BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Forsythia23_contig00043262
         (311 letters)

Database: ./nr 
           69,698,275 sequences; 24,982,196,650 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_012857391.1| PREDICTED: Werner Syndrome-like exonuclease ...   155   8e-36
ref|XP_011079095.1| PREDICTED: Werner Syndrome-like exonuclease ...   147   2e-33
emb|CDP06274.1| unnamed protein product [Coffea canephora]            139   6e-31
ref|XP_012828040.1| PREDICTED: Werner Syndrome-like exonuclease ...   131   2e-28
ref|XP_011040699.1| PREDICTED: Werner Syndrome-like exonuclease ...   129   6e-28
ref|XP_011070070.1| PREDICTED: Werner Syndrome-like exonuclease ...   129   1e-27
ref|XP_011070071.1| PREDICTED: Werner Syndrome-like exonuclease ...   127   4e-27
ref|XP_002527682.1| 3-5 exonuclease, putative [Ricinus communis]...   126   5e-27
ref|XP_002323207.1| hypothetical protein POPTR_0016s02850g [Popu...   126   6e-27
ref|XP_008244081.1| PREDICTED: Werner Syndrome-like exonuclease ...   125   8e-27
ref|XP_011041345.1| PREDICTED: Werner Syndrome-like exonuclease ...   125   1e-26
ref|XP_002308852.1| hypothetical protein POPTR_0006s02990g [Popu...   125   1e-26
ref|XP_012080224.1| PREDICTED: Werner Syndrome-like exonuclease ...   124   2e-26
ref|XP_006342546.1| PREDICTED: Werner Syndrome-like exonuclease-...   124   2e-26
ref|XP_010111231.1| Werner Syndrome-like exonuclease [Morus nota...   123   5e-26
ref|XP_009785659.1| PREDICTED: Werner Syndrome-like exonuclease ...   123   5e-26
ref|XP_009602248.1| PREDICTED: Werner Syndrome-like exonuclease ...   122   7e-26
ref|XP_007140430.1| hypothetical protein PHAVU_008G111200g [Phas...   122   7e-26
ref|XP_007202594.1| hypothetical protein PRUPE_ppa011620mg [Prun...   122   9e-26
ref|XP_006482020.1| PREDICTED: Werner Syndrome-like exonuclease-...   122   1e-25

>ref|XP_012857391.1| PREDICTED: Werner Syndrome-like exonuclease [Erythranthe guttatus]
           gi|604301106|gb|EYU20826.1| hypothetical protein
           MIMGU_mgv1a013022mg [Erythranthe guttata]
          Length = 233

 Score =  155 bits (393), Expect = 8e-36
 Identities = 73/103 (70%), Positives = 85/103 (82%)
 Frame = -3

Query: 309 HSPSVPPPLIDFLSNQNYTFVGIGIEADLEKLEEDYEFGYSANAVDLRKLAADTYQMREL 130
           H+PS+P  LI FL+N NYTFVGIGI+AD+EKLEEDYEFG++ N VDLR  AA  Y  REL
Sbjct: 101 HAPSIPSALIGFLANPNYTFVGIGIKADIEKLEEDYEFGFNTNTVDLRNTAAAAYGRREL 160

Query: 129 KNAGLKSLARIVLGKEVEKPNRVTMSRWDYRWLTPAQVQYACV 1
            N+GLKSLA  VLG+E+EKP RVTMSRWD +WLTP QVQYAC+
Sbjct: 161 LNSGLKSLALTVLGRELEKPKRVTMSRWDNQWLTPPQVQYACI 203


>ref|XP_011079095.1| PREDICTED: Werner Syndrome-like exonuclease [Sesamum indicum]
          Length = 222

 Score =  147 bits (372), Expect = 2e-33
 Identities = 73/103 (70%), Positives = 81/103 (78%)
 Frame = -3

Query: 309 HSPSVPPPLIDFLSNQNYTFVGIGIEADLEKLEEDYEFGYSANAVDLRKLAADTYQMREL 130
           HSP +P  +IDFL+N +YTFVGIGIE DLEKLE DYE G +   VDLR LAAD Y  REL
Sbjct: 101 HSPFIPSAIIDFLANPHYTFVGIGIEQDLEKLE-DYELGLNTKTVDLRSLAADEYGRREL 159

