BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Glycyrrhiza23_contig00006042
         (1438 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AFA89978.1| isoflavone-3'-hydroxylase [Astragalus membranaceus]    466   e-159
gb|AAQ20041.1| isoflavone 3'-hydroxylase, partial [Medicago trun...   454   e-159
ref|XP_003548043.1| PREDICTED: isoflavone 2'-hydroxylase-like [G...   417   e-140
gb|AAO31712.1| cytochrome P450 [Pisum sativum]                        415   e-139
ref|XP_003532671.1| PREDICTED: isoflavone 2'-hydroxylase-like [G...   402   e-137

>gb|AFA89978.1| isoflavone-3'-hydroxylase [Astragalus membranaceus]
          Length = 505

 Score =  466 bits (1200), Expect(2) = e-159
 Identities = 248/389 (63%), Positives = 270/389 (69%), Gaps = 1/389 (0%)
 Frame = -1

Query: 1420 SLWFGXXXXXXXXXXXXVQECFNRNDVVLANRPKFLTGKYVFYNYTTLGSAPYGDHWRNL 1241
            SLWFG             QECF +NDVVLANRPKFLTGKY+FYNYTTLGSA YGDHWRNL
Sbjct: 68   SLWFGSRLVVVVASPSIAQECFTKNDVVLANRPKFLTGKYIFYNYTTLGSASYGDHWRNL 127

Query: 1240 RRITTIDVLSNHRLNSFVGIRSDETMRFVQKLRNDAVSANNGS-AKVELRARLTEMTFNE 1064
            RRITTIDVLSNHRLNSF G+R DET+R V+KLRND V   N S   VELR RLTEMTFN 
Sbjct: 128  RRITTIDVLSNHRLNSFHGVRKDETLRLVEKLRNDVVKEGNFSFTDVELRHRLTEMTFNA 187

Query: 1063 MMMMISGKRYYGNDGDVTDVEEARQFREIITEILSLLGANNKGXXXXXXXXXXXXXLVKR 884
            MM MISGKRYYG+DGDVTDV+EA+QFR+II+EILSLLGANNKG             L KR
Sbjct: 188  MMRMISGKRYYGDDGDVTDVKEAKQFRDIISEILSLLGANNKGDFLPLLRLFDLDHLEKR 247

Query: 883  LKRIGKRSDAFLQGLIEQQRGGGDRDTXLLRLFDFDNLVKRLKRIGKRSDAFLQGLIEQQ 704
             KRI KR+DAFLQGLIEQ +     D                                  
Sbjct: 248  CKRISKRADAFLQGLIEQHQNANHSD---------------------------------- 273

Query: 703  RGGKHGGGDRDTMIDHLLKLKESQPEYYSDQTIKGLILAMLLAGTDTSAVTIEWAMSELL 524
                    D DTMIDHLLKL+E+QPEYYSD  IKGLI AMLLAGTDTSAVTIEW M+ELL
Sbjct: 274  -------NDGDTMIDHLLKLRETQPEYYSDHMIKGLIQAMLLAGTDTSAVTIEWVMAELL 326

Query: 523  NNQEVLKKAKDEIDTQVGKERLVEEQDLPKLPYLQNIISETXXXXXXXXXXXXXXPSEDC 344
            NN EVLKKAK EI+T +GKERLVEEQDL +LPYLQN+ISE                SEDC
Sbjct: 327  NNPEVLKKAKKEIETTIGKERLVEEQDLSQLPYLQNVISEALRLHPPAPLLLPHSASEDC 386

Query: 343  TIGGFNVPRDTIILTNVWAIHRDPNLWSD 257
            TIGGFNVP+DTIILTN+WAIHRDP LW+D
Sbjct: 387  TIGGFNVPKDTIILTNIWAIHRDPELWTD 415



 Score =  124 bits (312), Expect(2) = e-159
 Identities = 58/72 (80%), Positives = 66/72 (91%)
 Frame = -2

