BLASTX nr result
ID: Glycyrrhiza23_contig00012988
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Glycyrrhiza23_contig00012988
(1715 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
sp|Q84KK6.1|I4OMT_GLYEC RecName: Full=Isoflavone 4'-O-methyltran... 701 0.0
sp|Q29U70.1|I4OMT_MEDTR RecName: Full=Isoflavone 4'-O-methyltran... 607 e-171
sp|Q84KK4.1|I4OMT_LOTJA RecName: Full=Isoflavone 4'-O-methyltran... 606 e-171
sp|O24305.1|M3OM1_PEA RecName: Full=6a-hydroxymaackiain methyltr... 600 e-169
pdb|1ZG3|A Chain A, Crystal Structure Of The Isoflavanone 4'-O-M... 598 e-168
>sp|Q84KK6.1|I4OMT_GLYEC RecName: Full=Isoflavone 4'-O-methyltransferase; Short=GeHI4'OMT;
AltName: Full=2,7,4'-trihydroxyisoflavanone
4'-O-methyltransferase; AltName:
Full=S-adenosyl-L-methionine:2,7,
4'-trihydroxyisoflavanone 4'-O-methyltransferase
gi|28804592|dbj|BAC58011.1| S-adenosyl-L-methionine:
2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase
[Glycyrrhiza echinata]
Length = 367
Score = 701 bits (1808), Expect = 0.0
Identities = 351/367 (95%), Positives = 354/367 (96%), Gaps = 1/367 (0%)
Frame = +1
Query: 172 MDFSTNGSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIADVIHNHGKPITLPQLASA 351
M FSTNGSEE ELYHAQIHLYKHVYNFVSSMALKSAMELGIADVIHNHGKPITLP+LASA
Sbjct: 1 MAFSTNGSEEIELYHAQIHLYKHVYNFVSSMALKSAMELGIADVIHNHGKPITLPELASA 60
Query: 352 LKLHPSKVGVLYRFLRLLTHNGFFAKTTVPSQNGKEGEEEE-TAYALTPPSKLLVKGKPT 528
LKLHPSKVG+LYRFLRLLTHNGFFAKTTVPSQNGK+GEEEE TAYALTPPSKLLVKGKPT
Sbjct: 61 LKLHPSKVGILYRFLRLLTHNGFFAKTTVPSQNGKDGEEEEETAYALTPPSKLLVKGKPT 120
Query: 529 CLASIVRGALHPSSLDMWRSSEKWFNEDKELTLFESATGESFWDFLNKDSESGTLSMFQE 708
CLASIVRGALHPSSLDMWRSSEKWF EDKELTLFESATGESFWDFLNKDSESGTLSMFQE
Sbjct: 121 CLASIVRGALHPSSLDMWRSSEKWFKEDKELTLFESATGESFWDFLNKDSESGTLSMFQE 180
Query: 709 AMAADSQMFKLALKECRHVFEGLESLVDXXXXXXXXXKLIHEEFPHLKCTVFDQPQVVGN 888
AMAADSQMFKLALKECRHVFEGLESLVD KLIHEEFPHLKCTVFDQPQVVGN
Sbjct: 181 AMAADSQMFKLALKECRHVFEGLESLVDVGGGTGGVTKLIHEEFPHLKCTVFDQPQVVGN 240
Query: 889 LSGNENLKFVGGDMFKSIPPADAVLLKWVLHDWNDELSLKILKNSKEAISGKGKEGKVII 1068
LSGNENLKFVGGDMFKSIPPADAVLLKWVLHDWNDELSLKILKNSKEAISGKGKEGKVII
Sbjct: 241 LSGNENLKFVGGDMFKSIPPADAVLLKWVLHDWNDELSLKILKNSKEAISGKGKEGKVII 300
Query: 1069 IDISIDEASGDRELTELQLDYDLVMLTMFNGKEREKKEWEKLISDAGFSSYKITPICGFK 1248
IDISIDEASGDRELTELQLDYDLVMLTMFNGKEREKKEWEKLISDAGFSSYKITPICGFK
Sbjct: 301 IDISIDEASGDRELTELQLDYDLVMLTMFNGKEREKKEWEKLISDAGFSSYKITPICGFK 360
