BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Forsythia23_contig00004520
         (330 letters)

Database: ./nr 
           69,698,275 sequences; 24,982,196,650 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CDP18288.1| unnamed protein product [Coffea canephora]            174   2e-41
ref|XP_011069614.1| PREDICTED: pathogen-related protein-like [Se...   172   6e-41
ref|XP_009626199.1| PREDICTED: pathogen-related protein-like [Ni...   167   3e-39
gb|AGW81767.1| EF1 protein [Ipomoea batatas]                          167   3e-39
gb|AGW81769.1| EF2 protein [Nicotiana tabacum]                        165   1e-38
ref|XP_009795903.1| PREDICTED: pathogen-related protein-like iso...   160   3e-37
ref|XP_009795902.1| PREDICTED: pathogen-related protein-like iso...   159   7e-37
ref|XP_004237745.1| PREDICTED: pathogen-related protein [Solanum...   158   1e-36
ref|XP_011084185.1| PREDICTED: pathogen-related protein-like [Se...   157   3e-36
ref|XP_012829495.1| PREDICTED: pathogen-related protein-like [Er...   157   3e-36
ref|XP_008373181.1| PREDICTED: pathogen-related protein-like [Ma...   157   3e-36
ref|XP_009626198.1| PREDICTED: pathogen-related protein-like [Ni...   156   5e-36
ref|XP_009360748.1| PREDICTED: pathogen-related protein-like iso...   156   6e-36
ref|XP_009360747.1| PREDICTED: pathogen-related protein-like iso...   156   6e-36
ref|XP_006356272.1| PREDICTED: pathogen-related protein-like [So...   156   6e-36
ref|XP_004237744.1| PREDICTED: pathogen-related protein [Solanum...   155   1e-35
ref|XP_009795904.1| PREDICTED: pathogen-related protein-like iso...   154   3e-35
ref|XP_006356275.1| PREDICTED: pathogen-related protein-like [So...   153   5e-35
ref|XP_008237647.1| PREDICTED: pathogen-related protein-like [Pr...   152   7e-35
ref|XP_007200367.1| hypothetical protein PRUPE_ppa010660mg [Prun...   152   1e-34

>emb|CDP18288.1| unnamed protein product [Coffea canephora]
          Length = 234

 Score =  174 bits (442), Expect = 2e-41
 Identities = 84/109 (77%), Positives = 92/109 (84%)
 Frame = -3

Query: 328 LFEEGRTNIWPEGSLEEKVQRLVKTWEMELVHKADPDQYKTLDAKKFKLGVNGRKFLTLE 149
           LFEEGRT IWP GSLEEKVQRLVKTWEME+VHK +PD+YKTLDAKKF LG+NGRK LTLE
Sbjct: 36  LFEEGRTKIWPVGSLEEKVQRLVKTWEMEIVHKVNPDEYKTLDAKKFTLGINGRKALTLE 95

Query: 148 EIIKVGGSYNVFLQTSLPANLRFYNPEEETADSSQTLFKSTLPRGFAVE 2
           E  K+GGSYNVFLQT LP N R YNP  ETA+SSQ +F +T  RGFAVE
Sbjct: 96  EAGKLGGSYNVFLQTGLPENFRVYNPANETAESSQKVFTTTFARGFAVE 144


>ref|XP_011069614.1| PREDICTED: pathogen-related protein-like [Sesamum indicum]
          Length = 238

 Score =  172 bits (437), Expect = 6e-41
 Identities = 81/109 (74%), Positives = 94/109 (86%)
 Frame = -3

Query: 328 LFEEGRTNIWPEGSLEEKVQRLVKTWEMELVHKADPDQYKTLDAKKFKLGVNGRKFLTLE 149
           LFEEGRT +WP GSLEEKVQ LVKTWEMELVHKA+PD YKTLDA KF LG+NG+K+ TLE
Sbjct: 38  LFEEGRTKVWPVGSLEEKVQNLVKTWEMELVHKANPDDYKTLDANKFVLGINGKKYFTLE 97

