BLASTX nr result
ID: Glycyrrhiza23_contig00003715
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Glycyrrhiza23_contig00003715
(3105 letters)
Database: ./nr
23,641,837 sequences; 8,123,359,852 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|NP_001236036.1| with no lysine kinase 12 [Glycine max] gi|22... 979 0.0
ref|XP_003616019.1| With no lysine kinase [Medicago truncatula] ... 963 0.0
ref|NP_001236096.1| with no lysine kinase 13 [Glycine max] gi|22... 825 0.0
ref|XP_003553219.1| PREDICTED: probable serine/threonine-protein... 825 0.0
ref|XP_002319412.1| predicted protein [Populus trichocarpa] gi|2... 772 0.0
>ref|NP_001236036.1| with no lysine kinase 12 [Glycine max] gi|225348653|gb|ACN87288.1|
with no lysine kinase [Glycine max]
Length = 595
Score = 979 bits (2530), Expect = 0.0
Identities = 489/586 (83%), Positives = 522/586 (89%), Gaps = 5/586 (0%)
Frame = +2
Query: 770 DNCYVETDPTGRYGRFGDVLGKGAMKTVYKAIDEFLGIEVAWNQVRLNEVLRNPDDLQRL 949
+NCYVETDPTGRYGRFGDVLGKGAMKTVYKAIDE LGIEVAWNQVRLNE LR PDDLQRL
Sbjct: 3 ENCYVETDPTGRYGRFGDVLGKGAMKTVYKAIDEVLGIEVAWNQVRLNEALRTPDDLQRL 62
Query: 950 YSEVHLLSTLKHQSIIRFYTSWIDVDNKAFNFVTEMFTSGSLREYRKKYKRVSIEAIKSW 1129
YSEVHLLSTLKHQSIIRFYTSWID+DN+AFNF+TE+FTSGSLREYRK YKRV+I+AIK+W
Sbjct: 63 YSEVHLLSTLKHQSIIRFYTSWIDIDNRAFNFITELFTSGSLREYRKNYKRVNIQAIKNW 122
Query: 1130 ARQILQGLVFLHGHDPPVIHRDLKCDNIFVNGHLGQVKIGDLGLAAILRGSQSAHSVIGT 1309
A QILQGLV+LH HDPPVIHRDLKCDNIFVNGHLGQVKIGDLGLAAILRGSQ AHSVIGT
Sbjct: 123 ACQILQGLVYLHCHDPPVIHRDLKCDNIFVNGHLGQVKIGDLGLAAILRGSQLAHSVIGT 182
Query: 1310 PEFMAPELYEEEYNELADVYSFGMCVLEMLTSEYPYSECSNPAQIYKKVTSGKLPLAFFR 1489
PEFMAPELYEEEYNELADVYSFGMCVLEMLTSEYPYSECSNPAQIYKKVTSGKLP+AFFR
Sbjct: 183 PEFMAPELYEEEYNELADVYSFGMCVLEMLTSEYPYSECSNPAQIYKKVTSGKLPMAFFR 242
Query: 1490 VEDVEAQKFIGKCLEPATKRPSAKDLLLDPFLVSDDSSSTMKFAIQKPFLNDSEMEKLQL 1669
+ED+EAQ+FIG+CL PA KRPSAK+LLLDPFLVSDD SST KFAIQKPFLN +EMEKLQL
Sbjct: 243 IEDMEAQRFIGRCLVPAEKRPSAKELLLDPFLVSDDPSSTKKFAIQKPFLNVNEMEKLQL 302
Query: 1670 GDDFTRTEMKVIGKLNPEDETIFLKVQISDNNGSVRHVYFPFDIRTDTPIDVAMEMVKEL 1849
