BLASTX nr result
ID: Forsythia23_contig00018479
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Forsythia23_contig00018479
(359 letters)
Database: ./nr
69,698,275 sequences; 24,982,196,650 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_011071764.1| PREDICTED: uncharacterized protein LOC105157... 135 1e-29
emb|CDP14924.1| unnamed protein product [Coffea canephora] 132 7e-29
ref|XP_012846826.1| PREDICTED: uncharacterized protein LOC105966... 130 3e-28
ref|XP_009777128.1| PREDICTED: uncharacterized protein LOC104226... 116 5e-24
ref|XP_009777127.1| PREDICTED: uncharacterized protein LOC104226... 116 5e-24
ref|XP_009630309.1| PREDICTED: uncharacterized protein LOC104120... 111 2e-22
ref|XP_009630308.1| PREDICTED: lisH domain-containing protein C1... 111 2e-22
ref|XP_009781378.1| PREDICTED: uncharacterized protein LOC104230... 108 1e-21
ref|XP_009781377.1| PREDICTED: uncharacterized protein LOC104230... 108 1e-21
ref|XP_006354813.1| PREDICTED: mediator of RNA polymerase II tra... 107 3e-21
ref|XP_004241517.1| PREDICTED: uncharacterized protein LOC101259... 106 5e-21
gb|ABI34275.1| hypothetical protein LES1_20t00015 [Solanum lycop... 106 5e-21
ref|XP_009776763.1| PREDICTED: cellulose synthase-like protein D... 106 7e-21
ref|XP_009776756.1| PREDICTED: uncharacterized protein LOC104226... 106 7e-21
ref|XP_009588335.1| PREDICTED: putative protein TPRXL isoform X2... 103 5e-20
ref|XP_009588334.1| PREDICTED: putative protein TPRXL isoform X1... 103 5e-20
ref|XP_002283833.1| PREDICTED: uncharacterized protein LOC100248... 101 2e-19
gb|ACJ06699.1| putative anti-virus transcriptional factor [Vitis... 101 2e-19
emb|CDP18150.1| unnamed protein product [Coffea canephora] 101 2e-19
gb|ACJ06697.1| putative anti-virus transcriptional factor [Vitis... 100 3e-19
>ref|XP_011071764.1| PREDICTED: uncharacterized protein LOC105157153 [Sesamum indicum]
Length = 367
Score = 135 bits (339), Expect = 1e-29
Identities = 69/122 (56%), Positives = 82/122 (67%), Gaps = 3/122 (2%)
Frame = -2
Query: 358 VADALAAGDDKQQQHNPDSGLDSSHLEK---DVXXXXXXXXXXXXNFGLDISKPKPENRG 188
+ADALAA +DKQQ+H P+SGLDS EK G DIS K E+ G
Sbjct: 173 MADALAATEDKQQEHIPNSGLDSPPSEKHGGSTKYGSQLSVKDRPFSGDDISNAKQESGG 232
Query: 187 MVAERVLVNCQAWRPDDACRPQSLPNLSKQYSFPMNSGRHFGCGGSVWACKNVGPVPTSC 8
++ L++ QAWRPDDA RPQSLPN+SKQ+SF ++S RHFG GGSVW CKNVGPVP SC
