BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Forsythia21_contig00019218
         (1081 letters)

Database: ./nr 
           69,698,275 sequences; 24,982,196,650 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_011075249.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   345   3e-92
ref|XP_011078907.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   343   2e-91
ref|XP_002278289.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   337   9e-90
emb|CDP14111.1| unnamed protein product [Coffea canephora]            336   2e-89
ref|XP_012828124.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   330   1e-87
ref|XP_006351313.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   330   1e-87
ref|XP_004249262.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   326   2e-86
ref|XP_009784415.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   312   3e-82
ref|XP_006421388.1| hypothetical protein CICLE_v10004945mg [Citr...   310   2e-81
ref|XP_006489985.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   309   2e-81
ref|XP_012491368.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   307   1e-80
ref|XP_012491365.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   304   8e-80
ref|XP_009597189.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   304   8e-80
gb|KHG24162.1| Cytoplasmic tRNA 2-thiolation 2 -like protein [Go...   300   9e-79
ref|XP_004306206.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   298   4e-78
ref|XP_011048047.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   297   1e-77
ref|XP_002308030.2| hypothetical protein POPTR_0006s04980g [Popu...   295   3e-77
ref|XP_008222118.1| PREDICTED: LOW QUALITY PROTEIN: cytoplasmic ...   295   4e-77
ref|XP_007204337.1| hypothetical protein PRUPE_ppa005400mg [Prun...   294   9e-77
ref|XP_010672851.1| PREDICTED: cytoplasmic tRNA 2-thiolation pro...   287   1e-74

>ref|XP_011075249.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like [Sesamum
           indicum]
          Length = 483

 Score =  345 bits (886), Expect = 3e-92
 Identities = 173/247 (70%), Positives = 210/247 (85%)
 Frame = -1

Query: 988 VSVVQIAVENGYTKLVLGTCTSRIACRVLEATVKGQGYSLAADIQYVDARWEIPVVLPLR 809
           +S+ ++A+ENGYTK+V+GTCTS+IAC VLEATVKGQG+SLAADIQYVDARW+IPVVLPLR
Sbjct: 237 LSLQKVALENGYTKVVMGTCTSQIACHVLEATVKGQGFSLAADIQYVDARWKIPVVLPLR 296

Query: 808 DCISEELSMLCSLDSLKTVETFQQPHCGINGLISSFVKLLREENPSRESTIVRTAGKLKP 629
           DC+SEEL+M C LDSLKTVE FQ+P  GINGLISSFVKLL+EENPSRESTIVRTAGKL P
Sbjct: 297 DCLSEELNMCCILDSLKTVEAFQRPRSGINGLISSFVKLLQEENPSRESTIVRTAGKLTP 356

Query: 628 FSFNRIIEVNDCNGHSASQRRQKKYNLKPTESLPPETFCSICHSPLKKSDTTTLNATGNG 449
           F FNRI EV D NG  A++RRQKKYNLKP+ESLPPE++C IC++PLKK + T+ N   +G
Sbjct: 357 FCFNRIPEVGDNNGCLATKRRQKKYNLKPSESLPPESYCLICNNPLKKPNGTSSNHFHDG 416

Query: 448 EASVGIFEDTCSSCQYQILPQEPSSLESFRSLLPQQMISLAKNGSCDRQRWLREEMQEFL 269
            AS  I+  TCSSCQYQILP++P+ LE+F  LLPQ M +LAK+ S D  + LR+++Q+ L
Sbjct: 417 RASSEIYASTCSSCQYQILPKDPTLLENFFLLLPQSMTTLAKD-SRDNHKLLRDQIQDCL 475

Query: 268 LSDSEDG 248
           LSDS+DG
Sbjct: 476 LSDSDDG 482


>ref|XP_011078907.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like [Sesamum
            indicum]
          Length = 473

 Score =  343 bits (879), Expect = 2e-91
 Identities = 177/259 (68%), Positives = 205/259 (79%), Gaps = 2/259 (0%)
 Frame = -1

Query: 1015 GRHSLLHYA--VSVVQIAVENGYTKLVLGTCTSRIACRVLEATVKGQGYSLAADIQYVDA 842
            G+  LL +   +S+ +IA ENGY K+VLGTCTSRIAC VLEAT+KGQG+SL+ADIQYVDA
Sbjct: 219  GKEDLLEHLRMLSLQKIATENGYAKIVLGTCTSRIACHVLEATIKGQGFSLSADIQYVDA 278

Query: 841  RWEIPVVLPLRDCISEELSMLCSLDSLKTVETFQQPHCGINGLISSFVKLLREENPSRES 662
            RWEIPV+LPLRDC+SEEL+ LCSLDSLKT+E FQQP  GINGLI+SFVKLL+ ENPSRES
Sbjct: 279  RWEIPVMLPLRDCLSEELNTLCSLDSLKTIEVFQQPRSGINGLIASFVKLLQHENPSRES 338

Query: 661  TIVRTAGKLKPFSFNRIIEVNDCNGHSASQRRQKKYNLKPTESLPPETFCSICHSPLKKS 482
            TIVRTAGKL PF FNRI EV   NGH ASQRRQKKYNLKP ESLPPE+FC IC+SPLKKS
Sbjct: 339  TIVRTAGKLTPFCFNRIPEVGHDNGHLASQRRQKKYNLKPNESLPPESFCIICNSPLKKS 398

Query: 481  DTTTLNATGNGEASVGIFEDTCSSCQYQILPQEPSSLESFRSLLPQQMISLAKNGSCDRQ 302
              T  N   +GE         CSSCQYQILP++ SSLE F SLLPQ M + A++ S   +
Sbjct: 399  GNTIFNDLDDGETRAAA---ACSSCQYQILPRDLSSLEYFYSLLPQSMCARARDSSISHE 455

Query: 301  RWLREEMQEFLLSDSEDGT 245
              LR+++Q+ LLSDS DGT
Sbjct: 456  -LLRDQIQDCLLSDSNDGT 473


>ref|XP_002278289.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Vitis vinifera]
            gi|297739656|emb|CBI29838.3| unnamed protein product
            [Vitis vinifera]
          Length = 468

