BLASTX nr result
ID: Forsythia23_contig00038649
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Forsythia23_contig00038649
(1294 letters)
Database: ./nr
69,698,275 sequences; 24,982,196,650 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|KHN13885.1| hypothetical protein glysoja_016169 [Glycine soja] 97 2e-17
ref|XP_006603031.1| PREDICTED: uncharacterized protein LOC102660... 97 2e-17
ref|XP_006603030.1| PREDICTED: uncharacterized protein LOC102660... 97 2e-17
ref|XP_009347444.1| PREDICTED: uncharacterized protein LOC103939... 94 2e-16
ref|XP_009347443.1| PREDICTED: uncharacterized protein LOC103939... 94 2e-16
ref|XP_009347442.1| PREDICTED: uncharacterized protein LOC103939... 94 2e-16
ref|XP_009357694.1| PREDICTED: uncharacterized protein LOC103948... 94 2e-16
ref|XP_010319262.1| PREDICTED: uncharacterized protein LOC101263... 92 1e-15
ref|XP_004236756.1| PREDICTED: uncharacterized protein LOC101263... 92 1e-15
ref|XP_006365712.1| PREDICTED: uncharacterized protein LOC102590... 91 2e-15
ref|XP_008233589.1| PREDICTED: uncharacterized protein LOC103332... 91 3e-15
ref|XP_012568897.1| PREDICTED: uncharacterized protein LOC101514... 87 4e-14
ref|XP_012568896.1| PREDICTED: uncharacterized protein LOC101514... 87 4e-14
ref|XP_004492099.1| PREDICTED: uncharacterized protein LOC101514... 87 4e-14
ref|XP_009600592.1| PREDICTED: uncharacterized protein LOC104096... 84 2e-13
ref|XP_009600591.1| PREDICTED: uncharacterized protein LOC104096... 84 2e-13
ref|XP_009798390.1| PREDICTED: uncharacterized protein LOC104244... 83 5e-13
ref|XP_009798389.1| PREDICTED: uncharacterized protein LOC104244... 83 5e-13
ref|XP_010658598.1| PREDICTED: uncharacterized protein LOC100253... 82 1e-12
ref|XP_007009394.1| Zinc finger C3H1 domain-containing protein, ... 80 4e-12
>gb|KHN13885.1| hypothetical protein glysoja_016169 [Glycine soja]
Length = 2747
Score = 97.4 bits (241), Expect = 2e-17
Identities = 76/257 (29%), Positives = 116/257 (45%), Gaps = 26/257 (10%)
Frame = -3
Query: 713 AVPKVMPRKASLSRTFVSSKNKIHGATSG-------------------NRSSSGPDHLPK 591
++PK +P++ SLSRTFVSS KI G+ S N++ + +
Sbjct: 1223 SLPKEVPKRLSLSRTFVSSLTKIPGSNSKGVGSVPLVQGSRARNFNPVNKNLANRERGRD 1282
Query: 590 TNVHLNTTKLQDLRQMIAIRENEXXXXXXXXXXXXXXXSHGTCDAMNFANDMGKQAALA- 414
V N KLQDLRQ IA+RE+E A+N N K ++
Sbjct: 1283 QGVVSNDNKLQDLRQQIALRESELKLKAAQQNKESASVLGRDHSAINSKNMARKSTPVSS 1342
Query: 413 -----EPKEPEKKRLKITEPRQNTMGASGQEVMPPVESMLASKNPLLENDHQQILENCCH 249
EPKEP++KRLK++ + Q+ +P V+S+L K+ LEN H Q H
Sbjct: 1343 GPAQLEPKEPDRKRLKVSTSYGTSQAVDSQQEVPVVKSLLPPKDSTLENYHPQERNKIDH 1402
Query: 248 HNGKSKPGTSHTIHAKGQEQNENVVPVSSKNLLVGTKEGSGTTRC-QTEKKSKLAYSSIA 72
+ + Q+Q + + S +N+ +++G G C QTEK S+L S+A
Sbjct: 1403 GKKEIPLCRAEPKTITSQKQPDKHLDNSLENMPRRSRDGDGNYGCNQTEKSSRLVDPSVA 1462
Query: 71 SEQNMQMANKDAETILK 21
QN AN + ++ K
Sbjct: 1463 FNQNALPANMSSNSVPK 1479
>ref|XP_006603031.1| PREDICTED: uncharacterized protein LOC102660840 isoform X2 [Glycine
max]
Length = 1384
Score = 97.4 bits (241), Expect = 2e-17
Identities = 76/257 (29%), Positives = 116/257 (45%), Gaps = 26/257 (10%)
Frame = -3
Query: 713 AVPKVMPRKASLSRTFVSSKNKIHGATSG-------------------NRSSSGPDHLPK 591
++PK +P++ SLSRTFVSS KI G+ S N++ + +
Sbjct: 156 SLPKEVPKRLSLSRTFVSSLTKIPGSNSKGVGSVPLVQGSRARNFNPVNKNLANRERGRD 215
Query: 590 TNVHLNTTKLQDLRQMIAIRENEXXXXXXXXXXXXXXXSHGTCDAMNFANDMGKQAALA- 414
