BLASTX 2.2.25 [Feb-01-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Forsythia21_contig00003656
         (1875 letters)

Database: ./nr 
           69,698,275 sequences; 24,982,196,650 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

ref|XP_012841498.1| PREDICTED: vacuole membrane protein KMS1 [Er...   713   0.0  
ref|XP_011071573.1| PREDICTED: vacuole membrane protein KMS1-lik...   712   0.0  
ref|XP_011090736.1| PREDICTED: vacuole membrane protein KMS1 [Se...   665   0.0  
emb|CDO99038.1| unnamed protein product [Coffea canephora]            655   0.0  
ref|XP_009609258.1| PREDICTED: vacuole membrane protein 1-like [...   651   0.0  
ref|XP_009800965.1| PREDICTED: vacuole membrane protein 1 [Nicot...   650   0.0  
ref|XP_008224532.1| PREDICTED: vacuole membrane protein 1 [Prunu...   644   0.0  
ref|XP_012481847.1| PREDICTED: vacuole membrane protein KMS1-lik...   640   0.0  
ref|XP_007222446.1| hypothetical protein PRUPE_ppa006162mg [Prun...   638   e-180
ref|XP_012832452.1| PREDICTED: vacuole membrane protein KMS1-lik...   636   e-179
ref|XP_006489364.1| PREDICTED: LOW QUALITY PROTEIN: vacuole memb...   636   e-179
ref|XP_007034717.1| SNARE associated Golgi protein family isofor...   636   e-179
ref|XP_011021552.1| PREDICTED: vacuole membrane protein KMS1 [Po...   632   e-178
ref|XP_002314707.2| hypothetical protein POPTR_0010s09980g [Popu...   631   e-178
gb|KHG15586.1| Vacuole membrane 1 [Gossypium arboreum]                629   e-177
ref|XP_012069605.1| PREDICTED: vacuole membrane protein KMS1 iso...   627   e-177
gb|KDP40157.1| hypothetical protein JCGZ_02155 [Jatropha curcas]      627   e-177
ref|NP_567450.1| ER associated membrane protein KMS1 [Arabidopsi...   627   e-176
ref|XP_002281783.1| PREDICTED: vacuole membrane protein KMS1 [Vi...   625   e-176
gb|AAL24087.1| unknown protein [Arabidopsis thaliana]                 625   e-176

>ref|XP_012841498.1| PREDICTED: vacuole membrane protein KMS1 [Erythranthe guttatus]
            gi|604347394|gb|EYU45646.1| hypothetical protein
            MIMGU_mgv1a006701mg [Erythranthe guttata]
          Length = 434

 Score =  713 bits (1841), Expect = 0.0
 Identities = 346/424 (81%), Positives = 386/424 (91%), Gaps = 1/424 (0%)
 Frame = -2

Query: 1577 NSPSGSEDRDMSISGLHWKHQQELENLTLTSQPIKTLKLCTLAIIQYLRQSVAYLLSHGG 1398
            NS   S +R MSISGL  KHQQELENL+LTSQP KTLKL  LA++QYLR+S AYLLSHGG
Sbjct: 8    NSNKSSRNRGMSISGLRLKHQQELENLSLTSQPFKTLKLFVLAVVQYLRRSFAYLLSHGG 67

Query: 1397 WLMLLSTMIVIAGVF-VTTNGPHEKHVEEVLRYVRFGLWWMALGVASSIGLGSGLHTFVL 1221
            WL++LS ++ IAG+  VT +GPHEKHVEEVL Y+RFGLWWMALGVASSIGLGSGLHTFVL
Sbjct: 68   WLIVLSALVAIAGILLVTVDGPHEKHVEEVLHYMRFGLWWMALGVASSIGLGSGLHTFVL 127

Query: 1220 YLGPHIALFTIKAMQCGRVDIKSAPYDTIQLKRPPSWLGKDCAEFGPPLFSSSHGTQVPL 1041
            YLGPHIALFT+KA+QCGRVDIKSA YDTIQLKR PSWLGKDC EFGPPLF SSHG ++PL
Sbjct: 128  YLGPHIALFTLKAVQCGRVDIKSALYDTIQLKRAPSWLGKDCDEFGPPLFPSSHGVRIPL 187

Query: 1040 SAILPQVQLEAILWGLGTALGELPPYFISRAASLSGGKLDAMEELDASSTEDNGFMAARL 861
            S++LPQVQ+EAILWGLGTALGELPPYFISRAASLSGGK+DAMEELDASSTED GF+++RL
Sbjct: 188  SSLLPQVQIEAILWGLGTALGELPPYFISRAASLSGGKVDAMEELDASSTEDTGFLSSRL 247

Query: 860  TQIKRWFLSHAQYLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKAIIKT 681
             +IKR FLSHAQYLNFFTILVLAS+PNPLFDLAGIMCGQFGIPFW+FF+ATMIGKAIIKT
Sbjct: 248  NRIKRSFLSHAQYLNFFTILVLASIPNPLFDLAGIMCGQFGIPFWKFFVATMIGKAIIKT 307

Query: 680  HIQTLFIISVCNNQLLDWVENELIWMLSFVPGFDSILPNIVAKLHSMKDKYLATKSRVPL 501
            HIQT+FIISVCNNQLL W+ENELIW+LSF+PGFDSILPN+V+KLHSMKDKYLATK  VP+
Sbjct: 308  HIQTVFIISVCNNQLLHWMENELIWVLSFIPGFDSILPNLVSKLHSMKDKYLATKHHVPI 367

Query: 500  NVKAKKWDFSLAFIWNTVVWFMLMNFFVKIVNATAQRYAKKNQEKELAALENQS*EHSRD 321
            N K KKWDFSLAFIWNTVVWFMLMNFFVKIVNATA+RY KK  +KE+AAL+N+S   S+ 
Sbjct: 368  NTKGKKWDFSLAFIWNTVVWFMLMNFFVKIVNATAERYLKKQHDKEIAALKNKS---SKQ 424

Query: 320  SDHS 309
            SD S
Sbjct: 425  SDES 428


>ref|XP_011071573.1| PREDICTED: vacuole membrane protein KMS1-like [Sesamum indicum]
          Length = 433

 Score =  712 bits (1838), Expect = 0.0
 Identities = 349/426 (81%), Positives = 383/426 (89%), Gaps = 1/426 (0%)
 Frame = -2

Query: 1577 NSPSGSEDRDMSISGLHWKHQQELENLTLTSQPIKTLKLCTLAIIQYLRQSVAYLLSHGG 1398
            +S      R MSISGL  KHQQELENLTLTSQP KTLKL  LA+ +YL +SVAYLLSH G
Sbjct: 8    SSSKNHRSRGMSISGLRIKHQQELENLTLTSQPFKTLKLFILAVTRYLSRSVAYLLSHRG 67

Query: 1397 WLMLLSTMIVIAGVF-VTTNGPHEKHVEEVLRYVRFGLWWMALGVASSIGLGSGLHTFVL 1221
            WLMLLST++ IAGV  VT +GPHEKHVEEVLRY+RFGLWW+ALGVASSIGLGSGLHTFVL
Sbjct: 68   WLMLLSTIVAIAGVLLVTVDGPHEKHVEEVLRYLRFGLWWVALGVASSIGLGSGLHTFVL 127

Query: 1220 YLGPHIALFTIKAMQCGRVDIKSAPYDTIQLKRPPSWLGKDCAEFGPPLFSSSHGTQVPL 1041
            YLGPHIALFTIKA+QCGRVDIKSA YDTIQLKR PSWLGKDCAEFGPPLF SSHG ++PL
Sbjct: 128  YLGPHIALFTIKAVQCGRVDIKSALYDTIQLKRVPSWLGKDCAEFGPPLFPSSHGVRIPL 187