Sbjct: 233 TAIQKSLIHSQAWRPDDAFRPQSLPNMSKQHSFQLDSERHFGQGGSVWGCKNVGPVPKSC 292
Query: 7 PI 2
PI
Sbjct: 293 PI 294
>emb|CDP14924.1| unnamed protein product [Coffea canephora]
Length = 340
Score = 132 bits (333), Expect = 7e-29
Identities = 68/119 (57%), Positives = 81/119 (68%)
Frame = -2
Query: 358 VADALAAGDDKQQQHNPDSGLDSSHLEKDVXXXXXXXXXXXXNFGLDISKPKPENRGMVA 179
VADALAA D+KQQ H + S+ ++K++ +DI K KPEN G+
Sbjct: 162 VADALAATDEKQQLH-----IGSAPVDKNIAHFDSQSD-------VDILKEKPEN-GVSV 208
Query: 178 ERVLVNCQAWRPDDACRPQSLPNLSKQYSFPMNSGRHFGCGGSVWACKNVGPVPTSCPI 2
+R VNC+AWRPDDA RPQSLPNLSKQYSFPMNS RH+GCGGSVW CKN +P SCPI
Sbjct: 209 QRAPVNCRAWRPDDAFRPQSLPNLSKQYSFPMNSDRHYGCGGSVWGCKNAASIPKSCPI 267
>ref|XP_012846826.1| PREDICTED: uncharacterized protein LOC105966790 [Erythranthe
guttatus] gi|604317629|gb|EYU29508.1| hypothetical
protein MIMGU_mgv1a008470mg [Erythranthe guttata]
Length = 372
Score = 130 bits (327), Expect = 3e-28
Identities = 67/120 (55%), Positives = 78/120 (65%), Gaps = 1/120 (0%)
Frame = -2
Query: 358 VADALAAGDDKQQQ-HNPDSGLDSSHLEKDVXXXXXXXXXXXXNFGLDISKPKPENRGMV 182
VADALAA D KQQQ H P++G + E + G+ + E+ G
Sbjct: 186 VADALAASDKKQQQEHTPNTGSGLKNHENAIESGTQV--------GVGPTSANQESGGTA 237
Query: 181 AERVLVNCQAWRPDDACRPQSLPNLSKQYSFPMNSGRHFGCGGSVWACKNVGPVPTSCPI 2
ER LVNC AWRPDDACRPQSLPNL+KQYSFP+NS RH G G+VWACKN+GP PTSCPI
Sbjct: 238 KERSLVNCCAWRPDDACRPQSLPNLAKQYSFPLNSERHLGGRGAVWACKNLGPTPTSCPI 297
>ref|XP_009777128.1| PREDICTED: uncharacterized protein LOC104226766 isoform X2
[Nicotiana sylvestris]
Length = 315
Score = 116 bits (291), Expect = 5e-24
Identities = 66/119 (55%), Positives = 78/119 (65%)
Frame = -2
Query: 358 VADALAAGDDKQQQHNPDSGLDSSHLEKDVXXXXXXXXXXXXNFGLDISKPKPENRGMVA 179
VADALAA D K +QH+P GLDS + + G+D +K K E+RG+ A
Sbjct: 129 VADALAATDQKLEQHHP--GLDSPREKDENVHLSSRTNISNGVTGIDTTKQKSEHRGL-A 185
Query: 178 ERVLVNCQAWRPDDACRPQSLPNLSKQYSFPMNSGRHFGCGGSVWACKNVGPVPTSCPI 2
R V+C+AWRPDDA RPQ LPNLSKQYSFPMNS RH GG+VW CK + VPTSCPI
Sbjct: 186 SRPPVSCKAWRPDDAFRPQILPNLSKQYSFPMNSERHCR-GGAVWGCKRLS-VPTSCPI 242
>ref|XP_009777127.1| PREDICTED: uncharacterized protein LOC104226766 isoform X1
[Nicotiana sylvestris]
Length = 355
Score = 116 bits (291), Expect = 5e-24
Identities = 66/119 (55%), Positives = 78/119 (65%)
Frame = -2
Query: 358 VADALAAGDDKQQQHNPDSGLDSSHLEKDVXXXXXXXXXXXXNFGLDISKPKPENRGMVA 179
VADALAA D K +QH+P GLDS + + G+D +K K E+RG+ A
