct: 206 RVTCTHREYEQ--VPDTKGTIIPLDDMAHRSFFFGPTEVAKIRALLPPHQQKQS-NFEIL 262
Query: 520 TAYLWRCRAKALYWQNPDQEVSLLGIINVNYGHGFNPPLPNGFYGNTIVLPAAITTAGEL 341
TA LWRCR AL + D+EV ++ I+N FNPPLPNG+YGN P A+TTAG+L
Sbjct: 263 TACLWRCRTIALQ-PDTDEEVRIICIVNAR--GKFNPPLPNGYYGNAFAFPVAVTTAGKL 319
Query: 340 CSQP 329
P
Sbjct: 320 MENP 323
Score = 124 bits (312), Expect(2) = e-137
Identities = 64/111 (57%), Positives = 83/111 (74%), Gaps = 2/111 (1%)
Frame = -3
Query: 329 LGYALELVKKAKDEATEEYVRSVIDLMVIKGRPSCITDIGTFMVSDLSKAAFGDVDFGWG 150
LGYALELVKKAK + T+EY+ SV DLMVIKGRP T + +++VSD+++A FGDV+FGWG
Sbjct: 324 LGYALELVKKAKSDVTQEYMHSVADLMVIKGRPH-FTVVRSYLVSDVTRAGFGDVEFGWG 382
Query: 149 KAVYAGLPK-CIGE-NNELSHYVPFTNSKGEQGRVVLICLPVDAMKRFEKE 3
KAVY G K +G S Y+PF N+KGE+G V+ +CLP AM++F KE
Sbjct: 383 KAVYGGPAKGGVGAIPGVASFYIPFKNAKGEEGLVIPLCLPAQAMEKFVKE 433
>gb|AFK36385.1| unknown [Medicago truncatula]
Length = 459
Score = 393 bits (1009), Expect(2) = e-137
Identities = 195/304 (64%), Positives = 235/304 (77%), Gaps = 4/304 (1%)
Frame = -1
Query: 1228 ELKLLSDLDDQNGLRAHVPIVHFYRYQPLMAGKDTVQVIREALAKTLVFYYPFAGRLREG 1049
E+KLLSD+DDQ GLR +P++ FY+Y P MAGKD V +IR+A AKTLVFYYPFAGRLREG
Sbjct: 26 EIKLLSDIDDQEGLRFQIPVIQFYKYDPNMAGKDPVDIIRKASAKTLVFYYPFAGRLREG 85
Query: 1048 PKGKLMVDCTGEGVMFIEADADVTLEQFGSDLLPPFPCFDELLYNVPGSDGMIDCPLLLI 869
P KLMVDCTGEGV+FIEADADV+L++FG L PPFPC DELL++VPGS +++CPLL+I
Sbjct: 86 PGRKLMVDCTGEGVLFIEADADVSLKEFGDALQPPFPCLDELLFDVPGSSEVLNCPLLVI 145
Query: 868 QVTRLKCGGFIFAFRLNHTMTDASGLSQFLKALAEIALGAPKPSTLPVWHRELLCARDPP 689
QVTRLKCGGFIFA RLNHTM+DA+GL QF+ AL EI+ G +PS LPVWHRELL +RDPP
Sbjct: 146 QVTRLKCGGFIFALRLNHTMSDAAGLVQFMSALGEISCGMNEPSILPVWHRELLNSRDPP 205
Query: 688 RVTCIHREYQRQSLPNTKSIFEP----HQRSFFFGPIEIATIRALLPHHLAHQSTSYEVL 521
RVTC HREY++ +P+TK P RSFFFGP E+A IRALLP H QS ++E+L
Sbjct: 206 RVTCTHREYEQ--VPDTKGTIIPLDDMAHRSFFFGPTEVAKIRALLPPHQQKQS-NFEIL 262
Query: 520 TAYLWRCRAKALYWQNPDQEVSLLGIINVNYGHGFNPPLPNGFYGNTIVLPAAITTAGEL 341
TA LWRCR AL + D+EV ++ I+N FNPPLPNG+YGN P A+TTAG+L
Sbjct: 263 TACLWRCRTIALQ-PDTDEEVRIICIVNAR--GKFNPPLPNGYYGNAFAFPVAVTTAGKL 319
Query: 340 CSQP 329
P
Sbjct: 320 MENP 323
Score = 122 bits (306), Expect(2) = e-137
Identities = 63/111 (56%), Positives = 82/111 (73%), Gaps = 2/111 (1%)
Frame = -3
Query: 329 LGYALELVKKAKDEATEEYVRSVIDLMVIKGRPSCITDIGTFMVSDLSKAAFGDVDFGWG 150
LGYALELVKKAK + T+EY+ SV DLMVIKGRP T + +++V D+++A FGDV+FGWG
Sbjct: 324 LGYALELVKKAKSDVTQEYMHSVADLMVIKGRPH-FTVVRSYLVFDVTRAGFGDVEFGWG 382
Query: 149 KAVYAGLPK-CIGE-NNELSHYVPFTNSKGEQGRVVLICLPVDAMKRFEKE 3
KAVY G K +G S Y+PF N+KGE+G V+ +CLP AM++F KE
Sbjct: 383 KAVYGGPAKGGVGAIPGVASFYIPFKNAKGEEGLVIPLCLPAQAMEKFVKE 433