BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Glycyrrhiza23_contig00000320
         (2384 letters)

Database: ./nr 
           23,641,837 sequences; 8,123,359,852 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

gb|AEK70969.1| isoprene synthase, partial [Wisteria sp. 101210T2...   560   e-157
gb|AEK70968.1| isoprene synthase, partial [Robinia pseudoacacia]      550   e-154
sp|Q6EJ97.1|ISPS_PUELO RecName: Full=Isoprene synthase, chloropl...   543   e-152
ref|XP_003556879.1| PREDICTED: isoprene synthase, chloroplastic-...   525   e-146
ref|XP_003533120.1| PREDICTED: LOW QUALITY PROTEIN: isoprene syn...   505   e-140

>gb|AEK70969.1| isoprene synthase, partial [Wisteria sp. 101210T2Dc1]
          Length = 541

 Score =  560 bits (1444), Expect = e-157
 Identities = 277/345 (80%), Positives = 308/345 (89%), Gaps = 6/345 (1%)
 Frame = -1

Query: 1280 IDKYQIKDPHVDGLLLELAKLDFNTVQLLQQKELRDLSKWWKEIGLASKLEFVRDRLMEV 1101
            IDK++IK+P  D LLLELAKLDFN VQ LQQKELRDLS+WWKEIGLA K+EFVRDRLMEV
Sbjct: 197  IDKFEIKEPQ-DRLLLELAKLDFNMVQTLQQKELRDLSRWWKEIGLARKMEFVRDRLMEV 255

Query: 1100 YFWAAGMAPDPQLSECRKAVTKMFGLVTIIDDVYDVYGTLDELQLFTDAVQRWDVNAVDT 921
            YFWA GMAPDP LS+CRKA+ KMFGLVTIIDDVYDVYGTLDELQLFTDAV+RWDVNA+DT
Sbjct: 256  YFWAVGMAPDPLLSDCRKAIAKMFGLVTIIDDVYDVYGTLDELQLFTDAVERWDVNALDT 315

Query: 920  LPDYMKLCFLALYNTVNDTAYSVLKEKGHNNLPYLAKSWCELCKAFMQEARWSNNKIIPE 741
            LPDYMKLCFLALYNTVNDTAYS+L+E+G N+LPYLAKSW ELCKAF+QEA+WSN K IPE
Sbjct: 316  LPDYMKLCFLALYNTVNDTAYSLLRERGDNSLPYLAKSWSELCKAFLQEAKWSNKKTIPE 375

Query: 740  FKEYLDNASVSSSGGALLTPCYFSLCTQN-DDTSQ-SPAALDSLTNFQGLVRSSCAIFRL 567
            F+EYLDNASVSSSGGALLTPCYFSL TQ+   TSQ   + +DSLTNF G+VRSSC IFRL
Sbjct: 376  FREYLDNASVSSSGGALLTPCYFSLLTQDVAVTSQFHSSTIDSLTNFHGVVRSSCTIFRL 435

Query: 566  CNDLATSTAELERGETTNAIASYMKEKGVGEERAREELGKLIDAEWKKMNGDGTV----F 399
            CNDLATS AELERGETTN+I SYM+EKGVGEE AREEL KLID EW K+N +  +    F
Sbjct: 436  CNDLATSAAELERGETTNSITSYMREKGVGEEEAREELSKLIDVEWMKLNRERVLDIGPF 495

Query: 398  PKAFKETAINMARVSHCTYQYGDGLGRPDDTAENRIKLLLIDPIP 264
            PKAF ETA+NMARVSHCTYQ+GDGLGRPD+TA+NRIKLLL++PIP
Sbjct: 496  PKAFMETAVNMARVSHCTYQHGDGLGRPDNTAQNRIKLLLLNPIP 540



 Score =  240 bits (613), Expect = 1e-60
 Identities = 126/174 (72%), Positives = 142/174 (81%), Gaps = 1/174 (0%)
 Frame = -3

