BLASTX nr result
ID: Forsythia21_contig00007590
seq
BLASTX 2.2.25 [Feb-01-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Forsythia21_contig00007590
(915 letters)
Database: ./nr
69,698,275 sequences; 24,982,196,650 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
ref|XP_009770899.1| PREDICTED: uncharacterized protein LOC104221... 138 5e-30
ref|XP_009631731.1| PREDICTED: uncharacterized serine-rich prote... 138 6e-30
ref|XP_009774319.1| PREDICTED: uncharacterized protein LOC104224... 133 2e-28
gb|AAD02549.1| PGPS/D7 [Petunia x hybrida] 131 7e-28
ref|XP_009599569.1| PREDICTED: uncharacterized protein LOC104095... 130 1e-27
ref|XP_009631729.1| PREDICTED: uncharacterized protein LOC104121... 124 9e-26
ref|XP_009770902.1| PREDICTED: uncharacterized protein LOC104221... 123 2e-25
ref|XP_006339331.1| PREDICTED: uncharacterized protein LOC102590... 123 2e-25
ref|XP_011095483.1| PREDICTED: uncharacterized protein LOC105174... 122 3e-25
ref|XP_004249388.1| PREDICTED: uncharacterized protein LOC101268... 122 3e-25
ref|XP_009757756.1| PREDICTED: uncharacterized protein LOC104210... 114 9e-23
ref|XP_009607505.1| PREDICTED: uncharacterized protein LOC104101... 112 3e-22
ref|XP_006348424.1| PREDICTED: uncharacterized protein LOC102593... 112 3e-22
ref|XP_010315854.1| PREDICTED: uncharacterized protein LOC104645... 104 7e-20
ref|XP_011077915.1| PREDICTED: uncharacterized protein LOC105161... 97 2e-17
ref|XP_012836180.1| PREDICTED: uncharacterized protein LOC105956... 96 3e-17
ref|XP_004228732.1| PREDICTED: pectinesterase inhibitor-like [So... 87 1e-14
ref|XP_006840427.1| PREDICTED: pectinesterase 3 [Amborella trich... 64 2e-07
>ref|XP_009770899.1| PREDICTED: uncharacterized protein LOC104221531 [Nicotiana
sylvestris]
Length = 206
Score = 138 bits (348), Expect = 5e-30
Identities = 68/133 (51%), Positives = 96/133 (72%), Gaps = 2/133 (1%)
Frame = -2
Query: 617 APVAEIGGDVPKDEQAKDSKFLESNMIATADKLEKFISTVVEKRLKDPKTDESDKECLQQ 438
A EI ++P S F+++ M AT K ++FIS V++KRL +P TD K+CL+
Sbjct: 69 ATTFEIPKNIPVISTEDVSIFVKAIMDATMSKTDEFISKVIDKRLAEPNTDIYAKDCLET 128
Query: 437 CQEVYESALDAIQKSVEDVSSGDFFKANVDVSAFSTNIDTCDECFSEM--TDPEFQKFDD 264
C+ V+E A DA++++ EDV +G+ +KANVDVSA STNIDTC+EC + + DPEF+KFD+
Sbjct: 129 CKSVFEDAKDAMKRTEEDVKAGNMYKANVDVSAMSTNIDTCNECATSIYGFDPEFKKFDN 188
Query: 263 WARGVASDCLDKI 225
WA+G+ASDCLDKI
Sbjct: 189 WAQGIASDCLDKI 201