DD RT MKVIGKLNPED+TIFLKVQISD +GS R+V+FPFDI +DTPIDVA EMVKEL
Sbjct: 303 SDDLPRTGMKVIGKLNPEDDTIFLKVQISDKDGSARNVFFPFDILSDTPIDVATEMVKEL 362
Query: 1850 EIADWDPFEIANMIDREISALLPHRWQNKCLDTFHTFSYKDD--DGDEPHHHFRXXXXXX 2023
EIADW+PFEIANMIDREISALLPHR Q+ C D FHTF+Y DD D DEPHHHFR
Sbjct: 363 EIADWEPFEIANMIDREISALLPHRRQSSCSDAFHTFNYLDDDCDDDEPHHHFRSFSSSS 422
Query: 2024 XXXXXIPDLVSKAEEISNRYYWLHDDLHDDNSSRCSSQGTYSNLNYYSVDDH--EYNVAS 2197
+ DLVSKAEEIS+ YYWLHDDLHDD SSRCSSQGTYSNLNYYS+DDH EYNV S
Sbjct: 423 SFQESMSDLVSKAEEISSGYYWLHDDLHDDTSSRCSSQGTYSNLNYYSLDDHHQEYNVPS 482
Query: 2198 IRKDKLPIIKSH-KGTRFSPGEDLSNCNHCKTLVGSQTPSTSKSKMMMIDNRRLTRNRSL 2374
+RKDKLPI KSH KG + S GEDLSN N K +VGSQ P TSKSKMMM ++ RLTRNRSL
Sbjct: 483 LRKDKLPITKSHNKGKKVSSGEDLSNFNQYKLMVGSQVPLTSKSKMMMNNHHRLTRNRSL 542
Query: 2375 IDIRSQLLHRSLVEEVNKRRQFNTVGAVENIGFQSPYDASTK*SGH 2512
IDIRSQLLHRSLVEEVNKRR F TVGAVENIGFQ+P D STK S H
Sbjct: 543 IDIRSQLLHRSLVEEVNKRRLFKTVGAVENIGFQAPCDVSTKRSQH 588
>ref|XP_003616019.1| With no lysine kinase [Medicago truncatula]
gi|355517354|gb|AES98977.1| With no lysine kinase
[Medicago truncatula]
Length = 575
Score = 963 bits (2490), Expect = 0.0
Identities = 475/586 (81%), Positives = 521/586 (88%)
Frame = +2
Query: 731 MFSMRENKVDCKEDNCYVETDPTGRYGRFGDVLGKGAMKTVYKAIDEFLGIEVAWNQVRL 910
MF RE+ + KE+N YVETDPTGRYGRFGDVLGKGAMKTVYKAIDE LGIEVAWNQVRL
Sbjct: 1 MFCKRESMEEFKEENRYVETDPTGRYGRFGDVLGKGAMKTVYKAIDEVLGIEVAWNQVRL 60
Query: 911 NEVLRNPDDLQRLYSEVHLLSTLKHQSIIRFYTSWIDVDNKAFNFVTEMFTSGSLREYRK 1090
NEVL PDDLQRLYSEVHLLSTLKH+SI+RFYTSWID+DNK FNFVTEMFTSGSLREYR+
Sbjct: 61 NEVLNTPDDLQRLYSEVHLLSTLKHRSIMRFYTSWIDIDNKNFNFVTEMFTSGSLREYRR 120
Query: 1091 KYKRVSIEAIKSWARQILQGLVFLHGHDPPVIHRDLKCDNIFVNGHLGQVKIGDLGLAAI 1270
KYKRVS++AIKSWARQILQGLV+LHGHDPPVIHRDLKCDNIFVNGHLGQVKIGDLGLAAI
Sbjct: 121 KYKRVSLQAIKSWARQILQGLVYLHGHDPPVIHRDLKCDNIFVNGHLGQVKIGDLGLAAI 180
Query: 1271 LRGSQSAHSVIGTPEFMAPELYEEEYNELADVYSFGMCVLEMLTSEYPYSECSNPAQIYK 1450
L+GSQSAHSVIGTPEFMAPE+YEEEYNELADVYSFGMCVLEMLTS+YPYSEC+NPAQIYK
Sbjct: 181 LQGSQSAHSVIGTPEFMAPEMYEEEYNELADVYSFGMCVLEMLTSDYPYSECTNPAQIYK 240