Query: 2019 RRSANYQPNLWNFEFLQSQGNDLQVEILEERAGKLEEEVRGLI-DTTTEPLSLLEVIDKV 1843
            RRS NYQPNLWNF+FLQSQ NDL+ E+L+ERAGKLEEEVRGLI +  TEPLSLLE+ID V
Sbjct: 1    RRSGNYQPNLWNFDFLQSQKNDLKEEMLQERAGKLEEEVRGLINEVDTEPLSLLELIDNV 60

Query: 1842 HRFGLTYKFQHDINKALHRINVNSSDIYQGAEIEKSGSTLHATALAFRLLRQHGFEVSQD 1663
             R GLTYKFQ DINKAL RI          ++I KSG  LHA AL FRLLRQHGF++SQD
Sbjct: 61   ERLGLTYKFQEDINKALGRI--------VSSDINKSG--LHAAALTFRLLRQHGFQISQD 110

Query: 1662 VFESFKEKDGSFSDAVKGDAQGLLSLYEASYLGFEGEHVLDEAREFSTTHLKNI 1501
            VFE FK+K+G FS  +KGD QGLLSLYEASYLGFEGE+VL+EAR FSTTHL+NI
Sbjct: 111  VFEKFKDKEGRFSAEIKGDVQGLLSLYEASYLGFEGENVLEEARAFSTTHLRNI 164


>gb|AEK70968.1| isoprene synthase, partial [Robinia pseudoacacia]
          Length = 534

 Score =  550 bits (1416), Expect = e-154
 Identities = 270/343 (78%), Positives = 307/343 (89%), Gaps = 3/343 (0%)
 Frame = -1

Query: 1280 IDKYQIKDPHVDGLLLELAKLDFNTVQLLQQKELRDLSKWWKEIGLASKLEFVRDRLMEV 1101
            I+ Y++K+PH D LL+ELAKLDFN VQ LQ+KE+ +LS+WWKEIGL SKL+FVRDRL+EV
Sbjct: 198  IENYEVKEPH-DRLLVELAKLDFNMVQSLQKKEVGELSRWWKEIGLTSKLDFVRDRLVEV 256

Query: 1100 YFWAAGMAPDPQLSECRKAVTKMFGLVTIIDDVYDVYGTLDELQLFTDAVQRWDVNAVDT 921
            YFWA+GMAPDPQLSECRKAVTKMFGLVTIIDDVYDVYGTLDEL+LFT+AV+RWDVNAVDT
Sbjct: 257  YFWASGMAPDPQLSECRKAVTKMFGLVTIIDDVYDVYGTLDELELFTNAVERWDVNAVDT 316

Query: 920  LPDYMKLCFLALYNTVNDTAYSVLKEKGHNNLPYLAKSWCELCKAFMQEARWSNNKIIPE 741
            LPDYMKLCF ALYNTVNDTAY++LKEKG NNLPYLAKSW +LCKAF+QEA+WSNNKIIP 
Sbjct: 317  LPDYMKLCFFALYNTVNDTAYNLLKEKGDNNLPYLAKSWSDLCKAFLQEAKWSNNKIIPS 376

Query: 740  FKEYLDNASVSSSGGALLTPCYFSLCTQNDDTSQSPAALDSLTNFQGLVRSSCAIFRLCN 561
            F +Y++NASVSSSGGALLTPCYFS+  + D T+Q   ALDSLTN+ G VRSSCAIFRLCN
Sbjct: 377  FNKYIENASVSSSGGALLTPCYFSI--RQDITNQ---ALDSLTNYHGPVRSSCAIFRLCN 431

Query: 560  DLATSTAELERGETTNAIASYMKEKGVGEERAREELGKLIDAEWKKMNGD---GTVFPKA 390
            DLATS AELERGETTN+I SYM++ G+ EE+AR+EL  LIDAEWK++N +      FPKA
Sbjct: 432  DLATSAAELERGETTNSITSYMQDNGISEEQARDELRNLIDAEWKQINRERVFDQTFPKA 491

Query: 389  FKETAINMARVSHCTYQYGDGLGRPDDTAENRIKLLLIDPIPI 261
            F ETAINMARVSHCTYQYGDGLGRPD+TAENRIKLLLIDP PI
Sbjct: 492  FIETAINMARVSHCTYQYGDGLGRPDNTAENRIKLLLIDPFPI 534