Sbjct: 169 VADALAATDQKLEQHHP--GLDSPREKDENVHLSSRTNISNGVTGIDTTKQKSEHRGL-A 225
Query: 178 ERVLVNCQAWRPDDACRPQSLPNLSKQYSFPMNSGRHFGCGGSVWACKNVGPVPTSCPI 2
R V+C+AWRPDDA RPQ LPNLSKQYSFPMNS RH GG+VW CK + VPTSCPI
Sbjct: 226 SRPPVSCKAWRPDDAFRPQILPNLSKQYSFPMNSERHCR-GGAVWGCKRLS-VPTSCPI 282
>ref|XP_009630309.1| PREDICTED: uncharacterized protein LOC104120269 isoform X2
[Nicotiana tomentosiformis]
Length = 315
Score = 111 bits (277), Expect = 2e-22
Identities = 65/119 (54%), Positives = 75/119 (63%)
Frame = -2
Query: 358 VADALAAGDDKQQQHNPDSGLDSSHLEKDVXXXXXXXXXXXXNFGLDISKPKPENRGMVA 179
VADALAA D K +Q +P GLDS + G+D +K K E+RG+ A
Sbjct: 129 VADALAATDQKLEQQHP--GLDSPRERDENVHLSSRTDVSNGVTGIDTTKQKSEHRGL-A 185
Query: 178 ERVLVNCQAWRPDDACRPQSLPNLSKQYSFPMNSGRHFGCGGSVWACKNVGPVPTSCPI 2
R V+C+AWR DDA RPQ LPNLSKQYSFPMNS RH GGSVW CK + VPTSCPI
Sbjct: 186 SRPPVSCKAWRSDDAFRPQILPNLSKQYSFPMNSERHCR-GGSVWGCKRLS-VPTSCPI 242
>ref|XP_009630308.1| PREDICTED: lisH domain-containing protein C1711.05-like isoform X1
[Nicotiana tomentosiformis]
Length = 355
Score = 111 bits (277), Expect = 2e-22
Identities = 65/119 (54%), Positives = 75/119 (63%)
Frame = -2
Query: 358 VADALAAGDDKQQQHNPDSGLDSSHLEKDVXXXXXXXXXXXXNFGLDISKPKPENRGMVA 179
VADALAA D K +Q +P GLDS + G+D +K K E+RG+ A
Sbjct: 169 VADALAATDQKLEQQHP--GLDSPRERDENVHLSSRTDVSNGVTGIDTTKQKSEHRGL-A 225
Query: 178 ERVLVNCQAWRPDDACRPQSLPNLSKQYSFPMNSGRHFGCGGSVWACKNVGPVPTSCPI 2
R V+C+AWR DDA RPQ LPNLSKQYSFPMNS RH GGSVW CK + VPTSCPI
Sbjct: 226 SRPPVSCKAWRSDDAFRPQILPNLSKQYSFPMNSERHCR-GGSVWGCKRLS-VPTSCPI 282
>ref|XP_009781378.1| PREDICTED: uncharacterized protein LOC104230311 isoform X2
[Nicotiana sylvestris]
Length = 298
Score = 108 bits (270), Expect = 1e-21
Identities = 65/119 (54%), Positives = 73/119 (61%)
Frame = -2
Query: 358 VADALAAGDDKQQQHNPDSGLDSSHLEKDVXXXXXXXXXXXXNFGLDISKPKPENRGMVA 179
VADALAA D KQ+QHNP LDS H EKD N + S + +A
Sbjct: 116 VADALAATDHKQEQHNPS--LDS-HCEKDENAAHMSSRQETSNRQVSESNDRE-----LA 167
Query: 178 ERVLVNCQAWRPDDACRPQSLPNLSKQYSFPMNSGRHFGCGGSVWACKNVGPVPTSCPI 2
R ++CQAWRPDD RP SLP LSKQYSFPMNS RH GGSVW CK++ VPTSCPI
Sbjct: 168 PRPPLSCQAWRPDDTFRPPSLPTLSKQYSFPMNSERHCR-GGSVWGCKSLSSVPTSCPI 225
>ref|XP_009781377.1| PREDICTED: uncharacterized protein LOC104230311 isoform X1
[Nicotiana sylvestris]
Length = 338
Score = 108 bits (270), Expect = 1e-21
Identities = 65/119 (54%), Positives = 73/119 (61%)