Query: 148 EIIKVGGSYNVFLQTSLPANLRFYNPEEETADSSQTLFKSTLPRGFAVE 2
           E+ KVGGSYNVFLQTSLP  LR Y+P++E+ +SSQ+ FK+  PRGFAVE
Sbjct: 98  EVGKVGGSYNVFLQTSLPPALRSYDPDQESYESSQSAFKTVFPRGFAVE 146


>ref|XP_009626199.1| PREDICTED: pathogen-related protein-like [Nicotiana
           tomentosiformis]
          Length = 234

 Score =  167 bits (422), Expect = 3e-39
 Identities = 78/109 (71%), Positives = 94/109 (86%)
 Frame = -3

Query: 328 LFEEGRTNIWPEGSLEEKVQRLVKTWEMELVHKADPDQYKTLDAKKFKLGVNGRKFLTLE 149
           LFEE RT++WPEGSLEEKVQRL+KTWEME+VHKADP+++KT+D KKFK+ VNGRK LT  
Sbjct: 38  LFEEERTHVWPEGSLEEKVQRLLKTWEMEIVHKADPNEFKTVDPKKFKISVNGRKGLTPV 97

Query: 148 EIIKVGGSYNVFLQTSLPANLRFYNPEEETADSSQTLFKSTLPRGFAVE 2
           E  K+GGSYN+FLQTSLP NLR YNP+EET +SSQ +F+S   RGFA+E
Sbjct: 98  ESAKLGGSYNIFLQTSLPENLRVYNPDEETFESSQKVFRSIFLRGFAIE 146


>gb|AGW81767.1| EF1 protein [Ipomoea batatas]
          Length = 221

 Score =  167 bits (422), Expect = 3e-39
 Identities = 79/109 (72%), Positives = 91/109 (83%)
 Frame = -3

Query: 328 LFEEGRTNIWPEGSLEEKVQRLVKTWEMELVHKADPDQYKTLDAKKFKLGVNGRKFLTLE 149
           LFEEGRT++WPEGSLEEKVQRL+KTWEMELVHKADP+ YKTLD  KF+L VNGRK L+LE
Sbjct: 38  LFEEGRTHVWPEGSLEEKVQRLMKTWEMELVHKADPNDYKTLDPTKFRLFVNGRKGLSLE 97

Query: 148 EIIKVGGSYNVFLQTSLPANLRFYNPEEETADSSQTLFKSTLPRGFAVE 2
           E  K+GGSYNVFLQTSLP   R +NP +ET  SSQ  F++  PRGFA+E
Sbjct: 98  ETAKIGGSYNVFLQTSLPEKYRVFNPADETFVSSQAAFRNAFPRGFAIE 146


>gb|AGW81769.1| EF2 protein [Nicotiana tabacum]
          Length = 242

 Score =  165 bits (418), Expect = 1e-38
 Identities = 77/109 (70%), Positives = 94/109 (86%)
 Frame = -3

Query: 328 LFEEGRTNIWPEGSLEEKVQRLVKTWEMELVHKADPDQYKTLDAKKFKLGVNGRKFLTLE 149
           LFEE RT++WPEGSLEEKVQRL+KTWEME+VHKADP+++KT+D KKFK+ VNGRK LT  
Sbjct: 46  LFEEERTHVWPEGSLEEKVQRLLKTWEMEIVHKADPNEFKTVDPKKFKISVNGRKGLTPV 105

Query: 148 EIIKVGGSYNVFLQTSLPANLRFYNPEEETADSSQTLFKSTLPRGFAVE 2
           E  K+GGSYN+FLQTSLP +LR YNP+EET +SSQ +F+S   RGFA+E
Sbjct: 106 ESAKLGGSYNIFLQTSLPESLRIYNPDEETFESSQKVFRSIFLRGFAIE 154


>ref|XP_009795903.1| PREDICTED: pathogen-related protein-like isoform X2 [Nicotiana
           sylvestris]
          Length = 234

 Score =  160 bits (405), Expect = 3e-37
 Identities = 74/109 (67%), Positives = 92/109 (84%)
 Frame = -3

Query: 328 LFEEGRTNIWPEGSLEEKVQRLVKTWEMELVHKADPDQYKTLDAKKFKLGVNGRKFLTLE 149
           LFEE RT++WPEGSLEEKVQRL+KTWEME+VHKADP+ +KT+D  KFK+ +NGRK LT  
Sbjct: 38  LFEEERTHVWPEGSLEEKVQRLLKTWEMEIVHKADPNDFKTVDPTKFKISINGRKGLTPV 97

