BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Forsythia22_contig00013198
         (2766 letters)

Database: ./nr 
           69,698,275 sequences; 24,982,196,650 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

emb|CDP14785.1| unnamed protein product [Coffea canephora]            740   0.0  
ref|XP_012847874.1| PREDICTED: LOW QUALITY PROTEIN: two pore cal...   731   0.0  
sp|Q75VR0.1|TPC1B_TOBAC RecName: Full=Two pore calcium channel p...   716   0.0  
ref|XP_009782426.1| PREDICTED: two pore calcium channel protein ...   716   0.0  
sp|Q75VR1.1|TPC1A_TOBAC RecName: Full=Two pore calcium channel p...   714   0.0  
ref|XP_006447306.1| hypothetical protein CICLE_v10014388mg [Citr...   701   0.0  
ref|XP_011037333.1| PREDICTED: two pore calcium channel protein ...   704   0.0  
ref|XP_007043598.1| Two-pore channel 1 [Theobroma cacao] gi|5087...   709   0.0  
ref|XP_004243353.1| PREDICTED: two pore calcium channel protein ...   714   0.0  
ref|XP_006357480.1| PREDICTED: two pore calcium channel protein ...   712   0.0  
ref|XP_006447308.1| hypothetical protein CICLE_v10014388mg [Citr...   687   0.0  
ref|XP_012479695.1| PREDICTED: two pore calcium channel protein ...   688   0.0  
ref|XP_008455316.1| PREDICTED: two pore calcium channel protein ...   682   0.0  
ref|XP_012088278.1| PREDICTED: two pore calcium channel protein ...   687   0.0  
ref|XP_006376490.1| calcium channel 1 family protein [Populus tr...   690   0.0  
ref|XP_004136825.1| PREDICTED: two pore calcium channel protein ...   681   0.0  
ref|XP_007198932.1| hypothetical protein PRUPE_ppa001980mg [Prun...   687   0.0  
ref|XP_004303095.1| PREDICTED: two pore calcium channel protein ...   691   0.0  
ref|XP_010656956.1| PREDICTED: two pore channel 1 isoform X1 [Vi...   675   0.0  
ref|XP_010035755.1| PREDICTED: two pore calcium channel protein ...   690   0.0  

>emb|CDP14785.1| unnamed protein product [Coffea canephora]
          Length = 744

 Score =  740 bits (1911), Expect(2) = 0.0
 Identities = 363/493 (73%), Positives = 403/493 (81%)
 Frame = -2

Query: 1748 FVIFEDTEQGRTLFTSYGTTLYQMFVLFTTSNNPDVWIPAYKSSRWYCLFFILYVLLGVY 1569
            FVIFEDTEQG T+FTSYG TLYQMFVLFTTSNNPDVWIPAYK+SRWYCLFF+L+VLLGVY
Sbjct: 243  FVIFEDTEQGNTVFTSYGMTLYQMFVLFTTSNNPDVWIPAYKASRWYCLFFVLFVLLGVY 302

Query: 1568 FVTNLILAVVYDSFKSELVKQVAEKDRVRMRILKKAFNLIDNYDLGYLNKDQCIRLFEEL 1389
            FVTNLILAVVYDSFK+ELVKQVAEKDR+R+R L KAF L D Y+LG+L+KDQCIRLFEEL
Sbjct: 303  FVTNLILAVVYDSFKNELVKQVAEKDRLRIRTLSKAFTLADTYNLGFLDKDQCIRLFEEL 362

Query: 1388 NKYRTLPKISRXXXXXXXXXXXDSHDFKINLDEFADLVNAIALRFQKEDSLPIFESCPTF 1209
            NKYRTLPKIS+           DSHDFKINL+EFADL NAIALRFQKE+S PIFE CP+ 
Sbjct: 363  NKYRTLPKISKEDFELIFDELDDSHDFKINLNEFADLCNAIALRFQKENSEPIFEKCPSI 422

Query: 1208 YHSPASEKLKDFVRSPKFGYIIVFILILNFVAVVIETTLDIENNSAQEAWQKVEFVFGWL 1029
            YHSP SE+LK+FVRSP FGYI+ FIL+LN VAV+IETTLDIE+NSAQ+AWQKVEFVFGW+
Sbjct: 423  YHSPVSERLKNFVRSPTFGYIVAFILVLNLVAVIIETTLDIEDNSAQQAWQKVEFVFGWI 482

Query: 1028 YVLEMALKVYAYGFENYWRDSQNRFDFIVTWVIVIGETTTFVSPHGLTFLSNGEWXXXXX 849
            YVLEM LK+YAYGFENYWRD QNRFDFIVT VIV+GET TFV+P+ LTFLSNGEW     
Sbjct: 483  YVLEMLLKIYAYGFENYWRDGQNRFDFIVTLVIVVGETATFVAPNRLTFLSNGEWIRYLL 542

Query: 848  XXXXXXXXXXXXRVERYRAFVTTFLTLIPSLMPYLGIIFCVLCIYCSLGVQIFGGIVNAG 669
                         V +YRAFV TFLTLIPSLMPYLG IFCV+CIYCS+GVQ+FGGIVNAG
Sbjct: 543  ILRMLRLIRLLMNVPQYRAFVATFLTLIPSLMPYLGTIFCVMCIYCSIGVQVFGGIVNAG 602

Query: 668  NPNLVGTDLSDDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKDLTGTTWSYVYFVS 489
            NP L  TDL+D++YLLFNFNDYPNGMVTLFNLLVMGNWQ WMQSY DLTGT+W+Y YFVS
Sbjct: 603  NPKLESTDLADNNYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQSYVDLTGTSWAYTYFVS 662

Query: 488  FYLISXXXXXXXXXXXXXXXXXXXXXXETSEKSEDGVGKEERGKDRRRNVGTKSRSQRVD 309
            FYLI+                      ETSEK ED   +E   K+ RRN+G KSR QRVD
Sbjct: 663  FYLITVLLLLNLVVAFVLEAFFAEMELETSEKCEDTETQEGSRKEHRRNIGMKSRGQRVD 722

Query: 308  ILLHHMLSAELDK 270
            +LLHHMLSAELDK
Sbjct: 723  MLLHHMLSAELDK 735



 Score =  276 bits (705), Expect(2) = 0.0
 Identities = 142/238 (59%), Positives = 170/238 (71%), Gaps = 4/238 (1%)
 Frame = -3

Query: 2464 METALLXXXXXXXXXXSPRRDRKHIFNRRSDAIAYGSTYQKAAALVDLAADGIGIPEEIL 2285
            ME ALL          +  R RK  F+RRSDAIAYGSTYQKAAALVDLA DGIG+PEE+L
Sbjct: 1    MEEALLGGESSGTHSSAWGRIRKGGFSRRSDAIAYGSTYQKAAALVDLAEDGIGLPEEVL 60

Query: 2284 DESSFGSSLKSYFVFIQFDFIWSLNYFALIVLNFLEKPLWCSE----NCSNRVYYFLGEL 2117
            DESSF ++ K YF FI+FDF+W+LNYFALI+LNFLEKPLWCS+     CS+R Y+FLG+L
Sbjct: 61   DESSFANAAKYYFTFIKFDFLWTLNYFALIILNFLEKPLWCSKYSVHTCSDREYFFLGQL 120

Query: 2116 PYLTGTESLIYEGITLLVLIIHIFFPITYEGFGIYWKSXXXXXXXXXXXXXXXXXXXXXX 1937
            P+LT TESLIYEG+TLL+L++HI FP++YEG  IYWK                       
Sbjct: 121  PFLTSTESLIYEGVTLLILVVHILFPLSYEGLAIYWKRLLNQLKVLLLLIMVADILVYVL 180

Query: 1936 XLSPVAFYTLPFRIAPYIRVVLFILNIRELRESTVVLAGMLSTYSNVLGLCGFYFFYS 1763
             LSPVAF +LPFRIAPY+RVV FIL+IR+L  S V+LAGML TY NVL L   +  +S
Sbjct: 181  SLSPVAFNSLPFRIAPYVRVVFFILSIRDLCNSIVILAGMLGTYLNVLALSLLFLLFS 238


>ref|XP_012847874.1| PREDICTED: LOW QUALITY PROTEIN: two pore calcium channel protein 1A
            [Erythranthe guttatus]
          Length = 720

 Score =  731 bits (1886), Expect(2) = 0.0
 Identities = 364/491 (74%), Positives = 394/491 (80%)
 Frame = -2

Query: 1748 FVIFEDTEQGRTLFTSYGTTLYQMFVLFTTSNNPDVWIPAYKSSRWYCLFFILYVLLGVY 1569
            +VIFEDTEQGRT+FTSYG TLYQMFVLFTTSNNPDVWIPAYK+SRWY LFF+LYVLLGVY
Sbjct: 231  YVIFEDTEQGRTVFTSYGVTLYQMFVLFTTSNNPDVWIPAYKASRWYSLFFVLYVLLGVY 290

Query: 1568 FVTNLILAVVYDSFKSELVKQVAEKDRVRMRILKKAFNLIDNYDLGYLNKDQCIRLFEEL 1389
            FVTNLILAVVYDSFKSELVKQVA KD  R R+LKKAFNL+DN DLGYLNKDQCI LFEEL
Sbjct: 291  FVTNLILAVVYDSFKSELVKQVAAKDNQRTRVLKKAFNLLDN-DLGYLNKDQCILLFEEL 349

Query: 1388 NKYRTLPKISRXXXXXXXXXXXDSHDFKINLDEFADLVNAIALRFQKEDSLPIFESCPTF 1209
            NKYR+LP+ISR           DSHD KINLDEF DL +AI LRFQKE+S PIFESCP F
Sbjct: 350  NKYRSLPQISRDDFELIFYELDDSHDVKINLDEFYDLSHAIGLRFQKENSEPIFESCPAF 409

Query: 1208 YHSPASEKLKDFVRSPKFGYIIVFILILNFVAVVIETTLDIENNSAQEAWQKVEFVFGWL 1029
            Y+SPAS  LKDF+R PKFGYII FILI+NF AVVIETTLDI+ NS QEAWQKVEFVFGW+
Sbjct: 410  YNSPASVMLKDFIRCPKFGYIIAFILIMNFAAVVIETTLDIQENSGQEAWQKVEFVFGWI 469

Query: 1028 YVLEMALKVYAYGFENYWRDSQNRFDFIVTWVIVIGETTTFVSPHGLTFLSNGEWXXXXX 849
            YVLEMALKVYAYGF NYWRD QNRFDF++TWVIVIGET TF+SP G TFLSNGEW     
Sbjct: 470  YVLEMALKVYAYGFVNYWRDGQNRFDFVITWVIVIGETATFLSPSGQTFLSNGEWIRYLL 529

Query: 848  XXXXXXXXXXXXRVERYRAFVTTFLTLIPSLMPYLGIIFCVLCIYCSLGVQIFGGIVNAG 669
                         V++YRAFV TFLTL+PSLMPYLG IFC++CIYCSLGVQIFGG+V  G
Sbjct: 530  IARMLRLIRLLMHVKQYRAFVATFLTLLPSLMPYLGTIFCIMCIYCSLGVQIFGGLVYDG 589

Query: 668  NPNLVGTDLSDDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKDLTGTTWSYVYFVS 489
            NPNL GTDL D DYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYK+LTGT WSY YF+S
Sbjct: 590  NPNLTGTDLEDSDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKELTGTAWSYAYFIS 649

Query: 488  FYLISXXXXXXXXXXXXXXXXXXXXXXETSEKSEDGVGKEERGKDRRRNVGTKSRSQRVD 309
            FYLI+                      ET    E     EE+G  RRRN+GTKSRSQRVD
Sbjct: 650  FYLITIVLLLNLVVAFVLEAFFAEMDLETDSGEE-----EEKGTGRRRNIGTKSRSQRVD 704

Query: 308  ILLHHMLSAEL 276
            +LLHHMLSAEL
Sbjct: 705  MLLHHMLSAEL 715



 Score =  262 bits (670), Expect(2) = 0.0
 Identities = 130/217 (59%), Positives = 157/217 (72%)
 Frame = -3

Query: 2413 PRRDRKHIFNRRSDAIAYGSTYQKAAALVDLAADGIGIPEEILDESSFGSSLKSYFVFIQ 2234
            P+ D K +F+RR+DAIA+GS YQKAAALVDLA DGIGIPEEILD+SS+GSSLKSY +FI+
Sbjct: 28   PKLDHKPLFDRRTDAIAHGSPYQKAAALVDLAEDGIGIPEEILDQSSYGSSLKSYLIFIR 87

Query: 2233 FDFIWSLNYFALIVLNFLEKPLWCSENCSNRVYYFLGELPYLTGTESLIYEGITLLVLII 2054
            FDF+WSLNYFALI+LNFLEKPLWCSE CSNR YYFLG+LPYLT  ESLI+EG+TLL+L I
Sbjct: 88   FDFLWSLNYFALILLNFLEKPLWCSEVCSNREYYFLGQLPYLTSAESLIFEGVTLLILFI 147

Query: 2053 HIFFPITYEGFGIYWKSXXXXXXXXXXXXXXXXXXXXXXXLSPVAFYTLPFRIAPYIRVV 1874
            HI FP+TYEG  IYWKS                           +     F I+PY+RV 
Sbjct: 148  HILFPMTYEGCSIYWKSSANRLKVH------------------TSSXFAVFXISPYLRVA 189

Query: 1873 LFILNIRELRESTVVLAGMLSTYSNVLGLCGFYFFYS 1763
             F+L IR++R+S ++L GML TY NVL L   +  +S
Sbjct: 190  FFVLYIRDIRDSIIILVGMLGTYLNVLALWLLFLLFS 226


>sp|Q75VR0.1|TPC1B_TOBAC RecName: Full=Two pore calcium channel protein 1B; AltName:
            Full=Voltage-dependent calcium channel protein TPC1B;
            Short=NtTPC1B [Nicotiana tabacum]
            gi|46275794|dbj|BAD15100.1| two-pore calcium channel
            [Nicotiana tabacum]
          Length = 735

 Score =  716 bits (1847), Expect(2) = 0.0
 Identities = 354/491 (72%), Positives = 390/491 (79%)
 Frame = -2

Query: 1748 FVIFEDTEQGRTLFTSYGTTLYQMFVLFTTSNNPDVWIPAYKSSRWYCLFFILYVLLGVY 1569
            +V FEDT QG+T FTSYGTTLYQMFVLFTTSNNPDVWIPAYK SRWYCLFF+LYVLLGVY
Sbjct: 238  YVFFEDTRQGKTTFTSYGTTLYQMFVLFTTSNNPDVWIPAYKDSRWYCLFFVLYVLLGVY 297

