BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Forsythia23_contig00001013
         (2618 letters)

Database: ./nr 
           69,698,275 sequences; 24,982,196,650 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011092269.1| PREDICTED: isoamylase 2, chloroplastic [Sesa...  1135   0.0  
ref|XP_012842325.1| PREDICTED: isoamylase 2, chloroplastic [Eryt...  1046   0.0  
gb|EYU33286.1| hypothetical protein MIMGU_mgv1a001869mg [Erythra...  1046   0.0  
ref|XP_006355876.1| PREDICTED: isoamylase 2, chloroplastic [Sola...  1031   0.0  
ref|NP_001274804.1| isoamylase isoform 2 [Solanum tuberosum] gi|...  1030   0.0  
emb|CDP13520.1| unnamed protein product [Coffea canephora]           1024   0.0  
ref|XP_004247144.2| PREDICTED: isoamylase 2, chloroplastic [Sola...  1008   0.0  
ref|XP_009598489.1| PREDICTED: isoamylase 2, chloroplastic [Nico...  1008   0.0  
ref|XP_009761454.1| PREDICTED: isoamylase 2, chloroplastic [Nico...  1004   0.0  
ref|XP_007201750.1| hypothetical protein PRUPE_ppa001199mg [Prun...   996   0.0  
ref|XP_008234909.1| PREDICTED: isoamylase 2, chloroplastic [Prun...   996   0.0  
ref|XP_008386532.1| PREDICTED: isoamylase 2, chloroplastic-like ...   985   0.0  
ref|XP_009347699.1| PREDICTED: isoamylase 2, chloroplastic-like ...   984   0.0  
ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Viti...   981   0.0  
ref|XP_009356744.1| PREDICTED: isoamylase 2, chloroplastic-like ...   976   0.0  
ref|XP_007050677.1| Debranching enzyme 1 [Theobroma cacao] gi|50...   973   0.0  
ref|XP_010090367.1| Isoamylase 2 [Morus notabilis] gi|587849097|...   964   0.0  
ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|2...   962   0.0  
ref|XP_008368864.1| PREDICTED: isoamylase 2, chloroplastic-like ...   960   0.0  
ref|XP_012092290.1| PREDICTED: isoamylase 2, chloroplastic [Jatr...   959   0.0  

>ref|XP_011092269.1| PREDICTED: isoamylase 2, chloroplastic [Sesamum indicum]
            gi|747089281|ref|XP_011092270.1| PREDICTED: isoamylase 2,
            chloroplastic [Sesamum indicum]
          Length = 857

 Score = 1135 bits (2936), Expect = 0.0
 Identities = 554/741 (74%), Positives = 620/741 (83%), Gaps = 1/741 (0%)
 Frame = -2

Query: 2404 SSLHSGRDRDLVMSWGLFRSDSESFMPMDFQNSNADSKYSTIETPFVKDSSGRLVVELDF 2225
            S L+ G   +LVM WGLFR+DS +FMP+DFQ S+ADSKYSTIETPFVKDS+GRL VELDF
Sbjct: 119  SQLYEGHS-ELVMIWGLFRADSTNFMPLDFQRSSADSKYSTIETPFVKDSNGRLAVELDF 177

Query: 2224 DESLAPFYVSILLKS-LGSNLESSEIRSHRKTNFVVPVGFRLGRPAPLGLSVLANGSMNF 2048
            +ESLAPFYVSILLKS +GSN + S IRS RKTNF+VPVGF  G P+PLG+S   NG +NF
Sbjct: 178  EESLAPFYVSILLKSKIGSNSKISTIRSLRKTNFIVPVGFGSGYPSPLGISFSDNGFINF 237

Query: 2047 AFFSRHAESVVLCLYADTSANKPALEIDLDPYVNRSGDIWHALLDDSLPFVSYGYRCKSN 1868
            AFFSR AE VVLCLY D +  KPALEIDLDPYVNRSGDIWHA +D S+PFVSYGYRC+S 
Sbjct: 238  AFFSRDAERVVLCLYMDNTLEKPALEIDLDPYVNRSGDIWHASIDSSMPFVSYGYRCRSA 297

Query: 1867 ADKKRQYVLLDPYAKVIEDFGSGLPRKSVGKLWKEPGFDWSGDICPSLPMEKLIIYRLNV 1688
             D K  +VLLDPYAKVIED G GLPRK +GKL+KEP +DWSG+  PSLPMEKLIIYRLN+
Sbjct: 298  TDNKGHHVLLDPYAKVIEDLGLGLPRKCLGKLYKEPAYDWSGEFHPSLPMEKLIIYRLNI 357

Query: 1687 TRFTKDKSSKLPGDVVGTFAGISEKLQHFKDLGVNAILLEPIFPFDEQKGPYFPWHFFSP 1508
            T FTK+KSSKLPGD+ GTF+G+SEKL HFKDLGVNAILLEPIFPFDE+KGPYFPWHFFSP
Sbjct: 358  TGFTKNKSSKLPGDIAGTFSGLSEKLHHFKDLGVNAILLEPIFPFDEKKGPYFPWHFFSP 417

Query: 1507 GSQYGPSGDSEFVVKSMKEMVKKLHANRIEVLLEVVFTHTAEVAALRQIDNSSYYHVEGG 1328
                GP+GD   V +SMKEMVKKLH + IEVLLEVVFTHT E  ALR ID SSYYH EGG
Sbjct: 418  ERLCGPTGDPTTVAQSMKEMVKKLHGSGIEVLLEVVFTHTGEGCALRDID-SSYYHTEGG 476

Query: 1327 EDLESRNALNCNYPIVQQLILDSLRHWVIEYHIDGFCFINASSLMKGFHGEFLSRPPLVE 1148
            +DL SRNALNCNYPIVQQ++L+SLR+WVIE+HIDGFCFINAS LM+GFHGEFLSRPPLVE
Sbjct: 477  DDLTSRNALNCNYPIVQQMVLESLRYWVIEFHIDGFCFINASHLMRGFHGEFLSRPPLVE 536

Query: 1147 AIAFDPLLSKVKIIADSWDPHNIEKKDISFPHWKRWAEINSKFCNDVRNFLRGQGSLGNL 968
            AIAFDP+LSK KIIADSWDPH +E K+I FPHWKRWAE+N+KFC DVRNFLRGQG + NL
Sbjct: 537  AIAFDPVLSKAKIIADSWDPHEMETKEIVFPHWKRWAEMNAKFCVDVRNFLRGQGPISNL 596

Query: 967  ATRLCGSGDLFLDGRGPAFSFNFISRNAGLPLVDXXXXXXXXXXXXXSWNCGEEGPTNKT 788
            ATRLCGSGDLFL GRGPAFS NFI+RN GL LVD             SWNCGEEGPTNKT
Sbjct: 597  ATRLCGSGDLFLGGRGPAFSINFITRNVGLTLVDLVSFSSSTFGSELSWNCGEEGPTNKT 656

Query: 787  AVLERRLKQIRNYLLILFISLGVPILNMGDECGQSSGGSPAYADQKPLDWNALKSGFAIQ 608
             VLERRLKQIRN+L ILFISLGVP+LNMGDECGQS+GGS  YAD++PLDWNAL+SGF  Q
Sbjct: 657  LVLERRLKQIRNFLFILFISLGVPVLNMGDECGQSTGGSQEYADRRPLDWNALRSGFGTQ 716

Query: 607  TTKFISFLSSLKMRRSDLLQNRNFLREENIVWHGSDGSPPRWDGPSYRFLAMTLKADSEE 428
             T+FISFLS L+ RRSD+LQ R+FL+E+NI WHG+D +PP WD PS +FLAMTLKAD+  
Sbjct: 717  VTQFISFLSLLRRRRSDILQRRSFLKEDNIEWHGTDRTPPMWDDPSCKFLAMTLKADALP 776

Query: 427  SQSTSGSPEMIGDLFVAFNSAGHSENVALPPLTVDAAWFRLVDTSLPFPGFFTTDGVPLK 248
            SQ TSGSP   GDLFVAFNSA H + VALP L V + W RLVDT+LPFPGFFT DGVPL 
Sbjct: 777  SQVTSGSPNAGGDLFVAFNSANHLQKVALPQLAVGSTWVRLVDTALPFPGFFTVDGVPLD 836

Query: 247  DGSVAYEMKSHSCVLFEARSL 185
             G   YEMKSHSC+LFEAR L
Sbjct: 837  AGLATYEMKSHSCILFEARKL 857


>ref|XP_012842325.1| PREDICTED: isoamylase 2, chloroplastic [Erythranthe guttatus]
            gi|848884089|ref|XP_012842326.1| PREDICTED: isoamylase 2,
            chloroplastic [Erythranthe guttatus]
            gi|848884091|ref|XP_012842327.1| PREDICTED: isoamylase 2,
            chloroplastic [Erythranthe guttatus]
            gi|848884094|ref|XP_012842328.1| PREDICTED: isoamylase 2,
            chloroplastic [Erythranthe guttatus]
          Length = 840

 Score = 1046 bits (2704), Expect = 0.0
 Identities = 519/735 (70%), Positives = 605/735 (82%), Gaps = 4/735 (0%)
 Frame = -2

Query: 2380 RDLVMSWGLFRSDSESFMPMDFQNSNADSKYSTIETPFVKDSSGRLVVELDFDESLAPFY 2201
            R+L+M WG+FR D   FMP+  Q S+ D    T ETPFVKD   RL VEL+F+ESLAPFY
Sbjct: 128  RELIMIWGIFRDDLTKFMPV--QQSSTDG---TTETPFVKDCD-RLSVELEFEESLAPFY 181

Query: 2200 VSILLKS-LGSNLESSEIRSHRKTNFVVPVGFRLGRPAPLGLSVLANGSMNFAFFSRHAE 2024
            VSILLKS LGSN ++S IRSHRKTNF+VPVGFR GRP+PLG+S+L +G +NFAFFSR+A 
Sbjct: 182  VSILLKSKLGSNSKTSTIRSHRKTNFIVPVGFRSGRPSPLGISLLNDGFINFAFFSRNAY 241

Query: 2023 SVVLCLYADTSANKPALEIDLDPYVNRSGDIWHALLD-DSLPFVSYGYRCKSNA-DKKRQ 1850
            SVVLCLYADT+ +KPALEIDLDPYVNRSGDIWHA +D  SLPFVSYGYRC+S++ D K  
Sbjct: 242  SVVLCLYADTTVDKPALEIDLDPYVNRSGDIWHASIDCSSLPFVSYGYRCRSSSTDSKGH 301

Query: 1849 YVLLDPYAKVIEDFGSGLPRKSVGKLWKEPGFDWSGDICPSLPMEKLIIYRLNVTRFTKD 1670
             VL DPYAKVIE+FG   PRK +G+L  E  FDWSG+  P+LPMEKLI+YRLNV RFT D
Sbjct: 302  RVLPDPYAKVIEEFG---PRKCLGRLCNESAFDWSGESRPNLPMEKLIVYRLNVARFTND 358

Query: 1669 KSSKLPGDVVGTFAGISEKLQHFKDLGVNAILLEPIFPFDEQKGPYFPWHFFSPGSQYGP 1490
            KSSKLP D+ G+F+GIS+KL +FK+LGVNA+LLEPIFPFDEQKGPYFPWHFFSPGS YGP
Sbjct: 359  KSSKLPRDIAGSFSGISDKLHYFKNLGVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGP 418

Query: 1489 SGDSEFVVKSMKEMVKKLHANRIEVLLEVVFTHTAEVAALRQIDNSSYYHVEGGEDLESR 1310
            SGD   V  S+KEMVKKLHAN +EVLLEVVFTHT+E++ALR+IDNSSY+HVEGGE+L+SR
Sbjct: 419  SGDPSKVANSLKEMVKKLHANGMEVLLEVVFTHTSEISALREIDNSSYHHVEGGEELKSR 478

Query: 1309 NALNCNYPIVQQLILDSLRHWVIEYHIDGFCFINASSLMKGFHGEFLSRPPLVEAIAFDP 1130
            N+LNCNYPIVQQ+IL+ LR+WV E+HIDGFCFIN+SSL +GFHGEFLSRPPLVEAIAFDP
Sbjct: 479  NSLNCNYPIVQQMILECLRYWVTEFHIDGFCFINSSSLTRGFHGEFLSRPPLVEAIAFDP 538

Query: 1129 LLSKVKIIADSWDPHNIEKKDISFPHWKRWAEINSKFCNDVRNFLRGQGSLGNLATRLCG 950
            LLSKVKI+ADSWDPH +E K+I FPHWKRWAE+N+KFC+++RNF RG+GS+ +LATRLCG
Sbjct: 539  LLSKVKIVADSWDPHEMEMKEIVFPHWKRWAEMNAKFCSEMRNFFRGKGSISSLATRLCG 598

Query: 949  SGDLFLDGRGPAFSFNFISRNAGLPLVDXXXXXXXXXXXXXSWNCGEEGPTNKTAVLERR 770
            SGDLFL GR PAFSFNF++RN G  LVD             SWNCGEEGPTNK  VLE R
Sbjct: 599  SGDLFLGGRSPAFSFNFVTRNVGPTLVD----LVSYNSIESSWNCGEEGPTNKILVLETR 654

Query: 769  LKQIRNYLLILFISLGVPILNMGDECGQSSGGSPAYADQKPLDWNALKSGFAIQTTKFIS 590
            LKQIRN++ +LF+SLGVP+LNMGDECGQS+ GSP Y+D+   DW+ALKSGF  Q T+FIS
Sbjct: 655  LKQIRNFIFVLFVSLGVPVLNMGDECGQSTRGSPEYSDRISFDWSALKSGFGTQVTEFIS 714

Query: 589  FLSSLKMRRSDLLQNRNFLREENIVWHGSDGSPPRWDGPSYRFLAMTLKADSEESQSTSG 410
            FLSSL++RR+DLLQ R+FL+E+NI WHG++G PP WD PS +FLAMTLKAD         
Sbjct: 715  FLSSLRIRRNDLLQKRSFLKEDNIEWHGAEGGPPMWDDPSCKFLAMTLKADPP------- 767

Query: 409  SPEMIGDLFVAFNSAGHSENVALPPL-TVDAAWFRLVDTSLPFPGFFTTDGVPLKDGSVA 233
                + DLFVAFN    SE VALPPL  VD  W  LVDTSLPFPGFFT +GVPL+DG   
Sbjct: 768  ----LSDLFVAFNGGDCSEKVALPPLAAVDTNWVCLVDTSLPFPGFFTEEGVPLEDGLET 823

Query: 232  YEMKSHSCVLFEARS 188
            YEMKSHSCVLFEARS
Sbjct: 824  YEMKSHSCVLFEARS 838


>gb|EYU33286.1| hypothetical protein MIMGU_mgv1a001869mg [Erythranthe guttata]
          Length = 747

 Score = 1046 bits (2704), Expect = 0.0
 Identities = 519/735 (70%), Positives = 605/735 (82%), Gaps = 4/735 (0%)
 Frame = -2

Query: 2380 RDLVMSWGLFRSDSESFMPMDFQNSNADSKYSTIETPFVKDSSGRLVVELDFDESLAPFY 2201
            R+L+M WG+FR D   FMP+  Q S+ D    T ETPFVKD   RL VEL+F+ESLAPFY
Sbjct: 35   RELIMIWGIFRDDLTKFMPV--QQSSTDG---TTETPFVKDCD-RLSVELEFEESLAPFY 88