Query: 148 EIIKVGGSYNVFLQTSLPANLRFYNPEEETADSSQTLFKSTLPRGFAVE 2
           E  K+GGSYN+FLQTSLP ++R YNP+EET +SSQ +F+S   RGFA+E
Sbjct: 98  ESAKLGGSYNIFLQTSLPESVRVYNPDEETFESSQKVFRSIFLRGFAIE 146


>ref|XP_009795902.1| PREDICTED: pathogen-related protein-like isoform X1 [Nicotiana
           sylvestris]
          Length = 234

 Score =  159 bits (402), Expect = 7e-37
 Identities = 73/109 (66%), Positives = 92/109 (84%)
 Frame = -3

Query: 328 LFEEGRTNIWPEGSLEEKVQRLVKTWEMELVHKADPDQYKTLDAKKFKLGVNGRKFLTLE 149
           LFE+ RT++WPEGSLEEKVQRL+KTWEME+VHKADP+ +KT+D  KFK+ +NGRK LT  
Sbjct: 38  LFEQERTHVWPEGSLEEKVQRLLKTWEMEIVHKADPNDFKTVDPTKFKISINGRKGLTPV 97

Query: 148 EIIKVGGSYNVFLQTSLPANLRFYNPEEETADSSQTLFKSTLPRGFAVE 2
           E  K+GGSYN+FLQTSLP ++R YNP+EET +SSQ +F+S   RGFA+E
Sbjct: 98  ESAKLGGSYNIFLQTSLPESVRVYNPDEETFESSQKVFRSIFLRGFAIE 146


>ref|XP_004237745.1| PREDICTED: pathogen-related protein [Solanum lycopersicum]
          Length = 257

 Score =  158 bits (400), Expect = 1e-36
 Identities = 73/109 (66%), Positives = 90/109 (82%)
 Frame = -3

Query: 328 LFEEGRTNIWPEGSLEEKVQRLVKTWEMELVHKADPDQYKTLDAKKFKLGVNGRKFLTLE 149
           LFE+ RTN+W EGS+EEKVQRL+KTWEME+VHKADP++ KT+D  KF + VNGRK LT  
Sbjct: 66  LFEQERTNVWAEGSVEEKVQRLLKTWEMEIVHKADPNELKTMDPNKFTISVNGRKRLTSA 125

Query: 148 EIIKVGGSYNVFLQTSLPANLRFYNPEEETADSSQTLFKSTLPRGFAVE 2
           E  K+GGSYN+FLQTSLP N+R YNP++ET +SSQ LF+S   RGFA+E
Sbjct: 126 ESAKLGGSYNIFLQTSLPENVRIYNPDDETFESSQNLFRSIFLRGFAIE 174


>ref|XP_011084185.1| PREDICTED: pathogen-related protein-like [Sesamum indicum]
          Length = 234

 Score =  157 bits (397), Expect = 3e-36
 Identities = 71/109 (65%), Positives = 89/109 (81%)
 Frame = -3

Query: 328 LFEEGRTNIWPEGSLEEKVQRLVKTWEMELVHKADPDQYKTLDAKKFKLGVNGRKFLTLE 149
           LFEEGRT IW  GSLE++VQ LVKTWEME +HKA+PD YKT+DAKK+ +G+NG+++ TLE
Sbjct: 39  LFEEGRTKIWAAGSLEDQVQNLVKTWEMESIHKANPDDYKTVDAKKYVVGINGKRYFTLE 98

Query: 148 EIIKVGGSYNVFLQTSLPANLRFYNPEEETADSSQTLFKSTLPRGFAVE 2
           E  ++GG YNVFLQTSLP  LR Y+P+ ET +SSQ  FK+  PRGFA+E
Sbjct: 99  EARRIGGGYNVFLQTSLPPALRSYDPDRETYESSQAAFKTVFPRGFALE 147


>ref|XP_012829495.1| PREDICTED: pathogen-related protein-like [Erythranthe guttatus]
           gi|604297156|gb|EYU17420.1| hypothetical protein
           MIMGU_mgv1a012039mg [Erythranthe guttata]
          Length = 263