Query: 1568 FVTNLILAVVYDSFKSELVKQVAEKDRVRMRILKKAFNLIDNYDLGYLNKDQCIRLFEEL 1389
            FVTNLILAVVYDSFKSELVKQVA+KDR+R+R LKKAF+LID  + G LN+ QC  LFEEL
Sbjct: 298  FVTNLILAVVYDSFKSELVKQVADKDRLRLRTLKKAFSLIDEANNGLLNEKQCTLLFEEL 357

Query: 1388 NKYRTLPKISRXXXXXXXXXXXDSHDFKINLDEFADLVNAIALRFQKEDSLPIFESCPTF 1209
            NKYRTLPKIS            D+ DFKINL+EFADL +AI LRFQKEDSLPIFE+CP F
Sbjct: 358  NKYRTLPKISGDDFKSIFNELDDTGDFKINLEEFADLCSAIGLRFQKEDSLPIFEACPNF 417

Query: 1208 YHSPASEKLKDFVRSPKFGYIIVFILILNFVAVVIETTLDIENNSAQEAWQKVEFVFGWL 1029
            YHSPASEKL+ F+R   F YIIVF+L++N VAV+IETTLDI+NNS Q  WQKVEF FGWL
Sbjct: 418  YHSPASEKLRGFIRGATFEYIIVFVLLVNLVAVIIETTLDIQNNSGQTFWQKVEFTFGWL 477

Query: 1028 YVLEMALKVYAYGFENYWRDSQNRFDFIVTWVIVIGETTTFVSPHGLTFLSNGEWXXXXX 849
            YV+EMALKVY YGFENYWRD QNRFDFIVTWVIVIGETTTFV+P  LTFLSNGEW     
Sbjct: 478  YVIEMALKVYTYGFENYWRDGQNRFDFIVTWVIVIGETTTFVAPDDLTFLSNGEWIRYLL 537

Query: 848  XXXXXXXXXXXXRVERYRAFVTTFLTLIPSLMPYLGIIFCVLCIYCSLGVQIFGGIVNAG 669
                         VERYRAFV TFLTLIPSLMPYLG IFC+LC YCSLG+QIFGGIVN G
Sbjct: 538  IARMLRLIRLLMHVERYRAFVATFLTLIPSLMPYLGTIFCILCFYCSLGLQIFGGIVNTG 597

Query: 668  NPNLVGTDLSDDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKDLTGTTWSYVYFVS 489
            NPNL  TDL+ +DYLLFNFNDYPNGMVTLFN+LVMGNWQ WMQSYK+LTGT+W+Y YFVS
Sbjct: 598  NPNLAQTDLAGNDYLLFNFNDYPNGMVTLFNILVMGNWQVWMQSYKELTGTSWTYAYFVS 657

Query: 488  FYLISXXXXXXXXXXXXXXXXXXXXXXETSEKSEDGVGKEERGKDRRRNVGTKSRSQRVD 309
            FYLIS                      E S +  DG  KE + ++RRRNVGTK+RSQRVD
Sbjct: 658  FYLISVLWLLNLIVAFVLEAFQAEMDLEASARCVDGDDKEAK-RERRRNVGTKTRSQRVD 716

Query: 308  ILLHHMLSAEL 276
             LLHHML +EL
Sbjct: 717  FLLHHMLRSEL 727



 Score =  256 bits (653), Expect(2) = 0.0
 Identities = 131/220 (59%), Positives = 158/220 (71%), Gaps = 4/220 (1%)
 Frame = -3

Query: 2410 RRDRKHIFNRRSDAIAYGSTYQKAAALVDLAADGIGIPEEILDESSFGSSLKSYFVFIQF 2231
            RR    IF+RR DAIA+GS YQKAAALVDLA DGIG+PEEIL+ +SF  + + YF+F +F
Sbjct: 17   RRRSGSIFDRR-DAIAHGSAYQKAAALVDLAEDGIGLPEEILEGASFEKAAELYFMFTRF 75

Query: 2230 DFIWSLNYFALIVLNFLEKPLWCS----ENCSNRVYYFLGELPYLTGTESLIYEGITLLV 2063
            DF+WSLNY AL+VLNF EKPLWCS    E+C+NR YY+LGELP+LTG ESLI+EG+TLL+
Sbjct: 76   DFLWSLNYLALVVLNFFEKPLWCSKHLAESCNNRDYYYLGELPFLTGAESLIFEGVTLLL 135

Query: 2062 LIIHIFFPITYEGFGIYWKSXXXXXXXXXXXXXXXXXXXXXXXLSPVAFYTLPFRIAPYI 1883
            LIIHI FPI+YEGF +YW+S                         P  FY LPFRIAPY+
Sbjct: 136  LIIHILFPISYEGFNLYWRSLLNRLKVILLLILVADIVVYILL--PADFYYLPFRIAPYL 193

Query: 1882 RVVLFILNIRELRESTVVLAGMLSTYSNVLGLCGFYFFYS 1763
            RVV FILNIRELR+S  +LAGML TY NV+ L   +  +S
Sbjct: 194  RVVFFILNIRELRDSFFILAGMLGTYLNVVALSALFLLFS 233


>ref|XP_009782426.1| PREDICTED: two pore calcium channel protein 1B [Nicotiana sylvestris]
            gi|698464388|ref|XP_009782427.1| PREDICTED: two pore
            calcium channel protein 1B [Nicotiana sylvestris]
          Length = 735

 Score =  716 bits (1847), Expect(2) = 0.0
 Identities = 354/491 (72%), Positives = 390/491 (79%)
 Frame = -2

Query: 1748 FVIFEDTEQGRTLFTSYGTTLYQMFVLFTTSNNPDVWIPAYKSSRWYCLFFILYVLLGVY 1569
            +V FEDT QG+T FTSYGTTLYQMFVLFTTSNNPDVWIPAYK SRWYCLFF+LYVLLGVY
Sbjct: 238  YVFFEDTRQGKTTFTSYGTTLYQMFVLFTTSNNPDVWIPAYKDSRWYCLFFVLYVLLGVY 297

Query: 1568 FVTNLILAVVYDSFKSELVKQVAEKDRVRMRILKKAFNLIDNYDLGYLNKDQCIRLFEEL 1389
            FVTNLILAVVYDSFKSELVKQVA+KDR+R+R LKKAF+LID  + G LN+ QC  LFEEL
Sbjct: 298  FVTNLILAVVYDSFKSELVKQVADKDRLRLRTLKKAFSLIDEANNGLLNEKQCTLLFEEL 357

Query: 1388 NKYRTLPKISRXXXXXXXXXXXDSHDFKINLDEFADLVNAIALRFQKEDSLPIFESCPTF 1209
            NKYRTLPKIS            D+ DFKINL+EFADL +AI LRFQKEDSLPIFE+CP F
Sbjct: 358  NKYRTLPKISGDDFKSIFNELDDTGDFKINLEEFADLCSAIGLRFQKEDSLPIFEACPNF 417

Query: 1208 YHSPASEKLKDFVRSPKFGYIIVFILILNFVAVVIETTLDIENNSAQEAWQKVEFVFGWL 1029
            YHSPASEKL+ F+R   F YIIVF+L++N VAV+IETTLDI+NNS Q  WQKVEF FGWL
Sbjct: 418  YHSPASEKLRGFIRGATFEYIIVFVLLVNLVAVIIETTLDIQNNSGQTFWQKVEFTFGWL 477

Query: 1028 YVLEMALKVYAYGFENYWRDSQNRFDFIVTWVIVIGETTTFVSPHGLTFLSNGEWXXXXX 849
            YV+EMALKVY YGFENYWRD QNRFDFIVTWVIVIGETTTFV+P  LTFLSNGEW     
Sbjct: 478  YVIEMALKVYTYGFENYWRDGQNRFDFIVTWVIVIGETTTFVAPDDLTFLSNGEWIRYLL 537

Query: 848  XXXXXXXXXXXXRVERYRAFVTTFLTLIPSLMPYLGIIFCVLCIYCSLGVQIFGGIVNAG 669
                         VERYRAFV TFLTLIPSLMPYLG IFC+LC YCSLG+QIFGGIVN G
Sbjct: 538  IARMLRLIRLLMHVERYRAFVATFLTLIPSLMPYLGTIFCILCFYCSLGLQIFGGIVNTG 597

Query: 668  NPNLVGTDLSDDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKDLTGTTWSYVYFVS 489
            NPNL  TDL+ +DYLLFNFNDYPNGMVTLFN+LVMGNWQ WMQSYK+LTGT+W+Y YFVS
Sbjct: 598  NPNLAQTDLAGNDYLLFNFNDYPNGMVTLFNILVMGNWQVWMQSYKELTGTSWTYAYFVS 657

Query: 488  FYLISXXXXXXXXXXXXXXXXXXXXXXETSEKSEDGVGKEERGKDRRRNVGTKSRSQRVD 309
            FYLIS                      E S +  DG  KE + ++RRRNVGTK+RSQRVD
Sbjct: 658  FYLISVLWLLNLIVAFVLEAFQAEMDLEASARCVDGDDKEAK-RERRRNVGTKTRSQRVD 716

Query: 308  ILLHHMLSAEL 276
             LLHHML +EL
Sbjct: 717  FLLHHMLRSEL 727



 Score =  253 bits (647), Expect(2) = 0.0
 Identities = 131/220 (59%), Positives = 159/220 (72%), Gaps = 4/220 (1%)
 Frame = -3

Query: 2410 RRDRKHIFNRRSDAIAYGSTYQKAAALVDLAADGIGIPEEILDESSFGSSLKSYFVFIQF 2231
            RR    IF+RR DAIA+GS YQKAAALVDLA DGIG+PEEIL+ +SF  + + YF+F +F
Sbjct: 17   RRRSGSIFDRR-DAIAHGSAYQKAAALVDLAEDGIGLPEEILEGASFEKAAELYFMFTRF 75

Query: 2230 DFIWSLNYFALIVLNFLEKPLWCS----ENCSNRVYYFLGELPYLTGTESLIYEGITLLV 2063
            DF+WSLNY AL+VLNF EKPLWCS    E+C+NR YY+LGELP+LTG ESLI+EG+TLL+
Sbjct: 76   DFLWSLNYLALVVLNFFEKPLWCSKHLAESCNNRDYYYLGELPFLTGAESLIFEGVTLLL 135

Query: 2062 LIIHIFFPITYEGFGIYWKSXXXXXXXXXXXXXXXXXXXXXXXLSPVAFYTLPFRIAPYI 1883
            LIIHI FPI+YEGF +YW+S                       L+   FY LPFRIAPY+
Sbjct: 136  LIIHILFPISYEGFNLYWRSLLNRLKVILLLILVADIVVYILFLAD--FYYLPFRIAPYL 193

Query: 1882 RVVLFILNIRELRESTVVLAGMLSTYSNVLGLCGFYFFYS 1763
            RVV FILNIRELR+S  +LAGML TY NV+ L   +  +S
Sbjct: 194  RVVFFILNIRELRDSFFILAGMLGTYLNVVALSALFLLFS 233


>sp|Q75VR1.1|TPC1A_TOBAC RecName: Full=Two pore calcium channel protein 1A; AltName:
            Full=Voltage-dependent calcium channel protein TPC1A;
            Short=NtTPC1A [Nicotiana tabacum]
            gi|46275792|dbj|BAD15099.1| two-pore calcium channel
            [Nicotiana tabacum]
          Length = 735

 Score =  714 bits (1844), Expect(2) = 0.0
 Identities = 353/491 (71%), Positives = 387/491 (78%)
 Frame = -2

Query: 1748 FVIFEDTEQGRTLFTSYGTTLYQMFVLFTTSNNPDVWIPAYKSSRWYCLFFILYVLLGVY 1569
            +V FEDT QG+T FTSYGTTLYQMFVLFTTSNNPDVWIPAYK SRWYCLFF+LYVLLGVY
Sbjct: 238  YVFFEDTRQGKTTFTSYGTTLYQMFVLFTTSNNPDVWIPAYKDSRWYCLFFVLYVLLGVY 297

Query: 1568 FVTNLILAVVYDSFKSELVKQVAEKDRVRMRILKKAFNLIDNYDLGYLNKDQCIRLFEEL 1389
            FVTNLILAVVYDSFKSELVKQVA+KDR+R+R LKKAF+LID  + G+LN+ QC  LFEEL
Sbjct: 298  FVTNLILAVVYDSFKSELVKQVADKDRLRLRTLKKAFSLIDEANNGHLNEKQCTLLFEEL 357

Query: 1388 NKYRTLPKISRXXXXXXXXXXXDSHDFKINLDEFADLVNAIALRFQKEDSLPIFESCPTF 1209
            NKYRTLPKIS            D+ DFKINLDEFADL  AI LRFQKEDSLPIFE+CP F
Sbjct: 358  NKYRTLPKISGDDFKSIFSELDDTGDFKINLDEFADLCTAIGLRFQKEDSLPIFEACPNF 417

Query: 1208 YHSPASEKLKDFVRSPKFGYIIVFILILNFVAVVIETTLDIENNSAQEAWQKVEFVFGWL 1029
            YHSPASEKL+ FVR   F YIIVF+L++N VAV+IETTLDI+NNS Q  WQKVEF FGWL
Sbjct: 418  YHSPASEKLRGFVRGATFEYIIVFVLLVNLVAVIIETTLDIQNNSGQTFWQKVEFTFGWL 477

Query: 1028 YVLEMALKVYAYGFENYWRDSQNRFDFIVTWVIVIGETTTFVSPHGLTFLSNGEWXXXXX 849
            YV+EMALKVY YGFENYWRD QNRFDF+VTWVIVIGET TFV+P GLTFLSNGEW     
Sbjct: 478  YVIEMALKVYTYGFENYWRDGQNRFDFVVTWVIVIGETATFVAPDGLTFLSNGEWIRYLL 537

Query: 848  XXXXXXXXXXXXRVERYRAFVTTFLTLIPSLMPYLGIIFCVLCIYCSLGVQIFGGIVNAG 669
                         VERYRAFV TF TLIPSL+PYLG IFC+LC YCSLG+QIFGGIVN G
Sbjct: 538  IARMLRLIRLLMHVERYRAFVATFFTLIPSLVPYLGTIFCILCFYCSLGLQIFGGIVNTG 597

Query: 668  NPNLVGTDLSDDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKDLTGTTWSYVYFVS 489
            NPNL  TDL+ +DYLLFNFNDYPNGMVTLFN+LVMGNWQ WMQSYK+LTGT W+Y YFVS
Sbjct: 598  NPNLAQTDLAGNDYLLFNFNDYPNGMVTLFNILVMGNWQVWMQSYKELTGTAWTYAYFVS 657

Query: 488  FYLISXXXXXXXXXXXXXXXXXXXXXXETSEKSEDGVGKEERGKDRRRNVGTKSRSQRVD 309
            FYLIS                      E S +  DG  KE +  +RRRNVGTK+RSQRVD
Sbjct: 658  FYLISVLWLLNLIVAFVLEAFQAEVDLEASARCVDGDDKEAK-SERRRNVGTKTRSQRVD 716