Query: 2200 VSILLKS-LGSNLESSEIRSHRKTNFVVPVGFRLGRPAPLGLSVLANGSMNFAFFSRHAE 2024
            VSILLKS LGSN ++S IRSHRKTNF+VPVGFR GRP+PLG+S+L +G +NFAFFSR+A 
Sbjct: 89   VSILLKSKLGSNSKTSTIRSHRKTNFIVPVGFRSGRPSPLGISLLNDGFINFAFFSRNAY 148

Query: 2023 SVVLCLYADTSANKPALEIDLDPYVNRSGDIWHALLD-DSLPFVSYGYRCKSNA-DKKRQ 1850
            SVVLCLYADT+ +KPALEIDLDPYVNRSGDIWHA +D  SLPFVSYGYRC+S++ D K  
Sbjct: 149  SVVLCLYADTTVDKPALEIDLDPYVNRSGDIWHASIDCSSLPFVSYGYRCRSSSTDSKGH 208

Query: 1849 YVLLDPYAKVIEDFGSGLPRKSVGKLWKEPGFDWSGDICPSLPMEKLIIYRLNVTRFTKD 1670
             VL DPYAKVIE+FG   PRK +G+L  E  FDWSG+  P+LPMEKLI+YRLNV RFT D
Sbjct: 209  RVLPDPYAKVIEEFG---PRKCLGRLCNESAFDWSGESRPNLPMEKLIVYRLNVARFTND 265

Query: 1669 KSSKLPGDVVGTFAGISEKLQHFKDLGVNAILLEPIFPFDEQKGPYFPWHFFSPGSQYGP 1490
            KSSKLP D+ G+F+GIS+KL +FK+LGVNA+LLEPIFPFDEQKGPYFPWHFFSPGS YGP
Sbjct: 266  KSSKLPRDIAGSFSGISDKLHYFKNLGVNAVLLEPIFPFDEQKGPYFPWHFFSPGSLYGP 325

Query: 1489 SGDSEFVVKSMKEMVKKLHANRIEVLLEVVFTHTAEVAALRQIDNSSYYHVEGGEDLESR 1310
            SGD   V  S+KEMVKKLHAN +EVLLEVVFTHT+E++ALR+IDNSSY+HVEGGE+L+SR
Sbjct: 326  SGDPSKVANSLKEMVKKLHANGMEVLLEVVFTHTSEISALREIDNSSYHHVEGGEELKSR 385

Query: 1309 NALNCNYPIVQQLILDSLRHWVIEYHIDGFCFINASSLMKGFHGEFLSRPPLVEAIAFDP 1130
            N+LNCNYPIVQQ+IL+ LR+WV E+HIDGFCFIN+SSL +GFHGEFLSRPPLVEAIAFDP
Sbjct: 386  NSLNCNYPIVQQMILECLRYWVTEFHIDGFCFINSSSLTRGFHGEFLSRPPLVEAIAFDP 445

Query: 1129 LLSKVKIIADSWDPHNIEKKDISFPHWKRWAEINSKFCNDVRNFLRGQGSLGNLATRLCG 950
            LLSKVKI+ADSWDPH +E K+I FPHWKRWAE+N+KFC+++RNF RG+GS+ +LATRLCG
Sbjct: 446  LLSKVKIVADSWDPHEMEMKEIVFPHWKRWAEMNAKFCSEMRNFFRGKGSISSLATRLCG 505

Query: 949  SGDLFLDGRGPAFSFNFISRNAGLPLVDXXXXXXXXXXXXXSWNCGEEGPTNKTAVLERR 770
            SGDLFL GR PAFSFNF++RN G  LVD             SWNCGEEGPTNK  VLE R
Sbjct: 506  SGDLFLGGRSPAFSFNFVTRNVGPTLVD----LVSYNSIESSWNCGEEGPTNKILVLETR 561

Query: 769  LKQIRNYLLILFISLGVPILNMGDECGQSSGGSPAYADQKPLDWNALKSGFAIQTTKFIS 590
            LKQIRN++ +LF+SLGVP+LNMGDECGQS+ GSP Y+D+   DW+ALKSGF  Q T+FIS
Sbjct: 562  LKQIRNFIFVLFVSLGVPVLNMGDECGQSTRGSPEYSDRISFDWSALKSGFGTQVTEFIS 621

Query: 589  FLSSLKMRRSDLLQNRNFLREENIVWHGSDGSPPRWDGPSYRFLAMTLKADSEESQSTSG 410
            FLSSL++RR+DLLQ R+FL+E+NI WHG++G PP WD PS +FLAMTLKAD         
Sbjct: 622  FLSSLRIRRNDLLQKRSFLKEDNIEWHGAEGGPPMWDDPSCKFLAMTLKADPP------- 674

Query: 409  SPEMIGDLFVAFNSAGHSENVALPPL-TVDAAWFRLVDTSLPFPGFFTTDGVPLKDGSVA 233
                + DLFVAFN    SE VALPPL  VD  W  LVDTSLPFPGFFT +GVPL+DG   
Sbjct: 675  ----LSDLFVAFNGGDCSEKVALPPLAAVDTNWVCLVDTSLPFPGFFTEEGVPLEDGLET 730

Query: 232  YEMKSHSCVLFEARS 188
            YEMKSHSCVLFEARS
Sbjct: 731  YEMKSHSCVLFEARS 745


>ref|XP_006355876.1| PREDICTED: isoamylase 2, chloroplastic [Solanum tuberosum]
          Length = 878

 Score = 1031 bits (2667), Expect = 0.0
 Identities = 510/747 (68%), Positives = 592/747 (79%), Gaps = 6/747 (0%)
 Frame = -2

Query: 2407 LSSLHSGRDRDLVMSWGLFRSDSESFMPMDFQNSNADSKYSTIETPFVKDSSGRLVVELD 2228
            LS  HS    +LVM WGLFRSD+  FMP+D     AD K ST+ETPFV+  SG++ VELD
Sbjct: 120  LSYAHS----ELVMVWGLFRSDASCFMPLDLNRRGADGKSSTVETPFVQGPSGKVTVELD 175

Query: 2227 FDESLAPFYVSILLKS-LGSNLESSEIRSHRKTNFVVPVGFRLGRPAPLGLSVLANGSMN 2051
            F+ SLAPFY+S  +KS L S++E+SEIRSHR TNFVVPVG   G PAPLG+S   +GS+N
Sbjct: 176  FEASLAPFYISFYMKSQLVSDMENSEIRSHRNTNFVVPVGLSSGHPAPLGISFQPDGSVN 235

Query: 2050 FAFFSRHAESVVLCLYADTSANKPALEIDLDPYVNRSGDIWHALLDDSLPFVSYGYRCKS 1871
            FA FSR A SVVLCLY D S  KP+LEIDLDPY+NRSGDIWHA LD SLPF +YGYRCK+
Sbjct: 236  FALFSRSARSVVLCLYDDISVEKPSLEIDLDPYINRSGDIWHAALDCSLPFKTYGYRCKA 295

Query: 1870 NADKKRQYVLLDPYAKVIEDF-----GSGLPRKSVGKLWKEPGFDWSGDICPSLPMEKLI 1706
                K + VLLDPYAKVI        GS +  K +G+L  EPG+DWSGD+ PSLPMEKLI
Sbjct: 296  TTSGKGELVLLDPYAKVIRRVIPRQGGSEIRPKYLGELCLEPGYDWSGDVPPSLPMEKLI 355

Query: 1705 IYRLNVTRFTKDKSSKLPGDVVGTFAGISEKLQHFKDLGVNAILLEPIFPFDEQKGPYFP 1526
            IYRLNVT+FTKDKSSKLP D+ GTF+GISEK  HFKDLGVNA+LLEPIFPFDEQKGPYFP
Sbjct: 356  IYRLNVTQFTKDKSSKLPDDLAGTFSGISEKWHHFKDLGVNAMLLEPIFPFDEQKGPYFP 415

Query: 1525 WHFFSPGSQYGPSGDSEFVVKSMKEMVKKLHANRIEVLLEVVFTHTAEVAALRQIDNSSY 1346
            WHFFSPG+ YGPSGD    +KSMK+MVKKLHAN IEV LEVVFTHTAE A L  +DN SY
Sbjct: 416  WHFFSPGNMYGPSGDPLSAIKSMKDMVKKLHANGIEVFLEVVFTHTAEDAPLMNVDNFSY 475

Query: 1345 YHVEGGEDLESRNALNCNYPIVQQLILDSLRHWVIEYHIDGFCFINASSLMKGFHGEFLS 1166
              ++GG+ L  +NALNCNYPIVQQ+ILD LRHWVIE+HIDGF F+NASSL++GF+GE LS
Sbjct: 476  C-IKGGQYLNIQNALNCNYPIVQQMILDCLRHWVIEFHIDGFVFVNASSLLRGFNGEILS 534

Query: 1165 RPPLVEAIAFDPLLSKVKIIADSWDPHNIEKKDISFPHWKRWAEINSKFCNDVRNFLRGQ 986
            RPPLVEAIAFDP+LSKVK+IAD+W+P   + K+  FPHW+RWAEIN +FC+D+R+FLRG+
Sbjct: 535  RPPLVEAIAFDPILSKVKMIADNWNPLTNDSKENLFPHWRRWAEINMRFCDDIRDFLRGE 594

Query: 985  GSLGNLATRLCGSGDLFLDGRGPAFSFNFISRNAGLPLVDXXXXXXXXXXXXXSWNCGEE 806
            G L NLATRLCGSGD+F  GRGPAFSFN+I+RN+GL LVD             SWNCG+E
Sbjct: 595  GLLSNLATRLCGSGDIFAGGRGPAFSFNYIARNSGLTLVDLVSFSSNEVASELSWNCGQE 654

Query: 805  GPTNKTAVLERRLKQIRNYLLILFISLGVPILNMGDECGQSSGGSPAYADQKPLDWNALK 626
            G T    VLERRLKQ+RN+L ILFISLGVP+LNMGDECGQSSGGSPAY  +K L WN LK
Sbjct: 655  GATTNNIVLERRLKQVRNFLFILFISLGVPVLNMGDECGQSSGGSPAYDARKSLGWNTLK 714

Query: 625  SGFAIQTTKFISFLSSLKMRRSDLLQNRNFLREENIVWHGSDGSPPRWDGPSYRFLAMTL 446
            +GF  Q  +FISFLS+L+MRRSDLLQ R FL+EENI WHGSD SPP+WDGPS +FLAMTL
Sbjct: 715  TGFGTQIAQFISFLSNLRMRRSDLLQKRTFLKEENIQWHGSDQSPPKWDGPSSKFLAMTL 774

Query: 445  KADSEESQSTSGSPEMIGDLFVAFNSAGHSENVALPPLTVDAAWFRLVDTSLPFPGFFTT 266
            KAD+E SQ+     +++GDLFVAFN AG SE V LPP   D  W RLVDT+LPFPGFF  
Sbjct: 775  KADAEVSQTLVS--DIVGDLFVAFNGAGDSEIVILPPPPTDMVWHRLVDTALPFPGFFDE 832

Query: 265  DGVPLKDGSVAYEMKSHSCVLFEARSL 185
             G P++D  VAYEMKSHSC+LFEA+ L
Sbjct: 833  KGTPVEDELVAYEMKSHSCLLFEAQRL 859


>ref|NP_001274804.1| isoamylase isoform 2 [Solanum tuberosum] gi|27728147|gb|AAN15318.1|
            isoamylase isoform 2 [Solanum tuberosum]
          Length = 878

 Score = 1030 bits (2662), Expect = 0.0
 Identities = 509/747 (68%), Positives = 591/747 (79%), Gaps = 6/747 (0%)
 Frame = -2

Query: 2407 LSSLHSGRDRDLVMSWGLFRSDSESFMPMDFQNSNADSKYSTIETPFVKDSSGRLVVELD 2228
            LS  HS    +LVM WGLFRSD+  FMP+D     AD K ST+ETPFV+  SG++ VELD
Sbjct: 120  LSYAHS----ELVMVWGLFRSDASCFMPLDLNRRGADGKSSTVETPFVQGPSGKVTVELD 175

Query: 2227 FDESLAPFYVSILLKS-LGSNLESSEIRSHRKTNFVVPVGFRLGRPAPLGLSVLANGSMN 2051
            F+ SLAPFY+S  +KS L S++E+SEIRSHR TNFVVPVG   G PAPLG+S   +GS+N
Sbjct: 176  FEASLAPFYISFYMKSQLVSDMENSEIRSHRNTNFVVPVGLSSGHPAPLGISFQPDGSVN 235

Query: 2050 FAFFSRHAESVVLCLYADTSANKPALEIDLDPYVNRSGDIWHALLDDSLPFVSYGYRCKS 1871
            FA FSR A SVVLCLY D S  KP+LEIDLDPY+NRSGDIWHA LD SLPF +YGYRCK+
Sbjct: 236  FALFSRSARSVVLCLYDDISVEKPSLEIDLDPYINRSGDIWHAALDCSLPFKTYGYRCKA 295

Query: 1870 NADKKRQYVLLDPYAKVIEDF-----GSGLPRKSVGKLWKEPGFDWSGDICPSLPMEKLI 1706
                K + VLLDPYAKVI        GS +  K +G+L  EPG+DWSGD+ PSLPMEKLI
Sbjct: 296  TTSGKGELVLLDPYAKVIRRVIPRQGGSEIRPKYLGELCLEPGYDWSGDVPPSLPMEKLI 355

Query: 1705 IYRLNVTRFTKDKSSKLPGDVVGTFAGISEKLQHFKDLGVNAILLEPIFPFDEQKGPYFP 1526
            IYRLNVT+FTKDKSSKLP D+ GTF+GISEK  HFKDLGVNA+LLEPIFPFDEQKGPYFP
Sbjct: 356  IYRLNVTQFTKDKSSKLPDDLAGTFSGISEKWHHFKDLGVNAMLLEPIFPFDEQKGPYFP 415

Query: 1525 WHFFSPGSQYGPSGDSEFVVKSMKEMVKKLHANRIEVLLEVVFTHTAEVAALRQIDNSSY 1346
            WHFFSPG+ YGPSGD    +KSMK+MVKKLHAN IEV LEVVFTHTAE A L  +DN SY
Sbjct: 416  WHFFSPGNMYGPSGDPLSAIKSMKDMVKKLHANGIEVFLEVVFTHTAEDAPLMNVDNFSY 475

Query: 1345 YHVEGGEDLESRNALNCNYPIVQQLILDSLRHWVIEYHIDGFCFINASSLMKGFHGEFLS 1166
              ++GG+ L  +NALNCNYPIVQQ+ILD LRHWVIE+HIDGF F+NASSL++GF+GE LS
Sbjct: 476  C-IKGGQYLNIQNALNCNYPIVQQMILDCLRHWVIEFHIDGFVFVNASSLLRGFNGEILS 534

Query: 1165 RPPLVEAIAFDPLLSKVKIIADSWDPHNIEKKDISFPHWKRWAEINSKFCNDVRNFLRGQ 986
            RPPLVEAIAFDP+LSKVK+IAD+W+P   + K+  FPHW+RWAEIN +FC+D+R+FLRG+
Sbjct: 535  RPPLVEAIAFDPILSKVKMIADNWNPLTNDSKENLFPHWRRWAEINMRFCDDIRDFLRGE 594