Query: 1249 SLIEVFP 1269
SLIEVFP
Sbjct: 361 SLIEVFP 367
>sp|Q29U70.1|I4OMT_MEDTR RecName: Full=Isoflavone 4'-O-methyltransferase; Short=MtHI4'OMT;
AltName: Full=2,7,4'-trihydroxyisoflavanone
4'-O-methyltransferase gi|62871343|gb|AAY18581.1| SAM
dependent isoflavanone
4'-O-methyltransferase/(+)-6a-hydroxymaackiain-3-0-
methyltransferase [Medicago truncatula]
Length = 364
Score = 607 bits (1566), Expect = e-171
Identities = 308/367 (83%), Positives = 327/367 (89%), Gaps = 1/367 (0%)
Frame = +1
Query: 172 MDFSTNGSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIADVIHNHGKPITLPQLASA 351
M FSTNGSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIAD IHNHGKP+TL +LAS+
Sbjct: 1 MAFSTNGSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIADAIHNHGKPMTLSELASS 60
Query: 352 LKLHPSKVGVLYRFLRLLTHNGFFAKTTVPSQNGKEG-EEEETAYALTPPSKLLVKGKPT 528
LKLHPSKV +L+RFLRLLTHNGFFAKT V GKEG EEEE AY+LTPPSKLL+ GKPT
Sbjct: 61 LKLHPSKVNILHRFLRLLTHNGFFAKTIV---KGKEGDEEEEIAYSLTPPSKLLISGKPT 117
Query: 529 CLASIVRGALHPSSLDMWRSSEKWFNEDKELTLFESATGESFWDFLNKDSESGTLSMFQE 708
CL+SIV+GALHPSSLDMW SS+KWFNEDKE TLFE ATGESFWDFLNKDSES TLSMFQ+
Sbjct: 118 CLSSIVKGALHPSSLDMWSSSKKWFNEDKEQTLFECATGESFWDFLNKDSESSTLSMFQD 177
Query: 709 AMAADSQMFKLALKECRHVFEGLESLVDXXXXXXXXXKLIHEEFPHLKCTVFDQPQVVGN 888
AMA+DS+MFKL L+E + VFEGLESLVD KLIHE FPHLKCTVFDQPQVVGN
Sbjct: 178 AMASDSRMFKLVLQENKRVFEGLESLVDVGGGTGGVTKLIHEIFPHLKCTVFDQPQVVGN 237
Query: 889 LSGNENLKFVGGDMFKSIPPADAVLLKWVLHDWNDELSLKILKNSKEAISGKGKEGKVII 1068
L+GNENL FVGGDMFKSIP ADAVLLKWVLHDWNDE SLKILKNSKEAIS KGK+GKVII
Sbjct: 238 LTGNENLNFVGGDMFKSIPSADAVLLKWVLHDWNDEQSLKILKNSKEAISHKGKDGKVII 297
Query: 1069 IDISIDEASGDRELTELQLDYDLVMLTMFNGKEREKKEWEKLISDAGFSSYKITPICGFK 1248
IDISIDE S DR LTELQLDYDLVMLTMF GKER K+EWEKLI DAGFSSYKITPI GFK
Sbjct: 298 IDISIDETSDDRGLTELQLDYDLVMLTMFLGKERTKQEWEKLIYDAGFSSYKITPISGFK 357
Query: 1249 SLIEVFP 1269
SLIEV+P
Sbjct: 358 SLIEVYP 364
>sp|Q84KK4.1|I4OMT_LOTJA RecName: Full=Isoflavone 4'-O-methyltransferase; Short=LjHI4'OMT;
AltName: Full=2,7,4'-trihydroxyisoflavanone
4'-O-methyltransferase; AltName:
Full=S-adenosyl-L-methionine:
2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase
gi|28804596|dbj|BAC58013.1| S-adenosyl-L-methionine:
2,7,4'-trihydroxyisoflavanone 4'-O-methyltransferase
[Lotus japonicus] gi|388490958|gb|AFK33545.1| unknown
[Lotus japonicus]
Length = 365
Score = 606 bits (1563), Expect = e-171