 Score =  337 bits (864), Expect = 9e-90
 Identities = 169/260 (65%), Positives = 206/260 (79%), Gaps = 3/260 (1%)
 Frame = -1

Query: 1015 GRHSLLHYA--VSVVQIAVENGYTKLVLGTCTSRIACRVLEATVKGQGYSLAADIQYVDA 842
            G+  LL +   +S+ + A ENGYTKL+LG+CTSRIAC V+ ATVKGQGYSL ADIQYVDA
Sbjct: 209  GKEDLLQHLRMLSLQKNAYENGYTKLLLGSCTSRIACHVISATVKGQGYSLPADIQYVDA 268

Query: 841  RWEIPVVLPLRDCISEELSMLCSLDSLKTVETFQQPHCGINGLISSFVKLLREENPSRES 662
            RWEIPVVLPLRDC+++EL+MLC LD LKT+E    PH GINGL++SFV LL+EENPSRE 
Sbjct: 269  RWEIPVVLPLRDCLAQELNMLCRLDGLKTMEVLNSPHSGINGLVASFVTLLQEENPSREC 328

Query: 661  TIVRTAGKLKPFSFNRIIEVNDCNGHSASQRRQKKYNLKPTESLPPETFCSICHSPLKKS 482
            TIVRTAGKL PF FN+I E +D N H AS+RRQKK+NLKP ES+PPE++C IC+SPL KS
Sbjct: 329  TIVRTAGKLTPFHFNQIPESDDSNVHLASRRRQKKFNLKPNESVPPESYCPICNSPLSKS 388

Query: 481  DTTTLNATGNGEASVGIFEDT-CSSCQYQILPQEPSSLESFRSLLPQQMISLAKNGSCDR 305
            D   L++ G+ + +  IF  T C SCQ+QILP+EP S+E F SLLPQQ +  AK+ S   
Sbjct: 389  DHINLSSLGSDQTNADIFGATCCPSCQFQILPKEPVSMEHFYSLLPQQFVVQAKDRSFSM 448

Query: 304  QRWLREEMQEFLLSDSEDGT 245
            QR LRE++Q+FLLSDSEDGT
Sbjct: 449  QRQLREQIQDFLLSDSEDGT 468


>emb|CDP14111.1| unnamed protein product [Coffea canephora]
          Length = 490

 Score =  336 bits (862), Expect = 2e-89
 Identities = 167/254 (65%), Positives = 202/254 (79%), Gaps = 1/254 (0%)
 Frame = -1

Query: 1003 LLHYAVSVVQ-IAVENGYTKLVLGTCTSRIACRVLEATVKGQGYSLAADIQYVDARWEIP 827
            L+H  +  +Q +A++NGYTK++LGTCTSRIACRVLEATVKGQGYSLAADIQYVDARWEIP
Sbjct: 237  LVHLRMLALQKVALKNGYTKVLLGTCTSRIACRVLEATVKGQGYSLAADIQYVDARWEIP 296

Query: 826  VVLPLRDCISEELSMLCSLDSLKTVETFQQPHCGINGLISSFVKLLREENPSRESTIVRT 647
            V+LPLRDC+++EL+++CSLDSLKTVE     H  IN LI+SFVKLL+EENPSRESTIVRT
Sbjct: 297  VILPLRDCLAQELNIMCSLDSLKTVEVHNDHHSSINALITSFVKLLQEENPSRESTIVRT 356

Query: 646  AGKLKPFSFNRIIEVNDCNGHSASQRRQKKYNLKPTESLPPETFCSICHSPLKKSDTTTL 467
            AGKL PF FNR+ E +DCN   ASQRRQKKYNLKP ESLPPE+FC IC+SPLKKSD  + 
Sbjct: 357  AGKLTPFHFNRMPEPDDCNMQLASQRRQKKYNLKPNESLPPESFCRICNSPLKKSDVKSY 416

Query: 466  NATGNGEASVGIFEDTCSSCQYQILPQEPSSLESFRSLLPQQMISLAKNGSCDRQRWLRE 287
                   ++  I    C+SCQ+QILP++ S LE F SLLPQQM++  +       RWLRE
Sbjct: 417  KLENQIMSAEDISNGCCTSCQFQILPKDISLLEHFYSLLPQQMVARGEYSCLGEDRWLRE 476

Query: 286  EMQEFLLSDSEDGT 245
            ++Q+ LLSD+EDGT
Sbjct: 477  QIQDCLLSDTEDGT 490


>ref|XP_012828124.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Erythranthe
            guttatus] gi|604298732|gb|EYU18734.1| hypothetical
            protein MIMGU_mgv1a005684mg [Erythranthe guttata]
          Length = 474

 Score =  330 bits (846), Expect = 1e-87
 Identities = 175/259 (67%), Positives = 206/259 (79%), Gaps = 2/259 (0%)
 Frame = -1

Query: 1015 GRHSLL-HYAVSVVQ-IAVENGYTKLVLGTCTSRIACRVLEATVKGQGYSLAADIQYVDA 842
            G+  L+ H  +S +Q  A ENGYTK+V+GTCTS+IAC VLEATVKGQG+SLAADIQYVDA
Sbjct: 221  GKQDLMKHLRMSCLQKAAYENGYTKIVMGTCTSQIACHVLEATVKGQGFSLAADIQYVDA 280

Query: 841  RWEIPVVLPLRDCISEELSMLCSLDSLKTVETFQQPHCGINGLISSFVKLLREENPSRES 662
            RW+IPVVLPLRDC SEEL+MLCSLDSLKTVE+ +QP  GINGLISSFVKLL+ ENPSRES
Sbjct: 281  RWKIPVVLPLRDCPSEELNMLCSLDSLKTVESLEQPRSGINGLISSFVKLLQVENPSRES 340

