BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Glycyrrhiza23_contig00013629
         (2966 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AAZ81836.1| isoamylase isoform 2 [Pisum sativum]                  1223   0.0  
ref|XP_003554240.1| PREDICTED: isoamylase 2, chloroplastic-like ...  1201   0.0  
ref|XP_003520557.1| PREDICTED: isoamylase 2, chloroplastic-like ...  1171   0.0  
dbj|BAF52942.1| isoamylase-type starch-debranching enzyme 2 [Pha...  1155   0.0  
ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Viti...   969   0.0  

>gb|AAZ81836.1| isoamylase isoform 2 [Pisum sativum]
          Length = 857

 Score = 1223 bits (3164), Expect = 0.0
 Identities = 633/861 (73%), Positives = 695/861 (80%), Gaps = 11/861 (1%)
 Frame = -2

Query: 2767 MANLVPSLSFSVIPWNQQHKHGTKCPFFHNYSFTQRKQQHQLVSRNLQDLTNPFSQNLTT 2588
            M NL+P+ SFSV P    HK+ TKC FFH  SFT  + Q+QLVS+NLQDL  PFSQNLT+
Sbjct: 1    MTNLLPAFSFSVTP----HKYETKCSFFHT-SFTCIQHQNQLVSKNLQDLKIPFSQNLTS 55

Query: 2587 KLCATS-RLSVEETEQKITPFTQSEDLKTALPYLFRTETGDGLXXXXXXXXXXXXXXYIE 2411
            KLCATS RLS+EET QK+  FTQSED+KTALPYLFRTETG+GL              YIE
Sbjct: 56   KLCATSSRLSIEETGQKLISFTQSEDIKTALPYLFRTETGEGLVKVYVKKKKDTYFVYIE 115

Query: 2410 ISSLELSGGEGEALVLCWGVYRVDSSSV---EAMNVTPLVQTSQGKFAVELEFDARQVPL 2240
            +SSLELS  EGE  VLCWGVY  DSSS+   + MNV+PLV+ S GKF+VELEFD  QVPL
Sbjct: 116  VSSLELSNVEGETFVLCWGVYSDDSSSLGSGKGMNVSPLVKNSLGKFSVELEFDVEQVPL 175

Query: 2239 YLSFLLR-SSLEIRSHRKRNFCVPVXXXXXXXXXXXXXXXXXXSMNFAVFSRHAE-GVVL 2066
            YLSFLLR S  EI +H ++ F                       ++   F R  + GVVL
Sbjct: 176  YLSFLLRLSGSEITTHAEKKFLRACLVFLGVVHLRWGSPFLLTGLSILQFFRGMQQGVVL 235

Query: 2065 CLYDDD-DTSVEEKKPALELDLDPYVNKSGDIWHISFESALNFVSYGYRFLHTNKDD--- 1898
            CLYD++ DT V  KKPALELDLDPYVN+SGDIWHIS E+A +FVSY YRF   N+D+   
Sbjct: 236  CLYDNNVDTGV--KKPALELDLDPYVNRSGDIWHISLENAKSFVSYCYRFRGANRDNSYA 293

Query: 1897 KGVVLDPYARIVGNSLPNGVGSVKKNLGCLGKEPAFDWGDDYHPNLAMEELVVYRLNVKR 1718
            + VVLDPYARIVGNS PNG+GSVK NLG L KEPAFDWGDDYH NL ME+LVVYRLNVK 
Sbjct: 294  ECVVLDPYARIVGNSFPNGIGSVK-NLGFLRKEPAFDWGDDYHLNLDMEKLVVYRLNVKH 352

Query: 1717 FTEHESSQLSSDLAGTFSGLAKKLQHFKDLGVNAVLLEPVFTFDEEKGAYFPCHFFSPMN 1538
            FTEHESSQLS DLAGTFSGLAKKLQHFKDLGVNAVLLEPVFTFDEEKG YFPCHFFSP+N
Sbjct: 353  FTEHESSQLSGDLAGTFSGLAKKLQHFKDLGVNAVLLEPVFTFDEEKGPYFPCHFFSPVN 412

Query: 1537 LYGPSGGPVSTVTSMKEMVKTMHANGIEVLIEVVFSNTADTGALQGIDDLSYYYANGV-G 1361
            LYGPSG P ST+ SMKEMVKTMHANGIEV++EVVFSNTA+ GALQGIDDLSYYYANGV G
Sbjct: 413  LYGPSGDPESTINSMKEMVKTMHANGIEVIMEVVFSNTAEVGALQGIDDLSYYYANGVVG 472