Query: 985  GSLGNLATRLCGSGDLFLDGRGPAFSFNFISRNAGLPLVDXXXXXXXXXXXXXSWNCGEE 806
            G L NLATRLCGSGD+F  GRGPAFSFN+I+RN+GL LVD             SWNCG+E
Sbjct: 595  GLLSNLATRLCGSGDIFAGGRGPAFSFNYIARNSGLTLVDLVSFSSNEVASELSWNCGQE 654

Query: 805  GPTNKTAVLERRLKQIRNYLLILFISLGVPILNMGDECGQSSGGSPAYADQKPLDWNALK 626
            G T    VLERRLKQ+RN+L ILFISLGVP+LNMGDECGQSSGG PAY  +K L WN LK
Sbjct: 655  GATTNNIVLERRLKQVRNFLFILFISLGVPVLNMGDECGQSSGGPPAYDARKSLGWNTLK 714

Query: 625  SGFAIQTTKFISFLSSLKMRRSDLLQNRNFLREENIVWHGSDGSPPRWDGPSYRFLAMTL 446
            +GF  Q  +FISFLS+L+MRRSDLLQ R FL+EENI WHGSD SPP+WDGPS +FLAMTL
Sbjct: 715  TGFGTQIAQFISFLSNLRMRRSDLLQKRTFLKEENIQWHGSDQSPPKWDGPSSKFLAMTL 774

Query: 445  KADSEESQSTSGSPEMIGDLFVAFNSAGHSENVALPPLTVDAAWFRLVDTSLPFPGFFTT 266
            KAD+E SQ+     +++GDLFVAFN AG SE V LPP   D  W RLVDT+LPFPGFF  
Sbjct: 775  KADAEVSQTLVS--DIVGDLFVAFNGAGDSEIVILPPPPTDMVWHRLVDTALPFPGFFDE 832

Query: 265  DGVPLKDGSVAYEMKSHSCVLFEARSL 185
             G P++D  VAYEMKSHSC+LFEA+ L
Sbjct: 833  KGTPVEDELVAYEMKSHSCLLFEAQRL 859


>emb|CDP13520.1| unnamed protein product [Coffea canephora]
          Length = 871

 Score = 1024 bits (2647), Expect = 0.0
 Identities = 501/737 (67%), Positives = 585/737 (79%), Gaps = 5/737 (0%)
 Frame = -2

Query: 2386 RDRDLVMSWGLFRSDSESFMPMDFQNSNADSKYSTIETPFVKDSSGRLVVELDFDESLAP 2207
            R+ +LVMSWGLFRS S SFMP+DFQ S  D K  T+ETPF+++S G L VELDF+ +LAP
Sbjct: 132  RENELVMSWGLFRSHSSSFMPLDFQGSTLDGKTITMETPFMQESEGTLAVELDFELTLAP 191

Query: 2206 FYVSILLKS-LGSNLESSEIRSHRKTNFVVPVGFRLGRPAPLGLSVLANGSMNFAFFSRH 2030
            FY S LL+S L S + S EIRSHRKT+FVVPVGF  G P+PLGLS  A+GS+NFA FSR 
Sbjct: 192  FYFSFLLRSQLDSGMSSLEIRSHRKTSFVVPVGFGSGNPSPLGLSFSADGSLNFALFSRT 251

Query: 2029 AESVVLCLYADTSANKPALEIDLDPYVNRSGDIWHALLDDSLPFVSYGYRCKSNADKKRQ 1850
            AESVVLCLY   + ++P LEIDLDPYVN+SGDIWHA +D S  F SYGYRCK   D +++
Sbjct: 252  AESVVLCLYDGKTTHRPNLEIDLDPYVNKSGDIWHASIDRSFQFASYGYRCKVAEDAEQE 311

Query: 1849 YVLLDPYAKVIEDFGSGLPRKSV----GKLWKEPGFDWSGDICPSLPMEKLIIYRLNVTR 1682
            +VLLDPYAK+I D  +G    S+    G+L K P FDW   + P L +E+L++YRLNV R
Sbjct: 312  HVLLDPYAKLIGDVPAGSQSTSLLTCLGQLSKVPPFDWGQQMRPCLRLEELVVYRLNVMR 371

Query: 1681 FTKDKSSKLPGDVVGTFAGISEKLQHFKDLGVNAILLEPIFPFDEQKGPYFPWHFFSPGS 1502
            FTKDKSS LP ++ G+F G++EKL HFKDLGVNAILLEP+FPFDEQKGPYFPWHFFSP +
Sbjct: 372  FTKDKSSNLPNNLGGSFLGVTEKLHHFKDLGVNAILLEPVFPFDEQKGPYFPWHFFSPAN 431

Query: 1501 QYGPSGDSEFVVKSMKEMVKKLHANRIEVLLEVVFTHTAEVAALRQIDNSSYYHVEGGED 1322
            QYG  GD    + +MKEMVKKLH N IEVLLEV FTH AEV ALR IDN+SY HV+  +D
Sbjct: 432  QYGSPGDPVSCINTMKEMVKKLHNNGIEVLLEVDFTHAAEVGALRIIDNTSYCHVKTVDD 491

Query: 1321 LESRNALNCNYPIVQQLILDSLRHWVIEYHIDGFCFINASSLMKGFHGEFLSRPPLVEAI 1142
              S +ALNCNYP+V QLILD LRHWVIE+HIDGFCF+NASSL++GFHGE+LSRPPLVEAI
Sbjct: 492  TGSEHALNCNYPVVAQLILDCLRHWVIEFHIDGFCFVNASSLLRGFHGEYLSRPPLVEAI 551

Query: 1141 AFDPLLSKVKIIADSWDPHNIEKKDISFPHWKRWAEINSKFCNDVRNFLRGQGSLGNLAT 962
            AFDPLLSKVKIIADSWDP  ++ K++ FPHWK+WAEIN+KFC D+RNFLRG+G L +LAT
Sbjct: 552  AFDPLLSKVKIIADSWDPREMKVKEVLFPHWKKWAEINNKFCYDIRNFLRGEGLLSDLAT 611

Query: 961  RLCGSGDLFLDGRGPAFSFNFISRNAGLPLVDXXXXXXXXXXXXXSWNCGEEGPTNKTAV 782
            R+CGSGD+FLDGRGPAFSFNFI+RN GL LVD             SWNCGEEG TNK  V
Sbjct: 612  RICGSGDVFLDGRGPAFSFNFIARNFGLSLVDLVSFSSSKLAKEFSWNCGEEGATNKNDV 671

Query: 781  LERRLKQIRNYLLILFISLGVPILNMGDECGQSSGGSPAYADQKPLDWNALKSGFAIQTT 602
            LERRLKQIRN+L ILFISLGVP+LNMGDECGQSSGGSPAY D+   DWNAL SGF+IQT 
Sbjct: 672  LERRLKQIRNFLFILFISLGVPVLNMGDECGQSSGGSPAYGDRNSFDWNALSSGFSIQTV 731

Query: 601  KFISFLSSLKMRRSDLLQNRNFLREENIVWHGSDGSPPRWDGPSYRFLAMTLKADSEESQ 422
            +FISFL+SL++RRSDLLQ RNFLREE+I WHGS+ +PPRWD  + RFLAMTLKA SE+ +
Sbjct: 732  QFISFLTSLRIRRSDLLQKRNFLREESIEWHGSNQAPPRWDDAASRFLAMTLKASSEDIE 791

Query: 421  STSGSPEMIGDLFVAFNSAGHSENVALPPLTVDAAWFRLVDTSLPFPGFFTTDGVPLKDG 242
            S S  P   GDLF AFN A  SE++ LPP   D  WFRLVDT+LPFPGFFT +G  ++DG
Sbjct: 792  SNS-VPNACGDLFAAFNGADLSESITLPPPPADMVWFRLVDTALPFPGFFTANGACIEDG 850

Query: 241  SVAYEMKSHSCVLFEAR 191
               YEMKSHSC LFEAR
Sbjct: 851  LATYEMKSHSCALFEAR 867


>ref|XP_004247144.2| PREDICTED: isoamylase 2, chloroplastic [Solanum lycopersicum]
          Length = 866

 Score = 1008 bits (2606), Expect = 0.0
 Identities = 497/747 (66%), Positives = 587/747 (78%), Gaps = 6/747 (0%)
 Frame = -2

Query: 2407 LSSLHSGRDRDLVMSWGLFRSDSESFMPMDFQNSNADSKYSTIETPFVKDSSGRLVVELD 2228
            LS  HS    +LVM WGLFRSD+  FMP+D     AD K ST+ETPFV+  SG++ VELD
Sbjct: 120  LSDAHS----ELVMVWGLFRSDASCFMPLDLNRRGADGKSSTVETPFVQGPSGKVTVELD 175

Query: 2227 FDESLAPFYVSILLKS-LGSNLESSEIRSHRKTNFVVPVGFRLGRPAPLGLSVLANGSMN 2051
            F+ SL PFY+S  +KS L S++E+SEIRSHR TNFVVPVG   G PAPLG+S   +G +N
Sbjct: 176  FEVSLVPFYISFYMKSQLVSDMENSEIRSHRNTNFVVPVGLSSGHPAPLGISFQPDGYVN 235

Query: 2050 FAFFSRHAESVVLCLYADTSANKPALEIDLDPYVNRSGDIWHALLDDSLPFVSYGYRCKS 1871
            FA +SR A+ VVLCLY D S  KP+LEIDLDPY+N+SGDIWHA LD SLPF +YGYR K+
Sbjct: 236  FALYSRSAKGVVLCLYDDISVEKPSLEIDLDPYINQSGDIWHAALDCSLPFKTYGYRFKA 295

Query: 1870 NADKKRQYVLLDPYAKVIEDF-----GSGLPRKSVGKLWKEPGFDWSGDICPSLPMEKLI 1706
                K + VLLDPYAKVI        GS +  K +G+L  EPG+DWSGD+ PSLPMEKLI
Sbjct: 296  ATSGKGELVLLDPYAKVIRSVIPRQGGSEIRPKYLGELCLEPGYDWSGDVPPSLPMEKLI 355

Query: 1705 IYRLNVTRFTKDKSSKLPGDVVGTFAGISEKLQHFKDLGVNAILLEPIFPFDEQKGPYFP 1526
            IYR+NVT FTKDKSSKLP ++ GTF+GISEK  H KDLGVNA+LLEPIFPFDEQKGPY+P
Sbjct: 356  IYRINVTHFTKDKSSKLPDNLAGTFSGISEKWHHLKDLGVNAMLLEPIFPFDEQKGPYYP 415

Query: 1525 WHFFSPGSQYGPSGDSEFVVKSMKEMVKKLHANRIEVLLEVVFTHTAEVAALRQIDNSSY 1346
            WHFFSPG+ YGPSGD   V+KSMK+MVKKLHAN IEV LEVVFTHTAE A L  +DN SY
Sbjct: 416  WHFFSPGNMYGPSGDPLSVIKSMKDMVKKLHANGIEVFLEVVFTHTAEDAPLMNVDNFSY 475

Query: 1345 YHVEGGEDLESRNALNCNYPIVQQLILDSLRHWVIEYHIDGFCFINASSLMKGFHGEFLS 1166
              ++G +D+  +NALNCNYPIVQQ+ILD LRHWVIE+HIDGF F++ASSL++GF+GE LS
Sbjct: 476  C-IKGSQDMNIQNALNCNYPIVQQMILDCLRHWVIEFHIDGFVFVDASSLLRGFNGEILS 534

Query: 1165 RPPLVEAIAFDPLLSKVKIIADSWDPHNIEKKDISFPHWKRWAEINSKFCNDVRNFLRGQ 986
            RPPLVEAIAFDP+LSK K+IAD+W+P   +  +  FPHW+RWAEIN +FC+D+R+FLRG+
Sbjct: 535  RPPLVEAIAFDPILSKAKMIADNWNPLTNDSTENLFPHWRRWAEINMRFCDDIRDFLRGE 594

Query: 985  GSLGNLATRLCGSGDLFLDGRGPAFSFNFISRNAGLPLVDXXXXXXXXXXXXXSWNCGEE 806
            G L NLATRLCGSGD+F  GRGPAFSFN+I+RN+GL LVD             SWNCG+E
Sbjct: 595  GLLSNLATRLCGSGDIFAGGRGPAFSFNYIARNSGLTLVDLVSFSNSEVASELSWNCGQE 654

Query: 805  GPTNKTAVLERRLKQIRNYLLILFISLGVPILNMGDECGQSSGGSPAYADQKPLDWNALK 626
            G T  + VLERRLKQ+RN+L ILFISLGVP+LNMGDECGQSSGGSPAY  +K L+WN LK
Sbjct: 655  GATTNSIVLERRLKQVRNFLFILFISLGVPVLNMGDECGQSSGGSPAYDARKSLNWNTLK 714

Query: 625  SGFAIQTTKFISFLSSLKMRRSDLLQNRNFLREENIVWHGSDGSPPRWDGPSYRFLAMTL 446
            +GF  Q  +FISFLS+L+MRRSDLLQ RNFL+EENI WHGSD SPP WD PS +FLAMTL
Sbjct: 715  TGFGTQIAQFISFLSNLRMRRSDLLQKRNFLKEENIQWHGSDQSPPVWDDPSSKFLAMTL 774

Query: 445  KADSEESQSTSGSPEMIGDLFVAFNSAGHSENVALPPLTVDAAWFRLVDTSLPFPGFFTT 266
            KAD+E S +     ++ GDLFVAFN AG SE+V LPP   D  W+RLVDT+LPFPGFF  
Sbjct: 775  KADAEVSHTLLS--DIGGDLFVAFNGAGDSESVILPPPPTDMVWYRLVDTALPFPGFFDE 832

Query: 265  DGVPLKDGSVAYEMKSHSCVLFEARSL 185
             G P++D  VAYEMKSHSCVLFEA+ L
Sbjct: 833  KGTPVEDELVAYEMKSHSCVLFEAQRL 859


>ref|XP_009598489.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana tomentosiformis]
          Length = 863

 Score = 1008 bits (2605), Expect = 0.0
 Identities = 506/747 (67%), Positives = 585/747 (78%), Gaps = 6/747 (0%)
 Frame = -2

Query: 2407 LSSLHSGRDRDLVMSWGLFRSDSESFMPMDFQNSNADSKYSTIETPFVKDSSGRLVVELD 2228
            LS  H+    +LVM WGLFRSD+  FMP+D     AD K STIETPFV+  S ++ VELD
Sbjct: 121  LSDAHA----ELVMVWGLFRSDASCFMPLDLNRHGADGKSSTIETPFVQGPSDKVTVELD 176

Query: 2227 FDESLAPFYVSILLKS-LGSNLESSEIRSHRKTNFVVPVGFRLGRPAPLGLSVLANGSMN 2051
            F+ SLAPFY+S  +KS L S+++SSEIRSHR T FVVPVG   G PAPLGLS   +G+MN
Sbjct: 177  FEASLAPFYISFYMKSQLVSDMKSSEIRSHRNTTFVVPVGLTSGHPAPLGLSCQPDGTMN 236

Query: 2050 FAFFSRHAESVVLCLYADTSANKPALEIDLDPYVNRSGDIWHALLDDSLPFVSYGYRCKS 1871
            FA FSR A+SVVLCLY D S  KP+LEIDLDPYVNRSGDIWHA LD SLP  +YGYRCK 
Sbjct: 237  FALFSRSAKSVVLCLYDDISVEKPSLEIDLDPYVNRSGDIWHAALDCSLPIKTYGYRCKV 296

