BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Glycyrrhiza23_contig00013295
         (1936 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_003638310.1| Cytochrome P450 monooxygenase [Medicago trun...   653   0.0  
ref|XP_003532671.1| PREDICTED: isoflavone 2'-hydroxylase-like [G...   647   0.0  
gb|AAQ20041.1| isoflavone 3'-hydroxylase, partial [Medicago trun...   569   e-160
gb|AFA89978.1| isoflavone-3'-hydroxylase [Astragalus membranaceus]    567   e-159
ref|XP_003532672.1| PREDICTED: isoflavone 2'-hydroxylase-like [G...   557   e-156

>ref|XP_003638310.1| Cytochrome P450 monooxygenase [Medicago truncatula]
            gi|355504245|gb|AES85448.1| Cytochrome P450 monooxygenase
            [Medicago truncatula]
          Length = 494

 Score =  653 bits (1684), Expect = 0.0
 Identities = 337/504 (66%), Positives = 401/504 (79%)
 Frame = +2

Query: 113  MAPLSYYTVLLSLAFSLAFYKLLLQRYLRTFKNLPPCPVPTLPMIGNLHHLKNPRLHRSL 292
            + PL YY+ LLSL+F +   KLLLQR  R FKNLPP P  +LP+IGNLH++  P LHRSL
Sbjct: 4    ITPLLYYS-LLSLSFIVTL-KLLLQR--RKFKNLPPGPSISLPIIGNLHNINLP-LHRSL 58

Query: 293  HSLSQKHGHIFSLWFGSRLAVVVSSQTLAQRCFTRHDTVLANRPRFLTGKHLFYNYTSLD 472
            H+LSQK+G IF+LWFGS   VV+SSQTL Q+C T+HD  L+NRPRFLTGK+LFYNYTSLD
Sbjct: 59   HNLSQKYGKIFTLWFGSSRVVVISSQTLLQQCLTKHDVDLSNRPRFLTGKYLFYNYTSLD 118

Query: 473  SAPYGDHWRNLRRIITTDVLSTHRLNSFYQTRKHEAVTVVRQLAAMDYTCEGLLFARVEL 652
            SAPYGDHWRNLRRIIT D+LST RLNSF  +R+ E + ++ +LA    T E   F RVEL
Sbjct: 119  SAPYGDHWRNLRRIITLDILSTQRLNSFVGSRRSETLKLIIKLAKD--TSER--FTRVEL 174

Query: 653  RSRLTRMVFNNMMRMVSGDDVAAEEGRRFREVLSEMQPLFGAGNKGEFLPLLRWLGLDVL 832
            R+RLT M F  M +MV+GD   AEEG++F+E+++EM PLFGA N G+F+PLLR    D L
Sbjct: 175  RTRLTDMTFEIMTKMVAGD---AEEGKKFKEMINEMMPLFGASNMGDFVPLLRLFDFDGL 231

Query: 833  VKRMKEIGKRADGILQGIVEKHRSDGEKKLERGNSMIQHLLTLQKSQPEYYSDHIIKGLI 1012
            VKRMK+IGKR D  LQGIVE+ RS         N+M+QHLLTLQKSQP+YYSD IIKGL+
Sbjct: 232  VKRMKDIGKRGDSFLQGIVEEIRSGKHGD----NNMLQHLLTLQKSQPDYYSDEIIKGLV 287

Query: 1013 QGMFLGGTDTTSATIEWAMSALLNHPEMLEKAKNEIDTHIGQDRLVDESDIPNLPYLQNI 1192
            QGMFL GTDTT+ T+EWA++ LLNHP +L+KAK+E++T IG DRLV+ESDIPNL Y+QNI
Sbjct: 288  QGMFLAGTDTTAVTLEWAIATLLNHPNILKKAKDELNTQIGYDRLVEESDIPNLSYIQNI 347

Query: 1193 IHETXXXXXXXXXXXXHFSSGECNIRGYTIPRDTIVMINAWAIQRDPEIWSDAACFKPER 1372
            I+ET             FSS ECNI G+TIPRDTIV+INAWAIQRDPE WSDA+CFKPER
Sbjct: 348  IYETLRLYSPAPLLLPRFSSNECNIEGFTIPRDTIVLINAWAIQRDPETWSDASCFKPER 407