V N KLQDLRQ IA+RE+E A+N N K ++
Sbjct: 216 QGVVSNDNKLQDLRQQIALRESELKLKAAQQNKESASVLGRDHSAINSKNMARKSTPVSS 275
Query: 413 -----EPKEPEKKRLKITEPRQNTMGASGQEVMPPVESMLASKNPLLENDHQQILENCCH 249
EPKEP++KRLK++ + Q+ +P V+S+L K+ LEN H Q H
Sbjct: 276 GPAQLEPKEPDRKRLKVSTSYGTSQAVDSQQEVPVVKSLLPPKDSTLENYHPQERNKIDH 335
Query: 248 HNGKSKPGTSHTIHAKGQEQNENVVPVSSKNLLVGTKEGSGTTRC-QTEKKSKLAYSSIA 72
+ + Q+Q + + S +N+ +++G G C QTEK S+L S+A
Sbjct: 336 GKKEIPLCRAEPKTITSQKQPDKHLDNSLENMPRRSRDGDGNYGCNQTEKSSRLVDPSVA 395
Query: 71 SEQNMQMANKDAETILK 21
QN AN + ++ K
Sbjct: 396 FNQNALPANMSSNSVPK 412
>ref|XP_006603030.1| PREDICTED: uncharacterized protein LOC102660840 isoform X1 [Glycine
max]
Length = 1680
Score = 97.4 bits (241), Expect = 2e-17
Identities = 76/257 (29%), Positives = 116/257 (45%), Gaps = 26/257 (10%)
Frame = -3
Query: 713 AVPKVMPRKASLSRTFVSSKNKIHGATSG-------------------NRSSSGPDHLPK 591
++PK +P++ SLSRTFVSS KI G+ S N++ + +
Sbjct: 156 SLPKEVPKRLSLSRTFVSSLTKIPGSNSKGVGSVPLVQGSRARNFNPVNKNLANRERGRD 215
Query: 590 TNVHLNTTKLQDLRQMIAIRENEXXXXXXXXXXXXXXXSHGTCDAMNFANDMGKQAALA- 414
V N KLQDLRQ IA+RE+E A+N N K ++
Sbjct: 216 QGVVSNDNKLQDLRQQIALRESELKLKAAQQNKESASVLGRDHSAINSKNMARKSTPVSS 275
Query: 413 -----EPKEPEKKRLKITEPRQNTMGASGQEVMPPVESMLASKNPLLENDHQQILENCCH 249
EPKEP++KRLK++ + Q+ +P V+S+L K+ LEN H Q H
Sbjct: 276 GPAQLEPKEPDRKRLKVSTSYGTSQAVDSQQEVPVVKSLLPPKDSTLENYHPQERNKIDH 335
Query: 248 HNGKSKPGTSHTIHAKGQEQNENVVPVSSKNLLVGTKEGSGTTRC-QTEKKSKLAYSSIA 72
+ + Q+Q + + S +N+ +++G G C QTEK S+L S+A
Sbjct: 336 GKKEIPLCRAEPKTITSQKQPDKHLDNSLENMPRRSRDGDGNYGCNQTEKSSRLVDPSVA 395
Query: 71 SEQNMQMANKDAETILK 21
QN AN + ++ K
Sbjct: 396 FNQNALPANMSSNSVPK 412
>ref|XP_009347444.1| PREDICTED: uncharacterized protein LOC103939114 isoform X3 [Pyrus x
bretschneideri]
Length = 1758
Score = 94.4 bits (233), Expect = 2e-16
Identities = 88/293 (30%), Positives = 127/293 (43%), Gaps = 60/293 (20%)
Frame = -3
Query: 725 QTTRAVPKVMPRKASLSRTFVSSKNKIHGATSGNRSSSGP------------DHLPKT-- 588
QT R V KVMP+K ++RTF+SS +KI GA N SGP D + K
Sbjct: 195 QTARNVDKVMPKKLGMNRTFISSMSKIRGA---NFRDSGPSSVNQGSRVRNYDSMNKNLV 251
Query: 587 ---------------------------NVHLNTTKLQDLRQMIAIRENEXXXXXXXXXXX 489
V LNT+KLQDLR IA+RE+E
Sbjct: 252 SQECGRDQGVGLNNSKLQDLRHQIRDQGVGLNTSKLQDLRHQIALRESELKLKSAQRTKE 311
Query: 488 XXXXSHGTCDAMNFANDMGKQAALA----------EPKEPEKKRLKITEPRQNTMGASGQ 339
TC N A+ A+ + EPKEP+KKRLK+ P G
Sbjct: 312 SI-----TCRDDNTASQHRDGASKSSVRYSDVTQIEPKEPDKKRLKVGGPFSTQSSVQGP 366
Query: 338 EVMPPVESMLASKNPLLENDHQQILENCCHHNGKSKPGTSHTIHAKGQEQNENVVPVSSK 159
+ +P +S+L+SK E+D H K PG + + + QN+ V +S+
Sbjct: 367 QDVPVAKSILSSKVSAAEDDGPMNRVK-VDHGQKGIPGPTELSIVEWKNQNDKHVAATSE 425
Query: 158 NLLVGTKEGSG--TTRCQTEKKSKL-----AYSSIASEQNMQMAN--KDAETI 27
N+ G K+G+G T Q+++KSKL + + S ++M N K++ET+
Sbjct: 426 NICSGVKDGAGINTKVIQSDRKSKLVDPYATLNKVTSPESMTCNNLPKNSETM 478
>ref|XP_009347443.1| PREDICTED: uncharacterized protein LOC103939114 isoform X2 [Pyrus x
bretschneideri]
Length = 1766
Score = 94.4 bits (233), Expect = 2e-16
Identities = 88/293 (30%), Positives = 127/293 (43%), Gaps = 60/293 (20%)
Frame = -3
Query: 725 QTTRAVPKVMPRKASLSRTFVSSKNKIHGATSGNRSSSGP------------DHLPKT-- 588
QT R V KVMP+K ++RTF+SS +KI GA N SGP D + K
Sbjct: 195 QTARNVDKVMPKKLGMNRTFISSMSKIRGA---NFRDSGPSSVNQGSRVRNYDSMNKNLV 251
Query: 587 ---------------------------NVHLNTTKLQDLRQMIAIRENEXXXXXXXXXXX 489
V LNT+KLQDLR IA+RE+E