Query: 129 KNAGLKSLARIVLGKEVEKPNRVTMSRWDYRWLTPAQVQYACV 1
           K+AGLK LA +VL KEVEKP RVTMSRWD  WLTP QVQYAC+
Sbjct: 160 KSAGLKQLASLVLEKEVEKPKRVTMSRWDNEWLTPPQVQYACI 202


>emb|CDP06274.1| unnamed protein product [Coffea canephora]
          Length = 217

 Score =  139 bits (351), Expect = 6e-31
 Identities = 66/99 (66%), Positives = 78/99 (78%)
 Frame = -3

Query: 297 VPPPLIDFLSNQNYTFVGIGIEADLEKLEEDYEFGYSANAVDLRKLAADTYQMRELKNAG 118
           +P  L DFL N +YTFVGIG+E+D+EKL EDY  G   + VDLR LAA+   M++L+NAG
Sbjct: 102 IPDDLRDFLGNTDYTFVGIGVESDVEKLLEDYGLGVGGSVVDLRYLAAEECGMKQLRNAG 161

Query: 117 LKSLARIVLGKEVEKPNRVTMSRWDYRWLTPAQVQYACV 1
           LK LAR+VLG+EV KP RVTM RWDYRWLTP QVQYACV
Sbjct: 162 LKELARVVLGREVGKPRRVTMGRWDYRWLTPDQVQYACV 200


>ref|XP_012828040.1| PREDICTED: Werner Syndrome-like exonuclease [Erythranthe guttatus]
           gi|604298580|gb|EYU18582.1| hypothetical protein
           MIMGU_mgv1a013807mg [Erythranthe guttata]
          Length = 210

 Score =  131 bits (330), Expect = 2e-28
 Identities = 61/103 (59%), Positives = 81/103 (78%)
 Frame = -3

Query: 309 HSPSVPPPLIDFLSNQNYTFVGIGIEADLEKLEEDYEFGYSANAVDLRKLAADTYQMREL 130
           HSP +P  L  FLS  ++TFVG+ +++DLEKLE DY+ GY+A AVDLR +AA+ Y+ ++L
Sbjct: 90  HSPYLPIQLTRFLSFSHHTFVGVEVKSDLEKLERDYKIGYNARAVDLRGVAAEVYRRKDL 149

Query: 129 KNAGLKSLARIVLGKEVEKPNRVTMSRWDYRWLTPAQVQYACV 1
           K AGLK LA+IV+GKE++KP  VTMS WD RWLT  Q++YACV
Sbjct: 150 KCAGLKGLAKIVIGKEMDKPKSVTMSNWDNRWLTADQIKYACV 192


>ref|XP_011040699.1| PREDICTED: Werner Syndrome-like exonuclease [Populus euphratica]
          Length = 215

 Score =  129 bits (325), Expect = 6e-28
 Identities = 64/104 (61%), Positives = 79/104 (75%), Gaps = 1/104 (0%)
 Frame = -3

Query: 309 HSPS-VPPPLIDFLSNQNYTFVGIGIEADLEKLEEDYEFGYSANAVDLRKLAADTYQMRE 133
           H P+ +P  L DFLSN+NYTFVG+GIE D+EKL EDY+     NAVDLR LAA+     +
Sbjct: 98  HCPTGIPQSLYDFLSNKNYTFVGVGIEGDVEKLVEDYDVSMG-NAVDLRVLAAEKLGAEK 156

Query: 132 LKNAGLKSLARIVLGKEVEKPNRVTMSRWDYRWLTPAQVQYACV 1
            KN+G+KSL + +LGK++EKP RVTMSRWD  WLT AQVQYAC+
Sbjct: 157 WKNSGIKSLVKEILGKQIEKPKRVTMSRWDNEWLTGAQVQYACL 200


>ref|XP_011070070.1| PREDICTED: Werner Syndrome-like exonuclease [Sesamum indicum]
          Length = 213

 Score =  129 bits (323), Expect = 1e-27
 Identities = 61/103 (59%), Positives = 78/103 (75%)
 Frame = -3