Query: 1373 FEKEGEANKLMAFGLGRRACPGLGLAHRTIGLTLGLLIQCFEWKRLIDHEEIDMTENKSG 1552
            FEKEGEANKL+AFG GRR CPG+GLAHRT+ L+LGLLIQCFEWKRL D EE+DMTENK G
Sbjct: 408  FEKEGEANKLIAFGFGRRGCPGIGLAHRTMALSLGLLIQCFEWKRLND-EEVDMTENKIG 466

Query: 1553 VIMEKLIPLEAMCKSRPIIKKVMQ 1624
            V+ +KL PLEAMCK+RPII KV+Q
Sbjct: 467  VVTQKLNPLEAMCKARPIINKVIQ 490


>ref|XP_003532671.1| PREDICTED: isoflavone 2'-hydroxylase-like [Glycine max]
          Length = 500

 Score =  647 bits (1670), Expect = 0.0
 Identities = 340/516 (65%), Positives = 400/516 (77%), Gaps = 8/516 (1%)
 Frame = +2

Query: 113  MAPLSYYTVLLSLAFSLAFYKLLLQRYLRTFKNLPPCPVPTLPMIGNLHHLKNPRLHRSL 292
            M PL  Y+ LL L   L F    +QR  R  KNLPPCP P+LP+IGNLH++K+P LHRSL
Sbjct: 1    MGPL-LYSSLLVLVLILRF----VQR--RRVKNLPPCP-PSLPLIGNLHYIKSP-LHRSL 51

Query: 293  HSLSQKHGHIFSLWFGSRLAVVVSSQTLAQRCFTRHDTVLANRPRFLTGKHLFYNYTSLD 472
             SLS+K+G IFSLWFGSR  VV+SS TL Q CFT+HD VLANRPRFLTGK+LFYNY+S+ 
Sbjct: 52   LSLSEKYGPIFSLWFGSRFVVVISSPTLLQECFTKHDIVLANRPRFLTGKYLFYNYSSMG 111

Query: 473  SAPYGDHWRNLRRIITTDVLSTHRLNSFYQTRKHEAVTVVRQLAAMDYTCEGLLFARVEL 652
            S+PYGDHWRNLRRIIT DVLST RLNSF++ R+ E + V+++LA    TC G  FA V L
Sbjct: 112  SSPYGDHWRNLRRIITIDVLSTSRLNSFFEIRREETMRVIQKLARE--TCNG--FALVHL 167

Query: 653  RSRLTRMVFNNMMRMVSG-----DDVAA---EEGRRFREVLSEMQPLFGAGNKGEFLPLL 808
            R RLT M FNNMMRM+SG     DD+ A   EE ++FR++++E+  L GA NKG+FLP L
Sbjct: 168  RPRLTEMTFNNMMRMISGKRYYGDDIEAADAEEAKQFRDIMTEVMSLLGANNKGDFLPFL 227

Query: 809  RWLGLDVLVKRMKEIGKRADGILQGIVEKHRSDGEKKLERGNSMIQHLLTLQKSQPEYYS 988
            RW   D L KR+K I  RAD  LQG++E+HRS   K     N+MI+HLLT+Q+SQP YYS
Sbjct: 228  RWFDFDGLEKRLKVISTRADSFLQGLLEEHRSGKHK----ANTMIEHLLTMQESQPHYYS 283

Query: 989  DHIIKGLIQGMFLGGTDTTSATIEWAMSALLNHPEMLEKAKNEIDTHIGQDRLVDESDIP 1168
            DHIIKGLIQGM L GTDTT+  IEWA+S+LLNHPE+L+KAK+EID  +GQDRLVDESDIP
Sbjct: 284  DHIIKGLIQGMLLAGTDTTAVAIEWAVSSLLNHPEILKKAKDEIDNMVGQDRLVDESDIP 343

Query: 1169 NLPYLQNIIHETXXXXXXXXXXXXHFSSGECNIRGYTIPRDTIVMINAWAIQRDPEIWSD 1348
             LPYLQNII+ET            H+SS EC I G+TIPRDTIV+INAWAIQRDPE WSD
Sbjct: 344  KLPYLQNIIYETLRLFAPAPLLLPHYSSEECTIGGFTIPRDTIVLINAWAIQRDPEHWSD 403

