BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Forsythia22_contig00033700
         (1877 letters)

Database: ./nr 
           69,698,275 sequences; 24,982,196,650 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011087724.1| PREDICTED: thiamine biosynthetic bifunctiona...   840   0.0  
ref|XP_012850269.1| PREDICTED: thiamine biosynthetic bifunctiona...   801   0.0  
ref|XP_009602814.1| PREDICTED: thiamine biosynthetic bifunctiona...   799   0.0  
ref|XP_006349357.1| PREDICTED: thiamine biosynthetic bifunctiona...   797   0.0  
ref|XP_010658056.1| PREDICTED: thiamine biosynthetic bifunctiona...   797   0.0  
ref|XP_006349358.1| PREDICTED: thiamine biosynthetic bifunctiona...   796   0.0  
ref|XP_010658058.1| PREDICTED: thiamine biosynthetic bifunctiona...   795   0.0  
ref|XP_004230476.2| PREDICTED: thiamine biosynthetic bifunctiona...   794   0.0  
ref|XP_009766916.1| PREDICTED: thiamine biosynthetic bifunctiona...   793   0.0  
ref|XP_009766917.1| PREDICTED: thiamine biosynthetic bifunctiona...   792   0.0  
emb|CBI29762.3| unnamed protein product [Vitis vinifera]              792   0.0  
ref|XP_007042262.1| Thiamin biosynthesis protein isoform 1 [Theo...   788   0.0  
ref|XP_010314660.1| PREDICTED: thiamine biosynthetic bifunctiona...   783   0.0  
emb|CDO97574.1| unnamed protein product [Coffea canephora]            782   0.0  
gb|KHG21691.1| Thiamine biosynthesis bifunctional ThiED [Gossypi...   778   0.0  
ref|XP_008218555.1| PREDICTED: thiamine biosynthetic bifunctiona...   773   0.0  
ref|XP_008218554.1| PREDICTED: thiamine biosynthetic bifunctiona...   773   0.0  
ref|XP_008218553.1| PREDICTED: thiamine biosynthetic bifunctiona...   773   0.0  
ref|XP_012071303.1| PREDICTED: thiamine biosynthetic bifunctiona...   771   0.0  
ref|XP_010101153.1| Thiamine biosynthesis bifunctional protein T...   770   0.0  

>ref|XP_011087724.1| PREDICTED: thiamine biosynthetic bifunctional enzyme TH1,
            chloroplastic [Sesamum indicum]
          Length = 544

 Score =  840 bits (2169), Expect = 0.0
 Identities = 426/531 (80%), Positives = 468/531 (88%)
 Frame = -2

Query: 1750 NHHLRHSPLASSTFHNARVLNLKAVQQEENSRPLVTMSQVRIPHVLTVAGSDSGAGAGIQ 1571
            +H  R+    S     A V NL+A+QQEE  +P     +VR+PHVLTVAGSDSGAGAGIQ
Sbjct: 16   HHQFRYIHSISRGVRRAGVSNLRAMQQEEELKP---PGRVRVPHVLTVAGSDSGAGAGIQ 72

Query: 1570 ADLKACAARGVYCSTVITAVTAQNTVGVQGVSIVSEDFVGEQLRSVLSDMCPDVVKTGML 1391
            AD+KACAARGVYCSTV+TAVTAQNTVGVQGV+IV+E+FV EQL+SVLSDM PDVVKTGML
Sbjct: 73   ADMKACAARGVYCSTVVTAVTAQNTVGVQGVNIVTEEFVREQLKSVLSDMRPDVVKTGML 132

Query: 1390 PSTGIVKILLQSLREFPVRALVVDPVMVSTSGDVLAGPSILDSFREELLPMADIVTPNLK 1211
            PS GIVK+L QSL+EF V ALVVDPVMVSTSGDVLAG S+L SFREELLP+ADIVTPNL+
Sbjct: 133  PSPGIVKVLCQSLKEFQVGALVVDPVMVSTSGDVLAGSSVLASFREELLPLADIVTPNLR 192

Query: 1210 EASALLGGVPLETVADMRSAAKSIHDIGPRNVLVKGGDLADSSDAVDVLFDGKDFYEFRS 1031
            EASALLGG PLETVADM SAAKSIH+IGPRNVLVKGGDL  SSDAVDVLFDGK+FYEFRS
Sbjct: 193  EASALLGGAPLETVADMHSAAKSIHNIGPRNVLVKGGDLPSSSDAVDVLFDGKEFYEFRS 252

Query: 1030 SRIKTRNTHGTGCTLASSIAAELARGSSLFFAVKTAKRYVETALDYSKDILIGNGRQGPF 851
            +R+ T NTHGTGCTLASSIAAELARGSS+  A+K AK YVE+ALDYSKDILIG G QGPF
Sbjct: 253  TRVNTSNTHGTGCTLASSIAAELARGSSVLSAIKIAKHYVESALDYSKDILIGGGHQGPF 312

Query: 850  DHMMKLKNSVRKFYGHRPFDPNDLLLYAVTDSRMNKKWGRSITDAVKAALEGGATIVQLR 671
            DH+MKLKNS       R F PNDLLLYAVTDSRMNKKWGRS+ DAVK+A+EGGATIVQLR
Sbjct: 313  DHLMKLKNSAPDLCARRLFSPNDLLLYAVTDSRMNKKWGRSMRDAVKSAIEGGATIVQLR 372

Query: 670  EKDADTRDFLEAAKACLEICRQHGVPLLINDRVDIALACDADGVHVGQSDMPARVVRTLL 491
            EKDADTRDFLEAAK C+EICR HGVPLLINDR+DIALACDADGVHVGQSDMPARV RT+L
Sbjct: 373  EKDADTRDFLEAAKTCVEICRSHGVPLLINDRIDIALACDADGVHVGQSDMPARVARTIL 432

Query: 490  GPDKIIGVSCKTPEQAHVAWIEGADYIGCGGVYPTNTKENNLTVGLDGLKNVCLASKLPV 311
            GPDKIIGVSCKTPEQA  AWI+GADYIGCGGVYPTNTKENNLTVGLDGLK VCL SKLPV
Sbjct: 433  GPDKIIGVSCKTPEQAEKAWIDGADYIGCGGVYPTNTKENNLTVGLDGLKTVCLESKLPV 492

Query: 310  VAIGGISISNVQPIMELGVPSLKGVAVVSSLFDKESVLRETQNLRRVLLAN 158
            VAIGG  ISNV+P+MELGVP+LKGVAVVS+LFD+ESV  ETQNL +V++ +
Sbjct: 493  VAIGGFGISNVRPVMELGVPNLKGVAVVSALFDRESVSTETQNLLKVIIGS 543


>ref|XP_012850269.1| PREDICTED: thiamine biosynthetic bifunctional enzyme TH1,
            chloroplastic [Erythranthe guttatus]
            gi|604313185|gb|EYU26516.1| hypothetical protein
            MIMGU_mgv1a004117mg [Erythranthe guttata]
          Length = 543

 Score =  801 bits (2070), Expect = 0.0
 Identities = 402/518 (77%), Positives = 456/518 (88%)
 Frame = -2

Query: 1720 SSTFHNARVLNLKAVQQEENSRPLVTMSQVRIPHVLTVAGSDSGAGAGIQADLKACAARG 1541
            S  FH  +V + +A+QQ+E       +  VR+PHVLTVAGSDSGAGAGIQADLKAC+ARG
Sbjct: 24   SRGFHRIKVSSPRAMQQQEE------VHTVRVPHVLTVAGSDSGAGAGIQADLKACSARG 77

Query: 1540 VYCSTVITAVTAQNTVGVQGVSIVSEDFVGEQLRSVLSDMCPDVVKTGMLPSTGIVKILL 1361
            VYCSTVITAVTAQNTVGVQGV IV+EDFV EQL+SVLSDM  DVVKTGMLPS GI+K+L 
Sbjct: 78   VYCSTVITAVTAQNTVGVQGVDIVTEDFVREQLKSVLSDMRLDVVKTGMLPSPGIIKVLC 137

Query: 1360 QSLREFPVRALVVDPVMVSTSGDVLAGPSILDSFREELLPMADIVTPNLKEASALLGGVP 1181
            QSL+EF VRALVVDPVMVSTSGDVLAGP IL + REEL P+ADIVTPN++EASALLGG+P
Sbjct: 138  QSLKEFQVRALVVDPVMVSTSGDVLAGPLILATLREELFPLADIVTPNVREASALLGGIP 197

Query: 1180 LETVADMRSAAKSIHDIGPRNVLVKGGDLADSSDAVDVLFDGKDFYEFRSSRIKTRNTHG 1001
            +ETVADMRSAAKSIHDIGPRNVLVKGGDL  SSDA+DVLFDGKDFYEFRS+RIKT NTHG
Sbjct: 198  VETVADMRSAAKSIHDIGPRNVLVKGGDLPASSDAIDVLFDGKDFYEFRSARIKTVNTHG 257

Query: 1000 TGCTLASSIAAELARGSSLFFAVKTAKRYVETALDYSKDILIGNGRQGPFDHMMKLKNSV 821
            TGCTLASSIAAELA+GSS+  A+K AKRYVE+ L YSKDI IG G+QGPFDH MKLKN+ 
Sbjct: 258  TGCTLASSIAAELAKGSSVLSAIKIAKRYVESVLGYSKDIRIGEGQQGPFDHSMKLKNNT 317

Query: 820  RKFYGHRPFDPNDLLLYAVTDSRMNKKWGRSITDAVKAALEGGATIVQLREKDADTRDFL 641
                  RPFDP+DLLLYAVTDSRMNKKWGRS++DAVKAA+EGGATI+QLREKD+DT DFL
Sbjct: 318  PNLCARRPFDPSDLLLYAVTDSRMNKKWGRSMSDAVKAAIEGGATIIQLREKDSDTGDFL 377

Query: 640  EAAKACLEICRQHGVPLLINDRVDIALACDADGVHVGQSDMPARVVRTLLGPDKIIGVSC 461
            +AAK C+EICR HG+PLLINDR+D+ALACDADGVH+GQSDMPA+  R +LGP+KIIGVSC
Sbjct: 378  KAAKTCVEICRPHGIPLLINDRIDVALACDADGVHIGQSDMPAQTARAILGPEKIIGVSC 437

Query: 460  KTPEQAHVAWIEGADYIGCGGVYPTNTKENNLTVGLDGLKNVCLASKLPVVAIGGISISN 281
            KTPEQA  AWI GADYIGCGGVYPTNTKENN+TVGL+GL+ VCL SKLPVVAIGGI  SN
Sbjct: 438  KTPEQAEKAWIGGADYIGCGGVYPTNTKENNVTVGLEGLRRVCLESKLPVVAIGGIGSSN 497

Query: 280  VQPIMELGVPSLKGVAVVSSLFDKESVLRETQNLRRVL 167
            V+ +MELGVP+L+GVAVVS+LFD+ESV  ET+NL  ++
Sbjct: 498  VKHVMELGVPNLRGVAVVSALFDRESVSGETRNLMTLI 535