Query: 1360 DLKVHSALNCNYPIVQNLILDSLRHWVTEFHIDGFSFVNASHLLRGFHGEHLSRPPSVEA 1181
             LKVHSALNCNYPIVQNLILDSLRHWVTEFHIDGFSFVNASHLLRG+HGE+LSRPP VEA
Sbjct: 473  GLKVHSALNCNYPIVQNLILDSLRHWVTEFHIDGFSFVNASHLLRGYHGEYLSRPPLVEA 532

Query: 1180 IAFDPVLWKTKIIADCWDPHDMVAKETRFPHWMRWAEINTNFCNDVRNFLRGENLLSNLA 1001
            IAFDPVLWKTKIIADCWDP+DM  KE RFPHWMRWAEINTNF +DVRNFLRGE+LLSNLA
Sbjct: 533  IAFDPVLWKTKIIADCWDPNDMETKEIRFPHWMRWAEINTNFRSDVRNFLRGESLLSNLA 592

Query: 1000 TRLCGSGDMFSDGRGPAFSLNYIARNFGLSLVDIVSFSSDDVAAELSWNCGEEGPTNNRT 821
            TRLCGSGDM+SDGRGPAFS NYI+ NFGLSLVD+VSFS+  + AELSWNCGEEGPTN+  
Sbjct: 593  TRLCGSGDMYSDGRGPAFSFNYISGNFGLSLVDLVSFSNAGLEAELSWNCGEEGPTNSTA 652

Query: 820  VLERRLKQIRNFLFILFMSLGVPVLNMGDECGQSSDGSPAYGDMKPFNWVTLKTGFGKQT 641
            VLERRLKQIRNFLFIL++SLGVP+LNMGDECG SS GS A G+ KPFNW  LKTGFGKQT
Sbjct: 653  VLERRLKQIRNFLFILYVSLGVPILNMGDECGHSSGGSLANGNTKPFNWAALKTGFGKQT 712

Query: 640  TQFIXXXXXXXXXXXXXXXXXXXLKEENIEWHGADQAPPRWEDPSCKFLAMTLKAEEREF 461
             QFI                   LKEENIEW G D+APP+WEDPSCKFLAMTLKAE  E 
Sbjct: 713  IQFISFLTSLRTRRSDLLQSRSFLKEENIEWRGIDEAPPKWEDPSCKFLAMTLKAERNEH 772

Query: 460  QESSSVSSDILGDLFIAFNAADHPETVVLPFPPEGMSWYRLVDTALPFPGFFLTNGELVP 281
            QE SSVSSDILGDLF+AFNA D PETVVLP PPEGMSWYR++DTALPFPGFFL NG+LV 
Sbjct: 773  QE-SSVSSDILGDLFVAFNADDCPETVVLPLPPEGMSWYRIIDTALPFPGFFLNNGDLVL 831

Query: 280  EEIPGLSTYQMKPYSCTLFEA 218
            E++ GLSTY+MK YSC LFEA
Sbjct: 832  EQMSGLSTYEMKSYSCILFEA 852


>ref|XP_003554240.1| PREDICTED: isoamylase 2, chloroplastic-like [Glycine max]
          Length = 865

 Score = 1201 bits (3107), Expect = 0.0
 Identities = 628/880 (71%), Positives = 689/880 (78%), Gaps = 26/880 (2%)
 Frame = -2

Query: 2767 MANLVPSLSFSVIPWNQQHKHGTKCPFFHNYSFTQRKQQ-HQLVSRNLQDLTNPFSQNLT 2591
            MA L P  SFSVIP N    H  K  F H   F QRKQ  HQL    LQ L +PFS N T
Sbjct: 1    MAILAPRFSFSVIPRNH---HKLKRSFSHK-PFIQRKQLWHQL---GLQSLVSPFSLNPT 53

Query: 2590 T-KLCATSRLSVEETEQKITPFTQSEDLKTALPYLFRTETGDGLXXXXXXXXXXXXXXYI 2414
            + KLCATSRLS+EETEQ+I   T+ EDLK +L YLFRTETG GL              YI
Sbjct: 54   SSKLCATSRLSIEETEQQIGTLTRPEDLKGSLAYLFRTETGGGLVKVHVTRRNDRYSVYI 113