Query: 1040 SAILPQVQLEAILWGLGTALGELPPYFISRAASLSGGKLDAMEELDASSTEDNGFMAARL 861
            S+ILPQVQLEAILWGLGTALGELPPYFISRAA LSGGK++AMEELD SS ED GF++ARL
Sbjct: 188  SSILPQVQLEAILWGLGTALGELPPYFISRAARLSGGKVNAMEELDDSSKEDEGFISARL 247

Query: 860  TQIKRWFLSHAQYLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKAIIKT 681
             +IKRWFLSHAQYLNFFTIL+LASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKAIIKT
Sbjct: 248  NKIKRWFLSHAQYLNFFTILILASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKAIIKT 307

Query: 680  HIQTLFIISVCNNQLLDWVENELIWMLSFVPGFDSILPNIVAKLHSMKDKYLATKSRVPL 501
            HIQT+FIISVCNNQLL W+ENELIW+LSF+PGFDSILPN+V+KLH +KDKY+ATK  VP 
Sbjct: 308  HIQTVFIISVCNNQLLHWMENELIWVLSFIPGFDSILPNLVSKLHKIKDKYMATKHHVPA 367

Query: 500  NVKAKKWDFSLAFIWNTVVWFMLMNFFVKIVNATAQRYAKKNQEKELAALENQS*EHSRD 321
            N K  KW+FSLAFIWNT+VWFMLMNFFVKIVNATAQRY KK Q+KE+AAL+N+S + S D
Sbjct: 368  NNKGNKWEFSLAFIWNTIVWFMLMNFFVKIVNATAQRYLKKQQDKEIAALKNKSSKQSDD 427

Query: 320  SDHSPR 303
            SD S R
Sbjct: 428  SDTSSR 433


>ref|XP_011090736.1| PREDICTED: vacuole membrane protein KMS1 [Sesamum indicum]
          Length = 431

 Score =  665 bits (1717), Expect = 0.0
 Identities = 327/419 (78%), Positives = 372/419 (88%), Gaps = 1/419 (0%)
 Frame = -2

Query: 1562 SEDRDMSISGLHWKHQQELENLTLTSQPIKTLKLCTLAIIQYLRQSVAYLLSHGGWLMLL 1383
            S DR MSISGL  +HQQELE LTLTSQP+KTLKL  LA+  YLRQS AYLLSHGGWLMLL
Sbjct: 13   SRDRAMSISGLQIRHQQELEKLTLTSQPLKTLKLFCLAVRLYLRQSAAYLLSHGGWLMLL 72

Query: 1382 STMIVIAGVF-VTTNGPHEKHVEEVLRYVRFGLWWMALGVASSIGLGSGLHTFVLYLGPH 1206
            ST++VI GV  VT +GPHEKHVEEV +Y++F LWW+ALGVASSIGLGSGLHTFVLYLGPH
Sbjct: 73   STIVVICGVLLVTVDGPHEKHVEEVQQYIQFALWWVALGVASSIGLGSGLHTFVLYLGPH 132

Query: 1205 IALFTIKAMQCGRVDIKSAPYDTIQLKRPPSWLGKDCAEFGPPLFSSSHGTQVPLSAILP 1026
            IALFTIKA+QCGR DIK+APYDTIQLKR PSWLGKDCA+FG PLF +SHG +VPLS+ILP
Sbjct: 133  IALFTIKAIQCGRADIKTAPYDTIQLKRGPSWLGKDCADFGLPLFQTSHGERVPLSSILP 192

Query: 1025 QVQLEAILWGLGTALGELPPYFISRAASLSGGKLDAMEELDASSTEDNGFMAARLTQIKR 846
            QVQLEAILWGLGTALGELPPYFISRAA LSGG +D ME LDASS +D+GF++ARL QIKR
Sbjct: 193  QVQLEAILWGLGTALGELPPYFISRAARLSGGTVDTME-LDASS-DDDGFISARLNQIKR 250

Query: 845  WFLSHAQYLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKAIIKTHIQTL 666
            WFL HAQ+LNFFTILVLASVPNPLFDLAGIMCGQFGIPFW FFLAT IGKAIIKTHIQT+
Sbjct: 251  WFLLHAQHLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWNFFLATAIGKAIIKTHIQTV 310

Query: 665  FIISVCNNQLLDWVENELIWMLSFVPGFDSILPNIVAKLHSMKDKYLATKSRVPLNVKAK 486
            FII VCNNQLLDW+ENEL+ ++ F PGF+SI+PN+V+KL SMKDKYLATK  VP N++  
Sbjct: 311  FIICVCNNQLLDWMENELLLVMGFAPGFNSIIPNLVSKLQSMKDKYLATKPHVPSNIEVS 370

Query: 485  KWDFSLAFIWNTVVWFMLMNFFVKIVNATAQRYAKKNQEKELAALENQS*EHSRDSDHS 309
            KWDFSLA IWNTV+W MLMNF VKIVN+TAQ+Y KK Q++E+AAL+N+S +HS ++D+S
Sbjct: 371  KWDFSLASIWNTVIWIMLMNFSVKIVNSTAQQYLKKQQDREIAALKNKSSQHSDEADNS 429


>emb|CDO99038.1| unnamed protein product [Coffea canephora]
          Length = 426

 Score =  655 bits (1691), Expect = 0.0
 Identities = 315/395 (79%), Positives = 356/395 (90%), Gaps = 1/395 (0%)
 Frame = -2

Query: 1523 KHQQELENLTLTSQPIKTLKLCTLAIIQYLRQSVAYLLSHGGWLMLLSTMIVIAGVF-VT 1347
            +HQ+ELENLTL +QP KT+KL  LA+IQYL +S AYLL+H GW MLLS + + AG+  VT
Sbjct: 33   RHQEELENLTLITQPFKTIKLFILAVIQYLWRSAAYLLAHAGWFMLLSALFLFAGILLVT 92

Query: 1346 TNGPHEKHVEEVLRYVRFGLWWMALGVASSIGLGSGLHTFVLYLGPHIALFTIKAMQCGR 1167
             +GPHEKHVEEVL Y+RFGLWW+ALGVASSIGLGSGLHTFVLYLGPHIALFTIKA+QCGR
Sbjct: 93   IDGPHEKHVEEVLNYLRFGLWWVALGVASSIGLGSGLHTFVLYLGPHIALFTIKAVQCGR 152

Query: 1166 VDIKSAPYDTIQLKRPPSWLGKDCAEFGPPLFSSSHGTQVPLSAILPQVQLEAILWGLGT 987
            VD+KSAPYDTIQLKR PSWLGKDC+EFGPP+F S HG++VPLS+IL QVQLEAILWGLGT
Sbjct: 153  VDVKSAPYDTIQLKRGPSWLGKDCSEFGPPVFLSLHGSRVPLSSILLQVQLEAILWGLGT 212

Query: 986  ALGELPPYFISRAASLSGGKLDAMEELDASSTEDNGFMAARLTQIKRWFLSHAQYLNFFT 807
            ALGELPPYFISRAAS+SGGK+DAMEELD +STED G +A +L  +KRWFLSHAQYLNFFT
Sbjct: 213  ALGELPPYFISRAASISGGKIDAMEELD-TSTEDRGMLARQLNILKRWFLSHAQYLNFFT 271

Query: 806  ILVLASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKAIIKTHIQTLFIISVCNNQLLDW 627
            IL+LASVPNPLFDLAGIMCGQFGIPFWEFFLAT++GKAIIKTHIQT+FII VCNNQLLDW
Sbjct: 272  ILLLASVPNPLFDLAGIMCGQFGIPFWEFFLATLVGKAIIKTHIQTVFIILVCNNQLLDW 331

Query: 626  VENELIWMLSFVPGFDSILPNIVAKLHSMKDKYLATKSRVPLNVKAKKWDFSLAFIWNTV 447
            +ENELIW+LSFVPGF SILP++++KLH+MKDKYLATK  V  N K  KWDFS A IWNTV
Sbjct: 332  MENELIWVLSFVPGFKSILPDVISKLHAMKDKYLATKPPVSSNFKVNKWDFSFASIWNTV 391