>ref|XP_009602814.1| PREDICTED: thiamine biosynthetic bifunctional enzyme TH1,
            chloroplastic [Nicotiana tomentosiformis]
          Length = 550

 Score =  799 bits (2063), Expect = 0.0
 Identities = 414/549 (75%), Positives = 463/549 (84%), Gaps = 3/549 (0%)
 Frame = -2

Query: 1804 MGYYSLQALFTTSCTLVNNHHLRHSPLAS--STFHNARVLNLKAVQQEENSRPLVTMS-Q 1634
            M Y+  Q L T+  T  +   +  +P  +   +FH  R L++KA+Q ++ S P  +    
Sbjct: 1    MAYFCSQVLSTSFFTPKSQIPVLLNPGVACIKSFHQYRFLHIKAMQGDKVSTPTPSDECM 60

Query: 1633 VRIPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQNTVGVQGVSIVSEDFV 1454
            VR+PHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQNTVGVQGV+IV EDFV
Sbjct: 61   VRVPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQNTVGVQGVNIVPEDFV 120

Query: 1453 GEQLRSVLSDMCPDVVKTGMLPSTGIVKILLQSLREFPVRALVVDPVMVSTSGDVLAGPS 1274
             EQLRSVLSDM PDVVKTGMLPS GIVK L QSL+EFPV+ALVVDPVMVSTSG  LAGPS
Sbjct: 121  AEQLRSVLSDMHPDVVKTGMLPSIGIVKTLCQSLKEFPVQALVVDPVMVSTSGHTLAGPS 180

Query: 1273 ILDSFREELLPMADIVTPNLKEASALLGGVPLETVADMRSAAKSIHDIGPRNVLVKGGDL 1094
            ILD FREELLPMADI TPNLKEASALLGGVPLET+ADMR AAK+IHDIGPRNVLVKGGDL
Sbjct: 181  ILDIFREELLPMADIATPNLKEASALLGGVPLETIADMRYAAKAIHDIGPRNVLVKGGDL 240

Query: 1093 ADSSDAVDVLFDGKDFYEFRSSRIKTRNTHGTGCTLASSIAAELARGSSLFFAVKTAKRY 914
              S DAVDV +DGKDFYEFRSSRI T NTHGTGCTLASS+AAELA+GS +  AVK AKRY
Sbjct: 241  PASLDAVDVFYDGKDFYEFRSSRIMTPNTHGTGCTLASSVAAELAKGSQMLSAVKVAKRY 300

Query: 913  VETALDYSKDILIGNGRQGPFDHMMKLKNSVRKFYGHRPFDPNDLLLYAVTDSRMNKKWG 734
            +E AL YSK+I IG GRQGP DH++KLK++V++    RPFDP DL LYAVTDSRMN++WG
Sbjct: 301  IEAALSYSKNIAIGGGRQGPMDHLLKLKSNVQR----RPFDPCDLFLYAVTDSRMNERWG 356

Query: 733  RSITDAVKAALEGGATIVQLREKDADTRDFLEAAKACLEICRQHGVPLLINDRVDIALAC 554
            RSI DAVKAALEGGA+I+QLREK+ +T DFLEAAKACL+ICR HGVPLLINDR+D+ALA 
Sbjct: 357  RSIVDAVKAALEGGASIIQLREKEVETSDFLEAAKACLKICRVHGVPLLINDRIDVALAS 416

Query: 553  DADGVHVGQSDMPARVVRTLLGPDKIIGVSCKTPEQAHVAWIEGADYIGCGGVYPTNTKE 374
            DADGVH+GQSDMPA V R LLGPDKIIGVSCKTPE A  AWI+GADYIG GGVYPTNTKE
Sbjct: 417  DADGVHIGQSDMPAHVARVLLGPDKIIGVSCKTPEHAQQAWIDGADYIGSGGVYPTNTKE 476

Query: 373  NNLTVGLDGLKNVCLASKLPVVAIGGISISNVQPIMELGVPSLKGVAVVSSLFDKESVLR 194
            NN T+GLDGLK VCL+SKLPVVAIGGI ISN Q +M LGVP+LKGVAVVS+LFD+E VL 
Sbjct: 477  NNKTIGLDGLKTVCLSSKLPVVAIGGIGISNAQAVMGLGVPNLKGVAVVSALFDRECVLT 536

Query: 193  ETQNLRRVL 167
            ET+ L  VL
Sbjct: 537  ETRKLLEVL 545


>ref|XP_006349357.1| PREDICTED: thiamine biosynthetic bifunctional enzyme TH1,
            chloroplastic-like isoform X1 [Solanum tuberosum]
          Length = 552

 Score =  797 bits (2059), Expect = 0.0
 Identities = 412/548 (75%), Positives = 459/548 (83%), Gaps = 2/548 (0%)
 Frame = -2

Query: 1804 MGYYSLQALFTTSCTLVNNHHLRHSPLAS--STFHNARVLNLKAVQQEENSRPLVTMSQV 1631
            M +   Q L T+  T      +  SP A+   + H  R L LKA+Q ++ S P    S+V
Sbjct: 1    MAHLCFQVLSTSFFTPKPQIPVLLSPGAACIKSIHQYRYLYLKAMQDDKFSTPPCDESKV 60

Query: 1630 RIPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQNTVGVQGVSIVSEDFVG 1451
            R+PHVLTVAGSDSG GAGIQAD+KACAARGVYCSTVITAVTAQNTVGVQGV+IV EDFV 
Sbjct: 61   RVPHVLTVAGSDSGGGAGIQADIKACAARGVYCSTVITAVTAQNTVGVQGVNIVPEDFVA 120

Query: 1450 EQLRSVLSDMCPDVVKTGMLPSTGIVKILLQSLREFPVRALVVDPVMVSTSGDVLAGPSI 1271
            EQLRSVLSDM P+VVKTGMLPSTGIVK L QSL+EFPV+ALVVDPVMVSTSG  LAGPSI
Sbjct: 121  EQLRSVLSDMPPNVVKTGMLPSTGIVKTLCQSLKEFPVQALVVDPVMVSTSGHTLAGPSI 180

Query: 1270 LDSFREELLPMADIVTPNLKEASALLGGVPLETVADMRSAAKSIHDIGPRNVLVKGGDLA 1091
            LDSFREELLPMADIVTPNLKEASALLGGVPLET+ADMRSAAK+IHDIGPRNVLVKGGDL 
Sbjct: 181  LDSFREELLPMADIVTPNLKEASALLGGVPLETIADMRSAAKAIHDIGPRNVLVKGGDLP 240

Query: 1090 DSSDAVDVLFDGKDFYEFRSSRIKTRNTHGTGCTLASSIAAELARGSSLFFAVKTAKRYV 911
             S DAVDV +DG DFYEFRSSRI T NTHGTGCTLAS++AAELA+GS +  AVK AKRYV
Sbjct: 241  ASLDAVDVFYDGNDFYEFRSSRIMTPNTHGTGCTLASTVAAELAKGSQMLSAVKVAKRYV 300

Query: 910  ETALDYSKDILIGNGRQGPFDHMMKLKNSVRKFYGHRPFDPNDLLLYAVTDSRMNKKWGR 731
            E  L YSK+I IG G QGP DH++KLK++V +    RPFDP DL LYAVTDSRMNKKWGR
Sbjct: 301  EAVLSYSKNIAIGGGCQGPMDHLLKLKSNVER----RPFDPCDLFLYAVTDSRMNKKWGR 356

Query: 730  SITDAVKAALEGGATIVQLREKDADTRDFLEAAKACLEICRQHGVPLLINDRVDIALACD 551
            SI DAVKAA+EGGA+I+QLREK+ DT DFLEAAKACL+ICR HGVPLLINDR+D+ALA D
Sbjct: 357  SIVDAVKAAIEGGASIIQLREKEVDTGDFLEAAKACLKICRVHGVPLLINDRIDVALASD 416

Query: 550  ADGVHVGQSDMPARVVRTLLGPDKIIGVSCKTPEQAHVAWIEGADYIGCGGVYPTNTKEN 371
            ADGVH+GQSDMPA V R LLGPDKIIGVSCKTPE A  AW++GADYIG GGVYPTNTKEN
Sbjct: 417  ADGVHIGQSDMPAHVARALLGPDKIIGVSCKTPEHAQQAWVDGADYIGSGGVYPTNTKEN 476

Query: 370  NLTVGLDGLKNVCLASKLPVVAIGGISISNVQPIMELGVPSLKGVAVVSSLFDKESVLRE 191
            N T+GLDGLK VC++SKLPVVAIGGI +SN Q +M LGVP+LKGVAVVS+LFD+E V  E
Sbjct: 477  NKTIGLDGLKTVCVSSKLPVVAIGGIGMSNAQAVMRLGVPNLKGVAVVSALFDRECVTTE 536

Query: 190  TQNLRRVL 167
            T+ L  VL
Sbjct: 537  TRKLLEVL 544


>ref|XP_010658056.1| PREDICTED: thiamine biosynthetic bifunctional enzyme TH1,
            chloroplastic isoform X1 [Vitis vinifera]
            gi|731411631|ref|XP_010658057.1| PREDICTED: thiamine
            biosynthetic bifunctional enzyme TH1, chloroplastic
            isoform X1 [Vitis vinifera]
          Length = 565

 Score =  797 bits (2058), Expect = 0.0
 Identities = 401/500 (80%), Positives = 447/500 (89%)
 Frame = -2

Query: 1678 VQQEENSRPLVTMSQVRIPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQN 1499
            V QE+    +   S+++IPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQN
Sbjct: 54   VMQEDRPSTVKDDSKMKIPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQN 113

Query: 1498 TVGVQGVSIVSEDFVGEQLRSVLSDMCPDVVKTGMLPSTGIVKILLQSLREFPVRALVVD 1319
            TVGVQGV+IV EDFV EQL+SVLSDM  DVVKTGMLP+ GIVK+L  SL+EFPV+ALVVD
Sbjct: 114  TVGVQGVNIVPEDFVAEQLKSVLSDMHVDVVKTGMLPTIGIVKVLHHSLKEFPVQALVVD 173

Query: 1318 PVMVSTSGDVLAGPSILDSFREELLPMADIVTPNLKEASALLGGVPLETVADMRSAAKSI 1139
            PVMVSTSGDVLAGPSIL +FREELLPMADIVTPNLKEASALLGG+ LETV+DM +AAK I
Sbjct: 174  PVMVSTSGDVLAGPSILAAFREELLPMADIVTPNLKEASALLGGLQLETVSDMCTAAKLI 233

Query: 1138 HDIGPRNVLVKGGDLADSSDAVDVLFDGKDFYEFRSSRIKTRNTHGTGCTLASSIAAELA 959
            HD+GPRNVLVKGGDL  S DAVD+ FDG DFYE RSSRIKTRNTHGTGCTLAS IAAELA
Sbjct: 234  HDMGPRNVLVKGGDLPSSLDAVDIFFDGDDFYELRSSRIKTRNTHGTGCTLASCIAAELA 293