Query: 2413 EISSLELSGGEGEALVLCWGVYRVDSS-------------SVEAMNVTPLVQTSQGKFAV 2273
            EISSL++SGG GEAL+LCWGVYR DSS             +   MNV+PLVQ S GKFA+
Sbjct: 114  EISSLDISGGVGEALLLCWGVYRSDSSCFVDLDTIGLSENAAMGMNVSPLVQNSDGKFAI 173

Query: 2272 ELEFDARQVPLYLSFLLRSSL----EIRSHRKRNFCVPVXXXXXXXXXXXXXXXXXXSMN 2105
            ELEFDA+ VPLYLSF L SSL    EIRSHR+ NFC+PV                  S+N
Sbjct: 174  ELEFDAKHVPLYLSFFLMSSLDSGLEIRSHRRTNFCMPVGSLPGYPCPLGVSYSPDGSVN 233

Query: 2104 FAVFSRHAEGVVLCLYDDDDTSVEEKKPALELDLDPYVNKSGDIWHISFESALNFVSYGY 1925
            F++FSRHAE VVLCLYD++      +KPALELDLDPYVN++GDIWH+SFESA  FVSYGY
Sbjct: 234  FSIFSRHAESVVLCLYDENGV----EKPALELDLDPYVNRTGDIWHVSFESAKGFVSYGY 289

Query: 1924 R----FLHTNKDD---KGVVLDPYARIVGNSLPNGVGSVKKNLGCLGKEPAFDWGDDYHP 1766
            R     L  NKDD   + VVLDPYA+IVGNS P+GVG VK NLGCL KEP FDWG D HP
Sbjct: 290  RCRRGVLKKNKDDGFAEHVVLDPYAKIVGNSYPDGVGLVK-NLGCLRKEPFFDWGGDRHP 348

Query: 1765 NLAMEELVVYRLNVKRFTEHESSQLSSDLAGTFSGLAKKLQHFKDLGVNAVLLEPVFTFD 1586
            +L+ME+LVVYRLNVKRFT+HESSQL S LAGTF+GLAKK+QHFKDLGVNAVLLEPVFTFD
Sbjct: 349  DLSMEKLVVYRLNVKRFTQHESSQLPSGLAGTFTGLAKKVQHFKDLGVNAVLLEPVFTFD 408

Query: 1585 EEKGAYFPCHFFSPMNLYGPSGGPVSTVTSMKEMVKTMHANGIEVLIEVVFSNTADTGAL 1406
            E+KG YFPCHFFS M++YGPSGGPVS + +MKEMVKTMHANGIEVL+EVVFSNTA+ GA+
Sbjct: 409  EKKGPYFPCHFFSLMHIYGPSGGPVSAIAAMKEMVKTMHANGIEVLVEVVFSNTAEIGAI 468

Query: 1405 QGIDDLSYYYANGVGDLKVHSALNCNYPIVQNLILDSLRHWVTEFHIDGFSFVNASHLLR 1226
            QGIDD SYYYANGVG LKV SALNCNYPIVQNLILDSLRHWVTEFHIDGFSF+NASHLLR
Sbjct: 469  QGIDDSSYYYANGVGGLKVQSALNCNYPIVQNLILDSLRHWVTEFHIDGFSFINASHLLR 528

Query: 1225 GFHGEHLSRPPSVEAIAFDPVLWKTKIIADCWDPHDMVAKETRFPHWMRWAEINTNFCND 1046
            GFHGE+LSRPP VEAIAFDPVL KTKIIADCWDPH MVAKE RFPHWMRWAE+NT+FCND
Sbjct: 529  GFHGEYLSRPPLVEAIAFDPVLSKTKIIADCWDPHGMVAKEIRFPHWMRWAEMNTHFCND 588

Query: 1045 VRNFLRGENLLSNLATRLCGSGDMFSDGRGPAFSLNYIARNFGLSLVDIVSFSSDDVAAE 866
            VRNFLRG+NLLS+LATRLCGSGD+FS GRGP FS NYIARNFG+SLVD+VSFSS D   E
Sbjct: 589  VRNFLRGQNLLSDLATRLCGSGDIFSGGRGPGFSFNYIARNFGVSLVDLVSFSSVD---E 645

Query: 865  LSWNCGEEGPTNNRTVLERRLKQIRNFLFILFMSLGVPVLNMGDECGQSSDGSPAYGDMK 686
            LSWNCG EGPTNN  VLERRLKQIRNFLFILF+SLGVPVLNMGDECGQSS G  AY  +K
Sbjct: 646  LSWNCGAEGPTNNTAVLERRLKQIRNFLFILFVSLGVPVLNMGDECGQSSGGFTAYDGIK 705