Identities = 308/368 (83%), Positives = 330/368 (89%), Gaps = 2/368 (0%)
Frame = +1
Query: 172 MDFST-NGSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIADVIHNHGKPITLPQLAS 348
MDFS+ NGSE++EL AQIHLYKHVYNFVSSMALKSAMELGIADVIH+HGKPITLP+LA+
Sbjct: 1 MDFSSSNGSEDTELSQAQIHLYKHVYNFVSSMALKSAMELGIADVIHSHGKPITLPELAT 60
Query: 349 ALKLHPSKVGVLYRFLRLLTHNGFFAKTTVPSQNGKEGEEEETAYALTPPSKLLVKGKPT 528
AL L PSK+GVL+RFLRLLTHNGFFAKTTV + EG EEETAY LTPPSKLLVK T
Sbjct: 61 ALNLRPSKIGVLHRFLRLLTHNGFFAKTTV---SRGEGAEEETAYGLTPPSKLLVKSNST 117
Query: 529 CLASIVRGALHPSSLDMWRSSEKWFNED-KELTLFESATGESFWDFLNKDSESGTLSMFQ 705
CLA IV+GALHPSSLDMWRSS+KWF ED +ELTLFESATGESFW+FLNK++ES TLSMFQ
Sbjct: 118 CLAPIVKGALHPSSLDMWRSSKKWFLEDNEELTLFESATGESFWEFLNKETESDTLSMFQ 177
Query: 706 EAMAADSQMFKLALKECRHVFEGLESLVDXXXXXXXXXKLIHEEFPHLKCTVFDQPQVVG 885
EAMAADS MFKLALKEC+HVFEGL SLVD KLI E FPH+KCTVFDQPQVV
Sbjct: 178 EAMAADSHMFKLALKECKHVFEGLGSLVDVAGGRGGVTKLIREAFPHVKCTVFDQPQVVA 237
Query: 886 NLSGNENLKFVGGDMFKSIPPADAVLLKWVLHDWNDELSLKILKNSKEAISGKGKEGKVI 1065
NL+G+ENL FVGGDMFKS+PPADAVLLKWVLHDWNDELSLKILKN KEAISG+GKEGKVI
Sbjct: 238 NLTGDENLNFVGGDMFKSVPPADAVLLKWVLHDWNDELSLKILKNCKEAISGRGKEGKVI 297
Query: 1066 IIDISIDEASGDRELTELQLDYDLVMLTMFNGKEREKKEWEKLISDAGFSSYKITPICGF 1245
IIDISIDE S DRELTEL+LDYDLVMLTMFNGKEREKKEWEKLI DAGFSSYKITPICGF
Sbjct: 298 IIDISIDETSDDRELTELKLDYDLVMLTMFNGKEREKKEWEKLIYDAGFSSYKITPICGF 357
Query: 1246 KSLIEVFP 1269
KSLIEVFP
Sbjct: 358 KSLIEVFP 365
>sp|O24305.1|M3OM1_PEA RecName: Full=6a-hydroxymaackiain methyltransferase 1; AltName:
Full=Isoflavone 4'-O-methyltransferase
gi|1568637|gb|AAC49856.1| 6a-hydroxymaackiain
methyltransferase [Pisum sativum]
Length = 360
Score = 600 bits (1547), Expect = e-169
Identities = 300/366 (81%), Positives = 326/366 (89%)
Frame = +1
Query: 172 MDFSTNGSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIADVIHNHGKPITLPQLASA 351
MDFSTNGSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIAD IHNHGKP+TLP+L+S+
Sbjct: 1 MDFSTNGSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIADAIHNHGKPMTLPELSSS 60
Query: 352 LKLHPSKVGVLYRFLRLLTHNGFFAKTTVPSQNGKEGEEEETAYALTPPSKLLVKGKPTC 531
LKLHPSKV +LYRFLRLLTHNGFFAKTTV S G EEETAY LTP SKLLV GK TC
Sbjct: 61 LKLHPSKVNILYRFLRLLTHNGFFAKTTVKSNEG----EEETAYVLTPSSKLLVSGKSTC 116
Query: 532 LASIVRGALHPSSLDMWRSSEKWFNEDKELTLFESATGESFWDFLNKDSESGTLSMFQEA 711
L+S+V+GALHPSSLDMW S+KWF+EDKE TLFE ATGE++WDFLNKDS+S LSMFQ+A
