BLASTX nr result
ID: Forsythia21_contig00048699
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Forsythia21_contig00048699
(315 letters)
Database: ./nr
69,698,275 sequences; 24,982,196,650 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
gb|EKG13864.1| Catalase [Macrophomina phaseolina MS6] 189 5e-46
gb|KIW08966.1| catalase-1 [Verruconis gallopava] 189 8e-46
gb|KKY24704.1| putative catalase a [Diplodia seriata] 184 2e-44
gb|KJX96041.1| catalase like protein [Zymoseptoria brevis] 179 8e-43
ref|XP_007784339.1| catalase-1 [Coniosporium apollinis CBS 10021... 179 8e-43
gb|AAG53519.1|AF222056_1 CAT2 catalase [Passalora fulva] 177 2e-42
ref|XP_003857549.1| hypothetical protein MYCGRDRAFT_98331 [Zymos... 176 5e-42
ref|XP_007673478.1| hypothetical protein BAUCODRAFT_30922 [Baudo... 175 1e-41
gb|EME46076.1| hypothetical protein DOTSEDRAFT_70163 [Dothistrom... 173 3e-41
gb|KEQ79411.1| catalase [Aureobasidium pullulans EXF-150] 172 6e-41
gb|KEQ77600.1| catalase [Aureobasidium namibiae CBS 147.97] 171 2e-40
gb|KEQ67282.1| CAT2 catalase [Aureobasidium melanogenum CBS 110374] 169 5e-40
ref|XP_001804330.1| hypothetical protein SNOG_14133 [Phaeosphaer... 169 7e-40
gb|EMF14529.1| CAT2 catalase, partial [Sphaerulina musiva SO2202] 167 2e-39
ref|XP_001935077.1| catalase A [Pyrenophora tritici-repentis Pt-... 166 7e-39
gb|KJZ76509.1| Catalase-1 [Hirsutella minnesotensis 3608] 165 1e-38
ref|XP_003841429.1| similar to catalase [Leptosphaeria maculans ... 165 1e-38
ref|XP_007687460.1| hypothetical protein COCMIDRAFT_25885 [Bipol... 164 2e-38
ref|XP_003305071.1| hypothetical protein PTT_17818 [Pyrenophora ... 164 2e-38
ref|XP_007717439.1| hypothetical protein COCCADRAFT_9336 [Bipola... 163 4e-38
>gb|EKG13864.1| Catalase [Macrophomina phaseolina MS6]
Length = 736
Score = 189 bits (481), Expect = 5e-46
Identities = 89/104 (85%), Positives = 99/104 (95%)
Frame = -2
Query: 314 TRRVAIIIADGYDPVAYNGIKAALTAAKALPFTIAPRRTPIFAAGEDKTTGKGVTPDHHL 135
TRRVAIIIADGYDPVAY GIK AL AA+ALPFTIAPRRTPIFAAGED+ T KGVTPDHHL
Sbjct: 550 TRRVAIIIADGYDPVAYTGIKTALKAAQALPFTIAPRRTPIFAAGEDRKTSKGVTPDHHL 609
Query: 134 EGMRSTMFDSVFVPGGADSISTLRKNGRSVHWIREAFGHLKAIG 3
EGMRSTMFDS+F+PGGA+SI+TL+K+GR++HWIREAFGHLKAIG
Sbjct: 610 EGMRSTMFDSIFIPGGAESINTLKKSGRALHWIREAFGHLKAIG 653
>gb|KIW08966.1| catalase-1 [Verruconis gallopava]