Query: 308  ILLHHMLSAEL 276
             LLHHML +EL
Sbjct: 717  FLLHHMLRSEL 727



 Score =  253 bits (647), Expect(2) = 0.0
 Identities = 131/220 (59%), Positives = 159/220 (72%), Gaps = 4/220 (1%)
 Frame = -3

Query: 2410 RRDRKHIFNRRSDAIAYGSTYQKAAALVDLAADGIGIPEEILDESSFGSSLKSYFVFIQF 2231
            RR    IF+RR DAIA+GS YQKAAALVDLA DGIG+PEEIL+ +SF  + + YF+F +F
Sbjct: 17   RRRIGSIFDRR-DAIAHGSAYQKAAALVDLAEDGIGLPEEILEGASFEKAAELYFIFTRF 75

Query: 2230 DFIWSLNYFALIVLNFLEKPLWCS----ENCSNRVYYFLGELPYLTGTESLIYEGITLLV 2063
            DF+WSLNY AL+VLNF EKPLWCS    E+C+NR YY+LGELP+LTG ESLI+EG+TLL+
Sbjct: 76   DFLWSLNYLALVVLNFFEKPLWCSKHLAESCNNRDYYYLGELPFLTGAESLIFEGVTLLL 135

Query: 2062 LIIHIFFPITYEGFGIYWKSXXXXXXXXXXXXXXXXXXXXXXXLSPVAFYTLPFRIAPYI 1883
            LIIHI FPI+YEGF +YW+S                       L+   FY LPFRIAPY+
Sbjct: 136  LIIHILFPISYEGFNLYWRSLLNRVKVILLLILVADIVVYILFLAD--FYYLPFRIAPYL 193

Query: 1882 RVVLFILNIRELRESTVVLAGMLSTYSNVLGLCGFYFFYS 1763
            RVV FILNIRELR+S  +LAGML TY NV+ L   +  +S
Sbjct: 194  RVVFFILNIRELRDSFFILAGMLGTYLNVVALSALFLLFS 233


>ref|XP_006447306.1| hypothetical protein CICLE_v10014388mg [Citrus clementina]
            gi|568877173|ref|XP_006491622.1| PREDICTED: two pore
            calcium channel protein 1-like isoform X1 [Citrus
            sinensis] gi|557549917|gb|ESR60546.1| hypothetical
            protein CICLE_v10014388mg [Citrus clementina]
          Length = 745

 Score =  701 bits (1810), Expect(2) = 0.0
 Identities = 346/493 (70%), Positives = 390/493 (79%)
 Frame = -2

Query: 1748 FVIFEDTEQGRTLFTSYGTTLYQMFVLFTTSNNPDVWIPAYKSSRWYCLFFILYVLLGVY 1569
            +VIFEDT QG  +FTS+GTTLYQMFVLFTTSNNPDVWIPAYK+SRWYCLFF+L+VL+GVY
Sbjct: 250  YVIFEDTVQGNMVFTSFGTTLYQMFVLFTTSNNPDVWIPAYKASRWYCLFFVLFVLIGVY 309

Query: 1568 FVTNLILAVVYDSFKSELVKQVAEKDRVRMRILKKAFNLIDNYDLGYLNKDQCIRLFEEL 1389
            FVTNLILAVVYDSFKS+L KQV+E DR+R R L KAFNLIDNY++G+LNK+QCI+LFEEL
Sbjct: 310  FVTNLILAVVYDSFKSQLAKQVSEMDRMRRRTLGKAFNLIDNYNVGFLNKEQCIKLFEEL 369

Query: 1388 NKYRTLPKISRXXXXXXXXXXXDSHDFKINLDEFADLVNAIALRFQKEDSLPIFESCPTF 1209
            NKYRTLP ISR           D+HDFKINLDEFADL NAIALRFQKED    FE+ P+ 
Sbjct: 370  NKYRTLPNISREEFELIFDELDDTHDFKINLDEFADLCNAIALRFQKEDVPSCFENLPSI 429

Query: 1208 YHSPASEKLKDFVRSPKFGYIIVFILILNFVAVVIETTLDIENNSAQEAWQKVEFVFGWL 1029
            YHSP SEKLK F+RS KFGY+I  ILI+N VAV++ETTLDI+ +S Q  WQ+VEFVFGW+
Sbjct: 430  YHSPFSEKLKAFIRSTKFGYMISIILIVNLVAVIVETTLDIQESSLQSVWQEVEFVFGWI 489

Query: 1028 YVLEMALKVYAYGFENYWRDSQNRFDFIVTWVIVIGETTTFVSPHGLTFLSNGEWXXXXX 849
            YVLEMALK+Y+YGFENYWRD QNRFDF+VTWVIVIGET T  SP+G TFLSNGEW     
Sbjct: 490  YVLEMALKIYSYGFENYWRDGQNRFDFLVTWVIVIGETITLASPNGQTFLSNGEWIRYLL 549

Query: 848  XXXXXXXXXXXXRVERYRAFVTTFLTLIPSLMPYLGIIFCVLCIYCSLGVQIFGGIVNAG 669
                         V++YR FV TFLTLIPSLMPYLG IFCV CIYCSLGVQIFGGIVNAG
Sbjct: 550  LARMLRLIRLLMHVQQYRGFVATFLTLIPSLMPYLGTIFCVQCIYCSLGVQIFGGIVNAG 609

Query: 668  NPNLVGTDLSDDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKDLTGTTWSYVYFVS 489
            N  L  TDL+DDDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQSYK+LTGT W+  YFVS
Sbjct: 610  NAKLEETDLADDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQSYKELTGTAWTLAYFVS 669

Query: 488  FYLISXXXXXXXXXXXXXXXXXXXXXXETSEKSEDGVGKEERGKDRRRNVGTKSRSQRVD 309
            FYLI+                      E+SEK E+   K+   ++RRR VGTK+RSQRVD
Sbjct: 670  FYLITVLLLLNLVIAFVLEAFFAEMELESSEKCEE-EDKDGEPRERRRRVGTKTRSQRVD 728

Query: 308  ILLHHMLSAELDK 270
            +LLHHMLSAEL K
Sbjct: 729  VLLHHMLSAELQK 741



 Score =  265 bits (677), Expect(2) = 0.0
 Identities = 138/222 (62%), Positives = 160/222 (72%), Gaps = 6/222 (2%)
 Frame = -3

Query: 2410 RRDRKHIFNRRSDAIAYGSTYQKAAALVDLAADGIGIPEEILDESSFGSSLKSYFVFIQF 2231
            RR    +F RR+DAI  GS YQKAAALVDLA DGIG+PEEILD SSF SS K YF+FI+F
Sbjct: 24   RRGPTALFRRRTDAITRGSPYQKAAALVDLAEDGIGLPEEILDRSSFESSAKFYFIFIKF 83

Query: 2230 DFIWSLNYFALIVLNFLEKPLWCSE------NCSNRVYYFLGELPYLTGTESLIYEGITL 2069
            D+IWSLNYFALIVLNFLEKPLWC++      +C +R YY+LG+LPYLTG ESL+YEGITL
Sbjct: 84   DYIWSLNYFALIVLNFLEKPLWCAKTGVGTYSCYDREYYYLGQLPYLTGWESLVYEGITL 143

Query: 2068 LVLIIHIFFPITYEGFGIYWKSXXXXXXXXXXXXXXXXXXXXXXXLSPVAFYTLPFRIAP 1889
            ++LIIH FFPITYEG  I+WKS                       LSP+AF  LP RIAP
Sbjct: 144  VILIIHTFFPITYEGSPIFWKSTYTRLKVLCLLILVADFLVYGLYLSPIAFDFLPLRIAP 203

Query: 1888 YIRVVLFILNIRELRESTVVLAGMLSTYSNVLGLCGFYFFYS 1763
            YIRVV FILNIR+LR++  VLAGML TY NVL L   +  +S
Sbjct: 204  YIRVVFFILNIRQLRDTLFVLAGMLGTYLNVLALGLLFLLFS 245


>ref|XP_011037333.1| PREDICTED: two pore calcium channel protein 1 [Populus euphratica]
          Length = 741

 Score =  704 bits (1816), Expect(2) = 0.0
 Identities = 347/493 (70%), Positives = 384/493 (77%)
 Frame = -2

Query: 1748 FVIFEDTEQGRTLFTSYGTTLYQMFVLFTTSNNPDVWIPAYKSSRWYCLFFILYVLLGVY 1569
            +V+FEDT+QG+ +FTSYGTTLYQMFVLFTTSNNPDVWIPAYK SRWYCLFF+LYVL+GVY
Sbjct: 243  YVMFEDTQQGKIIFTSYGTTLYQMFVLFTTSNNPDVWIPAYKVSRWYCLFFVLYVLIGVY 302

Query: 1568 FVTNLILAVVYDSFKSELVKQVAEKDRVRMRILKKAFNLIDNYDLGYLNKDQCIRLFEEL 1389
            FVTNL+LAVVYDSFK +LVKQV+E D  R RIL KAFNLIDN   GYL+K QCI LFEEL
Sbjct: 303  FVTNLVLAVVYDSFKDQLVKQVSEMDHSRKRILMKAFNLIDNNKAGYLDKKQCIYLFEEL 362

Query: 1388 NKYRTLPKISRXXXXXXXXXXXDSHDFKINLDEFADLVNAIALRFQKEDSLPIFESCPTF 1209
            NKYRTLP IS+           DSHDFKINLDEF DL NAIALRFQKED    FE  P+ 
Sbjct: 363  NKYRTLPNISKEEFELIFDELDDSHDFKINLDEFEDLCNAIALRFQKEDVPSYFEYLPSI 422

Query: 1208 YHSPASEKLKDFVRSPKFGYIIVFILILNFVAVVIETTLDIENNSAQEAWQKVEFVFGWL 1029
            YHS  SEKLK+FVR PKFGYII  ILI+N +AV+IETTLDI NNSAQ+ WQKVEF+FGW+
Sbjct: 423  YHSRFSEKLKEFVRRPKFGYIISLILIMNLIAVIIETTLDIANNSAQKVWQKVEFIFGWI 482

Query: 1028 YVLEMALKVYAYGFENYWRDSQNRFDFIVTWVIVIGETTTFVSPHGLTFLSNGEWXXXXX 849
            YV+EMALK+YAYGFENYWRD QNRFDF++T VIVIGET TF SP+GLTFLSNGEW     
Sbjct: 483  YVVEMALKIYAYGFENYWRDGQNRFDFVITLVIVIGETITFASPNGLTFLSNGEWIRYLL 542

Query: 848  XXXXXXXXXXXXRVERYRAFVTTFLTLIPSLMPYLGIIFCVLCIYCSLGVQIFGGIVNAG 669
                         V  Y+AFV TFL LIPSLMPYLG IFCV+CIYCSLG+Q+FGG+VNAG
Sbjct: 543  IARLLRLIRILMYVRSYKAFVATFLNLIPSLMPYLGTIFCVMCIYCSLGIQVFGGLVNAG 602

Query: 668  NPNLVGTDLSDDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKDLTGTTWSYVYFVS 489
            NPNL  TDL+ DDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQSYKDLTGT WS  YF+S
Sbjct: 603  NPNLEETDLAGDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQSYKDLTGTYWSLAYFIS 662

Query: 488  FYLISXXXXXXXXXXXXXXXXXXXXXXETSEKSEDGVGKEERGKDRRRNVGTKSRSQRVD 309
            FYLI+                      ETSEK E    +    K R R+VGTK+RSQRVD
Sbjct: 663  FYLITILLLLNLVMAFVLEAFFAEMELETSEKCEGADKEGSNSKSRHRSVGTKTRSQRVD 722

Query: 308  ILLHHMLSAELDK 270
            ILLHHMLSAEL+K
Sbjct: 723  ILLHHMLSAELEK 735



 Score =  260 bits (665), Expect(2) = 0.0
 Identities = 134/238 (56%), Positives = 160/238 (67%), Gaps = 4/238 (1%)
 Frame = -3

Query: 2464 METALLXXXXXXXXXXSPRRDRKHIFNRRSDAIAYGSTYQKAAALVDLAADGIGIPEEIL 2285
            ME  LL            RRD    F+RRSDAI +GS  QKAAALVDLA DGIGIPEEIL
Sbjct: 1    MEKPLLGESSGGRDIHFRRRDSTSKFSRRSDAITHGSPIQKAAALVDLAEDGIGIPEEIL 60

Query: 2284 DESSFGSSLKSYFVFIQFDFIWSLNYFALIVLNFLEKPLWCSEN----CSNRVYYFLGEL 2117
            D+++F S  K YF+F+QFD IW+LNYFA+IVLNFLEKPLWC  N    C++R Y++LG+L
Sbjct: 61   DQANFESVAKFYFIFVQFDIIWTLNYFAMIVLNFLEKPLWCQNNSGHSCNDREYFYLGQL 120

Query: 2116 PYLTGTESLIYEGITLLVLIIHIFFPITYEGFGIYWKSXXXXXXXXXXXXXXXXXXXXXX 1937
            PYLT  ES ++EGITL+VL+ HIFFPI+YEGF IYWK+                      
Sbjct: 121  PYLTAAESFVFEGITLIVLVAHIFFPISYEGFRIYWKNPLNWSKVIFLCILVVDLVAYAI 180

Query: 1936 XLSPVAFYTLPFRIAPYIRVVLFILNIRELRESTVVLAGMLSTYSNVLGLCGFYFFYS 1763
              SPVAF  LPFRIAPYIRVV FIL IR+L+ S ++L GML TY NVL L   +  +S
Sbjct: 181  YASPVAFNFLPFRIAPYIRVVFFILYIRDLQRSIIILGGMLKTYVNVLALGLLFLLFS 238


>ref|XP_007043598.1| Two-pore channel 1 [Theobroma cacao] gi|508707533|gb|EOX99429.1|
            Two-pore channel 1 [Theobroma cacao]
          Length = 737

 Score =  709 bits (1829), Expect(2) = 0.0
 Identities = 353/493 (71%), Positives = 388/493 (78%)
 Frame = -2

Query: 1748 FVIFEDTEQGRTLFTSYGTTLYQMFVLFTTSNNPDVWIPAYKSSRWYCLFFILYVLLGVY 1569
            +V+FEDT++G+ +FTSYGTTLYQMFVLFTTSNNPDVWIPAYK+SRWYCLFF+LYVL+GVY
Sbjct: 237  YVMFEDTQEGKLVFTSYGTTLYQMFVLFTTSNNPDVWIPAYKASRWYCLFFVLYVLVGVY 296

Query: 1568 FVTNLILAVVYDSFKSELVKQVAEKDRVRMRILKKAFNLIDNYDLGYLNKDQCIRLFEEL 1389
            FVTNLILAVVYDSFKSEL KQV+E DR R  IL KAF LID+Y +G+LNK+QCIRLFEEL
Sbjct: 297  FVTNLILAVVYDSFKSELAKQVSEMDRTRRSILCKAFGLIDDYKVGFLNKEQCIRLFEEL 356