Query: 309 HSPSVPPPLIDFLSNQNYTFVGIGIEADLEKLEEDYEFGYSANAVDLRKLAADTYQMREL 130
           +S  +P  L+DFLS +++TFVG+GI++DLEKL++DY  G  A+AV+L K AAD Y  +EL
Sbjct: 94  YSRYIPESLVDFLSEEDFTFVGVGIKSDLEKLDKDYGIGGDAHAVELGKRAADEYDRKEL 153

Query: 129 KNAGLKSLARIVLGKEVEKPNRVTMSRWDYRWLTPAQVQYACV 1
           KN GLK L+  VL +E+EKP RVTM RWD RWLT  QV YACV
Sbjct: 154 KNTGLKGLSSFVLRRELEKPKRVTMGRWDNRWLTEDQVLYACV 196


>ref|XP_011070071.1| PREDICTED: Werner Syndrome-like exonuclease [Sesamum indicum]
          Length = 210

 Score =  127 bits (318), Expect = 4e-27
 Identities = 64/103 (62%), Positives = 76/103 (73%)
 Frame = -3

Query: 309 HSPSVPPPLIDFLSNQNYTFVGIGIEADLEKLEEDYEFGYSANAVDLRKLAADTYQMREL 130
           HSP  P  LI FLS  N+TFVG+ +++DL+KL+ D+  GY+A AVDLR LAAD   M+EL
Sbjct: 87  HSPYFPLQLIQFLSFSNHTFVGVDVKSDLKKLQRDHRIGYNARAVDLRGLAADVCGMKEL 146

Query: 129 KNAGLKSLARIVLGKEVEKPNRVTMSRWDYRWLTPAQVQYACV 1
           K  GL+SL R VLGKE+EKP  VTMS WD R LT  QVQYACV
Sbjct: 147 KKGGLRSLTRSVLGKEMEKPKWVTMSSWDNRVLTADQVQYACV 189


>ref|XP_002527682.1| 3-5 exonuclease, putative [Ricinus communis]
           gi|223532913|gb|EEF34681.1| 3-5 exonuclease, putative
           [Ricinus communis]
          Length = 211

 Score =  126 bits (317), Expect = 5e-27
 Identities = 59/103 (57%), Positives = 77/103 (74%)
 Frame = -3

Query: 309 HSPSVPPPLIDFLSNQNYTFVGIGIEADLEKLEEDYEFGYSANAVDLRKLAADTYQMREL 130
           HSP +P  L DFL N +Y F G+GIE+D+EKL EDY      N ++LR++AA++  ++EL
Sbjct: 93  HSPRIPQSLFDFLKNSSYVFAGVGIESDVEKLVEDYGLSVG-NVMELRRVAAESLGVKEL 151

Query: 129 KNAGLKSLARIVLGKEVEKPNRVTMSRWDYRWLTPAQVQYACV 1
           KNAGLK L + VLGKE++KP RVTMSRWD  WL+  QVQYAC+
Sbjct: 152 KNAGLKELVKQVLGKEIQKPKRVTMSRWDSMWLSHDQVQYACL 194


>ref|XP_002323207.1| hypothetical protein POPTR_0016s02850g [Populus trichocarpa]
           gi|222867837|gb|EEF04968.1| hypothetical protein
           POPTR_0016s02850g [Populus trichocarpa]
          Length = 215

 Score =  126 bits (316), Expect = 6e-27
 Identities = 64/104 (61%), Positives = 77/104 (74%), Gaps = 1/104 (0%)
 Frame = -3

Query: 309 HSPS-VPPPLIDFLSNQNYTFVGIGIEADLEKLEEDYEFGYSANAVDLRKLAADTYQMRE 133
           H P+ +P  L DFLS+  YTFVG+GIE D++KL EDYE     NAVDLR LAA+      
Sbjct: 98  HCPTGIPQSLHDFLSDMTYTFVGVGIEGDVKKLTEDYELSVG-NAVDLRGLAAEKLGDSR 156

Query: 132 LKNAGLKSLARIVLGKEVEKPNRVTMSRWDYRWLTPAQVQYACV 1
            KN+G+K LAR VLGKE+EKP R+T+SRWD  WLTPAQVQYAC+
Sbjct: 157 WKNSGVKRLAREVLGKEIEKPKRITLSRWDNPWLTPAQVQYACL 200