Query: 1870 NADKKRQYVLLDPYAKVIEDF-----GSGLPRKSVGKLWKEPGFDWSGDICPSLPMEKLI 1706
                K + VLLDPY+KVI        GS L  K +G+L KEPG+DWS D+ PSL MEKLI
Sbjct: 297  ATSGKEELVLLDPYSKVIRSVIPRQGGSKLRPKYLGELCKEPGYDWSVDVPPSLHMEKLI 356

Query: 1705 IYRLNVTRFTKDKSSKLPGDVVGTFAGISEKLQHFKDLGVNAILLEPIFPFDEQKGPYFP 1526
            IYRLNVT FTKD SSKLP D+ GTF+GI+EK  HFK LGVNAILLEPIFPF+EQKGPYFP
Sbjct: 357  IYRLNVTEFTKDGSSKLPDDLAGTFSGITEKWSHFKHLGVNAILLEPIFPFEEQKGPYFP 416

Query: 1525 WHFFSPGSQYGPSGDSEFVVKSMKEMVKKLHANRIEVLLEVVFTHTAEVAALRQIDNSSY 1346
             HFFSPG+ +G SGD   V+KSMK+MVKKLHAN IE+ LEVVFTHTAE A L  +DNSSY
Sbjct: 417  SHFFSPGNMHGRSGDPLSVIKSMKDMVKKLHANGIEIFLEVVFTHTAEDAPLMHVDNSSY 476

Query: 1345 YHVEGGEDLESRNALNCNYPIVQQLILDSLRHWVIEYHIDGFCFINASSLMKGFHGEFLS 1166
            Y ++GG DL  + ALNCNYPIVQQ+ILD LR+WVIE+HIDGF FINASSL+KGFHGE  S
Sbjct: 477  YSIKGG-DLNIQYALNCNYPIVQQMILDCLRYWVIEFHIDGFVFINASSLLKGFHGEIQS 535

Query: 1165 RPPLVEAIAFDPLLSKVKIIADSWDPHNIEKKDISFPHWKRWAEINSKFCNDVRNFLRGQ 986
            RPPLVEAIAFDP+LSKVKIIAD WD    + K+I FPHW++WAEIN++FC+D+R+FLRG+
Sbjct: 536  RPPLVEAIAFDPILSKVKIIADYWDALANDSKEILFPHWRKWAEINTRFCDDIRDFLRGK 595

Query: 985  GSLGNLATRLCGSGDLFLDGRGPAFSFNFISRNAGLPLVDXXXXXXXXXXXXXSWNCGEE 806
            G L +LATRLCGSGD+F  GRGPAFSFN+I+RN GL LVD             SWNCGEE
Sbjct: 596  GLLSSLATRLCGSGDIFSGGRGPAFSFNYIARNFGLTLVDLVSFSSSEVASELSWNCGEE 655

Query: 805  GPTNKTAVLERRLKQIRNYLLILFISLGVPILNMGDECGQSSGGSPAYADQKPLDWNALK 626
            GPT    VLE RLKQ+RN+L ILFISLGVP+LNMGDECGQSS GSP +  +K  DW+ LK
Sbjct: 656  GPTTNNIVLETRLKQVRNFLFILFISLGVPVLNMGDECGQSSRGSPVHGARKSFDWSTLK 715

Query: 625  SGFAIQTTKFISFLSSLKMRRSDLLQNRNFLREENIVWHGSDGSPPRWDGPSYRFLAMTL 446
            +GF IQTT+FISFL+SL+MRRSDLLQ R FL+EENI WHGSD SPP+WD PS RFLAMTL
Sbjct: 716  TGFGIQTTQFISFLTSLRMRRSDLLQKRTFLKEENIQWHGSDQSPPKWDDPSSRFLAMTL 775

Query: 445  KADSEESQSTSGSPEMIGDLFVAFNSAGHSENVALPPLTVDAAWFRLVDTSLPFPGFFTT 266
            KAD+E+SQ++    +  GDLFVAFNSAG SE+V LPP   D  W RLVDT+LPFP FF  
Sbjct: 776  KADAEDSQTSVS--DNGGDLFVAFNSAGESESVILPPPPTDMVWHRLVDTALPFPEFFAE 833

Query: 265  DGVPLKDGSVAYEMKSHSCVLFEARSL 185
             G P++DG VAYEMKSHSC+LFEA+ L
Sbjct: 834  KGTPVEDGLVAYEMKSHSCLLFEAQKL 860


>ref|XP_009761454.1| PREDICTED: isoamylase 2, chloroplastic [Nicotiana sylvestris]
            gi|698529252|ref|XP_009761455.1| PREDICTED: isoamylase 2,
            chloroplastic [Nicotiana sylvestris]
          Length = 863

 Score = 1004 bits (2597), Expect = 0.0
 Identities = 499/747 (66%), Positives = 583/747 (78%), Gaps = 6/747 (0%)
 Frame = -2

Query: 2407 LSSLHSGRDRDLVMSWGLFRSDSESFMPMDFQNSNADSKYSTIETPFVKDSSGRLVVELD 2228
            L S HS    +LVM WGLFRSD+  FMP+D     AD + ST+ETPFV+  S ++ VELD
Sbjct: 117  LPSEHSDAHAELVMLWGLFRSDASCFMPLDLNRRGADGQSSTLETPFVQGPSDKVTVELD 176

Query: 2227 FDESLAPFYVSILLKS-LGSNLESSEIRSHRKTNFVVPVGFRLGRPAPLGLSVLANGSMN 2051
            F+ SLAPFY+S  +KS L S+++ SEIRSHR TNFVVPVG   G P+PLGLS   +G+MN
Sbjct: 177  FEASLAPFYISFYMKSQLVSDMKRSEIRSHRNTNFVVPVGLTSGHPSPLGLSCQPDGTMN 236

Query: 2050 FAFFSRHAESVVLCLYADTSANKPALEIDLDPYVNRSGDIWHALLDDSLPFVSYGYRCKS 1871
            FA FSR A+SVVLCLY D S   P+LEIDLDPYVNRSGDIWHA LD SL F +YGYRCK 
Sbjct: 237  FALFSRSAKSVVLCLYDDISVEMPSLEIDLDPYVNRSGDIWHAALDCSLSFKTYGYRCKV 296

Query: 1870 NADKKRQYVLLDPYAKVIEDF-----GSGLPRKSVGKLWKEPGFDWSGDICPSLPMEKLI 1706
                K + VLLDPY+KVI        GS L  K +G+L KEPG+DWS D+ PSLPMEKLI
Sbjct: 297  ATSGKEELVLLDPYSKVIRSVIPRQGGSKLHPKYLGELCKEPGYDWSDDVPPSLPMEKLI 356

Query: 1705 IYRLNVTRFTKDKSSKLPGDVVGTFAGISEKLQHFKDLGVNAILLEPIFPFDEQKGPYFP 1526
            +YRLNVT FTKD SSKLP D+ GTF+GI+EK  HFK LGVNAILLEPIFPF+EQKGPYFP
Sbjct: 357  VYRLNVTEFTKDGSSKLPDDLAGTFSGITEKWSHFKHLGVNAILLEPIFPFEEQKGPYFP 416

Query: 1525 WHFFSPGSQYGPSGDSEFVVKSMKEMVKKLHANRIEVLLEVVFTHTAEVAALRQIDNSSY 1346
             HFFSPG+ +G S D   V+KSMK+MVKKLHAN IE+ LEV+FTHTAE A L  IDNS Y
Sbjct: 417  SHFFSPGNMHGRSSDPLSVIKSMKDMVKKLHANGIEIFLEVIFTHTAEDAPLMHIDNSCY 476

Query: 1345 YHVEGGEDLESRNALNCNYPIVQQLILDSLRHWVIEYHIDGFCFINASSLMKGFHGEFLS 1166
            Y ++GG D+  + ALNCNYPIVQQ+ILD LR+WVIE+HIDGF F+NASSL+KGFHGE LS
Sbjct: 477  YSIKGG-DMNIQFALNCNYPIVQQMILDCLRYWVIEFHIDGFVFVNASSLLKGFHGEILS 535

Query: 1165 RPPLVEAIAFDPLLSKVKIIADSWDPHNIEKKDISFPHWKRWAEINSKFCNDVRNFLRGQ 986
            RP LVEAIAFDP+LSKVKIIAD WD    + K+I FPHW++WAEIN++FC+D+R+FLRG+
Sbjct: 536  RPQLVEAIAFDPILSKVKIIADYWDALANDSKEILFPHWRKWAEINTRFCDDIRDFLRGK 595

Query: 985  GSLGNLATRLCGSGDLFLDGRGPAFSFNFISRNAGLPLVDXXXXXXXXXXXXXSWNCGEE 806
            G L +LATRLCGSGD+F  GRGPAFSFN+I+RN GL LVD             SWNCGEE
Sbjct: 596  GLLSSLATRLCGSGDIFSGGRGPAFSFNYIARNFGLTLVDLVSFSSSEVASELSWNCGEE 655

Query: 805  GPTNKTAVLERRLKQIRNYLLILFISLGVPILNMGDECGQSSGGSPAYADQKPLDWNALK 626
            GPT    VLE RLKQ+RN+L ILFISLGVP+LNMGDECGQSSGGSP +  +K  DW+ LK
Sbjct: 656  GPTTNNIVLETRLKQVRNFLFILFISLGVPVLNMGDECGQSSGGSPVHGARKSFDWSTLK 715

Query: 625  SGFAIQTTKFISFLSSLKMRRSDLLQNRNFLREENIVWHGSDGSPPRWDGPSYRFLAMTL 446
            +GF IQTT+FISFL+SL+MRRSDLLQ R FL+EENI WHGSD SPP+WD PS RFLAM+L
Sbjct: 716  TGFGIQTTQFISFLTSLRMRRSDLLQKRTFLKEENIQWHGSDQSPPKWDDPSSRFLAMSL 775

Query: 445  KADSEESQSTSGSPEMIGDLFVAFNSAGHSENVALPPLTVDAAWFRLVDTSLPFPGFFTT 266
            KAD+E SQ++    +  GDLFVAFNSAG SE+V LPP   D  W RLVDT+LPFPGFF  
Sbjct: 776  KADAEGSQTSVS--DNGGDLFVAFNSAGESESVILPPPPTDVVWHRLVDTALPFPGFFAE 833

Query: 265  DGVPLKDGSVAYEMKSHSCVLFEARSL 185
             G P++DG VAYEMK HSC+LFEA+ L
Sbjct: 834  KGTPVEDGLVAYEMKPHSCLLFEAQKL 860


>ref|XP_007201750.1| hypothetical protein PRUPE_ppa001199mg [Prunus persica]
            gi|462397150|gb|EMJ02949.1| hypothetical protein
            PRUPE_ppa001199mg [Prunus persica]
          Length = 883

 Score =  996 bits (2575), Expect = 0.0
 Identities = 495/756 (65%), Positives = 590/756 (78%), Gaps = 16/756 (2%)
 Frame = -2

Query: 2404 SSLHSGRDRDLVMSWGLFRSDSESFMPMDFQNSNADSKYSTIETPFVKDSSGRLVVELDF 2225
            SS  S  DR LV+ WG++R+DS  F+P+DF++S  +   +T+ETP ++ SSGR  +EL+F
Sbjct: 130  SSHLSSNDRRLVLRWGMYRADSSCFVPLDFKSSTPNDTTTTLETPLIQTSSGRFTLELEF 189

Query: 2224 DESLAPFYVSILLKS-LGSNLESSEIRSHRKTNFVVPVGFRLGRPAPLGLSVLANGSMNF 2048
            +    PFY S +L S   +N+   EIRSHRKTNF VPVGF  G P PLGL+   +GSMNF
Sbjct: 190  EAKQIPFYFSFILTSPADANVSDMEIRSHRKTNFCVPVGFGRGYPGPLGLTFSNDGSMNF 249

Query: 2047 AFFSRHAESVVLCLYADTSANKPALEIDLDPYVNRSGDIWHALLDDSLPFVSYGYRCKSN 1868
            A FSR+AESV LCLY +T+A KPALE+DLDPYVNRSGDIWH     +  FVSYGY+ K N
Sbjct: 250  AIFSRNAESVALCLYDNTTAEKPALELDLDPYVNRSGDIWHVSFGSAWSFVSYGYKFKGN 309

Query: 1867 A------DKKRQYVLLDPYAKVI-----EDFGSGLPRKSVGKLWKEPGFDWSGDICPSLP 1721
                   +    +VLLDPYAKVI      + G+GL  K +G+L +EP FDW+GD+ P L 
Sbjct: 310  LLLTNKNNFDEGHVLLDPYAKVIAKSIPNNHGTGL--KYLGRLCEEPAFDWAGDVRPDLS 367

Query: 1720 MEKLIIYRLNVTRFTKDKSSKLPGDVVGTFAGISEKLQHFKDLGVNAILLEPIFPFDEQK 1541
            MEKL++YRLNVTRFT+ KSS+LP ++ G+F+G++EKL+HFKDLGVNA+LLEPIFPFDEQK
Sbjct: 368  MEKLVVYRLNVTRFTEHKSSQLPTNIGGSFSGLTEKLEHFKDLGVNAVLLEPIFPFDEQK 427

Query: 1540 GPYFPWHFFSPGSQYGPSGDSEFVVKSMKEMVKKLHANRIEVLLEVVFTHTAEVAALRQI 1361
            GPYFP HFFSP   +GPS      V SMKEMV+K HAN IEVLLEVVFTHTAE  AL+ I
Sbjct: 428  GPYFPHHFFSPMDCFGPSRGPVSAVNSMKEMVRKFHANGIEVLLEVVFTHTAEGEALQGI 487

Query: 1360 DNSSYYHVEGGEDLESRNALNCNYPIVQQLILDSLRHWVIEYHIDGFCFINASSLMKGFH 1181
            D SSYYHV   EDLE+RNALNCNYPIVQQL+LDSLR+WV E+H+DGF FINASSL++GF+
Sbjct: 488  DISSYYHVNEVEDLEARNALNCNYPIVQQLVLDSLRYWVTEFHVDGFFFINASSLLRGFN 547

Query: 1180 GEFLSRPPLVEAIAFDPLLSKVKIIADSWDPHNIEKKDISFPHWKRWAEINSKFCNDVRN 1001
            GE+LSRPPLVEAIAFDPLLSK KIIAD WDPH +  K+  FPHW+RWAE+N+KFCNDVRN
Sbjct: 548  GEYLSRPPLVEAIAFDPLLSKTKIIADCWDPHGMAPKETHFPHWRRWAEVNTKFCNDVRN 607

Query: 1000 FLRGQGSLGNLATRLCGSGDLFLDGRGPAFSFNFISRNAGLPLVDXXXXXXXXXXXXXSW 821
            FLRG+G L +LATRLCG+GD+F DGRGPAF+FNFISRN+GLPLVD             SW
Sbjct: 608  FLRGEGLLSDLATRLCGNGDIFSDGRGPAFAFNFISRNSGLPLVDLVSFSGVELASELSW 667

Query: 820  NCGEEGPTNKTAVLERRLKQIRNYLLILFISLGVPILNMGDECGQSSGGSPAYADQKPLD 641
            NCGEEGPTNKTAVLERRLKQIRN+L ILF+SLGVP+LNMGDECGQS+GGSPAY+D+K  D
Sbjct: 668  NCGEEGPTNKTAVLERRLKQIRNFLFILFVSLGVPVLNMGDECGQSTGGSPAYSDRKAFD 727

