BLASTX nr result
ID: Forsythia22_contig00037887
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Forsythia22_contig00037887
(327 letters)
Database: ./nr
69,698,275 sequences; 24,982,196,650 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_012842534.1| PREDICTED: uncharacterized protein LOC105962... 130 3e-28
ref|XP_012851396.1| PREDICTED: uncharacterized protein LOC105971... 129 8e-28
emb|CAD40681.2| OSJNBb0118P14.3 [Oryza sativa Japonica Group] 128 1e-27
ref|NP_001050777.2| Os03g0648200 [Oryza sativa Japonica Group] g... 127 4e-27
gb|ABF97896.1| transposon protein, putative, Mutator sub-class [... 127 4e-27
gb|AAU89193.1| transposon protein, putative, mutator sub-class [... 127 4e-27
emb|CAH67772.1| H0322F07.9 [Oryza sativa Indica Group] gi|903991... 125 9e-27
gb|AAT77288.1| putative polyprotein [Oryza sativa Japonica Group] 125 9e-27
gb|EEC80682.1| hypothetical protein OsI_23105 [Oryza sativa Indi... 125 1e-26
gb|AAX93020.1| transposon protein, putative, mutator sub-class [... 124 2e-26
ref|XP_011083912.1| PREDICTED: uncharacterized protein LOC105166... 122 1e-25
gb|EEC80689.1| hypothetical protein OsI_23113 [Oryza sativa Indi... 121 2e-25
gb|AAK63883.1|AC074105_12 Putative transposon protein [Oryza sat... 121 2e-25
ref|XP_012840437.1| PREDICTED: uncharacterized protein LOC105960... 121 2e-25
gb|AAL75755.1| Putative mutator-like transposase [Oryza sativa J... 121 2e-25
ref|XP_010239711.1| PREDICTED: uncharacterized protein LOC100821... 120 3e-25
ref|XP_012847442.1| PREDICTED: uncharacterized protein LOC105967... 120 4e-25
ref|XP_008664303.1| PREDICTED: uncharacterized protein LOC103642... 120 5e-25
ref|NP_001057710.2| Os06g0502800 [Oryza sativa Japonica Group] g... 120 5e-25
ref|XP_006661020.1| PREDICTED: uncharacterized protein LOC102714... 119 8e-25
>ref|XP_012842534.1| PREDICTED: uncharacterized protein LOC105962758 [Erythranthe
guttatus]
Length = 656
Score = 130 bits (328), Expect = 3e-28
Identities = 57/108 (52%), Positives = 78/108 (72%), Gaps = 1/108 (0%)
Frame = +3
Query: 6 YSGEVLMTNPGSTVIIAM-HEGVFHGIYLCFNACKLGFVNGCRKMIGLDECHLRGNHGGQ 182
Y E+ TNPGSTVI+ + E FH +Y+CFNACK+GF NGCR +IG+D C L+G HGGQ
Sbjct: 379 YCLEIERTNPGSTVIMKLTEERRFHRLYMCFNACKVGFKNGCRPIIGVDGCFLKGGHGGQ 438
Query: 183 ILSAIAMDANDCIYPVAYAVVQKENTASWRWFIMNLGEDLGIENTSDW 326
+L+A+ +D N+ I+P+AYA+V+ E SW WF+ L D+G EN +W