>ref|XP_009631731.1| PREDICTED: uncharacterized serine-rich protein C1E8.05-like
[Nicotiana tomentosiformis]
Length = 213
Score = 138 bits (347), Expect = 6e-30
Identities = 67/134 (50%), Positives = 97/134 (72%), Gaps = 2/134 (1%)
Frame = -2
Query: 605 EIGGDVPKDEQAKDSKFLESNMIATADKLEKFISTVVEKRLKDPKTDESDKECLQQCQEV 426
EI ++P S F+++ M AT K ++FI+ V++KRL +P TD K+CL+ C+ V
Sbjct: 80 EIPKNIPVVSTGDVSIFVKAIMDATMSKTDEFITKVIDKRLAEPNTDIYAKDCLETCKSV 139
Query: 425 YESALDAIQKSVEDVSSGDFFKANVDVSAFSTNIDTCDECFSEM--TDPEFQKFDDWARG 252
+E A DA++++ EDV +G+ +KANVDVSA STNIDTC+EC + + DPEF+KFD+WA+G
Sbjct: 140 FEDAKDAMKRTEEDVKAGNMYKANVDVSAMSTNIDTCNECATSIYGFDPEFKKFDNWAQG 199
Query: 251 VASDCLDKIVKYSN 210
+ASDCLDKI S+
Sbjct: 200 IASDCLDKITGQSS 213
>ref|XP_009774319.1| PREDICTED: uncharacterized protein LOC104224387 [Nicotiana
sylvestris]
Length = 192
Score = 133 bits (334), Expect = 2e-28
Identities = 62/137 (45%), Positives = 93/137 (67%), Gaps = 2/137 (1%)
Frame = -2
Query: 614 PVAEIGGDVPKDEQAKDSKFLESNMIATADKLEKFISTVVEKRLKDPKTDESDKECLQQC 435
P+ D+P F++++M+ T K E+FI V++KRL + K D K+CL+ C
Sbjct: 64 PLVAKASDIPS--------FVKASMVGTMTKTEEFIKNVIDKRLANKKMDSYQKDCLETC 115
Query: 434 QEVYESALDAIQKSVEDVSSGDFFKANVDVSAFSTNIDTCDECFSEM--TDPEFQKFDDW 261
+EVYE A+D++ K+ EDV+ G++FKANVD+SA ++ I+TC +C +EM DP FQKF +W
Sbjct: 116 KEVYEDAMDSMTKATEDVNLGNYFKANVDISAMTSFIETCKDCAAEMYGDDPAFQKFQNW 175
Query: 260 ARGVASDCLDKIVKYSN 210
A G+ASDCLDK+ S+
Sbjct: 176 AEGIASDCLDKVAGASS 192
>gb|AAD02549.1| PGPS/D7 [Petunia x hybrida]
Length = 209
Score = 131 bits (329), Expect = 7e-28
Identities = 62/120 (51%), Positives = 91/120 (75%), Gaps = 2/120 (1%)
Frame = -2
Query: 563 SKFLESNMIATADKLEKFISTVVEKRLKDPKTDESDKECLQQCQEVYESALDAIQKSVED 384
S F+++ M AT K ++FI+ V++KRL DPK D K+CL+ C+ V+E A+DA++++ ED
Sbjct: 90 SIFVKATMDATMAKTDEFITKVIDKRLADPKLDIYAKDCLETCKYVFEDAVDAMKRTEED 149
Query: 383 VSSGDFFKANVDVSAFSTNIDTCDECFSEM--TDPEFQKFDDWARGVASDCLDKIVKYSN 210
V +G+++KANVDVSA STNIDTC+EC + + DP F KF++WA+G+ASDCL KI S+
Sbjct: 150 VKAGNYYKANVDVSAMSTNIDTCNECATSIYGDDPVFTKFENWAQGIASDCLAKITGQSS 209
>ref|XP_009599569.1| PREDICTED: uncharacterized protein LOC104095199 [Nicotiana
tomentosiformis]
Length = 192
Score = 130 bits (327), Expect = 1e-27
Identities = 61/137 (44%), Positives = 92/137 (67%), Gaps = 2/137 (1%)
Frame = -2
Query: 614 PVAEIGGDVPKDEQAKDSKFLESNMIATADKLEKFISTVVEKRLKDPKTDESDKECLQQC 435