Query: 958  RGSSLFFAVKTAKRYVETALDYSKDILIGNGRQGPFDHMMKLKNSVRKFYGHRPFDPNDL 779
            +GS +  AVK AK Y+ETALDYSKDI IGNG QGPFDH++KLK+++R  +  + F+P +L
Sbjct: 294  KGSQILSAVKAAKHYIETALDYSKDIAIGNGFQGPFDHLLKLKSNIRNSFRKQAFNPANL 353

Query: 778  LLYAVTDSRMNKKWGRSITDAVKAALEGGATIVQLREKDADTRDFLEAAKACLEICRQHG 599
             LYAVTDS MNKKWGRSIT+AVKAA+EGGATIVQLREKDA+TRDFLEAAKAC+EIC  HG
Sbjct: 354  FLYAVTDSGMNKKWGRSITEAVKAAIEGGATIVQLREKDAETRDFLEAAKACVEICHSHG 413

Query: 598  VPLLINDRVDIALACDADGVHVGQSDMPARVVRTLLGPDKIIGVSCKTPEQAHVAWIEGA 419
            VPLLINDR+D+ALACDADGVHVGQSD+PARVVRTLLGP+KIIGVSCKTPEQA  AWI+GA
Sbjct: 414  VPLLINDRIDVALACDADGVHVGQSDIPARVVRTLLGPEKIIGVSCKTPEQAEKAWIDGA 473

Query: 418  DYIGCGGVYPTNTKENNLTVGLDGLKNVCLASKLPVVAIGGISISNVQPIMELGVPSLKG 239
            DYIGCGGVYPTNTK NN+TVGLDGLK VCLASKLPVVAIGGI+ SN + +ME+GVP+LKG
Sbjct: 474  DYIGCGGVYPTNTKANNITVGLDGLKTVCLASKLPVVAIGGINASNARTVMEIGVPNLKG 533

Query: 238  VAVVSSLFDKESVLRETQNL 179
            VAVVS+LFD+E VL ETQ L
Sbjct: 534  VAVVSALFDRECVLTETQKL 553


>ref|XP_006349358.1| PREDICTED: thiamine biosynthetic bifunctional enzyme TH1,
            chloroplastic-like isoform X2 [Solanum tuberosum]
          Length = 546

 Score =  796 bits (2057), Expect = 0.0
 Identities = 404/516 (78%), Positives = 447/516 (86%)
 Frame = -2

Query: 1714 TFHNARVLNLKAVQQEENSRPLVTMSQVRIPHVLTVAGSDSGAGAGIQADLKACAARGVY 1535
            + H  R L LKA+Q ++ S P    S+VR+PHVLTVAGSDSG GAGIQAD+KACAARGVY
Sbjct: 27   SIHQYRYLYLKAMQDDKFSTPPCDESKVRVPHVLTVAGSDSGGGAGIQADIKACAARGVY 86

Query: 1534 CSTVITAVTAQNTVGVQGVSIVSEDFVGEQLRSVLSDMCPDVVKTGMLPSTGIVKILLQS 1355
            CSTVITAVTAQNTVGVQGV+IV EDFV EQLRSVLSDM P+VVKTGMLPSTGIVK L QS
Sbjct: 87   CSTVITAVTAQNTVGVQGVNIVPEDFVAEQLRSVLSDMPPNVVKTGMLPSTGIVKTLCQS 146

Query: 1354 LREFPVRALVVDPVMVSTSGDVLAGPSILDSFREELLPMADIVTPNLKEASALLGGVPLE 1175
            L+EFPV+ALVVDPVMVSTSG  LAGPSILDSFREELLPMADIVTPNLKEASALLGGVPLE
Sbjct: 147  LKEFPVQALVVDPVMVSTSGHTLAGPSILDSFREELLPMADIVTPNLKEASALLGGVPLE 206

Query: 1174 TVADMRSAAKSIHDIGPRNVLVKGGDLADSSDAVDVLFDGKDFYEFRSSRIKTRNTHGTG 995
            T+ADMRSAAK+IHDIGPRNVLVKGGDL  S DAVDV +DG DFYEFRSSRI T NTHGTG
Sbjct: 207  TIADMRSAAKAIHDIGPRNVLVKGGDLPASLDAVDVFYDGNDFYEFRSSRIMTPNTHGTG 266

Query: 994  CTLASSIAAELARGSSLFFAVKTAKRYVETALDYSKDILIGNGRQGPFDHMMKLKNSVRK 815
            CTLAS++AAELA+GS +  AVK AKRYVE  L YSK+I IG G QGP DH++KLK++V +
Sbjct: 267  CTLASTVAAELAKGSQMLSAVKVAKRYVEAVLSYSKNIAIGGGCQGPMDHLLKLKSNVER 326

Query: 814  FYGHRPFDPNDLLLYAVTDSRMNKKWGRSITDAVKAALEGGATIVQLREKDADTRDFLEA 635
                RPFDP DL LYAVTDSRMNKKWGRSI DAVKAA+EGGA+I+QLREK+ DT DFLEA
Sbjct: 327  ----RPFDPCDLFLYAVTDSRMNKKWGRSIVDAVKAAIEGGASIIQLREKEVDTGDFLEA 382

Query: 634  AKACLEICRQHGVPLLINDRVDIALACDADGVHVGQSDMPARVVRTLLGPDKIIGVSCKT 455
            AKACL+ICR HGVPLLINDR+D+ALA DADGVH+GQSDMPA V R LLGPDKIIGVSCKT
Sbjct: 383  AKACLKICRVHGVPLLINDRIDVALASDADGVHIGQSDMPAHVARALLGPDKIIGVSCKT 442

Query: 454  PEQAHVAWIEGADYIGCGGVYPTNTKENNLTVGLDGLKNVCLASKLPVVAIGGISISNVQ 275
            PE A  AW++GADYIG GGVYPTNTKENN T+GLDGLK VC++SKLPVVAIGGI +SN Q
Sbjct: 443  PEHAQQAWVDGADYIGSGGVYPTNTKENNKTIGLDGLKTVCVSSKLPVVAIGGIGMSNAQ 502

Query: 274  PIMELGVPSLKGVAVVSSLFDKESVLRETQNLRRVL 167
             +M LGVP+LKGVAVVS+LFD+E V  ET+ L  VL
Sbjct: 503  AVMRLGVPNLKGVAVVSALFDRECVTTETRKLLEVL 538


>ref|XP_010658058.1| PREDICTED: thiamine biosynthetic bifunctional enzyme TH1,
            chloroplastic isoform X2 [Vitis vinifera]
          Length = 511

 Score =  795 bits (2054), Expect = 0.0
 Identities = 400/498 (80%), Positives = 446/498 (89%)
 Frame = -2

Query: 1672 QEENSRPLVTMSQVRIPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQNTV 1493
            QE+    +   S+++IPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQNTV
Sbjct: 2    QEDRPSTVKDDSKMKIPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQNTV 61

Query: 1492 GVQGVSIVSEDFVGEQLRSVLSDMCPDVVKTGMLPSTGIVKILLQSLREFPVRALVVDPV 1313
            GVQGV+IV EDFV EQL+SVLSDM  DVVKTGMLP+ GIVK+L  SL+EFPV+ALVVDPV
Sbjct: 62   GVQGVNIVPEDFVAEQLKSVLSDMHVDVVKTGMLPTIGIVKVLHHSLKEFPVQALVVDPV 121

Query: 1312 MVSTSGDVLAGPSILDSFREELLPMADIVTPNLKEASALLGGVPLETVADMRSAAKSIHD 1133
            MVSTSGDVLAGPSIL +FREELLPMADIVTPNLKEASALLGG+ LETV+DM +AAK IHD
Sbjct: 122  MVSTSGDVLAGPSILAAFREELLPMADIVTPNLKEASALLGGLQLETVSDMCTAAKLIHD 181

Query: 1132 IGPRNVLVKGGDLADSSDAVDVLFDGKDFYEFRSSRIKTRNTHGTGCTLASSIAAELARG 953
            +GPRNVLVKGGDL  S DAVD+ FDG DFYE RSSRIKTRNTHGTGCTLAS IAAELA+G
Sbjct: 182  MGPRNVLVKGGDLPSSLDAVDIFFDGDDFYELRSSRIKTRNTHGTGCTLASCIAAELAKG 241

Query: 952  SSLFFAVKTAKRYVETALDYSKDILIGNGRQGPFDHMMKLKNSVRKFYGHRPFDPNDLLL 773
            S +  AVK AK Y+ETALDYSKDI IGNG QGPFDH++KLK+++R  +  + F+P +L L
Sbjct: 242  SQILSAVKAAKHYIETALDYSKDIAIGNGFQGPFDHLLKLKSNIRNSFRKQAFNPANLFL 301

Query: 772  YAVTDSRMNKKWGRSITDAVKAALEGGATIVQLREKDADTRDFLEAAKACLEICRQHGVP 593
            YAVTDS MNKKWGRSIT+AVKAA+EGGATIVQLREKDA+TRDFLEAAKAC+EIC  HGVP
Sbjct: 302  YAVTDSGMNKKWGRSITEAVKAAIEGGATIVQLREKDAETRDFLEAAKACVEICHSHGVP 361

Query: 592  LLINDRVDIALACDADGVHVGQSDMPARVVRTLLGPDKIIGVSCKTPEQAHVAWIEGADY 413
            LLINDR+D+ALACDADGVHVGQSD+PARVVRTLLGP+KIIGVSCKTPEQA  AWI+GADY
Sbjct: 362  LLINDRIDVALACDADGVHVGQSDIPARVVRTLLGPEKIIGVSCKTPEQAEKAWIDGADY 421

Query: 412  IGCGGVYPTNTKENNLTVGLDGLKNVCLASKLPVVAIGGISISNVQPIMELGVPSLKGVA 233
            IGCGGVYPTNTK NN+TVGLDGLK VCLASKLPVVAIGGI+ SN + +ME+GVP+LKGVA
Sbjct: 422  IGCGGVYPTNTKANNITVGLDGLKTVCLASKLPVVAIGGINASNARTVMEIGVPNLKGVA 481

Query: 232  VVSSLFDKESVLRETQNL 179
            VVS+LFD+E VL ETQ L
Sbjct: 482  VVSALFDRECVLTETQKL 499


>ref|XP_004230476.2| PREDICTED: thiamine biosynthetic bifunctional enzyme TH1,
            chloroplastic isoform X2 [Solanum lycopersicum]
          Length = 553

 Score =  794 bits (2051), Expect = 0.0
 Identities = 404/517 (78%), Positives = 452/517 (87%), Gaps = 1/517 (0%)
 Frame = -2