Query: 640  WNALKSGFAIQTTKFISFLSSLKMRRSDLLQNRNFLREENIVWHGSDGSPPRWDGPSYRF 461
            WNAL++GFA QTT+FI+FLSS + RRSDLLQ RNFL+EENI W+ SD +PPRW+ PS +F
Sbjct: 728  WNALETGFATQTTQFIAFLSSFRKRRSDLLQKRNFLKEENIGWYESDQTPPRWEDPSRKF 787

Query: 460  LAMTLKADSEE-SQSTSGSPEMIGDLFVAFNSAGHSENVALPPLTVDAAWFRLVDTSLPF 284
            LAM LKAD +E +Q    S    GDLFVAF++A HSE V LPP      W RLVDT+LPF
Sbjct: 788  LAMRLKADEDEVNQPGDESSHSWGDLFVAFSAADHSETVVLPPPLEGMGWRRLVDTALPF 847

Query: 283  PGFFTTDGVPLKD---GSVAYEMKSHSCVLFEARSL 185
            PGFF+TDG P+ +   G  AYEMKSHSC LFEARSL
Sbjct: 848  PGFFSTDGEPVVEQIVGLFAYEMKSHSCALFEARSL 883


>ref|XP_008234909.1| PREDICTED: isoamylase 2, chloroplastic [Prunus mume]
          Length = 883

 Score =  996 bits (2574), Expect = 0.0
 Identities = 495/752 (65%), Positives = 587/752 (78%), Gaps = 16/752 (2%)
 Frame = -2

Query: 2392 SGRDRDLVMSWGLFRSDSESFMPMDFQNSNADSKYSTIETPFVKDSSGRLVVELDFDESL 2213
            S  DR LV+ WG++R+DS  FMP+DF++S  +   +T+ETPF++ SSGR  +EL+F+   
Sbjct: 134  SSSDRRLVLHWGMYRADSSCFMPLDFKSSTPNDTTTTLETPFIQTSSGRFTLELEFEAKQ 193

Query: 2212 APFYVSILLKS-LGSNLESSEIRSHRKTNFVVPVGFRLGRPAPLGLSVLANGSMNFAFFS 2036
             PFY S +L S   +N+   EIRSHRKTNF VPVGF  G P PLGL+   +GSMNFA FS
Sbjct: 194  IPFYFSFILTSPADANVSDMEIRSHRKTNFCVPVGFGRGYPGPLGLTFSNDGSMNFAIFS 253

Query: 2035 RHAESVVLCLYADTSANKPALEIDLDPYVNRSGDIWHALLDDSLPFVSYGYRCKSNA--- 1865
            R+AESVVLCLY +T+A KPALE+DLDPYVNRSGDIWH     +  FVSYGY+ K N    
Sbjct: 254  RNAESVVLCLYDNTTAEKPALELDLDPYVNRSGDIWHVSFGSAWTFVSYGYKFKRNLLLT 313

Query: 1864 ---DKKRQYVLLDPYAKVI-----EDFGSGLPRKSVGKLWKEPGFDWSGDICPSLPMEKL 1709
               +    +VLLDPYAKVI      + G+GL  K +G+L +EP FDW+GD+ P L MEKL
Sbjct: 314  NKNNFDEGHVLLDPYAKVIAKSIPNNHGTGL--KYLGRLCEEPAFDWAGDVRPDLSMEKL 371

Query: 1708 IIYRLNVTRFTKDKSSKLPGDVVGTFAGISEKLQHFKDLGVNAILLEPIFPFDEQKGPYF 1529
            ++YRLNVTRFT+ KSS+LP ++ G+F+G++EKL+HFKDLGVNA+LLEPIFPFDEQKGPYF
Sbjct: 372  VVYRLNVTRFTEHKSSQLPTNIGGSFSGLTEKLEHFKDLGVNAVLLEPIFPFDEQKGPYF 431

Query: 1528 PWHFFSPGSQYGPSGDSEFVVKSMKEMVKKLHANRIEVLLEVVFTHTAEVAALRQIDNSS 1349
            P HFFSP    GPS      V SMKEMV+K HAN IEVLLEVVFTHTAE  AL+ ID SS
Sbjct: 432  PRHFFSPMECIGPSRGPVSAVNSMKEMVRKFHANGIEVLLEVVFTHTAEGEALQGIDISS 491

Query: 1348 YYHVEGGEDLESRNALNCNYPIVQQLILDSLRHWVIEYHIDGFCFINASSLMKGFHGEFL 1169
            YYHV   EDLE+RNALNCNYPIVQQL+LDSLR+WV E+HIDGFCFINASSL++GF+GE+L
Sbjct: 492  YYHVNEVEDLEARNALNCNYPIVQQLVLDSLRYWVTEFHIDGFCFINASSLLRGFNGEYL 551

Query: 1168 SRPPLVEAIAFDPLLSKVKIIADSWDPHNIEKKDISFPHWKRWAEINSKFCNDVRNFLRG 989
            SRPPLVEAIAFDPLLSK KIIAD WDPH +  K+  FPHWKRWAE+N+KFCNDVRN+LRG
Sbjct: 552  SRPPLVEAIAFDPLLSKTKIIADCWDPHGMAPKETRFPHWKRWAEVNTKFCNDVRNYLRG 611

Query: 988  QGSLGNLATRLCGSGDLFLDGRGPAFSFNFISRNAGLPLVDXXXXXXXXXXXXXSWNCGE 809
            +G L +LATRLCG+GD+F DGRGPAF+FNFISRN+GLPLVD             SWNCGE
Sbjct: 612  KGLLSDLATRLCGNGDIFSDGRGPAFAFNFISRNSGLPLVDLVSFSGVELASELSWNCGE 671

Query: 808  EGPTNKTAVLERRLKQIRNYLLILFISLGVPILNMGDECGQSSGGSPAYADQKPLDWNAL 629
            EGPTNKTAVLERRLKQIRN+L ILF+SLGVP+LN GDECGQS+GGSPAY+D+K  DWNAL
Sbjct: 672  EGPTNKTAVLERRLKQIRNFLFILFVSLGVPVLNTGDECGQSTGGSPAYSDRKAFDWNAL 731

Query: 628  KSGFAIQTTKFISFLSSLKMRRSDLLQNRNFLREENIVWHGSDGSPPRWDGPSYRFLAMT 449
            ++GFA QTT+FI+FLSS + RRSDLLQ RNFL+EENI W+ SD +PPRW+ PS +FLAM 
Sbjct: 732  ETGFATQTTQFIAFLSSFRKRRSDLLQKRNFLKEENIDWYESDQTPPRWEDPSRKFLAMR 791

Query: 448  LKADSEE-SQSTSGSPEMIGDLFVAFNSAGHSENVALPPLTVDAAWFRLVDTSLPFPGFF 272
            LKAD +E +Q    S    GDLF AF++A HSE + LPP      W RLVDT+LPFPGFF
Sbjct: 792  LKADEDEVNQPGDESSHSWGDLFAAFSAADHSETLVLPPPREGMGWRRLVDTALPFPGFF 851

Query: 271  TTDGVPL---KDGSVAYEMKSHSCVLFEARSL 185
            +TDG P+     G  AYEMKSHSC LFEARSL
Sbjct: 852  STDGEPVVRQMVGLFAYEMKSHSCALFEARSL 883


>ref|XP_008386532.1| PREDICTED: isoamylase 2, chloroplastic-like [Malus domestica]
          Length = 870

 Score =  985 bits (2546), Expect = 0.0
 Identities = 492/751 (65%), Positives = 586/751 (78%), Gaps = 10/751 (1%)
 Frame = -2

Query: 2407 LSSLH-SGRDRDLVMSWGLFRSDSESFMPMDFQNSNADSKYSTIETPFVKDSSGRLVVEL 2231
            +SSLH S  DR LV+SWG++RSDS SFMP +F++S    + +T+ETPF +  SGR  +EL
Sbjct: 129  VSSLHLSSNDRLLVLSWGIYRSDSSSFMPSNFRSSTPADRTTTLETPFTETCSGRFTLEL 188

Query: 2230 DFDESLAPFYVSILLKSLGSNLESSE--IRSHRKTNFVVPVGFRLGRPAPLGLSVLANGS 2057
            +F+    PFY+S +LKS  ++ +SS+  IRSHRKTNF  PVGF  G PAPLGLS   +GS
Sbjct: 189  EFEAKQIPFYLSFILKS-PADADSSDLDIRSHRKTNFCFPVGFGRGYPAPLGLSFSNDGS 247

Query: 2056 MNFAFFSRHAESVVLCLYADTSANKPALEIDLDPYVNRSGDIWHALLDDSLPFVSYGYRC 1877
            MNFA FSR+AESVVLCLY +T+A KP LE+DLDPYVNRSGDIWHA  +    FVSYGYR 
Sbjct: 248  MNFAIFSRNAESVVLCLYGETTAEKPVLELDLDPYVNRSGDIWHASFESGWDFVSYGYRF 307

Query: 1876 KSNADKKRQYVLLDPYAKVIE---DFGSGLPRKSVGKLWKEPGFDWSGDICPSLPMEKLI 1706
                      VLLDPYAK+I      G+GL  K +G+L +EP F+W+GD+ P L MEKL+
Sbjct: 308  DEGN------VLLDPYAKIISRSVPHGTGL--KYLGRLCEEPAFNWAGDVRPDLAMEKLV 359

Query: 1705 IYRLNVTRFTKDKSSKLPGDVVGTFAGISEKLQHFKDLGVNAILLEPIFPFDEQKGPYFP 1526
            +YRLNVTRFT+ KSS LP ++ GTF+G++EKL+H K LGVNA+LLEPIFPFDEQKGPYFP
Sbjct: 360  VYRLNVTRFTEHKSSNLPTNIAGTFSGLTEKLEHLKHLGVNAVLLEPIFPFDEQKGPYFP 419

Query: 1525 WHFFSPGSQYGPSGDSEFVVKSMKEMVKKLHANRIEVLLEVVFTHTAEVAALRQIDNSSY 1346
             HFFSP + +GPS      V SMK+MVKK HA+ IEVLLEVVFTHTAE  AL+ ID SSY
Sbjct: 420  IHFFSPMNWFGPSRGPVSAVNSMKDMVKKFHADGIEVLLEVVFTHTAEGEALQGIDISSY 479

Query: 1345 YHVEGGEDLESRNALNCNYPIVQQLILDSLRHWVIEYHIDGFCFINASSLMKGFHGEFLS 1166
            YH+ G  DL++RNALNCNYP+VQQ++LDSLR+WV E+H+DGFCFINASSLM+G  GE+LS
Sbjct: 480  YHINGVADLKARNALNCNYPVVQQMVLDSLRYWVTEFHVDGFCFINASSLMRGSKGEYLS 539

Query: 1165 RPPLVEAIAFDPLLSKVKIIADSWDPHNIEKKDISFPHWKRWAEINSKFCNDVRNFLRGQ 986
            RPPLVEAIAFDPLLSK KIIAD WDPH    K+  FPHWKRWAE+NSKF  DVRNFLRG+
Sbjct: 540  RPPLVEAIAFDPLLSKTKIIADRWDPHGSVPKETRFPHWKRWAEVNSKFSKDVRNFLRGE 599

Query: 985  GSLGNLATRLCGSGDLFLDGRGPAFSFNFISRNAGLPLVDXXXXXXXXXXXXXSWNCGEE 806
            G L +LATRLCG+GD+F DGRGPAF+FNFISRN+GLPLVD             SWNCGEE
Sbjct: 600  GLLSDLATRLCGNGDIFSDGRGPAFAFNFISRNSGLPLVDLVSFSGVKLASELSWNCGEE 659

Query: 805  GPTNKTAVLERRLKQIRNYLLILFISLGVPILNMGDECGQSSGGSPAYADQKPLDWNALK 626
            GPT+KTAVLERRLKQIRN+L ILF+SLGVP+LNMGDECGQS+GGSPAY+D+K  DWNAL 
Sbjct: 660  GPTDKTAVLERRLKQIRNFLFILFVSLGVPVLNMGDECGQSTGGSPAYSDRKAFDWNALG 719

Query: 625  SGFAIQTTKFISFLSSLKMRRSDLLQNRNFLREENIVWHGSDGSPPRWDGPSYRFLAMTL 446
            +GFA QTT+FI+FLSS ++RRSDLLQ RNFL+EENI W+GSD S P+W+ PS +FLAM L
Sbjct: 720  TGFATQTTQFIAFLSSFRIRRSDLLQERNFLKEENIDWYGSDQSSPKWEDPSCKFLAMKL 779

Query: 445  KADSEESQSTSG-SPEMIGDLFVAFNSAGHSENVALPPLTVDAAWFRLVDTSLPFPGFFT 269
            K D EE+      SP + GDLFVAF++A  SE V LPP      WFRLVDT+LPFPGFF+
Sbjct: 780  KPDEEEATEPGDVSPPIWGDLFVAFSAAARSETVILPPPPEGMGWFRLVDTALPFPGFFS 839

Query: 268  TDGVPLKD---GSVAYEMKSHSCVLFEARSL 185
            TDG P+ +   G  AY+MKSHSC LFEAR L
Sbjct: 840  TDGEPVPEQMAGLFAYQMKSHSCALFEARCL 870


>ref|XP_009347699.1| PREDICTED: isoamylase 2, chloroplastic-like [Pyrus x bretschneideri]
          Length = 869

 Score =  984 bits (2543), Expect = 0.0
 Identities = 491/748 (65%), Positives = 587/748 (78%), Gaps = 7/748 (0%)
 Frame = -2

Query: 2407 LSSLHSGR-DRDLVMSWGLFRSDSESFMPMDFQNSNADSKYSTIETPFVKDSSGRLVVEL 2231
            +S LH  R DR LV+SWG++R+DS SF+P +F++S    + + +ETPF   SSG+  +EL
Sbjct: 129  VSLLHPSRSDRRLVLSWGMYRADSSSFVPSNFRSSTPADRTTALETPFTGTSSGKFTLEL 188

Query: 2230 DFDESLAPFYVSILLKS-LGSNLESSEIRSHRKTNFVVPVGFRLGRPAPLGLSVLANGSM 2054
            +F+    PFY+S +LKS   ++L   EIRSHRKTNF  PVGF  G PAPLGLS   +GSM
Sbjct: 189  EFEAKQIPFYLSFVLKSPADADLSDLEIRSHRKTNFCFPVGFGRGCPAPLGLSFSNDGSM 248

Query: 2053 NFAFFSRHAESVVLCLYADTSANKPALEIDLDPYVNRSGDIWHALLDDSLPFVSYGYRCK 1874
            NFA FSR+AESVVLCLY +T+A KPALE+DLDPYVNRSGDIWHA  + +  FVSYGYR  
Sbjct: 249  NFAIFSRNAESVVLCLYDETTAEKPALELDLDPYVNRSGDIWHASFEKAWDFVSYGYRFD 308

Query: 1873 SNADKKRQYVLLDPYAKVI-EDFGSGLPRKSVGKLWKEPGFDWSGDICPSLPMEKLIIYR 1697
                     VLLDPYAK+I +    G   K +G+L +EP FDW+GD+ P+L +EKL++YR
Sbjct: 309  EGN------VLLDPYAKIIAKSVPHGTGSKYLGRLREEPAFDWAGDVLPNLTLEKLVVYR 362