Query: 685  PFNWVTLKTGFGKQTTQFIXXXXXXXXXXXXXXXXXXXLKEENIEWHGADQAPPRWEDPS 506
            PF+W  LKTGFGKQT++FI                   LKEENIEW+G+D  PPRWEDPS
Sbjct: 706  PFSWSALKTGFGKQTSEFIFFLSSLRKRRSYLLQRRSFLKEENIEWYGSDGDPPRWEDPS 765

Query: 505  CKFLAMTLKAEEREFQESSSVSSDILGDLFIAFNAADHPETVVLPFPPEGMSWYRLVDTA 326
            CKFLAM LKAE  EF E SSVSSDI GDLFIAFNA DHPET VLP PPEGMSWYRLVDTA
Sbjct: 766  CKFLAMILKAEVTEFLE-SSVSSDISGDLFIAFNATDHPETAVLPLPPEGMSWYRLVDTA 824

Query: 325  LPFPGFFLTNGELVPEEIPGLSTYQMKPYSCTLFEAKNHT 206
            LPFPGFF T+GE+VPE+  GL TYQ+K YSCTLFEA N T
Sbjct: 825  LPFPGFFSTSGEVVPEQTEGLFTYQVKSYSCTLFEANNRT 864


>ref|XP_003520557.1| PREDICTED: isoamylase 2, chloroplastic-like [Glycine max]
          Length = 866

 Score = 1171 bits (3029), Expect = 0.0
 Identities = 614/879 (69%), Positives = 679/879 (77%), Gaps = 25/879 (2%)
 Frame = -2

Query: 2767 MANLVPSLSFSVIPWNQQHKHGTKCPFFHNYSFTQRKQQHQLVSRNLQDLTNPFSQNLTT 2588
            M +L P  SFSVIP +   K    C  FH   F QRK  H      LQ+  +PFS+N T+
Sbjct: 1    MTSLAPRFSFSVIPGSHHSKPKFSC--FHE-PFIQRK--HLWPKLGLQNPISPFSRNPTS 55

Query: 2587 -KLCATSRLSVEETEQKITPFTQSEDLKTALPYLFRTETGDGLXXXXXXXXXXXXXXYIE 2411
             KLCATSRLS+EETEQ++   ++ E LK +  YLFRTETG GL              Y E
Sbjct: 56   SKLCATSRLSIEETEQQLGTLSRPEYLKGSSAYLFRTETGGGLVKVYVTKRKDRYFVYTE 115

Query: 2410 ISSLELSGGEGEALVLCWGVYRVDSS-------------SVEAMNVTPLVQTSQGKFAVE 2270
            ISSL++SG  GE L+LCWGVYR DSS             +   MNV+PLVQ S G FAVE
Sbjct: 116  ISSLDISGSVGETLLLCWGVYRSDSSCFVDLDTIGLRENAATGMNVSPLVQNSDGNFAVE 175

Query: 2269 LEFDARQVPLYLSFLLRSSL----EIRSHRKRNFCVPVXXXXXXXXXXXXXXXXXXSMNF 2102
            LEFDA+ VPLYLSF L SSL    EIRSHR  NFCVPV                  S+NF
Sbjct: 176  LEFDAKHVPLYLSFFLMSSLDAGMEIRSHRGTNFCVPVGLLPGYPGPLGLFCSPDGSVNF 235

Query: 2101 AVFSRHAEGVVLCLYDDDDTSVEEKKPALELDLDPYVNKSGDIWHISFESALNFVSYGYR 1922
            A+FSR AE VVLCLYD++D     +KPALELDLDPYVN++GDIWH++FESA  F+SYGY 
Sbjct: 236  AIFSRRAESVVLCLYDENDM----EKPALELDLDPYVNRTGDIWHVAFESAKGFMSYGYS 291

Query: 1921 ----FLHTNKDD---KGVVLDPYARIVGNSLPNGVGSVKKNLGCLGKEPAFDWGDDYHPN 1763
                 L  NKDD   + VVLDPYA+IVGNS P+GVG VK NLG LGKEP FDWG D H +
Sbjct: 292  CRGGVLKRNKDDGFAEHVVLDPYAKIVGNSYPDGVGFVK-NLGWLGKEPDFDWGGDCHLD 350