P+ D+P ++++M+ T K E+FI V++KRL + K D K+CL+ C
Sbjct: 64 PLVAKASDIPS--------LVKASMVGTMTKTEEFIKNVIDKRLANKKMDSYHKDCLETC 115
Query: 434 QEVYESALDAIQKSVEDVSSGDFFKANVDVSAFSTNIDTCDECFSEM--TDPEFQKFDDW 261
+EVYE A+D++ K+ EDV+ G++FKANVD+SA ++ I+TC +C +EM DP FQKF +W
Sbjct: 116 KEVYEDAMDSMTKATEDVNLGNYFKANVDISAMTSFIETCKDCAAEMYGDDPAFQKFQNW 175
Query: 260 ARGVASDCLDKIVKYSN 210
A G+ASDCLDK+ S+
Sbjct: 176 AEGIASDCLDKVAGASS 192
>ref|XP_009631729.1| PREDICTED: uncharacterized protein LOC104121434 [Nicotiana
tomentosiformis]
Length = 186
Score = 124 bits (311), Expect = 9e-26
Identities = 61/136 (44%), Positives = 91/136 (66%), Gaps = 8/136 (5%)
Frame = -2
Query: 593 DVPKDEQA----KDS--KFLESNMIATADKLEKFISTVVEKRLKDPKTDESDKECLQQCQ 432
++P ++Q KDS +++S M + K E F+ TVVEKRL DP D K+CL C+
Sbjct: 51 EIPTNDQVSLGNKDSVNHYVDSLMEKSVSKTEDFLDTVVEKRLSDPAADPFTKDCLLVCK 110
Query: 431 EVYESALDAIQKSVEDVSSGDFFKANVDVSAFSTNIDTCDECFSEM--TDPEFQKFDDWA 258
EVYE+A+DA++K++EDV G ++ ANVD+SA +T+++TC +C E+ D EF FDDWA
Sbjct: 111 EVYENAVDAMKKTIEDVDRGSYYSANVDLSALTTDLETCMDCIKEIYGDDQEFMSFDDWA 170
Query: 257 RGVASDCLDKIVKYSN 210
+ D L+KIV +S+
Sbjct: 171 EKITEDALEKIVGFSS 186
>ref|XP_009770902.1| PREDICTED: uncharacterized protein LOC104221534 [Nicotiana
sylvestris]
Length = 184
Score = 123 bits (309), Expect = 2e-25
Identities = 61/136 (44%), Positives = 92/136 (67%), Gaps = 8/136 (5%)
Frame = -2
Query: 593 DVPKDEQA----KDS--KFLESNMIATADKLEKFISTVVEKRLKDPKTDESDKECLQQCQ 432
++P ++Q KD+ +++S M T K E FI +VVEKRL DP D K+CL C+
Sbjct: 49 EIPTNDQVSLGNKDAVNHYVDSLMEKTVSKTENFIDSVVEKRLSDPAADPFAKDCLLVCK 108
Query: 431 EVYESALDAIQKSVEDVSSGDFFKANVDVSAFSTNIDTCDECFSEM--TDPEFQKFDDWA 258
EVYE+A+DA++K++EDV+ G ++ ANVD+SA +T+++TC +C E+ D EF FDDWA
Sbjct: 109 EVYENAVDAMKKTIEDVNRGSYYSANVDLSALTTDLETCMDCIKEIYGDDQEFMSFDDWA 168
Query: 257 RGVASDCLDKIVKYSN 210
+ D L+KIV +S+
Sbjct: 169 GKITEDALEKIVGFSS 184
>ref|XP_006339331.1| PREDICTED: uncharacterized protein LOC102590924 [Solanum tuberosum]
Length = 190
Score = 123 bits (308), Expect = 2e-25
Identities = 60/131 (45%), Positives = 90/131 (68%), Gaps = 2/131 (1%)
Frame = -2
Query: 596 GDVPKDEQAKDSKFLESNMIATADKLEKFISTVVEKRLKDPKTDESDKECLQQCQEVYES 417
G+V + + +++S M+ T K E+F++ VVEKRL DP D K+CL C+EVYE+
Sbjct: 60 GNVQLGNKESVNNYVDSLMVTTVTKTEQFLNNVVEKRLIDPSADAFVKDCLLVCKEVYEN 119
Query: 416 ALDAIQKSVEDVSSGDFFKANVDVSAFSTNIDTCDECFSEM--TDPEFQKFDDWARGVAS 243
