BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Forsythia23_contig00019952
         (347 letters)

Database: ./nr 
           69,698,275 sequences; 24,982,196,650 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011089586.1| PREDICTED: probably inactive leucine-rich re...   100   4e-19
ref|XP_002527290.1| ATP binding protein, putative [Ricinus commu...    89   9e-16
ref|XP_010251401.1| PREDICTED: probably inactive leucine-rich re...    86   7e-15
ref|XP_009358936.1| PREDICTED: probably inactive leucine-rich re...    85   2e-14
ref|XP_008372669.1| PREDICTED: probably inactive leucine-rich re...    85   2e-14
ref|XP_010242455.1| PREDICTED: probably inactive leucine-rich re...    84   3e-14
ref|XP_012833589.1| PREDICTED: probably inactive leucine-rich re...    84   4e-14
ref|XP_008356275.1| PREDICTED: probably inactive leucine-rich re...    84   4e-14
ref|XP_010109951.1| Probably inactive leucine-rich repeat recept...    84   5e-14
ref|XP_012490947.1| PREDICTED: probably inactive leucine-rich re...    83   6e-14
ref|XP_004510191.1| PREDICTED: probably inactive leucine-rich re...    83   6e-14
ref|XP_009595674.1| PREDICTED: probably inactive leucine-rich re...    83   8e-14
ref|XP_004148961.2| PREDICTED: probably inactive leucine-rich re...    82   1e-13
ref|XP_009799665.1| PREDICTED: probably inactive leucine-rich re...    82   1e-13
ref|XP_010101366.1| Probably inactive leucine-rich repeat recept...    82   1e-13
emb|CDP03993.1| unnamed protein product [Coffea canephora]             82   2e-13
ref|XP_007049531.1| Probably inactive leucine-rich repeat recept...    82   2e-13
ref|XP_007200186.1| hypothetical protein PRUPE_ppa016209mg [Prun...    82   2e-13
ref|XP_010095584.1| Probably inactive leucine-rich repeat recept...    81   2e-13
ref|XP_007200419.1| hypothetical protein PRUPE_ppa011049mg [Prun...    81   3e-13

>ref|XP_011089586.1| PREDICTED: probably inactive leucine-rich repeat receptor-like
           protein kinase At5g48380 [Sesamum indicum]
           gi|747084363|ref|XP_011089587.1| PREDICTED: probably
           inactive leucine-rich repeat receptor-like protein
           kinase At5g48380 [Sesamum indicum]
          Length = 620

 Score =  100 bits (249), Expect = 4e-19
 Identities = 43/83 (51%), Positives = 61/83 (73%), Gaps = 1/83 (1%)
 Frame = -2

Query: 247 MAAFFMRIVLNYRALPIL-AVFIWSLFSESFVYAAQNDVDCLRVIRSSLKDPNGIFTTWD 71
           M A  M ++L +RALP L ++FIW L  ESF +AAQ D+DCLR I+ SL+DP    ++W+
Sbjct: 1   MIAVVMSVLLGFRALPALVSIFIWLLVIESFGHAAQTDIDCLRAIKISLEDPFNYLSSWN 60

Query: 70  FNNKSNGFICTFIGIDCWHPDEN 2
           F+N + G+IC F G++CWHP+EN
Sbjct: 61  FDNTTEGYICKFTGVECWHPNEN 83


>ref|XP_002527290.1| ATP binding protein, putative [Ricinus communis]
           gi|223533383|gb|EEF35134.1| ATP binding protein,
           putative [Ricinus communis]
          Length = 302

 Score = 89.4 bits (220), Expect = 9e-16
 Identities = 37/81 (45%), Positives = 61/81 (75%), Gaps = 2/81 (2%)
 Frame = -2

Query: 238 FFMRIVLNYR-ALPILAVFIWSLFSESFVYAAQNDVDCLRVIRSSLKDP-NGIFTTWDFN 65
           FF++++L  R ++ +++  +W L S S  YA + D+ CL+ I++SL+DP N + ++WDFN
Sbjct: 3   FFVQMMLGRRVSMLLISSSLWLLLSCSLSYATETDIACLKSIKASLEDPLNNLNSSWDFN 62