Sbjct: 439 LLTAVGLDPNNNIFPIAYAIVESETKDSWIWFLNLLNADIGFENEHNW 486
>ref|XP_012851396.1| PREDICTED: uncharacterized protein LOC105971096 [Erythranthe
guttatus]
Length = 754
Score = 129 bits (324), Expect = 8e-28
Identities = 56/108 (51%), Positives = 77/108 (71%), Gaps = 1/108 (0%)
Frame = +3
Query: 6 YSGEVLMTNPGSTVIIAM-HEGVFHGIYLCFNACKLGFVNGCRKMIGLDECHLRGNHGGQ 182
Y E+ TNPGSTVI+ + E FH +Y+CFNACK+GF NGCR +IG+D C L+G HGGQ
Sbjct: 184 YCLEIERTNPGSTVIMKLTEERRFHRLYMCFNACKVGFKNGCRPIIGVDGCFLKGGHGGQ 243
Query: 183 ILSAIAMDANDCIYPVAYAVVQKENTASWRWFIMNLGEDLGIENTSDW 326
+L+ + +D N+ I+P+AYA+V+ E SW WF+ L D+G EN +W
Sbjct: 244 LLTVVGLDPNNNIFPIAYAIVESETKDSWIWFLNLLNADIGFENEHNW 291
>emb|CAD40681.2| OSJNBb0118P14.3 [Oryza sativa Japonica Group]
Length = 939
Score = 128 bits (322), Expect = 1e-27
Identities = 56/107 (52%), Positives = 76/107 (71%)
Frame = +3
Query: 6 YSGEVLMTNPGSTVIIAMHEGVFHGIYLCFNACKLGFVNGCRKMIGLDECHLRGNHGGQI 185
Y E+ +NPGS+ + +H G FH +Y+ F+ACK GF+ GCR +I LD CH++ GG I
Sbjct: 403 YGQELRTSNPGSSFYLNLHFGCFHTLYMSFDACKRGFMFGCRPIICLDGCHIKTKFGGHI 462
Query: 186 LSAIAMDANDCIYPVAYAVVQKENTASWRWFIMNLGEDLGIENTSDW 326
L+A+ MD NDCI+P+A AVV+ E+ SW WF+ L +DLGIENTS W
Sbjct: 463 LTAVGMDPNDCIFPIAIAVVEVESLKSWSWFLDTLKKDLGIENTSAW 509
>ref|NP_001050777.2| Os03g0648200 [Oryza sativa Japonica Group]
gi|255674744|dbj|BAF12691.2| Os03g0648200 [Oryza sativa
Japonica Group]
Length = 1015
Score = 127 bits (318), Expect = 4e-27
Identities = 53/107 (49%), Positives = 76/107 (71%)
Frame = +3
Query: 6 YSGEVLMTNPGSTVIIAMHEGVFHGIYLCFNACKLGFVNGCRKMIGLDECHLRGNHGGQI 185
Y E+ +NPGS+ + + +F Y+ +ACK GF+NGCR +I LD CH++ +GGQ+
Sbjct: 453 YGKELRRSNPGSSFFLKLDGSLFSQCYMSMDACKRGFLNGCRPLICLDGCHIKTKYGGQL 512
Query: 186 LSAIAMDANDCIYPVAYAVVQKENTASWRWFIMNLGEDLGIENTSDW 326
L+A+ MD NDCIYP+A+AVV+ E+ A+W+WF+ L DLGIENT W
Sbjct: 513 LTAVGMDPNDCIYPIAFAVVEVESLATWKWFLETLKNDLGIENTYPW 559
>gb|ABF97896.1| transposon protein, putative, Mutator sub-class [Oryza sativa
Japonica Group]
Length = 983
Score = 127 bits (318), Expect = 4e-27
Identities = 53/107 (49%), Positives = 76/107 (71%)
Frame = +3
Query: 6 YSGEVLMTNPGSTVIIAMHEGVFHGIYLCFNACKLGFVNGCRKMIGLDECHLRGNHGGQI 185
Y E+ +NPGS+ + + +F Y+ +ACK GF+NGCR +I LD CH++ +GGQ+
Sbjct: 434 YGKELRRSNPGSSFFLKLDGSLFSQCYMSMDACKRGFLNGCRPLICLDGCHIKTKYGGQL 493
Query: 186 LSAIAMDANDCIYPVAYAVVQKENTASWRWFIMNLGEDLGIENTSDW 326