Query: 1714 TFHNARVLNLKAVQQEENSRPL-VTMSQVRIPHVLTVAGSDSGAGAGIQADLKACAARGV 1538
            +FH  R L++KA+Q  + + P     S+VR+PHVLTVAGSDSGAGAGIQAD+KACAARGV
Sbjct: 33   SFHQYRFLHVKAMQDGKFATPTSCDESKVRVPHVLTVAGSDSGAGAGIQADIKACAARGV 92

Query: 1537 YCSTVITAVTAQNTVGVQGVSIVSEDFVGEQLRSVLSDMCPDVVKTGMLPSTGIVKILLQ 1358
            YCSTVITAVTAQNTVGVQGV+IV EDFV EQLRSVLSDM P+VVKTGMLPSTGIVK L Q
Sbjct: 93   YCSTVITAVTAQNTVGVQGVNIVPEDFVAEQLRSVLSDMPPNVVKTGMLPSTGIVKTLCQ 152

Query: 1357 SLREFPVRALVVDPVMVSTSGDVLAGPSILDSFREELLPMADIVTPNLKEASALLGGVPL 1178
            SL+EFPV+ALVVDPVMVSTSG  LAGPSILDSFREELLPMADIVTPNLKEASALLGGVPL
Sbjct: 153  SLKEFPVQALVVDPVMVSTSGHTLAGPSILDSFREELLPMADIVTPNLKEASALLGGVPL 212

Query: 1177 ETVADMRSAAKSIHDIGPRNVLVKGGDLADSSDAVDVLFDGKDFYEFRSSRIKTRNTHGT 998
            ET+ADMRSAAK+IHDIGPRNVLVKGGDL  S DAVDV +DG DFYEFRSSRI T NTHGT
Sbjct: 213  ETIADMRSAAKAIHDIGPRNVLVKGGDLPASLDAVDVFYDGNDFYEFRSSRIMTPNTHGT 272

Query: 997  GCTLASSIAAELARGSSLFFAVKTAKRYVETALDYSKDILIGNGRQGPFDHMMKLKNSVR 818
            GCTLASS+AAELA+GS +  AVK AKRYVE  L YSK+I IG G QGP DH++KLK++V+
Sbjct: 273  GCTLASSVAAELAKGSQMLSAVKVAKRYVEAVLSYSKNIAIGGGCQGPMDHLLKLKSNVQ 332

Query: 817  KFYGHRPFDPNDLLLYAVTDSRMNKKWGRSITDAVKAALEGGATIVQLREKDADTRDFLE 638
            +    R FDP+DL LYAVTDSRMNKKWGRSI DAVKAA+EGGA+I+QLREK+ +T DFLE
Sbjct: 333  R----RRFDPSDLFLYAVTDSRMNKKWGRSIVDAVKAAIEGGASIIQLREKEVETGDFLE 388

Query: 637  AAKACLEICRQHGVPLLINDRVDIALACDADGVHVGQSDMPARVVRTLLGPDKIIGVSCK 458
            AAKACL+ICR HGVPLLINDR+D+ALA DADGVH+GQSDMPA V R LLGPDKIIGVSCK
Sbjct: 389  AAKACLKICRVHGVPLLINDRIDVALASDADGVHIGQSDMPAHVARALLGPDKIIGVSCK 448

Query: 457  TPEQAHVAWIEGADYIGCGGVYPTNTKENNLTVGLDGLKNVCLASKLPVVAIGGISISNV 278
            TPE A  AWI+GADYIG GGVYPTNTKENN T+GLDGLK VC++SKLPVVAIGGI +SN 
Sbjct: 449  TPEHAQQAWIDGADYIGSGGVYPTNTKENNKTIGLDGLKTVCVSSKLPVVAIGGIGMSNA 508

Query: 277  QPIMELGVPSLKGVAVVSSLFDKESVLRETQNLRRVL 167
            Q +M+LGVP+LKGVAVVS+LFD+E V+ ET+ L  VL
Sbjct: 509  QAVMQLGVPNLKGVAVVSALFDRECVMTETRKLLEVL 545


>ref|XP_009766916.1| PREDICTED: thiamine biosynthetic bifunctional enzyme TH1,
            chloroplastic isoform X1 [Nicotiana sylvestris]
          Length = 550

 Score =  793 bits (2049), Expect = 0.0
 Identities = 413/549 (75%), Positives = 460/549 (83%), Gaps = 3/549 (0%)
 Frame = -2

Query: 1804 MGYYSLQALFTTSCTLVNNHHLRHSPLAS--STFHNARVLNLKAVQQEENSRPLVTMS-Q 1634
            M Y+  Q L T+  T      +  +P  +   +FH  R L++KA+Q ++ S P  +    
Sbjct: 1    MAYFCTQVLSTSFFTPKPQIPVLLNPGVACIKSFHQYRFLHIKAMQDDKVSTPTPSDECM 60

Query: 1633 VRIPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQNTVGVQGVSIVSEDFV 1454
            VR+PHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQNTVGVQGV+IV EDFV
Sbjct: 61   VRVPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQNTVGVQGVNIVPEDFV 120

Query: 1453 GEQLRSVLSDMCPDVVKTGMLPSTGIVKILLQSLREFPVRALVVDPVMVSTSGDVLAGPS 1274
             EQLRSVLSDM PDVVKTGMLPS GIVK L QSL+EFPV+ALVVDPVMVSTSG  LAGPS
Sbjct: 121  AEQLRSVLSDMHPDVVKTGMLPSIGIVKTLCQSLKEFPVQALVVDPVMVSTSGHTLAGPS 180

Query: 1273 ILDSFREELLPMADIVTPNLKEASALLGGVPLETVADMRSAAKSIHDIGPRNVLVKGGDL 1094
            ILDSFREELLPMADIVTPNLKEASALLGGVPLET+ADM  AAK+IHDIGPRNVLVKGGDL
Sbjct: 181  ILDSFREELLPMADIVTPNLKEASALLGGVPLETIADMHYAAKAIHDIGPRNVLVKGGDL 240

Query: 1093 ADSSDAVDVLFDGKDFYEFRSSRIKTRNTHGTGCTLASSIAAELARGSSLFFAVKTAKRY 914
              S DAVDV +DGKDFY F+SSRI T NTHGTGCTLASS+AAELA+GS +  AVK AKRY
Sbjct: 241  PASLDAVDVFYDGKDFYVFQSSRIMTPNTHGTGCTLASSVAAELAKGSQMLSAVKVAKRY 300

Query: 913  VETALDYSKDILIGNGRQGPFDHMMKLKNSVRKFYGHRPFDPNDLLLYAVTDSRMNKKWG 734
            +E AL YSK+I IG GRQGP DH++KLK++V++    RPFDP DL LYAVTDSRMNK+WG
Sbjct: 301  IEAALSYSKNIAIGGGRQGPVDHLLKLKSNVQR----RPFDPCDLFLYAVTDSRMNKRWG 356

Query: 733  RSITDAVKAALEGGATIVQLREKDADTRDFLEAAKACLEICRQHGVPLLINDRVDIALAC 554
            RSI DAVKAA+EGGATI+QLREK+ +T DFLEAAKACL+ICR HGVPLLINDR+D+ALA 
Sbjct: 357  RSIVDAVKAAIEGGATIIQLREKEVETSDFLEAAKACLKICRVHGVPLLINDRIDVALAS 416

Query: 553  DADGVHVGQSDMPARVVRTLLGPDKIIGVSCKTPEQAHVAWIEGADYIGCGGVYPTNTKE 374
            DADGVH+GQSDMPA V R LLGPDKIIGVSCKTPE A  AWI+GADYIG GGVYPT TKE
Sbjct: 417  DADGVHIGQSDMPAHVARALLGPDKIIGVSCKTPEHALQAWIDGADYIGSGGVYPTTTKE 476

Query: 373  NNLTVGLDGLKNVCLASKLPVVAIGGISISNVQPIMELGVPSLKGVAVVSSLFDKESVLR 194
            NN T+GLDGLK VCL+SKLPVVAIGGI  SN Q IM LGVP+LKGVAVVS+LFD+E VL 
Sbjct: 477  NNKTIGLDGLKTVCLSSKLPVVAIGGIGTSNAQAIMGLGVPNLKGVAVVSALFDRECVLT 536

Query: 193  ETQNLRRVL 167
            ET+ L  VL
Sbjct: 537  ETRKLLEVL 545


>ref|XP_009766917.1| PREDICTED: thiamine biosynthetic bifunctional enzyme TH1,
            chloroplastic isoform X2 [Nicotiana sylvestris]
          Length = 538

 Score =  792 bits (2046), Expect = 0.0
 Identities = 406/517 (78%), Positives = 448/517 (86%), Gaps = 1/517 (0%)
 Frame = -2

Query: 1714 TFHNARVLNLKAVQQEENSRPLVTMS-QVRIPHVLTVAGSDSGAGAGIQADLKACAARGV 1538
            +FH  R L++KA+Q ++ S P  +    VR+PHVLTVAGSDSGAGAGIQADLKACAARGV
Sbjct: 21   SFHQYRFLHIKAMQDDKVSTPTPSDECMVRVPHVLTVAGSDSGAGAGIQADLKACAARGV 80

Query: 1537 YCSTVITAVTAQNTVGVQGVSIVSEDFVGEQLRSVLSDMCPDVVKTGMLPSTGIVKILLQ 1358
            YCSTVITAVTAQNTVGVQGV+IV EDFV EQLRSVLSDM PDVVKTGMLPS GIVK L Q
Sbjct: 81   YCSTVITAVTAQNTVGVQGVNIVPEDFVAEQLRSVLSDMHPDVVKTGMLPSIGIVKTLCQ 140

Query: 1357 SLREFPVRALVVDPVMVSTSGDVLAGPSILDSFREELLPMADIVTPNLKEASALLGGVPL 1178
            SL+EFPV+ALVVDPVMVSTSG  LAGPSILDSFREELLPMADIVTPNLKEASALLGGVPL
Sbjct: 141  SLKEFPVQALVVDPVMVSTSGHTLAGPSILDSFREELLPMADIVTPNLKEASALLGGVPL 200

Query: 1177 ETVADMRSAAKSIHDIGPRNVLVKGGDLADSSDAVDVLFDGKDFYEFRSSRIKTRNTHGT 998
            ET+ADM  AAK+IHDIGPRNVLVKGGDL  S DAVDV +DGKDFY F+SSRI T NTHGT
Sbjct: 201  ETIADMHYAAKAIHDIGPRNVLVKGGDLPASLDAVDVFYDGKDFYVFQSSRIMTPNTHGT 260

Query: 997  GCTLASSIAAELARGSSLFFAVKTAKRYVETALDYSKDILIGNGRQGPFDHMMKLKNSVR 818
            GCTLASS+AAELA+GS +  AVK AKRY+E AL YSK+I IG GRQGP DH++KLK++V+
Sbjct: 261  GCTLASSVAAELAKGSQMLSAVKVAKRYIEAALSYSKNIAIGGGRQGPVDHLLKLKSNVQ 320