Query: 1451 KVTSGKLPLAFFRVEDVEAQKFIGKCLEPATKRPSAKDLLLDPFLVSDDSSSTMKFAIQK 1630
KVTSGKLP++FFR+ED EA++FIGKCLEPA RPSAKDLLL+PFL +DD+SS MK IQK
Sbjct: 241 KVTSGKLPMSFFRIEDGEARRFIGKCLEPAANRPSAKDLLLEPFLSTDDTSSAMKLKIQK 300
Query: 1631 PFLNDSEMEKLQLGDDFTRTEMKVIGKLNPEDETIFLKVQISDNNGSVRHVYFPFDIRTD 1810
PFLN++EMEKLQL D+F RTEMKVIGKLNPED+TIFLKVQISD SVR+VYFPFDI TD
Sbjct: 301 PFLNENEMEKLQLSDEFQRTEMKVIGKLNPEDDTIFLKVQISDKKCSVRNVYFPFDILTD 360
Query: 1811 TPIDVAMEMVKELEIADWDPFEIANMIDREISALLPHRWQNKCLDTFHTFSYKDDDGDEP 1990
TPIDVAMEMVKELEI+DWDPF+IANMI+REISALLPHRW+N D+FHTFSY+DDD DE
Sbjct: 361 TPIDVAMEMVKELEISDWDPFDIANMINREISALLPHRWKNDYSDSFHTFSYQDDDVDES 420
Query: 1991 HHHFRXXXXXXXXXXXIPDLVSKAEEISNRYYWLHDDLHDDNSSRCSSQGTYSNLNYYSV 2170
HFR IPD VSK+EEIS+ YY LHDDLHDDNSSRCSSQGTYSN NYYS+
Sbjct: 421 RLHFRSISSSSSLHESIPDFVSKSEEISHGYYLLHDDLHDDNSSRCSSQGTYSNWNYYSM 480
Query: 2171 DDHEYNVASIRKDKLPIIKSHKGTRFSPGEDLSNCNHCKTLVGSQTPSTSKSKMMMIDNR 2350
DDHE+N+ASIRKDKLPI+KSH NCNHCK + GSQ S+SKSKM+M +NR
Sbjct: 481 DDHEHNIASIRKDKLPIMKSH------------NCNHCKIIGGSQNCSSSKSKMVM-ENR 527
Query: 2351 RLTRNRSLIDIRSQLLHRSLVEEVNKRRQFNTVGAVENIGFQSPYD 2488
+LTRNRSLID RSQLLHRSLV+E+NKRRQ TVGAVENIGFQSPYD
Sbjct: 528 KLTRNRSLIDTRSQLLHRSLVDELNKRRQVKTVGAVENIGFQSPYD 573
>ref|NP_001236096.1| with no lysine kinase 13 [Glycine max] gi|225348655|gb|ACN87289.1|
with no lysine kinase [Glycine max]
Length = 550
Score = 825 bits (2132), Expect = 0.0
Identities = 429/571 (75%), Positives = 464/571 (81%), Gaps = 10/571 (1%)
Frame = +2
Query: 842 MKTVYKAIDEFLGIEVAWNQVRLNEVLRNPDDLQRLYSEVHLLSTLKHQSIIRFYTSWID 1021
MKTVYKAIDE LGIEVAWNQVRLNEVLR PDDLQRLYSEVHLLSTLKHQSI+RFYTSWID
Sbjct: 1 MKTVYKAIDEVLGIEVAWNQVRLNEVLRTPDDLQRLYSEVHLLSTLKHQSILRFYTSWID 60
Query: 1022 VDNKAFNFVTEMFTSGSLREYRKKYKRVSIEAIKSWARQILQGLVFLHGHDPPVIHRDLK 1201
+D++AFNF+TE FTSGSLRE DLK
Sbjct: 61 IDSRAFNFITEFFTSGSLRE-------------------------------------DLK 83
Query: 1202 CDNIFVNGHLGQVKIGDLGLAAILRGSQSAHSVIGTPEFMAPELYEEEYNELADVYSFGM 1381
CDNIFVNGHLGQVKIGDLGLAAIL GSQ AHSVIGTPEFMAPELYEEEYNELADVYSFGM