Query: 661  TIVRTAGKLKPFSFNRIIEVNDCNGHSASQRRQKKYNLKPTESLPPETFCSICHSPLKKS 482
            TIVRTAGKL PFSFN   EV D   H AS+RRQKKYNLKP ESLP E+FC IC+SPLKK+
Sbjct: 341  TIVRTAGKLTPFSFNTAPEVGDVTTHLASKRRQKKYNLKPDESLPTESFCLICNSPLKKT 400

Query: 481  DTTTLNATGNGEASVGIFEDTCSSCQYQILPQEPSSLESFRSLLPQQMISLAKNGSCDRQ 302
                 N   +GE    I+  TCSSCQ+QILP++ SS+E F SLLPQ M +L K  + D Q
Sbjct: 401  -----NDFHHGETHAEIYSGTCSSCQFQILPKDNSSMEYFCSLLPQSMTTLPKYTN-DNQ 454

Query: 301  RWLREEMQEFLLSDSEDGT 245
            + LRE++Q+ LLS+S+DGT
Sbjct: 455  KLLREQIQDCLLSESDDGT 473


>ref|XP_006351313.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like [Solanum
            tuberosum]
          Length = 481

 Score =  330 bits (846), Expect = 1e-87
 Identities = 172/260 (66%), Positives = 205/260 (78%), Gaps = 3/260 (1%)
 Frame = -1

Query: 1015 GRHSLLHY--AVSVVQIAVENGYTKLVLGTCTSRIACRVLEATVKGQGYSLAADIQYVDA 842
            G+  LL +   +S+ +IA+ENGYTK++LGTCTSRIAC VLEATVKG+GYSLAADIQYVDA
Sbjct: 222  GKEDLLEHFRMLSLQKIAIENGYTKILLGTCTSRIACHVLEATVKGRGYSLAADIQYVDA 281

Query: 841  RWEIPVVLPLRDCISEELSMLCSLDSLKTVETFQQPHCGINGLISSFVKLLREENPSRES 662
            RW+IPVVLPLRDC   EL+MLCSLDSLKTVE F     GINGLISSFVKLL+EENPSRES
Sbjct: 282  RWKIPVVLPLRDCPIHELNMLCSLDSLKTVEVFNGTRAGINGLISSFVKLLQEENPSRES 341

Query: 661  TIVRTAGKLKPFSFNRIIEVNDCNGHSASQRRQKKYNLKPTESLPPETFCSICHSPLKKS 482
            TI+RTAGKL PF FNR+ E ND NG  AS+RRQKKYNLK  ++LPPE+FC IC+SPLK S
Sbjct: 342  TIMRTAGKLTPFHFNRMPEDNDYNGQVASRRRQKKYNLKTKDALPPESFCPICNSPLKIS 401

Query: 481  DTTTLNATGNGEASV-GIFEDTCSSCQYQILPQEPSSLESFRSLLPQQMISLAKNGSCDR 305
            +  T     NG  +  GI    C+SCQ+Q+LP +PSSLE F SLLP  M+S A++G    
Sbjct: 402  NFGTSVTFDNGRTNPNGIGAACCASCQFQVLPNDPSSLEHFISLLPPSMVSEAEDGDRLS 461

Query: 304  QRWLREEMQEFLLSDSEDGT 245
            QR LRE++++ LLSD+EDGT
Sbjct: 462  QRQLREQIEDCLLSDNEDGT 481


>ref|XP_004249262.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Solanum
            lycopersicum]
          Length = 478

 Score =  326 bits (835), Expect = 2e-86
 Identities = 171/260 (65%), Positives = 203/260 (78%), Gaps = 3/260 (1%)
 Frame = -1

Query: 1015 GRHSLLHY--AVSVVQIAVENGYTKLVLGTCTSRIACRVLEATVKGQGYSLAADIQYVDA 842
            G+  LL +   +S+ +IA+ENGYTK++LGTCTSRIAC VLEATVKG+GYSLAADIQYVDA
Sbjct: 219  GKEDLLEHFRMLSLQKIAIENGYTKILLGTCTSRIACHVLEATVKGRGYSLAADIQYVDA 278

Query: 841  RWEIPVVLPLRDCISEELSMLCSLDSLKTVETFQQPHCGINGLISSFVKLLREENPSRES 662
            RW+IPVVLPLRDC   EL+MLCSLDSLKTVE F     GINGLISSFVKLL+EENPSRES
Sbjct: 279  RWKIPVVLPLRDCPIHELNMLCSLDSLKTVEVFNGTRAGINGLISSFVKLLQEENPSRES 338

Query: 661  TIVRTAGKLKPFSFNRIIEVNDCNGHSASQRRQKKYNLKPTESLPPETFCSICHSPLKKS 482
            TI+RTAGKL PF FNR+ E ND NG  AS+RRQKKYNLK  ++LPPE+FC IC+SPLK S
Sbjct: 339  TIMRTAGKLTPFHFNRMPEDNDYNGQVASRRRQKKYNLKTKDALPPESFCPICNSPLKIS 398

Query: 481  DTTTLNATGNGEASV-GIFEDTCSSCQYQILPQEPSSLESFRSLLPQQMISLAKNGSCDR 305
               T     NG+ +  GI    C+SCQ+Q+LP +PSSLE F SLLP  M+S A +     
Sbjct: 399  SFGTSINFENGKTNPNGIGAACCASCQFQVLPNDPSSLEHFISLLPPSMVSQAGDNDRLS 458

Query: 304  QRWLREEMQEFLLSDSEDGT 245
            QR LRE++++ LLSD+EDGT
Sbjct: 459  QRQLREQIEDCLLSDNEDGT 478


>ref|XP_009784415.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Nicotiana
            sylvestris]
          Length = 478

 Score =  312 bits (799), Expect = 3e-82
 Identities = 162/258 (62%), Positives = 201/258 (77%), Gaps = 3/258 (1%)
 Frame = -1