Query: 1388 NKYRTLPKISRXXXXXXXXXXXDSHDFKINLDEFADLVNAIALRFQKEDSLPIFESCPTF 1209
            NKYRTLPKISR           DSHDFKINLDEF DL NAI LRFQKEDS  +FE  P  
Sbjct: 357  NKYRTLPKISREEFELIFDELDDSHDFKINLDEFTDLCNAIGLRFQKEDSPSLFERFP-I 415

Query: 1208 YHSPASEKLKDFVRSPKFGYIIVFILILNFVAVVIETTLDIENNSAQEAWQKVEFVFGWL 1029
            Y SP SE LK FV+SPKFGYI+  ILILN  AV+IETTLDIENNS Q+ WQ+VEFVFGW+
Sbjct: 416  YRSPFSENLKAFVQSPKFGYIVSIILILNLFAVIIETTLDIENNSGQKVWQEVEFVFGWM 475

Query: 1028 YVLEMALKVYAYGFENYWRDSQNRFDFIVTWVIVIGETTTFVSPHGLTFLSNGEWXXXXX 849
            YVLEMALKVYA+GFENYWRD QNRFDF++TW+IVIGET TF +P GL F SNGEW     
Sbjct: 476  YVLEMALKVYAFGFENYWRDGQNRFDFLITWIIVIGETITFATPDGLDFFSNGEWIRYLL 535

Query: 848  XXXXXXXXXXXXRVERYRAFVTTFLTLIPSLMPYLGIIFCVLCIYCSLGVQIFGGIVNAG 669
                         V  YRAFV TFLTLIPSLMPYLG IFCVLCIYCSLG+QIFGGIVNAG
Sbjct: 536  LARMLRLIRLLMHVRSYRAFVATFLTLIPSLMPYLGTIFCVLCIYCSLGIQIFGGIVNAG 595

Query: 668  NPNLVGTDLSDDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKDLTGTTWSYVYFVS 489
            N NL GTDLSDDDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQSYK+LTGT+W+ VYF+S
Sbjct: 596  NSNLDGTDLSDDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQSYKELTGTSWTLVYFIS 655

Query: 488  FYLISXXXXXXXXXXXXXXXXXXXXXXETSEKSEDGVGKEERGKDRRRNVGTKSRSQRVD 309
            FYLI+                      ETS   E+       GK RRR VGTK+RSQR+D
Sbjct: 656  FYLITVLLLLNLVVAFVLEAFFTEMDLETSGNCEEDDKDAGSGKYRRRLVGTKTRSQRID 715

Query: 308  ILLHHMLSAELDK 270
            ILLHHMLSAELDK
Sbjct: 716  ILLHHMLSAELDK 728



 Score =  251 bits (642), Expect(2) = 0.0
 Identities = 129/221 (58%), Positives = 155/221 (70%), Gaps = 4/221 (1%)
 Frame = -3

Query: 2401 RKHIFNRRSDAIAYGSTYQKAAALVDLAADGIGIPEEILDESSFGSSLKSYFVFIQFDFI 2222
            R+    RRS+AI YGS YQKAAALVDLA DG+G+PE+ILD+SSFGS+ K YF+FI+FD I
Sbjct: 16   RRDPLYRRSEAITYGSPYQKAAALVDLAEDGVGLPEQILDKSSFGSAAKFYFIFIKFDLI 75

Query: 2221 WSLNYFALIVLNFLEKPLWC----SENCSNRVYYFLGELPYLTGTESLIYEGITLLVLII 2054
            W+LNYFALIVLNF EKPLWC    S +CS+R Y+FLG+LPYLT TESLIYE +TLL+ +I
Sbjct: 76   WTLNYFALIVLNFFEKPLWCLRSSSYSCSDREYFFLGQLPYLTSTESLIYEVVTLLLFMI 135

Query: 2053 HIFFPITYEGFGIYWKSXXXXXXXXXXXXXXXXXXXXXXXLSPVAFYTLPFRIAPYIRVV 1874
            H FFPI YEG  IYWK                        LSPV   +LP R+APY+RV+
Sbjct: 136  HTFFPIFYEGSHIYWKRPLNLLKVICLLILVTDLLVYALYLSPVTLTSLPLRVAPYVRVI 195

Query: 1873 LFILNIRELRESTVVLAGMLSTYSNVLGLCGFYFFYSLAGW 1751
            + ILNIRELR S ++LAGM+ TY NVL L  +  F   A W
Sbjct: 196  IVILNIRELRTSILILAGMIKTYLNVLAL--WLLFLLFASW 234


>ref|XP_004243353.1| PREDICTED: two pore calcium channel protein 1B [Solanum lycopersicum]
          Length = 739

 Score =  714 bits (1844), Expect(2) = 0.0
 Identities = 354/491 (72%), Positives = 391/491 (79%)
 Frame = -2

Query: 1748 FVIFEDTEQGRTLFTSYGTTLYQMFVLFTTSNNPDVWIPAYKSSRWYCLFFILYVLLGVY 1569
            +V FEDT+QG+T+FTSYGTTLYQMFVLFTTSNNPDVWIPAYK SRWYCLFF+LYVLLGVY
Sbjct: 242  YVFFEDTQQGKTMFTSYGTTLYQMFVLFTTSNNPDVWIPAYKESRWYCLFFVLYVLLGVY 301

Query: 1568 FVTNLILAVVYDSFKSELVKQVAEKDRVRMRILKKAFNLIDNYDLGYLNKDQCIRLFEEL 1389
            FVTNL+LAVVYDSFKSELVKQVAEKDR ++R LKKAF+LID+ + G L+K QCI LFEEL
Sbjct: 302  FVTNLVLAVVYDSFKSELVKQVAEKDRRKLRTLKKAFSLIDDSNTGVLDKKQCILLFEEL 361

Query: 1388 NKYRTLPKISRXXXXXXXXXXXDSHDFKINLDEFADLVNAIALRFQKEDSLPIFESCPTF 1209
            NKYRTLPKIS            D+ DFKINL+EFADL  AI LRFQKEDSLPIFE+CP F
Sbjct: 362  NKYRTLPKISGDDFESIFNELDDTGDFKINLEEFADLCTAIGLRFQKEDSLPIFEACPNF 421

Query: 1208 YHSPASEKLKDFVRSPKFGYIIVFILILNFVAVVIETTLDIENNSAQEAWQKVEFVFGWL 1029
            YHS ASEKL+DFVR   F YIIV +L++N VAV+IETTLDI+NNS Q  WQKVEF FGWL
Sbjct: 422  YHSAASEKLRDFVRGDTFEYIIVSVLLVNLVAVIIETTLDIQNNSGQTFWQKVEFTFGWL 481

Query: 1028 YVLEMALKVYAYGFENYWRDSQNRFDFIVTWVIVIGETTTFVSPHGLTFLSNGEWXXXXX 849
            YV+EMALKVY YGFENYWRD QN+FDFIVTWVIVIGET TFV+P  LTFLSNGEW     
Sbjct: 482  YVIEMALKVYTYGFENYWRDGQNQFDFIVTWVIVIGETATFVAPDDLTFLSNGEWIRYLL 541

Query: 848  XXXXXXXXXXXXRVERYRAFVTTFLTLIPSLMPYLGIIFCVLCIYCSLGVQIFGGIVNAG 669
                         VERYRAFV TFLTLIPSLMPYLG IFC+LC YCSLG+QIFGGIVNAG
Sbjct: 542  IARMLRLMRLLLHVERYRAFVATFLTLIPSLMPYLGTIFCILCFYCSLGLQIFGGIVNAG 601

Query: 668  NPNLVGTDLSDDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKDLTGTTWSYVYFVS 489
            NPNL  T+LSD+DYLLFNFNDYP+GMVT+FN+LVMGNWQ WMQSYK+LTGT W+YVYFVS
Sbjct: 602  NPNLAQTELSDNDYLLFNFNDYPSGMVTVFNILVMGNWQVWMQSYKELTGTAWTYVYFVS 661

Query: 488  FYLISXXXXXXXXXXXXXXXXXXXXXXETSEKSEDGVGKEERGKDRRRNVGTKSRSQRVD 309
            FYLIS                      E +    DG  KE R  +RRRNVGTK+RSQRVD
Sbjct: 662  FYLISVLWLLNLIVAFVLEAFQAEMDLEAAANCADGDDKESR-SERRRNVGTKTRSQRVD 720

Query: 308  ILLHHMLSAEL 276
             LLHHMLS+EL
Sbjct: 721  FLLHHMLSSEL 731



 Score =  241 bits (614), Expect(2) = 0.0
 Identities = 123/220 (55%), Positives = 156/220 (70%), Gaps = 4/220 (1%)
 Frame = -3

Query: 2410 RRDRKHIFNRRSDAIAYGSTYQKAAALVDLAADGIGIPEEILDESSFGSSLKSYFVFIQF 2231
            RR    +F+ R DAIA+GS YQKAAALVDLA DG+G+PEEIL+ +SF  + + YF+F +F
Sbjct: 21   RRRVGSMFDPR-DAIAHGSAYQKAAALVDLAEDGVGLPEEILEGTSFEKAAQLYFMFTRF 79

Query: 2230 DFIWSLNYFALIVLNFLEKPLWCS----ENCSNRVYYFLGELPYLTGTESLIYEGITLLV 2063
            DF+WSLNY AL+++NF EKPLWCS    E+C+NR YY+LG+LP+LTG ESLIYEG+TLL+
Sbjct: 80   DFLWSLNYLALVLINFFEKPLWCSRHLAESCNNRDYYYLGQLPFLTGAESLIYEGVTLLL 139

Query: 2062 LIIHIFFPITYEGFGIYWKSXXXXXXXXXXXXXXXXXXXXXXXLSPVAFYTLPFRIAPYI 1883
            L+IHI FPI+YEGF +YW+S                       L+   FY LPFRIA Y+
Sbjct: 140  LVIHILFPISYEGFHLYWRSLLNRLKVVLLLILVADIVVYILFLAD--FYYLPFRIASYL 197

Query: 1882 RVVLFILNIRELRESTVVLAGMLSTYSNVLGLCGFYFFYS 1763
            RVV FILNIRELR+S  +LAGM  TY NV+ L   +  +S
Sbjct: 198  RVVFFILNIRELRDSFFILAGMFRTYLNVVALTALFLLFS 237


>ref|XP_006357480.1| PREDICTED: two pore calcium channel protein 1B-like [Solanum
            tuberosum]
          Length = 738

 Score =  712 bits (1837), Expect(2) = 0.0
 Identities = 353/491 (71%), Positives = 390/491 (79%)
 Frame = -2

Query: 1748 FVIFEDTEQGRTLFTSYGTTLYQMFVLFTTSNNPDVWIPAYKSSRWYCLFFILYVLLGVY 1569
            +V FEDT+QG+T+FTSYGTTLYQMFVLFTTSNNPDVWIPAYK SRWYCLFF+LYVLLGVY
Sbjct: 242  YVFFEDTQQGKTMFTSYGTTLYQMFVLFTTSNNPDVWIPAYKESRWYCLFFVLYVLLGVY 301

Query: 1568 FVTNLILAVVYDSFKSELVKQVAEKDRVRMRILKKAFNLIDNYDLGYLNKDQCIRLFEEL 1389
            FVTNL+LAVVYDSFKSELVKQVAEKDR ++R LKKAF+LID+ + G L+K QCI LFEEL
Sbjct: 302  FVTNLVLAVVYDSFKSELVKQVAEKDRRKLRTLKKAFSLIDDSNTGVLDKKQCILLFEEL 361

Query: 1388 NKYRTLPKISRXXXXXXXXXXXDSHDFKINLDEFADLVNAIALRFQKEDSLPIFESCPTF 1209
            NKYRTLPKIS            D+ DFKINL+EFADL  AI LRFQKEDSLPIFE+CP F
Sbjct: 362  NKYRTLPKISGDDFESIFNELDDTGDFKINLEEFADLCTAIGLRFQKEDSLPIFEACPNF 421

Query: 1208 YHSPASEKLKDFVRSPKFGYIIVFILILNFVAVVIETTLDIENNSAQEAWQKVEFVFGWL 1029
            YHS ASEKL+DFVR   F YIIV +L++N VAV+IETTLDI+NNS Q  WQKVEF FGWL
Sbjct: 422  YHSAASEKLRDFVRGDMFEYIIVSVLLVNLVAVIIETTLDIQNNSGQTFWQKVEFTFGWL 481

Query: 1028 YVLEMALKVYAYGFENYWRDSQNRFDFIVTWVIVIGETTTFVSPHGLTFLSNGEWXXXXX 849
            YV+EMALKVY YGFENYWRD QN+FDFIVTWVIVIGET TFV+P  LTFLSNGEW     
Sbjct: 482  YVIEMALKVYTYGFENYWRDGQNQFDFIVTWVIVIGETATFVAPDDLTFLSNGEWIRYLL 541

Query: 848  XXXXXXXXXXXXRVERYRAFVTTFLTLIPSLMPYLGIIFCVLCIYCSLGVQIFGGIVNAG 669
                         VERYRAFV TFLTLIPSLMPYLG IFC+LC YCSLG+QIFGGIVNAG
Sbjct: 542  IARMLRLMRLLLHVERYRAFVATFLTLIPSLMPYLGTIFCILCFYCSLGLQIFGGIVNAG 601

Query: 668  NPNLVGTDLSDDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKDLTGTTWSYVYFVS 489
            NPNL  T+LSD+DYLLFNFNDYP+GMVT+FN+LVMGNWQ WMQSYK+LTGT W+YVYFVS
Sbjct: 602  NPNLAQTELSDNDYLLFNFNDYPSGMVTVFNILVMGNWQVWMQSYKELTGTAWTYVYFVS 661

Query: 488  FYLISXXXXXXXXXXXXXXXXXXXXXXETSEKSEDGVGKEERGKDRRRNVGTKSRSQRVD 309
            FYLIS                      E +    DG  KE    +RRRNVGTK+RSQRVD
Sbjct: 662  FYLISVLWLLNLIVAFVLEAFQAEMDLEAAANCVDGDDKE--SSERRRNVGTKTRSQRVD 719

Query: 308  ILLHHMLSAEL 276
             LLHHMLS+EL
Sbjct: 720  FLLHHMLSSEL 730



 Score =  241 bits (615), Expect(2) = 0.0
 Identities = 124/220 (56%), Positives = 156/220 (70%), Gaps = 4/220 (1%)
 Frame = -3

Query: 2410 RRDRKHIFNRRSDAIAYGSTYQKAAALVDLAADGIGIPEEILDESSFGSSLKSYFVFIQF 2231
            RR    +F+ R DAIA+GS YQKAAALVDLA DG+G+PEEIL+ +SF  + + YF+F +F
Sbjct: 21   RRRVGSMFDPR-DAIAHGSAYQKAAALVDLAEDGVGLPEEILEGTSFEKAAQLYFMFTRF 79