Query: 1762 LAMEELVVYRLNVKRFTEHESSQLSSDLAGTFSGLAKKLQHFKDLGVNAVLLEPVFTFDE 1583
            L+ME+LVVYRLNVKRFT+HESSQL S LAGTF+GLAKK+QHFKDLGVNA+LLEPVFTFDE
Sbjct: 351  LSMEKLVVYRLNVKRFTQHESSQLPSGLAGTFTGLAKKVQHFKDLGVNAILLEPVFTFDE 410

Query: 1582 EKGAYFPCHFFSPMNLYGPSGGPVSTVTSMKEMVKTMHANGIEVLIEVVFSNTADTGALQ 1403
            +KG YFP HFFS M++YGPSGGPVS + SMKEMVKTMHANGIEVL+EVVFSNTA+ GALQ
Sbjct: 411  KKGPYFPSHFFSLMHIYGPSGGPVSAIASMKEMVKTMHANGIEVLVEVVFSNTAEIGALQ 470

Query: 1402 GIDDLSYYYANGVGDLKVHSALNCNYPIVQNLILDSLRHWVTEFHIDGFSFVNASHLLRG 1223
            GIDD +YYYANGVG LK  SALNCNYPIVQ+LILDSLRHWVTEFHIDGFSF+NASHLLRG
Sbjct: 471  GIDDSTYYYANGVGGLKGQSALNCNYPIVQSLILDSLRHWVTEFHIDGFSFLNASHLLRG 530

Query: 1222 FHGEHLSRPPSVEAIAFDPVLWKTKIIADCWDPHDMVAKETRFPHWMRWAEINTNFCNDV 1043
            FHGE+L+RPP VEAIAFDPVL KTKIIADCWDPH MVAKE RFPHWMRWAEINTNFCNDV
Sbjct: 531  FHGEYLTRPPLVEAIAFDPVLSKTKIIADCWDPHGMVAKEIRFPHWMRWAEINTNFCNDV 590

Query: 1042 RNFLRGENLLSNLATRLCGSGDMFSDGRGPAFSLNYIARNFGLSLVDIVSFSSDDVAAEL 863
            RNFLRGENLLSNLATRLCGSGD+FS GRGPAFS NYIARNFG+SLVD+VSFSS D   EL
Sbjct: 591  RNFLRGENLLSNLATRLCGSGDIFSGGRGPAFSFNYIARNFGVSLVDLVSFSSTD---EL 647

Query: 862  SWNCGEEGPTNNRTVLERRLKQIRNFLFILFMSLGVPVLNMGDECGQSSDGSPAYGDMKP 683
            SWNCGEEGPTNN  +LERRLKQIRNFLFILF+SLGVPVLNMGDECGQSS G  AY  +KP
Sbjct: 648  SWNCGEEGPTNNTAILERRLKQIRNFLFILFVSLGVPVLNMGDECGQSSGGFTAYDGIKP 707

Query: 682  FNWVTLKTGFGKQTTQFIXXXXXXXXXXXXXXXXXXXLKEENIEWHGADQAPPRWEDPSC 503
            F+W +LKTGFGKQT+QFI                   LKEENIEW+G+D APPRWED SC
Sbjct: 708  FSWSSLKTGFGKQTSQFIFFLSSFRRRRSDLLQRMSFLKEENIEWYGSDGAPPRWEDLSC 767

Query: 502  KFLAMTLKAEEREFQESSSVSSDILGDLFIAFNAADHPETVVLPFPPEGMSWYRLVDTAL 323
            KFLAM LKAEE+EF E SSVSSDI GDLFIAFNAA HPET VLP PPEGM WYRLVDTAL
Sbjct: 768  KFLAMALKAEEKEFLE-SSVSSDISGDLFIAFNAAGHPETAVLPLPPEGMLWYRLVDTAL 826

Query: 322  PFPGFFLTNGELVPEEIPGLSTYQMKPYSCTLFEAKNHT 206
            PFPGFF  +GE+VPE+  GL TY+MK YSCTLFEA N T
Sbjct: 827  PFPGFFSASGEVVPEQTAGLFTYRMKSYSCTLFEANNST 865


>dbj|BAF52942.1| isoamylase-type starch-debranching enzyme 2 [Phaseolus vulgaris]
          Length = 865

 Score = 1155 bits (2988), Expect = 0.0
 Identities = 604/879 (68%), Positives = 677/879 (77%), Gaps = 25/879 (2%)
 Frame = -2