Query: 216 FKPERFEKEGEVNKLIAFGLGRRACPGLNLAQRTVGFTLGLLIQCFEWERESEEKLDMME 37
           FKPERFEKEGEVNKL++FGLGRRACPGL+LAQRTVG+T+GLLIQ FEW+ ESEEKLD+ E
Sbjct: 419 FKPERFEKEGEVNKLLSFGLGRRACPGLSLAQRTVGYTVGLLIQGFEWKTESEEKLDLAE 478

Query: 36  DKGITMPKRIPL 1
            KGITMP + PL
Sbjct: 479 GKGITMPMKFPL 490


>gb|AAQ20041.1| isoflavone 3'-hydroxylase, partial [Medicago truncatula]
          Length = 501

 Score =  454 bits (1167), Expect(2) = e-159
 Identities = 241/389 (61%), Positives = 267/389 (68%)
 Frame = -1

Query: 1420 SLWFGXXXXXXXXXXXXVQECFNRNDVVLANRPKFLTGKYVFYNYTTLGSAPYGDHWRNL 1241
            SLWFG              ECF +ND++LANRP+FLTGKY+FYNYTTLGSA YGDHWRNL
Sbjct: 66   SLWFGSRLVVVVSSPSLAHECFTKNDIILANRPRFLTGKYIFYNYTTLGSASYGDHWRNL 125

Query: 1240 RRITTIDVLSNHRLNSFVGIRSDETMRFVQKLRNDAVSANNGSAKVELRARLTEMTFNEM 1061
            RRITTIDVLSN+RLNSF+G+R DET R +QKL  D VS   G  KVELR RLTEMTFN M
Sbjct: 126  RRITTIDVLSNNRLNSFLGVRRDETNRLIQKLLKDVVSEGFGFTKVELRPRLTEMTFNAM 185

Query: 1060 MMMISGKRYYGNDGDVTDVEEARQFREIITEILSLLGANNKGXXXXXXXXXXXXXLVKRL 881
            M MISGKRYYG+DGDV+DVEEA+QFREII+E++SLLGANNKG             L KR 
Sbjct: 186  MRMISGKRYYGDDGDVSDVEEAKQFREIISEMMSLLGANNKGDFLPLLRVVDLDNLEKRC 245

Query: 880  KRIGKRSDAFLQGLIEQQRGGGDRDTXLLRLFDFDNLVKRLKRIGKRSDAFLQGLIEQQR 701
            KRI KRS+AFL+GLIE+ R                                        R
Sbjct: 246  KRIAKRSNAFLEGLIEEHR----------------------------------------R 265

Query: 700  GGKHGGGDRDTMIDHLLKLKESQPEYYSDQTIKGLILAMLLAGTDTSAVTIEWAMSELLN 521
            G  H  G   TMIDHLLKL ESQPEYYSD  IKGLI  MLLAGTDTSAVTIEW MSELLN
Sbjct: 266  GNIHSDG--GTMIDHLLKLSESQPEYYSDHLIKGLIQGMLLAGTDTSAVTIEWVMSELLN 323

Query: 520  NQEVLKKAKDEIDTQVGKERLVEEQDLPKLPYLQNIISETXXXXXXXXXXXXXXPSEDCT 341
            + EVLKKAK+E+DTQ+GK +LV+EQDL KLPYLQNIISET               SEDCT
Sbjct: 324  HPEVLKKAKEELDTQIGKNKLVDEQDLSKLPYLQNIISETLRLHPPAPLLLPHYSSEDCT 383

Query: 340  IGGFNVPRDTIILTNVWAIHRDPNLWSDA 254
            IG FNVP+DTIILTNVW IHRDP  W+DA
Sbjct: 384  IGEFNVPKDTIILTNVWGIHRDPKHWNDA 412



 Score =  135 bits (341), Expect(2) = e-159
 Identities = 64/72 (88%), Positives = 69/72 (95%)
 Frame = -2