Sbjct: 117 LSSLVKGALHPSSLDMWGVSKKWFHEDKEQTLFECATGENYWDFLNKDSDS--LSMFQDA 174
Query: 712 MAADSQMFKLALKECRHVFEGLESLVDXXXXXXXXXKLIHEEFPHLKCTVFDQPQVVGNL 891
MAADS++FKLA++E +HVFEGLESLVD KLIHE FPH+KCTVFDQPQVVGNL
Sbjct: 175 MAADSRLFKLAIQENKHVFEGLESLVDVAGGTGGVAKLIHEAFPHIKCTVFDQPQVVGNL 234
Query: 892 SGNENLKFVGGDMFKSIPPADAVLLKWVLHDWNDELSLKILKNSKEAISGKGKEGKVIII 1071
+GNENL FVGGDMFKS+P ADAVLLKWVLHDWNDELSLKILKNSKEAIS KGK+GKVIII
Sbjct: 235 TGNENLNFVGGDMFKSVPSADAVLLKWVLHDWNDELSLKILKNSKEAISHKGKDGKVIII 294
Query: 1072 DISIDEASGDRELTELQLDYDLVMLTMFNGKEREKKEWEKLISDAGFSSYKITPICGFKS 1251
DISIDE S DR LTELQL+YD+VMLTMF GKER KKEWEKLI DAGFS YKITPICGFKS
Sbjct: 295 DISIDENSDDRGLTELQLEYDVVMLTMFLGKERTKKEWEKLIYDAGFSRYKITPICGFKS 354
Query: 1252 LIEVFP 1269
LIEV+P
Sbjct: 355 LIEVYP 360
>pdb|1ZG3|A Chain A, Crystal Structure Of The Isoflavanone 4'-O-Methyltransferase
Complexed With Sah And 2,7,4'-Trihydroxyisoflavanone
Length = 358
Score = 598 bits (1542), Expect = e-168
Identities = 303/361 (83%), Positives = 322/361 (89%), Gaps = 1/361 (0%)
Frame = +1
Query: 190 GSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIADVIHNHGKPITLPQLASALKLHPS 369
GSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIAD IHNHGKP+TL +LAS+LKLHPS
Sbjct: 1 GSEESELYHAQIHLYKHVYNFVSSMALKSAMELGIADAIHNHGKPMTLSELASSLKLHPS 60
Query: 370 KVGVLYRFLRLLTHNGFFAKTTVPSQNGKEG-EEEETAYALTPPSKLLVKGKPTCLASIV 546
KV +L+RFLRLLTHNGFFAKT V GKEG EEEE AY+LTPPSKLL+ GKPTCL+SIV
Sbjct: 61 KVNILHRFLRLLTHNGFFAKTIV---KGKEGDEEEEIAYSLTPPSKLLISGKPTCLSSIV 117
Query: 547 RGALHPSSLDMWRSSEKWFNEDKELTLFESATGESFWDFLNKDSESGTLSMFQEAMAADS 726
+GALHPSSLDMW SS+KWFNEDKE TLFE ATGESFWDFLNKDSES TLSMFQ+AMA+DS
Sbjct: 118 KGALHPSSLDMWSSSKKWFNEDKEQTLFECATGESFWDFLNKDSESSTLSMFQDAMASDS 177
Query: 727 QMFKLALKECRHVFEGLESLVDXXXXXXXXXKLIHEEFPHLKCTVFDQPQVVGNLSGNEN 906
+MFKL L+E + VFEGLESLVD KLIHE FPHLKCTVFDQPQVVGNL+GNEN
Sbjct: 178 RMFKLVLQENKRVFEGLESLVDVGGGTGGVTKLIHEIFPHLKCTVFDQPQVVGNLTGNEN 237
Query: 907 LKFVGGDMFKSIPPADAVLLKWVLHDWNDELSLKILKNSKEAISGKGKEGKVIIIDISID 1086
L FVGGDMFKSIP ADAVLLKWVLHDWNDE SLKILKNSKEAIS KGK+GKVIIIDISID
Sbjct: 238 LNFVGGDMFKSIPSADAVLLKWVLHDWNDEQSLKILKNSKEAISHKGKDGKVIIIDISID 297
Query: 1087 EASGDRELTELQLDYDLVMLTMFNGKEREKKEWEKLISDAGFSSYKITPICGFKSLIEVF 1266
E S DR LTELQLDYDLVMLTMF GKER K+EWEKLI DAGFSSYKITPI GFKSLIEV+
Sbjct: 298 ETSDDRGLTELQLDYDLVMLTMFLGKERTKQEWEKLIYDAGFSSYKITPISGFKSLIEVY 357
Query: 1267 P 1269
P
Sbjct: 358 P 358