BLASTX nr result
ID: Forsythia22_contig00052442
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Forsythia22_contig00052442
(313 letters)
Database: ./nr
69,698,275 sequences; 24,982,196,650 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_011088450.1| PREDICTED: SNF2 domain-containing protein CL... 180 4e-43
emb|CDO97016.1| unnamed protein product [Coffea canephora] 179 8e-43
ref|XP_012837075.1| PREDICTED: SNF2 domain-containing protein CL... 173 3e-41
ref|XP_010650785.1| PREDICTED: SNF2 domain-containing protein CL... 173 3e-41
ref|XP_002275596.1| PREDICTED: SNF2 domain-containing protein CL... 173 3e-41
gb|EYU37828.1| hypothetical protein MIMGU_mgv1a000946mg [Erythra... 173 3e-41
ref|XP_002273814.2| PREDICTED: SNF2 domain-containing protein CL... 173 5e-41
gb|EPS74542.1| hypothetical protein M569_00205, partial [Genlise... 173 5e-41
emb|CAN62867.1| hypothetical protein VITISV_015925 [Vitis vinifera] 173 5e-41
ref|XP_011037462.1| PREDICTED: SNF2 domain-containing protein CL... 167 3e-39
ref|XP_011037461.1| PREDICTED: SNF2 domain-containing protein CL... 167 3e-39
ref|XP_011037161.1| PREDICTED: SNF2 domain-containing protein CL... 167 3e-39
ref|XP_007133805.1| hypothetical protein PHAVU_011G210600g [Phas... 167 3e-39
gb|KHN19463.1| DNA repair protein rhp54 [Glycine soja] 166 4e-39
ref|XP_006592957.1| PREDICTED: SNF2 domain-containing protein CL... 166 4e-39
ref|XP_003540522.1| PREDICTED: SNF2 domain-containing protein CL... 166 4e-39
ref|XP_007133808.1| hypothetical protein PHAVU_011G210800g [Phas... 166 6e-39
ref|XP_011047795.1| PREDICTED: SNF2 domain-containing protein CL... 166 7e-39
ref|XP_009757870.1| PREDICTED: SNF2 domain-containing protein CL... 166 7e-39
ref|XP_009757869.1| PREDICTED: SNF2 domain-containing protein CL... 166 7e-39
>ref|XP_011088450.1| PREDICTED: SNF2 domain-containing protein CLASSY 3-like [Sesamum
indicum]
Length = 927
Score = 180 bits (456), Expect = 4e-43
Identities = 86/104 (82%), Positives = 93/104 (89%)
Frame = -1
Query: 313 GKTFMIISFLQSFMAKYPFARPLVVLPRGILATWKKEFLRWQVEGWPLYDFYSVKADSRS 134
GKTFMIISFLQSFMAKYP ARPLVVLPRGILA WKKEFLRWQVEG PLYDFYSVKADSR+
Sbjct: 417 GKTFMIISFLQSFMAKYPGARPLVVLPRGILAIWKKEFLRWQVEGIPLYDFYSVKADSRA 476
Query: 133 QQLQVLSDWAKERSILFLGYKQFSSIVCDRGSGKAAISCQEILL 2
QQL+VL +W KERSILFLGYKQFSSI+CD G+ A++CQ LL
Sbjct: 477 QQLEVLKEWVKERSILFLGYKQFSSIICDTDDGQVAVACQNYLL 520