Query: 1015 GRHSLLHYA--VSVVQIAVENGYTKLVLGTCTSRIACRVLEATVKGQGYSLAADIQYVDA 842
            G+  LL +   +S+ +IA+++GYTK++LGTCTSRIAC VLEATVKG+GYSLAADIQYVDA
Sbjct: 221  GKEDLLEHLRMLSLQKIALKHGYTKVLLGTCTSRIACHVLEATVKGRGYSLAADIQYVDA 280

Query: 841  RWEIPVVLPLRDCISEELSMLCSLDSLKTVETFQQPHCGINGLISSFVKLLREENPSRES 662
            RW+IPVVLPLRDC+  EL+ LCSLDSL TVE F +   GINGL+SSFVKLL+EENPSRES
Sbjct: 281  RWKIPVVLPLRDCLIHELNTLCSLDSLMTVEVFNRARAGINGLVSSFVKLLQEENPSRES 340

Query: 661  TIVRTAGKLKPFSFNRIIEVNDCNGHSASQRRQKKYNLKPTESLPPETFCSICHSPLKKS 482
            TI+RTAGKL PF FNR+ E N+ N   ASQRRQKKY+LK  ++LPPE FC IC SPLK S
Sbjct: 341  TIMRTAGKLTPFHFNRMSEDNNYNDQVASQRRQKKYSLKTNDALPPELFCPICSSPLKIS 400

Query: 481  DTTTLNATGNGEASV-GIFEDTCSSCQYQILPQEPSSLESFRSLLPQQMISLAKNGSCDR 305
               T  +  N + S+  I    C+SCQ+Q+LP++PSSLE F SLLP  M+S A++G    
Sbjct: 401  TFGTSISFDNAKTSLNAIGAACCASCQFQVLPKDPSSLEHFISLLPPSMVSQAEDGDHLS 460

Query: 304  QRWLREEMQEFLLSDSED 251
            QR LRE++++ LLSD+ED
Sbjct: 461  QRQLREQIEDCLLSDNED 478


>ref|XP_006421388.1| hypothetical protein CICLE_v10004945mg [Citrus clementina]
           gi|557523261|gb|ESR34628.1| hypothetical protein
           CICLE_v10004945mg [Citrus clementina]
          Length = 455

 Score =  310 bits (793), Expect = 2e-81
 Identities = 152/246 (61%), Positives = 194/246 (78%)
 Frame = -1

Query: 988 VSVVQIAVENGYTKLVLGTCTSRIACRVLEATVKGQGYSLAADIQYVDARWEIPVVLPLR 809
           +S+ + A ENGY +L+LG CTSRIAC V+ ATVKG+GYSL ADIQY DARWEIPVVLPLR
Sbjct: 208 LSLQKFASENGYNRLLLGLCTSRIACHVITATVKGRGYSLPADIQYADARWEIPVVLPLR 267

Query: 808 DCISEELSMLCSLDSLKTVETFQQPHCGINGLISSFVKLLREENPSRESTIVRTAGKLKP 629
           DC+++EL+MLC LD LKTVE   Q H GINGL+SSFVK+L+EENPSRESTI+RTAGKL P
Sbjct: 268 DCLAQELNMLCQLDCLKTVELLNQTHSGINGLVSSFVKILQEENPSRESTIMRTAGKLTP 327

Query: 628 FSFNRIIEVNDCNGHSASQRRQKKYNLKPTESLPPETFCSICHSPLKKSDTTTLNATGNG 449
           F FN+I E+ND +   ASQRRQK++NLKP ES+  E+FCS+C+SPL +SD T+L++  N 
Sbjct: 328 FHFNKIPELNDSSVPLASQRRQKRFNLKPNESISSESFCSLCYSPLNQSDLTSLSSHDNC 387

Query: 448 EASVGIFEDTCSSCQYQILPQEPSSLESFRSLLPQQMISLAKNGSCDRQRWLREEMQEFL 269
           + S       CSSC++QI P++PSS+E F SLLP+ +++ AK+        LRE++Q+FL
Sbjct: 388 KNSDIFVAACCSSCRFQIFPKDPSSMEKFYSLLPEPLVARAKHVRNGDSSLLREQIQDFL 447

Query: 268 LSDSED 251
           LSDSED
Sbjct: 448 LSDSED 453


>ref|XP_006489985.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like [Citrus
           sinensis]
          Length = 455

 Score =  309 bits (792), Expect = 2e-81
 Identities = 152/246 (61%), Positives = 193/246 (78%)
 Frame = -1

Query: 988 VSVVQIAVENGYTKLVLGTCTSRIACRVLEATVKGQGYSLAADIQYVDARWEIPVVLPLR 809
           +S+ + A ENG+ +L+LG CTSRIAC V+ ATVKGQGYSL ADIQY DARWEIPVVLPLR
Sbjct: 208 LSLQKFASENGFNRLLLGLCTSRIACHVITATVKGQGYSLPADIQYADARWEIPVVLPLR 267

Query: 808 DCISEELSMLCSLDSLKTVETFQQPHCGINGLISSFVKLLREENPSRESTIVRTAGKLKP 629
           DC+++EL+MLC LD LKTVE   Q H GINGL+SSFVK+L+EENPSRESTI+RTAGKL P
Sbjct: 268 DCLAQELNMLCQLDCLKTVELLNQTHSGINGLVSSFVKILQEENPSRESTIMRTAGKLTP 327

Query: 628 FSFNRIIEVNDCNGHSASQRRQKKYNLKPTESLPPETFCSICHSPLKKSDTTTLNATGNG 449
           F FN+I E+ND +   ASQRRQK++NLKP ES+  E+FCS+C+SPL +SD T+L++  N 
Sbjct: 328 FHFNKIPELNDSSVPLASQRRQKRFNLKPNESISSESFCSLCYSPLNQSDLTSLSSHDNC 387

Query: 448 EASVGIFEDTCSSCQYQILPQEPSSLESFRSLLPQQMISLAKNGSCDRQRWLREEMQEFL 269
           + S       CSSC++QI P +PSS+E F SLLP+ +++ AK+        LRE++Q+FL
Sbjct: 388 KNSDIFVAACCSSCRFQIFPNDPSSMEKFYSLLPEPLVARAKHVRNGDSSLLREQIQDFL 447