A+DA++K++ DV SG ++ ANVD+SA ST+++TC +C E+ D EF KFDDWA V
Sbjct: 120 AVDAMKKTMVDVDSGAYYSANVDLSALSTDLETCMDCVKEIYGDDQEFIKFDDWAGKVTV 179
Query: 242 DCLDKIVKYSN 210
D ++KIV +S+
Sbjct: 180 DAMEKIVGFSS 190
>ref|XP_011095483.1| PREDICTED: uncharacterized protein LOC105174925 [Sesamum indicum]
Length = 165
Score = 122 bits (307), Expect = 3e-25
Identities = 60/125 (48%), Positives = 81/125 (64%)
Frame = -2
Query: 590 VPKDEQAKDSKFLESNMIATADKLEKFISTVVEKRLKDPKTDESDKECLQQCQEVYESAL 411
VPK+EQAKD ++L M A ++E+F +T V KRLKDP TD+ K+ L C+E Y+ A+
Sbjct: 45 VPKEEQAKDCQYLTEQMTAAVKQVEQFSATAVAKRLKDPPTDDCAKKALLLCEEAYKKAV 104
Query: 410 DAIQKSVEDVSSGDFFKANVDVSAFSTNIDTCDECFSEMTDPEFQKFDDWARGVASDCLD 231
+ ++K VE VS+GDF +A + F I+ CD+ F EF+ F DWARGV DCL
Sbjct: 105 EMLKKGVESVSAGDFVQAKKNSRTFINYINACDDSFG-----EFRNFGDWARGVGDDCLG 159
Query: 230 KIVKY 216
KIVKY
Sbjct: 160 KIVKY 164
>ref|XP_004249388.1| PREDICTED: uncharacterized protein LOC101268155 [Solanum
lycopersicum]
Length = 190
Score = 122 bits (307), Expect = 3e-25
Identities = 60/130 (46%), Positives = 93/130 (71%), Gaps = 3/130 (2%)
Frame = -2
Query: 605 EIGGDVPKDEQAKD-SKFLESNMIATADKLEKFISTVVEKRLKDPKTDESDKECLQQCQE 429
EI ++P +D S F+ S M AT K E+FI V+E+RL++P TD +CL+ C+
Sbjct: 56 EIPKNIPIVPNTRDVSVFINSMMEATMGKTEEFIVNVIEQRLEEPDTDIYATDCLETCKS 115
Query: 428 VYESALDAIQKSVEDVSSGDFFKANVDVSAFSTNIDTCDECFSEM--TDPEFQKFDDWAR 255
VY+ A+DA++K+ EDV S +++KA++D+SA +T+IDTC+EC + D EF++FD+W +
Sbjct: 116 VYQDAVDAMKKAEEDVKSKEYYKADLDISAITTDIDTCNECAVSIYGEDTEFRQFDNWIQ 175
Query: 254 GVASDCLDKI 225
GVA++CLDKI
Sbjct: 176 GVATECLDKI 185
>ref|XP_009757756.1| PREDICTED: uncharacterized protein LOC104210532 [Nicotiana
sylvestris]
Length = 182
Score = 114 bits (285), Expect = 9e-23
Identities = 57/139 (41%), Positives = 87/139 (62%), Gaps = 2/139 (1%)
Frame = -2
Query: 620 IAPVAEIGGDVPKDEQAKDSKFLESNMIATADKLEKFISTVVEKRLKDPKTDESDKECLQ 441
+ P D A + S M+++ K E F++ VVEKRL D K+CL+
Sbjct: 45 VIPTETADVDAGNSNGASVKNLVGSLMVSSISKTEDFVNKVVEKRLARI-VDAYKKDCLE 103
Query: 440 QCQEVYESALDAIQKSVEDVSSGDFFKANVDVSAFSTNIDTCDECFSEM--TDPEFQKFD 267
C+EVYE A+DA+++++EDV+ G+++KANVDVSA S+N++TC EC + + DPEF KFD
Sbjct: 104 TCKEVYEDAVDAMKETLEDVNEGNYYKANVDVSAMSSNMETCKECVNAIYGQDPEFTKFD 163
Query: 266 DWARGVASDCLDKIVKYSN 210
+WA+ + D L +I S+
Sbjct: 164 NWAQAIIEDALTRITSVSS 182
>ref|XP_009607505.1| PREDICTED: uncharacterized protein LOC104101715 [Nicotiana
tomentosiformis]
Length = 179