Query: 2230 DFIWSLNYFALIVLNFLEKPLWCS----ENCSNRVYYFLGELPYLTGTESLIYEGITLLV 2063
            DF+WSLNY ALI++NF EKPLWCS    E+C+NR YY+LG+LP+LTG ESLIYEG+TLL+
Sbjct: 80   DFLWSLNYLALILINFFEKPLWCSRHLAESCNNRDYYYLGQLPFLTGAESLIYEGVTLLL 139

Query: 2062 LIIHIFFPITYEGFGIYWKSXXXXXXXXXXXXXXXXXXXXXXXLSPVAFYTLPFRIAPYI 1883
            L+IHI FPI+YEGF +YW+S                       L+   FY LPFRIA Y+
Sbjct: 140  LVIHILFPISYEGFHLYWRSLLNRLKIVLLLILVADIVVYILFLAD--FYYLPFRIASYL 197

Query: 1882 RVVLFILNIRELRESTVVLAGMLSTYSNVLGLCGFYFFYS 1763
            RVV FILNIRELR+S  +LAGM  TY NV+ L   +  +S
Sbjct: 198  RVVFFILNIRELRDSFFILAGMFRTYLNVVALTALFLLFS 237


>ref|XP_006447308.1| hypothetical protein CICLE_v10014388mg [Citrus clementina]
            gi|557549919|gb|ESR60548.1| hypothetical protein
            CICLE_v10014388mg [Citrus clementina]
          Length = 722

 Score =  687 bits (1774), Expect(2) = 0.0
 Identities = 339/484 (70%), Positives = 382/484 (78%)
 Frame = -2

Query: 1721 GRTLFTSYGTTLYQMFVLFTTSNNPDVWIPAYKSSRWYCLFFILYVLLGVYFVTNLILAV 1542
            G  +FTS+GTTLYQMFVLFTTSNNPDVWIPAYK+SRWYCLFF+L+VL+GVYFVTNLILAV
Sbjct: 236  GNMVFTSFGTTLYQMFVLFTTSNNPDVWIPAYKASRWYCLFFVLFVLIGVYFVTNLILAV 295

Query: 1541 VYDSFKSELVKQVAEKDRVRMRILKKAFNLIDNYDLGYLNKDQCIRLFEELNKYRTLPKI 1362
            VYDSFKS+L KQV+E DR+R R L KAFNLIDNY++G+LNK+QCI+LFEELNKYRTLP I
Sbjct: 296  VYDSFKSQLAKQVSEMDRMRRRTLGKAFNLIDNYNVGFLNKEQCIKLFEELNKYRTLPNI 355

Query: 1361 SRXXXXXXXXXXXDSHDFKINLDEFADLVNAIALRFQKEDSLPIFESCPTFYHSPASEKL 1182
            SR           D+HDFKINLDEFADL NAIALRFQKED    FE+ P+ YHSP SEKL
Sbjct: 356  SREEFELIFDELDDTHDFKINLDEFADLCNAIALRFQKEDVPSCFENLPSIYHSPFSEKL 415

Query: 1181 KDFVRSPKFGYIIVFILILNFVAVVIETTLDIENNSAQEAWQKVEFVFGWLYVLEMALKV 1002
            K F+RS KFGY+I  ILI+N VAV++ETTLDI+ +S Q  WQ+VEFVFGW+YVLEMALK+
Sbjct: 416  KAFIRSTKFGYMISIILIVNLVAVIVETTLDIQESSLQSVWQEVEFVFGWIYVLEMALKI 475

Query: 1001 YAYGFENYWRDSQNRFDFIVTWVIVIGETTTFVSPHGLTFLSNGEWXXXXXXXXXXXXXX 822
            Y+YGFENYWRD QNRFDF+VTWVIVIGET T  SP+G TFLSNGEW              
Sbjct: 476  YSYGFENYWRDGQNRFDFLVTWVIVIGETITLASPNGQTFLSNGEWIRYLLLARMLRLIR 535

Query: 821  XXXRVERYRAFVTTFLTLIPSLMPYLGIIFCVLCIYCSLGVQIFGGIVNAGNPNLVGTDL 642
                V++YR FV TFLTLIPSLMPYLG IFCV CIYCSLGVQIFGGIVNAGN  L  TDL
Sbjct: 536  LLMHVQQYRGFVATFLTLIPSLMPYLGTIFCVQCIYCSLGVQIFGGIVNAGNAKLEETDL 595

Query: 641  SDDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKDLTGTTWSYVYFVSFYLISXXXX 462
            +DDDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQSYK+LTGT W+  YFVSFYLI+    
Sbjct: 596  ADDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQSYKELTGTAWTLAYFVSFYLITVLLL 655

Query: 461  XXXXXXXXXXXXXXXXXXETSEKSEDGVGKEERGKDRRRNVGTKSRSQRVDILLHHMLSA 282
                              E+SEK E+   K+   ++RRR VGTK+RSQRVD+LLHHMLSA
Sbjct: 656  LNLVIAFVLEAFFAEMELESSEKCEE-EDKDGEPRERRRRVGTKTRSQRVDVLLHHMLSA 714

Query: 281  ELDK 270
            EL K
Sbjct: 715  ELQK 718



 Score =  265 bits (677), Expect(2) = 0.0
 Identities = 139/220 (63%), Positives = 159/220 (72%), Gaps = 6/220 (2%)
 Frame = -3

Query: 2410 RRDRKHIFNRRSDAIAYGSTYQKAAALVDLAADGIGIPEEILDESSFGSSLKSYFVFIQF 2231
            RR    +F RR+DAI  GS YQKAAALVDLA DGIG+PEEILD SSF SS K YF+FI+F
Sbjct: 24   RRGPTALFRRRTDAITRGSPYQKAAALVDLAEDGIGLPEEILDRSSFESSAKFYFIFIKF 83

Query: 2230 DFIWSLNYFALIVLNFLEKPLWCSE------NCSNRVYYFLGELPYLTGTESLIYEGITL 2069
            D+IWSLNYFALIVLNFLEKPLWC++      +C +R YY+LG+LPYLTG ESL+YEGITL
Sbjct: 84   DYIWSLNYFALIVLNFLEKPLWCAKTGVGTYSCYDREYYYLGQLPYLTGWESLVYEGITL 143

Query: 2068 LVLIIHIFFPITYEGFGIYWKSXXXXXXXXXXXXXXXXXXXXXXXLSPVAFYTLPFRIAP 1889
            ++LIIH FFPITYEG  I+WKS                       LSP+AF  LP RIAP
Sbjct: 144  VILIIHTFFPITYEGSPIFWKSTYTRLKVLCLLILVADFLVYGLYLSPIAFDFLPLRIAP 203

Query: 1888 YIRVVLFILNIRELRESTVVLAGMLSTYSNVLGLCGFYFF 1769
            YIRVV FILNIR+LR++  VLAGML TY NVLG   F  F
Sbjct: 204  YIRVVFFILNIRQLRDTLFVLAGMLGTYLNVLGNMVFTSF 243


>ref|XP_012479695.1| PREDICTED: two pore calcium channel protein 1 isoform X1 [Gossypium
            raimondii] gi|763764433|gb|KJB31687.1| hypothetical
            protein B456_005G201400 [Gossypium raimondii]
          Length = 736

 Score =  688 bits (1776), Expect(2) = 0.0
 Identities = 343/493 (69%), Positives = 382/493 (77%)
 Frame = -2

Query: 1748 FVIFEDTEQGRTLFTSYGTTLYQMFVLFTTSNNPDVWIPAYKSSRWYCLFFILYVLLGVY 1569
            +V+FEDT+QG+ +FTSYGTTLYQMFVLFTTSNNPDVWIPAYK+SRWYCLFF+L+VL+GVY
Sbjct: 236  YVMFEDTKQGKLVFTSYGTTLYQMFVLFTTSNNPDVWIPAYKASRWYCLFFVLFVLIGVY 295

Query: 1568 FVTNLILAVVYDSFKSELVKQVAEKDRVRMRILKKAFNLIDNYDLGYLNKDQCIRLFEEL 1389
            FVTNLILAVVYDSFKSEL KQV+E DR R  IL KAF+LID+Y++G+LNK+QCI LFEEL
Sbjct: 296  FVTNLILAVVYDSFKSELAKQVSEMDRNRRSILCKAFDLIDDYNVGFLNKEQCIHLFEEL 355

Query: 1388 NKYRTLPKISRXXXXXXXXXXXDSHDFKINLDEFADLVNAIALRFQKEDSLPIFESCPTF 1209
            NKYR+LPKISR           DSHDFKINLDEF DL NAI LRFQKED   +FE     
Sbjct: 356  NKYRSLPKISREEFELIFDELDDSHDFKINLDEFTDLCNAIGLRFQKEDVPSLFERF-QI 414

Query: 1208 YHSPASEKLKDFVRSPKFGYIIVFILILNFVAVVIETTLDIENNSAQEAWQKVEFVFGWL 1029
            Y SP SE LK FVR PKFGYII FILILN  AV+IETTLDI NNS Q+ WQ+VEFVFGW+
Sbjct: 415  YRSPFSENLKAFVRGPKFGYIISFILILNLFAVIIETTLDIANNSGQKVWQEVEFVFGWI 474

Query: 1028 YVLEMALKVYAYGFENYWRDSQNRFDFIVTWVIVIGETTTFVSPHGLTFLSNGEWXXXXX 849
            YVLEMALKVY++GFENYWRD QNRFDF++TW+IVIGET TF SP    F SNGEW     
Sbjct: 475  YVLEMALKVYSFGFENYWRDGQNRFDFLITWIIVIGETVTFASPDEFYFFSNGEWIRYLL 534

Query: 848  XXXXXXXXXXXXRVERYRAFVTTFLTLIPSLMPYLGIIFCVLCIYCSLGVQIFGGIVNAG 669
                         V  YRAFV TFLTLIPSLMPYLG IFCVLCIYCS+GVQIFGGIVNAG
Sbjct: 535  LARLLRLIRLLMHVRSYRAFVATFLTLIPSLMPYLGTIFCVLCIYCSIGVQIFGGIVNAG 594

Query: 668  NPNLVGTDLSDDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKDLTGTTWSYVYFVS 489
            NP L  T+L+DDDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQSYK+LTGT+WS VYF+S
Sbjct: 595  NPALEATELTDDDYLLFNFNDYPNGMVTLFNLLVMGNWQVWMQSYKELTGTSWSLVYFIS 654

Query: 488  FYLISXXXXXXXXXXXXXXXXXXXXXXETSEKSEDGVGKEERGKDRRRNVGTKSRSQRVD 309
            FYL++                      E S  SE+       GK R+R  GTK+RSQRVD
Sbjct: 655  FYLVTVLLLLNLVVAFVLEAFFAEMDLEASGNSEEDDKDAGSGKGRKRLAGTKTRSQRVD 714

Query: 308  ILLHHMLSAELDK 270
            ILLHHMLSAELD+
Sbjct: 715  ILLHHMLSAELDR 727



 Score =  258 bits (658), Expect(2) = 0.0
 Identities = 133/223 (59%), Positives = 161/223 (72%), Gaps = 3/223 (1%)
 Frame = -3

Query: 2410 RRDRKHIFNRRSDAIAYGSTYQKAAALVDLAADGIGIPEEILDESSFGSSLKSYFVFIQF 2231
            RRDR +   RRS+AI YGS YQ+AAALVDLA DG+G+PE+ILD+SSFGS+ K YF+FI+F
Sbjct: 16   RRDRLY---RRSEAITYGSPYQRAAALVDLAEDGVGLPEQILDKSSFGSAAKFYFMFIKF 72

Query: 2230 DFIWSLNYFALIVLNFLEKPLWC---SENCSNRVYYFLGELPYLTGTESLIYEGITLLVL 2060
            D IW+LNYFAL+VLNFLEKPLWC   S +CS+R Y+FLG+LPYL  TESLIYE +TL++L
Sbjct: 73   DIIWTLNYFALVVLNFLEKPLWCLSSSYSCSDRDYFFLGQLPYLNATESLIYEVVTLILL 132

Query: 2059 IIHIFFPITYEGFGIYWKSXXXXXXXXXXXXXXXXXXXXXXXLSPVAFYTLPFRIAPYIR 1880
             IH  FPI+YEG  IYWK                        LSPVA  +LPFR+APY+R
Sbjct: 133  AIHTIFPISYEGSSIYWKRPLNQIKVICLLILVADLLVYTLYLSPVAMNSLPFRVAPYVR 192

Query: 1879 VVLFILNIRELRESTVVLAGMLSTYSNVLGLCGFYFFYSLAGW 1751
            V+LFIL+IRELR S V+LAGM+ TY NVL L  +  F   A W
Sbjct: 193  VILFILSIRELRNSIVILAGMIGTYLNVLAL--WLLFLLFASW 233


>ref|XP_008455316.1| PREDICTED: two pore calcium channel protein 1B isoform X1 [Cucumis
            melo]
          Length = 743

 Score =  682 bits (1760), Expect(2) = 0.0
 Identities = 332/491 (67%), Positives = 387/491 (78%)
 Frame = -2

Query: 1748 FVIFEDTEQGRTLFTSYGTTLYQMFVLFTTSNNPDVWIPAYKSSRWYCLFFILYVLLGVY 1569
            +VIFEDT+QG+ +FTS+GTTLYQMF+LFTTSNNP+VWIPAYK+SRWYCLFF+LYVLLGVY
Sbjct: 247  YVIFEDTQQGKLIFTSFGTTLYQMFILFTTSNNPNVWIPAYKASRWYCLFFVLYVLLGVY 306

Query: 1568 FVTNLILAVVYDSFKSELVKQVAEKDRVRMRILKKAFNLIDNYDLGYLNKDQCIRLFEEL 1389
            FVTNLILAVVYDSFKS+L KQ++E DR+R  IL KAF+L+D  ++G++NK+QC+ L EEL
Sbjct: 307  FVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGFINKEQCLMLLEEL 366

Query: 1388 NKYRTLPKISRXXXXXXXXXXXDSHDFKINLDEFADLVNAIALRFQKEDSLPIFESCPTF 1209
            N+YRTLPKISR           DS DFKIN++EF DL NAIAL+FQKED    FE  P+ 
Sbjct: 367  NRYRTLPKISRDDYELIFDELDDSRDFKININEFDDLCNAIALKFQKEDIPSWFEGYPSI 426

Query: 1208 YHSPASEKLKDFVRSPKFGYIIVFILILNFVAVVIETTLDIENNSAQEAWQKVEFVFGWL 1029
            YHS  S+KLK FVRSPKFGY + FILILN VAV+IETTLDIENN  Q+AWQ++EFVFGW+
Sbjct: 427  YHSSFSKKLKAFVRSPKFGYAVSFILILNLVAVIIETTLDIENNVGQKAWQELEFVFGWI 486

