BLASTX nr result

ID: Gardenia21_contig00010500 seq

BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Gardenia21_contig00010500
         (338 letters)

Database: ./nr 
           77,306,371 sequences; 28,104,191,420 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CDP02894.1| unnamed protein product [Coffea canephora]            190   4e-46
ref|XP_009790991.1| PREDICTED: WAT1-related protein At4g30420 [N...   119   1e-24
ref|XP_009587592.1| PREDICTED: WAT1-related protein At4g30420 [N...   117   3e-24
ref|XP_011079339.1| PREDICTED: WAT1-related protein At4g30420-li...   116   6e-24
ref|XP_012841042.1| PREDICTED: WAT1-related protein At4g30420-li...   115   1e-23
gb|EYU34299.1| hypothetical protein MIMGU_mgv1a026334mg [Erythra...   115   1e-23
ref|XP_012071606.1| PREDICTED: WAT1-related protein At4g30420-li...   110   3e-22
ref|XP_010323229.1| PREDICTED: WAT1-related protein At4g30420 is...   109   7e-22
ref|XP_004242999.1| PREDICTED: WAT1-related protein At4g30420 is...   109   7e-22
ref|XP_007013956.1| Nodulin MtN21 /EamA-like transporter family ...   108   2e-21
ref|XP_006343720.1| PREDICTED: WAT1-related protein At4g30420-li...   106   8e-21
ref|XP_012071607.1| PREDICTED: WAT1-related protein At4g30420-li...   105   1e-20
gb|KDP38760.1| hypothetical protein JCGZ_04113 [Jatropha curcas]      105   1e-20
ref|XP_011091589.1| PREDICTED: WAT1-related protein At4g30420-li...   105   1e-20
ref|XP_002528210.1| Auxin-induced protein 5NG4, putative [Ricinu...   105   1e-20
emb|CBI36607.3| unnamed protein product [Vitis vinifera]              105   2e-20
ref|XP_002528209.1| conserved hypothetical protein [Ricinus comm...   105   2e-20
ref|XP_002324505.1| hypothetical protein POPTR_0018s10840g [Popu...   104   3e-20
ref|XP_002309381.2| hypothetical protein POPTR_0006s19150g, part...   103   5e-20
ref|XP_013750373.1| PREDICTED: WAT1-related protein At4g28040-li...   102   9e-20

>emb|CDP02894.1| unnamed protein product [Coffea canephora]
          Length = 379

 Score =  190 bits (482), Expect = 4e-46
 Identities = 91/111 (81%), Positives = 97/111 (87%)
 Frame = -3

Query: 336 WKLQTPLELGCLFFTAGASAISFFGQAWCICKRGPLFSAMFNPLGXXXXXXXXXXXFREE 157
           WKLQTPLELGCLFFTAGASAISFF QAWCICKRGPLFSAMFNPL            FREE
Sbjct: 241 WKLQTPLELGCLFFTAGASAISFFAQAWCICKRGPLFSAMFNPLSTVIVTFIAFTFFREE 300

Query: 156 MYVGSMLGAIAVIFGLYIVLWGKAKDHLINSDVEKKPEEEKINCTIIIDES 4
           +Y+GS+LGAIAVIFGLYIVLWGKAKDH+INSDVEKKPEEEKIN TI++DES
Sbjct: 301 LYIGSLLGAIAVIFGLYIVLWGKAKDHMINSDVEKKPEEEKINYTIVVDES 351


>ref|XP_009790991.1| PREDICTED: WAT1-related protein At4g30420 [Nicotiana sylvestris]
          Length = 375

 Score =  119 bits (297), Expect = 1e-24
 Identities = 56/107 (52%), Positives = 76/107 (71%)
 Frame = -3

Query: 336 WKLQTPLELGCLFFTAGASAISFFGQAWCICKRGPLFSAMFNPLGXXXXXXXXXXXFREE 157
           WK+ + LE   +F+T  +SA+SFFGQAWCI +RGPLFSAMFNPL             +EE
Sbjct: 242 WKVTSSLEFISIFYTGFSSAVSFFGQAWCISRRGPLFSAMFNPLCTVIVTIFASAFMQEE 301