Query: 64  NKSNGFICTFIGIDCWHPDEN 2
           NK+ G+IC F+G++CWHPDEN
Sbjct: 63  NKTEGYICKFVGVECWHPDEN 83


>ref|XP_010251401.1| PREDICTED: probably inactive leucine-rich repeat receptor-like
           protein kinase At5g48380 [Nelumbo nucifera]
           gi|719985484|ref|XP_010251402.1| PREDICTED: probably
           inactive leucine-rich repeat receptor-like protein
           kinase At5g48380 [Nelumbo nucifera]
           gi|719985487|ref|XP_010251403.1| PREDICTED: probably
           inactive leucine-rich repeat receptor-like protein
           kinase At5g48380 [Nelumbo nucifera]
          Length = 617

 Score = 86.3 bits (212), Expect = 7e-15
 Identities = 34/73 (46%), Positives = 50/73 (68%)
 Frame = -2

Query: 220 LNYRALPILAVFIWSLFSESFVYAAQNDVDCLRVIRSSLKDPNGIFTTWDFNNKSNGFIC 41
           +NY  L +L+   W L + +    +  D++CL+ ++SSL+DP    ++W+FNNK+ GFIC
Sbjct: 5   MNYSCLAVLSAISWLLLTATKSDGSDADINCLKSLKSSLEDPFNYLSSWNFNNKTEGFIC 64

Query: 40  TFIGIDCWHPDEN 2
            F GIDCWHPDEN
Sbjct: 65  KFTGIDCWHPDEN 77


>ref|XP_009358936.1| PREDICTED: probably inactive leucine-rich repeat receptor-like
           protein kinase At5g48380 [Pyrus x bretschneideri]
          Length = 626

 Score = 84.7 bits (208), Expect = 2e-14
 Identities = 39/82 (47%), Positives = 58/82 (70%), Gaps = 2/82 (2%)
 Frame = -2

Query: 241 AFFMRIVLNYRALPIL-AVFIWSLFSESFVYAAQNDVDCLRVIRSSLKDPNGIF-TTWDF 68
           AF M ++LN R   I     +W L S SF +A ++D++CL+ +++SL+DP G   ++WDF
Sbjct: 2   AFSMYMLLNGRDPGIFFGGVVWLLLSCSFSFAVESDINCLKSLKASLQDPLGYLNSSWDF 61

Query: 67  NNKSNGFICTFIGIDCWHPDEN 2
           NNK+ GFIC F+GI+CWHP E+
Sbjct: 62  NNKTEGFICNFLGIECWHPHES 83


>ref|XP_008372669.1| PREDICTED: probably inactive leucine-rich repeat receptor-like
           protein kinase At5g48380 [Malus domestica]
          Length = 626

 Score = 84.7 bits (208), Expect = 2e-14
 Identities = 39/82 (47%), Positives = 58/82 (70%), Gaps = 2/82 (2%)
 Frame = -2

Query: 241 AFFMRIVLNYRALPIL-AVFIWSLFSESFVYAAQNDVDCLRVIRSSLKDPNGIF-TTWDF 68
           AF M ++LN R   I     +W L S SF +A ++D++CL+ +++SL+DP G   ++WDF
Sbjct: 2   AFSMYMMLNGRDPGIFFGGVVWLLLSCSFSFAVESDINCLKSLKASLQDPLGYLNSSWDF 61

Query: 67  NNKSNGFICTFIGIDCWHPDEN 2
           NNK+ GFIC F+GI+CWHP E+
Sbjct: 62  NNKTEGFICNFLGIECWHPHES 83


>ref|XP_010242455.1| PREDICTED: probably inactive leucine-rich repeat receptor-like
           protein kinase At5g48380 [Nelumbo nucifera]
          Length = 630

 Score = 84.3 bits (207), Expect = 3e-14
 Identities = 36/80 (45%), Positives = 52/80 (65%), Gaps = 2/80 (2%)
 Frame = -2

Query: 235 FMRIVLNYRALPILA-VFIWSLFSESFVYAAQNDVDCLRVIRSSLKDPNGIF-TTWDFNN 62
           ++ + +N   L +L   F W L S S ++  + D+ CL+ ++ SLKDP G   T+W+F N
Sbjct: 4   YVLMTMNINVLAVLCFAFFWLLLSASVIHGTETDIYCLKTLKDSLKDPFGYLNTSWNFAN 63

