BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Forsythia21_contig00019219
         (721 letters)

Database: ./nr 
           69,698,275 sequences; 24,982,196,650 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011078907.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   304   3e-80
ref|XP_011075249.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   296   6e-78
ref|XP_006351313.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   289   1e-75
ref|XP_004249262.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   289   1e-75
emb|CAN82223.1| hypothetical protein VITISV_011871 [Vitis vinifera]   287   4e-75
ref|XP_002278289.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   286   6e-75
ref|XP_009784415.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   285   2e-74
ref|XP_012828124.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   278   2e-72
emb|CDP14111.1| unnamed protein product [Coffea canephora]            276   9e-72
ref|XP_009597189.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   273   6e-71
ref|XP_009341215.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   261   4e-67
ref|XP_008394196.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   260   6e-67
ref|XP_008394194.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   260   6e-67
ref|XP_012086153.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   259   1e-66
ref|XP_007204337.1| hypothetical protein PRUPE_ppa005400mg [Prun...   258   2e-66
ref|XP_004306206.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   256   1e-65
ref|XP_008222118.1| PREDICTED: LOW QUALITY PROTEIN: cytoplasmic ...   254   3e-65
gb|KEH23632.1| cytoplasmic tRNA 2-thiolation protein [Medicago t...   253   8e-65
ref|XP_004493493.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   252   2e-64
ref|XP_011048047.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   250   5e-64

>ref|XP_011078907.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like [Sesamum
           indicum]
          Length = 473

 Score =  304 bits (779), Expect = 3e-80
 Identities = 160/250 (64%), Positives = 184/250 (73%), Gaps = 12/250 (4%)
 Frame = -3

Query: 716 MACNLATCQSGCYKDSDSGA------------IQERQPATEKGGGDLVFSANSRVCIKCK 573
           M+CN  TC+SGCY++  SG              QE    + +GG    +S +  +CIKCK
Sbjct: 1   MSCNSGTCESGCYREEYSGDDVRHNRPLVTGNSQESNGVSSRGG----YSNSHGLCIKCK 56

Query: 572 DNETIAATHPXXXXXXXXXXXXXXXSRFCADCFRSNLFGKFRFAVTSNAMISPSDKVLIA 393
            NETIA+TH                 RFCADCFRSNLFGKFRFAVTSNAMISPSD VL+A
Sbjct: 57  VNETIASTHQSVAAGGGDSI------RFCADCFRSNLFGKFRFAVTSNAMISPSDNVLVA 110

Query: 392 FSGGSSSRVAIQFVHEMQHKAQKNFDASRDKSLPVFGVGVAYIDESANFTVPSHDFDRAI 213
           FSGG  SRVA+QFVHEMQHK+QKNFDASRD+SLPVFGVGVAYIDES  F +PS+DFD  +
Sbjct: 111 FSGGPGSRVALQFVHEMQHKSQKNFDASRDRSLPVFGVGVAYIDESIIFPIPSNDFDSGL 170

Query: 212 DEMKLIVSNLAPPVKRFHVVPTESIYSSDTGAGRHKLMELINAVSDITGKEDLLEHLRMS 33
           DEMKLIVS+LAPP K+F VVPTESIYS D  AGR +L +L +AV+D+TGKEDLLEHLRM 
Sbjct: 171 DEMKLIVSSLAPPAKQFFVVPTESIYSPDCAAGRDQLTKLASAVNDVTGKEDLLEHLRML 230

Query: 32  SLQKIAVENG 3
           SLQKIA ENG
Sbjct: 231 SLQKIATENG 240


>ref|XP_011075249.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like [Sesamum
           indicum]
          Length = 483

 Score =  296 bits (759), Expect = 6e-78
 Identities = 162/245 (66%), Positives = 184/245 (75%), Gaps = 6/245 (2%)
 Frame = -3

Query: 719 KMACNLATCQSGCYKDSDS--GAIQERQPATEKG----GGDLVFSANSRVCIKCKDNETI 558
           KMACN ATCQSGCY D  S     Q+R P  E G    GG+  F+ +  +CIKCK NETI
Sbjct: 12  KMACNSATCQSGCYGDEHSVDEDDQKRPPVQEDGVSAGGGN--FNCDG-LCIKCKVNETI 68

Query: 557 AATHPXXXXXXXXXXXXXXXSRFCADCFRSNLFGKFRFAVTSNAMISPSDKVLIAFSGGS 378
           AATHP                RFCADCFRSNLFGKFRFAVTSNAMIS SD VL+AFSGG 
Sbjct: 69  AATHPAVSEGGGDRG------RFCADCFRSNLFGKFRFAVTSNAMISSSDNVLVAFSGGP 122

Query: 377 SSRVAIQFVHEMQHKAQKNFDASRDKSLPVFGVGVAYIDESANFTVPSHDFDRAIDEMKL 198
           SSRVA+QFVHEMQ KAQKNF+ASRD+ LPVFGVGVAYIDES  + V SHD+D AI++MKL
Sbjct: 123 SSRVALQFVHEMQQKAQKNFEASRDRLLPVFGVGVAYIDESTIYPVSSHDYDGAINQMKL 182

Query: 197 IVSNLAPPVKRFHVVPTESIYSSDTGAGRHKLMELINAVSDITGKEDLLEHLRMSSLQKI 18
           IV++LAPP K+F VVPT+SIYS D+  G+ +L EL  AVSDITGKEDL  HLRM SLQK+
Sbjct: 183 IVASLAPPAKQFFVVPTQSIYSHDSTDGKDRLTELFGAVSDITGKEDLQVHLRMLSLQKV 242