Query: 156 MYVGSMLGAIAVIFGLYIVLWGKAKDHLINSDVEKKPEEEKINCTII 16
           MY GS++G++AVIFGLY+VLWGKAKD    + +E++P +++I  T I
Sbjct: 302 MYTGSLVGSLAVIFGLYVVLWGKAKDKKEENIIEEEPVKQQIQETTI 348


>ref|XP_009587592.1| PREDICTED: WAT1-related protein At4g30420 [Nicotiana
           tomentosiformis]
          Length = 381

 Score =  117 bits (294), Expect = 3e-24
 Identities = 56/107 (52%), Positives = 76/107 (71%)
 Frame = -3

Query: 336 WKLQTPLELGCLFFTAGASAISFFGQAWCICKRGPLFSAMFNPLGXXXXXXXXXXXFREE 157
           WK+ + LE   +F+T  +SA+SFFGQAWCI +RGPLFSAMFNPL             +EE
Sbjct: 242 WKVTSSLEFISIFYTGFSSAVSFFGQAWCISRRGPLFSAMFNPLCTVIVTIIASAFMQEE 301

Query: 156 MYVGSMLGAIAVIFGLYIVLWGKAKDHLINSDVEKKPEEEKINCTII 16
           MY GS++G++AVIFGLY+VLWGKAKD   ++ +E++P ++ I  T I
Sbjct: 302 MYTGSLVGSLAVIFGLYVVLWGKAKDKKEDNILEEEPVKQHIQETTI 348


>ref|XP_011079339.1| PREDICTED: WAT1-related protein At4g30420-like [Sesamum indicum]
          Length = 369

 Score =  116 bits (291), Expect = 6e-24
 Identities = 56/100 (56%), Positives = 69/100 (69%)
 Frame = -3

Query: 336 WKLQTPLELGCLFFTAGASAISFFGQAWCICKRGPLFSAMFNPLGXXXXXXXXXXXFREE 157
           W L++PL+L   FFT  ASA++FF QAWCI KRGPLFSAMFNPL              EE
Sbjct: 237 WTLKSPLQLFSCFFTGLASAVTFFAQAWCIAKRGPLFSAMFNPLCTVIVTVFACIFMHEE 296

Query: 156 MYVGSMLGAIAVIFGLYIVLWGKAKDHLINSDVEKKPEEE 37
           +Y GSM+G +AVI GLYIVLWGKAKD+   ++  KKP ++
Sbjct: 297 LYTGSMIGGLAVIVGLYIVLWGKAKDYEEVAEETKKPSDQ 336


>ref|XP_012841042.1| PREDICTED: WAT1-related protein At4g30420-like [Erythranthe
           guttatus]
          Length = 360

 Score =  115 bits (289), Expect = 1e-23
 Identities = 56/111 (50%), Positives = 71/111 (63%), Gaps = 2/111 (1%)
 Frame = -3

Query: 336 WKLQTPLELGCLFFTAGASAISFFGQAWCICKRGPLFSAMFNPLGXXXXXXXXXXXFREE 157
           WKL +PL+L C F+    SA++FF QAWCI +RGPLFSAMFNPL              EE
Sbjct: 237 WKLTSPLQLFCCFYAGLGSAVTFFAQAWCIARRGPLFSAMFNPLCTVIVTVFACVFVHEE 296

Query: 156 MYVGSMLGAIAVIFGLYIVLWGKAKDH--LINSDVEKKPEEEKINCTIIID 10
           +Y GS+LG +AVI GLY+VLWGKAKDH  +I+ D   +   +    + IID
Sbjct: 297 LYTGSLLGGLAVIVGLYVVLWGKAKDHEDVISEDQSSQLASDNSTISCIID 347


>gb|EYU34299.1| hypothetical protein MIMGU_mgv1a026334mg [Erythranthe guttata]
          Length = 357

 Score =  115 bits (289), Expect = 1e-23
 Identities = 56/111 (50%), Positives = 71/111 (63%), Gaps = 2/111 (1%)
 Frame = -3

Query: 336 WKLQTPLELGCLFFTAGASAISFFGQAWCICKRGPLFSAMFNPLGXXXXXXXXXXXFREE 157
           WKL +PL+L C F+    SA++FF QAWCI +RGPLFSAMFNPL              EE
Sbjct: 234 WKLTSPLQLFCCFYAGLGSAVTFFAQAWCIARRGPLFSAMFNPLCTVIVTVFACVFVHEE 293