Query: 61  KSNGFICTFIGIDCWHPDEN 2
           K+ GFIC F GIDCWHPDE+
Sbjct: 64  KTEGFICKFTGIDCWHPDES 83


>ref|XP_012833589.1| PREDICTED: probably inactive leucine-rich repeat receptor-like
           protein kinase At5g48380 [Erythranthe guttatus]
          Length = 621

 Score = 84.0 bits (206), Expect = 4e-14
 Identities = 37/77 (48%), Positives = 54/77 (70%), Gaps = 2/77 (2%)
 Frame = -2

Query: 226 IVLNYRA-LPILAVFIWSLFSESFV-YAAQNDVDCLRVIRSSLKDPNGIFTTWDFNNKSN 53
           +V NYR    ++A+ IW L +ESF   A+Q D+DCL+ +++SLKDP+    +W+FNN   
Sbjct: 1   MVSNYRPPYALIAIVIWFLSTESFFSQASQADIDCLKSVKASLKDPSNYLASWNFNNTVE 60

Query: 52  GFICTFIGIDCWHPDEN 2
           G+IC F GI+CWH +EN
Sbjct: 61  GYICKFTGIECWHENEN 77


>ref|XP_008356275.1| PREDICTED: probably inactive leucine-rich repeat receptor-like
           protein kinase At5g48380 [Malus domestica]
          Length = 626

 Score = 84.0 bits (206), Expect = 4e-14
 Identities = 39/82 (47%), Positives = 57/82 (69%), Gaps = 2/82 (2%)
 Frame = -2

Query: 241 AFFMRIVLNYRALPIL-AVFIWSLFSESFVYAAQNDVDCLRVIRSSLKDPNGIF-TTWDF 68
           AF M + LN R   I     +W L S SF +A ++D++CL+ +++SL+DP G   ++WDF
Sbjct: 2   AFSMYMXLNGRDPGIFFGGVVWLLLSCSFSFAVESDINCLKSLKASLQDPLGYLNSSWDF 61

Query: 67  NNKSNGFICTFIGIDCWHPDEN 2
           NNK+ GFIC F+GI+CWHP E+
Sbjct: 62  NNKTEGFICNFLGIECWHPHES 83


>ref|XP_010109951.1| Probably inactive leucine-rich repeat receptor-like protein kinase
           [Morus notabilis] gi|587938160|gb|EXC24927.1| Probably
           inactive leucine-rich repeat receptor-like protein
           kinase [Morus notabilis]
          Length = 280

 Score = 83.6 bits (205), Expect = 5e-14
 Identities = 34/63 (53%), Positives = 43/63 (68%)
 Frame = -2

Query: 190 VFIWSLFSESFVYAAQNDVDCLRVIRSSLKDPNGIFTTWDFNNKSNGFICTFIGIDCWHP 11
           V + S+ S S  +A  ND+ CL+ I+ S  DP G   +WDFNN + GFIC FIG+DCWHP
Sbjct: 10  VVLLSISSISLSHATLNDIYCLKTIKKSFNDPYGYLASWDFNNNTEGFICKFIGVDCWHP 69

Query: 10  DEN 2
           DEN
Sbjct: 70  DEN 72


>ref|XP_012490947.1| PREDICTED: probably inactive leucine-rich repeat receptor-like
           protein kinase At5g48380 [Gossypium raimondii]
           gi|823189795|ref|XP_012490948.1| PREDICTED: probably
           inactive leucine-rich repeat receptor-like protein
           kinase At5g48380 [Gossypium raimondii]
           gi|823189798|ref|XP_012490949.1| PREDICTED: probably
           inactive leucine-rich repeat receptor-like protein
           kinase At5g48380 [Gossypium raimondii]
           gi|823189801|ref|XP_012490950.1| PREDICTED: probably
           inactive leucine-rich repeat receptor-like protein
           kinase At5g48380 [Gossypium raimondii]
           gi|763775515|gb|KJB42638.1| hypothetical protein
           B456_007G161400 [Gossypium raimondii]
           gi|763775516|gb|KJB42639.1| hypothetical protein
           B456_007G161400 [Gossypium raimondii]
           gi|763775517|gb|KJB42640.1| hypothetical protein
           B456_007G161400 [Gossypium raimondii]
           gi|763775518|gb|KJB42641.1| hypothetical protein
           B456_007G161400 [Gossypium raimondii]
          Length = 621