Sbjct: 84 CDNIFVNGHLGQVKIGDLGLAAILHGSQLAHSVIGTPEFMAPELYEEEYNELADVYSFGM 143
Query: 1382 CVLEMLTSEYPYSECSNPAQIYKKVTS------GKLPLAFFRVEDVEAQKFIGKCLEPAT 1543
CVLEMLTSEYPYSECSNPAQIYKKVTS GKLP+AFFR+ED+EAQ+FIGKCL PA
Sbjct: 144 CVLEMLTSEYPYSECSNPAQIYKKVTSVNSDSGGKLPMAFFRIEDMEAQRFIGKCLVPAE 203
Query: 1544 KRPSAKDLLLDPFLVSDDSSSTMKFAIQKPFLNDSEMEKLQLGDDFTRTEMKVIGKLNPE 1723
KRPSAK+LLLDPFLVSDD SSTMKFAIQKPFLN +EMEKLQL DD RT MKVIGKLNPE
Sbjct: 204 KRPSAKELLLDPFLVSDDPSSTMKFAIQKPFLNVNEMEKLQLSDDLPRTGMKVIGKLNPE 263
Query: 1724 DETIFLKVQISDNNGSVRHVYFPFDIRTDTPIDVAMEMVKELEIADWDPFEIANMIDREI 1903
++TIFLKVQISD +GSVR+V+FPFDI +DTPIDVA EMVKELEI D +P+EIANMIDREI
Sbjct: 264 NDTIFLKVQISDKDGSVRNVFFPFDILSDTPIDVATEMVKELEIEDGEPYEIANMIDREI 323
Query: 1904 SALLPHRWQNKCLDTFHTFSYKDDDGDE--PHHHFRXXXXXXXXXXXIPDLVSKAEEISN 2077
SALLPHR Q+ C D FHTF+Y DDD D+ PHHHFR + DLVSK EEIS+
Sbjct: 324 SALLPHRRQSSCSDAFHTFNYLDDDCDDDGPHHHFRSFSSSSSFQESMSDLVSKGEEISS 383
Query: 2078 RYYWLHDDLHDDNSSRCSSQGTYSNLNYYSVDDH-EYNVASIRKDKLPIIKSH-KGTRFS 2251
YYWLHDDLHDD SSRCSSQGTYSNLNYYSVDDH EYNV S+RKDKLPI SH KG + S
Sbjct: 384 GYYWLHDDLHDDTSSRCSSQGTYSNLNYYSVDDHQEYNVPSLRKDKLPITTSHNKGKKIS 443
Query: 2252 PGEDLSNCNHCKTLVGSQTPSTSKSKMMMIDNRRLTRNRSLIDIRSQLLHRSLVEEVNKR 2431
GEDLSN N K +VGSQ P TSKSKMM+ ++ RLTRNRSLIDIRSQLLHRSLVEEVNKR
Sbjct: 444 TGEDLSNFNQGKLMVGSQVPLTSKSKMMINNHHRLTRNRSLIDIRSQLLHRSLVEEVNKR 503
Query: 2432 RQFNTVGAVENIGFQSPYDASTK*SGHSLIS 2524
R F TVGAVENIGFQ+P D +TK S H+ ++
Sbjct: 504 RLFKTVGAVENIGFQAPCDVTTKWSQHACVA 534
>ref|XP_003553219.1| PREDICTED: probable serine/threonine-protein kinase WNK4-like
[Glycine max]
Length = 610
Score = 825 bits (2131), Expect = 0.0
Identities = 423/591 (71%), Positives = 471/591 (79%), Gaps = 1/591 (0%)
Frame = +2
Query: 731 MFSMRENKVDCKEDNCYVETDPTGRYGRFGDVLGKGAMKTVYKAIDEFLGIEVAWNQVRL 910
M+S + K D K + Y+ETDPTGRY R GD+LGKGAMKTVYKAIDE LGIEVAW+QV+L
Sbjct: 1 MYSNKRCKEDGKAEPRYIETDPTGRYARVGDILGKGAMKTVYKAIDEVLGIEVAWSQVKL 60
Query: 911 NEVLRNPDDLQRLYSEVHLLSTLKHQSIIRFYTSWIDVDNKAFNFVTEMFTSGSLREYRK 1090
NE LR P+DL+RLY EVHLLSTLKHQSI+RFYTSWIDVDNK FNF+TEMFTSG+LREYRK