Query: 17  AVENG 3
           A+ENG
Sbjct: 243 ALENG 247


>ref|XP_006351313.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like [Solanum
           tuberosum]
          Length = 481

 Score =  289 bits (739), Expect = 1e-75
 Identities = 152/246 (61%), Positives = 178/246 (72%), Gaps = 8/246 (3%)
 Frame = -3

Query: 716 MACNLATCQSGCYKDSDSGAIQERQPATEKG--GGDLVFSANSR------VCIKCKDNET 561
           MACN  TCQSGCY+D  S   +E  P T+K     ++V S  S       +C+KCK N T
Sbjct: 1   MACNSGTCQSGCYRDDSSVGAEESPPLTKKTEVSNEVVNSRRSTEHLDDGICLKCKVNRT 60

Query: 560 IAATHPXXXXXXXXXXXXXXXSRFCADCFRSNLFGKFRFAVTSNAMISPSDKVLIAFSGG 381
           IAA                   RFCADCFRSNLFGKFR AVT+NAMISPSD VL+AFSGG
Sbjct: 61  IAAA---AAAEGGGDFGGGDGGRFCADCFRSNLFGKFRLAVTANAMISPSDNVLVAFSGG 117

Query: 380 SSSRVAIQFVHEMQHKAQKNFDASRDKSLPVFGVGVAYIDESANFTVPSHDFDRAIDEMK 201
           + SRVA+QF HEMQ KAQKN+DASRD++LPVFGVGVA+IDE A   V S + DRA++EMK
Sbjct: 118 TCSRVALQFTHEMQSKAQKNYDASRDRALPVFGVGVAFIDEKAISAVSSDNLDRAVEEMK 177

Query: 200 LIVSNLAPPVKRFHVVPTESIYSSDTGAGRHKLMELINAVSDITGKEDLLEHLRMSSLQK 21
           LIVS+LAPPVK+FHVVPTE+IYS   G  + +L ELIN V+D+TGKEDLLEH RM SLQK
Sbjct: 178 LIVSDLAPPVKQFHVVPTEAIYSLKAGDAKDRLNELINTVNDVTGKEDLLEHFRMLSLQK 237

Query: 20  IAVENG 3
           IA+ENG
Sbjct: 238 IAIENG 243


>ref|XP_004249262.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Solanum
           lycopersicum]
          Length = 478

 Score =  289 bits (739), Expect = 1e-75
 Identities = 152/246 (61%), Positives = 178/246 (72%), Gaps = 8/246 (3%)
 Frame = -3

Query: 716 MACNLATCQSGCYKDSDSGAIQERQPATEKG--GGDLVFSANSR------VCIKCKDNET 561
           MACN  TCQSGCY+D  S   +E  P T+K      +V S  S       +C+KCK N T
Sbjct: 1   MACNSGTCQSGCYRDDSSVGAEESPPLTKKTEVSNGVVNSRRSTEHLDDGICLKCKVNRT 60

Query: 560 IAATHPXXXXXXXXXXXXXXXSRFCADCFRSNLFGKFRFAVTSNAMISPSDKVLIAFSGG 381
           IAA                   RFCADCFRSNLFGKFR AVT+NAMISPSD VL+AFSGG
Sbjct: 61  IAAAD------GGGDFGGGDAGRFCADCFRSNLFGKFRLAVTANAMISPSDNVLVAFSGG 114

Query: 380 SSSRVAIQFVHEMQHKAQKNFDASRDKSLPVFGVGVAYIDESANFTVPSHDFDRAIDEMK 201
           + SRVA+QF HEMQ KAQKN+DASRD++LPVFGVGVA+IDE A   V S + DRA++EM+
Sbjct: 115 TCSRVALQFTHEMQSKAQKNYDASRDRALPVFGVGVAFIDEKAISAVSSDNLDRAVEEME 174

Query: 200 LIVSNLAPPVKRFHVVPTESIYSSDTGAGRHKLMELINAVSDITGKEDLLEHLRMSSLQK 21
           LIVS+LAPPVK+FHVVPTE+IYS D G  + +L ELIN V+D+TGKEDLLEH RM SLQK
Sbjct: 175 LIVSDLAPPVKQFHVVPTEAIYSLDAGDSKDRLNELINTVNDVTGKEDLLEHFRMLSLQK 234

Query: 20  IAVENG 3
           IA+ENG
Sbjct: 235 IAIENG 240


>emb|CAN82223.1| hypothetical protein VITISV_011871 [Vitis vinifera]
          Length = 747

 Score =  287 bits (735), Expect = 4e-75
 Identities = 146/241 (60%), Positives = 176/241 (73%), Gaps = 3/241 (1%)
 Frame = -3

Query: 716 MACNLATCQSGCYKDSDSGAIQERQPATEK---GGGDLVFSANSRVCIKCKDNETIAATH 546
           MAC    CQSGC++D D   + E++  T +    G D+    +  +C+KCK NET+A++ 
Sbjct: 1   MACGAGNCQSGCFRDDDDSVVTEKKKLTNRTLSSGSDIDLPNHHNLCLKCKVNETLASS- 59

Query: 545 PXXXXXXXXXXXXXXXSRFCADCFRSNLFGKFRFAVTSNAMISPSDKVLIAFSGGSSSRV 366
                            RFC DCFR NLFGKF+ AVTSNAMISP+D VL+AFSGG SSRV
Sbjct: 60  -------GTVSVDGDIGRFCFDCFRGNLFGKFKLAVTSNAMISPTDNVLVAFSGGPSSRV 112