Query: 156 MYVGSMLGAIAVIFGLYIVLWGKAKDH--LINSDVEKKPEEEKINCTIIID 10
           +Y GS+LG +AVI GLY+VLWGKAKDH  +I+ D   +   +    + IID
Sbjct: 294 LYTGSLLGGLAVIVGLYVVLWGKAKDHEDVISEDQSSQLASDNSTISCIID 344


>ref|XP_012071606.1| PREDICTED: WAT1-related protein At4g30420-like [Jatropha curcas]
           gi|643731471|gb|KDP38759.1| hypothetical protein
           JCGZ_04112 [Jatropha curcas]
          Length = 372

 Score =  110 bits (276), Expect = 3e-22
 Identities = 59/111 (53%), Positives = 69/111 (62%)
 Frame = -3

Query: 336 WKLQTPLELGCLFFTAGASAISFFGQAWCICKRGPLFSAMFNPLGXXXXXXXXXXXFREE 157
           WKL + LE+ C  +     A+SFFGQAWCI +RGPLFSAMFNPL              EE
Sbjct: 238 WKLNSYLEICCCLYAGVGLALSFFGQAWCISQRGPLFSAMFNPLCTVIVTIIAALFLHEE 297

Query: 156 MYVGSMLGAIAVIFGLYIVLWGKAKDHLINSDVEKKPEEEKINCTIIIDES 4
            Y+GS +G +AVI GLY+VLWGKAKD     D E K EE  I   +IIDES
Sbjct: 298 TYLGSFIGGLAVIIGLYVVLWGKAKDF----DEEIKKEESSI-VKVIIDES 343


>ref|XP_010323229.1| PREDICTED: WAT1-related protein At4g30420 isoform X2 [Solanum
           lycopersicum]
          Length = 386

 Score =  109 bits (273), Expect = 7e-22
 Identities = 58/114 (50%), Positives = 75/114 (65%), Gaps = 3/114 (2%)
 Frame = -3

Query: 336 WKLQTPLELGCLFFTAGASAISFFGQAWCICKRGPLFSAMFNPLGXXXXXXXXXXXFREE 157
           WK+ + LEL    +T  +SA+SFFGQAWCI  RGPLFSAMFNPL             +EE
Sbjct: 245 WKINSSLELISCLYTGFSSAVSFFGQAWCISHRGPLFSAMFNPLCTVIVTIFASTFMKEE 304

Query: 156 MYVGSMLGAIAVIFGLYIVLWGKAKDHL-INSDVEKKPEEEK--INCTIIIDES 4
           +Y GS++G++AVIFGLY+VLWGK+KD    N  V+K+P ++      TIII  S
Sbjct: 305 LYTGSLVGSLAVIFGLYVVLWGKSKDKKEENIIVDKEPVKQHNIQETTIIIQNS 358


>ref|XP_004242999.1| PREDICTED: WAT1-related protein At4g30420 isoform X1 [Solanum
           lycopersicum]
          Length = 387

 Score =  109 bits (273), Expect = 7e-22
 Identities = 58/114 (50%), Positives = 75/114 (65%), Gaps = 3/114 (2%)
 Frame = -3

Query: 336 WKLQTPLELGCLFFTAGASAISFFGQAWCICKRGPLFSAMFNPLGXXXXXXXXXXXFREE 157
           WK+ + LEL    +T  +SA+SFFGQAWCI  RGPLFSAMFNPL             +EE
Sbjct: 246 WKINSSLELISCLYTGFSSAVSFFGQAWCISHRGPLFSAMFNPLCTVIVTIFASTFMKEE 305

Query: 156 MYVGSMLGAIAVIFGLYIVLWGKAKDHL-INSDVEKKPEEEK--INCTIIIDES 4
           +Y GS++G++AVIFGLY+VLWGK+KD    N  V+K+P ++      TIII  S
Sbjct: 306 LYTGSLVGSLAVIFGLYVVLWGKSKDKKEENIIVDKEPVKQHNIQETTIIIQNS 359