Sbjct: 61 NEALRKPEDLERLYLEVHLLSTLKHQSIMRFYTSWIDVDNKTFNFITEMFTSGTLREYRK 120
Query: 1091 KYKRVSIEAIKSWARQILQGLVFLHGHDPPVIHRDLKCDNIFVNGHLGQVKIGDLGLAAI 1270
KYK + ++AIKSW R ILQGLV+LH HDPPVIHRDLKCDNIFVNGHLGQVKIGDLGLAAI
Sbjct: 121 KYKHIGLQAIKSWTRLILQGLVYLHEHDPPVIHRDLKCDNIFVNGHLGQVKIGDLGLAAI 180
Query: 1271 LRGSQSAHSVIGTPEFMAPELYEEEYNELADVYSFGMCVLEMLTSEYPYSECSNPAQIYK 1450
L GSQ AHSVIGTPEFMAPELYEEEYNEL DVYSFGMCVLEMLTS+YPYSEC+NPAQIYK
Sbjct: 181 LHGSQPAHSVIGTPEFMAPELYEEEYNELVDVYSFGMCVLEMLTSDYPYSECANPAQIYK 240
Query: 1451 KVTSGKLPLAFFRVEDVEAQKFIGKCLEPATKRPSAKDLLLDPFLVSDDSSSTMKFAIQK 1630
KVTSGKLP +FFR+ED EAQ+FIGKCL A KRPSAK+LL DPFL+SDD+SS K IQK
Sbjct: 241 KVTSGKLPASFFRIEDTEAQRFIGKCLITAAKRPSAKELLNDPFLLSDDASSMTKIGIQK 300
Query: 1631 PFLNDSEMEKLQLGDDFTRTEMKVIGKLNPEDETIFLKVQISDNNGSVRHVYFPFDIRTD 1810
PFLN +EMEKLQL D RTEM + GKLNPE +TIFLKVQISD +GS R+VYFPFDI TD
Sbjct: 301 PFLNYNEMEKLQLDDVSPRTEMSITGKLNPEHDTIFLKVQISDKDGSCRNVYFPFDIYTD 360
Query: 1811 TPIDVAMEMVKELEIADWDPFEIANMIDREISALLPHRWQNKCLDTFHTFSYKDDDGDEP 1990
TPIDVAMEMVKELEI D P +IANMI+ EIS LLP++ + C T +
Sbjct: 361 TPIDVAMEMVKELEITDLKPSDIANMIEGEISVLLPNKRNSNCSVITMTTT--------T 412
Query: 1991 HHHFRXXXXXXXXXXXIPDLVSKAEEISNRYYWLHDDLHDDNSSRCSSQGTYSNLNYYSV 2170
+HHF I S+A+++ N YWLH D+ DD SS CSS GTYSN N+ SV
Sbjct: 413 NHHFHSASSRSSSQGSISGSDSRADDLLNGDYWLHGDVLDDASSICSSHGTYSNSNFCSV 472
Query: 2171 DDHEYN-VASIRKDKLPIIKSHKGTRFSPGEDLSNCNHCKTLVGSQTPSTSKSKMMMIDN 2347
D+ E N AS RKDK IIKSH TRFSP ED N CK L G Q PSTSK+K MM DN
Sbjct: 473 DEQEENHKASTRKDKHLIIKSHMCTRFSPNEDPITLNQCKVLAGPQAPSTSKNKRMM-DN 531
Query: 2348 RRLTRNRSLIDIRSQLLHRSLVEEVNKRRQFNTVGAVENIGFQSPYDASTK 2500
RRLTRN+SLID RSQLLHRSLVEEVNKRR FNTVGAVENIGF +PY+ + K
Sbjct: 532 RRLTRNKSLIDTRSQLLHRSLVEEVNKRRLFNTVGAVENIGFVTPYEVTNK 582
>ref|XP_002319412.1| predicted protein [Populus trichocarpa] gi|222857788|gb|EEE95335.1|
predicted protein [Populus trichocarpa]
Length = 608
Score = 772 bits (1993), Expect = 0.0
Identities = 395/596 (66%), Positives = 467/596 (78%), Gaps = 15/596 (2%)
Frame = +2