Query: 365 AIQFVHEMQHKAQKNFDASRDKSLPVFGVGVAYIDESANFTVPSHDFDRAIDEMKLIVSN 186
           A+QFVHEMQHKAQKNFDASRD+SLPVFGVGVA++DESA   +PS +FD+AI +MK IVSN
Sbjct: 113 ALQFVHEMQHKAQKNFDASRDRSLPVFGVGVAFVDESAVSRIPSDEFDKAIQDMKSIVSN 172

Query: 185 LAPPVKRFHVVPTESIYSSDTGAGRHKLMELINAVSDITGKEDLLEHLRMSSLQKIAVEN 6
           LAPP K FHVVP ES+YSS+   GR +L EL+NAV D+TGKEDLL+HLRM SLQK A EN
Sbjct: 173 LAPPXKAFHVVPIESVYSSN---GRDRLEELLNAVKDVTGKEDLLQHLRMLSLQKNAYEN 229

Query: 5   G 3
           G
Sbjct: 230 G 230


>ref|XP_002278289.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Vitis vinifera]
           gi|297739656|emb|CBI29838.3| unnamed protein product
           [Vitis vinifera]
          Length = 468

 Score =  286 bits (733), Expect = 6e-75
 Identities = 146/241 (60%), Positives = 176/241 (73%), Gaps = 3/241 (1%)
 Frame = -3

Query: 716 MACNLATCQSGCYKDSDSGAIQERQPATEK---GGGDLVFSANSRVCIKCKDNETIAATH 546
           MAC    CQSGC++D D   + E++  T +    G D+    +  +C+KCK NET+A++ 
Sbjct: 1   MACGAGNCQSGCFRDDDDSVVTEKKKLTNRTLSSGSDIDLPNHHNLCLKCKVNETLASS- 59

Query: 545 PXXXXXXXXXXXXXXXSRFCADCFRSNLFGKFRFAVTSNAMISPSDKVLIAFSGGSSSRV 366
                            RFC DCFR NLFGKF+ AVTSNAMISP+D VL+AFSGG SSRV
Sbjct: 60  -------GTVSVDGDIGRFCFDCFRGNLFGKFKLAVTSNAMISPTDNVLVAFSGGPSSRV 112

Query: 365 AIQFVHEMQHKAQKNFDASRDKSLPVFGVGVAYIDESANFTVPSHDFDRAIDEMKLIVSN 186
           A+QFVHEMQHKAQKNFDASRD+SLPVFGVGVA++DESA   +PS +FD+AI +MK IVSN
Sbjct: 113 ALQFVHEMQHKAQKNFDASRDRSLPVFGVGVAFVDESAVSRIPSDEFDKAIQDMKSIVSN 172

Query: 185 LAPPVKRFHVVPTESIYSSDTGAGRHKLMELINAVSDITGKEDLLEHLRMSSLQKIAVEN 6
           LAPP K FHVVP ES+YSS+   GR +L EL+NAV D+TGKEDLL+HLRM SLQK A EN
Sbjct: 173 LAPPDKAFHVVPIESVYSSN---GRDRLEELLNAVKDVTGKEDLLQHLRMLSLQKNAYEN 229

Query: 5   G 3
           G
Sbjct: 230 G 230


>ref|XP_009784415.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Nicotiana
           sylvestris]
          Length = 478

 Score =  285 bits (729), Expect = 2e-74
 Identities = 151/245 (61%), Positives = 177/245 (72%), Gaps = 7/245 (2%)
 Frame = -3

Query: 716 MACNLATCQSGCYKDSDSGA-------IQERQPATEKGGGDLVFSANSRVCIKCKDNETI 558
           MACN  TCQSGCY+DS + A       I    P     G       ++ VC+KCK N+TI
Sbjct: 1   MACNSGTCQSGCYRDSSNEAEEHPPVKIANEAPNDVANGRKSTEQLSNFVCVKCKVNKTI 60

Query: 557 AATHPXXXXXXXXXXXXXXXSRFCADCFRSNLFGKFRFAVTSNAMISPSDKVLIAFSGGS 378
           AA                   RFCADCFRSNL+GKFRFAVTSNAMISPSD VL+AFSGG+
Sbjct: 61  AAA---ASADGGGDFIGGDRGRFCADCFRSNLYGKFRFAVTSNAMISPSDNVLVAFSGGT 117

Query: 377 SSRVAIQFVHEMQHKAQKNFDASRDKSLPVFGVGVAYIDESANFTVPSHDFDRAIDEMKL 198
            SRVA+QF HEMQ K+QKNFDAS D++LPVFGVGVA+IDE A   V S DFDRAI++MKL
Sbjct: 118 CSRVALQFTHEMQSKSQKNFDASPDRALPVFGVGVAFIDEKAISAVSSDDFDRAIEKMKL 177

Query: 197 IVSNLAPPVKRFHVVPTESIYSSDTGAGRHKLMELINAVSDITGKEDLLEHLRMSSLQKI 18
           IVS+LAPPVK+FHVVP E+IYS   G  + +L ELIN+VSD+TGKEDLLEHLRM SLQKI
Sbjct: 178 IVSDLAPPVKQFHVVPAEAIYSLKAGDAKDRLNELINSVSDVTGKEDLLEHLRMLSLQKI 237

Query: 17  AVENG 3
           A+++G
Sbjct: 238 ALKHG 242


>ref|XP_012828124.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Erythranthe
           guttatus] gi|604298732|gb|EYU18734.1| hypothetical
           protein MIMGU_mgv1a005684mg [Erythranthe guttata]
          Length = 474