>emb|CDO97016.1| unnamed protein product [Coffea canephora]
Length = 906
Score = 179 bits (453), Expect = 8e-43
Identities = 88/104 (84%), Positives = 93/104 (89%)
Frame = -1
Query: 313 GKTFMIISFLQSFMAKYPFARPLVVLPRGILATWKKEFLRWQVEGWPLYDFYSVKADSRS 134
GKTFMIISFLQSFMAKYPF+RPLVVLPRGILATWKKEF RWQVE PL+DFYSVKADSR+
Sbjct: 397 GKTFMIISFLQSFMAKYPFSRPLVVLPRGILATWKKEFQRWQVEDIPLFDFYSVKADSRT 456
Query: 133 QQLQVLSDWAKERSILFLGYKQFSSIVCDRGSGKAAISCQEILL 2
QQL+VL WA+E SILFLGYKQFSSIVCD KAA SCQEILL
Sbjct: 457 QQLEVLRKWAEEMSILFLGYKQFSSIVCDTNCSKAAASCQEILL 500
>ref|XP_012837075.1| PREDICTED: SNF2 domain-containing protein CLASSY 3-like isoform X1
[Erythranthe guttatus] gi|848873036|ref|XP_012837076.1|
PREDICTED: SNF2 domain-containing protein CLASSY 3-like
isoform X2 [Erythranthe guttatus]
Length = 966
Score = 173 bits (439), Expect = 3e-41
Identities = 83/104 (79%), Positives = 89/104 (85%)
Frame = -1
Query: 313 GKTFMIISFLQSFMAKYPFARPLVVLPRGILATWKKEFLRWQVEGWPLYDFYSVKADSRS 134
GKTFMIISFLQSFMAKYP ARPLVVLPRGIL WK+EF RWQVE PLYDFYSVKADSRS
Sbjct: 456 GKTFMIISFLQSFMAKYPGARPLVVLPRGILMIWKREFTRWQVEDIPLYDFYSVKADSRS 515
Query: 133 QQLQVLSDWAKERSILFLGYKQFSSIVCDRGSGKAAISCQEILL 2
QQL+VL W KERS+LFLGYKQFSSIVCD+ GK ++CQ LL
Sbjct: 516 QQLEVLKQWVKERSVLFLGYKQFSSIVCDKDDGKVTLACQNYLL 559
>ref|XP_010650785.1| PREDICTED: SNF2 domain-containing protein CLASSY 2-like isoform X2
[Vitis vinifera] gi|731391494|ref|XP_010650786.1|
PREDICTED: SNF2 domain-containing protein CLASSY 2-like
isoform X2 [Vitis vinifera]
Length = 904
Score = 173 bits (439), Expect = 3e-41
Identities = 84/104 (80%), Positives = 92/104 (88%)
Frame = -1
Query: 313 GKTFMIISFLQSFMAKYPFARPLVVLPRGILATWKKEFLRWQVEGWPLYDFYSVKADSRS 134
GKTFMIISF+QSF+AKYP ARPLVVLP+GILATWKKEFL WQVE PLYDFYSVKADSR
Sbjct: 390 GKTFMIISFMQSFLAKYPQARPLVVLPKGILATWKKEFLTWQVEDIPLYDFYSVKADSRP 449
Query: 133 QQLQVLSDWAKERSILFLGYKQFSSIVCDRGSGKAAISCQEILL 2
QQL+VL W E+SILFLGYKQFSSIVC G+ KAA++CQEILL
Sbjct: 450 QQLEVLKQWVAEKSILFLGYKQFSSIVCGDGASKAAMACQEILL 493
>ref|XP_002275596.1| PREDICTED: SNF2 domain-containing protein CLASSY 2-like isoform X1
[Vitis vinifera]
Length = 944
Score = 173 bits (439), Expect = 3e-41
Identities = 84/104 (80%), Positives = 92/104 (88%)
Frame = -1
Query: 313 GKTFMIISFLQSFMAKYPFARPLVVLPRGILATWKKEFLRWQVEGWPLYDFYSVKADSRS 134
GKTFMIISF+QSF+AKYP ARPLVVLP+GILATWKKEFL WQVE PLYDFYSVKADSR