L+A+ MD NDCIYP+A+AVV+ E+ A+W+WF+ L DLGIENT W
Sbjct: 494 LTAVGMDPNDCIYPIAFAVVEVESLATWKWFLETLKNDLGIENTYPW 540
>gb|AAU89193.1| transposon protein, putative, mutator sub-class [Oryza sativa
Japonica Group]
Length = 1030
Score = 127 bits (318), Expect = 4e-27
Identities = 53/107 (49%), Positives = 76/107 (71%)
Frame = +3
Query: 6 YSGEVLMTNPGSTVIIAMHEGVFHGIYLCFNACKLGFVNGCRKMIGLDECHLRGNHGGQI 185
Y E+ +NPGS+ + + +F Y+ +ACK GF+NGCR +I LD CH++ +GGQ+
Sbjct: 468 YGKELRRSNPGSSFFLKLDGSLFSQCYMSMDACKRGFLNGCRPLICLDGCHIKTKYGGQL 527
Query: 186 LSAIAMDANDCIYPVAYAVVQKENTASWRWFIMNLGEDLGIENTSDW 326
L+A+ MD NDCIYP+A+AVV+ E+ A+W+WF+ L DLGIENT W
Sbjct: 528 LTAVGMDPNDCIYPIAFAVVEVESLATWKWFLETLKNDLGIENTYPW 574
>emb|CAH67772.1| H0322F07.9 [Oryza sativa Indica Group] gi|90399190|emb|CAH68177.1|
H0403D02.5 [Oryza sativa Indica Group]
Length = 1030
Score = 125 bits (315), Expect = 9e-27
Identities = 52/107 (48%), Positives = 76/107 (71%)
Frame = +3
Query: 6 YSGEVLMTNPGSTVIIAMHEGVFHGIYLCFNACKLGFVNGCRKMIGLDECHLRGNHGGQI 185
Y E+ +NPG++ + + +F Y+ +ACK GF+NGCR +I LD CH++ +GGQ+
Sbjct: 468 YGKELRRSNPGTSFFLKLDGSLFSQCYMSMDACKRGFLNGCRPLICLDGCHIKTKYGGQL 527
Query: 186 LSAIAMDANDCIYPVAYAVVQKENTASWRWFIMNLGEDLGIENTSDW 326
L+A+ MD NDCIYP+A+AVV+ E+ A+W+WF+ L DLGIENT W
Sbjct: 528 LTAVGMDPNDCIYPIAFAVVEVESLATWKWFLETLKNDLGIENTYPW 574
>gb|AAT77288.1| putative polyprotein [Oryza sativa Japonica Group]
Length = 1006
Score = 125 bits (315), Expect = 9e-27
Identities = 52/107 (48%), Positives = 76/107 (71%)
Frame = +3
Query: 6 YSGEVLMTNPGSTVIIAMHEGVFHGIYLCFNACKLGFVNGCRKMIGLDECHLRGNHGGQI 185
Y E+ +NPG++ + + +F Y+ +ACK GF+NGCR +I LD CH++ +GGQ+
Sbjct: 444 YGKELRRSNPGTSFFLKLDGSLFSQCYMSMDACKRGFLNGCRPLICLDGCHIKTKYGGQL 503
Query: 186 LSAIAMDANDCIYPVAYAVVQKENTASWRWFIMNLGEDLGIENTSDW 326
L+A+ MD NDCIYP+A+AVV+ E+ A+W+WF+ L DLGIENT W
Sbjct: 504 LTAVGMDPNDCIYPIAFAVVEVESLATWKWFLETLKNDLGIENTYPW 550
>gb|EEC80682.1| hypothetical protein OsI_23105 [Oryza sativa Indica Group]
Length = 926
Score = 125 bits (314), Expect = 1e-26
Identities = 53/108 (49%), Positives = 75/108 (69%)
Frame = +3
Query: 3 SYSGEVLMTNPGSTVIIAMHEGVFHGIYLCFNACKLGFVNGCRKMIGLDECHLRGNHGGQ 182
+Y E+ +NPGS + + + F+ Y+ F+ACK GF++GCR +I LD CH++ GGQ
Sbjct: 264 NYGAELRRSNPGSCFFLNLVDSCFNTCYMSFDACKRGFLSGCRPLICLDGCHIKTKFGGQ 323
Query: 183 ILSAIAMDANDCIYPVAYAVVQKENTASWRWFIMNLGEDLGIENTSDW 326