Frame = -2
Query: 358 VADALAAGDDKQQQHNPDSGLDSSHLEKDVXXXXXXXXXXXXNFGLDISKPKPENRGMVA 179
VADALAA D KQ+QHNP LDS H EKD N + S + +A
Sbjct: 156 VADALAATDHKQEQHNPS--LDS-HCEKDENAAHMSSRQETSNRQVSESNDRE-----LA 207
Query: 178 ERVLVNCQAWRPDDACRPQSLPNLSKQYSFPMNSGRHFGCGGSVWACKNVGPVPTSCPI 2
R ++CQAWRPDD RP SLP LSKQYSFPMNS RH GGSVW CK++ VPTSCPI
Sbjct: 208 PRPPLSCQAWRPDDTFRPPSLPTLSKQYSFPMNSERHCR-GGSVWGCKSLSSVPTSCPI 265
>ref|XP_006354813.1| PREDICTED: mediator of RNA polymerase II transcription subunit
1-like [Solanum tuberosum]
Length = 354
Score = 107 bits (267), Expect = 3e-21
Identities = 63/119 (52%), Positives = 76/119 (63%)
Frame = -2
Query: 358 VADALAAGDDKQQQHNPDSGLDSSHLEKDVXXXXXXXXXXXXNFGLDISKPKPENRGMVA 179
VADALAA D +Q+QHN S LDS+ +V +D S+ P+ RG +
Sbjct: 171 VADALAATDKEQEQHN--SSLDSA---PEVHMSFQQEVSDGKVPVMDTSQQNPKGRGTIP 225
Query: 178 ERVLVNCQAWRPDDACRPQSLPNLSKQYSFPMNSGRHFGCGGSVWACKNVGPVPTSCPI 2
V+ QAWRPDD RPQSLPNLSKQY+FPMNSGRH+ GGSVW CK+ +PTSCPI
Sbjct: 226 G-FPVSFQAWRPDDEFRPQSLPNLSKQYTFPMNSGRHYR-GGSVWGCKSAA-IPTSCPI 281
>ref|XP_004241517.1| PREDICTED: uncharacterized protein LOC101259850 [Solanum
lycopersicum]
Length = 354
Score = 106 bits (265), Expect = 5e-21
Identities = 64/119 (53%), Positives = 74/119 (62%)
Frame = -2
Query: 358 VADALAAGDDKQQQHNPDSGLDSSHLEKDVXXXXXXXXXXXXNFGLDISKPKPENRGMVA 179
VADALAA D +Q+QHN S LDS+ V LD S+ P+ RG +
Sbjct: 171 VADALAATDKEQEQHN--SSLDSA---PQVHMSFQQEVSDGKVPALDTSQQNPKGRGTIP 225
Query: 178 ERVLVNCQAWRPDDACRPQSLPNLSKQYSFPMNSGRHFGCGGSVWACKNVGPVPTSCPI 2
V+ QAWRPDD RPQSLPNLSKQY+FPMNSGRH GGSVW CK+ +PTSCPI
Sbjct: 226 V-FPVSFQAWRPDDEFRPQSLPNLSKQYTFPMNSGRHCR-GGSVWGCKSAA-IPTSCPI 281
>gb|ABI34275.1| hypothetical protein LES1_20t00015 [Solanum lycopersicum]
Length = 309
Score = 106 bits (265), Expect = 5e-21
Identities = 64/119 (53%), Positives = 74/119 (62%)
Frame = -2
Query: 358 VADALAAGDDKQQQHNPDSGLDSSHLEKDVXXXXXXXXXXXXNFGLDISKPKPENRGMVA 179
VADALAA D +Q+QHN S LDS+ V LD S+ P+ RG +
Sbjct: 126 VADALAATDKEQEQHN--SSLDSA---PQVHMSFQQEVSDGKVPALDTSQQNPKGRGTIP 180
Query: 178 ERVLVNCQAWRPDDACRPQSLPNLSKQYSFPMNSGRHFGCGGSVWACKNVGPVPTSCPI 2
V+ QAWRPDD RPQSLPNLSKQY+FPMNSGRH GGSVW CK+ +PTSCPI
Sbjct: 181 V-FPVSFQAWRPDDEFRPQSLPNLSKQYTFPMNSGRHCR-GGSVWGCKSAA-IPTSCPI 236
>ref|XP_009776763.1| PREDICTED: cellulose synthase-like protein D2 isoform X2 [Nicotiana
sylvestris]
Length = 313