Query: 216 FKPERFEKEGEVNKLIAFGLGRRACPGLNLAQRTVGFTLGLLIQCFEWERESEEKLDMME 37
           FKPERFEKE EVNK++AFGLGRRACPGL+LAQRTVGFT+GLLIQCFEWERESEEKLDMME
Sbjct: 415 FKPERFEKEEEVNKVMAFGLGRRACPGLSLAQRTVGFTVGLLIQCFEWERESEEKLDMME 474

Query: 36  DKGITMPKRIPL 1
            KGITMP +IPL
Sbjct: 475 GKGITMPMKIPL 486


>ref|XP_003548043.1| PREDICTED: isoflavone 2'-hydroxylase-like [Glycine max]
          Length = 498

 Score =  417 bits (1072), Expect(2) = e-140
 Identities = 220/388 (56%), Positives = 263/388 (67%)
 Frame = -1

Query: 1420 SLWFGXXXXXXXXXXXXVQECFNRNDVVLANRPKFLTGKYVFYNYTTLGSAPYGDHWRNL 1241
            SLWFG            VQECF +ND+VLANRP FLTGKY+ YN TT+  +PYGDHWRNL
Sbjct: 65   SLWFGSRFVVVVSSPLAVQECFTKNDIVLANRPHFLTGKYIGYNNTTVAVSPYGDHWRNL 124

Query: 1240 RRITTIDVLSNHRLNSFVGIRSDETMRFVQKLRNDAVSANNGSAKVELRARLTEMTFNEM 1061
            RRI  ++VLS HR+NSF+  R DE MR VQKL  D+    NG  KVEL++R +EMTFN +
Sbjct: 125  RRIMALEVLSTHRINSFLENRRDEIMRLVQKLARDS---RNGFTKVELKSRFSEMTFNTI 181

Query: 1060 MMMISGKRYYGNDGDVTDVEEARQFREIITEILSLLGANNKGXXXXXXXXXXXXXLVKRL 881
            M M+SGKRYYG D DV+DV+EARQFREII E+++L GANN G                  
Sbjct: 182  MRMVSGKRYYGEDCDVSDVQEARQFREIIKELVTLGGANNPGD----------------- 224

Query: 880  KRIGKRSDAFLQGLIEQQRGGGDRDTXLLRLFDFDNLVKRLKRIGKRSDAFLQGLIEQQR 701
                     FL                LLR FDFD L KRLKRI KR+DAFLQGLI+Q R
Sbjct: 225  ---------FLA---------------LLRWFDFDGLEKRLKRISKRTDAFLQGLIDQHR 260

Query: 700  GGKHGGGDRDTMIDHLLKLKESQPEYYSDQTIKGLILAMLLAGTDTSAVTIEWAMSELLN 521
             GKH     +TMIDHLL  ++SQPEYY+DQ IKGL L MLLAGTDTSAVT+EWAMS LLN
Sbjct: 261  NGKHRA---NTMIDHLLAQQQSQPEYYTDQIIKGLALVMLLAGTDTSAVTLEWAMSNLLN 317

Query: 520  NQEVLKKAKDEIDTQVGKERLVEEQDLPKLPYLQNIISETXXXXXXXXXXXXXXPSEDCT 341
            + E+LKKAK+E+DT +G++RLV+E D+PKLPYLQ+I+ ET               SEDCT
Sbjct: 318  HPEILKKAKNELDTHIGQDRLVDEPDIPKLPYLQSIVYETLRLHPAAPMLVPHLSSEDCT 377

Query: 340  IGGFNVPRDTIILTNVWAIHRDPNLWSD 257
            IG +N+P++TI+L N WAIHRDP LWSD
Sbjct: 378  IGEYNIPQNTILLVNAWAIHRDPKLWSD 405



 Score =  108 bits (269), Expect(2) = e-140
 Identities = 48/72 (66%), Positives = 59/72 (81%)
 Frame = -2

Query: 216 FKPERFEKEGEVNKLIAFGLGRRACPGLNLAQRTVGFTLGLLIQCFEWERESEEKLDMME 37
           FKPERFE E E NKL+ FGLGRRACPG NLAQRT+  TL LLIQCFEW+R +++++DM E
Sbjct: 409 FKPERFENESEANKLLPFGLGRRACPGANLAQRTLSLTLALLIQCFEWKRTTKKEIDMTE 468