Query: 1028 YVLEMALKVYAYGFENYWRDSQNRFDFIVTWVIVIGETTTFVSPHGLTFLSNGEWXXXXX 849
            YV+EMALK+YAYGF NYWRD QN+FDF++TW+IVIGET TFV+P GLTFLSNGEW     
Sbjct: 487  YVVEMALKIYAYGFMNYWRDGQNQFDFLITWIIVIGETITFVAPSGLTFLSNGEWIRYLL 546

Query: 848  XXXXXXXXXXXXRVERYRAFVTTFLTLIPSLMPYLGIIFCVLCIYCSLGVQIFGGIVNAG 669
                         V +YRAF+ TFLTLIPSLMPYLG IFCVLC+YC+LGVQ+FGGIVNAG
Sbjct: 547  IARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAG 606

Query: 668  NPNLVGTDLSDDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKDLTGTTWSYVYFVS 489
            NP L  TDL DDDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQSYK+LTG+ WS VYF+S
Sbjct: 607  NPTLPKTDLDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYKELTGSIWSLVYFIS 666

Query: 488  FYLISXXXXXXXXXXXXXXXXXXXXXXETSEKSEDGVGKEERGKDRRRNVGTKSRSQRVD 309
            FYLI+                      E+SE  E+     +  KDR R VGTK+RS++VD
Sbjct: 667  FYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDSDSRKDRPRFVGTKTRSRKVD 726

Query: 308  ILLHHMLSAEL 276
            ILLHHMLSAEL
Sbjct: 727  ILLHHMLSAEL 737



 Score =  261 bits (668), Expect(2) = 0.0
 Identities = 132/225 (58%), Positives = 163/225 (72%), Gaps = 5/225 (2%)
 Frame = -3

Query: 2410 RRDRKHI-FNRRSDAIAYGSTYQKAAALVDLAADGIGIPEEILDESSFGSSLKSYFVFIQ 2234
            RR   H+ F RRSDAIAYGS YQ+AAALVDLA DG+GIPE+ILD+S+F S+ K YF+FI+
Sbjct: 22   RRQTPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGVGIPEKILDQSNFQSAAKLYFIFIK 81

Query: 2233 FDFIWSLNYFALIVLNFLEKPLWCSE----NCSNRVYYFLGELPYLTGTESLIYEGITLL 2066
            FD+IWSLN+FALIVLNF EKPLWCS+    +C++R Y+FLG+LPYLT  ESLIYE +TL+
Sbjct: 82   FDWIWSLNFFALIVLNFFEKPLWCSKYDTHSCNDREYFFLGQLPYLTAVESLIYEAVTLI 141

Query: 2065 VLIIHIFFPITYEGFGIYWKSXXXXXXXXXXXXXXXXXXXXXXXLSPVAFYTLPFRIAPY 1886
            +L+IH FFPI+YEG  +YW +                       LSPVAF  LPFR APY
Sbjct: 142  ILMIHTFFPISYEGAQLYWTNLLNQLKVICIIILVADFLVYTLYLSPVAFDYLPFRAAPY 201

Query: 1885 IRVVLFILNIRELRESTVVLAGMLSTYSNVLGLCGFYFFYSLAGW 1751
            IRV+ FI+NIR LRES ++LAGML TY NVL L  ++ F   + W
Sbjct: 202  IRVIFFIMNIRHLRESIIILAGMLGTYLNVLAL--WFLFLLFSSW 244


>ref|XP_012088278.1| PREDICTED: two pore calcium channel protein 1 [Jatropha curcas]
            gi|643709713|gb|KDP24122.1| hypothetical protein
            JCGZ_25779 [Jatropha curcas]
          Length = 735

 Score =  687 bits (1772), Expect(2) = 0.0
 Identities = 338/491 (68%), Positives = 385/491 (78%)
 Frame = -2

Query: 1748 FVIFEDTEQGRTLFTSYGTTLYQMFVLFTTSNNPDVWIPAYKSSRWYCLFFILYVLLGVY 1569
            +V+FEDT+QG+T+FTSYGTTLY+MFVLFTTSNNPDVWIPAYK+SRWY LFF+LYVLLGVY
Sbjct: 241  YVMFEDTQQGKTVFTSYGTTLYEMFVLFTTSNNPDVWIPAYKASRWYSLFFVLYVLLGVY 300

Query: 1568 FVTNLILAVVYDSFKSELVKQVAEKDRVRMRILKKAFNLIDNYDLGYLNKDQCIRLFEEL 1389
            FVTNLILAVVYDSFKS+LVKQV+E D  R R+L KAFNL+D  ++GYL KDQCI LFEEL
Sbjct: 301  FVTNLILAVVYDSFKSQLVKQVSEADCTRKRMLVKAFNLLDKNNVGYLKKDQCILLFEEL 360

Query: 1388 NKYRTLPKISRXXXXXXXXXXXDSHDFKINLDEFADLVNAIALRFQKEDSLPIFESCPTF 1209
            NKYRTLPKIS+           DSHDFKINLDEF DL NAIALRF KED   +FE+ P  
Sbjct: 361  NKYRTLPKISKEEFQLIFDELDDSHDFKINLDEFTDLCNAIALRFLKEDVPSVFETFPLV 420

Query: 1208 YHSPASEKLKDFVRSPKFGYIIVFILILNFVAVVIETTLDIENNSAQEAWQKVEFVFGWL 1029
            Y S  SE LK FVRSP+FGY+I FIL++N +AV+IETTLDI+NNSAQ+ WQ VEFVFGW+
Sbjct: 421  YRSRFSENLKAFVRSPRFGYLISFILVVNLIAVIIETTLDIQNNSAQKVWQVVEFVFGWI 480

Query: 1028 YVLEMALKVYAYGFENYWRDSQNRFDFIVTWVIVIGETTTFVSPHGLTFLSNGEWXXXXX 849
            YVLEMALK+Y++GFENYWRD QNRFDF++TWVIVIGET TF SPHGLTFLS+GEW     
Sbjct: 481  YVLEMALKIYSFGFENYWRDGQNRFDFLITWVIVIGETITFASPHGLTFLSDGEWIRYLL 540

Query: 848  XXXXXXXXXXXXRVERYRAFVTTFLTLIPSLMPYLGIIFCVLCIYCSLGVQIFGGIVNAG 669
                         V+ YRAFV TFLTLIPSLMPYLG IFCVLCIYCSLG+QIFGG+VNAG
Sbjct: 541  LARMLRLIRLLMHVQSYRAFVATFLTLIPSLMPYLGTIFCVLCIYCSLGIQIFGGLVNAG 600

Query: 668  NPNLVGTDLSDDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKDLTGTTWSYVYFVS 489
            NP+L  TDL++ DYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQ YK L G++W+ VYF+S
Sbjct: 601  NPDLESTDLAESDYLLFNFNDYPNGMVTLFNLLVMGNWQIWMQGYKQLAGSSWTLVYFIS 660

Query: 488  FYLISXXXXXXXXXXXXXXXXXXXXXXETSEKSEDGVGKEERGKDRRRNVGTKSRSQRVD 309
            FYLI+                      E  E+ ED    E+    + R +GTKSRSQRVD
Sbjct: 661  FYLITVLLLLNLVVAFVLEAFFAEMDLEKPEECED----EDARTSKPRRIGTKSRSQRVD 716

Query: 308  ILLHHMLSAEL 276
            ILLHHMLSAEL
Sbjct: 717  ILLHHMLSAEL 727



 Score =  255 bits (651), Expect(2) = 0.0
 Identities = 131/220 (59%), Positives = 156/220 (70%), Gaps = 4/220 (1%)
 Frame = -3

Query: 2410 RRDRKHIFNRRSDAIAYGSTYQKAAALVDLAADGIGIPEEILDESSFGSSLKSYFVFIQF 2231
            RRDR   FNRRSDAI YGS YQKAAALVDLA DG+G+PEEIL +S+F S+ K YF++ QF
Sbjct: 17   RRDRTGTFNRRSDAITYGSRYQKAAALVDLAEDGVGLPEEILGQSNFESAAKLYFIYTQF 76

Query: 2230 DFIWSLNYFALIVLNFLEKPLWC----SENCSNRVYYFLGELPYLTGTESLIYEGITLLV 2063
            D +W+LNYFALIVL+FLEKPLWC      +C++R Y++LG+LPYLT TESLIYEGITL++
Sbjct: 77   DIVWTLNYFALIVLSFLEKPLWCVNYTVHSCNDRDYFYLGQLPYLTATESLIYEGITLII 136

Query: 2062 LIIHIFFPITYEGFGIYWKSXXXXXXXXXXXXXXXXXXXXXXXLSPVAFYTLPFRIAPYI 1883
            L  HIF+PI YEG  I+WK+                       LSPVA   LP RIAPYI
Sbjct: 137  LAAHIFYPILYEGSRIFWKNPLNLFKVFCLLILLADLLVYVLYLSPVAIDYLPVRIAPYI 196

Query: 1882 RVVLFILNIRELRESTVVLAGMLSTYSNVLGLCGFYFFYS 1763
            RVV FIL IREL+ S V+LAGML TY NVL L   +  +S
Sbjct: 197  RVVFFILFIRELQNSIVILAGMLKTYLNVLALLLLFLLFS 236


>ref|XP_006376490.1| calcium channel 1 family protein [Populus trichocarpa]
            gi|550325766|gb|ERP54287.1| calcium channel 1 family
            protein [Populus trichocarpa]
          Length = 726

 Score =  690 bits (1781), Expect(2) = 0.0
 Identities = 345/493 (69%), Positives = 382/493 (77%)
 Frame = -2

Query: 1748 FVIFEDTEQGRTLFTSYGTTLYQMFVLFTTSNNPDVWIPAYKSSRWYCLFFILYVLLGVY 1569
            FV+FEDT+QG+ +FTSYG TLYQMFVLFTTSNNPDVWIPAYK SRWY LFFILYVL+GVY
Sbjct: 228  FVMFEDTQQGKIVFTSYGITLYQMFVLFTTSNNPDVWIPAYKVSRWYSLFFILYVLIGVY 287

Query: 1568 FVTNLILAVVYDSFKSELVKQVAEKDRVRMRILKKAFNLIDNYDLGYLNKDQCIRLFEEL 1389
            FVTNL+LAVVYDSFK +LVKQV+E DR R RIL KAFNLID  D GYLNK+QCI LFE L
Sbjct: 288  FVTNLVLAVVYDSFKDQLVKQVSEMDRSRKRILIKAFNLIDKNDAGYLNKEQCIHLFEAL 347

Query: 1388 NKYRTLPKISRXXXXXXXXXXXDSHDFKINLDEFADLVNAIALRFQKEDSLPIFESCPTF 1209
            NKYRTLP ISR           DSHDFKINLDEF DL NAIALRFQKED    FE  P+ 
Sbjct: 348  NKYRTLPSISREEFELIFDELDDSHDFKINLDEFEDLCNAIALRFQKEDVPSYFEYLPSI 407

Query: 1208 YHSPASEKLKDFVRSPKFGYIIVFILILNFVAVVIETTLDIENNSAQEAWQKVEFVFGWL 1029
            YHS  SEKLK+FVRSPKFGYII  IL++N +AV+ ETTLDI NNSAQ+ WQ+VEFVFGW+
Sbjct: 408  YHSLFSEKLKEFVRSPKFGYIISSILVMNLLAVITETTLDIANNSAQKVWQEVEFVFGWI 467

Query: 1028 YVLEMALKVYAYGFENYWRDSQNRFDFIVTWVIVIGETTTFVSPHGLTFLSNGEWXXXXX 849
            YV+EMALK+YAYGFENYWRD QNRFDF++T VIVIGET TF SP+ LTFLSNGEW     
Sbjct: 468  YVVEMALKIYAYGFENYWRDGQNRFDFVITLVIVIGETVTFASPNELTFLSNGEWIRYLL 527

Query: 848  XXXXXXXXXXXXRVERYRAFVTTFLTLIPSLMPYLGIIFCVLCIYCSLGVQIFGGIVNAG 669
                         V  YRAFV TFLTLIPSLMPYLG IFCV+CIYCSLG+QIFGG+VNAG
Sbjct: 528  IARLLRLIRILMYVRSYRAFVATFLTLIPSLMPYLGTIFCVMCIYCSLGIQIFGGLVNAG 587

Query: 668  NPNLVGTDLSDDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKDLTGTTWSYVYFVS 489
            N +L GT L++DDYLLFNFNDYPNGMVTLFNLLVMGNW  WMQSYKDLTGT WS  YF+S
Sbjct: 588  NLDLEGTGLAEDDYLLFNFNDYPNGMVTLFNLLVMGNWHEWMQSYKDLTGTYWSLAYFIS 647

Query: 488  FYLISXXXXXXXXXXXXXXXXXXXXXXETSEKSEDGVGKEERGKDRRRNVGTKSRSQRVD 309
            FYL++                      ET+EK E    +    K RRR+VGTK+RSQRVD
Sbjct: 648  FYLVTVLLLLNLVMAFVLEGFFAEMELETAEKCEAEDKEGSNSKSRRRSVGTKTRSQRVD 707

Query: 308  ILLHHMLSAELDK 270
             LLHHMLSAEL+K
Sbjct: 708  NLLHHMLSAELEK 720



 Score =  249 bits (637), Expect(2) = 0.0
 Identities = 136/236 (57%), Positives = 159/236 (67%), Gaps = 5/236 (2%)
 Frame = -3

Query: 2464 METALLXXXXXXXXXXSPRRDRKHI-FNRRSDAIAYGSTYQKAAALVDLAADGIGIPEEI 2288
            ME  LL            RRD+    F RRSDAI +GS +QKAAALVDLA DGIGIPEEI
Sbjct: 1    MEKPLLGESSGGRDIRFRRRDQSTPKFPRRSDAITHGSPFQKAAALVDLAEDGIGIPEEI 60

Query: 2287 LDESSFGSSLKSYFVFIQFDFIWSLNYFALIVLNFLEKPLWCSEN----CSNRVYYFLGE 2120
            LD+++F S  K YF+FIQFD IW+LNYFA+IVLNFLEKPLWC  N    C++R Y++LGE
Sbjct: 61   LDQANFESVAKFYFIFIQFDIIWTLNYFAMIVLNFLEKPLWCQNNSGHSCNDREYFYLGE 120

Query: 2119 LPYLTGTESLIYEGITLLVLIIHIFFPITYEGFGIYWKSXXXXXXXXXXXXXXXXXXXXX 1940
            LPYLT  ES ++EGITL++L  HIFFPI+YEGF IYWK+                     
Sbjct: 121  LPYLTAAESFVFEGITLIILAAHIFFPISYEGFRIYWKNPLNCSKVLGY----------- 169

Query: 1939 XXLSPVAFYTLPFRIAPYIRVVLFILNIRELRESTVVLAGMLSTYSNVLGLCGFYF 1772
                 VAF  LPFRIAPYIRVVLFIL IR+L+ S ++L GML TY NVL L GF F
Sbjct: 170  -----VAFNFLPFRIAPYIRVVLFILYIRDLQRSIIILGGMLKTYVNVLAL-GFLF 219