Sbjct: 252 SQECGRDQGVGLNNSKLQDLRHQIRDQGVGLNTSKLQDLRHQIALRESELKLKSAQRTKE 311
Query: 488 XXXXSHGTCDAMNFANDMGKQAALA----------EPKEPEKKRLKITEPRQNTMGASGQ 339
TC N A+ A+ + EPKEP+KKRLK+ P G
Sbjct: 312 SI-----TCRDDNTASQHRDGASKSSVRYSDVTQIEPKEPDKKRLKVGGPFSTQSSVQGP 366
Query: 338 EVMPPVESMLASKNPLLENDHQQILENCCHHNGKSKPGTSHTIHAKGQEQNENVVPVSSK 159
+ +P +S+L+SK E+D H K PG + + + QN+ V +S+
Sbjct: 367 QDVPVAKSILSSKVSAAEDDGPMNRVK-VDHGQKGIPGPTELSIVEWKNQNDKHVAATSE 425
Query: 158 NLLVGTKEGSG--TTRCQTEKKSKL-----AYSSIASEQNMQMAN--KDAETI 27
N+ G K+G+G T Q+++KSKL + + S ++M N K++ET+
Sbjct: 426 NICSGVKDGAGINTKVIQSDRKSKLVDPYATLNKVTSPESMTCNNLPKNSETM 478
>ref|XP_009347442.1| PREDICTED: uncharacterized protein LOC103939114 isoform X1 [Pyrus x
bretschneideri]
Length = 1775
Score = 94.4 bits (233), Expect = 2e-16
Identities = 88/293 (30%), Positives = 127/293 (43%), Gaps = 60/293 (20%)
Frame = -3
Query: 725 QTTRAVPKVMPRKASLSRTFVSSKNKIHGATSGNRSSSGP------------DHLPKT-- 588
QT R V KVMP+K ++RTF+SS +KI GA N SGP D + K
Sbjct: 195 QTARNVDKVMPKKLGMNRTFISSMSKIRGA---NFRDSGPSSVNQGSRVRNYDSMNKNLV 251
Query: 587 ---------------------------NVHLNTTKLQDLRQMIAIRENEXXXXXXXXXXX 489
V LNT+KLQDLR IA+RE+E
Sbjct: 252 SQECGRDQGVGLNNSKLQDLRHQIRDQGVGLNTSKLQDLRHQIALRESELKLKSAQRTKE 311
Query: 488 XXXXSHGTCDAMNFANDMGKQAALA----------EPKEPEKKRLKITEPRQNTMGASGQ 339
TC N A+ A+ + EPKEP+KKRLK+ P G
Sbjct: 312 SI-----TCRDDNTASQHRDGASKSSVRYSDVTQIEPKEPDKKRLKVGGPFSTQSSVQGP 366
Query: 338 EVMPPVESMLASKNPLLENDHQQILENCCHHNGKSKPGTSHTIHAKGQEQNENVVPVSSK 159
+ +P +S+L+SK E+D H K PG + + + QN+ V +S+
Sbjct: 367 QDVPVAKSILSSKVSAAEDDGPMNRVK-VDHGQKGIPGPTELSIVEWKNQNDKHVAATSE 425
Query: 158 NLLVGTKEGSG--TTRCQTEKKSKL-----AYSSIASEQNMQMAN--KDAETI 27
N+ G K+G+G T Q+++KSKL + + S ++M N K++ET+
Sbjct: 426 NICSGVKDGAGINTKVIQSDRKSKLVDPYATLNKVTSPESMTCNNLPKNSETM 478
>ref|XP_009357694.1| PREDICTED: uncharacterized protein LOC103948392 [Pyrus x
bretschneideri]
Length = 1639
Score = 94.4 bits (233), Expect = 2e-16
Identities = 88/293 (30%), Positives = 127/293 (43%), Gaps = 60/293 (20%)
Frame = -3
Query: 725 QTTRAVPKVMPRKASLSRTFVSSKNKIHGATSGNRSSSGP------------DHLPKT-- 588
QT R V KVMP+K ++RTF+SS +KI GA N SGP D + K
Sbjct: 59 QTARNVDKVMPKKLGMNRTFISSMSKIRGA---NFRDSGPSSVNQGSRVRNYDSMNKNLV 115
Query: 587 ---------------------------NVHLNTTKLQDLRQMIAIRENEXXXXXXXXXXX 489
V LNT+KLQDLR IA+RE+E
Sbjct: 116 SQERGRDQGVGLNNSKLQDLRHQIRDQGVGLNTSKLQDLRHQIALRESELKLKSAQRTKE 175
Query: 488 XXXXSHGTCDAMNFANDMGKQAALA----------EPKEPEKKRLKITEPRQNTMGASGQ 339
TC N A+ A+ + EPKEP+KKRLK+ P G
Sbjct: 176 SI-----TCRDDNAASQHRDGASKSSVRYSDVTQIEPKEPDKKRLKVGGPFSTQSSVQGP 230
Query: 338 EVMPPVESMLASKNPLLENDHQQILENCCHHNGKSKPGTSHTIHAKGQEQNENVVPVSSK 159
+ +P +S+L+SK +E+D H K PG + + + QN+ V +S+
Sbjct: 231 QDVPVAKSILSSKVSAVEDDGPMNRVK-VDHGQKGIPGPTELSIVEWKNQNDKHVAATSE 289
Query: 158 NLLVGTKEGSG--TTRCQTEKKSKL-----AYSSIASEQNMQMAN--KDAETI 27
N+ G K+G+G T Q+++KSKL + + S ++M N K+ ET+
Sbjct: 290 NICSGVKDGAGINTKVIQSDRKSKLVDPYATLNKVTSPESMTCNNLPKNLETM 342
>ref|XP_010319262.1| PREDICTED: uncharacterized protein LOC101263805 isoform X1 [Solanum
lycopersicum]
Length = 1751
Score = 91.7 bits (226), Expect = 1e-15
Identities = 96/375 (25%), Positives = 150/375 (40%), Gaps = 30/375 (8%)
Frame = -3
Query: 1043 EKPIQAERVTSTSEDVKAGMLVPSTKSGDLVDLPTRATLHSNHHKSFEKNRGSFVPFVIX 864
E+ + + V S+D +A S+K G +D P+ S H KS +KNR FVPF+I
Sbjct: 71 ERQVHVDTVNGNSQDTQAEKNTSSSKMGRSIDAPSSTPHESAHLKSSKKNREHFVPFLIS 130