 Score = 83.2 bits (204), Expect = 6e-14
 Identities = 35/67 (52%), Positives = 48/67 (71%), Gaps = 1/67 (1%)
 Frame = -2

Query: 199 ILAVFIWSLFSESFVYAAQNDVDCLRVIRSSLKDPNGIF-TTWDFNNKSNGFICTFIGID 23
           +++V +W L   S  YA+Q D+DCLR I+ SL+D  G   ++W+F+N + GFIC F GID
Sbjct: 13  LVSVLVWLLLGCSLSYASQEDIDCLRSIKDSLEDSLGYLNSSWNFDNTTEGFICRFTGID 72

Query: 22  CWHPDEN 2
           CWHPDEN
Sbjct: 73  CWHPDEN 79


>ref|XP_004510191.1| PREDICTED: probably inactive leucine-rich repeat receptor-like
           protein kinase At5g48380 isoform X1 [Cicer arietinum]
           gi|502155786|ref|XP_004510192.1| PREDICTED: probably
           inactive leucine-rich repeat receptor-like protein
           kinase At5g48380 isoform X1 [Cicer arietinum]
           gi|502155789|ref|XP_004510193.1| PREDICTED: probably
           inactive leucine-rich repeat receptor-like protein
           kinase At5g48380 isoform X1 [Cicer arietinum]
           gi|502155792|ref|XP_004510194.1| PREDICTED: probably
           inactive leucine-rich repeat receptor-like protein
           kinase At5g48380 isoform X2 [Cicer arietinum]
          Length = 618

 Score = 83.2 bits (204), Expect = 6e-14
 Identities = 34/63 (53%), Positives = 44/63 (69%), Gaps = 1/63 (1%)
 Frame = -2

Query: 187 FIWSLFSESFVYAAQNDVDCLRVIRSSLKDPNGIF-TTWDFNNKSNGFICTFIGIDCWHP 11
           F+  + S    Y  + D+ CL+ I+ SLKDPNG   T+WDFNNK+ GFIC F G++CWHP
Sbjct: 17  FLLLIISWGITYGTETDILCLKSIKESLKDPNGYLKTSWDFNNKTEGFICRFTGVECWHP 76

Query: 10  DEN 2
           DEN
Sbjct: 77  DEN 79


>ref|XP_009595674.1| PREDICTED: probably inactive leucine-rich repeat receptor-like
           protein kinase At5g48380 [Nicotiana tomentosiformis]
           gi|697173501|ref|XP_009595675.1| PREDICTED: probably
           inactive leucine-rich repeat receptor-like protein
           kinase At5g48380 [Nicotiana tomentosiformis]
          Length = 626

 Score = 82.8 bits (203), Expect = 8e-14
 Identities = 41/80 (51%), Positives = 56/80 (70%), Gaps = 2/80 (2%)
 Frame = -2

Query: 235 FMRIVLNYRALPI-LAVFIWSLFSESFVYAAQNDVDCLRVIRSSLKDPNGIF-TTWDFNN 62
           F ++V N  AL I  A  I+ L S +   A Q+D+DCL+ IRSSL+D + I  ++W+FNN
Sbjct: 5   FKKMVSNCGALTIPAATLIYLLLSCTVCCALQSDIDCLKSIRSSLEDTSNILASSWNFNN 64

Query: 61  KSNGFICTFIGIDCWHPDEN 2
           ++ GFIC F GI+CWHPDEN
Sbjct: 65  QTEGFICKFTGIECWHPDEN 84


>ref|XP_004148961.2| PREDICTED: probably inactive leucine-rich repeat receptor-like
           protein kinase At5g48380 [Cucumis sativus]
           gi|778727273|ref|XP_011659235.1| PREDICTED: probably
           inactive leucine-rich repeat receptor-like protein
           kinase At5g48380 [Cucumis sativus]
           gi|700189476|gb|KGN44709.1| hypothetical protein
           Csa_7G373520 [Cucumis sativus]
          Length = 225