Query: 1696 LNVTRFTKDKSSKLPGDVVGTFAGISEKLQHFKDLGVNAILLEPIFPFDEQKGPYFPWHF 1517
            LNV RFT+ KSSKLP ++ GTF+G+++KL+HFK LGVNA+LLEPIFPFDE KGPYFP HF
Sbjct: 363  LNVKRFTEHKSSKLPTNIAGTFSGLTQKLEHFKALGVNAVLLEPIFPFDEHKGPYFPVHF 422

Query: 1516 FSPGSQYGPSGDSEFVVKSMKEMVKKLHANRIEVLLEVVFTHTAEVAALRQIDNSSYYHV 1337
            FSP + +GPSG     V SMKE VKK HA+ +EVLLEVVFTHTAE  A++ ID SSYYHV
Sbjct: 423  FSPMNCFGPSGPVS-AVNSMKETVKKFHADGVEVLLEVVFTHTAEGEAMQGIDISSYYHV 481

Query: 1336 EGGEDLESRNALNCNYPIVQQLILDSLRHWVIEYHIDGFCFINASSLMKGFHGEFLSRPP 1157
             G EDLE+RNALNCNYP+VQQ++LDSLR+WV E+H+DGFCFINASSL++G +GE LSRPP
Sbjct: 482  NGVEDLEARNALNCNYPVVQQMVLDSLRYWVTEFHVDGFCFINASSLLRGSNGECLSRPP 541

Query: 1156 LVEAIAFDPLLSKVKIIADSWDPHNIEKKDISFPHWKRWAEINSKFCNDVRNFLRGQGSL 977
            LVEAIAFDPLLSK KIIAD WDP  +  K+  FPHWKRWAE+N+KF NDVRNFLRG+G L
Sbjct: 542  LVEAIAFDPLLSKAKIIADCWDPRGLVPKETRFPHWKRWAEVNTKFSNDVRNFLRGEGLL 601

Query: 976  GNLATRLCGSGDLFLDGRGPAFSFNFISRNAGLPLVDXXXXXXXXXXXXXSWNCGEEGPT 797
            G+LATRLCG+GD+F DGRGPAFSFNFISRN+GLPLVD             SWNC EEGPT
Sbjct: 602  GDLATRLCGNGDIFSDGRGPAFSFNFISRNSGLPLVDLVSFSGVELAAELSWNCEEEGPT 661

Query: 796  NKTAVLERRLKQIRNYLLILFISLGVPILNMGDECGQSSGGSPAYADQKPLDWNALKSGF 617
            NKTAVLERRLKQIRN+L ILF+SLGVP+LNMGDECGQS+GGS AY+D+K  DWNAL +GF
Sbjct: 662  NKTAVLERRLKQIRNFLFILFVSLGVPVLNMGDECGQSTGGSTAYSDRKAFDWNALGAGF 721

Query: 616  AIQTTKFISFLSSLKMRRSDLLQNRNFLREENIVWHGSDGSPPRWDGPSYRFLAMTLKAD 437
            A QTT+FI+FLSS ++RRSDLLQ RNFL+EENI W+GSD S P+W+ PS +FLAM LKAD
Sbjct: 722  ATQTTQFIAFLSSFRIRRSDLLQKRNFLKEENIDWYGSDQSSPKWEDPSCKFLAMKLKAD 781

Query: 436  SEE-SQSTSGSPEMIGDLFVAFNSAGHSENVALPPLTVDAAWFRLVDTSLPFPGFFTTDG 260
             EE ++S   S    GDLFVAF++A  SE V LPPL     WFRLVDT+LPFPGFF+TDG
Sbjct: 782  EEEANESGDDSSPSWGDLFVAFSAADLSETVILPPLPEGMGWFRLVDTALPFPGFFSTDG 841

Query: 259  VPLKD---GSVAYEMKSHSCVLFEARSL 185
             P+ +   G  AYEMKSHSC LFEARSL
Sbjct: 842  EPVAEQPAGLFAYEMKSHSCALFEARSL 869


>ref|XP_002271798.1| PREDICTED: isoamylase 2, chloroplastic [Vitis vinifera]
          Length = 882

 Score =  981 bits (2536), Expect = 0.0
 Identities = 487/747 (65%), Positives = 584/747 (78%), Gaps = 14/747 (1%)
 Frame = -2

Query: 2383 DRDLVMSWGLFRSDSESFMPMDFQNSNADSKYSTIETPFVKDSSGRLVVELDFDESLAPF 2204
            D  L++SWG+FRS+S  FMP+DFQN   +   +T E PF++ SSG   ++LDF+ + APF
Sbjct: 136  DNKLILSWGVFRSNSSCFMPVDFQNLVPEVGSNTTEIPFMERSSGSFALKLDFEANHAPF 195

Query: 2203 YVSILLKS-LGSNLESSEIRSHRKTNFVVPVGFRLGRPAPLGLSVLANGSMNFAFFSRHA 2027
            Y+S LLKS L ++L SS+IRSHRKTNF +PVGF  G PAPLGLS  ++GS NFAFFSR+A
Sbjct: 196  YLSFLLKSTLDTDLSSSDIRSHRKTNFCIPVGFGRGYPAPLGLSFSSDGSPNFAFFSRNA 255

Query: 2026 ESVVLCLYADTSANKPALEIDLDPYVNRSGDIWHALLDDSLPFVSYGYRCK------SNA 1865
              VVLCLY  TS +KPALEIDLDPYVNR+GDIWHA ++    FVSYGYRCK      S  
Sbjct: 256  GGVVLCLYDGTS-DKPALEIDLDPYVNRTGDIWHASMESVGSFVSYGYRCKEANLQDSGE 314

Query: 1864 DKKRQYVLLDPYAKVIEDFGS---GL-PRKSVGKLWKEPGFDWSGDICPSLPMEKLIIYR 1697
                ++V LDPYAK+I +  S   GL P+  +G+L KEP F+W+ D+ P +PMEKL++YR
Sbjct: 315  TLHTEHVHLDPYAKLIRNSFSDDHGLKPQPRLGELQKEPAFNWNDDVHPYIPMEKLVVYR 374

Query: 1696 LNVTRFTKDKSSKLPGDVVGTFAGISEKLQHFKDLGVNAILLEPIFPFDEQKGPYFPWHF 1517
            LNV  FTKD+SS++  D+ GTF+G+ EKL HFKDLGVNA+LLEPIF FDEQKGPYFP+HF
Sbjct: 375  LNVMHFTKDESSQVASDLAGTFSGLMEKLHHFKDLGVNAVLLEPIFSFDEQKGPYFPFHF 434

Query: 1516 FSPGSQYGPSGDSEFVVKSMKEMVKKLHANRIEVLLEVVFTHTAEVAALRQIDNSSYYHV 1337
            FSP + YGPS      + S+KEMVK+LHAN IEVLLEVVFTHTAE  AL+ ID+S YY+V
Sbjct: 435  FSPMNVYGPSSGPVSTINSVKEMVKRLHANGIEVLLEVVFTHTAESGALQGIDDSCYYYV 494

Query: 1336 EGGEDLESRNALNCNYPIVQQLILDSLRHWVIEYHIDGFCFINASSLMKGFHGEFLSRPP 1157
             G  DL  RNALNCNY IVQQ+I+DSLR+WV E+H+DGFCFINASSL++GFHGE+LSRPP
Sbjct: 495  NGDADLGIRNALNCNYSIVQQMIVDSLRYWVTEFHVDGFCFINASSLLRGFHGEYLSRPP 554

Query: 1156 LVEAIAFDPLLSKVKIIADSWDPHNIEKKDISFPHWKRWAEINSKFCNDVRNFLRGQGSL 977
            LVE IAFDPLLSK KIIAD WDP N+  K+I FPHWKRWAE+N++FCNDVRNFLRG+G L
Sbjct: 555  LVETIAFDPLLSKTKIIADCWDPRNMLPKEIRFPHWKRWAEVNTRFCNDVRNFLRGEG-L 613

Query: 976  GNLATRLCGSGDLFLDGRGPAFSFNFISRNAGLPLVDXXXXXXXXXXXXXSWNCGEEGPT 797
             + ATRLCGSGD+F+DGRGPAFSFNF ++N GLPLVD             SWNCG+EGPT
Sbjct: 614  SDFATRLCGSGDIFMDGRGPAFSFNFTTKNFGLPLVDLVSFSSSELASELSWNCGDEGPT 673

Query: 796  NKTAVLERRLKQIRNYLLILFISLGVPILNMGDECGQSSGGSPAYADQKPLDWNALKSGF 617
            NKT VLERRLKQIRN+L IL++SLGVPILNMGDECGQSSGGSPAY D+KP +WN++K+GF
Sbjct: 674  NKTTVLERRLKQIRNFLFILYVSLGVPILNMGDECGQSSGGSPAYGDRKPFNWNSVKTGF 733

Query: 616  AIQTTKFISFLSSLKMRRSDLLQNRNFLREENIVWHGSDGSPPRWDGPSYRFLAMTLKAD 437
             IQT +FISFLSSL+ RRSDLLQ R+FL+EE+I WHGSD SPPRWD PS +FLAMTLKA+
Sbjct: 734  GIQTIQFISFLSSLRSRRSDLLQRRSFLKEESIDWHGSDQSPPRWDDPSSKFLAMTLKAE 793

Query: 436  SEESQSTSGSPEMIGDLFVAFNSAGHSENVALPPLTVDAAWFRLVDTSLPFPGFFTTDGV 257
            + E Q  S S  + GDLF+AFN+A  S  V LPP      W RLVDT+LPFPGFFT DG 
Sbjct: 794  NMEGQLPSESSSIKGDLFIAFNTADRSVKVILPPPPTGMVWHRLVDTALPFPGFFTADGE 853

Query: 256  PL---KDGSVAYEMKSHSCVLFEARSL 185
             +   K G V Y+M+SHSC LFEA +L
Sbjct: 854  AILKKKSGLVTYKMESHSCALFEANTL 880


>ref|XP_009356744.1| PREDICTED: isoamylase 2, chloroplastic-like [Pyrus x bretschneideri]
          Length = 870

 Score =  976 bits (2524), Expect = 0.0
 Identities = 488/751 (64%), Positives = 586/751 (78%), Gaps = 10/751 (1%)
 Frame = -2

Query: 2407 LSSLH-SGRDRDLVMSWGLFRSDSESFMPMDFQNSNADSKYSTIETPFVKDSSGRLVVEL 2231
            +SSLH S  DR LV+SWG++RSD+ SFMP +F++S    + +T+ETPF + SSGR  +EL
Sbjct: 129  VSSLHLSSNDRLLVLSWGIYRSDASSFMPSNFRSSTPADRTTTLETPFTETSSGRFTLEL 188

Query: 2230 DFDESLAPFYVSILLKSLGSNLESS--EIRSHRKTNFVVPVGFRLGRPAPLGLSVLANGS 2057
            +F+    PFY+S +LKS  ++ +SS  EIRSHRKTNF  PVGF  G PAPLGLS   +GS
Sbjct: 189  EFEAKQIPFYLSFILKS-PADADSSDLEIRSHRKTNFCFPVGFSRGNPAPLGLSFSNDGS 247

Query: 2056 MNFAFFSRHAESVVLCLYADTSANKPALEIDLDPYVNRSGDIWHALLDDSLPFVSYGYRC 1877
            MNFA FSR+AESVVLCLY +T+A KP LE+DLDPYVNRSGDIWHA  + +  FVSYGYR 
Sbjct: 248  MNFAIFSRNAESVVLCLYGETTAEKPVLELDLDPYVNRSGDIWHASFESAWDFVSYGYRF 307

Query: 1876 KSNADKKRQYVLLDPYAKVIED---FGSGLPRKSVGKLWKEPGFDWSGDICPSLPMEKLI 1706
                      VLLDPYAK+I      G+GL  K +G+L +EP F+W+GD+ P L MEKL+
Sbjct: 308  DEGN------VLLDPYAKIIAKSVPHGTGL--KYLGRLCEEPAFNWAGDVRPDLAMEKLV 359

Query: 1705 IYRLNVTRFTKDKSSKLPGDVVGTFAGISEKLQHFKDLGVNAILLEPIFPFDEQKGPYFP 1526
            +YRLNV RFT+ KSSKLP ++ GTF+G++EKL+H KDLGVNA+LLEPIFPFDEQKGPYFP
Sbjct: 360  VYRLNVMRFTEHKSSKLPTNIAGTFSGLTEKLEHLKDLGVNAVLLEPIFPFDEQKGPYFP 419

Query: 1525 WHFFSPGSQYGPSGDSEFVVKSMKEMVKKLHANRIEVLLEVVFTHTAEVAALRQIDNSSY 1346
             HFFSP + +GPS      V SMK+MVKK HA+ +EVLLEVVFTHTAE  AL+ ID SSY
Sbjct: 420  IHFFSPMNCFGPSRGPVSAVNSMKDMVKKFHADGMEVLLEVVFTHTAEGEALQGIDISSY 479

Query: 1345 YHVEGGEDLESRNALNCNYPIVQQLILDSLRHWVIEYHIDGFCFINASSLMKGFHGEFLS 1166
            Y +    DL++RNALNCNY +VQQ++LDSLR+WV E+H+DGFCFINASSL++G +GE+LS
Sbjct: 480  YRINRVADLKARNALNCNYLVVQQMVLDSLRYWVTEFHVDGFCFINASSLLRGSNGEYLS 539

Query: 1165 RPPLVEAIAFDPLLSKVKIIADSWDPHNIEKKDISFPHWKRWAEINSKFCNDVRNFLRGQ 986
            RPPLVEAIAFDPLLSK KIIAD WDPH    K+   PHWKRWAE+NSKF  DVRNFLRG 
Sbjct: 540  RPPLVEAIAFDPLLSKTKIIADCWDPHGSVPKETRLPHWKRWAEVNSKFSKDVRNFLRGG 599

Query: 985  GSLGNLATRLCGSGDLFLDGRGPAFSFNFISRNAGLPLVDXXXXXXXXXXXXXSWNCGEE 806
            G L +LATRLCG+GD+F DGRGPAFSFNFISRN+GLPLVD             SWNCG+E
Sbjct: 600  GLLSDLATRLCGNGDIFSDGRGPAFSFNFISRNSGLPLVDLVSFSGVELASELSWNCGKE 659

Query: 805  GPTNKTAVLERRLKQIRNYLLILFISLGVPILNMGDECGQSSGGSPAYADQKPLDWNALK 626
            GPT+KTAVLERRLKQIRN+L ILF+SLGVP+LNMGDECGQS+GGSPAY+D+K  DWNAL 
Sbjct: 660  GPTDKTAVLERRLKQIRNFLFILFVSLGVPVLNMGDECGQSTGGSPAYSDRKAFDWNALG 719

Query: 625  SGFAIQTTKFISFLSSLKMRRSDLLQNRNFLREENIVWHGSDGSPPRWDGPSYRFLAMTL 446
            +GFA QTT+FI+FLSS +++RSDLL  RNFL+EENI W+GSD S P+W+ PS +FLAM L
Sbjct: 720  TGFATQTTQFIAFLSSFRIKRSDLLHRRNFLKEENIDWYGSDQSSPKWEDPSCKFLAMKL 779