Query: 863 XXXXXXXXXXXXSKQQNALVGKGXXXXXXXXXXXXXXXXXXXXXXKQTTRAVPKVMPRKA 684
+++ + K ++ TR K+MP K
Sbjct: 131 FSDESGSDCENSGRKKIS-ASKNRTFAEEKFIKPPAPAPRRPQKLQKITRNEAKLMPSKG 189
Query: 683 SLSRTFVSSKNKIHGATSGNRSS-------------SGPDHLPKTNVHLNTTKLQDLRQM 543
++S S K +G T GN + + DH + N HLN++KL DLRQM
Sbjct: 190 AVSHNVSSLLTKPNGGTYGNAARLHCFTKFNNSNKVATLDHGKRANAHLNSSKLHDLRQM 249
Query: 542 IAIRENEXXXXXXXXXXXXXXXSHGTCDAMNFANDMG---------KQAALAEPKEPEKK 390
IAIREN+ + +C +N N L E +EP+KK
Sbjct: 250 IAIRENQ---LNLERLQNTKKLTSASCRDVNLVNKRNLVVRASRETTNDNLRELQEPDKK 306
Query: 389 RLKITEPRQNTMGASGQEVMPPVESMLASKNPLLENDHQQILENCCHHNGKSKPGTSHTI 210
R KI + ++ E+M V + SKN L++ Q L + H+ + S ++
Sbjct: 307 RKKIVSSNPSWGFSNSHEIMSMV---IGSKNCALKDSCQLELAD---HSSPGEKYLSCSV 360
Query: 209 HAKGQEQNENVVPVSSKNLLVGTKEGSGTTR--------CQTEKKSKLAYSSIASEQNMQ 54
A +Q E SS N + K+G T R E SK A +E +
Sbjct: 361 IAGQLKQKEYQGASSSTNPSLTLKDGIDTVRNLNQSSSNSSKEIASKAANKLDKTEHAAE 420
Query: 53 MANKDAETILKKSVT 9
+ ++ + +L K V+
Sbjct: 421 LGSQYNQPLLPKKVS 435
>ref|XP_004236756.1| PREDICTED: uncharacterized protein LOC101263805 isoform X2 [Solanum
lycopersicum]
Length = 1750
Score = 91.7 bits (226), Expect = 1e-15
Identities = 96/375 (25%), Positives = 150/375 (40%), Gaps = 30/375 (8%)
Frame = -3
Query: 1043 EKPIQAERVTSTSEDVKAGMLVPSTKSGDLVDLPTRATLHSNHHKSFEKNRGSFVPFVIX 864
E+ + + V S+D +A S+K G +D P+ S H KS +KNR FVPF+I
Sbjct: 71 ERQVHVDTVNGNSQDTQAEKNTSSSKMGRSIDAPSSTPHESAHLKSSKKNREHFVPFLIS 130
Query: 863 XXXXXXXXXXXXSKQQNALVGKGXXXXXXXXXXXXXXXXXXXXXXKQTTRAVPKVMPRKA 684
+++ + K ++ TR K+MP K
Sbjct: 131 FSDESGSDCENSGRKKIS-ASKNRTFAEEKFIKPPAPAPRRPQKLQKITRNEAKLMPSKG 189
Query: 683 SLSRTFVSSKNKIHGATSGNRSS-------------SGPDHLPKTNVHLNTTKLQDLRQM 543
++S S K +G T GN + + DH + N HLN++KL DLRQM
Sbjct: 190 AVSHNVSSLLTKPNGGTYGNAARLHCFTKFNNSNKVATLDHGKRANAHLNSSKLHDLRQM 249
Query: 542 IAIRENEXXXXXXXXXXXXXXXSHGTCDAMNFANDMG---------KQAALAEPKEPEKK 390
IAIREN+ + +C +N N L E +EP+KK
Sbjct: 250 IAIRENQ---LNLERLQNTKKLTSASCRDVNLVNKRNLVVRASRETTNDNLRELQEPDKK 306
Query: 389 RLKITEPRQNTMGASGQEVMPPVESMLASKNPLLENDHQQILENCCHHNGKSKPGTSHTI 210
R KI + ++ E+M V + SKN L++ Q L + H+ + S ++
Sbjct: 307 RKKIVSSNPSWGFSNSHEIMSMV---IGSKNCALKDSCQLELAD---HSSPGEKYLSCSV 360
Query: 209 HAKGQEQNENVVPVSSKNLLVGTKEGSGTTR--------CQTEKKSKLAYSSIASEQNMQ 54
A +Q E SS N + K+G T R E SK A +E +
Sbjct: 361 IAGQLKQKEYQGASSSTNPSLTLKDGIDTVRNLNQSSSNSSKEIASKAANKLDKTEHAAE 420
Query: 53 MANKDAETILKKSVT 9
+ ++ + +L K V+
Sbjct: 421 LGSQYNQPLLPKKVS 435
>ref|XP_006365712.1| PREDICTED: uncharacterized protein LOC102590636 [Solanum tuberosum]
Length = 1750
Score = 90.9 bits (224), Expect = 2e-15
Identities = 93/341 (27%), Positives = 141/341 (41%), Gaps = 25/341 (7%)
Frame = -3
Query: 1043 EKPIQAERVTSTSEDVKAGMLVPSTKSGDLVDLPTRATLHSNHHKSFEKNRGSFVPFVIX 864
E + + V S+D +A S+K G +D+P+ A S H KS +KNR FVPF+I
Sbjct: 71 EWQVHVDTVNENSQDTQAEKNTSSSKMGYSIDVPSSAPHESAHLKSSKKNREHFVPFLIS 130
Query: 863 XXXXXXXXXXXXSKQQNALVGKGXXXXXXXXXXXXXXXXXXXXXXKQTTRAVPKVMPRKA 684
+++ + K ++ TR K+MP K
Sbjct: 131 FSDESGSDCENSGRKKIS-ASKNRTLAEDKFIKPPAPAPRRPQKLQKITRNEAKLMPSKG 189
Query: 683 SLSRTFVSSKNKIHGATSGNRSS-------------SGPDHLPKTNVHLNTTKLQDLRQM 543
++S+ S K +G T GN + + DH + NVHLN++KL DLRQM
Sbjct: 190 AVSQNVSSLLTKPNGGTYGNAARLHSFTKLNNPNKVANLDHGKRANVHLNSSKLHDLRQM 249
Query: 542 IAIRENEXXXXXXXXXXXXXXXSHGTCDAMNFANDMG---------KQAALAEPKEPEKK 390