 Score = 82.4 bits (202), Expect = 1e-13
 Identities = 36/67 (53%), Positives = 50/67 (74%), Gaps = 1/67 (1%)
 Frame = -2

Query: 199 ILAVFIWSLFS-ESFVYAAQNDVDCLRVIRSSLKDPNGIFTTWDFNNKSNGFICTFIGID 23
           ++A F+  L S +SF +AA++D+ CLR I+ S +DPN   T+WDF+++S G IC FIGI 
Sbjct: 10  LVAGFVVMLLSCKSFTFAAESDLSCLRSIKKSFQDPNKYLTSWDFSSRSEGAICRFIGII 69

Query: 22  CWHPDEN 2
           CWHPDEN
Sbjct: 70  CWHPDEN 76


>ref|XP_009799665.1| PREDICTED: probably inactive leucine-rich repeat receptor-like
           protein kinase At5g48380 [Nicotiana sylvestris]
           gi|698508846|ref|XP_009799666.1| PREDICTED: probably
           inactive leucine-rich repeat receptor-like protein
           kinase At5g48380 [Nicotiana sylvestris]
          Length = 625

 Score = 82.4 bits (202), Expect = 1e-13
 Identities = 38/79 (48%), Positives = 54/79 (68%), Gaps = 1/79 (1%)
 Frame = -2

Query: 235 FMRIVLNYRALPI-LAVFIWSLFSESFVYAAQNDVDCLRVIRSSLKDPNGIFTTWDFNNK 59
           F ++V N  AL I  A  I+ L S +   A Q+D+DCL+ I+S+L+D +    +W+FNN+
Sbjct: 5   FKKMVSNCGALTIPAATLIYLLLSCTVCCALQSDIDCLKSIKSALEDTSNSLASWNFNNQ 64

Query: 58  SNGFICTFIGIDCWHPDEN 2
           + GFIC F GI+CWHPDEN
Sbjct: 65  TEGFICKFTGIECWHPDEN 83


>ref|XP_010101366.1| Probably inactive leucine-rich repeat receptor-like protein kinase
           [Morus notabilis] gi|587899942|gb|EXB88313.1| Probably
           inactive leucine-rich repeat receptor-like protein
           kinase [Morus notabilis]
          Length = 2189

 Score = 82.0 bits (201), Expect = 1e-13
 Identities = 36/70 (51%), Positives = 49/70 (70%), Gaps = 1/70 (1%)
 Frame = -2

Query: 208 ALPILAVFIW-SLFSESFVYAAQNDVDCLRVIRSSLKDPNGIFTTWDFNNKSNGFICTFI 32
           AL +L +F+  S+ S     A  +D+ CL+ I++SL+DP G   +WDFNN + GFIC FI
Sbjct: 8   ALALLRLFLLLSICSFGASRALVDDIHCLKAIKASLEDPYGYLASWDFNNNTEGFICKFI 67

Query: 31  GIDCWHPDEN 2
           G+DCWHPDEN
Sbjct: 68  GVDCWHPDEN 77


>emb|CDP03993.1| unnamed protein product [Coffea canephora]
          Length = 617

 Score = 81.6 bits (200), Expect = 2e-13
 Identities = 37/74 (50%), Positives = 48/74 (64%), Gaps = 1/74 (1%)
 Frame = -2

Query: 220 LNYRALPILA-VFIWSLFSESFVYAAQNDVDCLRVIRSSLKDPNGIFTTWDFNNKSNGFI 44
           L+ RAL  LA V  W   + SF      DVDCLR ++ +L+DP    +TW+F+N + GFI
Sbjct: 3   LHRRALTALAAVLAWFFANASFSNGNPRDVDCLRAVKQTLEDPLNSLSTWNFDNATEGFI 62

Query: 43  CTFIGIDCWHPDEN 2
           C F GI+CWHPDEN
Sbjct: 63  CKFTGIECWHPDEN 76


>ref|XP_007049531.1| Probably inactive leucine-rich repeat receptor-like protein kinase
           [Theobroma cacao] gi|508701792|gb|EOX93688.1| Probably
           inactive leucine-rich repeat receptor-like protein
           kinase [Theobroma cacao]
          Length = 619