 Score =  157 bits (397), Expect = 3e-36
 Identities = 76/109 (69%), Positives = 89/109 (81%)
 Frame = -3

Query: 328 LFEEGRTNIWPEGSLEEKVQRLVKTWEMELVHKADPDQYKTLDAKKFKLGVNGRKFLTLE 149
           LFEEGRT++W  GSLEE VQ LVKTWE ELV KADP+ YKT+    F LG+NG+K+ TLE
Sbjct: 69  LFEEGRTHVWEAGSLEEYVQSLVKTWEFELVQKADPNDYKTV-VPNFILGINGKKYFTLE 127

Query: 148 EIIKVGGSYNVFLQTSLPANLRFYNPEEETADSSQTLFKSTLPRGFAVE 2
           +I K+GGSYNVFLQTSLP +LRFYNP+EET +SSQT+FK   PRGFA E
Sbjct: 128 QIGKIGGSYNVFLQTSLPPSLRFYNPDEETYNSSQTVFKQVFPRGFAFE 176


>ref|XP_008373181.1| PREDICTED: pathogen-related protein-like [Malus domestica]
          Length = 238

 Score =  157 bits (396), Expect = 3e-36
 Identities = 74/109 (67%), Positives = 87/109 (79%)
 Frame = -3

Query: 328 LFEEGRTNIWPEGSLEEKVQRLVKTWEMELVHKADPDQYKTLDAKKFKLGVNGRKFLTLE 149
           LFEEGRT IWP GSLEE+VQ LVKTWEMEL HK++PD +KTLD  K+ + VNGRK + +E
Sbjct: 39  LFEEGRTKIWPPGSLEEEVQNLVKTWEMELFHKSNPDDFKTLDPNKYTVSVNGRKGINIE 98

Query: 148 EIIKVGGSYNVFLQTSLPANLRFYNPEEETADSSQTLFKSTLPRGFAVE 2
           EI K+GG YN FLQTSLP  LR YNP+EETA+SS   F +T PRGFA+E
Sbjct: 99  EIGKIGGGYNSFLQTSLPEKLRGYNPDEETAESSLKAFTTTFPRGFALE 147


>ref|XP_009626198.1| PREDICTED: pathogen-related protein-like [Nicotiana
           tomentosiformis]
          Length = 238

 Score =  156 bits (395), Expect = 5e-36
 Identities = 74/109 (67%), Positives = 88/109 (80%)
 Frame = -3

Query: 328 LFEEGRTNIWPEGSLEEKVQRLVKTWEMELVHKADPDQYKTLDAKKFKLGVNGRKFLTLE 149
           LFEE RT++W EGSLEEKVQRL+KTWEME+VHK +P+ +KT+D KKF   VNGRK LT E
Sbjct: 38  LFEEERTHVWSEGSLEEKVQRLLKTWEMEMVHKENPNDFKTVDPKKFTKSVNGRKGLTAE 97

Query: 148 EIIKVGGSYNVFLQTSLPANLRFYNPEEETADSSQTLFKSTLPRGFAVE 2
           E  K+GG YN FLQTSLP NLR YNP+EET +SSQ +F+S   RGFA+E
Sbjct: 98  ENAKLGGGYNTFLQTSLPENLRIYNPDEETFESSQKVFRSIFVRGFAIE 146


>ref|XP_009360748.1| PREDICTED: pathogen-related protein-like isoform X2 [Pyrus x
           bretschneideri]
          Length = 204

 Score =  156 bits (394), Expect = 6e-36
 Identities = 73/109 (66%), Positives = 87/109 (79%)
 Frame = -3

Query: 328 LFEEGRTNIWPEGSLEEKVQRLVKTWEMELVHKADPDQYKTLDAKKFKLGVNGRKFLTLE 149
           LFEEGRT IWP GSLEE+VQ LVKTWEMEL HK++PD +KTLD  K+ + +NGRK + +E
Sbjct: 39  LFEEGRTKIWPPGSLEEEVQNLVKTWEMELFHKSNPDDFKTLDPNKYTVSLNGRKGINIE 98