Query: 817  KFYGHRPFDPNDLLLYAVTDSRMNKKWGRSITDAVKAALEGGATIVQLREKDADTRDFLE 638
            +    RPFDP DL LYAVTDSRMNK+WGRSI DAVKAA+EGGATI+QLREK+ +T DFLE
Sbjct: 321  R----RPFDPCDLFLYAVTDSRMNKRWGRSIVDAVKAAIEGGATIIQLREKEVETSDFLE 376

Query: 637  AAKACLEICRQHGVPLLINDRVDIALACDADGVHVGQSDMPARVVRTLLGPDKIIGVSCK 458
            AAKACL+ICR HGVPLLINDR+D+ALA DADGVH+GQSDMPA V R LLGPDKIIGVSCK
Sbjct: 377  AAKACLKICRVHGVPLLINDRIDVALASDADGVHIGQSDMPAHVARALLGPDKIIGVSCK 436

Query: 457  TPEQAHVAWIEGADYIGCGGVYPTNTKENNLTVGLDGLKNVCLASKLPVVAIGGISISNV 278
            TPE A  AWI+GADYIG GGVYPT TKENN T+GLDGLK VCL+SKLPVVAIGGI  SN 
Sbjct: 437  TPEHALQAWIDGADYIGSGGVYPTTTKENNKTIGLDGLKTVCLSSKLPVVAIGGIGTSNA 496

Query: 277  QPIMELGVPSLKGVAVVSSLFDKESVLRETQNLRRVL 167
            Q IM LGVP+LKGVAVVS+LFD+E VL ET+ L  VL
Sbjct: 497  QAIMGLGVPNLKGVAVVSALFDRECVLTETRKLLEVL 533


>emb|CBI29762.3| unnamed protein product [Vitis vinifera]
          Length = 497

 Score =  792 bits (2045), Expect = 0.0
 Identities = 397/485 (81%), Positives = 440/485 (90%)
 Frame = -2

Query: 1633 VRIPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQNTVGVQGVSIVSEDFV 1454
            ++IPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQNTVGVQGV+IV EDFV
Sbjct: 1    MKIPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQNTVGVQGVNIVPEDFV 60

Query: 1453 GEQLRSVLSDMCPDVVKTGMLPSTGIVKILLQSLREFPVRALVVDPVMVSTSGDVLAGPS 1274
             EQL+SVLSDM  DVVKTGMLP+ GIVK+L  SL+EFPV+ALVVDPVMVSTSGDVLAGPS
Sbjct: 61   AEQLKSVLSDMHVDVVKTGMLPTIGIVKVLHHSLKEFPVQALVVDPVMVSTSGDVLAGPS 120

Query: 1273 ILDSFREELLPMADIVTPNLKEASALLGGVPLETVADMRSAAKSIHDIGPRNVLVKGGDL 1094
            IL +FREELLPMADIVTPNLKEASALLGG+ LETV+DM +AAK IHD+GPRNVLVKGGDL
Sbjct: 121  ILAAFREELLPMADIVTPNLKEASALLGGLQLETVSDMCTAAKLIHDMGPRNVLVKGGDL 180

Query: 1093 ADSSDAVDVLFDGKDFYEFRSSRIKTRNTHGTGCTLASSIAAELARGSSLFFAVKTAKRY 914
              S DAVD+ FDG DFYE RSSRIKTRNTHGTGCTLAS IAAELA+GS +  AVK AK Y
Sbjct: 181  PSSLDAVDIFFDGDDFYELRSSRIKTRNTHGTGCTLASCIAAELAKGSQILSAVKAAKHY 240

Query: 913  VETALDYSKDILIGNGRQGPFDHMMKLKNSVRKFYGHRPFDPNDLLLYAVTDSRMNKKWG 734
            +ETALDYSKDI IGNG QGPFDH++KLK+++R  +  + F+P +L LYAVTDS MNKKWG
Sbjct: 241  IETALDYSKDIAIGNGFQGPFDHLLKLKSNIRNSFRKQAFNPANLFLYAVTDSGMNKKWG 300

Query: 733  RSITDAVKAALEGGATIVQLREKDADTRDFLEAAKACLEICRQHGVPLLINDRVDIALAC 554
            RSIT+AVKAA+EGGATIVQLREKDA+TRDFLEAAKAC+EIC  HGVPLLINDR+D+ALAC
Sbjct: 301  RSITEAVKAAIEGGATIVQLREKDAETRDFLEAAKACVEICHSHGVPLLINDRIDVALAC 360

Query: 553  DADGVHVGQSDMPARVVRTLLGPDKIIGVSCKTPEQAHVAWIEGADYIGCGGVYPTNTKE 374
            DADGVHVGQSD+PARVVRTLLGP+KIIGVSCKTPEQA  AWI+GADYIGCGGVYPTNTK 
Sbjct: 361  DADGVHVGQSDIPARVVRTLLGPEKIIGVSCKTPEQAEKAWIDGADYIGCGGVYPTNTKA 420

Query: 373  NNLTVGLDGLKNVCLASKLPVVAIGGISISNVQPIMELGVPSLKGVAVVSSLFDKESVLR 194
            NN+TVGLDGLK VCLASKLPVVAIGGI+ SN + +ME+GVP+LKGVAVVS+LFD+E VL 
Sbjct: 421  NNITVGLDGLKTVCLASKLPVVAIGGINASNARTVMEIGVPNLKGVAVVSALFDRECVLT 480

Query: 193  ETQNL 179
            ETQ L
Sbjct: 481  ETQKL 485


>ref|XP_007042262.1| Thiamin biosynthesis protein isoform 1 [Theobroma cacao]
            gi|508706197|gb|EOX98093.1| Thiamin biosynthesis protein
            isoform 1 [Theobroma cacao]
          Length = 574

 Score =  788 bits (2035), Expect = 0.0
 Identities = 402/536 (75%), Positives = 459/536 (85%), Gaps = 2/536 (0%)
 Frame = -2

Query: 1768 SCTLVNNHHLRHSPLASSTFHNARVLNLKA--VQQEENSRPLVTMSQVRIPHVLTVAGSD 1595
            S     N+ LR S   S        ++L+     QE+ +  L   S+++IPHVLTVAGSD
Sbjct: 27   SAKAYTNNKLRVSNSPSFVLRTKGSIHLQGFVAMQEKGASTLSDDSKIKIPHVLTVAGSD 86

Query: 1594 SGAGAGIQADLKACAARGVYCSTVITAVTAQNTVGVQGVSIVSEDFVGEQLRSVLSDMCP 1415
            SGAGAGIQADLKACAAR VYCSTVIT+VTAQNTVGVQGVS VSEDFV EQL+SVLSDM  
Sbjct: 87   SGAGAGIQADLKACAARRVYCSTVITSVTAQNTVGVQGVSNVSEDFVAEQLKSVLSDMQV 146

Query: 1414 DVVKTGMLPSTGIVKILLQSLREFPVRALVVDPVMVSTSGDVLAGPSILDSFREELLPMA 1235
            DVVKTGMLPS GIVKIL ++LREFPV+ALVVDPVMVSTSGDVLAGPSIL  FREEL PMA
Sbjct: 147  DVVKTGMLPSVGIVKILCKTLREFPVQALVVDPVMVSTSGDVLAGPSILSVFREELFPMA 206

Query: 1234 DIVTPNLKEASALLGGVPLETVADMRSAAKSIHDIGPRNVLVKGGDLADSSDAVDVLFDG 1055
            DI+TPNLKEASALL GV LETV D+RSAA+ ++D+GP+NVLVKGGDL DSSDAVD+LF+G
Sbjct: 207  DIITPNLKEASALLDGVQLETVDDLRSAARLLYDMGPKNVLVKGGDLPDSSDAVDILFNG 266

Query: 1054 KDFYEFRSSRIKTRNTHGTGCTLASSIAAELARGSSLFFAVKTAKRYVETALDYSKDILI 875
             +FYE RS RIKTRNTHGTGC+LAS IAAELA+G  +  AVK AKR+VETALDYSKDI+I
Sbjct: 267  DNFYELRSPRIKTRNTHGTGCSLASCIAAELAKGYLMVSAVKVAKRFVETALDYSKDIVI 326

Query: 874  GNGRQGPFDHMMKLKNSVRKFYGHRPFDPNDLLLYAVTDSRMNKKWGRSITDAVKAALEG 695
            GNG QGPFDH+++LK   +  +G + F+P+DLLLYAVTDS MNKKWGRSITDAVKAA+EG
Sbjct: 327  GNGLQGPFDHLLRLKGHSQDCHGLQAFNPSDLLLYAVTDSEMNKKWGRSITDAVKAAIEG 386

Query: 694  GATIVQLREKDADTRDFLEAAKACLEICRQHGVPLLINDRVDIALACDADGVHVGQSDMP 515
            GATIVQLREKDA+T+DFLE+AKACL+IC  HGVPLLINDR+DIALACDADGVHVGQSDMP
Sbjct: 387  GATIVQLREKDAETQDFLESAKACLKICHSHGVPLLINDRIDIALACDADGVHVGQSDMP 446

Query: 514  ARVVRTLLGPDKIIGVSCKTPEQAHVAWIEGADYIGCGGVYPTNTKENNLTVGLDGLKNV 335
            A V RTLLGP+KIIGVSCKTPEQA  AW++GADYIGCGGVYPTNTKENN+TVGLDGLK V
Sbjct: 447  AHVARTLLGPEKIIGVSCKTPEQAQQAWVDGADYIGCGGVYPTNTKENNITVGLDGLKTV 506

Query: 334  CLASKLPVVAIGGISISNVQPIMELGVPSLKGVAVVSSLFDKESVLRETQNLRRVL 167
            C+ASKLPVVAIGGI +SN + +ME+GVP+LKGVAVVS+LFD+E VL ET+ L  +L
Sbjct: 507  CMASKLPVVAIGGIGVSNARIVMEIGVPNLKGVAVVSALFDRECVLTETRKLHAML 562


>ref|XP_010314660.1| PREDICTED: thiamine biosynthetic bifunctional enzyme TH1,
            chloroplastic isoform X1 [Solanum lycopersicum]
          Length = 554

 Score =  783 bits (2022), Expect = 0.0
 Identities = 401/518 (77%), Positives = 449/518 (86%), Gaps = 2/518 (0%)
 Frame = -2

Query: 1714 TFHNARVLNLKAVQQEENSRPL-VTMSQVRIPHVLTVAGSDSGAGAGIQADLKACAARGV 1538
            +FH  R L++KA+Q  + + P     S+VR+PHVLTVAGSDSGAGAGIQAD+KACAARGV
Sbjct: 33   SFHQYRFLHVKAMQDGKFATPTSCDESKVRVPHVLTVAGSDSGAGAGIQADIKACAARGV 92