 Score = 81.6 bits (200), Expect = 2e-13
 Identities = 33/67 (49%), Positives = 48/67 (71%), Gaps = 1/67 (1%)
 Frame = -2

Query: 199 ILAVFIWSLFSESFVYAAQNDVDCLRVIRSSLKDP-NGIFTTWDFNNKSNGFICTFIGID 23
           +++ F WSL   S  YA+  D++CL+ IR S +DP N + ++W+FNN + GFIC F G++
Sbjct: 13  LISAFAWSLLRCSLSYASVEDINCLKSIRDSFEDPFNYLNSSWNFNNDTEGFICRFTGVE 72

Query: 22  CWHPDEN 2
           CWHPDEN
Sbjct: 73  CWHPDEN 79


>ref|XP_007200186.1| hypothetical protein PRUPE_ppa016209mg [Prunus persica]
           gi|462395586|gb|EMJ01385.1| hypothetical protein
           PRUPE_ppa016209mg [Prunus persica]
          Length = 346

 Score = 81.6 bits (200), Expect = 2e-13
 Identities = 35/94 (37%), Positives = 61/94 (64%), Gaps = 2/94 (2%)
 Frame = -2

Query: 277 FVDILSFFGVMAAFFMRIVLNYRALPI-LAVFIWSLFSESFVYAAQNDVDCLRVIRSSLK 101
           F  +L+ + ++ AF M ++L  +   I +   +W L S  F +  ++D++CL+ I++SL+
Sbjct: 57  FYFLLAHYPILMAFLMHMLLRKQDAGIFIGSVLWLLLSCGFSFGVESDINCLKSIKASLE 116

Query: 100 DPNGIF-TTWDFNNKSNGFICTFIGIDCWHPDEN 2
           D  G   ++WDFNN + GFIC F+G++CWHP E+
Sbjct: 117 DTLGYLNSSWDFNNNTEGFICNFLGVECWHPHES 150


>ref|XP_010095584.1| Probably inactive leucine-rich repeat receptor-like protein kinase
           [Morus notabilis] gi|587871905|gb|EXB61157.1| Probably
           inactive leucine-rich repeat receptor-like protein
           kinase [Morus notabilis]
          Length = 602

 Score = 81.3 bits (199), Expect = 2e-13
 Identities = 36/84 (42%), Positives = 55/84 (65%), Gaps = 2/84 (2%)
 Frame = -2

Query: 247 MAAFFMRIVLNYRALPILA-VFIWSLFSESFVYAAQNDVDCLRVIRSSLKDPNGIF-TTW 74
           M  +   ++L+ R L I   + +W L   S   A+ ND++CL+ I+ SL+DP G   ++W
Sbjct: 1   MYIYAKNLLLSSRFLDISGGILLWLLIHSSLSLASVNDINCLKSIQESLEDPYGYLNSSW 60

Query: 73  DFNNKSNGFICTFIGIDCWHPDEN 2
           +FNN + G+IC FIG++CWHPDEN
Sbjct: 61  NFNNNTEGYICKFIGVECWHPDEN 84


>ref|XP_007200419.1| hypothetical protein PRUPE_ppa011049mg [Prunus persica]
           gi|462395819|gb|EMJ01618.1| hypothetical protein
           PRUPE_ppa011049mg [Prunus persica]
          Length = 225

 Score = 80.9 bits (198), Expect = 3e-13
 Identities = 33/81 (40%), Positives = 53/81 (65%), Gaps = 1/81 (1%)
 Frame = -2

Query: 241 AFFMRIVLNYRALPI-LAVFIWSLFSESFVYAAQNDVDCLRVIRSSLKDPNGIFTTWDFN 65
           AF M ++L  +   I +   +W L S  F +  ++D++CL+ I++SL+D  G   +WDFN
Sbjct: 2   AFLMHMLLRKQDAGIFIGSVLWLLLSCGFSFGVESDINCLKSIKASLEDTLGYLNSWDFN 61

Query: 64  NKSNGFICTFIGIDCWHPDEN 2
           N + GFIC F+G++CWHP E+
Sbjct: 62  NNTEGFICNFLGVECWHPHES 82