Length = 759
Score = 189 bits (479), Expect = 8e-46
Identities = 87/104 (83%), Positives = 99/104 (95%)
Frame = -2
Query: 314 TRRVAIIIADGYDPVAYNGIKAALTAAKALPFTIAPRRTPIFAAGEDKTTGKGVTPDHHL 135
+RRVAIIIADGYDP+AY+GIKAAL AA+ALPFTIAPRRTPIFAAGED++ GKGV PDHHL
Sbjct: 573 SRRVAIIIADGYDPIAYHGIKAALKAAQALPFTIAPRRTPIFAAGEDRSNGKGVVPDHHL 632
Query: 134 EGMRSTMFDSVFVPGGADSISTLRKNGRSVHWIREAFGHLKAIG 3
EGMRSTMFDSVF+PGG SI+TL+KNGR++HW+REAFGHLKAIG
Sbjct: 633 EGMRSTMFDSVFIPGGEQSIATLKKNGRAIHWVREAFGHLKAIG 676
>gb|KKY24704.1| putative catalase a [Diplodia seriata]
Length = 739
Score = 184 bits (468), Expect = 2e-44
Identities = 87/104 (83%), Positives = 97/104 (93%)
Frame = -2
Query: 314 TRRVAIIIADGYDPVAYNGIKAALTAAKALPFTIAPRRTPIFAAGEDKTTGKGVTPDHHL 135
+RRVAIIIADG+DP AY GIKAALTAAKA PFTI RR+PIFAAGED+ TGKGV PDHHL
Sbjct: 553 SRRVAIIIADGFDPAAYAGIKAALTAAKAFPFTIGTRRSPIFAAGEDRKTGKGVKPDHHL 612
Query: 134 EGMRSTMFDSVFVPGGADSISTLRKNGRSVHWIREAFGHLKAIG 3
EGMRSTMFDSVF+PGGADSI+TLRK+GR++HW+REAFGHLKAIG
Sbjct: 613 EGMRSTMFDSVFIPGGADSIATLRKSGRALHWVREAFGHLKAIG 656
>gb|KJX96041.1| catalase like protein [Zymoseptoria brevis]
Length = 746
Score = 179 bits (453), Expect = 8e-43
Identities = 84/104 (80%), Positives = 94/104 (90%)
Frame = -2
Query: 314 TRRVAIIIADGYDPVAYNGIKAALTAAKALPFTIAPRRTPIFAAGEDKTTGKGVTPDHHL 135
TRRVAIIIADG+D AYNGI AAL A KALPFTIAPRR+PIFAAGE K +GKG PDHHL
Sbjct: 560 TRRVAIIIADGFDSTAYNGIVAALKAQKALPFTIAPRRSPIFAAGESKGSGKGTQPDHHL 619
Query: 134 EGMRSTMFDSVFVPGGADSISTLRKNGRSVHWIREAFGHLKAIG 3
EGMRSTMFD++FVPGGADS++TLRKNGR++HW+REAF HLKAIG
Sbjct: 620 EGMRSTMFDAIFVPGGADSVATLRKNGRALHWVREAFAHLKAIG 663
>ref|XP_007784339.1| catalase-1 [Coniosporium apollinis CBS 100218]
gi|494832889|gb|EON69022.1| catalase-1 [Coniosporium
apollinis CBS 100218]
Length = 745
Score = 179 bits (453), Expect = 8e-43
Identities = 84/104 (80%), Positives = 96/104 (92%)
Frame = -2
Query: 314 TRRVAIIIADGYDPVAYNGIKAALTAAKALPFTIAPRRTPIFAAGEDKTTGKGVTPDHHL 135
TRRVAII+ DGYDPVAYNG+KAALTAA+ALPFTI +R+ I+AAGEDK+TGKGV PDHHL
Sbjct: 560 TRRVAIIVGDGYDPVAYNGVKAALTAAQALPFTIGTKRSTIYAAGEDKSTGKGVKPDHHL 619
Query: 134 EGMRSTMFDSVFVPGGADSISTLRKNGRSVHWIREAFGHLKAIG 3
EGMRSTMFDSVF+PGGA + TLRKNGR++HW+REAFGHLKAIG
Sbjct: 620 EGMRSTMFDSVFIPGGA-HVETLRKNGRALHWVREAFGHLKAIG 662
>gb|AAG53519.1|AF222056_1 CAT2 catalase [Passalora fulva]
Length = 745