>ref|XP_004136825.1| PREDICTED: two pore calcium channel protein 1A [Cucumis sativus]
            gi|700188384|gb|KGN43617.1| hypothetical protein
            Csa_7G048010 [Cucumis sativus]
          Length = 738

 Score =  681 bits (1756), Expect(2) = 0.0
 Identities = 330/492 (67%), Positives = 389/492 (79%)
 Frame = -2

Query: 1748 FVIFEDTEQGRTLFTSYGTTLYQMFVLFTTSNNPDVWIPAYKSSRWYCLFFILYVLLGVY 1569
            +VIFEDT+QG+ +FTS+G TLYQMF+LFTTSNNP+VWIPAYK+SRWYCLFF+LYVLLGVY
Sbjct: 242  YVIFEDTQQGKLIFTSFGATLYQMFILFTTSNNPNVWIPAYKASRWYCLFFVLYVLLGVY 301

Query: 1568 FVTNLILAVVYDSFKSELVKQVAEKDRVRMRILKKAFNLIDNYDLGYLNKDQCIRLFEEL 1389
            FVTNLILAVVYDSFKS+L KQ++E DR+R  IL KAF+L+D  ++GY+NK+QC+ L EEL
Sbjct: 302  FVTNLILAVVYDSFKSQLAKQISEMDRLRKSILAKAFSLMDTNNVGYINKEQCLMLLEEL 361

Query: 1388 NKYRTLPKISRXXXXXXXXXXXDSHDFKINLDEFADLVNAIALRFQKEDSLPIFESCPTF 1209
            N+YR+LPKISR           DS DFKIN++EF DL NAIAL+FQKED    FE  P+ 
Sbjct: 362  NRYRSLPKISRDDYELIFDELDDSRDFKINMNEFDDLCNAIALKFQKEDIPSWFEGYPSV 421

Query: 1208 YHSPASEKLKDFVRSPKFGYIIVFILILNFVAVVIETTLDIENNSAQEAWQKVEFVFGWL 1029
            YHS +S+KLK FVRSPKFGY + FIL+LN VAV+IETTLDIENN  Q+AWQ++EFVFGW+
Sbjct: 422  YHSGSSKKLKAFVRSPKFGYAVSFILVLNLVAVIIETTLDIENNVGQKAWQELEFVFGWI 481

Query: 1028 YVLEMALKVYAYGFENYWRDSQNRFDFIVTWVIVIGETTTFVSPHGLTFLSNGEWXXXXX 849
            YV+EMALK+YAYGF NYWRD QN+FDF+VTW+IVIGET TFV+P GLTFLSNGEW     
Sbjct: 482  YVVEMALKIYAYGFMNYWRDGQNQFDFLVTWIIVIGETITFVAPSGLTFLSNGEWIRYLL 541

Query: 848  XXXXXXXXXXXXRVERYRAFVTTFLTLIPSLMPYLGIIFCVLCIYCSLGVQIFGGIVNAG 669
                         V +YRAF+ TFLTLIPSLMPYLG IFCVLC+YC+LGVQ+FGGIVNAG
Sbjct: 542  IARMLRLIRLLMHVRQYRAFLATFLTLIPSLMPYLGTIFCVLCLYCTLGVQVFGGIVNAG 601

Query: 668  NPNLVGTDLSDDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKDLTGTTWSYVYFVS 489
            NP L  T+L DDDYLLFNFNDYPNGMVTLFNLLVMGNWQ WMQSY++LTG+ WS VYF+S
Sbjct: 602  NPTLSKTELDDDDYLLFNFNDYPNGMVTLFNLLVMGNWQDWMQSYRELTGSIWSLVYFIS 661

Query: 488  FYLISXXXXXXXXXXXXXXXXXXXXXXETSEKSEDGVGKEERGKDRRRNVGTKSRSQRVD 309
            FYLI+                      E+SE  E+    ++  KDR R VGTK+RS++VD
Sbjct: 662  FYLITVLLLLNLVVAFVLEAFFAELDIESSENGEEQDQDKDSRKDRPRFVGTKTRSRKVD 721

Query: 308  ILLHHMLSAELD 273
            ILLHHMLSAELD
Sbjct: 722  ILLHHMLSAELD 733



 Score =  259 bits (661), Expect(2) = 0.0
 Identities = 131/222 (59%), Positives = 161/222 (72%), Gaps = 5/222 (2%)
 Frame = -3

Query: 2401 RKHI-FNRRSDAIAYGSTYQKAAALVDLAADGIGIPEEILDESSFGSSLKSYFVFIQFDF 2225
            R H+ F RRSDAIAYGS YQ+AAALVDLA DG+GIPE+ILD+S+F S+ K YF+FI+FD+
Sbjct: 20   RPHVRFRRRSDAIAYGSAYQRAAALVDLAEDGVGIPEKILDQSNFQSAAKLYFIFIKFDW 79

Query: 2224 IWSLNYFALIVLNFLEKPLWC----SENCSNRVYYFLGELPYLTGTESLIYEGITLLVLI 2057
            IWSLN+FALIVLNF EKPLWC    + +C++R Y+FLG+LPYLT  ESLIYE +TL++L+
Sbjct: 80   IWSLNFFALIVLNFFEKPLWCFKYDTHSCNDREYFFLGQLPYLTAVESLIYEAVTLIILM 139

Query: 2056 IHIFFPITYEGFGIYWKSXXXXXXXXXXXXXXXXXXXXXXXLSPVAFYTLPFRIAPYIRV 1877
            IH FFPI+YEG  +YW +                       LSPVAF  LPFR APYIRV
Sbjct: 140  IHTFFPISYEGAQLYWTNLLNQLKVICITILVADFLVYTLYLSPVAFDYLPFRAAPYIRV 199

Query: 1876 VLFILNIRELRESTVVLAGMLSTYSNVLGLCGFYFFYSLAGW 1751
            V FI+NIR LRES ++LAGML TY NVL L  ++ F   + W
Sbjct: 200  VFFIMNIRHLRESIIILAGMLGTYLNVLAL--WFLFLLFSSW 239


>ref|XP_007198932.1| hypothetical protein PRUPE_ppa001980mg [Prunus persica]
            gi|462394227|gb|EMJ00131.1| hypothetical protein
            PRUPE_ppa001980mg [Prunus persica]
          Length = 734

 Score =  687 bits (1772), Expect(2) = 0.0
 Identities = 335/491 (68%), Positives = 387/491 (78%)
 Frame = -2

Query: 1748 FVIFEDTEQGRTLFTSYGTTLYQMFVLFTTSNNPDVWIPAYKSSRWYCLFFILYVLLGVY 1569
            +V+FEDT+QG+ +FTSYGTTLYQMFVLFTTSNNPDVWIPAYK+SRWY LFF+LYVLLGVY
Sbjct: 236  YVMFEDTQQGKIMFTSYGTTLYQMFVLFTTSNNPDVWIPAYKASRWYSLFFVLYVLLGVY 295

Query: 1568 FVTNLILAVVYDSFKSELVKQVAEKDRVRMRILKKAFNLIDNYDLGYLNKDQCIRLFEEL 1389
            FVTNLILAVVYDSFKS+L KQV+E D+ R  ILKKAFNL+D  ++G LNKDQCI+LFEEL
Sbjct: 296  FVTNLILAVVYDSFKSQLAKQVSEMDQSRKSILKKAFNLLDKDNVGSLNKDQCIQLFEEL 355

Query: 1388 NKYRTLPKISRXXXXXXXXXXXDSHDFKINLDEFADLVNAIALRFQKEDSLPIFESCPTF 1209
            NKYRTLPKISR            S DF+INL+EF DL NAI LRF KED+  +FE  P+F
Sbjct: 356  NKYRTLPKISREEFGLIFDELDASGDFQINLEEFTDLCNAIGLRFPKEDTPSLFEKFPSF 415

Query: 1208 YHSPASEKLKDFVRSPKFGYIIVFILILNFVAVVIETTLDIENNSAQEAWQKVEFVFGWL 1029
            YHS  SEKLK FVRSPKFGYII FILI+N VAV++ETTLDIENN+ Q+AWQ  EFVFGW+
Sbjct: 416  YHSLLSEKLKAFVRSPKFGYIISFILIVNLVAVIVETTLDIENNTGQKAWQAAEFVFGWI 475

Query: 1028 YVLEMALKVYAYGFENYWRDSQNRFDFIVTWVIVIGETTTFVSPHGLTFLSNGEWXXXXX 849
            YVLEMALKVY++GF+NYWRD QNRFDF+VTW+IVIGET TFV+P GL F SNGEW     
Sbjct: 476  YVLEMALKVYSFGFQNYWRDGQNRFDFLVTWIIVIGETITFVTPDGLIFFSNGEWIRYLL 535

Query: 848  XXXXXXXXXXXXRVERYRAFVTTFLTLIPSLMPYLGIIFCVLCIYCSLGVQIFGGIVNAG 669
                         V+RYRAF+ TFLTLIPSLMPYLG IFCV+CIYC+LGVQ+FGGIVNAG
Sbjct: 536  IARMLRLIRILMHVQRYRAFLATFLTLIPSLMPYLGTIFCVMCIYCTLGVQVFGGIVNAG 595

Query: 668  NPNLVGTDLSDDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKDLTGTTWSYVYFVS 489
            N +L GTD  ++DYLLFNFNDYPNGMVTLFNLLVMGNWQ W++ YK+LTGT WS  YFVS
Sbjct: 596  NASLEGTDFYENDYLLFNFNDYPNGMVTLFNLLVMGNWQIWIEGYKELTGTWWSMAYFVS 655

Query: 488  FYLISXXXXXXXXXXXXXXXXXXXXXXETSEKSEDGVGKEERGKDRRRNVGTKSRSQRVD 309
            FYLI+                      ETSEK E    + E  +DR R++G+K++SQR+D
Sbjct: 656  FYLITILLLLNLVVAFVLEAFFAEMDLETSEKCEGDEKEVEGAEDRHRSLGSKTQSQRID 715

Query: 308  ILLHHMLSAEL 276
            +LLHHMLSAEL
Sbjct: 716  VLLHHMLSAEL 726



 Score =  251 bits (641), Expect(2) = 0.0
 Identities = 122/222 (54%), Positives = 155/222 (69%), Gaps = 4/222 (1%)
 Frame = -3

Query: 2404 DRKHIFNRRSDAIAYGSTYQKAAALVDLAADGIGIPEEILDESSFGSSLKSYFVFIQFDF 2225
            DR  +F RRSDAI +GS YQKAAALVDLA DG+G+PE+ILD+ +F S+ K YF++++F F
Sbjct: 14   DRLAVFRRRSDAITFGSPYQKAAALVDLAEDGVGLPEQILDQENFQSAAKFYFLYVRFSF 73

Query: 2224 IWSLNYFALIVLNFLEKPLWCSE----NCSNRVYYFLGELPYLTGTESLIYEGITLLVLI 2057
            +W+  YFALI+LNFLEKPLWC+      CS+R YY+LGELPYL   ESLI+EGITL++L+
Sbjct: 74   LWAFLYFALIILNFLEKPLWCARYTEYTCSDRDYYYLGELPYLNAVESLIFEGITLIILM 133

Query: 2056 IHIFFPITYEGFGIYWKSXXXXXXXXXXXXXXXXXXXXXXXLSPVAFYTLPFRIAPYIRV 1877
            +H FFPI+YEGF +YWK+                       LSP AF +LP RIAPYIRV
Sbjct: 134  VHTFFPISYEGFNLYWKNHLNRFKVMGLLLLVADLMVYVLYLSPTAFDSLPLRIAPYIRV 193

Query: 1876 VLFILNIRELRESTVVLAGMLSTYSNVLGLCGFYFFYSLAGW 1751
            + FILN RELR   ++LAGML TY N+L +  +  F   A W
Sbjct: 194  IFFILNFRELRSCMLILAGMLGTYLNILAM--WLLFLLFASW 233


>ref|XP_004303095.1| PREDICTED: two pore calcium channel protein 1 isoform X1 [Fragaria
            vesca subsp. vesca]
          Length = 737

 Score =  691 bits (1784), Expect(2) = 0.0
 Identities = 341/493 (69%), Positives = 392/493 (79%)
 Frame = -2

Query: 1748 FVIFEDTEQGRTLFTSYGTTLYQMFVLFTTSNNPDVWIPAYKSSRWYCLFFILYVLLGVY 1569
            +V+FEDT+QG  +FTSYGTTLYQMFVLFTTSNNPDVWIPAYK+SRWYCLFF+LYVLLGVY
Sbjct: 239  YVMFEDTDQGTIVFTSYGTTLYQMFVLFTTSNNPDVWIPAYKASRWYCLFFVLYVLLGVY 298

Query: 1568 FVTNLILAVVYDSFKSELVKQVAEKDRVRMRILKKAFNLIDNYDLGYLNKDQCIRLFEEL 1389
            FVTNLILAVVYDSFKS+LVKQV+E D+ R  IL+KAF+LI+  + GY+NK+QCIRLFEEL
Sbjct: 299  FVTNLILAVVYDSFKSQLVKQVSEMDQTRKTILEKAFHLIEKDNSGYINKEQCIRLFEEL 358

Query: 1388 NKYRTLPKISRXXXXXXXXXXXDSHDFKINLDEFADLVNAIALRFQKEDSLPIFESCPTF 1209
            NKYRTLPK+SR           DS DFKIN+ EF DL NAIALRFQKED+   FE  P+F
Sbjct: 359  NKYRTLPKMSREEFELIFDELDDSGDFKINVQEFTDLCNAIALRFQKEDTPSWFEKFPSF 418

Query: 1208 YHSPASEKLKDFVRSPKFGYIIVFILILNFVAVVIETTLDIENNSAQEAWQKVEFVFGWL 1029
            YHSP SEKLK F+RSPKFGYII FILI+N  AV++ETTLDIENNSAQ+ WQ +EFV GW+
Sbjct: 419  YHSPVSEKLKAFIRSPKFGYIISFILIINLFAVIVETTLDIENNSAQKVWQVMEFVLGWV 478

Query: 1028 YVLEMALKVYAYGFENYWRDSQNRFDFIVTWVIVIGETTTFVSPHGLTFLSNGEWXXXXX 849
            YVLEMALKVY++GFENYWRD QNRFDF+VT +IVIGET T+ +P GLTFLSNGEW     
Sbjct: 479  YVLEMALKVYSFGFENYWRDGQNRFDFLVTLIIVIGETLTYAAPQGLTFLSNGEWIRYLL 538