Query: 446  VWFMLMNFFVKIVNATAQRYAKKNQEKELAALENQ 342
            VWFMLMNFF+KIVNATAQRY KK QEK +AAL+N+
Sbjct: 392  VWFMLMNFFIKIVNATAQRYLKKEQEKHIAALKNK 426


>ref|XP_009609258.1| PREDICTED: vacuole membrane protein 1-like [Nicotiana
            tomentosiformis]
          Length = 424

 Score =  651 bits (1680), Expect = 0.0
 Identities = 319/411 (77%), Positives = 363/411 (88%), Gaps = 1/411 (0%)
 Frame = -2

Query: 1568 SGSEDRDMSISGLHWKHQQELENLTLTSQPIKTLKLCTLAIIQYLRQSVAYLLSHGGWLM 1389
            S S  ++M +S L  KHQQ+LENL L +QP +TLKL ++A++QYLR+S+AYLLSH  W M
Sbjct: 15   SASPVKEMPVSDLRAKHQQDLENLNLLTQPFRTLKLFSMAMMQYLRKSIAYLLSHR-WCM 73

Query: 1388 LLSTMIVIA-GVFVTTNGPHEKHVEEVLRYVRFGLWWMALGVASSIGLGSGLHTFVLYLG 1212
            L ST+ V+A  + VTT+GPH KHVEEVLRY++FGLWW+ALGVASSIGLGSGLHTFVLYLG
Sbjct: 74   LFSTIAVLALTLLVTTDGPHVKHVEEVLRYLKFGLWWVALGVASSIGLGSGLHTFVLYLG 133

Query: 1211 PHIALFTIKAMQCGRVDIKSAPYDTIQLKRPPSWLGKDCAEFGPPLFSSSHGTQVPLSAI 1032
            PHIALFTIKAM+CGRVD+K APYDTIQ +R P WL KDC+EFGPP+FSSSHGT VPLS+I
Sbjct: 134  PHIALFTIKAMKCGRVDLKMAPYDTIQFQRTPLWLRKDCSEFGPPVFSSSHGTSVPLSSI 193

Query: 1031 LPQVQLEAILWGLGTALGELPPYFISRAASLSGGKLDAMEELDASSTEDNGFMAARLTQI 852
            LPQVQLEAILWGLGTALGELPPYFISRAASLSG ++DAMEELDASS E NGF+A RLTQ+
Sbjct: 194  LPQVQLEAILWGLGTALGELPPYFISRAASLSGSRVDAMEELDASS-ESNGFLATRLTQM 252

Query: 851  KRWFLSHAQYLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKAIIKTHIQ 672
            KRWFLSHAQYLNFFTIL+LASVPNPLFDLAGIMCGQFGIPFWEFF AT+IGKA+IKTHIQ
Sbjct: 253  KRWFLSHAQYLNFFTILILASVPNPLFDLAGIMCGQFGIPFWEFFFATLIGKALIKTHIQ 312

Query: 671  TLFIISVCNNQLLDWVENELIWMLSFVPGFDSILPNIVAKLHSMKDKYLATKSRVPLNVK 492
            TLFIISVCNNQLLDWVE ELI MLSF+PGF+++LPNI+AKLHSMKDKY+ATK+ V  N+K
Sbjct: 313  TLFIISVCNNQLLDWVETELIRMLSFIPGFETMLPNIIAKLHSMKDKYMATKTPVTSNIK 372

Query: 491  AKKWDFSLAFIWNTVVWFMLMNFFVKIVNATAQRYAKKNQEKELAALENQS 339
              KWDFS A +WN VV  ML+NFFVKIVNATAQRY KK QEKE   L+N+S
Sbjct: 373  VNKWDFSFASVWNGVVTIMLLNFFVKIVNATAQRYLKKQQEKEFTGLKNKS 423


>ref|XP_009800965.1| PREDICTED: vacuole membrane protein 1 [Nicotiana sylvestris]
          Length = 424

 Score =  650 bits (1676), Expect = 0.0
 Identities = 320/411 (77%), Positives = 363/411 (88%), Gaps = 1/411 (0%)
 Frame = -2

Query: 1568 SGSEDRDMSISGLHWKHQQELENLTLTSQPIKTLKLCTLAIIQYLRQSVAYLLSHGGWLM 1389
            S S  ++MSIS L  KHQ++LENL L +QP +TLKL ++A++QY R+S+AYLLSH  W M
Sbjct: 15   SPSPVKEMSISDLRAKHQKDLENLNLLTQPFRTLKLFSMAMMQYFRKSIAYLLSHR-WCM 73

Query: 1388 LLSTMIVIAG-VFVTTNGPHEKHVEEVLRYVRFGLWWMALGVASSIGLGSGLHTFVLYLG 1212
            L ST+ V+A  V VTT+GPH KHVEEVLRY+RFGLWW+ALGVASSIGLGSGLHTFVLYLG
Sbjct: 74   LFSTIAVLALIVLVTTDGPHVKHVEEVLRYLRFGLWWVALGVASSIGLGSGLHTFVLYLG 133

Query: 1211 PHIALFTIKAMQCGRVDIKSAPYDTIQLKRPPSWLGKDCAEFGPPLFSSSHGTQVPLSAI 1032
            PHIALFTIKAM+CGRVD+K APYDTIQ +R P WL KDC+EFGPP+FSSSHGT VPLS+I
Sbjct: 134  PHIALFTIKAMKCGRVDLKMAPYDTIQFQRTPLWLRKDCSEFGPPVFSSSHGTSVPLSSI 193

Query: 1031 LPQVQLEAILWGLGTALGELPPYFISRAASLSGGKLDAMEELDASSTEDNGFMAARLTQI 852
            LPQVQLEAILWGLGTALGELPPYFISRAASLSG ++DAM+ELDASS E NGF+A RLTQ+
Sbjct: 194  LPQVQLEAILWGLGTALGELPPYFISRAASLSGSRVDAMKELDASS-ESNGFLATRLTQM 252

Query: 851  KRWFLSHAQYLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKAIIKTHIQ 672
            KRWFLSHAQYLNFFTIL+LASVPNPLFDLAGIMCGQFGIPFWEFF AT+IGKA+IKTHIQ
Sbjct: 253  KRWFLSHAQYLNFFTILILASVPNPLFDLAGIMCGQFGIPFWEFFFATLIGKALIKTHIQ 312

Query: 671  TLFIISVCNNQLLDWVENELIWMLSFVPGFDSILPNIVAKLHSMKDKYLATKSRVPLNVK 492
            TLFIISVCNNQLLDWVE ELI MLSF+PGF+++LPNI+AKLHSMKDKY+ATK+ V  N+K
Sbjct: 313  TLFIISVCNNQLLDWVETELIRMLSFIPGFETMLPNIIAKLHSMKDKYMATKTPVTSNIK 372

Query: 491  AKKWDFSLAFIWNTVVWFMLMNFFVKIVNATAQRYAKKNQEKELAALENQS 339
              KWDFS A +WN VV  ML+NFFVKIVNATAQRY KK QEKE   L+N+S
Sbjct: 373  VNKWDFSFATVWNGVVTIMLLNFFVKIVNATAQRYLKKQQEKEFTGLKNKS 423


>ref|XP_008224532.1| PREDICTED: vacuole membrane protein 1 [Prunus mume]
          Length = 425

 Score =  644 bits (1661), Expect = 0.0
 Identities = 308/416 (74%), Positives = 361/416 (86%), Gaps = 1/416 (0%)
 Frame = -2