Query: 1537 YCSTVITAVTAQNTVGVQGVSIVSEDFVGEQLRSVLSDMCPDVVKTG-MLPSTGIVKILL 1361
            YCSTVITAVTAQNTVGVQGV+IV EDFV EQLRSVLSDM P+VV    MLPSTGIVK L 
Sbjct: 93   YCSTVITAVTAQNTVGVQGVNIVPEDFVAEQLRSVLSDMPPNVVSISCMLPSTGIVKTLC 152

Query: 1360 QSLREFPVRALVVDPVMVSTSGDVLAGPSILDSFREELLPMADIVTPNLKEASALLGGVP 1181
            QSL+EFPV+ALVVDPVMVSTSG  LAGPSILDSFREELLPMADIVTPNLKEASALLGGVP
Sbjct: 153  QSLKEFPVQALVVDPVMVSTSGHTLAGPSILDSFREELLPMADIVTPNLKEASALLGGVP 212

Query: 1180 LETVADMRSAAKSIHDIGPRNVLVKGGDLADSSDAVDVLFDGKDFYEFRSSRIKTRNTHG 1001
            LET+ADMRSAAK+IHDIGPRNVLVKGGDL  S DAVDV +DG DFYEFRSSRI T NTHG
Sbjct: 213  LETIADMRSAAKAIHDIGPRNVLVKGGDLPASLDAVDVFYDGNDFYEFRSSRIMTPNTHG 272

Query: 1000 TGCTLASSIAAELARGSSLFFAVKTAKRYVETALDYSKDILIGNGRQGPFDHMMKLKNSV 821
            TGCTLASS+AAELA+GS +  AVK AKRYVE  L YSK+I IG G QGP DH++KLK++V
Sbjct: 273  TGCTLASSVAAELAKGSQMLSAVKVAKRYVEAVLSYSKNIAIGGGCQGPMDHLLKLKSNV 332

Query: 820  RKFYGHRPFDPNDLLLYAVTDSRMNKKWGRSITDAVKAALEGGATIVQLREKDADTRDFL 641
            ++    R FDP+DL LYAVTDSRMNKKWGRSI DAVKAA+EGGA+I+QLREK+ +T DFL
Sbjct: 333  QR----RRFDPSDLFLYAVTDSRMNKKWGRSIVDAVKAAIEGGASIIQLREKEVETGDFL 388

Query: 640  EAAKACLEICRQHGVPLLINDRVDIALACDADGVHVGQSDMPARVVRTLLGPDKIIGVSC 461
            EAAKACL+ICR HGVPLLINDR+D+ALA DADGVH+GQSDMPA V R LLGPDKIIGVSC
Sbjct: 389  EAAKACLKICRVHGVPLLINDRIDVALASDADGVHIGQSDMPAHVARALLGPDKIIGVSC 448

Query: 460  KTPEQAHVAWIEGADYIGCGGVYPTNTKENNLTVGLDGLKNVCLASKLPVVAIGGISISN 281
            KTPE A  AWI+GADYIG GGVYPTNTKENN T+GLDGLK VC++SKLPVVAIGGI +SN
Sbjct: 449  KTPEHAQQAWIDGADYIGSGGVYPTNTKENNKTIGLDGLKTVCVSSKLPVVAIGGIGMSN 508

Query: 280  VQPIMELGVPSLKGVAVVSSLFDKESVLRETQNLRRVL 167
             Q +M+LGVP+LKGVAVVS+LFD+E V+ ET+ L  VL
Sbjct: 509  AQAVMQLGVPNLKGVAVVSALFDRECVMTETRKLLEVL 546


>emb|CDO97574.1| unnamed protein product [Coffea canephora]
          Length = 546

 Score =  782 bits (2020), Expect = 0.0
 Identities = 400/514 (77%), Positives = 447/514 (86%)
 Frame = -2

Query: 1705 NARVLNLKAVQQEENSRPLVTMSQVRIPHVLTVAGSDSGAGAGIQADLKACAARGVYCST 1526
            + +V  L  V+  E    +    QVRIPHVLTVAGSDSGAGAGIQADLKACAARGVYCST
Sbjct: 26   STKVHQLNKVRGFEVKAAMPPEDQVRIPHVLTVAGSDSGAGAGIQADLKACAARGVYCST 85

Query: 1525 VITAVTAQNTVGVQGVSIVSEDFVGEQLRSVLSDMCPDVVKTGMLPSTGIVKILLQSLRE 1346
            VITAVTAQNTVGVQGV+++ EDFV EQL SVLSDM PDVVKTGMLPSTGIVK+L + L+E
Sbjct: 86   VITAVTAQNTVGVQGVNVLPEDFVLEQLHSVLSDMRPDVVKTGMLPSTGIVKVLCRYLQE 145

Query: 1345 FPVRALVVDPVMVSTSGDVLAGPSILDSFREELLPMADIVTPNLKEASALLGGVPLETVA 1166
            FPVRALVVDPVMVSTSGD LAGPSIL SFREELLPMA+IVTPNLKEASALLGG+PLE++A
Sbjct: 146  FPVRALVVDPVMVSTSGDTLAGPSILSSFREELLPMAEIVTPNLKEASALLGGMPLESLA 205

Query: 1165 DMRSAAKSIHDIGPRNVLVKGGDLADSSDAVDVLFDGKDFYEFRSSRIKTRNTHGTGCTL 986
            DM SAAK IHD GP+NVLVKGGDL  S DAVDVL+DG++++EFR+SRIKT NTHGTGC+L
Sbjct: 206  DMHSAAKYIHDFGPKNVLVKGGDLPASLDAVDVLYDGENYFEFRTSRIKTPNTHGTGCSL 265

Query: 985  ASSIAAELARGSSLFFAVKTAKRYVETALDYSKDILIGNGRQGPFDHMMKLKNSVRKFYG 806
            ASSIAAELA+GSS+  AVK AKR++E+AL YSK+ILIGNG  GPFD  +KLK        
Sbjct: 266  ASSIAAELAKGSSVISAVKVAKRFIESALGYSKNILIGNGPHGPFDPFLKLKKYAHGSSR 325

Query: 805  HRPFDPNDLLLYAVTDSRMNKKWGRSITDAVKAALEGGATIVQLREKDADTRDFLEAAKA 626
               FDP++L LYAVTDSRMN+KW RSITDAVKAA++GGATIVQLREK+ADTRDFLE+AKA
Sbjct: 326  QSLFDPSNLFLYAVTDSRMNQKWDRSITDAVKAAIQGGATIVQLREKEADTRDFLESAKA 385

Query: 625  CLEICRQHGVPLLINDRVDIALACDADGVHVGQSDMPARVVRTLLGPDKIIGVSCKTPEQ 446
            CLEICRQHGVPLLINDRVDIALACDADGVHVGQSDMPA V R LLGPDKIIGVSCKTPEQ
Sbjct: 386  CLEICRQHGVPLLINDRVDIALACDADGVHVGQSDMPAHVARVLLGPDKIIGVSCKTPEQ 445

Query: 445  AHVAWIEGADYIGCGGVYPTNTKENNLTVGLDGLKNVCLASKLPVVAIGGISISNVQPIM 266
            A  AW++GADYIGCGGVYPTNTKENN T+GLDGLK VCLASKLPVVAIGGI ISN   +M
Sbjct: 446  AQEAWVDGADYIGCGGVYPTNTKENNPTLGLDGLKTVCLASKLPVVAIGGIGISNAHSVM 505

Query: 265  ELGVPSLKGVAVVSSLFDKESVLRETQNLRRVLL 164
            +L  P+LKGVAVVS+LFD+ESVL ET+ L +VL+
Sbjct: 506  KLQAPNLKGVAVVSALFDRESVLIETRKLHKVLV 539


>gb|KHG21691.1| Thiamine biosynthesis bifunctional ThiED [Gossypium arboreum]
          Length = 568

 Score =  778 bits (2008), Expect = 0.0
 Identities = 395/560 (70%), Positives = 465/560 (83%), Gaps = 18/560 (3%)
 Frame = -2

Query: 1783 ALFTTSCTLVNNHHLRHSPL---ASSTFHNARVLNLKAV---------------QQEENS 1658
            A F ++ +L N ++L  +P    A    +N RV NL ++                QE+  
Sbjct: 4    ATFLSTLSLANKNYLLLTPTHACAKGYTNNFRVSNLPSIVVRKEGSVRSQGFVAMQEKGG 63

Query: 1657 RPLVTMSQVRIPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQNTVGVQGV 1478
              L   S+ + PHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITA+TAQNTVGVQG 
Sbjct: 64   STLSDDSRTKTPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITALTAQNTVGVQGA 123

Query: 1477 SIVSEDFVGEQLRSVLSDMCPDVVKTGMLPSTGIVKILLQSLREFPVRALVVDPVMVSTS 1298
            S V EDFV +QL+SVLSDM  DVVKTGMLPS GIVK L ++LRE+PV ALVVDPVMVSTS
Sbjct: 124  SNVPEDFVAQQLKSVLSDMQVDVVKTGMLPSVGIVKTLCRTLREYPVHALVVDPVMVSTS 183

Query: 1297 GDVLAGPSILDSFREELLPMADIVTPNLKEASALLGGVPLETVADMRSAAKSIHDIGPRN 1118
            GDVLAGPSIL +FREELLPMADI+TPNLKEASALL G+ LETV DMRSAA+ +H++GP+N
Sbjct: 184  GDVLAGPSILSTFREELLPMADIITPNLKEASALLNGMQLETVDDMRSAARLLHNMGPKN 243

Query: 1117 VLVKGGDLADSSDAVDVLFDGKDFYEFRSSRIKTRNTHGTGCTLASSIAAELARGSSLFF 938
            VLVKGGDL DSSDAVD+L++G +FYE RS RI+TRNTHGTGC+LASSIAAE+A+G  +  
Sbjct: 244  VLVKGGDLPDSSDAVDILYNGHNFYELRSPRIRTRNTHGTGCSLASSIAAEVAKGYPMLS 303

Query: 937  AVKTAKRYVETALDYSKDILIGNGRQGPFDHMMKLKNSVRKFYGHRPFDPNDLLLYAVTD 758
            AVK AKR+VETALDYSK+I+IGNG QGPFDH+++LK+  +  +  +PFDP+DL LYAVTD
Sbjct: 304  AVKVAKRFVETALDYSKEIVIGNGPQGPFDHLLRLKSHSQDCHRQQPFDPSDLFLYAVTD 363

Query: 757  SRMNKKWGRSITDAVKAALEGGATIVQLREKDADTRDFLEAAKACLEICRQHGVPLLIND 578
            SRMNK+WGRSI DAVKAA++GGATI+QLREKDA+T+DFLE+AKACL ICR HGVPLLIND
Sbjct: 364  SRMNKRWGRSIVDAVKAAIDGGATIIQLREKDAETKDFLESAKACLRICRLHGVPLLIND 423