Query: 36  DKGITMPKRIPL 1
            KG+T+ K+ PL
Sbjct: 469 GKGLTVSKKYPL 480


>gb|AAO31712.1| cytochrome P450 [Pisum sativum]
          Length = 502

 Score =  415 bits (1067), Expect(2) = e-139
 Identities = 226/389 (58%), Positives = 257/389 (66%)
 Frame = -1

Query: 1420 SLWFGXXXXXXXXXXXXVQECFNRNDVVLANRPKFLTGKYVFYNYTTLGSAPYGDHWRNL 1241
            SLWFG            V+ECF +NDVVLANRP+FLTGKY+FY+YTTLGS  YGDHWRNL
Sbjct: 68   SLWFGSRLVVVISSASLVEECFIKNDVVLANRPRFLTGKYIFYDYTTLGSVSYGDHWRNL 127

Query: 1240 RRITTIDVLSNHRLNSFVGIRSDETMRFVQKLRNDAVSANNGSAKVELRARLTEMTFNEM 1061
            RRITTIDVLSN+RL SF+G+R+DE  R +Q +      A+    KVELR+ LTEMTFN M
Sbjct: 128  RRITTIDVLSNNRLTSFLGVRTDEANRLIQNIIKH--GASRDFIKVELRSILTEMTFNGM 185

Query: 1060 MMMISGKRYYGNDGDVTDVEEARQFREIITEILSLLGANNKGXXXXXXXXXXXXXLVKRL 881
            M MISGKRYYG+DGDVT+VEEA+QFREII+EILSLLGANNKG             L KR 
Sbjct: 186  MRMISGKRYYGDDGDVTEVEEAKQFREIISEILSLLGANNKGDFLPLLRWFDLDDLEKRC 245

Query: 880  KRIGKRSDAFLQGLIEQQRGGGDRDTXLLRLFDFDNLVKRLKRIGKRSDAFLQGLIEQQR 701
            K+I  R+DAFL+GLI+                                         + R
Sbjct: 246  KKIANRADAFLEGLIQ-----------------------------------------EHR 264

Query: 700  GGKHGGGDRDTMIDHLLKLKESQPEYYSDQTIKGLILAMLLAGTDTSAVTIEWAMSELLN 521
               HG G  DTMIDHLLKL E Q        IKGLI AMLLAGTDTSA+ IEW MSELLN
Sbjct: 265  SANHGNG--DTMIDHLLKLSEVQLNIIPSHIIKGLIQAMLLAGTDTSALAIEWVMSELLN 322

Query: 520  NQEVLKKAKDEIDTQVGKERLVEEQDLPKLPYLQNIISETXXXXXXXXXXXXXXPSEDCT 341
            + EVL KAK+EI+TQVGK ++++EQDLPKLPYLQNIISE                SEDCT
Sbjct: 323  HPEVLNKAKEEIETQVGKNKIIDEQDLPKLPYLQNIISEALKLHPPAPLLLPHYSSEDCT 382

Query: 340  IGGFNVPRDTIILTNVWAIHRDPNLWSDA 254
            IGGFNVP+DTIILTNVWAIHRDP  WSDA
Sbjct: 383  IGGFNVPKDTIILTNVWAIHRDPTRWSDA 411



 Score =  109 bits (273), Expect(2) = e-139
 Identities = 54/72 (75%), Positives = 59/72 (81%), Gaps = 1/72 (1%)
 Frame = -2

Query: 216 FKPERFEKEGEVNKLIAFGLGRRACPGLNLAQRTVGFTLGLL-IQCFEWERESEEKLDMM 40
           FKPERFEKEGEVNKL+AFG+GRRACPGL+LAQRTVG   G    QCFEW+RESEEKLDMM
Sbjct: 414 FKPERFEKEGEVNKLMAFGIGRRACPGLSLAQRTVGLNCGACWFQCFEWKRESEEKLDMM 473