IAIREN+ + +C +N A+ L E +EP+KK
Sbjct: 250 IAIRENQ---LNLEKLQNTKKLTSASCRDVNVASKRNLVVRASRETTNDNLRELQEPDKK 306
Query: 389 RLKITEPRQNTMGASGQEVMPPVESMLASKNPLLENDHQQILENCCHHNGKSKPGTSH-- 216
R KI P + ++ E+M V + S+ L++ +Q LE H S PG +
Sbjct: 307 RQKIISPNPSWGVSNSHEIMSMV---IGSEKCALKDSNQ--LEPADH----SSPGEKYPS 357
Query: 215 -TIHAKGQEQNENVVPVSSKNLLVGTKEGSGTTRCQTEKKS 96
++ A +Q E SS N + K G T R + S
Sbjct: 358 CSVIAGQLKQKEYQGASSSTNPSLTLKNGIDTIRNLNQSSS 398
>ref|XP_008233589.1| PREDICTED: uncharacterized protein LOC103332619 [Prunus mume]
Length = 1718
Score = 90.5 bits (223), Expect = 3e-15
Identities = 72/236 (30%), Positives = 106/236 (44%), Gaps = 24/236 (10%)
Frame = -3
Query: 725 QTTRAVPKVMPRKASLSRTFVSSKNKIHGATSGNRSSSGPDHLPKTN------------- 585
QT R V KVMP+K S++RTF+SS KI G N SGP + + +
Sbjct: 178 QTARNVDKVMPKKMSMNRTFISSMTKIRGV---NSRDSGPSSVNQGSRVRNFNTMNKNVV 234
Query: 584 ---------VHLNTTKLQDLRQMIAIRENEXXXXXXXXXXXXXXXSHGTCDAMNFANDMG 432
V LN +KLQDLRQ IA+RE+E A
Sbjct: 235 SRERGYDQGVGLNNSKLQDLRQQIALRESELKLKSAQRTKESITHEASKSSARY------ 288
Query: 431 KQAALAEPKEPEKKRLKITEPRQNTMGASGQEVMPPVESMLASKNPLLENDHQQILENCC 252
EPKEP+KKR+K+ + A G + +P +S L+SK P +EN++ +
Sbjct: 289 SDVIEGEPKEPDKKRMKVGGSFSTQLSALGPQDIPVAKSTLSSKLPAVENNNPPEIVKID 348
Query: 251 HHNGKSKPGTSHTIHAKGQEQNENVVPVSSKNLLVGTKEGSG-TTRC-QTEKKSKL 90
H G++ + K + QN+ S+ + G K G+G T+C Q+ +SK+
Sbjct: 349 HGQKGIPIGSTESSIEKSKSQNDKHAAGISEKIPSGMKYGAGIDTKCIQSSGRSKM 404
>ref|XP_012568897.1| PREDICTED: uncharacterized protein LOC101514217 isoform X3 [Cicer
arietinum]
Length = 1619
Score = 86.7 bits (213), Expect = 4e-14
Identities = 80/258 (31%), Positives = 114/258 (44%), Gaps = 25/258 (9%)
Frame = -3
Query: 725 QTTRAVPKVMPRKASLSRTFVSSKNKIHGATSGNRSS----SGP--DHLPKTNVHL---- 576
Q R++ K +P+K S++RTF+SS KI + S S GP +L N L
Sbjct: 100 QNARSLHKEIPKKLSMNRTFISSVAKIPSSNSKGAGSWSLGQGPRARNLNPMNKTLASRE 159
Query: 575 -------NTTKLQDLRQMIAIRENEXXXXXXXXXXXXXXXSHGTCDAMNFANDMGKQ--- 426
N KLQDLR IA+RE+E +AMN ND G++
Sbjct: 160 RDQGALSNDNKLQDLRHQIALRESELKLKAAQQHKESALVLGKNQNAMNLKNDTGRKNIP 219
Query: 425 ----AALAEPKEPEKKRLKITEPRQNTMGASGQEVMPPVESMLASKNPLLENDHQQILEN 258
AA E KEP++KR+K+ GQ+V P V+S+L SK+ L N + Q N
Sbjct: 220 VSSGAAQLELKEPDRKRIKLNTSHDTPQAVGGQQV-PVVKSILPSKDSLCGNIYPQ-ERN 277
Query: 257 CCHHNGKSKPGTSHTIHAKGQEQNENVVPVSSKNLLVGTKEGSGTTRC-QTEKKSKLAYS 81
HN K P Q Q +N + S +N+ +EG C Q +K S+L
Sbjct: 278 KVDHNQKEIPSCKGESKVISQRQPDNHLGNSLENMPC-RREGDVNYGCYQADKSSRLVDP 336
Query: 80 SIASEQNMQMANKDAETI 27
A Q+ AN + ++
Sbjct: 337 CAAFNQSSVPANMPSNSV 354
>ref|XP_012568896.1| PREDICTED: uncharacterized protein LOC101514217 isoform X2 [Cicer
arietinum]
Length = 1675
Score = 86.7 bits (213), Expect = 4e-14
Identities = 80/258 (31%), Positives = 114/258 (44%), Gaps = 25/258 (9%)
Frame = -3
Query: 725 QTTRAVPKVMPRKASLSRTFVSSKNKIHGATSGNRSS----SGP--DHLPKTNVHL---- 576
Q R++ K +P+K S++RTF+SS KI + S S GP +L N L
Sbjct: 156 QNARSLHKEIPKKLSMNRTFISSVAKIPSSNSKGAGSWSLGQGPRARNLNPMNKTLASRE 215
Query: 575 -------NTTKLQDLRQMIAIRENEXXXXXXXXXXXXXXXSHGTCDAMNFANDMGKQ--- 426
N KLQDLR IA+RE+E +AMN ND G++
Sbjct: 216 RDQGALSNDNKLQDLRHQIALRESELKLKAAQQHKESALVLGKNQNAMNLKNDTGRKNIP 275
Query: 425 ----AALAEPKEPEKKRLKITEPRQNTMGASGQEVMPPVESMLASKNPLLENDHQQILEN 258
AA E KEP++KR+K+ GQ+V P V+S+L SK+ L N + Q N
Sbjct: 276 VSSGAAQLELKEPDRKRIKLNTSHDTPQAVGGQQV-PVVKSILPSKDSLCGNIYPQ-ERN 333
Query: 257 CCHHNGKSKPGTSHTIHAKGQEQNENVVPVSSKNLLVGTKEGSGTTRC-QTEKKSKLAYS 81