Sbjct: 430 GKTFMIISFMQSFLAKYPQARPLVVLPKGILATWKKEFLTWQVEDIPLYDFYSVKADSRP 489
Query: 133 QQLQVLSDWAKERSILFLGYKQFSSIVCDRGSGKAAISCQEILL 2
QQL+VL W E+SILFLGYKQFSSIVC G+ KAA++CQEILL
Sbjct: 490 QQLEVLKQWVAEKSILFLGYKQFSSIVCGDGASKAAMACQEILL 533
>gb|EYU37828.1| hypothetical protein MIMGU_mgv1a000946mg [Erythranthe guttata]
Length = 936
Score = 173 bits (439), Expect = 3e-41
Identities = 83/104 (79%), Positives = 89/104 (85%)
Frame = -1
Query: 313 GKTFMIISFLQSFMAKYPFARPLVVLPRGILATWKKEFLRWQVEGWPLYDFYSVKADSRS 134
GKTFMIISFLQSFMAKYP ARPLVVLPRGIL WK+EF RWQVE PLYDFYSVKADSRS
Sbjct: 426 GKTFMIISFLQSFMAKYPGARPLVVLPRGILMIWKREFTRWQVEDIPLYDFYSVKADSRS 485
Query: 133 QQLQVLSDWAKERSILFLGYKQFSSIVCDRGSGKAAISCQEILL 2
QQL+VL W KERS+LFLGYKQFSSIVCD+ GK ++CQ LL
Sbjct: 486 QQLEVLKQWVKERSVLFLGYKQFSSIVCDKDDGKVTLACQNYLL 529
>ref|XP_002273814.2| PREDICTED: SNF2 domain-containing protein CLASSY 2-like [Vitis
vinifera]
Length = 945
Score = 173 bits (438), Expect = 5e-41
Identities = 84/104 (80%), Positives = 91/104 (87%)
Frame = -1
Query: 313 GKTFMIISFLQSFMAKYPFARPLVVLPRGILATWKKEFLRWQVEGWPLYDFYSVKADSRS 134
GKTFMIISF+QSF+AKYP ARPLVVLP+GILATWKKEFL WQVE PLYDFYSVKADSR
Sbjct: 431 GKTFMIISFMQSFLAKYPQARPLVVLPKGILATWKKEFLTWQVEDIPLYDFYSVKADSRP 490
Query: 133 QQLQVLSDWAKERSILFLGYKQFSSIVCDRGSGKAAISCQEILL 2
QQL+VL W E+SILFLGYKQFSSIVC G+ KA I+CQEILL
Sbjct: 491 QQLEVLKQWVAEKSILFLGYKQFSSIVCGDGASKATIACQEILL 534
>gb|EPS74542.1| hypothetical protein M569_00205, partial [Genlisea aurea]
Length = 872
Score = 173 bits (438), Expect = 5e-41
Identities = 83/104 (79%), Positives = 90/104 (86%)
Frame = -1
Query: 313 GKTFMIISFLQSFMAKYPFARPLVVLPRGILATWKKEFLRWQVEGWPLYDFYSVKADSRS 134
GKTFMIISFLQSFMAKYP RPLVVLPRGIL WKKEF+RWQVE PLYDFYSVKADSR
Sbjct: 363 GKTFMIISFLQSFMAKYPGGRPLVVLPRGILPIWKKEFIRWQVEHIPLYDFYSVKADSRL 422
Query: 133 QQLQVLSDWAKERSILFLGYKQFSSIVCDRGSGKAAISCQEILL 2
QQL+VL +W KERSILFLGYKQFSSI+CD G+AA++CQ LL
Sbjct: 423 QQLEVLKEWVKERSILFLGYKQFSSIICDTDDGQAAVACQNYLL 466
>emb|CAN62867.1| hypothetical protein VITISV_015925 [Vitis vinifera]
Length = 1187
Score = 173 bits (438), Expect = 5e-41
Identities = 84/104 (80%), Positives = 91/104 (87%)
Frame = -1
Query: 313 GKTFMIISFLQSFMAKYPFARPLVVLPRGILATWKKEFLRWQVEGWPLYDFYSVKADSRS 134
GKTFMIISF+QSF+AKYP ARPLVVLP+GILATWKKEFL WQVE PLYDFYSVKADSR
Sbjct: 615 GKTFMIISFMQSFLAKYPQARPLVVLPKGILATWKKEFLTWQVEDIPLYDFYSVKADSRP 674
Query: 133 QQLQVLSDWAKERSILFLGYKQFSSIVCDRGSGKAAISCQEILL 2