Query: 2767 MANLVPSLSFSVIPWNQQHKHGTKCPFFHNYSFTQRKQQHQLVSRNLQDLTNPFSQNLTT 2588
            MA++V + SFS+IP+NQ HK G KC  FH     QRKQ+       LQ+   PF +N ++
Sbjct: 1    MASVVSAFSFSLIPFNQ-HKFGAKCVGFHK-PLIQRKQRQL----GLQNHFFPFFRNPSS 54

Query: 2587 KLCATSRLSVEETEQKITPFTQSEDLKTALPYLFRTETGDGLXXXXXXXXXXXXXXYIEI 2408
            KLCATSRLS+EETEQ++ PFT+ EDLK AL YLFRTETG GL              YIEI
Sbjct: 55   KLCATSRLSIEETEQQVEPFTRPEDLKGALAYLFRTETGGGLVKVYVTKKKDRYFVYIEI 114

Query: 2407 SSLELSG-GEGEALVLCWGVYRVDSS-------------SVEAMNVTPLVQTSQGKFAVE 2270
            SSL+++  G+ E LVLCWGVYR DSS             + + MNV+PLVQTS  KF VE
Sbjct: 115  SSLDVNHCGDSETLVLCWGVYRGDSSCFVDMDSTGLSGNAAKRMNVSPLVQTSVCKFGVE 174

Query: 2269 LEFDARQVPLYLSFLLRSSL----EIRSHRKRNFCVPVXXXXXXXXXXXXXXXXXXSMNF 2102
            LEFDA+ VPLYLSF L SSL    EI SHR+ NFCVPV                  S+NF
Sbjct: 175  LEFDAKYVPLYLSFFLMSSLDAGLEIISHRRTNFCVPVGLLPGYPGPLGLSYSPDGSVNF 234

Query: 2101 AVFSRHAEGVVLCLYDDDDTSVEEKKPALELDLDPYVNKSGDIWHISFESALNFVSYGYR 1922
            A+FSRHAE VVLCLYD+       +KPALE+DLDPYVN+SGDIWH+SFES  +FVSYGYR
Sbjct: 235  AIFSRHAESVVLCLYDEKGV----EKPALEVDLDPYVNRSGDIWHVSFESVKSFVSYGYR 290

Query: 1921 FLH-TNKDDKG------VVLDPYARIVGNSLPNGVGSVKKNLGCLGKEPAFDWGDDYHPN 1763
                 +K + G      VVLDPYA+IVG+S P+G+  V+ NLG LGKEPAFDWG D+ P+
Sbjct: 291  CRGGVHKQNNGDSSAELVVLDPYAKIVGHSYPSGLELVQ-NLGWLGKEPAFDWGGDFLPD 349

Query: 1762 LAMEELVVYRLNVKRFTEHESSQLSSDLAGTFSGLAKKLQHFKDLGVNAVLLEPVFTFDE 1583
            L+MEELVVYRLNVKRFT+H SSQL S  AGTF+GLA+K+QHFKDLGVNAVLLEPVFT DE
Sbjct: 350  LSMEELVVYRLNVKRFTQHNSSQLPSGSAGTFTGLAEKVQHFKDLGVNAVLLEPVFTSDE 409

Query: 1582 EKGAYFPCHFFSPMNLYGPSGGPVSTVTSMKEMVKTMHANGIEVLIEVVFSNTADTGALQ 1403
            +KG YFPCHFFS M++YGPSGGPVST+ SMKEMVKTMHANGIEVL+EVVFSNTA+ GALQ
Sbjct: 410  KKGPYFPCHFFSLMHIYGPSGGPVSTMASMKEMVKTMHANGIEVLVEVVFSNTAEIGALQ 469

Query: 1402 GIDDLSYYYANGVGDLKVHSALNCNYPIVQNLILDSLRHWVTEFHIDGFSFVNASHLLRG 1223
            GIDD SYY ANGVGDLK+ SALNCNYPIVQNLILDSLR+WVTEFHIDGFSF+NASHLLRG
Sbjct: 470  GIDDSSYYLANGVGDLKIQSALNCNYPIVQNLILDSLRYWVTEFHIDGFSFINASHLLRG 529

Query: 1222 FHGEHLSRPPSVEAIAFDPVLWKTKIIADCWDPHDMVAKETRFPHWMRWAEINTNFCNDV 1043
            FHGE+LSRPP VEAIAFDPVL KTKIIADCWDPHD VAKE  FPHWMRWAE+N  FCNDV
Sbjct: 530  FHGEYLSRPPLVEAIAFDPVLSKTKIIADCWDPHDTVAKEIHFPHWMRWAEMNAKFCNDV 589