HN K P Q Q +N + S +N+ +EG C Q +K S+L
Sbjct: 334 KVDHNQKEIPSCKGESKVISQRQPDNHLGNSLENMPC-RREGDVNYGCYQADKSSRLVDP 392
Query: 80 SIASEQNMQMANKDAETI 27
A Q+ AN + ++
Sbjct: 393 CAAFNQSSVPANMPSNSV 410
>ref|XP_004492099.1| PREDICTED: uncharacterized protein LOC101514217 isoform X1 [Cicer
arietinum]
Length = 1676
Score = 86.7 bits (213), Expect = 4e-14
Identities = 80/258 (31%), Positives = 114/258 (44%), Gaps = 25/258 (9%)
Frame = -3
Query: 725 QTTRAVPKVMPRKASLSRTFVSSKNKIHGATSGNRSS----SGP--DHLPKTNVHL---- 576
Q R++ K +P+K S++RTF+SS KI + S S GP +L N L
Sbjct: 157 QNARSLHKEIPKKLSMNRTFISSVAKIPSSNSKGAGSWSLGQGPRARNLNPMNKTLASRE 216
Query: 575 -------NTTKLQDLRQMIAIRENEXXXXXXXXXXXXXXXSHGTCDAMNFANDMGKQ--- 426
N KLQDLR IA+RE+E +AMN ND G++
Sbjct: 217 RDQGALSNDNKLQDLRHQIALRESELKLKAAQQHKESALVLGKNQNAMNLKNDTGRKNIP 276
Query: 425 ----AALAEPKEPEKKRLKITEPRQNTMGASGQEVMPPVESMLASKNPLLENDHQQILEN 258
AA E KEP++KR+K+ GQ+V P V+S+L SK+ L N + Q N
Sbjct: 277 VSSGAAQLELKEPDRKRIKLNTSHDTPQAVGGQQV-PVVKSILPSKDSLCGNIYPQ-ERN 334
Query: 257 CCHHNGKSKPGTSHTIHAKGQEQNENVVPVSSKNLLVGTKEGSGTTRC-QTEKKSKLAYS 81
HN K P Q Q +N + S +N+ +EG C Q +K S+L
Sbjct: 335 KVDHNQKEIPSCKGESKVISQRQPDNHLGNSLENMPC-RREGDVNYGCYQADKSSRLVDP 393
Query: 80 SIASEQNMQMANKDAETI 27
A Q+ AN + ++
Sbjct: 394 CAAFNQSSVPANMPSNSV 411
>ref|XP_009600592.1| PREDICTED: uncharacterized protein LOC104096018 isoform X2 [Nicotiana
tomentosiformis]
Length = 1715
Score = 84.0 bits (206), Expect = 2e-13
Identities = 96/375 (25%), Positives = 152/375 (40%), Gaps = 30/375 (8%)
Frame = -3
Query: 1043 EKPIQAERVTSTSEDVKAGMLVPSTKSGDLVDLPTRATLHSNHHKSFEKNRGSFVPFVIX 864
E+ + + V S+D +A S+K G +D+P+ S+H KS +KNR FVPF+I
Sbjct: 70 EQQVHIDTVNENSQDTQAEKCTSSSKMGCSIDVPSGTPPESDHLKSSKKNREHFVPFLIS 129
Query: 863 XXXXXXXXXXXXSKQQNALVGKGXXXXXXXXXXXXXXXXXXXXXXKQTTRAVPKVMPRKA 684
Q+ V K + TR P +M RK
Sbjct: 130 FSDDSGSECENSG-QKKISVSKNRTLAADRSNKPPAPAPRRSHKLHKITRKDPNLMSRKG 188
Query: 683 SLSRTFVSSKNKIHGATSGN-------RSSSGP------DHLPKTNVHLNTTKLQDLRQM 543
SS KI G T GN R+ + DH +TNVH N+++L DLR +
Sbjct: 189 P-----ASSFTKIKGCTYGNVGHLHSMRNFNNSNRVAILDHRKRTNVHSNSSELHDLRHL 243
Query: 542 IAIRENEXXXXXXXXXXXXXXXSHGTCDAMNFAND------MGKQAA---LAEPKEPEKK 390
IAIRE++ + +C N N K+ L E KEP+ K
Sbjct: 244 IAIRESQ---LNLKRSQNTKNLTSASCRDRNLVNKRNLVVRASKETTYDYLQELKEPDNK 300
Query: 389 RLKITEPRQNTMGASGQEVMPPVESMLASKNPLLENDHQQILENCCHHNGKSKPGTSHTI 210
R KI + ++ QE+ V + + + L ++D + ++ H G+ P S +
Sbjct: 301 RQKIVLEDPSWGFSNSQEITSMV--IRSERCALKDSDQLERADDSVH--GEKYPTCS--V 354
Query: 209 HAKGQEQNENVVPVSSKNLLVGTKEGSGTTR--------CQTEKKSKLAYSSIASEQNMQ 54
A +QNE SS N + K+G R E SK A + ++ +
Sbjct: 355 LAGQLKQNEYHGSASSANPSLTLKDGIDAVRNHNQSFNNSSKEVASKAANKLVKTKHATE 414
Query: 53 MANKDAETILKKSVT 9
++++ + +L+K VT
Sbjct: 415 LSSQCRQPLLQKKVT 429
>ref|XP_009600591.1| PREDICTED: uncharacterized protein LOC104096018 isoform X1 [Nicotiana
tomentosiformis]
Length = 1750
Score = 84.0 bits (206), Expect = 2e-13
Identities = 96/375 (25%), Positives = 152/375 (40%), Gaps = 30/375 (8%)
Frame = -3
Query: 1043 EKPIQAERVTSTSEDVKAGMLVPSTKSGDLVDLPTRATLHSNHHKSFEKNRGSFVPFVIX 864
E+ + + V S+D +A S+K G +D+P+ S+H KS +KNR FVPF+I
Sbjct: 70 EQQVHIDTVNENSQDTQAEKCTSSSKMGCSIDVPSGTPPESDHLKSSKKNREHFVPFLIS 129
Query: 863 XXXXXXXXXXXXSKQQNALVGKGXXXXXXXXXXXXXXXXXXXXXXKQTTRAVPKVMPRKA 684
Q+ V K + TR P +M RK
Sbjct: 130 FSDDSGSECENSG-QKKISVSKNRTLAADRSNKPPAPAPRRSHKLHKITRKDPNLMSRKG 188