 Score =  278 bits (712), Expect = 2e-72
 Identities = 153/248 (61%), Positives = 179/248 (72%), Gaps = 10/248 (4%)
 Frame = -3

Query: 716 MACNLATCQSGCYKDSDSGAIQERQ--PATEKGGGDLVFSAN------SRVCIKCKDNET 561
           MACN  TCQSG  +D  S     R+  P  E G G    S          VCIKCK N T
Sbjct: 1   MACNSGTCQSGSCRDEFSAGEDGRKQRPVPEIGNGSDHASPRRGESIAEGVCIKCKVNGT 60

Query: 560 IAATHPXXXXXXXXXXXXXXXSRFCADCFRSNLFGKFRFAVTSNAMISPSDKVLIAFSGG 381
           IA THP                RFCADCFRSNLFGKFRFAVTSNAMISP+D VL+AFSGG
Sbjct: 61  IATTHPAITGSGGDAG------RFCADCFRSNLFGKFRFAVTSNAMISPTDNVLVAFSGG 114

Query: 380 SSSRVAIQFVHEMQHKAQKNFDASR--DKSLPVFGVGVAYIDESANFTVPSHDFDRAIDE 207
           SSSRVA+QFVHEMQ KAQKNFDA++  ++ LPVF VGVAYIDE+  + VPS+DFDRA++E
Sbjct: 115 SSSRVALQFVHEMQQKAQKNFDATKSTERVLPVFRVGVAYIDETTVYPVPSNDFDRAVNE 174

Query: 206 MKLIVSNLAPPVKRFHVVPTESIYSSDTGAGRHKLMELINAVSDITGKEDLLEHLRMSSL 27
           M+LIVS+LAPP K+F VVPTESIYS+D  A R +L +L++AVSD+TGK+DL++HLRMS L
Sbjct: 175 MELIVSSLAPPEKQFFVVPTESIYSTDFTAERDRLTKLVDAVSDVTGKQDLMKHLRMSCL 234

Query: 26  QKIAVENG 3
           QK A ENG
Sbjct: 235 QKAAYENG 242


>emb|CDP14111.1| unnamed protein product [Coffea canephora]
          Length = 490

 Score =  276 bits (706), Expect = 9e-72
 Identities = 148/253 (58%), Positives = 182/253 (71%), Gaps = 15/253 (5%)
 Frame = -3

Query: 716 MACNLATCQSG-CYKDSDSGAIQERQP------ATEKGGGDLVFSANS--RVCIKCKDNE 564
           MACN ATCQSG CY++ +   + ++ P      ATE     L    NS    CIKCK  E
Sbjct: 1   MACNSATCQSGGCYREDNGDKVDDQFPQPPPAVATESTQQRLSNGKNSDSAFCIKCKLKE 60

Query: 563 TIAATHPXXXXXXXXXXXXXXXS------RFCADCFRSNLFGKFRFAVTSNAMISPSDKV 402
           ++AA                         RFC DCFRSNL+GKFR AVTSNA+ISPSD V
Sbjct: 61  SMAAVFSSSSASAQVTGGGGGRGGASDSSRFCGDCFRSNLYGKFRLAVTSNALISPSDNV 120

Query: 401 LIAFSGGSSSRVAIQFVHEMQHKAQKNFDASRDKSLPVFGVGVAYIDESANFTVPSHDFD 222
           L+AFSGG SSRVA+QFVHEMQ KAQKNFDASRD+SLPVFGVGVA+I ES++ ++P+  F 
Sbjct: 121 LVAFSGGPSSRVALQFVHEMQMKAQKNFDASRDRSLPVFGVGVAFIVESSSISIPADKFS 180

Query: 221 RAIDEMKLIVSNLAPPVKRFHVVPTESIYSSDTGAGRHKLMELINAVSDITGKEDLLEHL 42
           +A++EM+L+VSNLAPPVK FHVV TESIYSSD+  GR++L EL+NAV+D+TGKEDLL HL
Sbjct: 181 QAVEEMRLVVSNLAPPVKDFHVVSTESIYSSDSNNGRNRLKELLNAVADVTGKEDLLVHL 240

Query: 41  RMSSLQKIAVENG 3
           RM +LQK+A++NG
Sbjct: 241 RMLALQKVALKNG 253


>ref|XP_009597189.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2
           {ECO:0000255|HAMAP-Rule:MF_03054} [Nicotiana
           tomentosiformis]
          Length = 478

 Score =  273 bits (699), Expect = 6e-71
 Identities = 147/245 (60%), Positives = 170/245 (69%), Gaps = 7/245 (2%)
 Frame = -3

Query: 716 MACNLATCQSGCYKDSDSGA-------IQERQPATEKGGGDLVFSANSRVCIKCKDNETI 558
           MACN  TCQSGCY+DS + A       I    P     G       +  VC+KCK N+TI
Sbjct: 1   MACNSGTCQSGCYRDSSNEAEEHPPVKIANEAPNDVANGRKSTEQLSIAVCVKCKVNKTI 60

Query: 557 AATHPXXXXXXXXXXXXXXXSRFCADCFRSNLFGKFRFAVTSNAMISPSDKVLIAFSGGS 378
           A                    RFCADCFRSNL+GKFRFAVTSNAMISPSD VL+AFSGG+
Sbjct: 61  AVA---ASAEGGGDFSGGDSGRFCADCFRSNLYGKFRFAVTSNAMISPSDNVLVAFSGGT 117