Query: 148 EIIKVGGSYNVFLQTSLPANLRFYNPEEETADSSQTLFKSTLPRGFAVE 2
           EI K+GG YN FLQTSLP  LR YNP+EETA+SS   F +T PRGFA+E
Sbjct: 99  EIGKIGGGYNAFLQTSLPEKLRGYNPDEETAESSLKSFTTTFPRGFALE 147


>ref|XP_009360747.1| PREDICTED: pathogen-related protein-like isoform X1 [Pyrus x
           bretschneideri]
          Length = 238

 Score =  156 bits (394), Expect = 6e-36
 Identities = 73/109 (66%), Positives = 87/109 (79%)
 Frame = -3

Query: 328 LFEEGRTNIWPEGSLEEKVQRLVKTWEMELVHKADPDQYKTLDAKKFKLGVNGRKFLTLE 149
           LFEEGRT IWP GSLEE+VQ LVKTWEMEL HK++PD +KTLD  K+ + +NGRK + +E
Sbjct: 39  LFEEGRTKIWPPGSLEEEVQNLVKTWEMELFHKSNPDDFKTLDPNKYTVSLNGRKGINIE 98

Query: 148 EIIKVGGSYNVFLQTSLPANLRFYNPEEETADSSQTLFKSTLPRGFAVE 2
           EI K+GG YN FLQTSLP  LR YNP+EETA+SS   F +T PRGFA+E
Sbjct: 99  EIGKIGGGYNAFLQTSLPEKLRGYNPDEETAESSLKSFTTTFPRGFALE 147


>ref|XP_006356272.1| PREDICTED: pathogen-related protein-like [Solanum tuberosum]
          Length = 230

 Score =  156 bits (394), Expect = 6e-36
 Identities = 71/109 (65%), Positives = 90/109 (82%)
 Frame = -3

Query: 328 LFEEGRTNIWPEGSLEEKVQRLVKTWEMELVHKADPDQYKTLDAKKFKLGVNGRKFLTLE 149
           LFE+ RTN+W EGS+EE+VQRL+KTWEME+VHKADP++ KT+D  KF + +NGRK LT  
Sbjct: 39  LFEQERTNVWAEGSVEEEVQRLLKTWEMEIVHKADPNELKTMDPNKFTISINGRKGLTPA 98

Query: 148 EIIKVGGSYNVFLQTSLPANLRFYNPEEETADSSQTLFKSTLPRGFAVE 2
           E  K+GGSYN+FLQTSLP N+R YNP++ET +SSQ LF+S   RGFA+E
Sbjct: 99  ESAKLGGSYNIFLQTSLPENVRLYNPDDETFESSQNLFRSIFLRGFAIE 147


>ref|XP_004237744.1| PREDICTED: pathogen-related protein [Solanum lycopersicum]
          Length = 240

 Score =  155 bits (391), Expect = 1e-35
 Identities = 72/109 (66%), Positives = 89/109 (81%)
 Frame = -3

Query: 328 LFEEGRTNIWPEGSLEEKVQRLVKTWEMELVHKADPDQYKTLDAKKFKLGVNGRKFLTLE 149
           LFE+ RT++W EGS+E+KVQRL+KTWEMELVHK DP++ K++D  KFK  VNGRK LT E
Sbjct: 39  LFEQERTHVWAEGSVEDKVQRLLKTWEMELVHKIDPNEMKSVDPTKFKKSVNGRKGLTPE 98

Query: 148 EIIKVGGSYNVFLQTSLPANLRFYNPEEETADSSQTLFKSTLPRGFAVE 2
           E +K+GG YN FLQTSLP NLR YNPE+ET +SSQ +F+S   RGFA+E
Sbjct: 99  EAVKLGGGYNTFLQTSLPENLRVYNPEDETFESSQNVFRSIFVRGFAIE 147


>ref|XP_009795904.1| PREDICTED: pathogen-related protein-like isoform X3 [Nicotiana
           sylvestris]
          Length = 234

 Score =  154 bits (388), Expect = 3e-35
 Identities = 72/109 (66%), Positives = 88/109 (80%)
 Frame = -3