Query: 683 SLSRTFVSSKNKIHGATSGN-------RSSSGP------DHLPKTNVHLNTTKLQDLRQM 543
SS KI G T GN R+ + DH +TNVH N+++L DLR +
Sbjct: 189 P-----ASSFTKIKGCTYGNVGHLHSMRNFNNSNRVAILDHRKRTNVHSNSSELHDLRHL 243
Query: 542 IAIRENEXXXXXXXXXXXXXXXSHGTCDAMNFAND------MGKQAA---LAEPKEPEKK 390
IAIRE++ + +C N N K+ L E KEP+ K
Sbjct: 244 IAIRESQ---LNLKRSQNTKNLTSASCRDRNLVNKRNLVVRASKETTYDYLQELKEPDNK 300
Query: 389 RLKITEPRQNTMGASGQEVMPPVESMLASKNPLLENDHQQILENCCHHNGKSKPGTSHTI 210
R KI + ++ QE+ V + + + L ++D + ++ H G+ P S +
Sbjct: 301 RQKIVLEDPSWGFSNSQEITSMV--IRSERCALKDSDQLERADDSVH--GEKYPTCS--V 354
Query: 209 HAKGQEQNENVVPVSSKNLLVGTKEGSGTTR--------CQTEKKSKLAYSSIASEQNMQ 54
A +QNE SS N + K+G R E SK A + ++ +
Sbjct: 355 LAGQLKQNEYHGSASSANPSLTLKDGIDAVRNHNQSFNNSSKEVASKAANKLVKTKHATE 414
Query: 53 MANKDAETILKKSVT 9
++++ + +L+K VT
Sbjct: 415 LSSQCRQPLLQKKVT 429
>ref|XP_009798390.1| PREDICTED: uncharacterized protein LOC104244624 isoform X2 [Nicotiana
sylvestris]
Length = 1707
Score = 82.8 bits (203), Expect = 5e-13
Identities = 96/375 (25%), Positives = 145/375 (38%), Gaps = 30/375 (8%)
Frame = -3
Query: 1043 EKPIQAERVTSTSEDVKAGMLVPSTKSGDLVDLPTRATLHSNHHKSFEKNRGSFVPFVIX 864
E+ + + V S+D +A S+K G +D+P+ S+H KS +KNR FVPF+I
Sbjct: 71 EQQVHIDTVNENSQDTQAEKCTSSSKMGCSIDVPSGTLPESDHPKSSKKNREHFVPFLIS 130
Query: 863 XXXXXXXXXXXXSKQQNALVGKGXXXXXXXXXXXXXXXXXXXXXXKQTTRAVPKVMPRKA 684
Q+ V K + TR +M RK
Sbjct: 131 FSDDSGSECENSG-QKKISVSKNRTSAADRSNKPPAPASRRSHKLHKITRKDANLMSRKG 189
Query: 683 SLSRTFVSSKNKIHGATSGN-------RSSSGP------DHLPKTNVHLNTTKLQDLRQM 543
SS KI G T GN R+ + DH KTNVH N+++L DLR +
Sbjct: 190 P-----ASSFTKIKGGTYGNVGHLHSMRNFNNSNKVAILDHRKKTNVHSNSSELHDLRHL 244
Query: 542 IAIRENEXXXXXXXXXXXXXXXSHGTCDAMNFAND------MGKQAA---LAEPKEPEKK 390
IAIRE++ + +C N N K+ L E KEP+ K
Sbjct: 245 IAIRESQ---LNLKRSQNTTNLTSASCRDRNLVNKRNLVVRASKETTYDYLQELKEPDNK 301
Query: 389 RLKITEPRQNTMGASGQEVMPPVESMLASKNPLLENDHQQILENCCHHNGKSKPGTSHTI 210
R KI + ++ QE + SM+ D Q+ +G+ P S +
Sbjct: 302 RQKIVSENPSWGFSNSQE----ITSMVIRSEMCALKDSDQLERADDSSHGEKYPTCS--V 355
Query: 209 HAKGQEQNENVVPVSSKNLLVGTKEGSGTTR--------CQTEKKSKLAYSSIASEQNMQ 54
A +QNE SS N + K+G R E SK A + ++ +
Sbjct: 356 LAGQLKQNEYQGCASSANPPLSLKDGIDAVRNHNQNFNNSSKEVASKAANKLVKTKHASE 415
Query: 53 MANKDAETILKKSVT 9
++ + + +L+K VT
Sbjct: 416 LSGQCRQPLLQKKVT 430
>ref|XP_009798389.1| PREDICTED: uncharacterized protein LOC104244624 isoform X1 [Nicotiana
sylvestris]
Length = 1742
Score = 82.8 bits (203), Expect = 5e-13
Identities = 96/375 (25%), Positives = 145/375 (38%), Gaps = 30/375 (8%)
Frame = -3
Query: 1043 EKPIQAERVTSTSEDVKAGMLVPSTKSGDLVDLPTRATLHSNHHKSFEKNRGSFVPFVIX 864
E+ + + V S+D +A S+K G +D+P+ S+H KS +KNR FVPF+I
Sbjct: 71 EQQVHIDTVNENSQDTQAEKCTSSSKMGCSIDVPSGTLPESDHPKSSKKNREHFVPFLIS 130
Query: 863 XXXXXXXXXXXXSKQQNALVGKGXXXXXXXXXXXXXXXXXXXXXXKQTTRAVPKVMPRKA 684
Q+ V K + TR +M RK
Sbjct: 131 FSDDSGSECENSG-QKKISVSKNRTSAADRSNKPPAPASRRSHKLHKITRKDANLMSRKG 189
Query: 683 SLSRTFVSSKNKIHGATSGN-------RSSSGP------DHLPKTNVHLNTTKLQDLRQM 543
SS KI G T GN R+ + DH KTNVH N+++L DLR +
Sbjct: 190 P-----ASSFTKIKGGTYGNVGHLHSMRNFNNSNKVAILDHRKKTNVHSNSSELHDLRHL 244
Query: 542 IAIRENEXXXXXXXXXXXXXXXSHGTCDAMNFAND------MGKQAA---LAEPKEPEKK 390
IAIRE++ + +C N N K+ L E KEP+ K
Sbjct: 245 IAIRESQ---LNLKRSQNTTNLTSASCRDRNLVNKRNLVVRASKETTYDYLQELKEPDNK 301
Query: 389 RLKITEPRQNTMGASGQEVMPPVESMLASKNPLLENDHQQILENCCHHNGKSKPGTSHTI 210
R KI + ++ QE + SM+ D Q+ +G+ P S +