Query: 377 SSRVAIQFVHEMQHKAQKNFDASRDKSLPVFGVGVAYIDESANFTVPSHDFDRAIDEMKL 198
            SRVA+QF HEMQ KAQKNFDAS D++LPVFGVGV +IDE A   V S + D AI++MK 
Sbjct: 118 CSRVALQFTHEMQSKAQKNFDASPDRALPVFGVGVVFIDEKAISAVSSDELDGAIEKMKH 177

Query: 197 IVSNLAPPVKRFHVVPTESIYSSDTGAGRHKLMELINAVSDITGKEDLLEHLRMSSLQKI 18
           IVS+LAPPVK+FHVVPTE+IYS   G  + +L EL+NAVSD+TGKEDLLEHL M SLQKI
Sbjct: 178 IVSDLAPPVKQFHVVPTEAIYSLKAGDAKDRLNELLNAVSDVTGKEDLLEHLLMLSLQKI 237

Query: 17  AVENG 3
           A+E G
Sbjct: 238 ALEYG 242


>ref|XP_009341215.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Pyrus x
           bretschneideri] gi|694427149|ref|XP_009341216.1|
           PREDICTED: cytoplasmic tRNA 2-thiolation protein 2
           [Pyrus x bretschneideri]
          Length = 463

 Score =  261 bits (666), Expect = 4e-67
 Identities = 132/240 (55%), Positives = 172/240 (71%), Gaps = 2/240 (0%)
 Frame = -3

Query: 716 MACNLATCQSGCYK--DSDSGAIQERQPATEKGGGDLVFSANSRVCIKCKDNETIAATHP 543
           MAC+ + C+S CYK    +   +++              ++NS +C+KCK N+ I++   
Sbjct: 1   MACSASGCESNCYKTQQEEDEDVKDNNNYKSISTNTRNNNSNSNLCLKCKANQPISSN-- 58

Query: 542 XXXXXXXXXXXXXXXSRFCADCFRSNLFGKFRFAVTSNAMISPSDKVLIAFSGGSSSRVA 363
                          +RFCADCFR NLFGKFRFAVTSNAMISP+D VL+AFSGG SSRVA
Sbjct: 59  --------TDGGGDDARFCADCFRGNLFGKFRFAVTSNAMISPTDNVLVAFSGGPSSRVA 110

Query: 362 IQFVHEMQHKAQKNFDASRDKSLPVFGVGVAYIDESANFTVPSHDFDRAIDEMKLIVSNL 183
           +QFVHEM +KAQKNFDASRD+SLPVFGVGVA++DES+ ++VPS + + AI++++LIV +L
Sbjct: 111 LQFVHEMHNKAQKNFDASRDRSLPVFGVGVAFVDESSVYSVPSGEVENAIEDIRLIVEDL 170

Query: 182 APPVKRFHVVPTESIYSSDTGAGRHKLMELINAVSDITGKEDLLEHLRMSSLQKIAVENG 3
           APP K  HVVP ES+YSSD+G GR +L +L+ AV D TGKEDLL H+RM +LQK+A E G
Sbjct: 171 APPTKELHVVPIESVYSSDSGDGRGRLNQLLEAVPDATGKEDLLLHMRMLALQKVASEKG 230


>ref|XP_008394196.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 isoform X2
           [Malus domestica]
          Length = 462

 Score =  260 bits (664), Expect = 6e-67
 Identities = 131/240 (54%), Positives = 173/240 (72%), Gaps = 2/240 (0%)
 Frame = -3

Query: 716 MACNLATCQSGCYK--DSDSGAIQERQPATEKGGGDLVFSANSRVCIKCKDNETIAATHP 543
           MAC+ + C+S CYK    +   +++              ++NS +C+KCK N+ I++   
Sbjct: 1   MACSASGCESNCYKTQQEEDEDVKDNNNYKSISTNTRNNNSNSXLCLKCKANQPISSN-- 58

Query: 542 XXXXXXXXXXXXXXXSRFCADCFRSNLFGKFRFAVTSNAMISPSDKVLIAFSGGSSSRVA 363
                          +RFCADCFR NLFGKFRFAVTSNAMISP+D VL+AFSGG SSRVA
Sbjct: 59  ---------TDGGDEARFCADCFRGNLFGKFRFAVTSNAMISPTDNVLVAFSGGPSSRVA 109

Query: 362 IQFVHEMQHKAQKNFDASRDKSLPVFGVGVAYIDESANFTVPSHDFDRAIDEMKLIVSNL 183
           +QFVHEM +KAQKNFDASRD+SLPVFGVGVA++DE++ ++VPS + + AI++++LIV +L
Sbjct: 110 LQFVHEMHNKAQKNFDASRDRSLPVFGVGVAFVDETSVYSVPSGEVENAIEDIRLIVEDL 169

Query: 182 APPVKRFHVVPTESIYSSDTGAGRHKLMELINAVSDITGKEDLLEHLRMSSLQKIAVENG 3
           APP K  HVVP ES+YSSD+G GR +L +L++AV D TGKEDLL H+RM +LQK+A E G
Sbjct: 170 APPTKELHVVPIESVYSSDSGDGRDRLNQLLDAVPDATGKEDLLLHMRMLALQKVASEKG 229


>ref|XP_008394194.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 isoform X1
           [Malus domestica] gi|658003365|ref|XP_008394195.1|
           PREDICTED: cytoplasmic tRNA 2-thiolation protein 2
           isoform X1 [Malus domestica]
          Length = 462