Query: 445  KADSEESQSTSG-SPEMIGDLFVAFNSAGHSENVALPPLTVDAAWFRLVDTSLPFPGFFT 269
            K D EE+      SP + GDLFVAF++A HSE V LPP      WFRLVDT+LPFPGFF+
Sbjct: 780  KPDEEEANEPGDVSPPLWGDLFVAFSAADHSETVILPPPPEGMGWFRLVDTALPFPGFFS 839

Query: 268  TDGVPLKD---GSVAYEMKSHSCVLFEARSL 185
            TDG P+ +   G  AY+MKSHSC LFEARSL
Sbjct: 840  TDGEPVPEQIAGLFAYQMKSHSCALFEARSL 870


>ref|XP_007050677.1| Debranching enzyme 1 [Theobroma cacao] gi|508702938|gb|EOX94834.1|
            Debranching enzyme 1 [Theobroma cacao]
          Length = 867

 Score =  973 bits (2515), Expect = 0.0
 Identities = 486/751 (64%), Positives = 581/751 (77%), Gaps = 11/751 (1%)
 Frame = -2

Query: 2407 LSSLH-SGRDRDLVMSWGLFRSDSESFMPMDFQNSNADSKYSTIETPFVKDSSGRLVVEL 2231
            +SSL  SG +  LV+S G+ RSD              D K   IETPF+  SS  L +EL
Sbjct: 127  VSSLQLSGDESQLVLSGGIHRSDH-------------DIKNRIIETPFIAKSSSELALEL 173

Query: 2230 DFDESLAPFYVSILLKS-LGSNLESSEIRSHRKTNFVVPVGFRLGRPAPLGLSVLANGSM 2054
            +F+   APFY S LLK+  G+NL  SEIR+HRKTNF VPVGF  G PAPLGLS   +GSM
Sbjct: 174  EFEAKEAPFYFSFLLKAPSGANLSGSEIRTHRKTNFCVPVGFNQGYPAPLGLSFSTDGSM 233

Query: 2053 NFAFFSRHAESVVLCLYADTSANKPALEIDLDPYVNRSGDIWHALLDDSLPFVSYGYRCK 1874
            NFA +SR+AES+VLCLY DT++ KPALE+DLDP+VNR+GDIWHA ++ S  FV YGYRCK
Sbjct: 234  NFAVYSRNAESLVLCLYDDTASEKPALELDLDPFVNRTGDIWHASIEGSWTFVRYGYRCK 293

Query: 1873 SNADK-KRQYVLLDPYAKVI-----EDFGSGLPRKSVGKLWKEPGFDWSGDICPSLPMEK 1712
             + D    + VLLDPYA++I         SGL  K +G+L KEP FDWS D+CP+LPMEK
Sbjct: 294  GDTDAFNAERVLLDPYARIIGSSIPNHHESGLLLKHLGRLCKEPAFDWSSDVCPNLPMEK 353

Query: 1711 LIIYRLNVTRFTKDKSSKLPGDVVGTFAGISEKLQHFKDLGVNAILLEPIFPFDEQKGPY 1532
            L++YRLNV  FT+DKSSKLP DVVGTFAG++EK+QH KDLGVNA+LLEPIF FDEQKGPY
Sbjct: 354  LVVYRLNVLHFTEDKSSKLPADVVGTFAGVTEKVQHLKDLGVNAVLLEPIFTFDEQKGPY 413

Query: 1531 FPWHFFSPGSQYGPSGDSEFVVKSMKEMVKKLHANRIEVLLEVVFTHTAEVAALRQIDNS 1352
            FP HFFSP + YGPS  S   + S+KEMVK LHAN +EVLLEVVFTHTAE  AL+ +D+ 
Sbjct: 414  FPCHFFSPTNLYGPSNGSVSAINSIKEMVKNLHANGMEVLLEVVFTHTAEGGALQGLDDL 473

Query: 1351 SYYHVEGGEDLESRNALNCNYPIVQQLILDSLRHWVIEYHIDGFCFINASSLMKGFHGEF 1172
            SYY+    +DLE RNALNCNYP+VQ++ILDSLRHWV E+HIDGFCFINAS L++GF+GE 
Sbjct: 474  SYYYRNRVQDLERRNALNCNYPVVQRMILDSLRHWVTEFHIDGFCFINASCLLRGFYGEH 533

Query: 1171 LSRPPLVEAIAFDPLLSKVKIIADSWDPHNIEKKDISFPHWKRWAEINSKFCNDVRNFLR 992
            LSRPPL+EAIAFDPLLSK KIIAD W PH++  K+I FPHWK+WAE+N+KFC D+RNFLR
Sbjct: 534  LSRPPLIEAIAFDPLLSKTKIIADCWYPHDMMPKEIRFPHWKKWAEMNTKFCTDIRNFLR 593

Query: 991  GQGSLGNLATRLCGSGDLFLDGRGPAFSFNFISRNAGLPLVDXXXXXXXXXXXXXSWNCG 812
            G+G+L +LATRLCGSGD+F DGRGPAFSFN+I++N GLPLVD             SWNCG
Sbjct: 594  GEGALSSLATRLCGSGDIFSDGRGPAFSFNYIAKNFGLPLVDLVSFSKAEIASELSWNCG 653

Query: 811  EEGPTNKTAVLERRLKQIRNYLLILFISLGVPILNMGDECGQSSGGSPAYADQKPLDWNA 632
             EGPTNKTAVLERRLKQIRN+L ILFISLGVP+LNMGDECGQSSGGS +Y  +K LDWNA
Sbjct: 654  VEGPTNKTAVLERRLKQIRNFLFILFISLGVPVLNMGDECGQSSGGSLSYGSRKLLDWNA 713

Query: 631  LKSGFAIQTTKFISFLSSLKMRRSDLLQNRNFLREENIVWHGSDGSPPRWDGPSYRFLAM 452
            + +GF IQTT+FISFLSSL+MRRSDLLQ R+FL+EENI W+GSD SPP W+ PS +FLAM
Sbjct: 714  MTTGFGIQTTQFISFLSSLRMRRSDLLQKRSFLKEENIEWYGSDQSPPGWEDPSCKFLAM 773

Query: 451  TLKADSEESQSTSGSPEMIGDLFVAFNSAGHSENVALPPLTVDAAWFRLVDTSLPFPGFF 272
            TLKAD EE+Q +S +  + GDL +A N+A  +E + LPP     AW RLVDT+LP+PGFF
Sbjct: 774  TLKADKEENQLSSEASRLKGDLLIAINAADKAEIIILPPPPEGLAWRRLVDTALPYPGFF 833

Query: 271  TTDGVPLKD---GSVAYEMKSHSCVLFEARS 188
            + DG  + +   G  AYEMKS SC LFEAR+
Sbjct: 834  SADGKAVLEQMMGLFAYEMKSLSCTLFEARA 864


>ref|XP_010090367.1| Isoamylase 2 [Morus notabilis] gi|587849097|gb|EXB39337.1| Isoamylase
            2 [Morus notabilis]
          Length = 825

 Score =  964 bits (2492), Expect = 0.0
 Identities = 489/756 (64%), Positives = 580/756 (76%), Gaps = 16/756 (2%)
 Frame = -2

Query: 2407 LSSLHSGR-DRDLVMSWGLFRSDSESFMPMDFQNSNADSKYSTIETPFVKDSSGRLVVEL 2231
            +SSL   R D  L +SWG++RSDS SF+P  F+ S      + +ETPF K+S GR  VE 
Sbjct: 80   VSSLELPRSDGTLGLSWGIYRSDSSSFLPSHFETS------TPVETPFTKNSFGRYTVEF 133

Query: 2230 DFDESLAPFYVSILLKSLGSNLESS-EIRSHRKTNFVVPVGFRLGRPAPLGLSVLANGSM 2054
            +F+    P Y+S LLKS   N  S  +IRSHRKTNF VP+GF  G P PLGLS   +GS+
Sbjct: 134  EFEAKEIPCYLSFLLKSPRDNDSSGLDIRSHRKTNFCVPLGFSSGHPTPLGLSFSRDGSL 193

Query: 2053 NFAFFSRHAESVVLCLYADTSANKPALEIDLDPYVNRSGDIWHALLDDSLPFVSYGYRCK 1874
            NF+ FSR AESVVLCLY DT+A+ PALE+DLDPYVNR+GDIWHA  + S  FVSYGYR K
Sbjct: 194  NFSIFSRSAESVVLCLYDDTTADNPALELDLDPYVNRTGDIWHASFESSSTFVSYGYRLK 253

Query: 1873 SNADKKRQ----YVLLDPYAKVIEDFGS---GLPRKSVGKLWKEPGFDWSGDICPSLPME 1715
             +  K ++     ++LDPYAK+I    S   G+  K +G++ KEPGFDWSGDI P+LP+E
Sbjct: 254  GSRLKGKKDEDARIVLDPYAKIIGKSTSSDHGIGPKYLGRISKEPGFDWSGDIRPNLPIE 313

Query: 1714 KLIIYRLNVTRFTKDKSSKLPGDVVGTFAGISEKLQHFKDLGVNAILLEPIFPFDEQKGP 1535
            KL +YRLNV  FT+ KSS+L  D+ GTF+G+ EKL+HF DLGVNA+LLEPIFPFDEQKGP
Sbjct: 314  KLTVYRLNVMHFTEHKSSQLSPDIAGTFSGLIEKLEHFTDLGVNAVLLEPIFPFDEQKGP 373

Query: 1534 YFPWHFFSPGSQYGPSGDSEFVVKSMKEMVKKLHANRIEVLLEVVFTHTAEVAALRQIDN 1355
             FP HFFSP S YGPSG +   + +MKEMVKKLHA+ IEVLLEVVFTH+AE  AL+ ID+
Sbjct: 374  CFPCHFFSPTSLYGPSGGAVSAINTMKEMVKKLHASGIEVLLEVVFTHSAESGALQGIDD 433

Query: 1354 SSYYHVEGGEDLESRNALNCNYPIVQQLILDSLRHWVIEYHIDGFCFINASSLMKGFHGE 1175
            SSYY ++G  DLE+RN LNCNYPIVQQ ILDSLR+WV E+H+DGFCFINAS L++G  GE
Sbjct: 434  SSYY-LKGVADLEARNVLNCNYPIVQQFILDSLRYWVTEFHVDGFCFINASLLLQGSRGE 492

Query: 1174 FLSRPPLVEAIAFDPLLSKVKIIADSWDPHNIEKKDISFPHWKRWAEINSKFCNDVRNFL 995
            +LS PPLVEAIAFDPLLSK KIIAD WDPHN   ++  FPHWK WAE+N KFCNDVRNFL
Sbjct: 493  YLSHPPLVEAIAFDPLLSKTKIIADCWDPHNTVLEETRFPHWKTWAEMNMKFCNDVRNFL 552

Query: 994  RGQGSLGNLATRLCGSGDLFLDGRGPAFSFNFISRNAGLPLVDXXXXXXXXXXXXXSWNC 815
            RG+G L +LATRLCGSGD+F  GRGPAFSFNFISRN+GLPLVD             SWNC
Sbjct: 553  RGEGLLSSLATRLCGSGDIFSKGRGPAFSFNFISRNSGLPLVDLVSFSSDKLASELSWNC 612

Query: 814  GEEGPTNKTAVLERRLKQIRNYLLILFISLGVPILNMGDECGQSSGGSPAYADQKPLDWN 635
            GEEGPTNKT VLERRLKQ RN+L +L++SLGVP+LNMGDECGQS+GGSPAY+D+K  DWN
Sbjct: 613  GEEGPTNKTLVLERRLKQTRNFLFVLYVSLGVPVLNMGDECGQSTGGSPAYSDRKSFDWN 672

Query: 634  ALKSGFAIQTTKFISFLSSLKMRRSDLLQNRNFLREENIVWHGSDGSPPRWDGPSYRFLA 455
            ALKS F IQTT+FISFLSSL+ RRSDLLQN N+L+EENI WHGSD SPPRW+ P+ +FLA
Sbjct: 673  ALKSSFGIQTTEFISFLSSLRRRRSDLLQNMNYLKEENIDWHGSDQSPPRWEDPTCKFLA 732

Query: 454  MTLKADSE----ESQSTSGSPEMIGDLFVAFNSAGHSENVALPPLTVDAAWFRLVDTSLP 287
            M L+ D +    E+Q+TSG     GDLF+AFN+A  SE+V LPP+    AW RLVDT+LP
Sbjct: 733  MRLRVDEDKDKAENQTTSGK----GDLFIAFNAADLSESVILPPIAEGMAWHRLVDTALP 788

Query: 286  FPGFFTTDGVPLK---DGSVAYEMKSHSCVLFEARS 188
            FPGFF TDG P+    DG +AYEMKS S  LFEARS
Sbjct: 789  FPGFFLTDGEPVPENVDGLLAYEMKSLSSTLFEARS 824


>ref|XP_002533079.1| isoamylase, putative [Ricinus communis] gi|223527143|gb|EEF29318.1|
            isoamylase, putative [Ricinus communis]
          Length = 872

 Score =  962 bits (2488), Expect = 0.0
 Identities = 479/753 (63%), Positives = 579/753 (76%), Gaps = 15/753 (1%)
 Frame = -2

Query: 2407 LSSLHSGR-DRDLVMSWGLFRSDSESFMPMDFQNSNADSKYSTIETPFVKDSSGRLVVEL 2231
            +SSL  G  D  L++ WG++RSDS  FMP+D QN   +++   ++T  V++S G   +EL
Sbjct: 119  VSSLELGTTDYRLMLIWGIYRSDSSCFMPLDSQNFAPNAR--KMDTALVQNSFGTFALEL 176

Query: 2230 DFDESLAPFYVSILLKS-LGSNLESSEIRSHRKTNFVVPVGFRLGRPAPLGLSVLANGSM 2054
            +F+    PFY+S LLKS L ++    EI++H+  NF VP+GF  G P+PLGLS   +GSM
Sbjct: 177  EFEPKQTPFYLSFLLKSKLNTDASGLEIKNHKNANFCVPIGFNSGDPSPLGLSFSTDGSM 236

Query: 2053 NFAFFSRHAESVVLCLYADTSANKPALEIDLDPYVNRSGDIWHALLDDSLPFVSYGYRCK 1874
            NFAFFSR+ E +VLCLY D++ +KPALE+DLDPYVNR+GD+WHA L+ +  F SYGYRCK
Sbjct: 237  NFAFFSRNVEGLVLCLYDDSTTDKPALELDLDPYVNRTGDVWHASLEGAWTFTSYGYRCK 296

Query: 1873 S-----NADK-KRQYVLLDPYAKVIE----DFGSGLPRKSVGKLWKEPGFDWSGDICPSL 1724
                  N  K   + VLLDPYA+VI     D GS L  K +G+L +EP F+W  DI P+L
Sbjct: 297  GAILQGNTSKVDMECVLLDPYARVIASSMTDHGSRLSAKYLGRLCEEPAFEWGSDIRPNL 356

Query: 1723 PMEKLIIYRLNVTRFTKDKSSKLPGDVVGTFAGISEKLQHFKDLGVNAILLEPIFPFDEQ 1544
             MEKLI+YRLNV RFT+ KS KL  D+ GTFAG+ EK+ HF++LGVNA+LLEPIFPFDEQ
Sbjct: 357  AMEKLIVYRLNVKRFTEHKSGKLYSDIAGTFAGLIEKMDHFRNLGVNAVLLEPIFPFDEQ 416