 Score =  226 bits (576), Expect = 2e-56
 Identities = 125/173 (72%), Positives = 137/173 (79%), Gaps = 1/173 (0%)
 Frame = -3

Query: 2016 RSANYQPNLWNFEFLQSQGNDLQVEILEERAGKLEEEVRGLIDTTT-EPLSLLEVIDKVH 1840
            RSANYQPNLWNFEFLQSQ  DL VE L+ERA KLEEEVR LI+    EPL LLE++D V 
Sbjct: 1    RSANYQPNLWNFEFLQSQEYDLMVETLQERATKLEEEVRRLINRVDIEPLKLLELVDNVQ 60

Query: 1839 RFGLTYKFQHDINKALHRINVNSSDIYQGAEIEKSGSTLHATALAFRLLRQHGFEVSQDV 1660
            R GLTYKF+ DINKAL RI   S D     E EKSG  LHATAL FRLLRQHGFEVSQDV
Sbjct: 61   RLGLTYKFEDDINKALERIV--SLD-----EREKSG--LHATALIFRLLRQHGFEVSQDV 111

Query: 1659 FESFKEKDGSFSDAVKGDAQGLLSLYEASYLGFEGEHVLDEAREFSTTHLKNI 1501
            FES ++K+G F   +KGD QGLLSLYEASYLGFEGE++LDEAREFS THLKN+
Sbjct: 112  FESTRDKEGRFKAEIKGDVQGLLSLYEASYLGFEGENLLDEAREFSMTHLKNL 164


>sp|Q6EJ97.1|ISPS_PUELO RecName: Full=Isoprene synthase, chloroplastic; Flags: Precursor
            gi|35187004|gb|AAQ84170.1| isoprene synthase [Pueraria
            montana var. lobata]
          Length = 608

 Score =  543 bits (1400), Expect = e-152
 Identities = 272/346 (78%), Positives = 296/346 (85%), Gaps = 5/346 (1%)
 Frame = -1

Query: 1280 IDKYQIKDPHVDGLLLELAKLDFNTVQLLQQKELRDLSKWWKEIGLASKLEFVRDRLMEV 1101
            +DKY+ K+PH   LLLELAKLDFN VQ L QKEL+DLS+WW E+GLASKL+FVRDRLMEV
Sbjct: 261  LDKYEPKEPHHQ-LLLELAKLDFNMVQTLHQKELQDLSRWWTEMGLASKLDFVRDRLMEV 319

Query: 1100 YFWAAGMAPDPQLSECRKAVTKMFGLVTIIDDVYDVYGTLDELQLFTDAVQRWDVNAVDT 921
            YFWA GMAPDPQ  ECRKAVTKMFGLVTIIDDVYDVYGTLDELQLFTDAV+RWDVNA++T
Sbjct: 320  YFWALGMAPDPQFGECRKAVTKMFGLVTIIDDVYDVYGTLDELQLFTDAVERWDVNAINT 379

Query: 920  LPDYMKLCFLALYNTVNDTAYSVLKEKGHNNLPYLAKSWCELCKAFMQEARWSNNKIIPE 741
            LPDYMKLCFLALYNTVNDT+YS+LKEKGHNNL YL KSW ELCKAF+QEA+WSNNKIIP 
Sbjct: 380  LPDYMKLCFLALYNTVNDTSYSILKEKGHNNLSYLTKSWRELCKAFLQEAKWSNNKIIPA 439

Query: 740  FKEYLDNASVSSSGGALLTPCYFSLCTQNDDTSQSPAALDSLTNFQGLVRSSCAIFRLCN 561
            F +YL+NASVSSSG ALL P YFS+C Q +D S    AL SLT+F GLVRSSC IFRLCN
Sbjct: 440  FSKYLENASVSSSGVALLAPSYFSVCQQQEDISDH--ALRSLTDFHGLVRSSCVIFRLCN 497