Score = 106 bits (264), Expect = 7e-21
Identities = 64/120 (53%), Positives = 78/120 (65%), Gaps = 1/120 (0%)
Frame = -2
Query: 358 VADALAAGDDKQQQHNPDSGLDSS-HLEKDVXXXXXXXXXXXXNFGLDISKPKPENRGMV 182
VADALAA D+KQ+Q + S LDS+ +++V GLDISK K
Sbjct: 130 VADALAATDEKQEQLS--SSLDSAPERDENVVHVSSPEDPDQPESGLDISKQKSRE---- 183
Query: 181 AERVLVNCQAWRPDDACRPQSLPNLSKQYSFPMNSGRHFGCGGSVWACKNVGPVPTSCPI 2
R V+ +AWRPDDA RPQSLPNLSKQY+FPMN GRH+ GGSVW CK++ +PTSCPI
Sbjct: 184 -PRSPVSFRAWRPDDAFRPQSLPNLSKQYTFPMNLGRHYR-GGSVWGCKSLS-IPTSCPI 240
>ref|XP_009776756.1| PREDICTED: uncharacterized protein LOC104226440 isoform X1
[Nicotiana sylvestris]
Length = 353
Score = 106 bits (264), Expect = 7e-21
Identities = 64/120 (53%), Positives = 78/120 (65%), Gaps = 1/120 (0%)
Frame = -2
Query: 358 VADALAAGDDKQQQHNPDSGLDSS-HLEKDVXXXXXXXXXXXXNFGLDISKPKPENRGMV 182
VADALAA D+KQ+Q + S LDS+ +++V GLDISK K
Sbjct: 170 VADALAATDEKQEQLS--SSLDSAPERDENVVHVSSPEDPDQPESGLDISKQKSRE---- 223
Query: 181 AERVLVNCQAWRPDDACRPQSLPNLSKQYSFPMNSGRHFGCGGSVWACKNVGPVPTSCPI 2
R V+ +AWRPDDA RPQSLPNLSKQY+FPMN GRH+ GGSVW CK++ +PTSCPI
Sbjct: 224 -PRSPVSFRAWRPDDAFRPQSLPNLSKQYTFPMNLGRHYR-GGSVWGCKSLS-IPTSCPI 280
>ref|XP_009588335.1| PREDICTED: putative protein TPRXL isoform X2 [Nicotiana
tomentosiformis]
Length = 300
Score = 103 bits (257), Expect = 5e-20
Identities = 64/119 (53%), Positives = 74/119 (62%)
Frame = -2
Query: 358 VADALAAGDDKQQQHNPDSGLDSSHLEKDVXXXXXXXXXXXXNFGLDISKPKPENRGMVA 179
VADALAA D KQ+QHNP LDS H E+D N + S +RG+ A
Sbjct: 118 VADALAATDHKQEQHNPS--LDS-HCERDDNVAHMSSRQVTSNRQVSESN----DRGL-A 169
Query: 178 ERVLVNCQAWRPDDACRPQSLPNLSKQYSFPMNSGRHFGCGGSVWACKNVGPVPTSCPI 2
R ++ QAWRPDD RP SLP LSKQYSFPMNS RH GGSVW CK++ +PTSCPI
Sbjct: 170 PRPPLSSQAWRPDDTFRPPSLPTLSKQYSFPMNSERHCR-GGSVWGCKSLSSIPTSCPI 227
>ref|XP_009588334.1| PREDICTED: putative protein TPRXL isoform X1 [Nicotiana
tomentosiformis]
Length = 340
Score = 103 bits (257), Expect = 5e-20
Identities = 64/119 (53%), Positives = 74/119 (62%)
Frame = -2
Query: 358 VADALAAGDDKQQQHNPDSGLDSSHLEKDVXXXXXXXXXXXXNFGLDISKPKPENRGMVA 179
VADALAA D KQ+QHNP LDS H E+D N + S +RG+ A
Sbjct: 158 VADALAATDHKQEQHNPS--LDS-HCERDDNVAHMSSRQVTSNRQVSESN----DRGL-A 209
Query: 178 ERVLVNCQAWRPDDACRPQSLPNLSKQYSFPMNSGRHFGCGGSVWACKNVGPVPTSCPI 2
R ++ QAWRPDD RP SLP LSKQYSFPMNS RH GGSVW CK++ +PTSCPI
Sbjct: 210 PRPPLSSQAWRPDDTFRPPSLPTLSKQYSFPMNSERHCR-GGSVWGCKSLSSIPTSCPI 267