Query: 577  RVDIALACDADGVHVGQSDMPARVVRTLLGPDKIIGVSCKTPEQAHVAWIEGADYIGCGG 398
            RVD+A+ACDADGVHVGQSDMPA V RTLLGP+KIIGVSCKTPEQA  AW++GADYIGCGG
Sbjct: 424  RVDVAIACDADGVHVGQSDMPACVARTLLGPEKIIGVSCKTPEQAEQAWVDGADYIGCGG 483

Query: 397  VYPTNTKENNLTVGLDGLKNVCLASKLPVVAIGGISISNVQPIMELGVPSLKGVAVVSSL 218
            VYPTNTKENN+TVGL+GLK VC+ASKLPVVAIGGI +SN + +ME+  P+LKGVAVVS+L
Sbjct: 484  VYPTNTKENNITVGLNGLKTVCMASKLPVVAIGGIGVSNARAVMEIRTPNLKGVAVVSAL 543

Query: 217  FDKESVLRETQNLRRVLLAN 158
            FD+E V  ET+ L  VLL +
Sbjct: 544  FDRECVQTETRKLHDVLLGS 563


>ref|XP_008218555.1| PREDICTED: thiamine biosynthetic bifunctional enzyme TH1,
            chloroplastic isoform X3 [Prunus mume]
          Length = 557

 Score =  773 bits (1996), Expect = 0.0
 Identities = 388/505 (76%), Positives = 438/505 (86%), Gaps = 1/505 (0%)
 Frame = -2

Query: 1678 VQQEENSRPLVT-MSQVRIPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQ 1502
            ++ + N  P  +  S + IPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQ
Sbjct: 45   MEMQGNGSPTTSNQSTINIPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQ 104

Query: 1501 NTVGVQGVSIVSEDFVGEQLRSVLSDMCPDVVKTGMLPSTGIVKILLQSLREFPVRALVV 1322
            NTVGVQGV++V EDFV EQ++SVLSDM  DVVKTGMLPS GIVKIL Q LRE+PVRALVV
Sbjct: 105  NTVGVQGVNVVPEDFVAEQMKSVLSDMHVDVVKTGMLPSIGIVKILHQHLREYPVRALVV 164

Query: 1321 DPVMVSTSGDVLAGPSILDSFREELLPMADIVTPNLKEASALLGGVPLETVADMRSAAKS 1142
            DPVMVSTSGDVLAGPS+L  FRE+LLPMADI+TPNLKEASALL GV ++TV+DMRSAAK 
Sbjct: 165  DPVMVSTSGDVLAGPSVLAGFREDLLPMADIITPNLKEASALLDGVKIKTVSDMRSAAKL 224

Query: 1141 IHDIGPRNVLVKGGDLADSSDAVDVLFDGKDFYEFRSSRIKTRNTHGTGCTLASSIAAEL 962
            +HD+G RNVLVKGGDL DS DAVD+ FDG+  YE RSSRIKTRNTHGTGCTLAS IAAEL
Sbjct: 225  LHDMGVRNVLVKGGDLPDSLDAVDIFFDGEHLYELRSSRIKTRNTHGTGCTLASCIAAEL 284

Query: 961  ARGSSLFFAVKTAKRYVETALDYSKDILIGNGRQGPFDHMMKLKNSVRKFYGHRPFDPND 782
            A+G+S+  AVK AK +VETALDYSK+I IGNG QGPFDH+MKLK++         F+P+D
Sbjct: 285  AKGASMLEAVKVAKCFVETALDYSKEIFIGNGPQGPFDHLMKLKSNAHNSGRQVRFNPSD 344

Query: 781  LLLYAVTDSRMNKKWGRSITDAVKAALEGGATIVQLREKDADTRDFLEAAKACLEICRQH 602
            L LYAVTDS MN++WG SI+DAVKAA++GGATIVQLREKD DTRDF+EAAK+CL+ICR H
Sbjct: 345  LFLYAVTDSGMNRRWGHSISDAVKAAVQGGATIVQLREKDIDTRDFVEAAKSCLQICRAH 404

Query: 601  GVPLLINDRVDIALACDADGVHVGQSDMPARVVRTLLGPDKIIGVSCKTPEQAHVAWIEG 422
            GVPLLINDR+D+ALACDADGVH+GQSDMPA   R LLGP+KIIGVSCKTPEQA  AWI G
Sbjct: 405  GVPLLINDRIDVALACDADGVHIGQSDMPAHTARALLGPEKIIGVSCKTPEQAEQAWIAG 464

Query: 421  ADYIGCGGVYPTNTKENNLTVGLDGLKNVCLASKLPVVAIGGISISNVQPIMELGVPSLK 242
            ADYIGCGGVYPTNTK NNLTVGLDGLK VCLASKLPVVAIGGI +SN +P+ME+GVP+LK
Sbjct: 465  ADYIGCGGVYPTNTKANNLTVGLDGLKTVCLASKLPVVAIGGIKVSNARPVMEIGVPNLK 524

Query: 241  GVAVVSSLFDKESVLRETQNLRRVL 167
            GVAVVS++FD E VL ET+ L  VL
Sbjct: 525  GVAVVSAIFDSECVLTETRKLHAVL 549


>ref|XP_008218554.1| PREDICTED: thiamine biosynthetic bifunctional enzyme TH1,
            chloroplastic isoform X2 [Prunus mume]
          Length = 562

 Score =  773 bits (1996), Expect = 0.0
 Identities = 388/505 (76%), Positives = 438/505 (86%), Gaps = 1/505 (0%)
 Frame = -2

Query: 1678 VQQEENSRPLVT-MSQVRIPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQ 1502
            ++ + N  P  +  S + IPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQ
Sbjct: 50   MEMQGNGSPTTSNQSTINIPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQ 109

Query: 1501 NTVGVQGVSIVSEDFVGEQLRSVLSDMCPDVVKTGMLPSTGIVKILLQSLREFPVRALVV 1322
            NTVGVQGV++V EDFV EQ++SVLSDM  DVVKTGMLPS GIVKIL Q LRE+PVRALVV
Sbjct: 110  NTVGVQGVNVVPEDFVAEQMKSVLSDMHVDVVKTGMLPSIGIVKILHQHLREYPVRALVV 169

Query: 1321 DPVMVSTSGDVLAGPSILDSFREELLPMADIVTPNLKEASALLGGVPLETVADMRSAAKS 1142
            DPVMVSTSGDVLAGPS+L  FRE+LLPMADI+TPNLKEASALL GV ++TV+DMRSAAK 
Sbjct: 170  DPVMVSTSGDVLAGPSVLAGFREDLLPMADIITPNLKEASALLDGVKIKTVSDMRSAAKL 229

Query: 1141 IHDIGPRNVLVKGGDLADSSDAVDVLFDGKDFYEFRSSRIKTRNTHGTGCTLASSIAAEL 962
            +HD+G RNVLVKGGDL DS DAVD+ FDG+  YE RSSRIKTRNTHGTGCTLAS IAAEL
Sbjct: 230  LHDMGVRNVLVKGGDLPDSLDAVDIFFDGEHLYELRSSRIKTRNTHGTGCTLASCIAAEL 289

Query: 961  ARGSSLFFAVKTAKRYVETALDYSKDILIGNGRQGPFDHMMKLKNSVRKFYGHRPFDPND 782
            A+G+S+  AVK AK +VETALDYSK+I IGNG QGPFDH+MKLK++         F+P+D
Sbjct: 290  AKGASMLEAVKVAKCFVETALDYSKEIFIGNGPQGPFDHLMKLKSNAHNSGRQVRFNPSD 349

Query: 781  LLLYAVTDSRMNKKWGRSITDAVKAALEGGATIVQLREKDADTRDFLEAAKACLEICRQH 602
            L LYAVTDS MN++WG SI+DAVKAA++GGATIVQLREKD DTRDF+EAAK+CL+ICR H
Sbjct: 350  LFLYAVTDSGMNRRWGHSISDAVKAAVQGGATIVQLREKDIDTRDFVEAAKSCLQICRAH 409

Query: 601  GVPLLINDRVDIALACDADGVHVGQSDMPARVVRTLLGPDKIIGVSCKTPEQAHVAWIEG 422
            GVPLLINDR+D+ALACDADGVH+GQSDMPA   R LLGP+KIIGVSCKTPEQA  AWI G
Sbjct: 410  GVPLLINDRIDVALACDADGVHIGQSDMPAHTARALLGPEKIIGVSCKTPEQAEQAWIAG 469

Query: 421  ADYIGCGGVYPTNTKENNLTVGLDGLKNVCLASKLPVVAIGGISISNVQPIMELGVPSLK 242
            ADYIGCGGVYPTNTK NNLTVGLDGLK VCLASKLPVVAIGGI +SN +P+ME+GVP+LK
Sbjct: 470  ADYIGCGGVYPTNTKANNLTVGLDGLKTVCLASKLPVVAIGGIKVSNARPVMEIGVPNLK 529

Query: 241  GVAVVSSLFDKESVLRETQNLRRVL 167
            GVAVVS++FD E VL ET+ L  VL
Sbjct: 530  GVAVVSAIFDSECVLTETRKLHAVL 554


>ref|XP_008218553.1| PREDICTED: thiamine biosynthetic bifunctional enzyme TH1,
            chloroplastic isoform X1 [Prunus mume]
          Length = 565

 Score =  773 bits (1996), Expect = 0.0
 Identities = 388/505 (76%), Positives = 438/505 (86%), Gaps = 1/505 (0%)
 Frame = -2

Query: 1678 VQQEENSRPLVT-MSQVRIPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQ 1502
            ++ + N  P  +  S + IPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQ
Sbjct: 53   MEMQGNGSPTTSNQSTINIPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQ 112

Query: 1501 NTVGVQGVSIVSEDFVGEQLRSVLSDMCPDVVKTGMLPSTGIVKILLQSLREFPVRALVV 1322
            NTVGVQGV++V EDFV EQ++SVLSDM  DVVKTGMLPS GIVKIL Q LRE+PVRALVV
Sbjct: 113  NTVGVQGVNVVPEDFVAEQMKSVLSDMHVDVVKTGMLPSIGIVKILHQHLREYPVRALVV 172

Query: 1321 DPVMVSTSGDVLAGPSILDSFREELLPMADIVTPNLKEASALLGGVPLETVADMRSAAKS 1142
            DPVMVSTSGDVLAGPS+L  FRE+LLPMADI+TPNLKEASALL GV ++TV+DMRSAAK 
Sbjct: 173  DPVMVSTSGDVLAGPSVLAGFREDLLPMADIITPNLKEASALLDGVKIKTVSDMRSAAKL 232

Query: 1141 IHDIGPRNVLVKGGDLADSSDAVDVLFDGKDFYEFRSSRIKTRNTHGTGCTLASSIAAEL 962
            +HD+G RNVLVKGGDL DS DAVD+ FDG+  YE RSSRIKTRNTHGTGCTLAS IAAEL
Sbjct: 233  LHDMGVRNVLVKGGDLPDSLDAVDIFFDGEHLYELRSSRIKTRNTHGTGCTLASCIAAEL 292