Query: 1583 NENSPSGSEDRDMSISGLHWKHQQELENLTLTSQPIKTLKLCTLAIIQYLRQSVAYLLSH 1404
            + +SPS S+  ++SISGL  KHQ+ELENLTLT+QP +TLK   LA+IQY+++S++YLL+ 
Sbjct: 11   SSSSPSSSQRTEISISGLSEKHQEELENLTLTTQPFRTLKFFILAVIQYVKRSISYLLAK 70

Query: 1403 GGWLMLLSTMIVIAGVF-VTTNGPHEKHVEEVLRYVRFGLWWMALGVASSIGLGSGLHTF 1227
            GGWLMLLST++   GV  VT +GPHEKHVEE+L+Y +FGLWW+ALGVASSIGLGSGLHTF
Sbjct: 71   GGWLMLLSTIVASFGVLLVTIDGPHEKHVEELLKYFQFGLWWVALGVASSIGLGSGLHTF 130

Query: 1226 VLYLGPHIALFTIKAMQCGRVDIKSAPYDTIQLKRPPSWLGKDCAEFGPPLFSSSHGTQV 1047
            VLYLGPHIA FT+KAMQCGRVD+KSAPYDTIQLKR PSWLGKDC+EFGPPLF S HG +V
Sbjct: 131  VLYLGPHIAFFTLKAMQCGRVDLKSAPYDTIQLKRGPSWLGKDCSEFGPPLFPSLHGLRV 190

Query: 1046 PLSAILPQVQLEAILWGLGTALGELPPYFISRAASLSGGKLDAMEELDASSTEDNGFMAA 867
            PLS+ILPQVQLEA+LWG+GTA+GELPPYFISRAASLSG K+D MEELDA S E  GF+A 
Sbjct: 191  PLSSILPQVQLEAVLWGVGTAIGELPPYFISRAASLSGNKVDGMEELDAPSDEGTGFIAT 250

Query: 866  RLTQIKRWFLSHAQYLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKAII 687
             L ++KRW LSH+Q LNFFTILVLASVPNPLFDLAGIMCGQFGIPFW+FFLAT+IGKAII
Sbjct: 251  HLNRVKRWLLSHSQQLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWKFFLATVIGKAII 310

Query: 686  KTHIQTLFIISVCNNQLLDWVENELIWMLSFVPGFDSILPNIVAKLHSMKDKYLATKSRV 507
            KTH+QT+FIISVCNNQLL+W+ENELIW+ S +PGF ++LPN++AK+ + K+KYL T S V
Sbjct: 311  KTHLQTVFIISVCNNQLLNWIENELIWVFSLIPGFAAVLPNLIAKIDAAKEKYL-TASSV 369

Query: 506  PLNVKAKKWDFSLAFIWNTVVWFMLMNFFVKIVNATAQRYAKKNQEKELAALENQS 339
              N+K K WDFS A IWNT+VW ML NFFVKIVNATAQRY KK QEKELA L  +S
Sbjct: 370  SSNIKVKTWDFSFASIWNTLVWLMLFNFFVKIVNATAQRYLKKQQEKELALLTKKS 425


>ref|XP_012481847.1| PREDICTED: vacuole membrane protein KMS1-like [Gossypium raimondii]
            gi|763760959|gb|KJB28213.1| hypothetical protein
            B456_005G041600 [Gossypium raimondii]
          Length = 423

 Score =  640 bits (1652), Expect = 0.0
 Identities = 304/411 (73%), Positives = 359/411 (87%), Gaps = 1/411 (0%)
 Frame = -2

Query: 1580 ENSPSGSEDRDMSISGLHWKHQQELENLTLTSQPIKTLKLCTLAIIQYLRQSVAYLLSHG 1401
            + + S ++  + S+  L  +++ ELENLTLT+QP+KTL+   LAI+QY+++S +YLL+ G
Sbjct: 5    KGATSSAQQMESSVQDLRERYRLELENLTLTTQPVKTLRFFLLAILQYIKRSASYLLAKG 64

Query: 1400 GWLMLLSTMIVIAGVF-VTTNGPHEKHVEEVLRYVRFGLWWMALGVASSIGLGSGLHTFV 1224
            GWLML ST+I + G+  VT  GPHEKHVEEV +YVRFGLWW+ALGVASSIGLGSGLHTFV
Sbjct: 65   GWLMLSSTLIAVLGILLVTIEGPHEKHVEEVSQYVRFGLWWIALGVASSIGLGSGLHTFV 124

Query: 1223 LYLGPHIALFTIKAMQCGRVDIKSAPYDTIQLKRPPSWLGKDCAEFGPPLFSSSHGTQVP 1044
            LYLGPHIALFTIKAMQCGR+D+KSAPYDTIQLKR PSWL K C EFGPPLFSSSHG++VP
Sbjct: 125  LYLGPHIALFTIKAMQCGRIDLKSAPYDTIQLKRVPSWLDKPCREFGPPLFSSSHGSRVP 184

Query: 1043 LSAILPQVQLEAILWGLGTALGELPPYFISRAASLSGGKLDAMEELDASSTEDNGFMAAR 864
            +S+ILPQVQ+EAILWGLGTALGELPPYFISRAAS+SG K++AMEELD SS+EDNGF+A  
Sbjct: 185  ISSILPQVQIEAILWGLGTALGELPPYFISRAASVSGRKIEAMEELDHSSSEDNGFIATH 244

Query: 863  LTQIKRWFLSHAQYLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKAIIK 684
            L QI+ W LSH+Q++NFFTIL+LAS+PNPLFDLAGIMCGQFGIPFW+FFLAT+IGKAIIK
Sbjct: 245  LKQIEHWLLSHSQHMNFFTILLLASIPNPLFDLAGIMCGQFGIPFWKFFLATLIGKAIIK 304

Query: 683  THIQTLFIISVCNNQLLDWVENELIWMLSFVPGFDSILPNIVAKLHSMKDKYLATKSRVP 504
            THIQT+FIISVCNNQLLDW+ENELIW+LSF+PGFDS LP + AKLHS+K+KYLA    VP
Sbjct: 305  THIQTVFIISVCNNQLLDWIENELIWILSFIPGFDSYLPTLTAKLHSVKEKYLAAPPPVP 364

Query: 503  LNVKAKKWDFSLAFIWNTVVWFMLMNFFVKIVNATAQRYAKKNQEKELAAL 351
             N+K  KWDFS A IWNT+VW MLMNFFVKIVNATAQ+Y KK Q+K LA +
Sbjct: 365  SNIK-DKWDFSFASIWNTIVWLMLMNFFVKIVNATAQKYLKKQQDKSLAEI 414


>ref|XP_007222446.1| hypothetical protein PRUPE_ppa006162mg [Prunus persica]
            gi|462419382|gb|EMJ23645.1| hypothetical protein
            PRUPE_ppa006162mg [Prunus persica]
          Length = 425

 Score =  638 bits (1646), Expect = e-180
 Identities = 304/416 (73%), Positives = 359/416 (86%), Gaps = 1/416 (0%)
 Frame = -2

Query: 1583 NENSPSGSEDRDMSISGLHWKHQQELENLTLTSQPIKTLKLCTLAIIQYLRQSVAYLLSH 1404
            + + PS S+  ++SISGL  KHQ+ELENLTLT+QP +TLK   LA++QY+++S++YLL+ 
Sbjct: 11   SSSPPSSSQRTEISISGLSEKHQEELENLTLTTQPFRTLKFFILAVVQYVKRSISYLLAK 70

Query: 1403 GGWLMLLSTMIVIAGVF-VTTNGPHEKHVEEVLRYVRFGLWWMALGVASSIGLGSGLHTF 1227
            GGWLMLLST++   G+  VT +GPHEKHVEE+L+Y +FGLWW+ALGVASSIGLGSGLHTF
Sbjct: 71   GGWLMLLSTVVASLGILLVTIDGPHEKHVEELLKYFQFGLWWVALGVASSIGLGSGLHTF 130