IL+A+ +D NDCIYP+A VV+ E+ SWRWF+ L EDLGI+NT W
Sbjct: 324 ILTAVGIDPNDCIYPIAIVVVETESLRSWRWFLQTLKEDLGIDNTYPW 371
>gb|AAX93020.1| transposon protein, putative, mutator sub-class [Oryza sativa
Japonica Group] gi|62732961|gb|AAX95080.1| transposon
protein, putative, mutator sub-class [Oryza sativa
Japonica Group] gi|77548842|gb|ABA91639.1| transposon
protein, putative, Mutator sub-class [Oryza sativa
Japonica Group]
Length = 898
Score = 124 bits (312), Expect = 2e-26
Identities = 55/107 (51%), Positives = 75/107 (70%)
Frame = +3
Query: 6 YSGEVLMTNPGSTVIIAMHEGVFHGIYLCFNACKLGFVNGCRKMIGLDECHLRGNHGGQI 185
Y E+ +N GS+ + +H G FH +Y+ F+ACK GF++GCR +I LD CH++ GG I
Sbjct: 422 YGQELRTSNLGSSFYLNLHFGCFHTLYMSFDACKRGFMSGCRPIICLDGCHIKTKFGGHI 481
Query: 186 LSAIAMDANDCIYPVAYAVVQKENTASWRWFIMNLGEDLGIENTSDW 326
L+A+ MD NDCI+P+A AVV E+ SW WF+ L +DLGIENTS W
Sbjct: 482 LTAVGMDPNDCIFPIAIAVVVVESLKSWSWFLDTLKKDLGIENTSAW 528
>ref|XP_011083912.1| PREDICTED: uncharacterized protein LOC105166301 [Sesamum indicum]
Length = 445
Score = 122 bits (305), Expect = 1e-25
Identities = 55/111 (49%), Positives = 77/111 (69%), Gaps = 4/111 (3%)
Frame = +3
Query: 6 YSGEVLMTNPGSTVIIAMH----EGVFHGIYLCFNACKLGFVNGCRKMIGLDECHLRGNH 173
Y+ E+ TNPGSTVI+ E F Y+CF+A K+GF GCRK+IG+D CHL+G H
Sbjct: 254 YADEIRRTNPGSTVIVGTEDVGRENRFSKFYVCFSAMKIGFKEGCRKIIGVDGCHLKGPH 313
Query: 174 GGQILSAIAMDANDCIYPVAYAVVQKENTASWRWFIMNLGEDLGIENTSDW 326
GG +L+A+ +D N+ +YP+AYAVV E+ +W WF++ L DL IEN S++
Sbjct: 314 GGILLTAVGVDPNNNLYPIAYAVVNTESRETWEWFLILLKTDLAIENQSEY 364
>gb|EEC80689.1| hypothetical protein OsI_23113 [Oryza sativa Indica Group]
Length = 539
Score = 121 bits (304), Expect = 2e-25
Identities = 52/108 (48%), Positives = 73/108 (67%)
Frame = +3
Query: 3 SYSGEVLMTNPGSTVIIAMHEGVFHGIYLCFNACKLGFVNGCRKMIGLDECHLRGNHGGQ 182
+Y E+ +NPGS + + + F Y+ F+ACK G ++GCR +I LD CH++ GGQ
Sbjct: 320 NYGAELRRSNPGSCFFLNLVDSCFSTCYISFDACKRGSLSGCRPLICLDGCHIKTKFGGQ 379
Query: 183 ILSAIAMDANDCIYPVAYAVVQKENTASWRWFIMNLGEDLGIENTSDW 326
IL+A+ +D NDCIYP+A VV+ E+ SWRWF+ L EDLGI+NT W
Sbjct: 380 ILTAVGIDPNDCIYPIAIVVVETESLRSWRWFLQTLKEDLGIDNTYPW 427
>gb|AAK63883.1|AC074105_12 Putative transposon protein [Oryza sativa]
gi|31430157|gb|AAP52109.1| transposon protein, putative,
Mutator sub-class [Oryza sativa Japonica Group]
Length = 841
Score = 121 bits (304), Expect = 2e-25
Identities = 52/108 (48%), Positives = 73/108 (67%)