Score = 177 bits (449), Expect = 2e-42
Identities = 84/104 (80%), Positives = 93/104 (89%)
Frame = -2
Query: 314 TRRVAIIIADGYDPVAYNGIKAALTAAKALPFTIAPRRTPIFAAGEDKTTGKGVTPDHHL 135
TRR+AIIIADGYD VAYNGI AA A +ALPFTIAPRR+PI+AAGE K KGV PDHHL
Sbjct: 559 TRRIAIIIADGYDLVAYNGIVAAAKACQALPFTIAPRRSPIYAAGESKENSKGVKPDHHL 618
Query: 134 EGMRSTMFDSVFVPGGADSISTLRKNGRSVHWIREAFGHLKAIG 3
EGMRSTMFDSVFVPGGADS++TLRKNGR++HW+REAF HLKAIG
Sbjct: 619 EGMRSTMFDSVFVPGGADSVATLRKNGRALHWVREAFAHLKAIG 662
>ref|XP_003857549.1| hypothetical protein MYCGRDRAFT_98331 [Zymoseptoria tritici IPO323]
gi|339477434|gb|EGP92525.1| hypothetical protein
MYCGRDRAFT_98331 [Zymoseptoria tritici IPO323]
Length = 746
Score = 176 bits (446), Expect = 5e-42
Identities = 83/104 (79%), Positives = 93/104 (89%)
Frame = -2
Query: 314 TRRVAIIIADGYDPVAYNGIKAALTAAKALPFTIAPRRTPIFAAGEDKTTGKGVTPDHHL 135
TRRVAIIIADG+D AYNGI AAL A KALPFTIAPRR+PIFAAGE K +GKG PDHHL
Sbjct: 560 TRRVAIIIADGFDSTAYNGIVAALKAQKALPFTIAPRRSPIFAAGESKDSGKGTQPDHHL 619
Query: 134 EGMRSTMFDSVFVPGGADSISTLRKNGRSVHWIREAFGHLKAIG 3
EGMRSTMFD++ VPGGADS++TLRKNGR++HW+REAF HLKAIG
Sbjct: 620 EGMRSTMFDAICVPGGADSVATLRKNGRALHWVREAFAHLKAIG 663
>ref|XP_007673478.1| hypothetical protein BAUCODRAFT_30922 [Baudoinia compniacensis UAMH
10762] gi|449302644|gb|EMC98652.1| hypothetical protein
BAUCODRAFT_30922 [Baudoinia compniacensis UAMH 10762]
Length = 743
Score = 175 bits (443), Expect = 1e-41
Identities = 82/104 (78%), Positives = 93/104 (89%)
Frame = -2
Query: 314 TRRVAIIIADGYDPVAYNGIKAALTAAKALPFTIAPRRTPIFAAGEDKTTGKGVTPDHHL 135
TRRVAIIIADGYDPVAYNG+ AAL AA ALP+TI PRR+ ++AAGE K GKG PDHHL
Sbjct: 557 TRRVAIIIADGYDPVAYNGMVAALKAAGALPYTIGPRRSNVYAAGESKEAGKGKQPDHHL 616
Query: 134 EGMRSTMFDSVFVPGGADSISTLRKNGRSVHWIREAFGHLKAIG 3
EGMRSTMFDS+FVPGGADSI+TLRKNGR++HW+REA+ HLKAIG
Sbjct: 617 EGMRSTMFDSIFVPGGADSIATLRKNGRALHWVREAYAHLKAIG 660
>gb|EME46076.1| hypothetical protein DOTSEDRAFT_70163 [Dothistroma septosporum
NZE10]
Length = 752
Score = 173 bits (439), Expect = 3e-41
Identities = 81/104 (77%), Positives = 92/104 (88%)
Frame = -2
Query: 314 TRRVAIIIADGYDPVAYNGIKAALTAAKALPFTIAPRRTPIFAAGEDKTTGKGVTPDHHL 135
TRR+AI+IADGYD VAYNGI AA A +ALPFTIAPRR+PI+AAGE K +GV PDHHL
Sbjct: 566 TRRIAIVIADGYDKVAYNGIVAAAKACQALPFTIAPRRSPIYAAGETKDNSQGVKPDHHL 625
Query: 134 EGMRSTMFDSVFVPGGADSISTLRKNGRSVHWIREAFGHLKAIG 3