Query: 560  DLATSTAELERGETTNAIASYMKEK-GVGEERAREELGKLIDAEWKKMN----GDGTVFP 396
            DLATS AELERGETTN+I SYM E  G  EE+AREEL KLIDAEWKKMN     D T+ P
Sbjct: 498  DLATSAAELERGETTNSIISYMHENDGTSEEQAREELRKLIDAEWKKMNRERVSDSTLLP 557

Query: 395  KAFKETAINMARVSHCTYQYGDGLGRPDDTAENRIKLLLIDPIPIH 258
            KAF E A+NMARVSHCTYQYGDGLGRPD   ENRIKLLLIDP PI+
Sbjct: 558  KAFMEIAVNMARVSHCTYQYGDGLGRPDYATENRIKLLLIDPFPIN 603



 Score =  258 bits (658), Expect = 7e-66
 Identities = 141/211 (66%), Positives = 163/211 (77%), Gaps = 1/211 (0%)
 Frame = -3

Query: 2112 QKASFLANPKPWRVTCAVGTQHSTQVTTQDTRRSANYQPNLWNFEFLQSQGNDLQVEILE 1933
            QK S LANPKPWRV CA  +Q  TQ+T  ++RRSANYQPNLWNFEFLQS  NDL+VE LE
Sbjct: 32   QKTS-LANPKPWRVICATSSQF-TQITEHNSRRSANYQPNLWNFEFLQSLENDLKVEKLE 89

Query: 1932 ERAGKLEEEVRGLIDTT-TEPLSLLEVIDKVHRFGLTYKFQHDINKALHRINVNSSDIYQ 1756
            E+A KLEEEVR +I+   T+PLSLLE+ID V R GLTYKF+ DI KAL  I +       
Sbjct: 90   EKATKLEEEVRCMINRVDTQPLSLLELIDDVQRLGLTYKFEKDIIKALENIVLLD----- 144

Query: 1755 GAEIEKSGSTLHATALAFRLLRQHGFEVSQDVFESFKEKDGSFSDAVKGDAQGLLSLYEA 1576
              E +K+ S LHATAL+FRLLRQHGFEVSQDVFE FK+K+G FS  +KGD QGLLSLYEA
Sbjct: 145  --ENKKNKSDLHATALSFRLLRQHGFEVSQDVFERFKDKEGGFSGELKGDVQGLLSLYEA 202

Query: 1575 SYLGFEGEHVLDEAREFSTTHLKNIVVNSIN 1483
            SYLGFEGE++L+EAR FS THLKN +   IN
Sbjct: 203  SYLGFEGENLLEEARTFSITHLKNNLKEGIN 233


>ref|XP_003556879.1| PREDICTED: isoprene synthase, chloroplastic-like [Glycine max]
          Length = 728

 Score =  525 bits (1352), Expect = e-146
 Identities = 263/345 (76%), Positives = 288/345 (83%), Gaps = 4/345 (1%)
 Frame = -1

Query: 1280 IDKYQIKDPHVDGLLLELAKLDFNTVQLLQQKELRDLSKWWKEIGLASKLEFVRDRLMEV 1101
            ++KY+ K+PH   LLLELAKLDFN VQLL QKEL++LS+WW E+GLASKLEF RDRLMEV
Sbjct: 387  LEKYEPKEPHHQ-LLLELAKLDFNMVQLLHQKELQELSRWWSEMGLASKLEFARDRLMEV 445

Query: 1100 YFWAAGMAPDPQLSECRKAVTKMFGLVTIIDDVYDVYGTLDELQLFTDAVQRWDVNAVDT 921
            YFWA GMAPDPQ  ECRKAVTKMFGLVTIIDDVYD+YGTLDELQLFTDAV+RWDVN V+T
Sbjct: 446  YFWALGMAPDPQFRECRKAVTKMFGLVTIIDDVYDIYGTLDELQLFTDAVERWDVNVVNT 505