 Score =  260 bits (664), Expect = 6e-67
 Identities = 131/240 (54%), Positives = 173/240 (72%), Gaps = 2/240 (0%)
 Frame = -3

Query: 716 MACNLATCQSGCYK--DSDSGAIQERQPATEKGGGDLVFSANSRVCIKCKDNETIAATHP 543
           MAC+ + C+S CYK    +   +++              ++NS +C+KCK N+ I++   
Sbjct: 1   MACSASGCESNCYKTQQEEDEDVKDNNNYKSISTNTRNNNSNSXLCLKCKANQPISSN-- 58

Query: 542 XXXXXXXXXXXXXXXSRFCADCFRSNLFGKFRFAVTSNAMISPSDKVLIAFSGGSSSRVA 363
                          +RFCADCFR NLFGKFRFAVTSNAMISP+D VL+AFSGG SSRVA
Sbjct: 59  ---------TDGGDEARFCADCFRGNLFGKFRFAVTSNAMISPTDNVLVAFSGGPSSRVA 109

Query: 362 IQFVHEMQHKAQKNFDASRDKSLPVFGVGVAYIDESANFTVPSHDFDRAIDEMKLIVSNL 183
           +QFVHEM +KAQKNFDASRD+SLPVFGVGVA++DE++ ++VPS + + AI++++LIV +L
Sbjct: 110 LQFVHEMHNKAQKNFDASRDRSLPVFGVGVAFVDETSVYSVPSGEVENAIEDIRLIVEDL 169

Query: 182 APPVKRFHVVPTESIYSSDTGAGRHKLMELINAVSDITGKEDLLEHLRMSSLQKIAVENG 3
           APP K  HVVP ES+YSSD+G GR +L +L++AV D TGKEDLL H+RM +LQK+A E G
Sbjct: 170 APPTKELHVVPIESVYSSDSGDGRDRLNQLLDAVPDATGKEDLLLHMRMLALQKVASEKG 229


>ref|XP_012086153.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Jatropha
           curcas] gi|643713065|gb|KDP26051.1| hypothetical protein
           JCGZ_21084 [Jatropha curcas]
          Length = 465

 Score =  259 bits (661), Expect = 1e-66
 Identities = 131/238 (55%), Positives = 168/238 (70%)
 Frame = -3

Query: 716 MACNLATCQSGCYKDSDSGAIQERQPATEKGGGDLVFSANSRVCIKCKDNETIAATHPXX 537
           MACN ++C S CYK+ +          T++       +    +C+KCK N+ I+AT    
Sbjct: 1   MACNSSSCNSNCYKNDNVEQSLHSPRPTDRSSSYTSSNGQQNLCVKCKTNQPISATS--- 57

Query: 536 XXXXXXXXXXXXXSRFCADCFRSNLFGKFRFAVTSNAMISPSDKVLIAFSGGSSSRVAIQ 357
                        +RFC DCFRSNL+GKFR AVTS+AM++PSD VL+AFSGG +SRV +Q
Sbjct: 58  ----GGRGGGGDDTRFCGDCFRSNLYGKFRLAVTSHAMVTPSDNVLVAFSGGPASRVLLQ 113

Query: 356 FVHEMQHKAQKNFDASRDKSLPVFGVGVAYIDESANFTVPSHDFDRAIDEMKLIVSNLAP 177
           FV+EMQ +AQKNFDAS+D+SLPVFG+GVA+IDESA + VPS  FD  I EMKL+VSNLA 
Sbjct: 114 FVNEMQRRAQKNFDASKDRSLPVFGIGVAFIDESAVYPVPSEKFDEGIREMKLVVSNLAL 173

Query: 176 PVKRFHVVPTESIYSSDTGAGRHKLMELINAVSDITGKEDLLEHLRMSSLQKIAVENG 3
           P K+ HVVP E+IY+SD+  G+ KL +++NAV+D TGKEDLL HLR  +LQKIA ENG
Sbjct: 174 PTKKLHVVPIENIYTSDSIDGKDKLAKILNAVTDPTGKEDLLLHLRTLALQKIASENG 231


>ref|XP_007204337.1| hypothetical protein PRUPE_ppa005400mg [Prunus persica]
           gi|462399868|gb|EMJ05536.1| hypothetical protein
           PRUPE_ppa005400mg [Prunus persica]
          Length = 463

 Score =  258 bits (660), Expect = 2e-66
 Identities = 130/241 (53%), Positives = 169/241 (70%), Gaps = 3/241 (1%)
 Frame = -3

Query: 716 MACNLATCQSGCYKDSDSGAIQERQPATEKGGGDLVFS---ANSRVCIKCKDNETIAATH 546
           MAC+ + CQS CYK        +    T     ++  S   +NS +C+KCK N+ I++  
Sbjct: 1   MACSASACQSNCYKTQQEEEAVKANNNTNSFAANISSSNNNSNSGLCLKCKANQPISSNS 60

Query: 545 PXXXXXXXXXXXXXXXSRFCADCFRSNLFGKFRFAVTSNAMISPSDKVLIAFSGGSSSRV 366
                            RFC DCFR NLFGKFRFAVTSNAMI+P+D VL+AFSGG SSRV
Sbjct: 61  DGGDDA-----------RFCIDCFRGNLFGKFRFAVTSNAMIAPTDNVLVAFSGGPSSRV 109