Query: 1349 AACFKPERFEKEGEANKLMAFGLGRRACPGLGLAHRTIGLTLGLLIQCFEWKRLIDHEEI 1528
            A CFKPERFE+EGEANKL+ FGLGRRACPG+GLAHR++GLTLGLLIQCFEWKR  D EEI
Sbjct: 404  ATCFKPERFEQEGEANKLIPFGLGRRACPGIGLAHRSMGLTLGLLIQCFEWKRPTD-EEI 462

Query: 1529 DMTENKSGVIMEKLIPLEAMCKSRPIIKKVMQ*LII 1636
            DM ENK G+ + KLIPLEAM K+RPI +KVMQ L I
Sbjct: 463  DMRENK-GLALPKLIPLEAMFKTRPISEKVMQQLAI 497


>gb|AAQ20041.1| isoflavone 3'-hydroxylase, partial [Medicago truncatula]
          Length = 501

 Score =  569 bits (1467), Expect = e-160
 Identities = 296/505 (58%), Positives = 364/505 (72%), Gaps = 8/505 (1%)
 Frame = +2

Query: 128  YYTVLLSLAFSLAFYKLLLQRYLRTFKNLPPCPVPTLPMIGNLHHLKNPRLHRSLHSLSQ 307
            +Y  LLSL+F +   K+LL+   R  KNLPP P PT+P+IGNLHHLK+P LHR+  +LSQ
Sbjct: 3    FYYSLLSLSFIITI-KILLKITSRRLKNLPPGP-PTIPIIGNLHHLKHP-LHRTFTTLSQ 59

Query: 308  KHGHIFSLWFGSRLAVVVSSQTLAQRCFTRHDTVLANRPRFLTGKHLFYNYTSLDSAPYG 487
             +G IFSLWFGSRL VVVSS +LA  CFT++D +LANRPRFLTGK++FYNYT+L SA YG
Sbjct: 60   TYGDIFSLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYG 119

Query: 488  DHWRNLRRIITTDVLSTHRLNSFYQTRKHEAVTVVRQLAAMDYTCEGLLFARVELRSRLT 667
            DHWRNLRRI T DVLS +RLNSF   R+ E   ++++L   D   EG  F +VELR RLT
Sbjct: 120  DHWRNLRRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLK-DVVSEGFGFTKVELRPRLT 178

Query: 668  RMVFNNMMRMVSGD-------DVA-AEEGRRFREVLSEMQPLFGAGNKGEFLPLLRWLGL 823
             M FN MMRM+SG        DV+  EE ++FRE++SEM  L GA NKG+FLPLLR + L
Sbjct: 179  EMTFNAMMRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDL 238

Query: 824  DVLVKRMKEIGKRADGILQGIVEKHRSDGEKKLERGNSMIQHLLTLQKSQPEYYSDHIIK 1003
            D L KR K I KR++  L+G++E+HR         G +MI HLL L +SQPEYYSDH+IK
Sbjct: 239  DNLEKRCKRIAKRSNAFLEGLIEEHRRGNIHS--DGGTMIDHLLKLSESQPEYYSDHLIK 296

Query: 1004 GLIQGMFLGGTDTTSATIEWAMSALLNHPEMLEKAKNEIDTHIGQDRLVDESDIPNLPYL 1183
            GLIQGM L GTDT++ TIEW MS LLNHPE+L+KAK E+DT IG+++LVDE D+  LPYL
Sbjct: 297  GLIQGMLLAGTDTSAVTIEWVMSELLNHPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYL 356

Query: 1184 QNIIHETXXXXXXXXXXXXHFSSGECNIRGYTIPRDTIVMINAWAIQRDPEIWSDAACFK 1363
            QNII ET            H+SS +C I  + +P+DTI++ N W I RDP+ W+DA  FK
Sbjct: 357  QNIISETLRLHPPAPLLLPHYSSEDCTIGEFNVPKDTIILTNVWGIHRDPKHWNDALSFK 416

Query: 1364 PERFEKEGEANKLMAFGLGRRACPGLGLAHRTIGLTLGLLIQCFEWKRLIDHEEIDMTEN 1543
            PERFEKE E NK+MAFGLGRRACPGL LA RT+G T+GLLIQCFEW+R    E++DM E 
Sbjct: 417  PERFEKEEEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWER-ESEEKLDMMEG 475