Query: 1042 RNFLRGENLLSNLATRLCGSGDMFSDGRGPAFSLNYIARNFGLSLVDIVSFSSDDVAAEL 863
            RNF RGENLLS+LATRLCGSGDMFS GRGPAFS NYI RNFG SLVD+VSFSSDD   EL
Sbjct: 590  RNFFRGENLLSDLATRLCGSGDMFSGGRGPAFSFNYIVRNFGFSLVDLVSFSSDD---EL 646

Query: 862  SWNCGEEGPTNNRTVLERRLKQIRNFLFILFMSLGVPVLNMGDECGQSSDGSPAYGDMKP 683
            SWNCGEEGPTNN +VLERRLKQIRNFLFILF+SLGVPVLNMGDEC  SS G PAY D+KP
Sbjct: 647  SWNCGEEGPTNNTSVLERRLKQIRNFLFILFVSLGVPVLNMGDECAHSSGGFPAYDDIKP 706

Query: 682  FNWVTLKTGFGKQTTQFIXXXXXXXXXXXXXXXXXXXLKEENIEWHGADQAPPRWEDPSC 503
              W  L TGFGKQ +QFI                   LKEENIEW+G+D APPRWEDPSC
Sbjct: 707  MTWSALTTGFGKQISQFIFFMSSLRRRRSDLLQRRSFLKEENIEWYGSDGAPPRWEDPSC 766

Query: 502  KFLAMTLKAEEREFQESSSVSSDILGDLFIAFNAADHPETVVLPFPPEGMSWYRLVDTAL 323
            KFLAMTLK+E     E SSVSSDI GD+FIA N AD PE+ VLP PPEGMSWYRLVDT+L
Sbjct: 767  KFLAMTLKSEVAVLSE-SSVSSDISGDIFIALNVADEPESTVLPLPPEGMSWYRLVDTSL 825

Query: 322  PFPGFFLTNGELVPEEIPGLSTYQMKPYSCTLFEAKNHT 206
            PFPGFF ++GE+VPE   GLSTY+MK +SC LFEA N T
Sbjct: 826  PFPGFFSSSGEIVPEMKAGLSTYKMKSHSCALFEACNPT 864


>ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Vitis vinifera]
          Length = 882

 Score =  969 bits (2504), Expect = 0.0
 Identities = 501/831 (60%), Positives = 601/831 (72%), Gaps = 28/831 (3%)
 Frame = -2

Query: 2626 QDLTNPFSQNLTTKLCATSRLSVEETEQKITPFTQSEDLKTALPYLFRTETGDGLXXXXX 2447
            Q+ T    +N+  K+ ATS +++EETE + +  T+SE+L++ L YLF TE G G      
Sbjct: 57   QNATRNCFRNINWKVSATSHIAIEETENRYST-TESEELESRLNYLFLTEIG-GQVKVIV 114

Query: 2446 XXXXXXXXXYIEISSLELSGGEGEALVLCWGVYRVDSSS---VEAMNVTPLV-------- 2300
                      IE+SSL+L   + + L+L WGV+R +SS    V+  N+ P V        
Sbjct: 115  GKKNKKYIVSIEVSSLQLYNSDNK-LILSWGVFRSNSSCFMPVDFQNLVPEVGSNTTEIP 173

Query: 2299 --QTSQGKFAVELEFDARQVPLYLSFLLRSSLE-------IRSHRKRNFCVPVXXXXXXX 2147
              + S G FA++L+F+A   P YLSFLL+S+L+       IRSHRK NFC+PV       
Sbjct: 174  FMERSSGSFALKLDFEANHAPFYLSFLLKSTLDTDLSSSDIRSHRKTNFCIPVGFGRGYP 233

Query: 2146 XXXXXXXXXXXSMNFAVFSRHAEGVVLCLYDDDDTSVEEKKPALELDLDPYVNKSGDIWH 1967
                       S NFA FSR+A GVVLCLYD         KPALE+DLDPYVN++GDIWH
Sbjct: 234  APLGLSFSSDGSPNFAFFSRNAGGVVLCLYDGTSD-----KPALEIDLDPYVNRTGDIWH 288