>ref|XP_008244081.1| PREDICTED: Werner Syndrome-like exonuclease [Prunus mume]
          Length = 203

 Score =  125 bits (315), Expect = 8e-27
 Identities = 64/103 (62%), Positives = 78/103 (75%)
 Frame = -3

Query: 309 HSPSVPPPLIDFLSNQNYTFVGIGIEADLEKLEEDYEFGYSANAVDLRKLAADTYQMREL 130
           H+  +P  L  FL+N++YTFVG+GI +D+EKL  DY   + ANAVDL   AAD    REL
Sbjct: 88  HTQHIPQALYAFLANRSYTFVGVGIGSDVEKLLLDYGL-HVANAVDLAVFAADRIGSREL 146

Query: 129 KNAGLKSLARIVLGKEVEKPNRVTMSRWDYRWLTPAQVQYACV 1
           +NAGLK LAR +LGKEV+KPNR+TMSRWD +WLT  QVQYACV
Sbjct: 147 RNAGLKGLARQMLGKEVQKPNRITMSRWDNQWLTCDQVQYACV 189


>ref|XP_011041345.1| PREDICTED: Werner Syndrome-like exonuclease [Populus euphratica]
          Length = 252

 Score =  125 bits (313), Expect = 1e-26
 Identities = 62/99 (62%), Positives = 75/99 (75%)
 Frame = -3

Query: 297 VPPPLIDFLSNQNYTFVGIGIEADLEKLEEDYEFGYSANAVDLRKLAADTYQMRELKNAG 118
           +P  L DFLS+  YTFVG+GIE+D++KL EDYE     NAVDLR LAA+       KN+G
Sbjct: 140 IPQSLHDFLSDMTYTFVGVGIESDVKKLTEDYELSVG-NAVDLRGLAAEKLGDSRWKNSG 198

Query: 117 LKSLARIVLGKEVEKPNRVTMSRWDYRWLTPAQVQYACV 1
           +K LAR VLGKE+EKP R+T+SRWD  WLTPAQVQYAC+
Sbjct: 199 VKRLAREVLGKEIEKPKRITLSRWDNVWLTPAQVQYACL 237


>ref|XP_002308852.1| hypothetical protein POPTR_0006s02990g [Populus trichocarpa]
           gi|222854828|gb|EEE92375.1| hypothetical protein
           POPTR_0006s02990g [Populus trichocarpa]
          Length = 215

 Score =  125 bits (313), Expect = 1e-26
 Identities = 62/104 (59%), Positives = 77/104 (74%), Gaps = 1/104 (0%)
 Frame = -3

Query: 309 HSPS-VPPPLIDFLSNQNYTFVGIGIEADLEKLEEDYEFGYSANAVDLRKLAADTYQMRE 133
           H P+ +P  L DFLSN+NYTFVG+GIE D+EKL E Y+     NAVDLR LAA+     +
Sbjct: 98  HCPTGIPQSLYDFLSNKNYTFVGVGIEGDVEKLVEGYDVSMG-NAVDLRVLAAEKLGAEQ 156

Query: 132 LKNAGLKSLARIVLGKEVEKPNRVTMSRWDYRWLTPAQVQYACV 1
            KN+G+KSL + +LGK++EKP RVTMSRWD  WLT  QVQYAC+
Sbjct: 157 WKNSGIKSLVKEILGKQIEKPKRVTMSRWDNEWLTGDQVQYACL 200


>ref|XP_012080224.1| PREDICTED: Werner Syndrome-like exonuclease [Jatropha curcas]
           gi|643741786|gb|KDP47148.1| hypothetical protein
           JCGZ_00039 [Jatropha curcas]
          Length = 212

 Score =  124 bits (312), Expect = 2e-26
 Identities = 62/103 (60%), Positives = 72/103 (69%)
 Frame = -3

Query: 309 HSPSVPPPLIDFLSNQNYTFVGIGIEADLEKLEEDYEFGYSANAVDLRKLAADTYQMREL 130
           HSPSVP  L+DFL   N+ FVG+GI +D EKL EDY      N VDLR LAA    + EL
Sbjct: 96  HSPSVPQSLVDFLQGGNFVFVGVGIRSDTEKLLEDYGLNVG-NQVDLRGLAAQRLGLTEL 154