Query: 268 LSDSED 251
           LSDSED
Sbjct: 448 LSDSED 453


>ref|XP_012491368.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 isoform X2
            [Gossypium raimondii] gi|763776011|gb|KJB43134.1|
            hypothetical protein B456_007G186200 [Gossypium
            raimondii] gi|763776014|gb|KJB43137.1| hypothetical
            protein B456_007G186200 [Gossypium raimondii]
          Length = 460

 Score =  307 bits (786), Expect = 1e-80
 Identities = 156/257 (60%), Positives = 196/257 (76%), Gaps = 2/257 (0%)
 Frame = -1

Query: 1015 GRHSLL-HYAVSVVQ-IAVENGYTKLVLGTCTSRIACRVLEATVKGQGYSLAADIQYVDA 842
            G+  LL H  + ++Q IA ENGYT++VLG+CTSRIAC V+ ATVKGQGYSL+ADIQYVD+
Sbjct: 202  GKEDLLSHLRILLLQKIASENGYTRIVLGSCTSRIACHVISATVKGQGYSLSADIQYVDS 261

Query: 841  RWEIPVVLPLRDCISEELSMLCSLDSLKTVETFQQPHCGINGLISSFVKLLREENPSRES 662
            RWEIPVVLPLRDC ++EL+ LCSLD LK VE    P  GINGL+SSFVK+L+EENPSRE 
Sbjct: 262  RWEIPVVLPLRDCPAQELNTLCSLDGLKIVELLNGPCSGINGLVSSFVKVLQEENPSREC 321

Query: 661  TIVRTAGKLKPFSFNRIIEVNDCNGHSASQRRQKKYNLKPTESLPPETFCSICHSPLKKS 482
            TIVRTAGKL PF FNR+ E++D N  SA++R QK+Y LKP  SL  ++FC IC+SPLKKS
Sbjct: 322  TIVRTAGKLTPFHFNRVPEIHDSNVPSATRRHQKRYTLKPNGSLSSDSFCPICNSPLKKS 381

Query: 481  DTTTLNATGNGEASVGIFEDTCSSCQYQILPQEPSSLESFRSLLPQQMISLAKNGSCDRQ 302
            +  +   +   + +  +F   CSSCQ+QILP++PS +E F SLLPQQMI+ AK+G     
Sbjct: 382  NFPSSLRSHGSQQNPDLFASACSSCQFQILPKDPSLMEEFLSLLPQQMITKAKHGDQGNF 441

Query: 301  RWLREEMQEFLLSDSED 251
              LRE++QEFL SD E+
Sbjct: 442  SLLREQIQEFLFSDGEN 458


>ref|XP_012491365.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 isoform X1
            [Gossypium raimondii] gi|823191127|ref|XP_012491367.1|
            PREDICTED: cytoplasmic tRNA 2-thiolation protein 2
            isoform X1 [Gossypium raimondii]
            gi|763776012|gb|KJB43135.1| hypothetical protein
            B456_007G186200 [Gossypium raimondii]
            gi|763776013|gb|KJB43136.1| hypothetical protein
            B456_007G186200 [Gossypium raimondii]
          Length = 461

 Score =  304 bits (778), Expect = 8e-80
 Identities = 157/258 (60%), Positives = 197/258 (76%), Gaps = 3/258 (1%)
 Frame = -1

Query: 1015 GRHSLL-HYAVSVVQ-IAVENGYTKLVLGTCTSRIACRVLEATVKGQGYSLAADIQYVDA 842
            G+  LL H  + ++Q IA ENGYT++VLG+CTSRIAC V+ ATVKGQGYSL+ADIQYVD+
Sbjct: 202  GKEDLLSHLRILLLQKIASENGYTRIVLGSCTSRIACHVISATVKGQGYSLSADIQYVDS 261

Query: 841  RWEIPVVLPLRDCISEELSMLCSLD-SLKTVETFQQPHCGINGLISSFVKLLREENPSRE 665
            RWEIPVVLPLRDC ++EL+ LCSLD SLK VE    P  GINGL+SSFVK+L+EENPSRE
Sbjct: 262  RWEIPVVLPLRDCPAQELNTLCSLDGSLKIVELLNGPCSGINGLVSSFVKVLQEENPSRE 321

Query: 664  STIVRTAGKLKPFSFNRIIEVNDCNGHSASQRRQKKYNLKPTESLPPETFCSICHSPLKK 485
             TIVRTAGKL PF FNR+ E++D N  SA++R QK+Y LKP  SL  ++FC IC+SPLKK
Sbjct: 322  CTIVRTAGKLTPFHFNRVPEIHDSNVPSATRRHQKRYTLKPNGSLSSDSFCPICNSPLKK 381

Query: 484  SDTTTLNATGNGEASVGIFEDTCSSCQYQILPQEPSSLESFRSLLPQQMISLAKNGSCDR 305
            S+  +   +   + +  +F   CSSCQ+QILP++PS +E F SLLPQQMI+ AK+G    
Sbjct: 382  SNFPSSLRSHGSQQNPDLFASACSSCQFQILPKDPSLMEEFLSLLPQQMITKAKHGDQGN 441

Query: 304  QRWLREEMQEFLLSDSED 251
               LRE++QEFL SD E+
Sbjct: 442  FSLLREQIQEFLFSDGEN 459


>ref|XP_009597189.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2
            {ECO:0000255|HAMAP-Rule:MF_03054} [Nicotiana
            tomentosiformis]
          Length = 478

 Score =  304 bits (778), Expect = 8e-80
 Identities = 159/258 (61%), Positives = 196/258 (75%), Gaps = 3/258 (1%)
 Frame = -1

Query: 1015 GRHSLLHYAV--SVVQIAVENGYTKLVLGTCTSRIACRVLEATVKGQGYSLAADIQYVDA 842
            G+  LL + +  S+ +IA+E GYTK++LGTCTSRIAC VLEATVKG+GYSLAADIQYVD 
Sbjct: 221  GKEDLLEHLLMLSLQKIALEYGYTKVLLGTCTSRIACHVLEATVKGRGYSLAADIQYVDT 280

