BLASTX nr result
ID: Forsythia21_contig00026271
seq
BLASTX 2.2.25 [Feb-01-2011] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Forsythia21_contig00026271 (526 letters) Database: ./nr 69,698,275 sequences; 24,982,196,650 total letters Searching..................................................done Score E Sequences producing significant alignments: (bits) Value ref|XP_011081058.1| PREDICTED: protein FAM63A-like [Sesamum indi... 55 7e-15 ref|XP_011071984.1| PREDICTED: protein FAM63B-like [Sesamum indi... 55 1e-14 ref|XP_002275737.1| PREDICTED: protein FAM63B [Vitis vinifera] 53 8e-14 emb|CBI34769.3| unnamed protein product [Vitis vinifera] 53 8e-14 ref|XP_012838039.1| PREDICTED: protein FAM63A [Erythranthe gutta... 52 1e-13 gb|EYU36933.1| hypothetical protein MIMGU_mgv1a022265mg, partial... 52 1e-13 ref|XP_007040084.1| Uncharacterized protein isoform 2 [Theobroma... 51 5e-13 ref|XP_007040083.1| Uncharacterized protein isoform 1 [Theobroma... 51 5e-13 ref|XP_006477357.1| PREDICTED: protein FAM63B-like [Citrus sinen... 51 5e-13 gb|KDO63619.1| hypothetical protein CISIN_1g036470mg [Citrus sin... 51 5e-13 ref|XP_007040087.1| Uncharacterized protein isoform 5 [Theobroma... 51 5e-13 ref|XP_009617781.1| PREDICTED: protein FAM63B [Nicotiana tomento... 50 6e-13 ref|XP_003611174.1| Protein FAM63A [Medicago truncatula] gi|3555... 52 8e-13 gb|KEH27444.1| FAM63A-like protein, putative [Medicago truncatula] 52 8e-13 ref|XP_012476035.1| PREDICTED: protein FAM63A-like isoform X1 [G... 51 1e-12 ref|XP_012476036.1| PREDICTED: protein FAM63A-like isoform X2 [G... 51 1e-12 ref|XP_006358850.1| PREDICTED: protein FAM63B-like [Solanum tube... 50 1e-12 ref|XP_009772020.1| PREDICTED: protein FAM63B [Nicotiana sylvest... 50 2e-12 gb|KHN34990.1| Protein FAM63A [Glycine soja] 50 3e-12 ref|XP_006440500.1| hypothetical protein CICLE_v10019075mg [Citr... 50 3e-12 >ref|XP_011081058.1| PREDICTED: protein FAM63A-like [Sesamum indicum] Length = 709 Score = 54.7 bits (130), Expect(2) = 7e-15 Identities = 31/62 (50%), Positives = 40/62 (64%), Gaps = 4/62 (6%) Frame = +3 Query: 3 DFSALLTLHADLYPSSCQILKNEDLETFSFS----EPIYEGEECILESRSTFYENQEPVY 170 D +LL AD S+ Q+ ++ E FS S EPIYEGEECIL+S + YE++EPVY Sbjct: 422 DSPSLLKAAADSNSSASQMHHIDEPEAFSSSVDGSEPIYEGEECILDSGTIAYESREPVY 481 Query: 171 EG 176 EG Sbjct: 482 EG 483 Score = 52.0 bits (123), Expect(2) = 7e-15 Identities = 27/43 (62%), Positives = 34/43 (79%), Gaps = 1/43 (2%) Frame = +1 Query: 193 EQVD-KSSIDDGDQTWKDIITSKQGELIRNFMRNSASQLTIYG 318 EQVD + +D G K+ I++KQGE+IRNF+RNSASQLTIYG Sbjct: 489 EQVDNRGCMDVGAPNSKERISTKQGEIIRNFLRNSASQLTIYG 531 >ref|XP_011071984.1| PREDICTED: protein FAM63B-like [Sesamum indicum] Length = 738 Score = 54.7 bits (130), Expect(2) = 1e-14 Identities = 28/42 (66%), Positives = 33/42 (78%) Frame = +1 Query: 193 EQVDKSSIDDGDQTWKDIITSKQGELIRNFMRNSASQLTIYG 318 EQVDK DDG +KD ++ KQGELIRNF+ NSASQLT+YG Sbjct: 510 EQVDKGCKDDG---FKDRLSVKQGELIRNFVNNSASQLTVYG 548 Score = 51.2 bits (121), Expect(2) = 1e-14 Identities = 31/62 (50%), Positives = 37/62 (59%), Gaps = 4/62 (6%) Frame = +3 Query: 3 DFSALLTLHADLYPSSCQILKNEDLETFSFS----EPIYEGEECILESRSTFYENQEPVY 170 D S LT ADL SS + K E F+ S EPIYEGEEC++ES + N+EPVY Sbjct: 443 DSSFSLTTTADLDSSSHPVHKIGMSEAFTSSIDDSEPIYEGEECVMESGNVVIGNREPVY 502 Query: 171 EG 176 EG Sbjct: 503 EG 504 >ref|XP_002275737.1| PREDICTED: protein FAM63B [Vitis vinifera] Length = 720 Score = 53.1 bits (126), Expect(2) = 8e-14 Identities = 23/42 (54%), Positives = 33/42 (78%) Frame = +1 Query: 193 EQVDKSSIDDGDQTWKDIITSKQGELIRNFMRNSASQLTIYG 318 EQ DK S+D + +KD +T QGEL+RNF++N+A+QLT+YG Sbjct: 508 EQADKDSVDSCNIGFKDELTPLQGELVRNFLKNNANQLTVYG 549 Score = 50.1 bits (118), Expect(2) = 8e-14 Identities = 20/27 (74%), Positives = 25/27 (92%) Frame = +3 Query: 96 EPIYEGEECILESRSTFYENQEPVYEG 176 EPIYEGEECIL+S +T YE++EP+YEG Sbjct: 476 EPIYEGEECILDSNTTIYEDREPMYEG 502 >emb|CBI34769.3| unnamed protein product [Vitis vinifera] Length = 662 Score = 53.1 bits (126), Expect(2) = 8e-14 Identities = 23/42 (54%), Positives = 33/42 (78%) Frame = +1 Query: 193 EQVDKSSIDDGDQTWKDIITSKQGELIRNFMRNSASQLTIYG 318 EQ DK S+D + +KD +T QGEL+RNF++N+A+QLT+YG Sbjct: 450 EQADKDSVDSCNIGFKDELTPLQGELVRNFLKNNANQLTVYG 491 Score = 50.1 bits (118), Expect(2) = 8e-14 Identities = 20/27 (74%), Positives = 25/27 (92%) Frame = +3 Query: 96 EPIYEGEECILESRSTFYENQEPVYEG 176 EPIYEGEECIL+S +T YE++EP+YEG Sbjct: 418 EPIYEGEECILDSNTTIYEDREPMYEG 444 >ref|XP_012838039.1| PREDICTED: protein FAM63A [Erythranthe guttatus] Length = 671 Score = 51.6 bits (122), Expect(2) = 1e-13 Identities = 31/62 (50%), Positives = 37/62 (59%), Gaps = 4/62 (6%) Frame = +3 Query: 3 DFSALLTLHADLYPSSCQILKNEDLETFSFS----EPIYEGEECILESRSTFYENQEPVY 170 D S+LLT ADL S Q+ K + +TF S EP+YEGEE I+ YEN EPVY Sbjct: 385 DTSSLLTTAADLDSSRGQVGKIDKPQTFPSSVDNTEPMYEGEERIMSPGPVVYENPEPVY 444 Query: 171 EG 176 EG Sbjct: 445 EG 446 Score = 51.2 bits (121), Expect(2) = 1e-13 Identities = 26/42 (61%), Positives = 32/42 (76%) Frame = +1 Query: 193 EQVDKSSIDDGDQTWKDIITSKQGELIRNFMRNSASQLTIYG 318 EQVD ++DGD + D I+ KQGE+IR FM+ SASQLTIYG Sbjct: 452 EQVDTGCVNDGD--FNDRISVKQGEVIREFMKTSASQLTIYG 491 >gb|EYU36933.1| hypothetical protein MIMGU_mgv1a022265mg, partial [Erythranthe guttata] Length = 644 Score = 51.6 bits (122), Expect(2) = 1e-13 Identities = 31/62 (50%), Positives = 37/62 (59%), Gaps = 4/62 (6%) Frame = +3 Query: 3 DFSALLTLHADLYPSSCQILKNEDLETFSFS----EPIYEGEECILESRSTFYENQEPVY 170 D S+LLT ADL S Q+ K + +TF S EP+YEGEE I+ YEN EPVY Sbjct: 385 DTSSLLTTAADLDSSRGQVGKIDKPQTFPSSVDNTEPMYEGEERIMSPGPVVYENPEPVY 444 Query: 171 EG 176 EG Sbjct: 445 EG 446 Score = 51.2 bits (121), Expect(2) = 1e-13 Identities = 26/42 (61%), Positives = 32/42 (76%) Frame = +1 Query: 193 EQVDKSSIDDGDQTWKDIITSKQGELIRNFMRNSASQLTIYG 318 EQVD ++DGD + D I+ KQGE+IR FM+ SASQLTIYG Sbjct: 452 EQVDTGCVNDGD--FNDRISVKQGEVIREFMKTSASQLTIYG 491 >ref|XP_007040084.1| Uncharacterized protein isoform 2 [Theobroma cacao] gi|508777329|gb|EOY24585.1| Uncharacterized protein isoform 2 [Theobroma cacao] Length = 752 Score = 51.2 bits (121), Expect(2) = 5e-13 Identities = 29/62 (46%), Positives = 41/62 (66%), Gaps = 4/62 (6%) Frame = +3 Query: 3 DFSALLTLHADLYPSSCQILKNEDLETFSFS----EPIYEGEECILESRSTFYENQEPVY 170 + S++ +AD SS +I + E F+ S EPIYEGEECIL+S +T YE++EP+Y Sbjct: 453 EVSSVSIQNADSDSSSGRIHHADVPEAFTSSLDGSEPIYEGEECILDSVTTTYEDREPIY 512 Query: 171 EG 176 EG Sbjct: 513 EG 514 Score = 49.3 bits (116), Expect(2) = 5e-13 Identities = 23/42 (54%), Positives = 31/42 (73%) Frame = +1 Query: 193 EQVDKSSIDDGDQTWKDIITSKQGELIRNFMRNSASQLTIYG 318 EQ DK +++ + KD IT +QGELI NF++N+ASQLT YG Sbjct: 520 EQADKKAVEGCNVRSKDEITPQQGELIANFLKNNASQLTFYG 561 >ref|XP_007040083.1| Uncharacterized protein isoform 1 [Theobroma cacao] gi|508777328|gb|EOY24584.1| Uncharacterized protein isoform 1 [Theobroma cacao] Length = 733 Score = 51.2 bits (121), Expect(2) = 5e-13 Identities = 29/62 (46%), Positives = 41/62 (66%), Gaps = 4/62 (6%) Frame = +3 Query: 3 DFSALLTLHADLYPSSCQILKNEDLETFSFS----EPIYEGEECILESRSTFYENQEPVY 170 + S++ +AD SS +I + E F+ S EPIYEGEECIL+S +T YE++EP+Y Sbjct: 453 EVSSVSIQNADSDSSSGRIHHADVPEAFTSSLDGSEPIYEGEECILDSVTTTYEDREPIY 512 Query: 171 EG 176 EG Sbjct: 513 EG 514 Score = 49.3 bits (116), Expect(2) = 5e-13 Identities = 23/42 (54%), Positives = 31/42 (73%) Frame = +1 Query: 193 EQVDKSSIDDGDQTWKDIITSKQGELIRNFMRNSASQLTIYG 318 EQ DK +++ + KD IT +QGELI NF++N+ASQLT YG Sbjct: 520 EQADKKAVEGCNVRSKDEITPQQGELIANFLKNNASQLTFYG 561 >ref|XP_006477357.1| PREDICTED: protein FAM63B-like [Citrus sinensis] Length = 709 Score = 50.8 bits (120), Expect(2) = 5e-13 Identities = 28/62 (45%), Positives = 39/62 (62%), Gaps = 4/62 (6%) Frame = +3 Query: 3 DFSALLTLHADLYPSSCQILKNEDLETFSFS----EPIYEGEECILESRSTFYENQEPVY 170 + S L + D SS ++ ++ E F+ S EPIYEGEECIL+S + YE++EPVY Sbjct: 428 EVSCLSVHNGDSDSSSGRVQLSDVPENFTSSVDGSEPIYEGEECILDSGTAMYEDREPVY 487 Query: 171 EG 176 EG Sbjct: 488 EG 489 Score = 49.7 bits (117), Expect(2) = 5e-13 Identities = 23/42 (54%), Positives = 33/42 (78%) Frame = +1 Query: 193 EQVDKSSIDDGDQTWKDIITSKQGELIRNFMRNSASQLTIYG 318 EQ DKS++D + KD IT +QGELI++F++N+A+QLT YG Sbjct: 495 EQADKSTLDACNVWSKDEITQQQGELIKSFLKNNANQLTFYG 536 >gb|KDO63619.1| hypothetical protein CISIN_1g036470mg [Citrus sinensis] Length = 697 Score = 50.8 bits (120), Expect(2) = 5e-13 Identities = 28/62 (45%), Positives = 39/62 (62%), Gaps = 4/62 (6%) Frame = +3 Query: 3 DFSALLTLHADLYPSSCQILKNEDLETFSFS----EPIYEGEECILESRSTFYENQEPVY 170 + S L + D SS ++ ++ E F+ S EPIYEGEECIL+S + YE++EPVY Sbjct: 416 EVSCLSVHNGDSDSSSGRVQLSDVPENFTSSVDGSEPIYEGEECILDSGTAMYEDREPVY 475 Query: 171 EG 176 EG Sbjct: 476 EG 477 Score = 49.7 bits (117), Expect(2) = 5e-13 Identities = 23/42 (54%), Positives = 33/42 (78%) Frame = +1 Query: 193 EQVDKSSIDDGDQTWKDIITSKQGELIRNFMRNSASQLTIYG 318 EQ DKS++D + KD IT +QGELI++F++N+A+QLT YG Sbjct: 483 EQADKSTLDACNVWSKDEITQQQGELIKSFLKNNANQLTFYG 524 >ref|XP_007040087.1| Uncharacterized protein isoform 5 [Theobroma cacao] gi|508777332|gb|EOY24588.1| Uncharacterized protein isoform 5 [Theobroma cacao] Length = 677 Score = 51.2 bits (121), Expect(2) = 5e-13 Identities = 29/62 (46%), Positives = 41/62 (66%), Gaps = 4/62 (6%) Frame = +3 Query: 3 DFSALLTLHADLYPSSCQILKNEDLETFSFS----EPIYEGEECILESRSTFYENQEPVY 170 + S++ +AD SS +I + E F+ S EPIYEGEECIL+S +T YE++EP+Y Sbjct: 453 EVSSVSIQNADSDSSSGRIHHADVPEAFTSSLDGSEPIYEGEECILDSVTTTYEDREPIY 512 Query: 171 EG 176 EG Sbjct: 513 EG 514 Score = 49.3 bits (116), Expect(2) = 5e-13 Identities = 23/42 (54%), Positives = 31/42 (73%) Frame = +1 Query: 193 EQVDKSSIDDGDQTWKDIITSKQGELIRNFMRNSASQLTIYG 318 EQ DK +++ + KD IT +QGELI NF++N+ASQLT YG Sbjct: 520 EQADKKAVEGCNVRSKDEITPQQGELIANFLKNNASQLTFYG 561 >ref|XP_009617781.1| PREDICTED: protein FAM63B [Nicotiana tomentosiformis] Length = 691 Score = 50.4 bits (119), Expect(2) = 6e-13 Identities = 24/42 (57%), Positives = 32/42 (76%) Frame = +1 Query: 193 EQVDKSSIDDGDQTWKDIITSKQGELIRNFMRNSASQLTIYG 318 EQV+ S D + KD IT ++GEL+RNF++NSASQLTI+G Sbjct: 476 EQVNGGSTDVPETIVKDEITQREGELVRNFLKNSASQLTIFG 517 Score = 49.7 bits (117), Expect(2) = 6e-13 Identities = 23/40 (57%), Positives = 32/40 (80%), Gaps = 4/40 (10%) Frame = +3 Query: 69 EDLETFSFS----EPIYEGEECILESRSTFYENQEPVYEG 176 ++ ETF+ S EPIYEGE+CILES +T Y+++EP+YEG Sbjct: 431 DEPETFNSSIDDDEPIYEGEDCILESATTSYQSREPMYEG 470 >ref|XP_003611174.1| Protein FAM63A [Medicago truncatula] gi|355512509|gb|AES94132.1| FAM63A-like protein, putative [Medicago truncatula] Length = 737 Score = 52.0 bits (123), Expect(2) = 8e-13 Identities = 24/42 (57%), Positives = 33/42 (78%) Frame = +1 Query: 193 EQVDKSSIDDGDQTWKDIITSKQGELIRNFMRNSASQLTIYG 318 EQ DKS++D D K+ IT +QGEL+++F+RN+ASQLT YG Sbjct: 524 EQADKSTLDALDPRAKEEITPEQGELVKSFLRNNASQLTFYG 565 Score = 47.8 bits (112), Expect(2) = 8e-13 Identities = 19/32 (59%), Positives = 27/32 (84%) Frame = +3 Query: 81 TFSFSEPIYEGEECILESRSTFYENQEPVYEG 176 T SEP+YEGEEC+L++++ +EN+EPVYEG Sbjct: 487 TVDGSEPMYEGEECVLDTKTGNFENREPVYEG 518 >gb|KEH27444.1| FAM63A-like protein, putative [Medicago truncatula] Length = 675 Score = 52.0 bits (123), Expect(2) = 8e-13 Identities = 24/42 (57%), Positives = 33/42 (78%) Frame = +1 Query: 193 EQVDKSSIDDGDQTWKDIITSKQGELIRNFMRNSASQLTIYG 318 EQ DKS++D D K+ IT +QGEL+++F+RN+ASQLT YG Sbjct: 524 EQADKSTLDALDPRAKEEITPEQGELVKSFLRNNASQLTFYG 565 Score = 47.8 bits (112), Expect(2) = 8e-13 Identities = 19/32 (59%), Positives = 27/32 (84%) Frame = +3 Query: 81 TFSFSEPIYEGEECILESRSTFYENQEPVYEG 176 T SEP+YEGEEC+L++++ +EN+EPVYEG Sbjct: 487 TVDGSEPMYEGEECVLDTKTGNFENREPVYEG 518 >ref|XP_012476035.1| PREDICTED: protein FAM63A-like isoform X1 [Gossypium raimondii] gi|763758400|gb|KJB25731.1| hypothetical protein B456_004G206500 [Gossypium raimondii] Length = 750 Score = 51.2 bits (121), Expect(2) = 1e-12 Identities = 23/42 (54%), Positives = 32/42 (76%) Frame = +1 Query: 193 EQVDKSSIDDGDQTWKDIITSKQGELIRNFMRNSASQLTIYG 318 +Q K++++D D KD IT +QGELI NF++N+ASQLT YG Sbjct: 537 KQAGKTAVEDSDMRSKDEITLQQGELIGNFLKNNASQLTFYG 578 Score = 48.1 bits (113), Expect(2) = 1e-12 Identities = 19/28 (67%), Positives = 26/28 (92%) Frame = +3 Query: 93 SEPIYEGEECILESRSTFYENQEPVYEG 176 SEPIYEGE+CIL+S +T Y+++EP+YEG Sbjct: 504 SEPIYEGEDCILDSITTTYQDREPIYEG 531 >ref|XP_012476036.1| PREDICTED: protein FAM63A-like isoform X2 [Gossypium raimondii] Length = 736 Score = 51.2 bits (121), Expect(2) = 1e-12 Identities = 23/42 (54%), Positives = 32/42 (76%) Frame = +1 Query: 193 EQVDKSSIDDGDQTWKDIITSKQGELIRNFMRNSASQLTIYG 318 +Q K++++D D KD IT +QGELI NF++N+ASQLT YG Sbjct: 523 KQAGKTAVEDSDMRSKDEITLQQGELIGNFLKNNASQLTFYG 564 Score = 48.1 bits (113), Expect(2) = 1e-12 Identities = 19/28 (67%), Positives = 26/28 (92%) Frame = +3 Query: 93 SEPIYEGEECILESRSTFYENQEPVYEG 