Query: 129 KNAGLKSLARIVLGKEVEKPNRVTMSRWDYRWLTPAQVQYACV 1
           + A LK LAR VLG E+EKP RV +SRWD+ WLTP QVQYAC+
Sbjct: 155 RGASLKVLARRVLGLEIEKPRRVKLSRWDHMWLTPVQVQYACI 197


>ref|XP_006342546.1| PREDICTED: Werner Syndrome-like exonuclease-like [Solanum
           tuberosum]
          Length = 220

 Score =  124 bits (312), Expect = 2e-26
 Identities = 61/100 (61%), Positives = 72/100 (72%)
 Frame = -3

Query: 300 SVPPPLIDFLSNQNYTFVGIGIEADLEKLEEDYEFGYSANAVDLRKLAADTYQMRELKNA 121
           S+P  L +FL N+ Y FVGIGIE+D+EKL EDYE     N VDLR +AAD Y M+ LKNA
Sbjct: 96  SIPESLFNFLGNREYDFVGIGIESDVEKLLEDYELNVK-NVVDLRGIAADVYGMKNLKNA 154

Query: 120 GLKSLARIVLGKEVEKPNRVTMSRWDYRWLTPAQVQYACV 1
           GLK L  +VLGKE+ KP  VTM RWD  WL+  QVQYAC+
Sbjct: 155 GLKELCNVVLGKEIVKPKSVTMGRWDSEWLSLNQVQYACL 194


>ref|XP_010111231.1| Werner Syndrome-like exonuclease [Morus notabilis]
           gi|587944227|gb|EXC30709.1| Werner Syndrome-like
           exonuclease [Morus notabilis]
          Length = 211

 Score =  123 bits (308), Expect = 5e-26
 Identities = 63/103 (61%), Positives = 73/103 (70%)
 Frame = -3

Query: 309 HSPSVPPPLIDFLSNQNYTFVGIGIEADLEKLEEDYEFGYSANAVDLRKLAADTYQMREL 130
           H+ S+P  L DFL+N NY+FVG+GI AD+EKL ED+      NAVDL  LAA  Y    +
Sbjct: 94  HADSIPASLSDFLANANYSFVGVGIGADIEKLAEDHGLTVR-NAVDLGDLAAREYDDPGM 152

Query: 129 KNAGLKSLARIVLGKEVEKPNRVTMSRWDYRWLTPAQVQYACV 1
           KN GLK LARIV G E+EKP R+TM RWD RWLT  QVQYACV
Sbjct: 153 KNRGLKDLARIVRGMEIEKPKRITMGRWDNRWLTCEQVQYACV 195


>ref|XP_009785659.1| PREDICTED: Werner Syndrome-like exonuclease [Nicotiana sylvestris]
          Length = 213

 Score =  123 bits (308), Expect = 5e-26
 Identities = 58/100 (58%), Positives = 73/100 (73%)
 Frame = -3

Query: 300 SVPPPLIDFLSNQNYTFVGIGIEADLEKLEEDYEFGYSANAVDLRKLAADTYQMRELKNA 121
           S+P  L+ FLSN +YTFVG+G+E D+EKL ED+E     N VDLR LAAD Y+M  LK A
Sbjct: 97  SIPDSLVSFLSNGDYTFVGVGVENDVEKLVEDHELTVR-NMVDLRVLAADAYEMSNLKTA 155

Query: 120 GLKSLARIVLGKEVEKPNRVTMSRWDYRWLTPAQVQYACV 1
           GLK L  +VLG+E+EKP  +TM RWD  WL+  Q++YACV
Sbjct: 156 GLKELCNVVLGREIEKPRHITMGRWDSEWLSLDQIKYACV 195


>ref|XP_009602248.1| PREDICTED: Werner Syndrome-like exonuclease [Nicotiana
           tomentosiformis]
          Length = 213

 Score =  122 bits (307), Expect = 7e-26
 Identities = 57/100 (57%), Positives = 73/100 (73%)
 Frame = -3