>ref|XP_007013956.1| Nodulin MtN21 /EamA-like transporter family protein, putative
           [Theobroma cacao] gi|508784319|gb|EOY31575.1| Nodulin
           MtN21 /EamA-like transporter family protein, putative
           [Theobroma cacao]
          Length = 379

 Score =  108 bits (270), Expect = 2e-21
 Identities = 58/114 (50%), Positives = 70/114 (61%), Gaps = 2/114 (1%)
 Frame = -3

Query: 336 WKLQTPLELGCLFFTAGASAISFFGQAWCICKRGPLFSAMFNPLGXXXXXXXXXXXFREE 157
           W L + LEL C  FT  A A+SFF QAWCI + GPLFSAMFNPL              EE
Sbjct: 238 WSLNSYLELSCCLFTGIALAVSFFVQAWCISRTGPLFSAMFNPLCTVIVTIVAAVFQHEE 297

Query: 156 MYVGSMLGAIAVIFGLYIVLWGKAKD-HLINSDVEKKPEEEKINCT-IIIDESA 1
            Y+GS++GA AVI GLYIVLWGKAKD   I   ++ KP   +     +I+DES+
Sbjct: 298 TYIGSLVGAFAVIIGLYIVLWGKAKDVEEIKEGMDPKPLNNQTKIVQVIMDESS 351


>ref|XP_006343720.1| PREDICTED: WAT1-related protein At4g30420-like [Solanum tuberosum]
          Length = 386

 Score =  106 bits (264), Expect = 8e-21
 Identities = 49/86 (56%), Positives = 61/86 (70%)
 Frame = -3

Query: 336 WKLQTPLELGCLFFTAGASAISFFGQAWCICKRGPLFSAMFNPLGXXXXXXXXXXXFREE 157
           WK+ + LEL    +   +SA+SFFGQAWCI  RGPLFSAMFNPL             +EE
Sbjct: 245 WKITSSLELISCLYAGFSSAVSFFGQAWCISHRGPLFSAMFNPLCTVIVTIFASTFMKEE 304

Query: 156 MYVGSMLGAIAVIFGLYIVLWGKAKD 79
           MY GS++G++AVIFGLY+VLWGK+KD
Sbjct: 305 MYTGSLVGSLAVIFGLYVVLWGKSKD 330


>ref|XP_012071607.1| PREDICTED: WAT1-related protein At4g30420-like [Jatropha curcas]
          Length = 366

 Score =  105 bits (263), Expect = 1e-20
 Identities = 56/114 (49%), Positives = 71/114 (62%), Gaps = 4/114 (3%)
 Frame = -3

Query: 336 WKLQTPLELGCLFFTA-GASAISFFGQAWCICKRGPLFSAMFNPLGXXXXXXXXXXXFRE 160
           WKL + LEL C  F    ASA+SFF QAWC+ +RGPLF+AMFNPL              E
Sbjct: 229 WKLHSYLELSCCLFAGIVASALSFFAQAWCVSQRGPLFTAMFNPLCTVIVTVFAALFLHE 288

Query: 159 EMYVGSMLGAIAVIFGLYIVLWGKAKDHLINSDVEKKPE---EEKINCTIIIDE 7
           E+Y+GS+LG + VI GLYI+LWGKAKD   N + E  P+   ++  N   +IDE
Sbjct: 289 EIYIGSLLGGVGVIIGLYILLWGKAKDFRKNGE-EVDPKLGIDQMQNANTLIDE 341


>gb|KDP38760.1| hypothetical protein JCGZ_04113 [Jatropha curcas]
          Length = 160

 Score =  105 bits (263), Expect = 1e-20
 Identities = 56/114 (49%), Positives = 71/114 (62%), Gaps = 4/114 (3%)
 Frame = -3

Query: 336 WKLQTPLELGCLFFTA-GASAISFFGQAWCICKRGPLFSAMFNPLGXXXXXXXXXXXFRE 160
           WKL + LEL C  F    ASA+SFF QAWC+ +RGPLF+AMFNPL              E
Sbjct: 23  WKLHSYLELSCCLFAGIVASALSFFAQAWCVSQRGPLFTAMFNPLCTVIVTVFAALFLHE 82