Query: 1544 KSGVIMEKLIPLEAMCKSRPIIKKV 1618
            K G+ M   IPL AMCK+ PI   V
Sbjct: 476  K-GITMPMKIPLRAMCKALPIANDV 499


>gb|AFA89978.1| isoflavone-3'-hydroxylase [Astragalus membranaceus]
          Length = 505

 Score =  567 bits (1462), Expect = e-159
 Identities = 295/513 (57%), Positives = 371/513 (72%), Gaps = 9/513 (1%)
 Frame = +2

Query: 113  MAPLSYYTVLLSLAFSLAFYKLLLQRYLRTFKNLPPCPVPTLPMIGNLHHLKNPRLHRSL 292
            MAPL YY+ LLSLAF L   K++LQ   R  KNLPP P PT+P+IGNLHHLK+P LHR+ 
Sbjct: 1    MAPLLYYS-LLSLAFILTV-KIILQIQSRRLKNLPPGP-PTIPIIGNLHHLKHP-LHRTF 56

Query: 293  HSLSQKHGHIFSLWFGSRLAVVVSSQTLAQRCFTRHDTVLANRPRFLTGKHLFYNYTSLD 472
             +LSQK+G I SLWFGSRL VVV+S ++AQ CFT++D VLANRP+FLTGK++FYNYT+L 
Sbjct: 57   TTLSQKYGDIISLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLG 116

Query: 473  SAPYGDHWRNLRRIITTDVLSTHRLNSFYQTRKHEAVTVVRQLAAMDYTCEG-LLFARVE 649
            SA YGDHWRNLRRI T DVLS HRLNSF+  RK E + +V +L   D   EG   F  VE
Sbjct: 117  SASYGDHWRNLRRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRN-DVVKEGNFSFTDVE 175

Query: 650  LRSRLTRMVFNNMMRMVSG--------DDVAAEEGRRFREVLSEMQPLFGAGNKGEFLPL 805
            LR RLT M FN MMRM+SG        D    +E ++FR+++SE+  L GA NKG+FLPL
Sbjct: 176  LRHRLTEMTFNAMMRMISGKRYYGDDGDVTDVKEAKQFRDIISEILSLLGANNKGDFLPL 235

Query: 806  LRWLGLDVLVKRMKEIGKRADGILQGIVEKHRSDGEKKLERGNSMIQHLLTLQKSQPEYY 985
            LR   LD L KR K I KRAD  LQG++E+H++      + G++MI HLL L+++QPEYY
Sbjct: 236  LRLFDLDHLEKRCKRISKRADAFLQGLIEQHQNANHSDND-GDTMIDHLLKLRETQPEYY 294

Query: 986  SDHIIKGLIQGMFLGGTDTTSATIEWAMSALLNHPEMLEKAKNEIDTHIGQDRLVDESDI 1165
            SDH+IKGLIQ M L GTDT++ TIEW M+ LLN+PE+L+KAK EI+T IG++RLV+E D+
Sbjct: 295  SDHMIKGLIQAMLLAGTDTSAVTIEWVMAELLNNPEVLKKAKKEIETTIGKERLVEEQDL 354

Query: 1166 PNLPYLQNIIHETXXXXXXXXXXXXHFSSGECNIRGYTIPRDTIVMINAWAIQRDPEIWS 1345
              LPYLQN+I E             H +S +C I G+ +P+DTI++ N WAI RDPE+W+
Sbjct: 355  SQLPYLQNVISEALRLHPPAPLLLPHSASEDCTIGGFNVPKDTIILTNIWAIHRDPELWT 414

Query: 1346 DAACFKPERFEKEGEANKLMAFGLGRRACPGLGLAHRTIGLTLGLLIQCFEWKRLIDHEE 1525
            D + FKPERFEKEGE NKL++FGLGRRACPGL LA RT+G T+GLLIQ FEWK     E+
Sbjct: 415  DPSSFKPERFEKEGEVNKLLSFGLGRRACPGLSLAQRTVGYTVGLLIQGFEWK-TESEEK 473

Query: 1526 IDMTENKSGVIMEKLIPLEAMCKSRPIIKKVMQ 1624
            +D+ E K G+ M    PL AMCK  PI+  +M+
Sbjct: 474  LDLAEGK-GITMPMKFPLRAMCKPLPIVNDIMK 505