Query: 841  RWEIPVVLPLRDCISEELSMLCSLDSLKTVETFQQPHCGINGLISSFVKLLREENPSRES 662
            RW+IPVVLPLRDC+  EL+MLCSLDSL TVE F     GINGL+SSFVKLL+EENPSRES
Sbjct: 281  RWKIPVVLPLRDCLIHELNMLCSLDSLMTVEVFNGTRAGINGLVSSFVKLLQEENPSRES 340

Query: 661  TIVRTAGKLKPFSFNRIIEVNDCNGHSASQRRQKKYNLKPTESLPPETFCSICHSPLKKS 482
            TI+RTAGKL PF FNR+ E N+ N   ASQRRQKKY+LK  ++LPPE+FC IC SPLK  
Sbjct: 341  TIMRTAGKLTPFHFNRMSEDNNYNDQVASQRRQKKYSLKTNDALPPESFCPICSSPLKIF 400

Query: 481  DTTTLNATGNGEASV-GIFEDTCSSCQYQILPQEPSSLESFRSLLPQQMISLAKNGSCDR 305
               T  +  N + S+  I    C+SC +Q+LP++P SLE F SLLP  M+  A++G    
Sbjct: 401  TFGTSISFDNAKTSLDAIGAACCASCHFQVLPKDPLSLEHFISLLPPSMVFQAEDGDHLS 460

Query: 304  QRWLREEMQEFLLSDSED 251
            QR LRE++++ LLSD+ED
Sbjct: 461  QRQLREQIEDCLLSDNED 478


>gb|KHG24162.1| Cytoplasmic tRNA 2-thiolation 2 -like protein [Gossypium arboreum]
          Length = 461

 Score =  300 bits (769), Expect = 9e-79
 Identities = 155/244 (63%), Positives = 192/244 (78%), Gaps = 2/244 (0%)
 Frame = -1

Query: 976 QIAVENGYTKLVLGTCTSRIACRVLEATVKGQGYSLAADIQYVDARWEIPVVLPLRDCIS 797
           +IA ENGYT++VLGTCTSRIAC V+ ATVKGQGYSL+ADIQYVD+RWEIPVVLPLRDC +
Sbjct: 217 KIASENGYTRIVLGTCTSRIACHVISATVKGQGYSLSADIQYVDSRWEIPVVLPLRDCPA 276

Query: 796 EELSMLCSLD-SLKTVETFQQPHCGINGLISSFVKLLREENPSRESTIVRTAGKLKPFSF 620
           +EL+ LCSLD SLK VE    P  GINGL+SSFVK+L+EENPSRE TIVRTAGKL PF F
Sbjct: 277 QELNTLCSLDGSLKIVELLNGPCSGINGLVSSFVKVLQEENPSRECTIVRTAGKLTPFHF 336

Query: 619 NRIIEVNDCNGHSASQRRQKKYNLKPTESLPPETFCSICHSPLKKSD-TTTLNATGNGEA 443
           NR+ E++D N  SA++R QK+Y LKP  SL  ++FC IC SPLKKS+  ++L + GN + 
Sbjct: 337 NRVPEIHDSNVPSATRRHQKRYTLKPNGSLSSDSFCPICSSPLKKSNFPSSLRSHGN-QQ 395

Query: 442 SVGIFEDTCSSCQYQILPQEPSSLESFRSLLPQQMISLAKNGSCDRQRWLREEMQEFLLS 263
           +  +    CSSCQ+QILP++PS +E F S+LPQQMI+ AK+G       LRE++QEFLLS
Sbjct: 396 NPDLIASACSSCQFQILPKDPSLMEEFLSVLPQQMITKAKHGDQGDFSLLREQIQEFLLS 455

Query: 262 DSED 251
           D E+
Sbjct: 456 DGEN 459


>ref|XP_004306206.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Fragaria vesca
            subsp. vesca]
          Length = 455

 Score =  298 bits (764), Expect = 4e-78
 Identities = 152/255 (59%), Positives = 191/255 (74%), Gaps = 2/255 (0%)
 Frame = -1

Query: 1003 LLHYAVSVVQ-IAVENGYTKLVLGTCTSRIACRVLEATVKGQGYSLAADIQYVDARWEIP 827
            LLH  +  +Q IA ENGY +++LG+C SRIAC V+ ATVKGQGYSL ADIQYVD+RWEIP
Sbjct: 201  LLHLRMLALQKIASENGYNRILLGSCISRIACHVITATVKGQGYSLPADIQYVDSRWEIP 260

Query: 826  VVLPLRDCISEELSMLCSLDSLKTVETFQQPHCGINGLISSFVKLLREENPSRESTIVRT 647
            V+LPLRDC+++EL+MLC L+ LKTVE  + P  GINGL+ SFV LL+EENPSRESTIVRT
Sbjct: 261  VLLPLRDCLAQELNMLCRLEGLKTVELIRSPGTGINGLVLSFVSLLQEENPSRESTIVRT 320

Query: 646  AGKLKPFSFNRIIEVNDCNGHSASQRRQKKYNLKPTESLPPETFCSICHSPLKKSDTTTL 467
            AGKL PF FNRI E++D N   A++RRQK+YNLKP E    E+FC IC+ PL KSD  +L
Sbjct: 321  AGKLIPFHFNRIQEIDDSNVPLATRRRQKRYNLKPNECFSSESFCFICNGPLSKSDLLSL 380

Query: 466  NATGNGEASVGIF-EDTCSSCQYQILPQEPSSLESFRSLLPQQMISLAKNGSCDRQRWLR 290
               G  + S   F    CSSC++QILPQ+PS L+ F + LPQQ+++ AK  + D  R LR
Sbjct: 381  RNLGQCQTSPDTFGAACCSSCRFQILPQDPSLLDQFSADLPQQLVARAKLDNLDNYRMLR 440