Frame = +3
Query: 3 SYSGEVLMTNPGSTVIIAMHEGVFHGIYLCFNACKLGFVNGCRKMIGLDECHLRGNHGGQ 182
+Y E+ +NPGS + + + F Y+ F+ACK G ++GCR +I LD CH++ GGQ
Sbjct: 313 NYGAELRRSNPGSCFFLNLVDSCFSTCYMSFDACKRGSLSGCRPLICLDGCHIKTKFGGQ 372
Query: 183 ILSAIAMDANDCIYPVAYAVVQKENTASWRWFIMNLGEDLGIENTSDW 326
IL+A+ +D NDCIYP+A VV+ E+ SWRWF+ L EDLGI+NT W
Sbjct: 373 ILTAVGIDPNDCIYPIAIVVVETESLRSWRWFLQTLKEDLGIDNTYPW 420
>ref|XP_012840437.1| PREDICTED: uncharacterized protein LOC105960770 [Erythranthe
guttatus]
Length = 976
Score = 121 bits (303), Expect = 2e-25
Identities = 56/109 (51%), Positives = 75/109 (68%), Gaps = 2/109 (1%)
Frame = +3
Query: 6 YSGEVLMTNPGSTVIIAMH-EGVFHGIYLCFNACKLGFVNGCRKMIGLDECHLRGNHGGQ 182
Y E+ +NPGSTV + ++ E F Y+CFNACK+GF NGCR ++G+D C L+GNHGGQ
Sbjct: 362 YCQELDRSNPGSTVRMQLNDENRFKRFYMCFNACKIGFRNGCRPVVGVDGCWLKGNHGGQ 421
Query: 183 ILSAIAMDANDCIYPVAYAVVQKENTASWRWFIMNLGEDLGI-ENTSDW 326
+LSA+ +D N+ IYP+AYA+V+ E SW WF+ L DL EN W
Sbjct: 422 LLSAVGLDPNNNIYPIAYAIVESETKESWMWFLNMLNIDLNFSENEHTW 470
>gb|AAL75755.1| Putative mutator-like transposase [Oryza sativa Japonica Group]
gi|31429799|gb|AAP51801.1| transposon protein, putative,
Mutator sub-class [Oryza sativa Japonica Group]
Length = 812
Score = 121 bits (303), Expect = 2e-25
Identities = 53/107 (49%), Positives = 74/107 (69%)
Frame = +3
Query: 6 YSGEVLMTNPGSTVIIAMHEGVFHGIYLCFNACKLGFVNGCRKMIGLDECHLRGNHGGQI 185
Y E+ +NP S+ + + G FH +Y+ F+ACK GF++GCR +I LD CH++ GG I
Sbjct: 405 YGQELRTSNPRSSFYLNLLFGCFHTLYMSFDACKRGFLSGCRPIICLDGCHIKTKFGGHI 464
Query: 186 LSAIAMDANDCIYPVAYAVVQKENTASWRWFIMNLGEDLGIENTSDW 326
L+A+ MD NDCI+P+A VV+ E+ SW WF+ L +DLGIENTS W
Sbjct: 465 LTAVGMDPNDCIFPIAIVVVEVESLKSWSWFLDTLKKDLGIENTSAW 511
>ref|XP_010239711.1| PREDICTED: uncharacterized protein LOC100821374 [Brachypodium
distachyon]
Length = 696
Score = 120 bits (302), Expect = 3e-25
Identities = 55/108 (50%), Positives = 77/108 (71%), Gaps = 1/108 (0%)
Frame = +3
Query: 6 YSGEVLMTNPGSTVIIAM-HEGVFHGIYLCFNACKLGFVNGCRKMIGLDECHLRGNHGGQ 182
Y+ EV +NPGST +++ +EG F Y F+ACK GF+ GC +I L CH++ GGQ
Sbjct: 152 YANEVRRSNPGSTFFLSVDNEGRFSKCYFSFDACKRGFLVGCGPLICLYGCHIKTKFGGQ 211
Query: 183 ILSAIAMDANDCIYPVAYAVVQKENTASWRWFIMNLGEDLGIENTSDW 326
+++A+ MD N+CIYPVA+AVV+ E+T SW WF+ L +DLGI+NTS W
Sbjct: 212 LVTAVGMDPNNCIYPVAFAVVEVEDTTSWTWFLNTLKQDLGIDNTSCW 259
>ref|XP_012847442.1| PREDICTED: uncharacterized protein LOC105967392 [Erythranthe