Query: 1543 KGPYFPWHFFSPGSQYGPSGDSEFVVKSMKEMVKKLHANRIEVLLEVVFTHTAEVAALRQ 1364
            KGPYFP+HFFSP + YGPSG S   + SMKEMVK+LHANRIEVLLEVVFTHTAE  AL+ 
Sbjct: 417  KGPYFPYHFFSPSNIYGPSGGSISAITSMKEMVKELHANRIEVLLEVVFTHTAEGGALQG 476

Query: 1363 IDNSSYYHVEGGEDLESRNALNCNYPIVQQLILDSLRHWVIEYHIDGFCFINASSLMKGF 1184
            ID+ SYY+ +   D  SRNALNCNYPIVQ++ILDSL+HWV E+HIDGFCFINAS+L+ GF
Sbjct: 477  IDDFSYYYTKSSMD--SRNALNCNYPIVQRMILDSLQHWVTEFHIDGFCFINASALLTGF 534

Query: 1183 HGEFLSRPPLVEAIAFDPLLSKVKIIADSWDPHNIEKKDISFPHWKRWAEINSKFCNDVR 1004
            HGE LSRPPLVEAIAFDP+LSK KIIAD W P +   K+  FPHWKRWAEIN KFC DVR
Sbjct: 535  HGEHLSRPPLVEAIAFDPILSKTKIIADPWHPEHRIPKETCFPHWKRWAEINPKFCIDVR 594

Query: 1003 NFLRGQGSLGNLATRLCGSGDLFLDGRGPAFSFNFISRNAGLPLVDXXXXXXXXXXXXXS 824
            NFLRG+  LG+LATRLCGSGD+F +GRGPAFSFN+I+RN+GLPLVD             S
Sbjct: 595  NFLRGESLLGDLATRLCGSGDIFSNGRGPAFSFNYIARNSGLPLVDLVSFSGGELGSELS 654

Query: 823  WNCGEEGPTNKTAVLERRLKQIRNYLLILFISLGVPILNMGDECGQSSGGSPAYADQKPL 644
            WNCGEEGPTNKTAVLERRLKQIRNYL IL++SLGVP+LNMGDECGQSS GS +Y D+KP 
Sbjct: 655  WNCGEEGPTNKTAVLERRLKQIRNYLFILYVSLGVPVLNMGDECGQSSRGSISYGDRKPF 714

Query: 643  DWNALKSGFAIQTTKFISFLSSLKMRRSDLLQNRNFLREENIVWHGSDGSPPRWDGPSYR 464
            DWNAL + F  Q T+FISFLSSL+MRRSDLLQ RNFL+EENI WHG+D SPPRW+ P+ +
Sbjct: 715  DWNALSTSFGNQMTQFISFLSSLRMRRSDLLQKRNFLKEENIDWHGNDQSPPRWEDPTCK 774

Query: 463  FLAMTLKADSEESQSTSGSPEMIGDLFVAFNSAGHSENVALPPLTVDAAWFRLVDTSLPF 284
            FLAMTLK D  ESQ +S    + GDLF+AFN+AGH+E+V LPP+     W RLVDT+LPF
Sbjct: 775  FLAMTLKIDKAESQLSSEPSNIKGDLFMAFNAAGHAESVILPPVPEGMIWRRLVDTALPF 834

Query: 283  PGFFTTDGVPLKD---GSVAYEMKSHSCVLFEA 194
            PGFF+ DG P+ +   G +AY+M SHSC LFEA
Sbjct: 835  PGFFSEDGEPVVEQIAGLIAYKMNSHSCTLFEA 867


>ref|XP_008368864.1| PREDICTED: isoamylase 2, chloroplastic-like [Malus domestica]
          Length = 870

 Score =  960 bits (2481), Expect = 0.0
 Identities = 481/748 (64%), Positives = 578/748 (77%), Gaps = 7/748 (0%)
 Frame = -2

Query: 2407 LSSLHSGR-DRDLVMSWGLFRSDSESFMPMDFQNSNADSKYSTIETPFVKDSSGRLVVEL 2231
            +S LH  R DR LV+SWG++R+DS SF+P +F++S    + + +ETPF   SSG+  +EL
Sbjct: 129  VSLLHLSRSDRRLVLSWGMYRADSSSFVPSNFRSSTPADRTTALETPFTGTSSGKFTLEL 188

Query: 2230 DFDESLAPFYVSILLKS-LGSNLESSEIRSHRKTNFVVPVGFRLGRPAPLGLSVLANGSM 2054
            +F+    PFY+S +LKS   ++L   EIRSHRKTNF  PVGF  G PAPLG+S   +GSM
Sbjct: 189  EFEAKQIPFYLSFVLKSPADADLSDLEIRSHRKTNFCFPVGFGRGCPAPLGVSFSNDGSM 248

Query: 2053 NFAFFSRHAESVVLCLYADTSANKPALEIDLDPYVNRSGDIWHALLDDSLPFVSYGYRCK 1874
            NFA FSR+AESVVLCLYA+T+A KP LE+DLDPYVNRSGDIWHA  +++  FVSYGYR  
Sbjct: 249  NFAIFSRNAESVVLCLYAETTAEKPVLELDLDPYVNRSGDIWHASFENAWDFVSYGYRFD 308

Query: 1873 SNADKKRQYVLLDPYAKVI-EDFGSGLPRKSVGKLWKEPGFDWSGDICPSLPMEKLIIYR 1697
                     VLLDPYAK+I +    G   K +G+L +EP F+W+GD+ P L +EKL++YR
Sbjct: 309  EGN------VLLDPYAKIIAKSVPHGTGSKYLGRLCEEPAFEWAGDVXPDLTLEKLVVYR 362

Query: 1696 LNVTRFTKDKSSKLPGDVVGTFAGISEKLQHFKDLGVNAILLEPIFPFDEQKGPYFPWHF 1517
            LNV RFTK KSSKLP ++ GTF+G++EKL+HFK LGVNA+LLEPIFPFDEQKGPYFP HF
Sbjct: 363  LNVKRFTKHKSSKLPTNIAGTFSGLTEKLEHFKALGVNAVLLEPIFPFDEQKGPYFPVHF 422

Query: 1516 FSPGSQYGPSGDSEFVVKSMKEMVKKLHANRIEVLLEVVFTHTAEVAALRQIDNSSYYHV 1337
            FSP + +GP       V SMKEMVK  HA+ IEVLLEVVFTHT+E  A++ ID SSYYHV
Sbjct: 423  FSPMNCFGPPRGPVSAVISMKEMVKXFHADGIEVLLEVVFTHTSEGEAMQGIDISSYYHV 482

Query: 1336 EGGEDLESRNALNCNYPIVQQLILDSLRHWVIEYHIDGFCFINASSLMKGFHGEFLSRPP 1157
             G EDLE+RNALNCNYP+VQQ++LDSL +WV E+H+DGFCFINASSL++G +GE+LSRPP
Sbjct: 483  NGVEDLEARNALNCNYPVVQQMVLDSLXYWVTEFHVDGFCFINASSLLRGSNGEYLSRPP 542

Query: 1156 LVEAIAFDPLLSKVKIIADSWDPHNIEKKDISFPHWKRWAEINSKFCNDVRNFLRGQGSL 977
            LVEAIAFDPLLSK KIIAD WDP  +  K+  FPHWKRWAE+N+KF NDVRNFLRG+G L
Sbjct: 543  LVEAIAFDPLLSKTKIIADCWDPRGLVPKETRFPHWKRWAEVNTKFSNDVRNFLRGEGLL 602

Query: 976  GNLATRLCGSGDLFLDGRGPAFSFNFISRNAGLPLVDXXXXXXXXXXXXXSWNCGEEGPT 797
             +LATRLCG+GD+  DGRGPA SFNFISRN+GL LVD             S NCGEEGPT
Sbjct: 603  SDLATRLCGNGDIXSDGRGPASSFNFISRNSGLXLVDLVSFSGVELAAELSXNCGEEGPT 662

Query: 796  NKTAVLERRLKQIRNYLLILFISLGVPILNMGDECGQSSGGSPAYADQKPLDWNALKSGF 617
            NKTAVLERRLKQIRN+L ILF+SLGVP+LNMGDE GQS+GGSPAY+D+K  DWNAL +GF
Sbjct: 663  NKTAVLERRLKQIRNFLFILFVSLGVPVLNMGDEYGQSAGGSPAYSDRKAFDWNALGTGF 722

Query: 616  AIQTTKFISFLSSLKMRRSDLLQNRNFLREENIVWHGSDGSPPRWDGPSYRFLAMTLKAD 437
            A QTT+FI+FLSS ++RRSD LQ RNFL+EENI W+GS  S P+W+ PS +FLAM LKAD
Sbjct: 723  ATQTTQFIAFLSSFRIRRSDXLQKRNFLKEENIDWYGSXQSSPKWEDPSXKFLAMKLKAD 782

Query: 436  SEES-QSTSGSPEMIGDLFVAFNSAGHSENVALPPLTVDAAWFRLVDTSLPFPGFFTTDG 260
             EE+ +S   S    GDLFVAF++A  SE V LPPL     WF LVDT+LPFPGFF+TDG
Sbjct: 783  XEEAYESGDDSSPSWGDLFVAFSAADJSETVILPPLPEGXGWFXLVDTALPFPGFFSTDG 842

Query: 259  VPLKD---GSVAYEMKSHSCVLFEARSL 185
             P+ +   G  AYEM SHSC LFEARSL
Sbjct: 843  EPVAEQPAGLFAYEMXSHSCALFEARSL 870


>ref|XP_012092290.1| PREDICTED: isoamylase 2, chloroplastic [Jatropha curcas]
            gi|643704433|gb|KDP21497.1| hypothetical protein
            JCGZ_21968 [Jatropha curcas]
          Length = 865

 Score =  959 bits (2479), Expect = 0.0
 Identities = 472/743 (63%), Positives = 569/743 (76%), Gaps = 8/743 (1%)
 Frame = -2

Query: 2392 SGRDRDLVMSWGLFRSDSESFMPMDFQNSNADSKYSTIETPFVKDSSGRLVVELDFDESL 2213
            S RD  L + WG++RSDS  FMP+D Q+ + D++  T++TPFV++S GR  +EL+F+   
Sbjct: 134  STRDYKLTLIWGIYRSDSACFMPLDSQHLDPDAR--TMDTPFVQNSFGRFALELEFEAKQ 191

Query: 2212 APFYVSILLKSL-GSNLESSEIRSHRKTNFVVPVGFRLGRPAPLGLSVLANGSMNFAFFS 2036
             P Y+S LLKS+  S+  S EIR+HR+TNF VP+GF  G P PLGLS   +GSMNFAFFS
Sbjct: 192  TPIYLSFLLKSMFNSDSSSLEIRNHRQTNFCVPIGFSSGYPNPLGLSFSNDGSMNFAFFS 251

Query: 2035 RHAESVVLCLYADTSANKPALEIDLDPYVNRSGDIWHALLDDSLPFVSYGYRCKSNADKK 1856
            R+AE VVLCLY D++ +KPALE+DLDPYVNRSGD+WHA L+ +  F SYGYRCK      
Sbjct: 252  RNAEGVVLCLYDDSTTDKPALELDLDPYVNRSGDVWHASLESAWTFTSYGYRCKGTGKVD 311

Query: 1855 RQYVLLDPYAKVI----EDFGSGLPRKSVGKLWKEPGFDWSGDICPSLPMEKLIIYRLNV 1688
             + VLLDPYA++I     D GSGL  K +G+L + P F+W  D+ P+L MEKL++YRLNV
Sbjct: 312  VERVLLDPYARIIVNCTADDGSGLSTKYLGRLCEVPAFEWGDDVRPNLAMEKLVVYRLNV 371

Query: 1687 TRFTKDKSSKLPGDVVGTFAGISEKLQHFKDLGVNAILLEPIFPFDEQKGPYFPWHFFSP 1508
              FT+ KSSKL  D+ GTFAG++EKL HFK+LGVNA+LLEPIFPFDE KGPYFP HFFSP
Sbjct: 372  KHFTESKSSKLYSDIDGTFAGLTEKLNHFKNLGVNAVLLEPIFPFDEGKGPYFPCHFFSP 431

Query: 1507 GSQYGPSGDSEFVVKSMKEMVKKLHANRIEVLLEVVFTHTAEVAALRQIDNSSYYHVEGG 1328
             + YGPSG S   + SMKEMVK+LHAN +EVLLEVVFTHTAE  AL+ ID+ SYY+    
Sbjct: 432  SNLYGPSGGSISAITSMKEMVKELHANGVEVLLEVVFTHTAEAGALQGIDDFSYYYANRV 491

Query: 1327 EDLESRNALNCNYPIVQQLILDSLRHWVIEYHIDGFCFINASSLMKGFHGEFLSRPPLVE 1148
             DLESRNALNCNYPIVQ++ILDSLRHWV E+HIDGFCF+NAS L++GFHGE LSRPPLVE
Sbjct: 492  VDLESRNALNCNYPIVQRMILDSLRHWVTEFHIDGFCFMNASFLLRGFHGEILSRPPLVE 551

Query: 1147 AIAFDPLLSKVKIIADSWDPHNIEKKDISFPHWKRWAEINSKFCNDVRNFLRGQGSLGNL 968
            AIAFDPLLS  KIIAD WDP ++  ++I  PHWKRWAEIN+KF +DVRNFLRG+  L +L
Sbjct: 552  AIAFDPLLSNTKIIADCWDPEDMTPEEICLPHWKRWAEINTKFRSDVRNFLRGESLLSDL 611

Query: 967  ATRLCGSGDLFLDGRGPAFSFNFISRNAGLPLVDXXXXXXXXXXXXXSWNCGEEGPTNKT 788
            ATRLCGSGD+F  GRGPAFSFNFI+RN+GLPLVD              WNCGEEGPTNK 
Sbjct: 612  ATRLCGSGDIFSSGRGPAFSFNFIARNSGLPLVDLVSFSSDELASELCWNCGEEGPTNKI 671

Query: 787  AVLERRLKQIRNYLLILFISLGVPILNMGDECGQSSGGSPAYADQKPLDWNALKSGFAIQ 608
             VLERRLKQIRNYL IL++SLGVP+LNMGDECGQSSGGS +Y D+KP DWN+L +GF IQ
Sbjct: 672  PVLERRLKQIRNYLFILYVSLGVPVLNMGDECGQSSGGSISYGDRKPFDWNSLSTGFGIQ 731

Query: 607  TTKFISFLSSLKMRRSDLLQNRNFLREENIVWHGSDGSPPRWDGPSYRFLAMTLKADSEE 428
             T+FISFLSSL+ R SD+LQ RNFL+EENI W+G+D SPPRW+ PS +FLAMTL+  S  
Sbjct: 732  MTQFISFLSSLRTRWSDVLQKRNFLKEENIDWYGTDQSPPRWEDPSCKFLAMTLRTKSS- 790

Query: 427  SQSTSGSPEMIGDLFVAFNSAGHSENVALPPLTVDAAWFRLVDTSLPFPGFFTTDGVPLK 248
                       G+LF+AFN+A  SE+V LP L     W RLVDT+LPFPGFF+ DG P+ 
Sbjct: 791  -----------GNLFMAFNAADQSESVILPQLPEGMTWLRLVDTALPFPGFFSNDGEPVV 839

Query: 247  DGS---VAYEMKSHSCVLFEARS 188
            +     + Y MKSHSC+LFEARS
Sbjct: 840  EQMAELIVYGMKSHSCILFEARS 862