QQL+VL W E+SILFLGYKQFSSIVC G+ KA I+CQEILL
Sbjct: 675 QQLEVLKQWVAEKSILFLGYKQFSSIVCGDGASKATIACQEILL 718
>ref|XP_011037462.1| PREDICTED: SNF2 domain-containing protein CLASSY 3-like isoform X2
[Populus euphratica]
Length = 705
Score = 167 bits (422), Expect = 3e-39
Identities = 76/104 (73%), Positives = 91/104 (87%)
Frame = -1
Query: 313 GKTFMIISFLQSFMAKYPFARPLVVLPRGILATWKKEFLRWQVEGWPLYDFYSVKADSRS 134
GKTFMIISF+QSF+AKYP A+PLVVLP+GIL TWK+EF WQ+E +PLYDFYSVKADSR
Sbjct: 201 GKTFMIISFMQSFLAKYPHAKPLVVLPKGILPTWKREFQIWQIEDFPLYDFYSVKADSRQ 260
Query: 133 QQLQVLSDWAKERSILFLGYKQFSSIVCDRGSGKAAISCQEILL 2
QQL+VL W +++SILFLGYKQFSSIVCD G + +++CQEILL
Sbjct: 261 QQLEVLKQWVEQKSILFLGYKQFSSIVCDDGKNQVSVTCQEILL 304
>ref|XP_011037461.1| PREDICTED: SNF2 domain-containing protein CLASSY 3-like isoform X1
[Populus euphratica]
Length = 859
Score = 167 bits (422), Expect = 3e-39
Identities = 76/104 (73%), Positives = 91/104 (87%)
Frame = -1
Query: 313 GKTFMIISFLQSFMAKYPFARPLVVLPRGILATWKKEFLRWQVEGWPLYDFYSVKADSRS 134
GKTFMIISF+QSF+AKYP A+PLVVLP+GIL TWK+EF WQ+E +PLYDFYSVKADSR
Sbjct: 355 GKTFMIISFMQSFLAKYPHAKPLVVLPKGILPTWKREFQIWQIEDFPLYDFYSVKADSRQ 414
Query: 133 QQLQVLSDWAKERSILFLGYKQFSSIVCDRGSGKAAISCQEILL 2
QQL+VL W +++SILFLGYKQFSSIVCD G + +++CQEILL
Sbjct: 415 QQLEVLKQWVEQKSILFLGYKQFSSIVCDDGKNQVSVTCQEILL 458
>ref|XP_011037161.1| PREDICTED: SNF2 domain-containing protein CLASSY 3-like [Populus
euphratica] gi|743883964|ref|XP_011037162.1| PREDICTED:
SNF2 domain-containing protein CLASSY 3-like [Populus
euphratica]
Length = 857
Score = 167 bits (422), Expect = 3e-39
Identities = 76/104 (73%), Positives = 91/104 (87%)
Frame = -1
Query: 313 GKTFMIISFLQSFMAKYPFARPLVVLPRGILATWKKEFLRWQVEGWPLYDFYSVKADSRS 134
GKTFMIISF+QSF+AKYP A+PLVVLP+GIL TWK+EF WQ+E +PLYDFYSVKADSR
Sbjct: 353 GKTFMIISFMQSFLAKYPHAKPLVVLPKGILPTWKREFQIWQIEDFPLYDFYSVKADSRQ 412
Query: 133 QQLQVLSDWAKERSILFLGYKQFSSIVCDRGSGKAAISCQEILL 2
QQL+VL W +++SILFLGYKQFSSIVCD G + +++CQEILL
Sbjct: 413 QQLEVLKQWVEQKSILFLGYKQFSSIVCDDGKNQVSVTCQEILL 456
>ref|XP_007133805.1| hypothetical protein PHAVU_011G210600g [Phaseolus vulgaris]
gi|593263254|ref|XP_007133806.1| hypothetical protein
PHAVU_011G210600g [Phaseolus vulgaris]
gi|561006805|gb|ESW05799.1| hypothetical protein
PHAVU_011G210600g [Phaseolus vulgaris]
gi|561006806|gb|ESW05800.1| hypothetical protein
PHAVU_011G210600g [Phaseolus vulgaris]
Length = 900