EGMRSTMFDSVFVPGGADS++TLRK GR++HW+REAF HLKAIG
Sbjct: 626 EGMRSTMFDSVFVPGGADSVATLRKTGRALHWVREAFAHLKAIG 669
>gb|KEQ79411.1| catalase [Aureobasidium pullulans EXF-150]
Length = 740
Score = 172 bits (437), Expect = 6e-41
Identities = 81/104 (77%), Positives = 94/104 (90%)
Frame = -2
Query: 314 TRRVAIIIADGYDPVAYNGIKAALTAAKALPFTIAPRRTPIFAAGEDKTTGKGVTPDHHL 135
TR VAI+IADGYD VAYN IKAAL A ALPFTIAPRRTPI+AAGE K +GKGV PDH+L
Sbjct: 554 TRMVAILIADGYDAVAYNAIKAALAAQGALPFTIAPRRTPIYAAGESKDSGKGVAPDHNL 613
Query: 134 EGMRSTMFDSVFVPGGADSISTLRKNGRSVHWIREAFGHLKAIG 3
EG RSTM+D++F+PGGADSI+TLRKNGR++H++REAFGHLKAIG
Sbjct: 614 EGQRSTMYDAIFIPGGADSIATLRKNGRALHYVREAFGHLKAIG 657
>gb|KEQ77600.1| catalase [Aureobasidium namibiae CBS 147.97]
Length = 736
Score = 171 bits (432), Expect = 2e-40
Identities = 80/104 (76%), Positives = 94/104 (90%)
Frame = -2
Query: 314 TRRVAIIIADGYDPVAYNGIKAALTAAKALPFTIAPRRTPIFAAGEDKTTGKGVTPDHHL 135
TR VAI+IADGYD VAYN IKAAL A ALPFTIAPRRTPI+AAGE K +GKG+ PDH+L
Sbjct: 550 TRMVAILIADGYDSVAYNAIKAALAAQGALPFTIAPRRTPIYAAGESKDSGKGLVPDHNL 609
Query: 134 EGMRSTMFDSVFVPGGADSISTLRKNGRSVHWIREAFGHLKAIG 3
EG RSTM+D++FVPGGADSI+TLRKNGR++H++REAFGHLK+IG
Sbjct: 610 EGQRSTMYDAIFVPGGADSIATLRKNGRALHYVREAFGHLKSIG 653
>gb|KEQ67282.1| CAT2 catalase [Aureobasidium melanogenum CBS 110374]
Length = 711
Score = 169 bits (429), Expect = 5e-40
Identities = 77/104 (74%), Positives = 94/104 (90%)
Frame = -2
Query: 314 TRRVAIIIADGYDPVAYNGIKAALTAAKALPFTIAPRRTPIFAAGEDKTTGKGVTPDHHL 135
TR VAI+IADGYD +AYN +KAAL A ALPFTIAPRRTPI+AAGE K +GKG+ PDH+L
Sbjct: 525 TRMVAILIADGYDSIAYNAVKAALAAQGALPFTIAPRRTPIYAAGESKESGKGLVPDHNL 584
Query: 134 EGMRSTMFDSVFVPGGADSISTLRKNGRSVHWIREAFGHLKAIG 3
EG RSTM+D++F+PGGADSI+TLRKNGR++H++REAFGHLK+IG
Sbjct: 585 EGQRSTMYDAIFIPGGADSIATLRKNGRALHYVREAFGHLKSIG 628
>ref|XP_001804330.1| hypothetical protein SNOG_14133 [Phaeosphaeria nodorum SN15]
gi|111057250|gb|EAT78370.1| hypothetical protein
SNOG_14133 [Phaeosphaeria nodorum SN15]
Length = 743
Score = 169 bits (428), Expect = 7e-40
Identities = 84/104 (80%), Positives = 91/104 (87%)
Frame = -2
Query: 314 TRRVAIIIADGYDPVAYNGIKAALTAAKALPFTIAPRRTPIFAAGEDKTTGKGVTPDHHL 135
TR VAIIIADGYD VAY GIKAALTAA ALPFTI+PRR IFA GEDK++G GV DHHL
Sbjct: 558 TRMVAIIIADGYDKVAYEGIKAALTAAGALPFTISPRRNNIFAEGEDKSSG-GVKADHHL 616
Query: 134 EGMRSTMFDSVFVPGGADSISTLRKNGRSVHWIREAFGHLKAIG 3