Query: 1966 ISFESALNFVSYGYRFLHTNKDDKG-------VVLDPYARIVGNSLPNGVG-SVKKNLGC 1811
             S ES  +FVSYGYR    N  D G       V LDPYA+++ NS  +  G   +  LG 
Sbjct: 289  ASMESVGSFVSYGYRCKEANLQDSGETLHTEHVHLDPYAKLIRNSFSDDHGLKPQPRLGE 348

Query: 1810 LGKEPAFDWGDDYHPNLAMEELVVYRLNVKRFTEHESSQLSSDLAGTFSGLAKKLQHFKD 1631
            L KEPAF+W DD HP + ME+LVVYRLNV  FT+ ESSQ++SDLAGTFSGL +KL HFKD
Sbjct: 349  LQKEPAFNWNDDVHPYIPMEKLVVYRLNVMHFTKDESSQVASDLAGTFSGLMEKLHHFKD 408

Query: 1630 LGVNAVLLEPVFTFDEEKGAYFPCHFFSPMNLYGPSGGPVSTVTSMKEMVKTMHANGIEV 1451
            LGVNAVLLEP+F+FDE+KG YFP HFFSPMN+YGPS GPVST+ S+KEMVK +HANGIEV
Sbjct: 409  LGVNAVLLEPIFSFDEQKGPYFPFHFFSPMNVYGPSSGPVSTINSVKEMVKRLHANGIEV 468

Query: 1450 LIEVVFSNTADTGALQGIDDLSYYYANGVGDLKVHSALNCNYPIVQNLILDSLRHWVTEF 1271
            L+EVVF++TA++GALQGIDD  YYY NG  DL + +ALNCNY IVQ +I+DSLR+WVTEF
Sbjct: 469  LLEVVFTHTAESGALQGIDDSCYYYVNGDADLGIRNALNCNYSIVQQMIVDSLRYWVTEF 528

Query: 1270 HIDGFSFVNASHLLRGFHGEHLSRPPSVEAIAFDPVLWKTKIIADCWDPHDMVAKETRFP 1091
            H+DGF F+NAS LLRGFHGE+LSRPP VE IAFDP+L KTKIIADCWDP +M+ KE RFP
Sbjct: 529  HVDGFCFINASSLLRGFHGEYLSRPPLVETIAFDPLLSKTKIIADCWDPRNMLPKEIRFP 588

Query: 1090 HWMRWAEINTNFCNDVRNFLRGENLLSNLATRLCGSGDMFSDGRGPAFSLNYIARNFGLS 911
            HW RWAE+NT FCNDVRNFLRGE  LS+ ATRLCGSGD+F DGRGPAFS N+  +NFGL 
Sbjct: 589  HWKRWAEVNTRFCNDVRNFLRGEG-LSDFATRLCGSGDIFMDGRGPAFSFNFTTKNFGLP 647

Query: 910  LVDIVSFSSDDVAAELSWNCGEEGPTNNRTVLERRLKQIRNFLFILFMSLGVPVLNMGDE 731
            LVD+VSFSS ++A+ELSWNCG+EGPTN  TVLERRLKQIRNFLFIL++SLGVP+LNMGDE
Sbjct: 648  LVDLVSFSSSELASELSWNCGDEGPTNKTTVLERRLKQIRNFLFILYVSLGVPILNMGDE 707

Query: 730  CGQSSDGSPAYGDMKPFNWVTLKTGFGKQTTQFIXXXXXXXXXXXXXXXXXXXLKEENIE 551
            CGQSS GSPAYGD KPFNW ++KTGFG QT QFI                   LKEE+I+
Sbjct: 708  CGQSSGGSPAYGDRKPFNWNSVKTGFGIQTIQFISFLSSLRSRRSDLLQRRSFLKEESID 767

Query: 550  WHGADQAPPRWEDPSCKFLAMTLKAEEREFQESSSVSSDILGDLFIAFNAADHPETVVLP 371
            WHG+DQ+PPRW+DPS KFLAMTLKAE  E Q  S  SS I GDLFIAFN AD    V+LP
Sbjct: 768  WHGSDQSPPRWDDPSSKFLAMTLKAENMEGQLPSE-SSSIKGDLFIAFNTADRSVKVILP 826

Query: 370  FPPEGMSWYRLVDTALPFPGFFLTNGELVPEEIPGLSTYQMKPYSCTLFEA 218
             PP GM W+RLVDTALPFPGFF  +GE + ++  GL TY+M+ +SC LFEA
Sbjct: 827  PPPTGMVWHRLVDTALPFPGFFTADGEAILKKKSGLVTYKMESHSCALFEA 877