Query: 159 EMYVGSMLGAIAVIFGLYIVLWGKAKDHLINSDVEKKPE---EEKINCTIIIDE 7
           E+Y+GS+LG + VI GLYI+LWGKAKD   N + E  P+   ++  N   +IDE
Sbjct: 83  EIYIGSLLGGVGVIIGLYILLWGKAKDFRKNGE-EVDPKLGIDQMQNANTLIDE 135


>ref|XP_011091589.1| PREDICTED: WAT1-related protein At4g30420-like [Sesamum indicum]
          Length = 360

 Score =  105 bits (262), Expect = 1e-20
 Identities = 48/87 (55%), Positives = 58/87 (66%)
 Frame = -3

Query: 336 WKLQTPLELGCLFFTAGASAISFFGQAWCICKRGPLFSAMFNPLGXXXXXXXXXXXFREE 157
           W L +P +L C FF    +A++F GQ+WCI +RGPLFSAMF PL              EE
Sbjct: 239 WNLNSPFQLFCCFFGGLGAAVTFLGQSWCIERRGPLFSAMFQPLSTVIVTLFACLFQHEE 298

Query: 156 MYVGSMLGAIAVIFGLYIVLWGKAKDH 76
           MY+GS +G +AVI GLYIVLWGKAKDH
Sbjct: 299 MYMGSFIGTLAVITGLYIVLWGKAKDH 325


>ref|XP_002528210.1| Auxin-induced protein 5NG4, putative [Ricinus communis]
           gi|223532371|gb|EEF34167.1| Auxin-induced protein 5NG4,
           putative [Ricinus communis]
          Length = 380

 Score =  105 bits (262), Expect = 1e-20
 Identities = 49/86 (56%), Positives = 59/86 (68%)
 Frame = -3

Query: 336 WKLQTPLELGCLFFTAGASAISFFGQAWCICKRGPLFSAMFNPLGXXXXXXXXXXXFREE 157
           WKL + LE+GC  +     A+SFF QAWCI +RGPLFSAMFNPL              EE
Sbjct: 238 WKLNSYLEMGCCLYAGVGLAVSFFLQAWCISQRGPLFSAMFNPLCTVITAIIAAIFLHEE 297

Query: 156 MYVGSMLGAIAVIFGLYIVLWGKAKD 79
            Y+GS++GA+AVI GLY+VLWGKAKD
Sbjct: 298 TYLGSLIGALAVIIGLYVVLWGKAKD 323


>emb|CBI36607.3| unnamed protein product [Vitis vinifera]
          Length = 268

 Score =  105 bits (261), Expect = 2e-20
 Identities = 55/115 (47%), Positives = 72/115 (62%), Gaps = 3/115 (2%)
 Frame = -3

Query: 336 WKLQTPLELGCLFFTAGASAISFFGQAWCICKRGPLFSAMFNPLGXXXXXXXXXXXFREE 157
           W L++  EL    ++A  S +++F QAWCI +RGPLFSAMFNPL              EE
Sbjct: 136 WILRSKFELVRCLYSASRSGLNYFLQAWCISRRGPLFSAMFNPLCTVIVTILDPLSLHEE 195

Query: 156 MYVGSMLGAIAVIFGLYIVLWGKAKDHLINSDVEKKPE---EEKINCTIIIDESA 1
           +Y+GS++GA+AVI GLY+VLWGKAKD L  S     PE    E  N  ++IDES+
Sbjct: 196 LYIGSLVGAVAVIIGLYVVLWGKAKD-LEESQTVSNPELQNNEAKNVRVLIDESS 249


>ref|XP_002528209.1| conserved hypothetical protein [Ricinus communis]
           gi|223532370|gb|EEF34166.1| conserved hypothetical
           protein [Ricinus communis]
          Length = 258

 Score =  105 bits (261), Expect = 2e-20
 Identities = 56/115 (48%), Positives = 76/115 (66%), Gaps = 4/115 (3%)
 Frame = -3

Query: 336 WKLQTPLEL-GCLFFTAGASAISFFGQAWCICKRGPLFSAMFNPLGXXXXXXXXXXXFRE 160
           WKL + LEL  CLF    +S +SFF QAWCI +RGPLF+AMFNPLG             E
Sbjct: 113 WKLHSYLELICCLFAGIVSSGLSFFLQAWCISQRGPLFTAMFNPLGTVIVTVCAAMFLHE 172