Query: 39  EDKGITMPKRIP 4
           E K +   KR P
Sbjct: 474 EGKVLPCLKRSP 485


>ref|XP_003532671.1| PREDICTED: isoflavone 2'-hydroxylase-like [Glycine max]
          Length = 500

 Score =  402 bits (1032), Expect(2) = e-137
 Identities = 214/389 (55%), Positives = 253/389 (65%)
 Frame = -1

Query: 1420 SLWFGXXXXXXXXXXXXVQECFNRNDVVLANRPKFLTGKYVFYNYTTLGSAPYGDHWRNL 1241
            SLWFG            +QECF ++D+VLANRP+FLTGKY+FYNY+++GS+PYGDHWRNL
Sbjct: 63   SLWFGSRFVVVISSPTLLQECFTKHDIVLANRPRFLTGKYLFYNYSSMGSSPYGDHWRNL 122

Query: 1240 RRITTIDVLSNHRLNSFVGIRSDETMRFVQKLRNDAVSANNGSAKVELRARLTEMTFNEM 1061
            RRI TIDVLS  RLNSF  IR +ETMR +QKL  +     NG A V LR RLTEMTFN M
Sbjct: 123  RRIITIDVLSTSRLNSFFEIRREETMRVIQKLARETC---NGFALVHLRPRLTEMTFNNM 179

Query: 1060 MMMISGKRYYGNDGDVTDVEEARQFREIITEILSLLGANNKGXXXXXXXXXXXXXLVKRL 881
            M MISGKRYYG+D +  D EEA+QFR+I+TE++SLLGANNKG             L KRL
Sbjct: 180  MRMISGKRYYGDDIEAADAEEAKQFRDIMTEVMSLLGANNKGDFLPFLRWFDFDGLEKRL 239

Query: 880  KRIGKRSDAFLQGLIEQQRGGGDRDTXLLRLFDFDNLVKRLKRIGKRSDAFLQGLIEQQR 701
            K I  R+D+FLQGL+E+ R                                         
Sbjct: 240  KVISTRADSFLQGLLEEHR----------------------------------------- 258

Query: 700  GGKHGGGDRDTMIDHLLKLKESQPEYYSDQTIKGLILAMLLAGTDTSAVTIEWAMSELLN 521
             GKH     +TMI+HLL ++ESQP YYSD  IKGLI  MLLAGTDT+AV IEWA+S LLN
Sbjct: 259  SGKH---KANTMIEHLLTMQESQPHYYSDHIIKGLIQGMLLAGTDTTAVAIEWAVSSLLN 315

Query: 520  NQEVLKKAKDEIDTQVGKERLVEEQDLPKLPYLQNIISETXXXXXXXXXXXXXXPSEDCT 341
            + E+LKKAKDEID  VG++RLV+E D+PKLPYLQNII ET               SE+CT
Sbjct: 316  HPEILKKAKDEIDNMVGQDRLVDESDIPKLPYLQNIIYETLRLFAPAPLLLPHYSSEECT 375

Query: 340  IGGFNVPRDTIILTNVWAIHRDPNLWSDA 254
            IGGF +PRDTI+L N WAI RDP  WSDA
Sbjct: 376  IGGFTIPRDTIVLINAWAIQRDPEHWSDA 404



 Score =  116 bits (290), Expect(2) = e-137
 Identities = 51/72 (70%), Positives = 63/72 (87%)
 Frame = -2

Query: 216 FKPERFEKEGEVNKLIAFGLGRRACPGLNLAQRTVGFTLGLLIQCFEWERESEEKLDMME 37
           FKPERFE+EGE NKLI FGLGRRACPG+ LA R++G TLGLLIQCFEW+R ++E++DM E
Sbjct: 407 FKPERFEQEGEANKLIPFGLGRRACPGIGLAHRSMGLTLGLLIQCFEWKRPTDEEIDMRE 466

Query: 36  DKGITMPKRIPL 1
           +KG+ +PK IPL
Sbjct: 467 NKGLALPKLIPL 478