Query: 365 AIQFVHEMQHKAQKNFDASRDKSLPVFGVGVAYIDESANFTVPSHDFDRAIDEMKLIVSN 186
           A+QFVHEM  KAQKNFDA RD+SLPVFGVGVA++DES+ ++ PS++ D+ I +++LIV N
Sbjct: 110 ALQFVHEMHSKAQKNFDACRDRSLPVFGVGVAFVDESSVYSAPSNETDKVIGDVRLIVEN 169

Query: 185 LAPPVKRFHVVPTESIYSSDTGAGRHKLMELINAVSDITGKEDLLEHLRMSSLQKIAVEN 6
           LAPP K  ++VP ES+YSSD+G GR +L +L++AV D TG+EDLL HLRM +LQK+A +N
Sbjct: 170 LAPPSKELYIVPIESVYSSDSGDGRERLKKLLDAVGDATGREDLLLHLRMLALQKVASQN 229

Query: 5   G 3
           G
Sbjct: 230 G 230


>ref|XP_004306206.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Fragaria vesca
           subsp. vesca]
          Length = 455

 Score =  256 bits (653), Expect = 1e-65
 Identities = 135/238 (56%), Positives = 170/238 (71%)
 Frame = -3

Query: 716 MACNLATCQSGCYKDSDSGAIQERQPATEKGGGDLVFSANSRVCIKCKDNETIAATHPXX 537
           MAC+ +TCQS CYKD       E  P T       +   ++ +C+KCK N  I+++    
Sbjct: 1   MACSASTCQSNCYKDQPD----EHDPDTNSVANRNI---SNGLCLKCKTNPPISSS---- 49

Query: 536 XXXXXXXXXXXXXSRFCADCFRSNLFGKFRFAVTSNAMISPSDKVLIAFSGGSSSRVAIQ 357
                        SRFC++CFR+NLFGKFRFAVTSNAMISPSD VL+AFSGG SSRVA++
Sbjct: 50  ----------ADDSRFCSECFRANLFGKFRFAVTSNAMISPSDNVLLAFSGGPSSRVALE 99

Query: 356 FVHEMQHKAQKNFDASRDKSLPVFGVGVAYIDESANFTVPSHDFDRAIDEMKLIVSNLAP 177
           FVHEM  KAQKNFDASRD+SLPVFGVGVA+IDE++ ++ PS++  +AI E+  IV  LAP
Sbjct: 100 FVHEMHSKAQKNFDASRDRSLPVFGVGVAFIDEASVYSSPSNELYKAIQEITAIVEELAP 159

Query: 176 PVKRFHVVPTESIYSSDTGAGRHKLMELINAVSDITGKEDLLEHLRMSSLQKIAVENG 3
           P K+ +VVP ES+YSS+ G GR +L  L++AV+D TGKEDLL HLRM +LQKIA ENG
Sbjct: 160 PAKQLYVVPIESVYSSECGDGRERLKRLLDAVTDPTGKEDLLLHLRMLALQKIASENG 217


>ref|XP_008222118.1| PREDICTED: LOW QUALITY PROTEIN: cytoplasmic tRNA 2-thiolation
           protein 2 [Prunus mume]
          Length = 464

 Score =  254 bits (649), Expect = 3e-65
 Identities = 131/247 (53%), Positives = 170/247 (68%), Gaps = 9/247 (3%)
 Frame = -3

Query: 716 MACNLATCQSGCYKDSDSGAIQERQPATEKGGGDLVFSAN---------SRVCIKCKDNE 564
           MAC+ + CQS CYK       QE + A +       F+AN         S +C+KCK N+
Sbjct: 1   MACSASACQSNCYKTQ-----QEEEEAVKDNSNTNSFAANNSSSNNNSNSGLCLKCKANQ 55

Query: 563 TIAATHPXXXXXXXXXXXXXXXSRFCADCFRSNLFGKFRFAVTSNAMISPSDKVLIAFSG 384
            I+++                  RFC DCFR NLFGKFR AVTSNAMI+P+D VL+AFSG
Sbjct: 56  PISSSSDGGDDA-----------RFCIDCFRGNLFGKFRLAVTSNAMIAPTDNVLVAFSG 104

Query: 383 GSSSRVAIQFVHEMQHKAQKNFDASRDKSLPVFGVGVAYIDESANFTVPSHDFDRAIDEM 204
           G SSRVA+QFVHEM  KAQKNFDA RD+SLPVFGVGVA++DES+ ++VP ++ D+ I ++
Sbjct: 105 GPSSRVALQFVHEMHSKAQKNFDACRDRSLPVFGVGVAFVDESSVYSVPYNETDKVIGDV 164

Query: 203 KLIVSNLAPPVKRFHVVPTESIYSSDTGAGRHKLMELINAVSDITGKEDLLEHLRMSSLQ 24
           +LIV NLAPP K  ++VP ES+YSSD+G GR +L +L++AV D TG+EDLL HLRM +LQ
Sbjct: 165 RLIVENLAPPSKELYIVPIESVYSSDSGDGRERLKKLLDAVGDATGREDLLLHLRMLALQ 224

Query: 23  KIAVENG 3
            +A +NG
Sbjct: 225 XVASQNG 231


>gb|KEH23632.1| cytoplasmic tRNA 2-thiolation protein [Medicago truncatula]
          Length = 458

 Score =  253 bits (646), Expect = 8e-65
 Identities = 133/239 (55%), Positives = 167/239 (69%), Gaps = 1/239 (0%)
 Frame = -3

Query: 716 MACN-LATCQSGCYKDSDSGAIQERQPATEKGGGDLVFSANSRVCIKCKDNETIAATHPX 540
           MACN +  CQSGCYKD +    Q     TE    +   S N  +CIKCK N+ I+     
Sbjct: 1   MACNGVGGCQSGCYKDEE----QTHDQPTESSETESKTSTN--ICIKCKLNDVISG---- 50