Query: 1226 VLYLGPHIALFTIKAMQCGRVDIKSAPYDTIQLKRPPSWLGKDCAEFGPPLFSSSHGTQV 1047
            VLYLGPHIA FT+KAMQCGRVD+KSAPYDTIQLKR PSWLGK+C+EFGPPLF S HG +V
Sbjct: 131  VLYLGPHIAFFTLKAMQCGRVDLKSAPYDTIQLKRGPSWLGKNCSEFGPPLFPSLHGLRV 190

Query: 1046 PLSAILPQVQLEAILWGLGTALGELPPYFISRAASLSGGKLDAMEELDASSTEDNGFMAA 867
            PLS+ILPQVQLEAILWG+GTA+GELPPYFISRAASLSG K+D MEELDA S E  GF+A 
Sbjct: 191  PLSSILPQVQLEAILWGVGTAIGELPPYFISRAASLSGDKVDGMEELDAPSDEGTGFIAT 250

Query: 866  RLTQIKRWFLSHAQYLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKAII 687
             L ++KRW LSH+Q  NFFTILVLASVPNPLFDLAGIMCGQFGIPFW+FFLAT+IGKAII
Sbjct: 251  HLNRVKRWLLSHSQQFNFFTILVLASVPNPLFDLAGIMCGQFGIPFWKFFLATVIGKAII 310

Query: 686  KTHIQTLFIISVCNNQLLDWVENELIWMLSFVPGFDSILPNIVAKLHSMKDKYLATKSRV 507
            KTH+QT+FIISVCNNQLL+W+ENELIW+ S +PGF ++LPN++AK+ + K+KYL T S V
Sbjct: 311  KTHLQTVFIISVCNNQLLNWIENELIWVFSLIPGFAAVLPNLIAKIDAAKEKYL-TASSV 369

Query: 506  PLNVKAKKWDFSLAFIWNTVVWFMLMNFFVKIVNATAQRYAKKNQEKELAALENQS 339
              N+K K WDFS A IWNT+VW ML NFFVKIVNATAQRY KK QEKELA L  +S
Sbjct: 370  SSNIKVKTWDFSFASIWNTLVWLMLFNFFVKIVNATAQRYLKKQQEKELALLTKKS 425


>ref|XP_012832452.1| PREDICTED: vacuole membrane protein KMS1-like [Erythranthe guttatus]
          Length = 431

 Score =  636 bits (1641), Expect = e-179
 Identities = 309/415 (74%), Positives = 362/415 (87%), Gaps = 2/415 (0%)
 Frame = -2

Query: 1577 NSPSGSEDRDMSISGLHWKHQQELENLTLTSQPIKTLKLCTLAIIQYLRQSVAYLLSHGG 1398
            NS S S D  MSISGLH KH++ELENLTL+SQP +TLKL  LA+ +YL +SV YLLSH  
Sbjct: 8    NSDSISGDIAMSISGLHTKHRRELENLTLSSQPFQTLKLFILAVREYLGKSVVYLLSHRN 67

Query: 1397 WLMLLSTMIVIA-GVFVTTNGPHEKHVEEVLRYVRFGLWWMALGVASSIGLGSGLHTFVL 1221
            W + LS++I+I   + +T +GPHEKHVEEV+ YVRFGLWW+ALGVASSIGLGSGLHTFVL
Sbjct: 68   WFIPLSSIILITCTLLLTVDGPHEKHVEEVIHYVRFGLWWVALGVASSIGLGSGLHTFVL 127

Query: 1220 YLGPHIALFTIKAMQCGRVDIKSAPYDTIQLKRPPSWLGKDCAEFGPPLFSSSHGTQVPL 1041
            YLGPHIALFTIKA+QCGR DIKSAPYDTIQLK  P+WLGKDCAEFGPP F SS+G ++PL
Sbjct: 128  YLGPHIALFTIKAIQCGRTDIKSAPYDTIQLKAGPTWLGKDCAEFGPPFFESSNGVKIPL 187

Query: 1040 SAILPQVQLEAILWGLGTALGELPPYFISRAASLSGGKLDAMEELDASSTEDNGFMAARL 861
            S+ILPQVQLEAILWGLGTALGELPPYFISRAA LSG  ++AMEELD SS E++G ++++L
Sbjct: 188  SSILPQVQLEAILWGLGTALGELPPYFISRAARLSGSTVNAMEELDGSSAENDGRISSQL 247

Query: 860  TQIKRWFLSHAQYLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKAIIKT 681
             + K WFLSHAQYLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWEFF AT+IGKAIIKT
Sbjct: 248  NKTKLWFLSHAQYLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWEFFFATIIGKAIIKT 307

Query: 680  HIQTLFIISVCNNQLLDWVENELIWMLSFVPGFDSILPNIVAKLHSMKDKYLATK-SRVP 504
            HIQT+FII VCNNQLL W+ENEL+W+LS +PGF SI+P+++ KLH M+DKYLATK + +P
Sbjct: 308  HIQTVFIICVCNNQLLYWIENELLWVLSSLPGFSSIIPSLITKLHLMQDKYLATKPNNIP 367

Query: 503  LNVKAKKWDFSLAFIWNTVVWFMLMNFFVKIVNATAQRYAKKNQEKELAALENQS 339
             NV+ KKWDFSLA +WNTVVW ML+NFFVKIVN+TAQRY KK Q+KE+ A++N+S
Sbjct: 368  SNVEVKKWDFSLALVWNTVVWIMLLNFFVKIVNSTAQRYLKKLQDKEIDAVKNKS 422


>ref|XP_006489364.1| PREDICTED: LOW QUALITY PROTEIN: vacuole membrane protein 1-like
            [Citrus sinensis]
          Length = 425

 Score =  636 bits (1641), Expect = e-179
 Identities = 308/415 (74%), Positives = 357/415 (86%), Gaps = 1/415 (0%)
 Frame = -2

Query: 1580 ENSPSGSEDRDMSISGLHWKHQQELENLTLTSQPIKTLKLCTLAIIQYLRQSVAYLLSHG 1401
            + + S S+D  MSIS L  KHQQELENLTLT+QP KT+KL  L +IQYL++SV YLL+ G
Sbjct: 5    KGATSSSQDMAMSISDLREKHQQELENLTLTTQPFKTIKLFILGVIQYLKRSVLYLLAKG 64

Query: 1400 GWLMLLSTMIVIAGVF-VTTNGPHEKHVEEVLRYVRFGLWWMALGVASSIGLGSGLHTFV 1224
            GWLML  T++   G+  VT +GPHEKH+EE+  Y RFGLWW+ALGVASSIGLGSGLHTFV
Sbjct: 65   GWLMLSCTVVAALGILLVTIDGPHEKHLEELSEYFRFGLWWVALGVASSIGLGSGLHTFV 124

Query: 1223 LYLGPHIALFTIKAMQCGRVDIKSAPYDTIQLKRPPSWLGKDCAEFGPPLFSSSHGTQVP 1044
            LYLGPHIALFTIKAMQCGRVD+KSAPYDTIQLKR PSWL KDC EFGPPLFSSS G++VP
Sbjct: 125  LYLGPHIALFTIKAMQCGRVDLKSAPYDTIQLKRGPSWLDKDCDEFGPPLFSSSEGSRVP 184

Query: 1043 LSAILPQVQLEAILWGLGTALGELPPYFISRAASLSGGKLDAMEELDASSTEDNGFMAAR 864
            LS+ILPQVQLEAILWG+GTALGELPPYFISRAAS+SG +LDAM+E D SSTE +G +AA 
Sbjct: 185  LSSILPQVQLEAILWGVGTALGELPPYFISRAASISGSRLDAMDEFDDSSTESDGVVAAX 244