Query: 758 DCKEDNCYVETDPTGRYGRFGDVLGKGAMKTVYKAIDEFLGIEVAWNQVRLNEVLRNPDD 937
+ K D+ Y ETDPTGRYGRF +VLGKGAMKTVYKAIDEFLGIEVAWN+V+LN+VL +PDD
Sbjct: 14 ETKGDHGYAETDPTGRYGRFEEVLGKGAMKTVYKAIDEFLGIEVAWNRVKLNQVLCSPDD 73
Query: 938 LQRLYSEVHLLSTLKHQSIIRFYTSWIDVDNKAFNFVTEMFTSGSLREYRKKYKRVSIEA 1117
LQRLYSEVHLLSTL H SII+FYTSWIDV K FNF+TEMFTSG+LREYRKKY RV+I A
Sbjct: 74 LQRLYSEVHLLSTLNHDSIIKFYTSWIDVRRKTFNFITEMFTSGTLREYRKKYTRVNIRA 133
Query: 1118 IKSWARQILQGLVFLHGHDPPVIHRDLKCDNIFVNGHLGQVKIGDLGLAAILRGSQSAHS 1297
IK WARQIL+G+V+LHGHDPPVIHRDLKCDNIFVNGHLGQVKIGDLGLAAILRGSQSAHS
Sbjct: 134 IKKWARQILEGIVYLHGHDPPVIHRDLKCDNIFVNGHLGQVKIGDLGLAAILRGSQSAHS 193
Query: 1298 VIGTPEFMAPELYEEEYNELADVYSFGMCVLEMLTSEYPYSECSNPAQIYKKVTSGKLPL 1477
VIGTPEFMAPELYEE YNEL DVYSFGMCVLEMLTSEYPYSEC+NPAQIYKKVTSGKLP
Sbjct: 194 VIGTPEFMAPELYEENYNELVDVYSFGMCVLEMLTSEYPYSECTNPAQIYKKVTSGKLPA 253
Query: 1478 AFFRVEDVEAQKFIGKCLEPATKRPSAKDLLLDPFLVSDDSS--STMKFAIQKPFLNDSE 1651
F R++D+EAQ+FIGKCL A KR SAK+LLLDPFL SD++ +F QKPFLND E
Sbjct: 254 VFHRIQDLEAQRFIGKCLVTAAKRLSAKELLLDPFLASDEAELPHVPRFRKQKPFLNDRE 313
Query: 1652 MEKLQLGDDFTRTEMKVIGKLNPEDETIFLKVQISDNNGSVRHVYFPFDIRTDTPIDVAM 1831
MEKLQL D RT+M + GKLNPED+TIFLKVQI++ +G++R+++FPFDI DTPIDVAM
Sbjct: 314 MEKLQLNDHPPRTDMTITGKLNPEDDTIFLKVQIANEDGTLRNIFFPFDILHDTPIDVAM 373
Query: 1832 EMVKELEIADWDPFEIANMIDREISALLPH--RWQNKCLDTFHTFSYKDDDGDEPHHHFR 2005
EMVKELEI DW+PFEIA+MID ISAL+P+ +W +++ HTF Y++DDG + H F
Sbjct: 374 EMVKELEIDDWEPFEIADMIDGAISALVPNWKKWDLPHIESHHTFDYQEDDGHD--HPFH 431
Query: 2006 XXXXXXXXXXXIPDLVSKAEEISNRYYWLHDDLHDDNSSRCSS-QGTYSNLNYYSVDDHE 2182
+ L+ + L DDL DD SS+ SS G+YS LNY S D+H+
Sbjct: 432 SSSSCSSSPASLSGLMP---------HLLQDDLFDDTSSQSSSHSGSYSCLNYISGDEHK 482
Query: 2183 YNVASIRKDKLPIIKSHKGTRFSPGEDLSN----------CNHCKTLVGSQTPSTSKSKM 2332
++++S R+DK I ++ TRF P E+ ++ NHCK L+ SQT ++S
Sbjct: 483 FDLSSTRRDKHLITRTQNSTRFCPQENSNSNIGQALATNAYNHCKVLLESQTRASSSKSK 542
Query: 2333 MMIDNRRLTRNRSLIDIRSQLLHRSLVEEVNKRRQFNTVGAVENIGFQSPYDASTK 2500
M+D+RRLTRNRSL+DIRSQLLHRSLVEEV+KRR TVG VE++GFQ P + K
Sbjct: 543 RMMDSRRLTRNRSLVDIRSQLLHRSLVEEVHKRRLSKTVGDVEDVGFQKPTEVCKK 598