Query: 159 EMYVGSMLGAIAVIFGLYIVLWGKAKDHLINSDVEKKPE---EEKINCTIIIDES 4
           E+Y+GS++GA+ VI GLY+VLW KAKD ++ ++ +K P+   ++     I+IDES
Sbjct: 173 EIYMGSLIGAVGVIIGLYVVLWSKAKD-VVRNEEDKDPKLKTDQMHISNILIDES 226


>ref|XP_002324505.1| hypothetical protein POPTR_0018s10840g [Populus trichocarpa]
           gi|222865939|gb|EEF03070.1| hypothetical protein
           POPTR_0018s10840g [Populus trichocarpa]
          Length = 371

 Score =  104 bits (259), Expect = 3e-20
 Identities = 61/114 (53%), Positives = 72/114 (63%), Gaps = 3/114 (2%)
 Frame = -3

Query: 336 WKLQTPLEL-GCLFFTAGASAISFFGQAWCICKRGPLFSAMFNPLGXXXXXXXXXXXFRE 160
           WKL + LE+ GCLF     S ISFF QAW I +RGPLFSAMFNPL              E
Sbjct: 230 WKLHSYLEVVGCLFTGIIGSGISFFVQAWVISQRGPLFSAMFNPLCTVIVTILAAIFLHE 289

Query: 159 EMYVGSMLGAIAVIFGLYIVLWGKAKDHLINSD-VEKKPE-EEKINCTIIIDES 4
           E+Y GS++GA+AVI GLYIVLWGKAKD     D V+ K E +E+    I I+ES
Sbjct: 290 EIYTGSLIGAVAVIIGLYIVLWGKAKDFTKEEDKVDPKLEIDERQTVKITIEES 343


>ref|XP_002309381.2| hypothetical protein POPTR_0006s19150g, partial [Populus
           trichocarpa] gi|550336637|gb|EEE92904.2| hypothetical
           protein POPTR_0006s19150g, partial [Populus trichocarpa]
          Length = 212

 Score =  103 bits (257), Expect = 5e-20
 Identities = 56/111 (50%), Positives = 69/111 (62%), Gaps = 1/111 (0%)
 Frame = -3

Query: 336 WKLQTPLEL-GCLFFTAGASAISFFGQAWCICKRGPLFSAMFNPLGXXXXXXXXXXXFRE 160
           WKL + LEL GCLF     S +SFF QAW IC+RGPLFSAMFNPL              E
Sbjct: 82  WKLHSYLELVGCLFTGIIGSGLSFFVQAWVICQRGPLFSAMFNPLCTVIVTVLAAIFLHE 141

Query: 159 EMYVGSMLGAIAVIFGLYIVLWGKAKDHLINSDVEKKPEEEKINCTIIIDE 7
           E+Y G ++G +AVI GLYIVLWGKAKD +         EE++I+  + ID+
Sbjct: 142 EIYTGGLIGGVAVIIGLYIVLWGKAKDFI--------KEEDEIDPKLEIDQ 184


>ref|XP_013750373.1| PREDICTED: WAT1-related protein At4g28040-like [Brassica napus]
          Length = 361

 Score =  102 bits (255), Expect = 9e-20
 Identities = 46/100 (46%), Positives = 65/100 (65%)
 Frame = -3

Query: 336 WKLQTPLELGCLFFTAGASAISFFGQAWCICKRGPLFSAMFNPLGXXXXXXXXXXXFREE 157
           WKL + L+L C  ++    A+SFF QAWC+ ++GPLFSA+FNPL             +E+
Sbjct: 232 WKLDSSLKLSCCIYSGLQLAVSFFLQAWCVSRKGPLFSALFNPLSTVIVTFFGALYLKEQ 291

Query: 156 MYVGSMLGAIAVIFGLYIVLWGKAKDHLINSDVEKKPEEE 37
            Y+GS+LGA+A+I GLYIVLWGK +D+L     + K + E
Sbjct: 292 TYLGSLLGALAIILGLYIVLWGKTEDYLQEEATDLKLQNE 331


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