176 SEPIYEGE+CIL+S +T Y+++EP+YEG Sbjct: 490 SEPIYEGEDCILDSITTTYQDREPIYEG 517 >ref|XP_006358850.1| PREDICTED: protein FAM63B-like [Solanum tuberosum] Length = 694 Score = 50.1 bits (118), Expect(2) = 1e-12 Identities = 25/42 (59%), Positives = 32/42 (76%) Frame = +1 Query: 193 EQVDKSSIDDGDQTWKDIITSKQGELIRNFMRNSASQLTIYG 318 EQV+ S D + KD IT K+GEL+RNF++NSASQLTI+G Sbjct: 477 EQVNGDSRDVPEIIVKDEITQKEGELVRNFLKNSASQLTIFG 518 Score = 49.3 bits (116), Expect(2) = 1e-12 Identities = 22/40 (55%), Positives = 32/40 (80%), Gaps = 4/40 (10%) Frame = +3 Query: 69 EDLETFSFS----EPIYEGEECILESRSTFYENQEPVYEG 176 ++ ETF+ S EP+YEGE+CILES +T Y+++EP+YEG Sbjct: 432 DEAETFNSSIDGDEPMYEGEDCILESATTSYQSREPMYEG 471 >ref|XP_009772020.1| PREDICTED: protein FAM63B [Nicotiana sylvestris] Length = 690 Score = 49.7 bits (117), Expect(2) = 2e-12 Identities = 23/40 (57%), Positives = 32/40 (80%), Gaps = 4/40 (10%) Frame = +3 Query: 69 EDLETFSFS----EPIYEGEECILESRSTFYENQEPVYEG 176 ++ ETF+ S EPIYEGE+CILES +T Y+++EP+YEG Sbjct: 430 DEPETFNSSIDDDEPIYEGEDCILESATTSYQSREPMYEG 469 Score = 48.5 bits (114), Expect(2) = 2e-12 Identities = 23/42 (54%), Positives = 31/42 (73%) Frame = +1 Query: 193 EQVDKSSIDDGDQTWKDIITSKQGELIRNFMRNSASQLTIYG 318 EQV+ S D + D IT ++GEL+RNF++NSASQLTI+G Sbjct: 475 EQVNGGSTDVPETIVNDEITQREGELVRNFLKNSASQLTIFG 516 >gb|KHN34990.1| Protein FAM63A [Glycine soja] Length = 721 Score = 50.1 bits (118), Expect(2) = 3e-12 Identities = 24/42 (57%), Positives = 32/42 (76%) Frame = +1 Query: 193 EQVDKSSIDDGDQTWKDIITSKQGELIRNFMRNSASQLTIYG 318 EQ DKS++ D KD +T +QGELI++F+RN+ASQLT YG Sbjct: 503 EQYDKSTLAAPDLRAKDELTPEQGELIKSFLRNNASQLTFYG 544 Score = 47.8 bits (112), Expect(2) = 3e-12 Identities = 19/28 (67%), Positives = 26/28 (92%) Frame = +3 Query: 93 SEPIYEGEECILESRSTFYENQEPVYEG 176 SEPIYEGEEC+L++R+ +E++EPVYEG Sbjct: 470 SEPIYEGEECVLDTRTGNFEDREPVYEG 497 >ref|XP_006440500.1| hypothetical protein CICLE_v10019075mg [Citrus clementina] gi|557542762|gb|ESR53740.1| hypothetical protein CICLE_v10019075mg [Citrus clementina] Length = 709 Score = 50.1 bits (118), Expect(2) = 3e-12 Identities = 21/28 (75%), Positives = 25/28 (89%) Frame = +3 Query: 93 SEPIYEGEECILESRSTFYENQEPVYEG 176 SEPIYEGEECIL+S + YE++EPVYEG Sbjct: 462 SEPIYEGEECILDSGTAMYEDREPVYEG 489 Score = 47.8 bits (112), Expect(2) = 3e-12 Identities = 22/42 (52%), Positives = 33/42 (78%) Frame = +1 Query: 193 EQVDKSSIDDGDQTWKDIITSKQGELIRNFMRNSASQLTIYG 318 EQ DKS++D + KD IT +QGELI++F++++A+QLT YG Sbjct: 495 EQADKSTLDACNVWSKDEITQQQGELIKSFLKSNANQLTFYG 536