Query: 920  LPDYMKLCFLALYNTVNDTAYSVLKEKGHNNLPYLAKSWCELCKAFMQEARWSNNKIIPE 741
            LPDYMKLC+LALYNTVNDTAYS+LKEKG NNL YL KSWCELCKAF+QEA+WSNNKI+P 
Sbjct: 506  LPDYMKLCYLALYNTVNDTAYSILKEKGRNNLSYLKKSWCELCKAFLQEAKWSNNKIVPA 565

Query: 740  FKEYLDNASVSSSGGALLTPCYFSLCTQNDDTSQSPAALDSLTNFQGLVRSSCAIFRLCN 561
            F +YL+NASVSSSG ALL P YFS+C Q  D S S   L  LTNF GLVRSSC IFRLCN
Sbjct: 566  FSKYLENASVSSSGVALLAPSYFSVC-QEQDISFSDKTLHYLTNFGGLVRSSCTIFRLCN 624

Query: 560  DLATSTAELERGETTNAIASYMKEKGVGEERAREELGKLIDAEWKKMN----GDGTVFPK 393
            DL TS AELERGETTN+I SYM E G  EE A EEL  LID EWKKMN     D T+ PK
Sbjct: 625  DLTTSAAELERGETTNSIMSYMHENGTSEEHACEELRNLIDIEWKKMNRQRVSDSTL-PK 683

Query: 392  AFKETAINMARVSHCTYQYGDGLGRPDDTAENRIKLLLIDPIPIH 258
            AF+E A+NMARVSH TYQYGDGLGRPD   ENRIK LLIDP+PI+
Sbjct: 684  AFREIAMNMARVSHNTYQYGDGLGRPDYNIENRIKFLLIDPVPIN 728



 Score =  221 bits (562), Expect = 9e-55
 Identities = 128/203 (63%), Positives = 153/203 (75%), Gaps = 2/203 (0%)
 Frame = -3

Query: 2076 RVTCAVGTQHSTQVTTQDTRRSANYQPNLWNFEFLQ-SQGNDLQVEILEERAGKLEEEVR 1900
            R+ CAV +Q  TQ+T  D+RRSANYQPNLWNFEFL  S  ND +VE LEERA K+EEEVR
Sbjct: 169  RLICAVSSQF-TQITDDDSRRSANYQPNLWNFEFLPPSLENDHKVEKLEERAKKVEEEVR 227

Query: 1899 GLID-TTTEPLSLLEVIDKVHRFGLTYKFQHDINKALHRINVNSSDIYQGAEIEKSGSTL 1723
             +I+   T+PL LLE+ID V   GLTYKF+ DI KAL +I   S D     E E+  S L
Sbjct: 228  KVINGIDTKPL-LLELIDDVQHLGLTYKFEKDIIKALEKIV--SLD-----ENEEHKSEL 279

Query: 1722 HATALAFRLLRQHGFEVSQDVFESFKEKDGSFSDAVKGDAQGLLSLYEASYLGFEGEHVL 1543
            + TAL+FRLLRQHGFEVSQDVF+ FK+K+G FS  +KGD QGLLSLYEASYLGFEG+++L
Sbjct: 280  YYTALSFRLLRQHGFEVSQDVFKRFKDKEGGFSGELKGDVQGLLSLYEASYLGFEGDNLL 339

Query: 1542 DEAREFSTTHLKNIVVNSINLQQ 1474
            DEAR FSTTHLKN +   IN ++
Sbjct: 340  DEARAFSTTHLKNNLKQGINTKE 362


>ref|XP_003533120.1| PREDICTED: LOW QUALITY PROTEIN: isoprene synthase, chloroplastic-like
            [Glycine max]
          Length = 835

 Score =  505 bits (1300), Expect = e-140
 Identities = 249/344 (72%), Positives = 284/344 (82%), Gaps = 3/344 (0%)
 Frame = -1

Query: 1280 IDKYQIKDPHVDGLLLELAKLDFNTVQLLQQKELRDLSKWWKEIGLASKLEFVRDRLMEV 1101
            ++KY+  + H   +LLELAK+DFN VQ++ QKELR+LS+WW E+GL SKL+FVRDRLMEV
Sbjct: 262  LEKYEPNESH-HHVLLELAKIDFNLVQVMYQKELRELSRWWSEMGLTSKLKFVRDRLMEV 320