EGMRSTMFDSVFVPGGA S+ TL KNGR++HW+REAFGHLKAIG
Sbjct: 617 EGMRSTMFDSVFVPGGAKSVETLSKNGRAIHWVREAFGHLKAIG 660
>gb|EMF14529.1| CAT2 catalase, partial [Sphaerulina musiva SO2202]
Length = 746
Score = 167 bits (424), Expect = 2e-39
Identities = 80/104 (76%), Positives = 88/104 (84%)
Frame = -2
Query: 314 TRRVAIIIADGYDPVAYNGIKAALTAAKALPFTIAPRRTPIFAAGEDKTTGKGVTPDHHL 135
TRRVAIIIADG+D YNGI AL A ALPFTIAPRR+ I+AAGE K KG PDHHL
Sbjct: 560 TRRVAIIIADGFDKTVYNGIVTALKGAGALPFTIAPRRSVIYAAGETKDNSKGTQPDHHL 619
Query: 134 EGMRSTMFDSVFVPGGADSISTLRKNGRSVHWIREAFGHLKAIG 3
EGMRSTMFDS+F+PGGADS+STLRKNGR++HWIREAF HLKAIG
Sbjct: 620 EGMRSTMFDSIFLPGGADSVSTLRKNGRALHWIREAFAHLKAIG 663
>ref|XP_001935077.1| catalase A [Pyrenophora tritici-repentis Pt-1C-BFP]
gi|187981025|gb|EDU47651.1| catalase A [Pyrenophora
tritici-repentis Pt-1C-BFP]
Length = 740
Score = 166 bits (419), Expect = 7e-39
Identities = 82/104 (78%), Positives = 90/104 (86%)
Frame = -2
Query: 314 TRRVAIIIADGYDPVAYNGIKAALTAAKALPFTIAPRRTPIFAAGEDKTTGKGVTPDHHL 135
TR VAI+IADGYD VAYNGIKAALTAA ALPFTI+PRR I+A GED++ G GV DHHL
Sbjct: 555 TRMVAILIADGYDQVAYNGIKAALTAAGALPFTISPRRNKIYADGEDRS-GPGVVADHHL 613
Query: 134 EGMRSTMFDSVFVPGGADSISTLRKNGRSVHWIREAFGHLKAIG 3
EG RSTM+DSVFVPGGA SI TL KNGR+VHW+REAFGHLKAIG
Sbjct: 614 EGQRSTMYDSVFVPGGAKSIETLSKNGRAVHWVREAFGHLKAIG 657
>gb|KJZ76509.1| Catalase-1 [Hirsutella minnesotensis 3608]
Length = 737
Score = 165 bits (418), Expect = 1e-38
Identities = 75/104 (72%), Positives = 89/104 (85%)
Frame = -2
Query: 314 TRRVAIIIADGYDPVAYNGIKAALTAAKALPFTIAPRRTPIFAAGEDKTTGKGVTPDHHL 135
TRR+AIIIADGYDPVA+ ++ AL AAKA PF I PRR+ I+AAGED ++GKG+ PDHHL
Sbjct: 551 TRRIAIIIADGYDPVAFGAMQGALKAAKAFPFVIGPRRSAIYAAGEDSSSGKGIVPDHHL 610
Query: 134 EGMRSTMFDSVFVPGGADSISTLRKNGRSVHWIREAFGHLKAIG 3
EGMRSTMFD++ +PGG SISTL KNGR++HWIREAFGHLK IG
Sbjct: 611 EGMRSTMFDALLIPGGEKSISTLSKNGRAIHWIREAFGHLKPIG 654
>ref|XP_003841429.1| similar to catalase [Leptosphaeria maculans JN3]
gi|312218003|emb|CBX97950.1| similar to catalase
[Leptosphaeria maculans JN3]
Length = 743
Score = 165 bits (418), Expect = 1e-38
Identities = 83/104 (79%), Positives = 89/104 (85%)
Frame = -2
Query: 314 TRRVAIIIADGYDPVAYNGIKAALTAAKALPFTIAPRRTPIFAAGEDKTTGKGVTPDHHL 135
TR VAI+IADGYD VAYNGIKAALTAA ALPFTI+PRR IFAAGE+ G GV DHHL