guttatus]
Length = 570
Score = 120 bits (301), Expect = 4e-25
Identities = 55/109 (50%), Positives = 75/109 (68%), Gaps = 2/109 (1%)
Frame = +3
Query: 6 YSGEVLMTNPGSTVIIAMH-EGVFHGIYLCFNACKLGFVNGCRKMIGLDECHLRGNHGGQ 182
Y E+ +NPGSTV + ++ E F Y+CF+ACK+GF NGCR ++G+D C L+GNHGGQ
Sbjct: 239 YCQELDRSNPGSTVRMQLNDENTFKRFYMCFSACKIGFRNGCRPVVGVDGCWLKGNHGGQ 298
Query: 183 ILSAIAMDANDCIYPVAYAVVQKENTASWRWFIMNLGEDLGI-ENTSDW 326
+LSA+ +D N+ IYP+AYA+V+ E SW WF+ L DL EN W
Sbjct: 299 LLSAVGLDPNNNIYPIAYAIVESETKESWMWFLNMLNIDLNFSENEHTW 347
>ref|XP_008664303.1| PREDICTED: uncharacterized protein LOC103642925 [Zea mays]
Length = 530
Score = 120 bits (300), Expect = 5e-25
Identities = 50/107 (46%), Positives = 76/107 (71%)
Frame = +3
Query: 6 YSGEVLMTNPGSTVIIAMHEGVFHGIYLCFNACKLGFVNGCRKMIGLDECHLRGNHGGQI 185
Y E+L +NPGST + + +G F +Y+ +ACK GF++GCR +I LD CHL+ GG +
Sbjct: 396 YGNEILRSNPGSTFYLKLLDGHFSHLYMSLDACKRGFLSGCRPVIFLDGCHLKTKFGGIL 455
Query: 186 LSAIAMDANDCIYPVAYAVVQKENTASWRWFIMNLGEDLGIENTSDW 326
L+A+ +D NDCI+P++ AVV+ E+ A+W+WF+ L +DL I+NT W
Sbjct: 456 LTAVGVDPNDCIFPISMAVVEVESLATWKWFLETLKKDLNIDNTYPW 502
>ref|NP_001057710.2| Os06g0502800 [Oryza sativa Japonica Group]
gi|255677079|dbj|BAF19624.2| Os06g0502800 [Oryza sativa
Japonica Group]
Length = 658
Score = 120 bits (300), Expect = 5e-25
Identities = 52/108 (48%), Positives = 73/108 (67%)
Frame = +3
Query: 3 SYSGEVLMTNPGSTVIIAMHEGVFHGIYLCFNACKLGFVNGCRKMIGLDECHLRGNHGGQ 182
+Y E+ +N GS + + + F Y+ F+ACK GF++GCR +I LD CH++ GQ
Sbjct: 372 NYGAELRRSNLGSCFFLNLVDSCFSTYYMSFDACKRGFLSGCRPLICLDGCHIKTKFSGQ 431
Query: 183 ILSAIAMDANDCIYPVAYAVVQKENTASWRWFIMNLGEDLGIENTSDW 326
IL+A+ +D NDCIYP+A AVV+ E+ SWRWF+ L EDLGI+NT W
Sbjct: 432 ILTAVGIDPNDCIYPIAIAVVETESLRSWRWFLQTLKEDLGIDNTYPW 479
>ref|XP_006661020.1| PREDICTED: uncharacterized protein LOC102714316 [Oryza brachyantha]
Length = 1023
Score = 119 bits (298), Expect = 8e-25
Identities = 51/107 (47%), Positives = 73/107 (68%)
Frame = +3
Query: 6 YSGEVLMTNPGSTVIIAMHEGVFHGIYLCFNACKLGFVNGCRKMIGLDECHLRGNHGGQI 185
Y E+ +NPGST + + +F Y+ +ACK G ++GCR +I LD CH++ GQ+
Sbjct: 488 YGNEIRKSNPGSTFYLKLKGSLFSQCYMSLDACKRGLLSGCRPIICLDGCHIKTKFVGQL 547
Query: 186 LSAIAMDANDCIYPVAYAVVQKENTASWRWFIMNLGEDLGIENTSDW 326
L+AI MD NDCIYP+A AVV+ E+ +W+WF+ L +DLGI+NTS W
Sbjct: 548 LNAIGMDPNDCIYPIAMAVVEVESLVTWKWFLETLVQDLGIDNTSPW 594