Query: 863  LTQIKRWFLSHAQYLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKAIIK 684
            L +IKRWF +H+Q+LNF TILVLASVPNPLFDLAGIMCGQFG+PFW+FFLAT+IGKAIIK
Sbjct: 245  LKKIKRWFFTHSQHLNFLTILVLASVPNPLFDLAGIMCGQFGVPFWKFFLATLIGKAIIK 304

Query: 683  THIQTLFIISVCNNQLLDWVENELIWMLSFVPGFDSILPNIVAKLHSMKDKYLATKSRVP 504
            THIQT+FIISVCNNQLLDW+ENELIW+LS VPGF  +LP++VAKLH+M+ KYL  + +  
Sbjct: 305  THIQTVFIISVCNNQLLDWIENELIWVLSLVPGFAPVLPSLVAKLHTMRAKYLKPQ-QPS 363

Query: 503  LNVKAKKWDFSLAFIWNTVVWFMLMNFFVKIVNATAQRYAKKNQEKELAALENQS 339
             ++K KKWDFS A +WNT+VW MLMNFFVKIV  TAQRY KK Q+KE+AAL N S
Sbjct: 364  SHIKVKKWDFSFASVWNTIVWLMLMNFFVKIVTDTAQRYLKKQQDKEIAALTNNS 418


>ref|XP_007034717.1| SNARE associated Golgi protein family isoform 1 [Theobroma cacao]
            gi|508713746|gb|EOY05643.1| SNARE associated Golgi
            protein family isoform 1 [Theobroma cacao]
          Length = 418

 Score =  636 bits (1641), Expect = e-179
 Identities = 313/409 (76%), Positives = 349/409 (85%), Gaps = 1/409 (0%)
 Frame = -2

Query: 1580 ENSPSGSEDRDMSISGLHWKHQQELENLTLTSQPIKTLKLCTLAIIQYLRQSVAYLLSHG 1401
            + + S S+  D SI  L  +HQQELE+LTLT+QP KTL+L  LAI QY ++SV YLL+ G
Sbjct: 5    KGATSSSQQMDTSIEDLRERHQQELEHLTLTTQPFKTLRLFILAIFQYFKRSVLYLLAKG 64

Query: 1400 GWLMLLSTMIVIAGVF-VTTNGPHEKHVEEVLRYVRFGLWWMALGVASSIGLGSGLHTFV 1224
            GWLMLLST+I   G+  VT  GPHEKHVEEV RYVRFGLWW ALGVASSIGLGSGLHTFV
Sbjct: 65   GWLMLLSTLIAAFGILLVTIEGPHEKHVEEVSRYVRFGLWWTALGVASSIGLGSGLHTFV 124

Query: 1223 LYLGPHIALFTIKAMQCGRVDIKSAPYDTIQLKRPPSWLGKDCAEFGPPLFSSSHGTQVP 1044
            LYLGPHIALFTIKAMQCGRVD+KSAPYDTIQLKR PSWL K C EFGPPL+    G+ VP
Sbjct: 125  LYLGPHIALFTIKAMQCGRVDLKSAPYDTIQLKRGPSWLDKSCGEFGPPLY----GSWVP 180

Query: 1043 LSAILPQVQLEAILWGLGTALGELPPYFISRAASLSGGKLDAMEELDASSTEDNGFMAAR 864
            LS+IL QVQ+EAILWG+GTALGELPPYFISRAA +SG K+DAMEELDASS EDNGF+A  
Sbjct: 181  LSSILRQVQMEAILWGIGTALGELPPYFISRAARVSGSKIDAMEELDASSIEDNGFIATH 240

Query: 863  LTQIKRWFLSHAQYLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKAIIK 684
            L QIKRW LSH+Q+LNFFTILVLASVPNPLFDLAGIMCGQFGIPFW+FFLAT+ GKAIIK
Sbjct: 241  LKQIKRWLLSHSQHLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWKFFLATLFGKAIIK 300

Query: 683  THIQTLFIISVCNNQLLDWVENELIWMLSFVPGFDSILPNIVAKLHSMKDKYLATKSRVP 504
            THIQT+FIISVCNNQLLDW+ENELIW+LS +PGFDS LP + AKLH++K+KYL     VP
Sbjct: 301  THIQTVFIISVCNNQLLDWIENELIWVLSLIPGFDSFLPTLTAKLHAVKEKYLTAPRPVP 360

Query: 503  LNVKAKKWDFSLAFIWNTVVWFMLMNFFVKIVNATAQRYAKKNQEKELA 357
             N K +KWDFS AFIWNTVVW MLMNFFVKIVNATAQRY KK Q+K+LA
Sbjct: 361  SNSK-EKWDFSFAFIWNTVVWLMLMNFFVKIVNATAQRYLKKEQDKQLA 408


>ref|XP_011021552.1| PREDICTED: vacuole membrane protein KMS1 [Populus euphratica]
          Length = 429

 Score =  632 bits (1631), Expect = e-178
 Identities = 304/415 (73%), Positives = 354/415 (85%), Gaps = 1/415 (0%)
 Frame = -2

Query: 1580 ENSPSGSEDRDMSISGLHWKHQQELENLTLTSQPIKTLKLCTLAIIQYLRQSVAYLLSHG 1401
            E + S S++ DMSI+ LH +HQQELENLT+T QP KTLK   LA+IQY ++SV YLL+ G
Sbjct: 8    EATASSSQNMDMSIAALHERHQQELENLTMTGQPFKTLKFFVLAMIQYCKRSVFYLLAKG 67

Query: 1400 GWLMLLSTMIVIAG-VFVTTNGPHEKHVEEVLRYVRFGLWWMALGVASSIGLGSGLHTFV 1224
            GWLMLLST++  AG V VT +GPHEKHVEE+  Y+RFGLWW+ALGVASSIGLGSGLHTFV
Sbjct: 68   GWLMLLSTVVAAAGIVLVTIDGPHEKHVEELSNYLRFGLWWIALGVASSIGLGSGLHTFV 127

Query: 1223 LYLGPHIALFTIKAMQCGRVDIKSAPYDTIQLKRPPSWLGKDCAEFGPPLFSSSHGTQVP 1044
            LYLGPHIALFTIKAMQCGRVD+KSAPYDTIQLKR PSWLGKDC EFG P+F  SHG QVP
Sbjct: 128  LYLGPHIALFTIKAMQCGRVDLKSAPYDTIQLKRVPSWLGKDCKEFGDPMFPFSHGLQVP 187

Query: 1043 LSAILPQVQLEAILWGLGTALGELPPYFISRAASLSGGKLDAMEELDASSTEDNGFMAAR 864
            +S+IL QVQ+EAILWG+GTALGELPPYFISRAA +SG KLDAMEELDAS  E++G +A R
Sbjct: 188  ISSILFQVQIEAILWGVGTALGELPPYFISRAARISGSKLDAMEELDASLDENSGIIATR 247

Query: 863  LTQIKRWFLSHAQYLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKAIIK 684
            LT IK W L+H+Q+LNFFTILVLASVPNPLFDLAGIMCGQFG+PFW+FF AT+IGKAI+K
Sbjct: 248  LTAIKHWLLTHSQHLNFFTILVLASVPNPLFDLAGIMCGQFGVPFWKFFAATLIGKAIVK 307

Query: 683  THIQTLFIISVCNNQLLDWVENELIWMLSFVPGFDSILPNIVAKLHSMKDKYLATKSRVP 504
            THIQT+FIISVCNNQLL+W+ENELIW+LS +PGF   LP++ AKLH+MK KY+A  S   
Sbjct: 308  THIQTIFIISVCNNQLLNWIENELIWVLSLIPGFAPALPHLTAKLHAMKAKYMAPASPAS 367

Query: 503  LNVKAKKWDFSLAFIWNTVVWFMLMNFFVKIVNATAQRYAKKNQEKELAALENQS 339
             ++K K WDFS A +WNTVVW ML+NFF KIVN+TAQ Y KK QEKE+AAL + +
Sbjct: 368  SDMKVKNWDFSFAGMWNTVVWLMLVNFFFKIVNSTAQSYLKKQQEKEIAALTDSA 422