Sbjct: 558 TRMVAILIADGYDKVAYNGIKAALTAAGALPFTISPRRNKIFAAGEEDGQG-GVVADHHL 616
Query: 134 EGMRSTMFDSVFVPGGADSISTLRKNGRSVHWIREAFGHLKAIG 3
EG RSTM+DS+FVPGGA SI TL KNGR+VHWIREAFGHLKAIG
Sbjct: 617 EGQRSTMYDSIFVPGGAKSIQTLSKNGRAVHWIREAFGHLKAIG 660
>ref|XP_007687460.1| hypothetical protein COCMIDRAFT_25885 [Bipolaris oryzae ATCC 44560]
gi|576932467|gb|EUC46008.1| hypothetical protein
COCMIDRAFT_25885 [Bipolaris oryzae ATCC 44560]
Length = 740
Score = 164 bits (416), Expect = 2e-38
Identities = 82/104 (78%), Positives = 88/104 (84%)
Frame = -2
Query: 314 TRRVAIIIADGYDPVAYNGIKAALTAAKALPFTIAPRRTPIFAAGEDKTTGKGVTPDHHL 135
TR VAI+IADGYD AY GIKAALTAA ALPFTI+PRR IFA GEDK+ G GV DHHL
Sbjct: 555 TRMVAILIADGYDKAAYTGIKAALTAAGALPFTISPRRNKIFADGEDKS-GSGVVADHHL 613
Query: 134 EGMRSTMFDSVFVPGGADSISTLRKNGRSVHWIREAFGHLKAIG 3
EG RSTM+DSVFVPGGA SI TL KNGR+VHW+REAFGHLKAIG
Sbjct: 614 EGQRSTMYDSVFVPGGAKSIETLSKNGRAVHWVREAFGHLKAIG 657
>ref|XP_003305071.1| hypothetical protein PTT_17818 [Pyrenophora teres f. teres 0-1]
gi|311318017|gb|EFQ86784.1| hypothetical protein
PTT_17818 [Pyrenophora teres f. teres 0-1]
Length = 740
Score = 164 bits (416), Expect = 2e-38
Identities = 81/104 (77%), Positives = 90/104 (86%)
Frame = -2
Query: 314 TRRVAIIIADGYDPVAYNGIKAALTAAKALPFTIAPRRTPIFAAGEDKTTGKGVTPDHHL 135
TR VAI+IADGYD VAYNG+KAALTAA ALPFTI+PRR I+A GED++ G GV DHHL
Sbjct: 555 TRMVAILIADGYDQVAYNGMKAALTAAGALPFTISPRRNKIYADGEDRS-GPGVVADHHL 613
Query: 134 EGMRSTMFDSVFVPGGADSISTLRKNGRSVHWIREAFGHLKAIG 3
EG RSTM+DSVFVPGGA SI TL KNGR+VHW+REAFGHLKAIG
Sbjct: 614 EGQRSTMYDSVFVPGGAKSIETLSKNGRAVHWVREAFGHLKAIG 657
>ref|XP_007717439.1| hypothetical protein COCCADRAFT_9336 [Bipolaris zeicola 26-R-13]
gi|576913855|gb|EUC28252.1| hypothetical protein
COCCADRAFT_9336 [Bipolaris zeicola 26-R-13]
gi|578485861|gb|EUN23348.1| hypothetical protein
COCVIDRAFT_109044 [Bipolaris victoriae FI3]
Length = 740
Score = 163 bits (413), Expect = 4e-38
Identities = 81/104 (77%), Positives = 88/104 (84%)
Frame = -2
Query: 314 TRRVAIIIADGYDPVAYNGIKAALTAAKALPFTIAPRRTPIFAAGEDKTTGKGVTPDHHL 135
TR VAI+IADGYD AY G+KAALTAA ALPFTI+PRR IFA GEDK+ G GV DHHL
Sbjct: 555 TRMVAILIADGYDKAAYTGMKAALTAAGALPFTISPRRNKIFADGEDKS-GSGVVADHHL 613
Query: 134 EGMRSTMFDSVFVPGGADSISTLRKNGRSVHWIREAFGHLKAIG 3
EG RSTM+DSVFVPGGA SI TL KNGR+VHW+REAFGHLKAIG
Sbjct: 614 EGQRSTMYDSVFVPGGAKSIETLSKNGRAVHWVREAFGHLKAIG 657