Query: 1100 YFWAAGMAPDPQLSECRKAVTKMFGLVTIIDDVYDVYGTLDELQLFTDAVQRWDVNAVDT 921
            YFW  GMAP PQ SECRKAVTK F L+ IIDDVYDVYGTLDELQLFTDA++RWDVNA++T
Sbjct: 321  YFWVLGMAPRPQFSECRKAVTKTFALIGIIDDVYDVYGTLDELQLFTDAIERWDVNAMNT 380

Query: 920  LPDYMKLCFLALYNTVNDTAYSVLKEKGHNNLPYLAKSWCELCKAFMQEARWSNNKIIPE 741
            LPDYMKLC+LA+YNTVNDT YS LK KGHNN+ YL KSWCELCKAF+QEA+WSNNKI+P 
Sbjct: 381  LPDYMKLCYLAVYNTVNDTCYSTLKAKGHNNMSYLTKSWCELCKAFLQEAKWSNNKIVPT 440

Query: 740  FKEYLDNASVSSSGGALLTPCYFSLCTQNDDTSQSPAALDSLTNFQGLVRSSCAIFRLCN 561
            F +YL+NASVSSSG ALLT  YFS+C Q D ++Q   AL SLTNFQGLVRSS  IFRLCN
Sbjct: 441  FSKYLENASVSSSGMALLTASYFSVCQQQDISNQQ--ALCSLTNFQGLVRSSSNIFRLCN 498

Query: 560  DLATSTAELERGETTNAIASYMKEKGVGEERAREELGKLIDAEWKKMNGD---GTVFPKA 390
            DLATS AELE GET N+I  YM EK   EE+AREEL  LIDAEWKKMN +    +  PKA
Sbjct: 499  DLATSAAELETGETANSITCYMHEKDTSEEQAREELTNLIDAEWKKMNREFVSNSTLPKA 558

Query: 389  FKETAINMARVSHCTYQYGDGLGRPDDTAENRIKLLLIDPIPIH 258
            FKE AINMARVSHC YQY DGLGRP  T EN+IKLLLIDP+PI+
Sbjct: 559  FKEIAINMARVSHCMYQYEDGLGRPGYTTENKIKLLLIDPVPIN 602



 Score =  227 bits (578), Expect = 1e-56
 Identities = 128/211 (60%), Positives = 155/211 (73%), Gaps = 4/211 (1%)
 Frame = -3

Query: 2097 LANPKPWRVTCAVGTQHSTQVTTQDTRRSANYQPNLWNFEFLQSQGNDL---QVEILEER 1927
            LANP PW+V CA+ +Q  TQ+T  + RRSANYQ + W FEFLQ  GND+   Q+E LEER
Sbjct: 34   LANPNPWQVICAMSSQF-TQMTQHNGRRSANYQQSHWKFEFLQCLGNDVKHVQMENLEER 92

Query: 1926 AGKLEEEVRGLID-TTTEPLSLLEVIDKVHRFGLTYKFQHDINKALHRINVNSSDIYQGA 1750
            A K+EEEVR +I+   TE L LLE+ID++ R GLTYKF+ DI KAL +    S D     
Sbjct: 93   ARKVEEEVRRMINGADTEALRLLELIDEIQRLGLTYKFEKDIFKALEK--TISLD----- 145

Query: 1749 EIEKSGSTLHATALAFRLLRQHGFEVSQDVFESFKEKDGSFSDAVKGDAQGLLSLYEASY 1570
            E EK  S LHATAL+FRLLRQHGFEVSQDVF+ FK+K+G F + +KGD QGLLSLYEASY
Sbjct: 146  ENEKHISGLHATALSFRLLRQHGFEVSQDVFKRFKDKEGGFINELKGDMQGLLSLYEASY 205

Query: 1569 LGFEGEHVLDEAREFSTTHLKNIVVNSINLQ 1477
            LGFEGE +LDEAR +S THLKN +   +N +
Sbjct: 206  LGFEGETLLDEARAYSITHLKNNLKVGVNTE 236