>ref|XP_002314707.2| hypothetical protein POPTR_0010s09980g [Populus trichocarpa]
            gi|550329478|gb|EEF00878.2| hypothetical protein
            POPTR_0010s09980g [Populus trichocarpa]
          Length = 429

 Score =  631 bits (1627), Expect = e-178
 Identities = 301/415 (72%), Positives = 352/415 (84%), Gaps = 1/415 (0%)
 Frame = -2

Query: 1580 ENSPSGSEDRDMSISGLHWKHQQELENLTLTSQPIKTLKLCTLAIIQYLRQSVAYLLSHG 1401
            E   S S++ DMSI+GLH +HQQELENLT+T QP KTLK   LA++QY ++SV YLL+ G
Sbjct: 8    EAKASSSQNMDMSIAGLHERHQQELENLTMTGQPFKTLKFFVLAMVQYCKRSVFYLLAKG 67

Query: 1400 GWLMLLSTMIVIAG-VFVTTNGPHEKHVEEVLRYVRFGLWWMALGVASSIGLGSGLHTFV 1224
            GWLMLLST++   G V VT +GPHEKHVEE+  Y+RFGLWW+ALGVASSIGLGSGLHTFV
Sbjct: 68   GWLMLLSTVVAAVGIVLVTIDGPHEKHVEELSNYLRFGLWWIALGVASSIGLGSGLHTFV 127

Query: 1223 LYLGPHIALFTIKAMQCGRVDIKSAPYDTIQLKRPPSWLGKDCAEFGPPLFSSSHGTQVP 1044
            LYLGPHIALFTIKAMQCGRVD+KSAPYDTIQLKR PSWLGKDC EFG P+F  SHG +VP
Sbjct: 128  LYLGPHIALFTIKAMQCGRVDLKSAPYDTIQLKRVPSWLGKDCKEFGDPVFPFSHGLRVP 187

Query: 1043 LSAILPQVQLEAILWGLGTALGELPPYFISRAASLSGGKLDAMEELDASSTEDNGFMAAR 864
            +S+IL QVQ+EAILWG+GTALGELPPYFISRAA +SG KLDAMEELDAS  E++G +A R
Sbjct: 188  ISSILFQVQIEAILWGVGTALGELPPYFISRAARISGSKLDAMEELDASLDENSGIIATR 247

Query: 863  LTQIKRWFLSHAQYLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKAIIK 684
            LT IK W L+H+Q+LNFFTILVLASVPNPLFDLAGIMCGQFG+PFW+FF AT+IGKAI+K
Sbjct: 248  LTAIKHWLLTHSQHLNFFTILVLASVPNPLFDLAGIMCGQFGVPFWKFFAATLIGKAIVK 307

Query: 683  THIQTLFIISVCNNQLLDWVENELIWMLSFVPGFDSILPNIVAKLHSMKDKYLATKSRVP 504
            THIQT+FIISVCNNQLL+W+ENELIW+LS +PGF   LP++ AKLH+MK+KY+A      
Sbjct: 308  THIQTIFIISVCNNQLLNWIENELIWVLSLIPGFAPALPHLTAKLHAMKEKYMAPAPPAS 367

Query: 503  LNVKAKKWDFSLAFIWNTVVWFMLMNFFVKIVNATAQRYAKKNQEKELAALENQS 339
             ++K K WDFS A +WNTVVW ML+NFF KIVN+TAQ Y KK QEKE+ AL N +
Sbjct: 368  SDMKVKNWDFSFAGMWNTVVWLMLLNFFFKIVNSTAQSYLKKQQEKEIGALTNSA 422


>gb|KHG15586.1| Vacuole membrane 1 [Gossypium arboreum]
          Length = 426

 Score =  629 bits (1623), Expect = e-177
 Identities = 303/414 (73%), Positives = 356/414 (85%), Gaps = 4/414 (0%)
 Frame = -2

Query: 1580 ENSPSGSEDRDMSISGLHWKHQQELENLTLTSQPIKTLKLCTLAIIQYLRQSVAYLLSHG 1401
            + + S ++  + S+  L  +++ ELENLTLT+QP+KT +   LAI+QY+++S +YLL+ G
Sbjct: 5    KGATSSAQQMESSVQDLRERYRLELENLTLTTQPVKTSRFFLLAILQYIKRSASYLLAKG 64

Query: 1400 GWLMLLSTMIVIAGVF-VTTNGPHEKH---VEEVLRYVRFGLWWMALGVASSIGLGSGLH 1233
            GWLML ST+I + G+  VT  GPHEKH   VEEV +YVRFGLWW+ALGVASSIGLGSGLH
Sbjct: 65   GWLMLSSTLIAVLGILLVTIEGPHEKHTSHVEEVSQYVRFGLWWIALGVASSIGLGSGLH 124

Query: 1232 TFVLYLGPHIALFTIKAMQCGRVDIKSAPYDTIQLKRPPSWLGKDCAEFGPPLFSSSHGT 1053
            TFVLYLGPHIALFTIKAMQCGR+D+KSAPYDTIQLKR PSWL K C EFGPPLFSSSHG+
Sbjct: 125  TFVLYLGPHIALFTIKAMQCGRIDLKSAPYDTIQLKRVPSWLDKPCREFGPPLFSSSHGS 184

Query: 1052 QVPLSAILPQVQLEAILWGLGTALGELPPYFISRAASLSGGKLDAMEELDASSTEDNGFM 873
            +VP+S+ILPQVQ+EAILWGLGTALGELPPYFISRAAS+SG K++ MEELD SS+EDNGF+
Sbjct: 185  RVPISSILPQVQIEAILWGLGTALGELPPYFISRAASVSGRKIEPMEELDHSSSEDNGFI 244

Query: 872  AARLTQIKRWFLSHAQYLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKA 693
            A  L QI+ W LSH+Q++NFFTIL+LASVPNPLFDLAGIMCGQFGIPFW+FFLAT+IGKA
Sbjct: 245  ATHLKQIEHWLLSHSQHMNFFTILLLASVPNPLFDLAGIMCGQFGIPFWKFFLATLIGKA 304

Query: 692  IIKTHIQTLFIISVCNNQLLDWVENELIWMLSFVPGFDSILPNIVAKLHSMKDKYLATKS 513
            IIKTHIQT+FIISVCNNQLLDW+ENELIW+LSF+P FDS LP + AKLHS+K+KYLA   
Sbjct: 305  IIKTHIQTVFIISVCNNQLLDWIENELIWILSFIPRFDSYLPTLTAKLHSVKEKYLAAPP 364

Query: 512  RVPLNVKAKKWDFSLAFIWNTVVWFMLMNFFVKIVNATAQRYAKKNQEKELAAL 351
             VP N+K  KWDFS A IWNT+VW MLMNFFVKIVNATAQRY KK Q+K LA +
Sbjct: 365  PVPSNIK-DKWDFSFASIWNTIVWLMLMNFFVKIVNATAQRYLKKQQDKSLAEI 417


>ref|XP_012069605.1| PREDICTED: vacuole membrane protein KMS1 isoform X2 [Jatropha curcas]
          Length = 426

 Score =  627 bits (1618), Expect = e-177
 Identities = 300/413 (72%), Positives = 353/413 (85%), Gaps = 1/413 (0%)
 Frame = -2

Query: 1580 ENSPSGSEDRDMSISGLHWKHQQELENLTLTSQPIKTLKLCTLAIIQYLRQSVAYLLSHG 1401
            + + S S++ D+SIS LH +HQQELENLTLT+QP KTLK   LA+IQY +QS+ YLL+ G
Sbjct: 7    KGATSSSQNMDISISDLHERHQQELENLTLTTQPFKTLKFFILAMIQYCKQSILYLLAKG 66