>ref|XP_003532672.1| PREDICTED: isoflavone 2'-hydroxylase-like [Glycine max]
          Length = 502

 Score =  557 bits (1435), Expect = e-156
 Identities = 284/510 (55%), Positives = 371/510 (72%), Gaps = 8/510 (1%)
 Frame = +2

Query: 113  MAPLSYYTVLLSLAFSLAFYKLLLQRYLRTFKNLPPCPVPTLPMIGNLHHLKNPRLHRSL 292
            M  LS+ +  L   F     KLLLQ   R F+NLPP P P+LP+IGNLHHLK P LHR+ 
Sbjct: 1    MEILSFLSYSLLYVFLFLLLKLLLQA--RKFQNLPPGP-PSLPIIGNLHHLKRP-LHRTF 56

Query: 293  HSLSQKHGHIFSLWFGSRLAVVVSSQTLAQRCFTRHDTVLANRPRFLTGKHLFYNYTSLD 472
             +LS K+GH+ SLWFGSRL VVVSSQTL Q CFT++D VLANRPRFL+GKH+FYNYT+L 
Sbjct: 57   RALSDKYGHVISLWFGSRLVVVVSSQTLFQECFTKNDVVLANRPRFLSGKHIFYNYTTLG 116

Query: 473  SAPYGDHWRNLRRIITTDVLSTHRLNSFYQTRKHEAVTVVRQLAAMDYTCEGLLFARVEL 652
            S+PYG+HWRNLRRI   DVLSTHRL+SF   R+ E   +VR+LA    +   L FA VEL
Sbjct: 117  SSPYGEHWRNLRRITALDVLSTHRLHSFAAIRRDETHRLVRKLAEAQGSESSLSFAEVEL 176

Query: 653  RSRLTRMVFNNMMRMVSG--------DDVAAEEGRRFREVLSEMQPLFGAGNKGEFLPLL 808
             S+   M FNN+MRM+SG        D    EE ++FR ++SE+  L GA NK +F+P+L
Sbjct: 177  TSKFYDMTFNNIMRMISGKRYYGDDCDMADVEEAKQFRAMVSELLKLAGANNKNDFMPVL 236

Query: 809  RWLGLDVLVKRMKEIGKRADGILQGIVEKHRSDGEKKLERGNSMIQHLLTLQKSQPEYYS 988
            R    + L KR+K+I  + D  L+G++E+ R+    K +R N+M+ HLL+LQ+SQPEYY+
Sbjct: 237  RLFDFENLEKRLKKISNKTDTFLRGLLEEIRA----KKQRANTMLDHLLSLQESQPEYYT 292

Query: 989  DHIIKGLIQGMFLGGTDTTSATIEWAMSALLNHPEMLEKAKNEIDTHIGQDRLVDESDIP 1168
            D IIKGL  GM +  TD+ + T+EWA+S +LNHPE+ ++A++E++TH+GQD L++ESD+ 
Sbjct: 293  DQIIKGLALGMLIAATDSQAVTLEWALSCVLNHPEVFKRARDELETHVGQDHLLEESDLS 352

Query: 1169 NLPYLQNIIHETXXXXXXXXXXXXHFSSGECNIRGYTIPRDTIVMINAWAIQRDPEIWSD 1348
             LPYL+NII+ET            H SS EC I G+ +P DTIV+INAW+I RDP++WS+
Sbjct: 353  KLPYLKNIIYETLRLYTPAPLLLPHSSSEECIIGGFKVPGDTIVLINAWSIHRDPKVWSE 412

Query: 1349 AACFKPERFEKEGEANKLMAFGLGRRACPGLGLAHRTIGLTLGLLIQCFEWKRLIDHEEI 1528
            A  FKPERFEKEGE +KL+AFGLGRRACPG GLA R + L+LGLLIQCFEWKR+ D +EI
Sbjct: 413  ATSFKPERFEKEGELDKLIAFGLGRRACPGEGLAMRALCLSLGLLIQCFEWKRVGD-KEI 471

Query: 1529 DMTENKSGVIMEKLIPLEAMCKSRPIIKKV 1618
            DM E +SG  + +LIPL+AMCK+RP+I ++
Sbjct: 472  DMRE-ESGFTLSRLIPLKAMCKARPVINRL 500