Query: 300 SVPPPLIDFLSNQNYTFVGIGIEADLEKLEEDYEFGYSANAVDLRKLAADTYQMRELKNA 121
           S+P  L+ FLSN +YTFVG+G+E D+EKL ED++     N VDLR LAAD Y+M  LK A
Sbjct: 97  SIPDSLVSFLSNGDYTFVGVGVENDVEKLVEDHDLSVR-NMVDLRVLAADAYEMSNLKTA 155

Query: 120 GLKSLARIVLGKEVEKPNRVTMSRWDYRWLTPAQVQYACV 1
           GLK L  +VLG+++EKP  +TM RWD  WL+  Q+QYACV
Sbjct: 156 GLKELCNVVLGRQIEKPRSITMGRWDSEWLSLDQIQYACV 195


>ref|XP_007140430.1| hypothetical protein PHAVU_008G111200g [Phaseolus vulgaris]
           gi|561013563|gb|ESW12424.1| hypothetical protein
           PHAVU_008G111200g [Phaseolus vulgaris]
          Length = 227

 Score =  122 bits (307), Expect = 7e-26
 Identities = 64/103 (62%), Positives = 71/103 (68%)
 Frame = -3

Query: 309 HSPSVPPPLIDFLSNQNYTFVGIGIEADLEKLEEDYEFGYSANAVDLRKLAADTYQMREL 130
           HSPS+PP L+ FL++ N TFVG+GIE D EKL EDY   +  N  DLR LAA     REL
Sbjct: 110 HSPSIPPSLVSFLADPNITFVGVGIEEDAEKLLEDYNL-HVVNVRDLRSLAAQKLGDREL 168

Query: 129 KNAGLKSLARIVLGKEVEKPNRVTMSRWDYRWLTPAQVQYACV 1
             AG+KSL   VLG EVEKP RVT SRWD  WLTP QVQYA V
Sbjct: 169 NQAGIKSLGLRVLGLEVEKPKRVTRSRWDNPWLTPVQVQYATV 211


>ref|XP_007202594.1| hypothetical protein PRUPE_ppa011620mg [Prunus persica]
           gi|462398125|gb|EMJ03793.1| hypothetical protein
           PRUPE_ppa011620mg [Prunus persica]
          Length = 203

 Score =  122 bits (306), Expect = 9e-26
 Identities = 62/103 (60%), Positives = 77/103 (74%)
 Frame = -3

Query: 309 HSPSVPPPLIDFLSNQNYTFVGIGIEADLEKLEEDYEFGYSANAVDLRKLAADTYQMREL 130
           H+  +P  L  FL+N++YTFVG+GI +D+EKL  DY   + ANAVDL   AA  +   EL
Sbjct: 88  HTDHIPQALHAFLANRSYTFVGVGIGSDVEKLLLDYGL-HVANAVDLAVFAAGRFDSTEL 146

Query: 129 KNAGLKSLARIVLGKEVEKPNRVTMSRWDYRWLTPAQVQYACV 1
           +NAGLK LAR +LGKEV+KPNR+TMSRWD +WLT  QVQYACV
Sbjct: 147 RNAGLKGLARRMLGKEVQKPNRITMSRWDNQWLTCDQVQYACV 189


>ref|XP_006482020.1| PREDICTED: Werner Syndrome-like exonuclease-like [Citrus sinensis]
          Length = 209

 Score =  122 bits (305), Expect = 1e-25
 Identities = 59/103 (57%), Positives = 74/103 (71%)
 Frame = -3

Query: 309 HSPSVPPPLIDFLSNQNYTFVGIGIEADLEKLEEDYEFGYSANAVDLRKLAADTYQMREL 130
           H+ ++P  L+ FLS + Y+FVG+GI+ DLEKLE+ Y      N V+L +LA D     +L
Sbjct: 93  HAQTIPRSLVGFLSTECYSFVGVGIKKDLEKLEDFYGITVQGNVVELGRLAGDRKGRTDL 152

Query: 129 KNAGLKSLARIVLGKEVEKPNRVTMSRWDYRWLTPAQVQYACV 1
            NAGLK+LAR VLG + EKP RVTMSRWD RWL PAQVQYAC+
Sbjct: 153 VNAGLKNLAREVLGLDFEKPRRVTMSRWDKRWLDPAQVQYACI 195