Query: 289  EEMQEFLLSDSEDGT 245
            E++Q+ LLS+ ED T
Sbjct: 441  EQIQDCLLSEGEDET 455


>ref|XP_011048047.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Populus
            euphratica]
          Length = 458

 Score =  297 bits (760), Expect = 1e-77
 Identities = 151/254 (59%), Positives = 193/254 (75%), Gaps = 1/254 (0%)
 Frame = -1

Query: 1003 LLHYAVSVVQ-IAVENGYTKLVLGTCTSRIACRVLEATVKGQGYSLAADIQYVDARWEIP 827
            LLH  +  +Q +A +NGY +L+LG+CTSRIAC VL ATVKGQGYSL+ADIQYVDARWEIP
Sbjct: 206  LLHLRMLALQKVASQNGYNRLILGSCTSRIACHVLAATVKGQGYSLSADIQYVDARWEIP 265

Query: 826  VVLPLRDCISEELSMLCSLDSLKTVETFQQPHCGINGLISSFVKLLREENPSRESTIVRT 647
            VVLPLRDC S+EL++LC LD LKT++ F +P  GINGLISSFV +L+EEN SRE TI+RT
Sbjct: 266  VVLPLRDCTSQELNLLCHLDGLKTLQPFDRPPSGINGLISSFVNVLQEENSSRECTILRT 325

Query: 646  AGKLKPFSFNRIIEVNDCNGHSASQRRQKKYNLKPTESLPPETFCSICHSPLKKSDTTTL 467
            AGKL PF FNRI E +DCN   A +RRQK+ NLK  ES+  E FC IC+ PLK  ++  L
Sbjct: 326  AGKLTPFEFNRIPETDDCNVPLAIRRRQKRSNLKTNESIFSELFCPICNGPLKNFNSLNL 385

Query: 466  NATGNGEASVGIFEDTCSSCQYQILPQEPSSLESFRSLLPQQMISLAKNGSCDRQRWLRE 287
            ++  + ++S       CSSCQ+QI+P++PSS+E+F SLLPQQ++  AK+G+C     LRE
Sbjct: 386  SSLKSCQSS-RFNAACCSSCQFQIIPKDPSSMENFCSLLPQQVVDRAKHGNCSNSSLLRE 444

Query: 286  EMQEFLLSDSEDGT 245
            ++Q+ LL DS D T
Sbjct: 445  QIQDCLLLDSGDET 458


>ref|XP_002308030.2| hypothetical protein POPTR_0006s04980g [Populus trichocarpa]
            gi|550335487|gb|EEE91553.2| hypothetical protein
            POPTR_0006s04980g [Populus trichocarpa]
          Length = 458

 Score =  295 bits (756), Expect = 3e-77
 Identities = 150/254 (59%), Positives = 193/254 (75%), Gaps = 1/254 (0%)
 Frame = -1

Query: 1003 LLHYAVSVVQ-IAVENGYTKLVLGTCTSRIACRVLEATVKGQGYSLAADIQYVDARWEIP 827
            LLH  +  +Q +A +NGY +L+LG+CTSRIAC VL ATVKGQGYSL+ADIQYVDARWEIP
Sbjct: 206  LLHLRMLALQMVASQNGYNRLILGSCTSRIACHVLAATVKGQGYSLSADIQYVDARWEIP 265

Query: 826  VVLPLRDCISEELSMLCSLDSLKTVETFQQPHCGINGLISSFVKLLREENPSRESTIVRT 647
            VVLPLRDC S+EL++LC LD LKT++ F     GINGLISSFV +L+EEN SRE TI+RT
Sbjct: 266  VVLPLRDCTSQELNLLCQLDGLKTLQPFDSTPSGINGLISSFVNVLQEENSSRECTILRT 325

Query: 646  AGKLKPFSFNRIIEVNDCNGHSASQRRQKKYNLKPTESLPPETFCSICHSPLKKSDTTTL 467
            AGKL PF FNRI E ++CN   A +RRQK+YNLK  ES+  E FC IC++PLK  ++  L
Sbjct: 326  AGKLTPFEFNRIPETDNCNVPLAIRRRQKRYNLKTKESISSELFCPICNAPLKNFNSLNL 385

Query: 466  NATGNGEASVGIFEDTCSSCQYQILPQEPSSLESFRSLLPQQMISLAKNGSCDRQRWLRE 287
            ++  + ++S       CSSCQ+QI+P++PSS+ +F SLLPQQ++  AK+G+C     LR 
Sbjct: 386  SSLKSCQSS-RFNAACCSSCQFQIIPKDPSSMGNFCSLLPQQVVDRAKHGNCSNLSLLRG 444

Query: 286  EMQEFLLSDSEDGT 245
            ++Q+ LLSDSED T
Sbjct: 445  QIQDCLLSDSEDET 458


>ref|XP_008222118.1| PREDICTED: LOW QUALITY PROTEIN: cytoplasmic tRNA 2-thiolation protein
            2 [Prunus mume]
          Length = 464

 Score =  295 bits (755), Expect = 4e-77
 Identities = 151/255 (59%), Positives = 193/255 (75%), Gaps = 2/255 (0%)
 Frame = -1

Query: 1003 LLHYAVSVVQ-IAVENGYTKLVLGTCTSRIACRVLEATVKGQGYSLAADIQYVDARWEIP 827
            LLH  +  +Q +A +NGY +L+LG+C SRIAC V+ ATVKGQGYSL AD+QYVDARWEIP
Sbjct: 215  LLHLRMLALQXVASQNGYNRLLLGSCVSRIACHVISATVKGQGYSLPADVQYVDARWEIP 274

Query: 826  VVLPLRDCISEELSMLCSLDSLKTVETFQQPHCGINGLISSFVKLLREENPSRESTIVRT 647
            VVLPLRDC+++EL+MLC LD LKTVE  + P  GINGL+SSFV LL+EENPSRE TI+RT
Sbjct: 275  VVLPLRDCLAKELTMLCRLDGLKTVELIRSPCTGINGLVSSFVTLLQEENPSRECTIMRT 334