Query: 961  ARGSSLFFAVKTAKRYVETALDYSKDILIGNGRQGPFDHMMKLKNSVRKFYGHRPFDPND 782
            A+G+S+  AVK AK +VETALDYSK+I IGNG QGPFDH+MKLK++         F+P+D
Sbjct: 293  AKGASMLEAVKVAKCFVETALDYSKEIFIGNGPQGPFDHLMKLKSNAHNSGRQVRFNPSD 352

Query: 781  LLLYAVTDSRMNKKWGRSITDAVKAALEGGATIVQLREKDADTRDFLEAAKACLEICRQH 602
            L LYAVTDS MN++WG SI+DAVKAA++GGATIVQLREKD DTRDF+EAAK+CL+ICR H
Sbjct: 353  LFLYAVTDSGMNRRWGHSISDAVKAAVQGGATIVQLREKDIDTRDFVEAAKSCLQICRAH 412

Query: 601  GVPLLINDRVDIALACDADGVHVGQSDMPARVVRTLLGPDKIIGVSCKTPEQAHVAWIEG 422
            GVPLLINDR+D+ALACDADGVH+GQSDMPA   R LLGP+KIIGVSCKTPEQA  AWI G
Sbjct: 413  GVPLLINDRIDVALACDADGVHIGQSDMPAHTARALLGPEKIIGVSCKTPEQAEQAWIAG 472

Query: 421  ADYIGCGGVYPTNTKENNLTVGLDGLKNVCLASKLPVVAIGGISISNVQPIMELGVPSLK 242
            ADYIGCGGVYPTNTK NNLTVGLDGLK VCLASKLPVVAIGGI +SN +P+ME+GVP+LK
Sbjct: 473  ADYIGCGGVYPTNTKANNLTVGLDGLKTVCLASKLPVVAIGGIKVSNARPVMEIGVPNLK 532

Query: 241  GVAVVSSLFDKESVLRETQNLRRVL 167
            GVAVVS++FD E VL ET+ L  VL
Sbjct: 533  GVAVVSAIFDSECVLTETRKLHAVL 557


>ref|XP_012071303.1| PREDICTED: thiamine biosynthetic bifunctional enzyme TH1,
            chloroplastic isoform X1 [Jatropha curcas]
          Length = 568

 Score =  771 bits (1991), Expect = 0.0
 Identities = 404/551 (73%), Positives = 453/551 (82%), Gaps = 13/551 (2%)
 Frame = -2

Query: 1780 LFTTSCTLVNNH----HLRHSPLASSTFHNARVLNLKA--------VQQEENSRPLVT-M 1640
            +F  S ++ N +    H +H  L+S      R LNLK         +  E N  P     
Sbjct: 17   VFPCSSSICNKNFLPLHRKHIVLSS------RFLNLKVYPSQTRTFMAMEGNGAPASNDA 70

Query: 1639 SQVRIPHVLTVAGSDSGAGAGIQADLKACAARGVYCSTVITAVTAQNTVGVQGVSIVSED 1460
            S+ +IPHVLTVAGSDSGAGAGIQADLK CAARGV+CSTVITAVTAQNTVGVQGV+IV ED
Sbjct: 71   SKAKIPHVLTVAGSDSGAGAGIQADLKTCAARGVFCSTVITAVTAQNTVGVQGVNIVPED 130

Query: 1459 FVGEQLRSVLSDMCPDVVKTGMLPSTGIVKILLQSLREFPVRALVVDPVMVSTSGDVLAG 1280
            FV EQL+SVLSDM  DVVKTGMLPS GIV IL QSLREFPVRALVVDPVMVSTSGDVLAG
Sbjct: 131  FVAEQLKSVLSDMQVDVVKTGMLPSIGIVNILRQSLREFPVRALVVDPVMVSTSGDVLAG 190

Query: 1279 PSILDSFREELLPMADIVTPNLKEASALLGGVPLETVADMRSAAKSIHDIGPRNVLVKGG 1100
            PSIL  F+EELLPMADI+TPN+KEASALLGG PL+TVADMRSAAK +H IGPRNVLVKGG
Sbjct: 191  PSILSCFQEELLPMADIITPNIKEASALLGGTPLKTVADMRSAAKLLHAIGPRNVLVKGG 250

Query: 1099 DLADSSDAVDVLFDGKDFYEFRSSRIKTRNTHGTGCTLASSIAAELARGSSLFFAVKTAK 920
            DL DS DAVD+ FDGK+++E R+SRIKTRNTHGTGCTLAS IAAELA+GS +  AVK AK
Sbjct: 251  DLPDSLDAVDIFFDGKNYHELRASRIKTRNTHGTGCTLASCIAAELAKGSPMLSAVKIAK 310

Query: 919  RYVETALDYSKDILIGNGRQGPFDHMMKLKNSVRKFYGHRPFDPNDLLLYAVTDSRMNKK 740
            RYVETAL+YSKDI IGNGRQGPFDH++KLKN +        F+P+DL LYAVTDS+MNKK
Sbjct: 311  RYVETALEYSKDIFIGNGRQGPFDHLLKLKNGIHN-CRQETFNPSDLFLYAVTDSQMNKK 369

Query: 739  WGRSITDAVKAALEGGATIVQLREKDADTRDFLEAAKACLEICRQHGVPLLINDRVDIAL 560
            WGRS+ DAVKAA+ GGATIVQLREKDA+TR FLE AKAC+EIC  HGVPLLINDRVD+AL
Sbjct: 370  WGRSMVDAVKAAIGGGATIVQLREKDAETRSFLETAKACVEICHSHGVPLLINDRVDVAL 429

Query: 559  ACDADGVHVGQSDMPARVVRTLLGPDKIIGVSCKTPEQAHVAWIEGADYIGCGGVYPTNT 380
            AC+ADGVHVGQSDM A V RTLLGP+KIIGVSCKTPEQA  AWI+GADYIGCGGVYPTNT
Sbjct: 430  ACNADGVHVGQSDMSALVARTLLGPEKIIGVSCKTPEQAQQAWIDGADYIGCGGVYPTNT 489

Query: 379  KENNLTVGLDGLKNVCLASKLPVVAIGGISISNVQPIMELGVPSLKGVAVVSSLFDKESV 200
            K NN TVGLDGLK VCLASKLPVVAIGGI+ SN   +ME  V +L+GVAVVS+LFDKE+V
Sbjct: 490  KANNTTVGLDGLKAVCLASKLPVVAIGGINASNAGAVMETNVSNLEGVAVVSALFDKENV 549

Query: 199  LRETQNLRRVL 167
               ++N+  +L
Sbjct: 550  ADASRNMHAIL 560


>ref|XP_010101153.1| Thiamine biosynthesis bifunctional protein ThiED [Morus notabilis]
            gi|587898954|gb|EXB87371.1| Thiamine biosynthesis
            bifunctional protein ThiED [Morus notabilis]
          Length = 558

 Score =  770 bits (1989), Expect = 0.0
 Identities = 393/520 (75%), Positives = 444/520 (85%)
 Frame = -2

Query: 1726 LASSTFHNARVLNLKAVQQEENSRPLVTMSQVRIPHVLTVAGSDSGAGAGIQADLKACAA 1547
            L +S  +  + ++     Q +N       S++++PHVLTVAGSDSGAGAGIQADLKACAA
Sbjct: 32   LVNSRINEDKQIHTSMEMQRDNGSITKDHSRLKVPHVLTVAGSDSGAGAGIQADLKACAA 91

Query: 1546 RGVYCSTVITAVTAQNTVGVQGVSIVSEDFVGEQLRSVLSDMCPDVVKTGMLPSTGIVKI 1367
            RGVYCSTVITAVTAQNTVGVQG +IV EDFV +QLRSVLSDM  DVVKTGMLPSTGIVK+
Sbjct: 92   RGVYCSTVITAVTAQNTVGVQGGNIVPEDFVAKQLRSVLSDMQVDVVKTGMLPSTGIVKV 151

Query: 1366 LLQSLREFPVRALVVDPVMVSTSGDVLAGPSILDSFREELLPMADIVTPNLKEASALLGG 1187
            L QSL EFPVR LVVDPVMVSTSGDVLAGPSIL  FREELLPMADIVTPNLKEASALLG 
Sbjct: 152  LRQSLAEFPVRGLVVDPVMVSTSGDVLAGPSILTVFREELLPMADIVTPNLKEASALLG- 210

Query: 1186 VPLETVADMRSAAKSIHDIGPRNVLVKGGDLADSSDAVDVLFDGKDFYEFRSSRIKTRNT 1007
            + LETV+ MRSAAK +HD+GPRNVLVKGGDL DSSDA+D+ FDG + +E RSSRIKTRN+
Sbjct: 211  LKLETVSHMRSAAKLLHDMGPRNVLVKGGDLPDSSDAIDIFFDGDNIFELRSSRIKTRNS 270

Query: 1006 HGTGCTLASSIAAELARGSSLFFAVKTAKRYVETALDYSKDILIGNGRQGPFDHMMKLKN 827
            HGTGCTLAS IAAELA+GSS+  AVK AK +VE+AL YS+DI+IGNG QGPFDH+++LK 
Sbjct: 271  HGTGCTLASCIAAELAKGSSMLQAVKAAKCFVESALGYSRDIVIGNGPQGPFDHLLRLKR 330

Query: 826  SVRKFYGHRPFDPNDLLLYAVTDSRMNKKWGRSITDAVKAALEGGATIVQLREKDADTRD 647
            ++    G   FDP+DL LYAVTDSRMNK+WGRSI++AVKAA+EGGATI+QLREKD +TRD
Sbjct: 331  NIHHSSGKHGFDPSDLFLYAVTDSRMNKRWGRSISEAVKAAIEGGATIIQLREKDIETRD 390

Query: 646  FLEAAKACLEICRQHGVPLLINDRVDIALACDADGVHVGQSDMPARVVRTLLGPDKIIGV 467
            FLEAAK+CLEICR  GVPLLINDRVD+ALACDADGVHVGQSDMPAR  R+LLGP+KIIGV
Sbjct: 391  FLEAAKSCLEICRSRGVPLLINDRVDVALACDADGVHVGQSDMPARAARSLLGPEKIIGV 450

Query: 466  SCKTPEQAHVAWIEGADYIGCGGVYPTNTKENNLTVGLDGLKNVCLASKLPVVAIGGISI 287
            SCKTPEQA  AWI GA+YIGCGGVYPTNTK  NLT+GLDGLK VCLASKLPVVAIGGIS 
Sbjct: 451  SCKTPEQAEQAWINGANYIGCGGVYPTNTKATNLTIGLDGLKAVCLASKLPVVAIGGISA 510

Query: 286  SNVQPIMELGVPSLKGVAVVSSLFDKESVLRETQNLRRVL 167
            SN   +M + VP+LKGVAVVS+LFD+E VL ET NL  VL
Sbjct: 511  SNAGSVMGINVPNLKGVAVVSTLFDRECVLTETGNLHSVL 550