Query: 539 XXXXXXXXXXXXXXSRFCADCFRSNLFGKFRFAVTSNAMISPSDKVLIAFSGGSSSRVAI 360
                          RFCADCFRSNLFGKFR AVTSNAMISP+DKVL+AFSGG SSRVA+
Sbjct: 51  --------YGGIDDGRFCADCFRSNLFGKFRLAVTSNAMISPTDKVLVAFSGGPSSRVAL 102

Query: 359 QFVHEMQHKAQKNFDASRDKSLPVFGVGVAYIDESANFTVPSHDFDRAIDEMKLIVSNLA 180
           QFVH++Q +A KNF+ASRD+SLPVFGVGV +IDESA   +P+H+ + A++ + LIVS+LA
Sbjct: 103 QFVHDLQERAHKNFEASRDRSLPVFGVGVVFIDESAVLPIPAHEMEEAVEVISLIVSSLA 162

Query: 179 PPVKRFHVVPTESIYSSDTGAGRHKLMELINAVSDITGKEDLLEHLRMSSLQKIAVENG 3
           PP K  H VP E++YSSD+  G+ +L+EL+N VSD TG+ED+L  LRM +LQK+A E G
Sbjct: 163 PPRKELHFVPIETVYSSDSSDGKDRLIELMNVVSDTTGREDMLLSLRMLALQKVASEFG 221


>ref|XP_004493493.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Cicer
           arietinum]
          Length = 458

 Score =  252 bits (643), Expect = 2e-64
 Identities = 132/239 (55%), Positives = 170/239 (71%), Gaps = 1/239 (0%)
 Frame = -3

Query: 716 MACNLAT-CQSGCYKDSDSGAIQERQPATEKGGGDLVFSANSRVCIKCKDNETIAATHPX 540
           MACN A  CQSGCYKD +  + Q    +           ++S +CIKCK N+ I+     
Sbjct: 1   MACNGAGGCQSGCYKDEEPTSDQPTASSETDS------RSSSNLCIKCKLNDAISG---- 50

Query: 539 XXXXXXXXXXXXXXSRFCADCFRSNLFGKFRFAVTSNAMISPSDKVLIAFSGGSSSRVAI 360
                          RFCADCF+SNLFGKFRFAVTSNAMISP+DKVL+AFSGG SSRVA+
Sbjct: 51  --------YGGIDDGRFCADCFKSNLFGKFRFAVTSNAMISPTDKVLVAFSGGPSSRVAL 102

Query: 359 QFVHEMQHKAQKNFDASRDKSLPVFGVGVAYIDESANFTVPSHDFDRAIDEMKLIVSNLA 180
           QFVH+MQ +A KNF+ASRD+SLPVFGVGV +IDE+A   +PSH+ + A++ + LIVS+L+
Sbjct: 103 QFVHDMQERAHKNFEASRDRSLPVFGVGVVFIDETAVLPIPSHEMEEAVEVINLIVSSLS 162

Query: 179 PPVKRFHVVPTESIYSSDTGAGRHKLMELINAVSDITGKEDLLEHLRMSSLQKIAVENG 3
           PP K  H+VP E++YSSD+  G+ +L+EL+NAVSD TG+ED+L  LRM +LQK+A E G
Sbjct: 163 PPRKELHLVPIETVYSSDSTDGKERLIELMNAVSDPTGREDMLLCLRMLALQKVASEFG 221


>ref|XP_011048047.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Populus
           euphratica]
          Length = 458

 Score =  250 bits (639), Expect = 5e-64
 Identities = 130/238 (54%), Positives = 166/238 (69%)
 Frame = -3

Query: 716 MACNLATCQSGCYKDSDSGAIQERQPATEKGGGDLVFSANSRVCIKCKDNETIAATHPXX 537
           M+CN + CQS CYK+ D  +     P       D     ++ +C+KCK N  I       
Sbjct: 1   MSCNSSGCQSNCYKNGDQVSDSPPPPPPLAAAND-----SNNLCLKCKTNSPITP----- 50

Query: 536 XXXXXXXXXXXXXSRFCADCFRSNLFGKFRFAVTSNAMISPSDKVLIAFSGGSSSRVAIQ 357
                        +RFCADCFRSN++GKFR AVTS+AMISPSD VL+AFSGG SSRVA+Q
Sbjct: 51  ------GSLSGDEARFCADCFRSNIYGKFRLAVTSHAMISPSDNVLVAFSGGPSSRVALQ 104

Query: 356 FVHEMQHKAQKNFDASRDKSLPVFGVGVAYIDESANFTVPSHDFDRAIDEMKLIVSNLAP 177
           FVH +Q +AQKNF+AS+DKSLPVFGVGVA+IDESA + V S   D AI +M+ +VSNLAP
Sbjct: 105 FVHGLQDRAQKNFEASKDKSLPVFGVGVAFIDESAVYPVSSDKVDGAIQDMRSVVSNLAP 164

Query: 176 PVKRFHVVPTESIYSSDTGAGRHKLMELINAVSDITGKEDLLEHLRMSSLQKIAVENG 3
           P K+ HVVP E+IY+SD+  G+ +L +L++AV+D TGKEDLL HLRM +LQK+A +NG
Sbjct: 165 PAKQLHVVPIENIYASDSIDGKDRLTKLLDAVNDATGKEDLLLHLRMLALQKVASQNG 222