Score = 112 bits (281), Expect = 3e-22
Identities = 56/138 (40%), Positives = 85/138 (61%), Gaps = 2/138 (1%)
Frame = -2
Query: 617 APVAEIGGDVPKDEQAKDSKFLESNMIATADKLEKFISTVVEKRLKDPKTDESDKECLQQ 438
+P D A + S M+++ K E F++ VVEKRL D K+CL+
Sbjct: 43 SPTETADVDASNSNGASVKNLVGSLMVSSISKTEDFVNKVVEKRLAKI-VDAYKKDCLET 101
Query: 437 CQEVYESALDAIQKSVEDVSSGDFFKANVDVSAFSTNIDTCDECFSEM--TDPEFQKFDD 264
C+EVYE A+DA+++++EDV+ G+++KANVDVS S+N++TC EC + + DPEF KFD+
Sbjct: 102 CREVYEDAVDAMKETLEDVNEGNYYKANVDVSTMSSNMETCKECVNAIYGQDPEFTKFDN 161
Query: 263 WARGVASDCLDKIVKYSN 210
WA + D L +I S+
Sbjct: 162 WAHAIIEDALTRITSVSS 179
>ref|XP_006348424.1| PREDICTED: uncharacterized protein LOC102593013 [Solanum tuberosum]
Length = 190
Score = 112 bits (281), Expect = 3e-22
Identities = 54/116 (46%), Positives = 80/116 (68%), Gaps = 3/116 (2%)
Frame = -2
Query: 557 FLESNMIATADKLEKFISTVVEKRLK-DPKTDESDKECLQQCQEVYESALDAIQKSVEDV 381
F+++ M+ T K ++FI V+EKRL K D K+CL+ C+EVY A+DA++K+ +DV
Sbjct: 70 FVKATMVGTMSKTQEFIKNVIEKRLALSAKMDHYRKDCLETCKEVYGDAIDAMKKASQDV 129
Query: 380 SSGDFFKANVDVSAFSTNIDTCDECFSEM--TDPEFQKFDDWARGVASDCLDKIVK 219
+ +F+KAN+D+SA + I+TC +C E D FQ F+ WA+GVASDCLDK+ K
Sbjct: 130 KAQNFYKANMDLSAMYSYIETCHDCVVETKENDLAFQNFEKWAKGVASDCLDKVSK 185
>ref|XP_010315854.1| PREDICTED: uncharacterized protein LOC104645662 [Solanum
lycopersicum] gi|511774787|gb|AGN92796.1| petin
methylesterase inhibitor [Solanum lycopersicum]
Length = 184
Score = 104 bits (260), Expect = 7e-20
Identities = 46/116 (39%), Positives = 78/116 (67%), Gaps = 3/116 (2%)
Frame = -2
Query: 557 FLESNMIATADKLEKFISTVVEKRL-KDPKTDESDKECLQQCQEVYESALDAIQKSVEDV 381
F+++++++T K + FI V++KRL D K+C + C+EVYE A+D+++K+ +DV
Sbjct: 64 FVKASLVSTMAKTQDFIKNVIDKRLASSAPIDHYKKDCFKTCKEVYEDAIDSMKKATQDV 123
Query: 380 SSGDFFKANVDVSAFSTNIDTCDECFSEMTD--PEFQKFDDWARGVASDCLDKIVK 219
+++KAN+D+ A T +DTC +C + D P F++FD WA+ +ASDCL K+ K
Sbjct: 124 KELNYYKANMDIGASYTYLDTCRDCIVDTKDNEPAFEQFDRWAKAIASDCLSKVSK 179
>ref|XP_011077915.1| PREDICTED: uncharacterized protein LOC105161799 [Sesamum indicum]
Length = 184
Score = 96.7 bits (239), Expect = 2e-17
Identities = 50/125 (40%), Positives = 74/125 (59%), Gaps = 1/125 (0%)
Frame = -2
Query: 587 PKDEQAKD-SKFLESNMIATADKLEKFISTVVEKRLKDPKTDESDKECLQQCQEVYESAL 411
P++ AKD + +L +M A +E F ST + KRL D TDE K+ L C+E+Y++A+
Sbjct: 60 PEEHAAKDMTHYLTEHMSAAVKMVEDFSSTTLGKRLNDSSTDEYTKKTLLACEEMYKNAV 119