>ref|XP_002283833.1| PREDICTED: uncharacterized protein LOC100248510 [Vitis vinifera]
Length = 348
Score = 101 bits (252), Expect = 2e-19
Identities = 59/119 (49%), Positives = 69/119 (57%)
Frame = -2
Query: 358 VADALAAGDDKQQQHNPDSGLDSSHLEKDVXXXXXXXXXXXXNFGLDISKPKPENRGMVA 179
VADALAA D ++ SH+E+ + GL I KPE+ GMV
Sbjct: 158 VADALAAVDKQKIPVQEGHVEQESHVEQKPVVRSGSPGGISKDTGLGIEIHKPEHVGMVP 217
Query: 178 ERVLVNCQAWRPDDACRPQSLPNLSKQYSFPMNSGRHFGCGGSVWACKNVGPVPTSCPI 2
R N QAWR DDA RPQSLPNLSKQ+SFPMNS RH+G GG WA +V +P SCPI
Sbjct: 218 -RAPANGQAWRADDAFRPQSLPNLSKQHSFPMNSDRHYGHGGVPWARSSVAAIPISCPI 275
>gb|ACJ06699.1| putative anti-virus transcriptional factor [Vitis pseudoreticulata]
Length = 348
Score = 101 bits (252), Expect = 2e-19
Identities = 59/119 (49%), Positives = 69/119 (57%)
Frame = -2
Query: 358 VADALAAGDDKQQQHNPDSGLDSSHLEKDVXXXXXXXXXXXXNFGLDISKPKPENRGMVA 179
VADALAA D ++ SH+E+ + GL I KPE+ GMV
Sbjct: 158 VADALAAVDKQKIPVQEGHVEQESHVEQKPVVRSGSPGGISKDTGLGIEIHKPEHVGMVP 217
Query: 178 ERVLVNCQAWRPDDACRPQSLPNLSKQYSFPMNSGRHFGCGGSVWACKNVGPVPTSCPI 2
R N QAWR DDA RPQSLPNLSKQ+SFPMNS RH+G GG WA +V +P SCPI
Sbjct: 218 -RAPANGQAWRADDAFRPQSLPNLSKQHSFPMNSDRHYGHGGVPWARSSVAAIPISCPI 275
>emb|CDP18150.1| unnamed protein product [Coffea canephora]
Length = 219
Score = 101 bits (251), Expect = 2e-19
Identities = 57/119 (47%), Positives = 67/119 (56%)
Frame = -2
Query: 358 VADALAAGDDKQQQHNPDSGLDSSHLEKDVXXXXXXXXXXXXNFGLDISKPKPENRGMVA 179
VADALAA D+KQQ H + +D + D +DISK KPEN G+
Sbjct: 94 VADALAATDEKQQLHIGTAPVDKNIAHFDSQSD------------VDISKEKPEN-GVSV 140
Query: 178 ERVLVNCQAWRPDDACRPQSLPNLSKQYSFPMNSGRHFGCGGSVWACKNVGPVPTSCPI 2
+R VNC+AWRPDDA RPQS PNL H+GCGGSVW CKN +P SCPI
Sbjct: 141 QRAPVNCRAWRPDDAFRPQSWPNL------------HYGCGGSVWGCKNAASIPKSCPI 187
>gb|ACJ06697.1| putative anti-virus transcriptional factor [Vitis pseudoreticulata]
Length = 350
Score = 100 bits (250), Expect = 3e-19
Identities = 59/119 (49%), Positives = 69/119 (57%)
Frame = -2
Query: 358 VADALAAGDDKQQQHNPDSGLDSSHLEKDVXXXXXXXXXXXXNFGLDISKPKPENRGMVA 179
VADALAA D ++ SH+E+ + GL I KPE+ GMV
Sbjct: 160 VADALAAVDKQKIPVQEGHVERESHVEQKPVVRSGSPGGISKDTGLGIEIHKPEHVGMVP 219
Query: 178 ERVLVNCQAWRPDDACRPQSLPNLSKQYSFPMNSGRHFGCGGSVWACKNVGPVPTSCPI 2
R N QAWR DDA RPQSLPNLSKQ+SFPMNS RH+G GG WA +V +P SCPI
Sbjct: 220 -RAPANGQAWRADDAFRPQSLPNLSKQHSFPMNSDRHYGHGGVPWARSSVAAIPISCPI 277