Query: 328 LFEEGRTNIWPEGSLEEKVQRLVKTWEMELVHKADPDQYKTLDAKKFKLGVNGRKFLTLE 149
           LFEE RT++WPEGSLEEKVQRL+KTWEME+VHK DP+ +KT+D K +   VNGRK LT E
Sbjct: 38  LFEEERTHVWPEGSLEEKVQRLLKTWEMEMVHKTDPNDFKTVDPKVYTKSVNGRKGLTPE 97

Query: 148 EIIKVGGSYNVFLQTSLPANLRFYNPEEETADSSQTLFKSTLPRGFAVE 2
           E  K+GG YN FLQTSLP ++R YNP+EET +SSQ +F+S   RGFA+E
Sbjct: 98  ENAKLGGGYNTFLQTSLPESVRVYNPDEETFESSQKIFRSIFLRGFAIE 146


>ref|XP_006356275.1| PREDICTED: pathogen-related protein-like [Solanum tuberosum]
          Length = 239

 Score =  153 bits (386), Expect = 5e-35
 Identities = 72/109 (66%), Positives = 88/109 (80%)
 Frame = -3

Query: 328 LFEEGRTNIWPEGSLEEKVQRLVKTWEMELVHKADPDQYKTLDAKKFKLGVNGRKFLTLE 149
           LFE+ RT++W EGS+E+KVQRL+KTWEMELVHK DP+  K++D  KFK  VNGRK LT E
Sbjct: 38  LFEQERTHVWAEGSVEDKVQRLLKTWEMELVHKIDPNDMKSVDPTKFKKSVNGRKGLTPE 97

Query: 148 EIIKVGGSYNVFLQTSLPANLRFYNPEEETADSSQTLFKSTLPRGFAVE 2
           E IK+GG YN FLQTSLP NLR YNP++ET +SSQ +F+S   RGFA+E
Sbjct: 98  EAIKLGGGYNTFLQTSLPENLRVYNPDDETFESSQNVFRSIFIRGFAIE 146


>ref|XP_008237647.1| PREDICTED: pathogen-related protein-like [Prunus mume]
          Length = 241

 Score =  152 bits (385), Expect = 7e-35
 Identities = 73/109 (66%), Positives = 86/109 (78%)
 Frame = -3

Query: 328 LFEEGRTNIWPEGSLEEKVQRLVKTWEMELVHKADPDQYKTLDAKKFKLGVNGRKFLTLE 149
           LFEEGRT IWP GSLE++VQ LVKTWEMEL HKA+ D +KTLD  K+   +NGRK +TLE
Sbjct: 42  LFEEGRTKIWPPGSLEDEVQNLVKTWEMELFHKANLDDFKTLDPNKYTFSLNGRKGITLE 101

Query: 148 EIIKVGGSYNVFLQTSLPANLRFYNPEEETADSSQTLFKSTLPRGFAVE 2
           EI K+GG YN  LQTSLP NLR YNP++ETA+SS   F +T PRGFA+E
Sbjct: 102 EIGKLGGGYNPLLQTSLPENLRGYNPDKETAESSHKAFTTTFPRGFALE 150


>ref|XP_007200367.1| hypothetical protein PRUPE_ppa010660mg [Prunus persica]
           gi|462395767|gb|EMJ01566.1| hypothetical protein
           PRUPE_ppa010660mg [Prunus persica]
          Length = 241

 Score =  152 bits (383), Expect = 1e-34
 Identities = 73/109 (66%), Positives = 85/109 (77%)
 Frame = -3

Query: 328 LFEEGRTNIWPEGSLEEKVQRLVKTWEMELVHKADPDQYKTLDAKKFKLGVNGRKFLTLE 149
           LFEEGRT IWP GSLE +VQ LVKTWEMEL HKA+ D +KTLD  K+   +NGRK +TLE
Sbjct: 42  LFEEGRTKIWPPGSLEHEVQNLVKTWEMELFHKANLDDFKTLDPNKYTFSLNGRKGITLE 101

Query: 148 EIIKVGGSYNVFLQTSLPANLRFYNPEEETADSSQTLFKSTLPRGFAVE 2
           EI K+GG YN  LQTSLP NLR YNP++ETA+SS   F +T PRGFA+E
Sbjct: 102 EIGKLGGGYNPLLQTSLPENLRGYNPDKETAESSHKAFTTTFPRGFALE 150