Query: 848  XXXXXXXXXXXXRVERYRAFVTTFLTLIPSLMPYLGIIFCVLCIYCSLGVQIFGGIVNAG 669
                         V+R RAF+ TFLTL+PSLMPYLG IFCV+CIYC+LGVQIFGG+VNAG
Sbjct: 539  IARMLRLIRLLIHVQRCRAFIATFLTLLPSLMPYLGTIFCVMCIYCTLGVQIFGGMVNAG 598

Query: 668  NPNLVGTDLSDDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKDLTGTTWSYVYFVS 489
            N +L  TDL DDDYLLFNFNDYPNGMVTLFNLLV GNWQ WMQSYKDLTGT+WS VYFVS
Sbjct: 599  NASLEATDLYDDDYLLFNFNDYPNGMVTLFNLLVTGNWQVWMQSYKDLTGTSWSLVYFVS 658

Query: 488  FYLISXXXXXXXXXXXXXXXXXXXXXXETSEKSEDGVGKEERGKDRRRNVGTKSRSQRVD 309
            FYLI+                      E++   E+  G+ +  KDRRR+VG+KSRSQRVD
Sbjct: 659  FYLITVLLLLNLVVAFVLEAFFAEMDLESAGICEEHEGEVDGVKDRRRSVGSKSRSQRVD 718

Query: 308  ILLHHMLSAELDK 270
             LLHHMLS+EL++
Sbjct: 719  ALLHHMLSSELNQ 731



 Score =  246 bits (628), Expect(2) = 0.0
 Identities = 125/224 (55%), Positives = 156/224 (69%), Gaps = 4/224 (1%)
 Frame = -3

Query: 2410 RRDRKHIFNRRSDAIAYGSTYQKAAALVDLAADGIGIPEEILDESSFGSSLKSYFVFIQF 2231
            RR     F RRSDAI +GS++QKAAALVDLA DGIG+PE+ILD SSF S+ K YF++++F
Sbjct: 15   RRGGDKGFKRRSDAITFGSSFQKAAALVDLAEDGIGLPEQILDLSSFHSAAKYYFIYMRF 74

Query: 2230 DFIWSLNYFALIVLNFLEKPLWCSE----NCSNRVYYFLGELPYLTGTESLIYEGITLLV 2063
             F+W+L YFAL++LNFLE+PLWC+      CS+R YY+LG+LPYLT TESLIYEGITL+V
Sbjct: 75   SFLWALLYFALLILNFLERPLWCANLTDYTCSDRDYYYLGQLPYLTSTESLIYEGITLIV 134

Query: 2062 LIIHIFFPITYEGFGIYWKSXXXXXXXXXXXXXXXXXXXXXXXLSPVAFYTLPFRIAPYI 1883
            L++H FFPI+Y GF +YWKS                       LSPVAF +LP R+APYI
Sbjct: 135  LMVHTFFPISYVGFHLYWKSHLTKFKIICLLILVADLLAYVLYLSPVAFESLPLRVAPYI 194

Query: 1882 RVVLFILNIRELRESTVVLAGMLSTYSNVLGLCGFYFFYSLAGW 1751
            RV+ FIL+ RELR   ++LAGM  T+ NVL L     F   A W
Sbjct: 195  RVIFFILSFRELRSCMLILAGMFGTFLNVLAL--GLLFLLFASW 236


>ref|XP_010656956.1| PREDICTED: two pore channel 1 isoform X1 [Vitis vinifera]
            gi|302143292|emb|CBI21853.3| unnamed protein product
            [Vitis vinifera]
          Length = 732

 Score =  675 bits (1741), Expect(2) = 0.0
 Identities = 337/494 (68%), Positives = 386/494 (78%), Gaps = 1/494 (0%)
 Frame = -2

Query: 1748 FVIFEDTEQGRTLFTSYGTTLYQMFVLFTTSNNPDVWIPAYKSSRWYCLFFILYVLLGVY 1569
            +V+FEDT+QG+T+FTSY TTLYQMF+LFTTSNNPDVWIPAYK SRWYCLFF+LY+LLGVY
Sbjct: 239  YVMFEDTQQGKTVFTSYLTTLYQMFILFTTSNNPDVWIPAYKVSRWYCLFFVLYMLLGVY 298

Query: 1568 FVTNLILAVVYDSFKSELVKQVAEKDRVRMRILKKAFNLIDNYDLGYLNKDQCIRLFEEL 1389
            F+TNLILAV+YDSFK++L KQVAE DR+R RIL+KAFNLID  +LG+L K+QCI LFEEL
Sbjct: 299  FMTNLILAVIYDSFKNQLAKQVAEMDRMRRRILEKAFNLIDKSNLGFLKKEQCIHLFEEL 358

Query: 1388 NKYRTLPKISRXXXXXXXXXXXDSHDFKINLDEFADLVNAIALRFQKEDSLPIFESCPTF 1209
            NKYR+LPKISR           DS+DFKINLDEF DL NAI L FQKEDS   FE  P+F
Sbjct: 359  NKYRSLPKISREDFELIFDELDDSNDFKINLDEFFDLCNAITLSFQKEDSQSWFERYPSF 418

Query: 1208 YHSPASEKLKDFVRSPKFGYIIVFILILNFVAVVIETTLDIENNSAQEAWQKVEFVFGWL 1029
            YHSPA EKLK FVRS  FG +I  IL+LN VAV+IETTLDIENNSAQ+ WQKVEFV GW+
Sbjct: 419  YHSPACEKLKAFVRSSIFGNMIAVILVLNLVAVIIETTLDIENNSAQKVWQKVEFVLGWI 478

Query: 1028 YVLEMALKVYAYGFENYWRDSQNRFDFIVTWVIVIGETTTFVSPHGLTFLSNGEWXXXXX 849
            YVLEMALKV+A+GF+NYWRD  NRFDF +TW+IVIGET TFV+P  L+FLSNGEW     
Sbjct: 479  YVLEMALKVFAFGFKNYWRDDSNRFDFFITWIIVIGETATFVAPSSLSFLSNGEWIRYLL 538

Query: 848  XXXXXXXXXXXXRVERYRAFVTTFLTLIPSLMPYLGIIFCVLCIYCSLGVQIFGGIVNAG 669
                        RV++YRAF+ TFL LIPSLMPYLG IFCVLCIYCSLGVQIFGGIVN G
Sbjct: 539  LARMLRLIRLLMRVKQYRAFIATFLNLIPSLMPYLGTIFCVLCIYCSLGVQIFGGIVNNG 598

Query: 668  NPNLVGTDLSDDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKDLTGTTWSYVYFVS 489
            N NL GTDL + DYLLFNFNDYPNGMVTLFNLLVM NWQ WMQSYKDLTGT+W+ VYF+S
Sbjct: 599  NSNLEGTDLEESDYLLFNFNDYPNGMVTLFNLLVMNNWQVWMQSYKDLTGTSWTLVYFIS 658

Query: 488  FYLISXXXXXXXXXXXXXXXXXXXXXXETSEK-SEDGVGKEERGKDRRRNVGTKSRSQRV 312
            +YL++                      E SE   EDG   +E G+ +RR   ++ RSQRV
Sbjct: 659  YYLLTVLLLLNLVVAFVLEAFFAEMDLEKSENCGEDG---KEAGEKQRRYTRSRIRSQRV 715

Query: 311  DILLHHMLSAELDK 270
            DILLHHMLSAEL++
Sbjct: 716  DILLHHMLSAELNQ 729



 Score =  262 bits (669), Expect(2) = 0.0
 Identities = 131/224 (58%), Positives = 164/224 (73%), Gaps = 4/224 (1%)
 Frame = -3

Query: 2410 RRDRKHIFNRRSDAIAYGSTYQKAAALVDLAADGIGIPEEILDESSFGSSLKSYFVFIQF 2231
            RR +  +F+RRSDAIAYGS YQKAAALVDLA DGIG+PE++LD+ SF S+ K YF+FI+F
Sbjct: 15   RRGQTPVFHRRSDAIAYGSPYQKAAALVDLAEDGIGLPEQMLDQESFESAAKFYFIFIRF 74

Query: 2230 DFIWSLNYFALIVLNFLEKPLWCSE----NCSNRVYYFLGELPYLTGTESLIYEGITLLV 2063
            +F+WSLN FALIVLNFLEKPLWC++    +C++R YYFLG+LPYLT  ESLIYEGITL++
Sbjct: 75   NFLWSLNLFALIVLNFLEKPLWCAKQTTYSCNDREYYFLGQLPYLTSVESLIYEGITLVI 134

Query: 2062 LIIHIFFPITYEGFGIYWKSXXXXXXXXXXXXXXXXXXXXXXXLSPVAFYTLPFRIAPYI 1883
            +++H FFPI+YEG  IYWK+                       LSP+ FY LPFR+APYI
Sbjct: 135  ILLHTFFPISYEGSQIYWKNPLNRLKVILLSILVADLLVYVLYLSPLPFYYLPFRLAPYI 194

Query: 1882 RVVLFILNIRELRESTVVLAGMLSTYSNVLGLCGFYFFYSLAGW 1751
            RV+L ILNIRE+R + V+LAGML TY N++ L     F   A W
Sbjct: 195  RVILCILNIREVRATMVILAGMLGTYLNIMAL--GLLFLLFASW 236


>ref|XP_010035755.1| PREDICTED: two pore calcium channel protein 1-like [Eucalyptus
            grandis]
          Length = 736

 Score =  690 bits (1780), Expect(2) = 0.0
 Identities = 340/493 (68%), Positives = 389/493 (78%)
 Frame = -2

Query: 1748 FVIFEDTEQGRTLFTSYGTTLYQMFVLFTTSNNPDVWIPAYKSSRWYCLFFILYVLLGVY 1569
            +V+FEDT QG+T FTSYG T Y+M VLFTTSNNPDVWIPAYK SRWY LFF+LY+LLGVY
Sbjct: 238  YVMFEDTAQGKTEFTSYGATFYKMLVLFTTSNNPDVWIPAYKVSRWYSLFFVLYILLGVY 297

Query: 1568 FVTNLILAVVYDSFKSELVKQVAEKDRVRMRILKKAFNLIDNYDLGYLNKDQCIRLFEEL 1389
            FVTNL+LAVVYDSFKS+LVKQV+E DR+R  +L+KAFNLID   +GY+NK+QC+ LFEEL
Sbjct: 298  FVTNLVLAVVYDSFKSQLVKQVSEMDRMREYMLRKAFNLIDKNKVGYINKEQCVHLFEEL 357

Query: 1388 NKYRTLPKISRXXXXXXXXXXXDSHDFKINLDEFADLVNAIALRFQKEDSLPIFESCPTF 1209
            N YRTLPKISR           DSHDFKIN+DEFADL NAIALRFQKED   +FE CP+ 
Sbjct: 358  NHYRTLPKISREDFELIFVELDDSHDFKINMDEFADLCNAIALRFQKEDVPSLFEKCPSI 417

Query: 1208 YHSPASEKLKDFVRSPKFGYIIVFILILNFVAVVIETTLDIENNSAQEAWQKVEFVFGWL 1029
            Y+SP SEKLK FV+SPKFGY+I F LILN VAV++ETTLDIENNSAQ+ WQ++EF+FGW+
Sbjct: 418  YYSPFSEKLKGFVKSPKFGYMISFFLILNLVAVIVETTLDIENNSAQKVWQEIEFIFGWV 477

Query: 1028 YVLEMALKVYAYGFENYWRDSQNRFDFIVTWVIVIGETTTFVSPHGLTFLSNGEWXXXXX 849
            YVLEMALKVY+ GFENYWRD QNRFDF++TWVIVIGET TFV+P+GL FL NGEW     
Sbjct: 478  YVLEMALKVYSLGFENYWRDGQNRFDFVITWVIVIGETITFVAPNGLDFLLNGEWIRYLL 537

Query: 848  XXXXXXXXXXXXRVERYRAFVTTFLTLIPSLMPYLGIIFCVLCIYCSLGVQIFGGIVNAG 669
                         V+RYRAFV TFLTL+PSLMPYLGIIFCVLCIYCSL  QIFGG+VNAG
Sbjct: 538  LARLLRLMRLLMHVKRYRAFVATFLTLLPSLMPYLGIIFCVLCIYCSLAEQIFGGMVNAG 597

Query: 668  NPNLVGTDLSDDDYLLFNFNDYPNGMVTLFNLLVMGNWQAWMQSYKDLTGTTWSYVYFVS 489
            N  L  TDL  DDYLLFNFNDYPNGMVTLFNLLVM NWQ WMQSYK+LTGT+W+ +YF+S
Sbjct: 598  NSKLEETDLMADDYLLFNFNDYPNGMVTLFNLLVMNNWQDWMQSYKELTGTSWTLIYFIS 657

Query: 488  FYLISXXXXXXXXXXXXXXXXXXXXXXETSEKSEDGVGKEERGKDRRRNVGTKSRSQRVD 309
            FYLI+                      E+SE  E+   KE RG+ R  +VGTK+RSQRVD
Sbjct: 658  FYLITVLLLLNLVVAFVLEAFFTEMDLESSENCEE-QDKETRGR-RPHSVGTKTRSQRVD 715

Query: 308  ILLHHMLSAELDK 270
            +LLHHMLSAELDK
Sbjct: 716  VLLHHMLSAELDK 728



 Score =  246 bits (627), Expect(2) = 0.0
 Identities = 127/220 (57%), Positives = 154/220 (70%), Gaps = 5/220 (2%)
 Frame = -3

Query: 2407 RDRKHIFNRRSDAIAYGSTYQKAAALVDLAADGIGIPEEILDESSFGSSLKSYFVFIQFD 2228
            R RK  F RRSDA+AYGS YQKAA LVDLA DG+G+P +ILD+S+F S+ K YFVF +FD
Sbjct: 14   RRRKERFKRRSDALAYGSPYQKAATLVDLAEDGVGLPVQILDQSNFESAAKFYFVFTKFD 73

Query: 2227 FIWSLNYFALIVLNFLEKPLWC----SENCSNRVYYFLGELPYLTGTESLIYEGITLLVL 2060
             IWSLNYFALIVLNF EKPLWC    ++ CS R Y+FLG+LPYLTG ESLIYE +TL++L
Sbjct: 74   IIWSLNYFALIVLNFFEKPLWCAKYSADACSEREYFFLGQLPYLTGVESLIYESVTLVIL 133

Query: 2059 IIHIFFPITYEGFGIYWKSXXXXXXXXXXXXXXXXXXXXXXXLSP-VAFYTLPFRIAPYI 1883
             IH FFPI+YEGF +Y ++                       LSP VA   LPFR APY+
Sbjct: 134  TIHTFFPISYEGFRLYSRNHLNQLKVVCLLLLVADLIAYALHLSPAVALDYLPFRAAPYV 193

Query: 1882 RVVLFILNIRELRESTVVLAGMLSTYSNVLGLCGFYFFYS 1763
            RV+ FI++IRELR +T +LAGML TY NVL L   +  +S
Sbjct: 194  RVIFFIMSIRELRATTSILAGMLGTYLNVLALLLLFLLFS 233