Sbjct: 302 RQKIVSENPSWGFSNSQE----ITSMVIRSEMCALKDSDQLERADDSSHGEKYPTCS--V 355
Query: 209 HAKGQEQNENVVPVSSKNLLVGTKEGSGTTR--------CQTEKKSKLAYSSIASEQNMQ 54
A +QNE SS N + K+G R E SK A + ++ +
Sbjct: 356 LAGQLKQNEYQGCASSANPPLSLKDGIDAVRNHNQNFNNSSKEVASKAANKLVKTKHASE 415
Query: 53 MANKDAETILKKSVT 9
++ + + +L+K VT
Sbjct: 416 LSGQCRQPLLQKKVT 430
>ref|XP_010658598.1| PREDICTED: uncharacterized protein LOC100253034 [Vitis vinifera]
Length = 1746
Score = 81.6 bits (200), Expect = 1e-12
Identities = 96/410 (23%), Positives = 159/410 (38%), Gaps = 55/410 (13%)
Frame = -3
Query: 1079 SASPFIKCNALQEKPIQAERVTSTSEDVKAGMLVPSTKSGDLVDLPTRATLHSNHHKSFE 900
S +P + +PI ++ KAG V + +D+ R ++ N+HK FE
Sbjct: 50 SPAPSVGATVPPVEPILVAPSNKNTQGTKAGKSVSVNNAAISIDIQARTSIQPNYHKGFE 109
Query: 899 KNR--------------GSFVPFVIXXXXXXXXXXXXXSKQQNA--LVGKGXXXXXXXXX 768
KNR GS VI Q+ A L KG
Sbjct: 110 KNRVPFKSGSSGWYGPPGSNNNLVISFSDNDSGSDSEEYGQEKASTLETKGDTVRVDGNK 169
Query: 767 XXXXXXXXXXXXXKQTTRAVPKVMPRKASLSRTFVSSK------------------NKIH 642
++TT K++P+K LSR F+ S +++
Sbjct: 170 RTPASSVRKSEMLERTTGTETKMVPKKVPLSRRFIQSTKAKGFNSRNAGPLLIEQGSRVG 229
Query: 641 GATSGNRSSSGPDHLPKTNVHLNTTKLQDLRQMIAIRENEXXXXXXXXXXXXXXXSH--- 471
++ N++ + D V LN +KLQDLRQ IA+RE+E +
Sbjct: 230 NFSALNKNLAKRDREVTQGVFLNNSKLQDLRQQIALRESELKLKSAQQNKEIVSQQNKET 289
Query: 470 --GTC---DAMNFAND-MGKQAALA------EPKEPEKKRLKI--TEPRQNTMGASGQEV 333
G+C ++MN N GK + + EPKEP+ KRLK+ T RQ +
Sbjct: 290 VSGSCKDNNSMNLNNSTTGKSRSTSIDIQQLEPKEPDGKRLKVSGTYSRQINSNLDDRHD 349
Query: 332 MPPVESMLASKNPLLEN----DHQQILENCCHHNGKSKPGTSHTIHAKGQEQNENVVPVS 165
+P +S+L K P ++ D +I + C + S + K ++Q+E VS
Sbjct: 350 VPAAKSLLGLKEPASQSSGLLDRDKIDHSYCEKEVPANRTQSSIV--KWKKQDEKRPAVS 407
Query: 164 SKNLLVGTKEGSGTTRCQTEKKSKLAYSSIASEQNMQMANKDAETILKKS 15
+NL + G + Q+++ ++ + Q + +AN + K+S
Sbjct: 408 LENLRKNGADNIGDS--QSDRNARQVDRLVVLNQTVPLANMASNASPKRS 455
>ref|XP_007009394.1| Zinc finger C3H1 domain-containing protein, putative [Theobroma
cacao] gi|508726307|gb|EOY18204.1| Zinc finger C3H1
domain-containing protein, putative [Theobroma cacao]
Length = 1749
Score = 79.7 bits (195), Expect = 4e-12
Identities = 91/372 (24%), Positives = 145/372 (38%), Gaps = 43/372 (11%)
Frame = -3
Query: 1037 PIQAERVTSTSEDVKAGMLVPSTKSGDLVDLPTRATLHSNHHKSFEKNR----------- 891
P A + AG V VD+P+R ++ K+ EKNR
Sbjct: 62 PTSAPSAVKSPPPNLAGNTVSGNNLASTVDIPSRQSVPPKSQKNIEKNRLPFESSNPSWY 121
Query: 890 ---GSFVPFVIXXXXXXXXXXXXXSKQQNALVGKGXXXXXXXXXXXXXXXXXXXXXXKQT 720
G VI QQ + K QT
Sbjct: 122 APSGGSNNLVIRFTDDESGSDSEECSQQRTVEHKANSTTDGCRRPVTSSAPKSNKLG-QT 180
Query: 719 TRAVPKVMPRKASLSRTFVSSKNKIHGATSG--------------------NRSSSGPDH 600
+R + +V+P+K LSRTF SS KI+G + N++ + D
Sbjct: 181 SRNITRVIPKKP-LSRTFSSSMTKINGGANSRVAGPSAVDQGSRIRYLNPRNKNMASQDL 239
Query: 599 LPKTNVHLNTTKLQDLRQMIAIRENEXXXXXXXXXXXXXXXSHGTCDAMNFANDMGKQ-- 426
V LN +KLQDLRQ IA+RE+E + +N N G++
Sbjct: 240 GYDLGVGLNNSKLQDLRQQIALRESELKLKAAQQNKEAV-----SASTLNLDNGAGRKWT 294
Query: 425 -----AALAEPKEPEKKRLKITEPRQNTMGASGQEVMPPVESMLASKNPLLENDHQQILE 261
A +PKEP+KKRLKI+E + + Q+ + ++S L SK+ LE + + +
Sbjct: 295 PTSVDAGPLDPKEPDKKRLKISESSFTHLNSDAQQEVHLLKSNLVSKDQQLETNSLRSRD 354
Query: 260 NCCHHNGKSKPGTSHTIHAKGQEQNENVVPVSSKNLLVGTKEG--SGTTRCQTEKKSKLA 87
H+ K P + + K Q++++ +V VSS + K+G T Q+++ S+
Sbjct: 355 K-VDHSKKVVPSKAKS-SIKWQKKDDKLVDVSSDDTSKVVKDGVDPQTNLHQSKRTSRQV 412
Query: 86 YSSIASEQNMQM 51
S+ + Q +
Sbjct: 413 DLSVLANQTASL 424