Score = 167 bits (422), Expect = 3e-39
Identities = 78/104 (75%), Positives = 91/104 (87%)
Frame = -1
Query: 313 GKTFMIISFLQSFMAKYPFARPLVVLPRGILATWKKEFLRWQVEGWPLYDFYSVKADSRS 134
GKTFMIISF+QSF+ KYP ARPLVVLP+GIL+TWKKEF WQVE PLYDFY+VKADSRS
Sbjct: 383 GKTFMIISFMQSFLGKYPNARPLVVLPKGILSTWKKEFQIWQVEDIPLYDFYTVKADSRS 442
Query: 133 QQLQVLSDWAKERSILFLGYKQFSSIVCDRGSGKAAISCQEILL 2
QQL+VL W +++SILFLGYKQFSSIVCD G+ ++SCQ+ILL
Sbjct: 443 QQLEVLKQWMEQKSILFLGYKQFSSIVCDNGTNSTSLSCQDILL 486
>gb|KHN19463.1| DNA repair protein rhp54 [Glycine soja]
Length = 898
Score = 166 bits (421), Expect = 4e-39
Identities = 78/104 (75%), Positives = 90/104 (86%)
Frame = -1
Query: 313 GKTFMIISFLQSFMAKYPFARPLVVLPRGILATWKKEFLRWQVEGWPLYDFYSVKADSRS 134
GKTFMIISF+QSF+ KYP ARPLVVLP+GIL+TWKKEF WQVE PLYD Y+VKADSRS
Sbjct: 381 GKTFMIISFMQSFLGKYPNARPLVVLPKGILSTWKKEFQTWQVEDIPLYDLYTVKADSRS 440
Query: 133 QQLQVLSDWAKERSILFLGYKQFSSIVCDRGSGKAAISCQEILL 2
QQL+VL W +++SILFLGYKQFSSIVCD G+ ++SCQEILL
Sbjct: 441 QQLEVLKQWMEQKSILFLGYKQFSSIVCDNGTNNTSLSCQEILL 484
>ref|XP_006592957.1| PREDICTED: SNF2 domain-containing protein CLASSY 1-like isoform X3
[Glycine max]
Length = 881
Score = 166 bits (421), Expect = 4e-39
Identities = 78/104 (75%), Positives = 90/104 (86%)
Frame = -1
Query: 313 GKTFMIISFLQSFMAKYPFARPLVVLPRGILATWKKEFLRWQVEGWPLYDFYSVKADSRS 134
GKTFMIISF+QSF+ KYP ARPLVVLP+GIL+TWKKEF WQVE PLYD Y+VKADSRS
Sbjct: 381 GKTFMIISFMQSFLGKYPNARPLVVLPKGILSTWKKEFQTWQVEDIPLYDLYTVKADSRS 440
Query: 133 QQLQVLSDWAKERSILFLGYKQFSSIVCDRGSGKAAISCQEILL 2
QQL+VL W +++SILFLGYKQFSSIVCD G+ ++SCQEILL
Sbjct: 441 QQLEVLKQWMEQKSILFLGYKQFSSIVCDNGTNNTSLSCQEILL 484
>ref|XP_003540522.1| PREDICTED: SNF2 domain-containing protein CLASSY 1-like isoform X1
[Glycine max] gi|571494837|ref|XP_006592956.1|
PREDICTED: SNF2 domain-containing protein CLASSY 1-like
isoform X2 [Glycine max]
Length = 883
Score = 166 bits (421), Expect = 4e-39
Identities = 78/104 (75%), Positives = 90/104 (86%)
Frame = -1
Query: 313 GKTFMIISFLQSFMAKYPFARPLVVLPRGILATWKKEFLRWQVEGWPLYDFYSVKADSRS 134
GKTFMIISF+QSF+ KYP ARPLVVLP+GIL+TWKKEF WQVE PLYD Y+VKADSRS
Sbjct: 383 GKTFMIISFMQSFLGKYPNARPLVVLPKGILSTWKKEFQTWQVEDIPLYDLYTVKADSRS 442
Query: 133 QQLQVLSDWAKERSILFLGYKQFSSIVCDRGSGKAAISCQEILL 2
QQL+VL W +++SILFLGYKQFSSIVCD G+ ++SCQEILL
Sbjct: 443 QQLEVLKQWMEQKSILFLGYKQFSSIVCDNGTNNTSLSCQEILL 486
>ref|XP_007133808.1| hypothetical protein PHAVU_011G210800g [Phaseolus vulgaris]