Query: 410 DAIQKSVEDVSSGDFFKANVDVSAFSTNIDTCDECFSEMTDPEFQKFDDWARGVASDCLD 231
A+ + VS+GDF +A + F NI+ CD+ + EFQ F +WAR V DCL
Sbjct: 120 QAMNNGMNSVSTGDFVQARAETHTFINNINACDD-----SSMEFQTFAEWAREVGGDCLA 174
Query: 230 KIVKY 216
KIV++
Sbjct: 175 KIVEH 179
>ref|XP_012836180.1| PREDICTED: uncharacterized protein LOC105956822 [Erythranthe
guttatus]
Length = 236
Score = 95.9 bits (237), Expect = 3e-17
Identities = 49/128 (38%), Positives = 78/128 (60%), Gaps = 6/128 (4%)
Frame = -2
Query: 581 DEQAKDSKFLESNMIATADKLEKFISTVVEKRLKDPKTDES-DKECLQQCQEVYESALDA 405
D++A D FL +++ ATA+++E F+STVV + LK P + +C+ C +Y ALD
Sbjct: 109 DDRAHDIHFLRTHITATANQVELFLSTVVPEMLKGPAAKKQCQPQCVAVCPHIYTDALDD 168
Query: 404 IQKSVEDVSSGDFFKANVDVSAFSTNIDTCDECF--SEMTDP---EFQKFDDWARGVASD 240
++K +E + DF+ NV + A+ T+IDTCD C SE D EF+ FD WA+ + ++
Sbjct: 169 LKKGLESLQKEDFYMLNVRLVAYITDIDTCDHCIIESEKVDDCLVEFRNFDAWAKKIGNE 228
Query: 239 CLDKIVKY 216
L + +KY
Sbjct: 229 LLARSIKY 236
>ref|XP_004228732.1| PREDICTED: pectinesterase inhibitor-like [Solanum lycopersicum]
Length = 156
Score = 87.4 bits (215), Expect = 1e-14
Identities = 45/129 (34%), Positives = 75/129 (58%), Gaps = 3/129 (2%)
Frame = -2
Query: 587 PKDEQAKDSKFLESNMIATADKLEKFISTVVEKRLKDPKTDESDKECLQQCQEVYESALD 408
P ++A + M++T K E+F+ T + RL K + +K+CL C+EVYE A+D
Sbjct: 29 PNVDKANVKNLVTPLMVSTIAKTEEFVKTNIITRLATVK-ENPNKDCLDTCKEVYEDAVD 87
Query: 407 AIQKSVEDVSSGDFFKANVDVSAFSTNIDTCDECFSEM---TDPEFQKFDDWARGVASDC 237
A++K+++ V G++ +A V VSA + + TC + E+ +PE KF+DW+ GV SD
Sbjct: 88 AMKKTIKSVDEGNYVEALVHVSAVGSFMGTCKDSIEEIHCDVNPEMTKFEDWSNGVISDA 147
Query: 236 LDKIVKYSN 210
KI ++
Sbjct: 148 TTKIASVAH 156
>ref|XP_006840427.1| PREDICTED: pectinesterase 3 [Amborella trichopoda]
gi|548842145|gb|ERN02102.1| hypothetical protein
AMTR_s00045p00160800 [Amborella trichopoda]
Length = 236
Score = 63.5 bits (153), Expect = 2e-07
Identities = 42/131 (32%), Positives = 60/131 (45%), Gaps = 2/131 (1%)
Frame = -2
Query: 620 IAPVAEIGGDVPKDEQAKDSKFLESNMIATADKLEKFISTVVEKRLKDPKTDESDKECLQ 441
I P E G +P D + +IA + K ++ K DP T ECLQ
Sbjct: 103 ILPYLEANGGLPLDPATA----FHAEVIACQTEA-KVAKSIAAKMAADPTTSSDTVECLQ 157
Query: 440 QCQEVYESALDAIQKSVEDVSSGDFFKANVDVSAFSTNIDTCDECFSEM--TDPEFQKFD 267
C E Y+ ALD I K+V ++ D N +SA T+ TCD+ F E+ TD +D
Sbjct: 158 ICIENYDDALDNIDKAVAALAKKDLGTVNTMLSAALTDFSTCDDAFGELPATDSPMASYD 217
Query: 266 DWARGVASDCL 234
D ++S+ L
Sbjct: 218 DKLNKLSSNLL 228