Query: 646  AGKLKPFSFNRIIEVNDCNGHSASQRRQKKYNLKPTESLPPETFCSICHSPLKKSDTTTL 467
            AGKL PF FN+I E++D N   A++RRQK+YNLKP ES   E+FC IC+SPL +SD  +L
Sbjct: 335  AGKLIPFHFNKIEEIDDSNVLLATRRRQKRYNLKPNESFSSESFCFICNSPLSRSDLLSL 394

Query: 466  -NATGNGEASVGIFEDTCSSCQYQILPQEPSSLESFRSLLPQQMISLAKNGSCDRQRWLR 290
             N   +  +SV      CSSCQ+QILPQ+PSS+    +LLP+Q+ + AK+ + D    LR
Sbjct: 395  KNLESHKTSSV-----CCSSCQFQILPQDPSSVNELYTLLPEQLFARAKHENLDHYSALR 449

Query: 289  EEMQEFLLSDSEDGT 245
            E++Q+ LLS+ ED T
Sbjct: 450  EQIQDCLLSEGEDET 464


>ref|XP_007204337.1| hypothetical protein PRUPE_ppa005400mg [Prunus persica]
            gi|462399868|gb|EMJ05536.1| hypothetical protein
            PRUPE_ppa005400mg [Prunus persica]
          Length = 463

 Score =  294 bits (752), Expect = 9e-77
 Identities = 150/255 (58%), Positives = 193/255 (75%), Gaps = 2/255 (0%)
 Frame = -1

Query: 1003 LLHYAVSVVQ-IAVENGYTKLVLGTCTSRIACRVLEATVKGQGYSLAADIQYVDARWEIP 827
            LLH  +  +Q +A +NGY +L+LG+C SRIAC V+ ATVKGQGYSL AD+QYVDARWEIP
Sbjct: 214  LLHLRMLALQKVASQNGYNRLLLGSCVSRIACHVISATVKGQGYSLPADVQYVDARWEIP 273

Query: 826  VVLPLRDCISEELSMLCSLDSLKTVETFQQPHCGINGLISSFVKLLREENPSRESTIVRT 647
            VVLPLRDC+++EL+MLC LD LKTVE  + P  GINGL+SSFV LL+EENPSRE TI+RT
Sbjct: 274  VVLPLRDCLAKELTMLCRLDGLKTVELIRSPCTGINGLVSSFVTLLQEENPSRECTIMRT 333

Query: 646  AGKLKPFSFNRIIEVNDCNGHSASQRRQKKYNLKPTESLPPETFCSICHSPLKKSDTTTL 467
            AGKL PF FN+I E++D N   A++RRQK+YNLKP ES   E+FC IC+SPL +SD  +L
Sbjct: 334  AGKLIPFHFNKIEEIDDSNVLLATRRRQKRYNLKPNESFSSESFCFICNSPLSRSDLLSL 393

Query: 466  -NATGNGEASVGIFEDTCSSCQYQILPQEPSSLESFRSLLPQQMISLAKNGSCDRQRWLR 290
             N   +  +SV      CSSCQ+QILPQ+PSS+    +LLP+Q+ + AK+ + D    +R
Sbjct: 394  KNLESHKTSSV-----CCSSCQFQILPQDPSSVNELYTLLPEQLFARAKHDNLDHCSAIR 448

Query: 289  EEMQEFLLSDSEDGT 245
            E++Q+ LLS+ ED T
Sbjct: 449  EQIQDCLLSEGEDET 463


>ref|XP_010672851.1| PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Beta vulgaris
            subsp. vulgaris] gi|870863897|gb|KMT15030.1| hypothetical
            protein BVRB_3g061550 [Beta vulgaris subsp. vulgaris]
          Length = 474

 Score =  287 bits (734), Expect = 1e-74
 Identities = 149/261 (57%), Positives = 194/261 (74%), Gaps = 4/261 (1%)
 Frame = -1

Query: 1015 GRHSLLHYA--VSVVQIAVENGYTKLVLGTCTSRIACRVLEATVKGQGYSLAADIQYVDA 842
            G+   L Y   + + ++AVENGYT+LVLG+CTS IA  VL ATVKGQG+SL ADIQYVDA
Sbjct: 214  GKEDALTYLRMLCLQKVAVENGYTQLVLGSCTSTIARHVLSATVKGQGFSLPADIQYVDA 273

Query: 841  RWEIPVVLPLRDCISEELSMLCSLDSLKTVETFQQPHCGINGLISSFVKLLREENPSRES 662
            RW++PV+LPLRDC+++EL++LC  D LKT+     P  GIN L+SSFV LL+EENPSRE 
Sbjct: 274  RWQVPVLLPLRDCLAQELNLLCQRDGLKTLVMVDGPQSGINALVSSFVSLLQEENPSREC 333

Query: 661  TIVRTAGKLKPFSFNRIIEV-NDCNGHSASQRRQKKYNLKPTESLPPETFCSICHSPLKK 485
            TIVRTAGKL PFSFNR+ EV +D     ASQRRQKK+N+K  ES PPE+FCS+C+SPL  
Sbjct: 334  TIVRTAGKLTPFSFNRLPEVDDDPKALLASQRRQKKFNIKSDESTPPESFCSLCNSPLHV 393

Query: 484  SDTTTLNATGNGEASVGIFEDTCSSCQYQILPQEPSSLESFRSLLPQQMISLAK-NGSCD 308
            SD++  + +G  +++       CSSC++QILP +  S++ F +LLPQ ++S A  + S  
Sbjct: 394  SDSSLGSFSGEQQSAESFGATCCSSCRFQILPYDTVSMDQFYALLPQPIMSRATISNSSS 453

Query: 307  RQRWLREEMQEFLLSDSEDGT 245
              R LRE++QEFLLSDSEDG+
Sbjct: 454  NHRLLREKIQEFLLSDSEDGS 474