gi|561006808|gb|ESW05802.1| hypothetical protein
PHAVU_011G210800g [Phaseolus vulgaris]
Length = 871
Score = 166 bits (420), Expect = 6e-39
Identities = 77/104 (74%), Positives = 91/104 (87%)
Frame = -1
Query: 313 GKTFMIISFLQSFMAKYPFARPLVVLPRGILATWKKEFLRWQVEGWPLYDFYSVKADSRS 134
GKTFMIISF+QSF+ KYP ARPLVVLP+GIL+TWKKEF WQVE PLYDFY+VKADSRS
Sbjct: 373 GKTFMIISFMQSFLGKYPNARPLVVLPKGILSTWKKEFQIWQVEDIPLYDFYTVKADSRS 432
Query: 133 QQLQVLSDWAKERSILFLGYKQFSSIVCDRGSGKAAISCQEILL 2
QQL+VL W +++S+LFLGYKQFSSIVCD G+ ++SCQ+ILL
Sbjct: 433 QQLEVLKQWMEKKSVLFLGYKQFSSIVCDNGTNSTSLSCQDILL 476
>ref|XP_011047795.1| PREDICTED: SNF2 domain-containing protein CLASSY 3 [Populus
euphratica]
Length = 859
Score = 166 bits (419), Expect = 7e-39
Identities = 77/104 (74%), Positives = 90/104 (86%)
Frame = -1
Query: 313 GKTFMIISFLQSFMAKYPFARPLVVLPRGILATWKKEFLRWQVEGWPLYDFYSVKADSRS 134
GKTFMIISF+QSF+AKYP A+PLVVLP+GIL+TWKKEF WQ+E PLYDFYSVKADSR
Sbjct: 354 GKTFMIISFMQSFLAKYPHAKPLVVLPKGILSTWKKEFQIWQIEDIPLYDFYSVKADSRH 413
Query: 133 QQLQVLSDWAKERSILFLGYKQFSSIVCDRGSGKAAISCQEILL 2
QQL+VL W + +SILFLGYKQFSSIVCD G + +++CQEILL
Sbjct: 414 QQLEVLKQWLEHKSILFLGYKQFSSIVCDDGKNQVSVTCQEILL 457
>ref|XP_009757870.1| PREDICTED: SNF2 domain-containing protein CLASSY 3 isoform X2
[Nicotiana sylvestris]
Length = 925
Score = 166 bits (419), Expect = 7e-39
Identities = 82/104 (78%), Positives = 89/104 (85%)
Frame = -1
Query: 313 GKTFMIISFLQSFMAKYPFARPLVVLPRGILATWKKEFLRWQVEGWPLYDFYSVKADSRS 134
GKTFMIISFLQSFMA ARPLVVLPRGILATWKKEFLRWQV+ PLYDFYSVKAD+RS
Sbjct: 416 GKTFMIISFLQSFMANNDRARPLVVLPRGILATWKKEFLRWQVDEIPLYDFYSVKADNRS 475
Query: 133 QQLQVLSDWAKERSILFLGYKQFSSIVCDRGSGKAAISCQEILL 2
QQL+VL W++ERSILFLGYKQFS+IVCD A +CQEILL
Sbjct: 476 QQLEVLKQWSQERSILFLGYKQFSTIVCDNVGSTTAAACQEILL 519
>ref|XP_009757869.1| PREDICTED: SNF2 domain-containing protein CLASSY 3 isoform X1
[Nicotiana sylvestris]
Length = 927
Score = 166 bits (419), Expect = 7e-39
Identities = 82/104 (78%), Positives = 89/104 (85%)
Frame = -1
Query: 313 GKTFMIISFLQSFMAKYPFARPLVVLPRGILATWKKEFLRWQVEGWPLYDFYSVKADSRS 134
GKTFMIISFLQSFMA ARPLVVLPRGILATWKKEFLRWQV+ PLYDFYSVKAD+RS
Sbjct: 418 GKTFMIISFLQSFMANNDRARPLVVLPRGILATWKKEFLRWQVDEIPLYDFYSVKADNRS 477
Query: 133 QQLQVLSDWAKERSILFLGYKQFSSIVCDRGSGKAAISCQEILL 2
QQL+VL W++ERSILFLGYKQFS+IVCD A +CQEILL
Sbjct: 478 QQLEVLKQWSQERSILFLGYKQFSTIVCDNVGSTTAAACQEILL 521