Query: 1400 GWLMLLSTMIVIAGVF-VTTNGPHEKHVEEVLRYVRFGLWWMALGVASSIGLGSGLHTFV 1224
            GWLML+ST++   G+  VT +GPHEKH+EE   Y+RFGLWW+ALGVASSIGLGSGLHTFV
Sbjct: 67   GWLMLISTVVAALGILLVTIDGPHEKHIEEFSHYIRFGLWWIALGVASSIGLGSGLHTFV 126

Query: 1223 LYLGPHIALFTIKAMQCGRVDIKSAPYDTIQLKRPPSWLGKDCAEFGPPLFSSSHGTQVP 1044
            LYLGPHIALFTIKAMQCGRVD+KSA YDTIQLKR PSWL KDC+EFG P+F SSHG +VP
Sbjct: 127  LYLGPHIALFTIKAMQCGRVDLKSAVYDTIQLKRGPSWLDKDCSEFGDPVFPSSHGLRVP 186

Query: 1043 LSAILPQVQLEAILWGLGTALGELPPYFISRAASLSGGKLDAMEELDASSTEDNGFMAAR 864
            LS+ILPQVQ+EA+LWGLGTALGELPPYFISRAAS+SG KL+AMEE +ASS+EDNG +A  
Sbjct: 187  LSSILPQVQIEAVLWGLGTALGELPPYFISRAASISGNKLEAMEEFNASSSEDNGIIATH 246

Query: 863  LTQIKRWFLSHAQYLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKAIIK 684
            L  IKRW LSHAQ+LNF TIL+LASVPNPLFDLAGIMCGQFGI FW+FFLAT+IGKA++K
Sbjct: 247  LNTIKRWLLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGISFWKFFLATLIGKALVK 306

Query: 683  THIQTLFIISVCNNQLLDWVENELIWMLSFVPGFDSILPNIVAKLHSMKDKYLATKSRVP 504
            THIQT+FIISVCNNQLL+W+ENELI +LS VPG  SILP++VAKLH++K+KY+A    +P
Sbjct: 307  THIQTVFIISVCNNQLLNWIENELIMILSLVPGLASILPDLVAKLHAIKEKYMAPVPPLP 366

Query: 503  LNVKAKKWDFSLAFIWNTVVWFMLMNFFVKIVNATAQRYAKKNQEKELAALEN 345
             N K KKWDFS A +WNT+VW ML+NFF KIV ATAQ Y KK QE ++A L N
Sbjct: 367  SNNKMKKWDFSFAALWNTIVWLMLLNFFFKIVTATAQSYLKKQQENQIAELTN 419


>gb|KDP40157.1| hypothetical protein JCGZ_02155 [Jatropha curcas]
          Length = 424

 Score =  627 bits (1618), Expect = e-177
 Identities = 300/413 (72%), Positives = 353/413 (85%), Gaps = 1/413 (0%)
 Frame = -2

Query: 1580 ENSPSGSEDRDMSISGLHWKHQQELENLTLTSQPIKTLKLCTLAIIQYLRQSVAYLLSHG 1401
            + + S S++ D+SIS LH +HQQELENLTLT+QP KTLK   LA+IQY +QS+ YLL+ G
Sbjct: 5    KGATSSSQNMDISISDLHERHQQELENLTLTTQPFKTLKFFILAMIQYCKQSILYLLAKG 64

Query: 1400 GWLMLLSTMIVIAGVF-VTTNGPHEKHVEEVLRYVRFGLWWMALGVASSIGLGSGLHTFV 1224
            GWLML+ST++   G+  VT +GPHEKH+EE   Y+RFGLWW+ALGVASSIGLGSGLHTFV
Sbjct: 65   GWLMLISTVVAALGILLVTIDGPHEKHIEEFSHYIRFGLWWIALGVASSIGLGSGLHTFV 124

Query: 1223 LYLGPHIALFTIKAMQCGRVDIKSAPYDTIQLKRPPSWLGKDCAEFGPPLFSSSHGTQVP 1044
            LYLGPHIALFTIKAMQCGRVD+KSA YDTIQLKR PSWL KDC+EFG P+F SSHG +VP
Sbjct: 125  LYLGPHIALFTIKAMQCGRVDLKSAVYDTIQLKRGPSWLDKDCSEFGDPVFPSSHGLRVP 184

Query: 1043 LSAILPQVQLEAILWGLGTALGELPPYFISRAASLSGGKLDAMEELDASSTEDNGFMAAR 864
            LS+ILPQVQ+EA+LWGLGTALGELPPYFISRAAS+SG KL+AMEE +ASS+EDNG +A  
Sbjct: 185  LSSILPQVQIEAVLWGLGTALGELPPYFISRAASISGNKLEAMEEFNASSSEDNGIIATH 244

Query: 863  LTQIKRWFLSHAQYLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKAIIK 684
            L  IKRW LSHAQ+LNF TIL+LASVPNPLFDLAGIMCGQFGI FW+FFLAT+IGKA++K
Sbjct: 245  LNTIKRWLLSHAQHLNFLTILLLASVPNPLFDLAGIMCGQFGISFWKFFLATLIGKALVK 304

Query: 683  THIQTLFIISVCNNQLLDWVENELIWMLSFVPGFDSILPNIVAKLHSMKDKYLATKSRVP 504
            THIQT+FIISVCNNQLL+W+ENELI +LS VPG  SILP++VAKLH++K+KY+A    +P
Sbjct: 305  THIQTVFIISVCNNQLLNWIENELIMILSLVPGLASILPDLVAKLHAIKEKYMAPVPPLP 364

Query: 503  LNVKAKKWDFSLAFIWNTVVWFMLMNFFVKIVNATAQRYAKKNQEKELAALEN 345
             N K KKWDFS A +WNT+VW ML+NFF KIV ATAQ Y KK QE ++A L N
Sbjct: 365  SNNKMKKWDFSFAALWNTIVWLMLLNFFFKIVTATAQSYLKKQQENQIAELTN 417


>ref|NP_567450.1| ER associated membrane protein KMS1 [Arabidopsis thaliana]
            gi|75110971|sp|Q5XF36.1|KMS1_ARATH RecName: Full=Vacuole
            membrane protein KMS1; AltName: Full=Protein KILLING ME
            SLOWLY 1 gi|53749170|gb|AAU90070.1| At4g14950
            [Arabidopsis thaliana] gi|332658128|gb|AEE83528.1| ER
            associated membrane protein KMS1 [Arabidopsis thaliana]
          Length = 416

 Score =  627 bits (1616), Expect = e-176
 Identities = 295/405 (72%), Positives = 352/405 (86%), Gaps = 1/405 (0%)
 Frame = -2

Query: 1562 SEDRDMSISGLHWKHQQELENLTLTSQPIKTLKLCTLAIIQYLRQSVAYLLSHGGWLMLL 1383
            S   D++IS L  KH++E+ENLTLT+QP+ TLKL   A IQY+++S++YLL+HGGW +L+
Sbjct: 8    SSSSDVAISALREKHEKEVENLTLTTQPLNTLKLFVEATIQYIKRSISYLLAHGGWFILI 67

Query: 1382 STMIVIAG-VFVTTNGPHEKHVEEVLRYVRFGLWWMALGVASSIGLGSGLHTFVLYLGPH 1206
            +T++V++G + VT +GPH KHVEEVL YVR+GLWW+ALGVASSIGLGSGLHTFVLYLGPH
Sbjct: 68   TTLLVVSGGLLVTVDGPHGKHVEEVLEYVRYGLWWIALGVASSIGLGSGLHTFVLYLGPH 127

Query: 1205 IALFTIKAMQCGRVDIKSAPYDTIQLKRPPSWLGKDCAEFGPPLFSSSHGTQVPLSAILP 1026
            IALFT+KA  CGRVD+KSAPYDTIQLKR PSWL K C+EFGPPL  S+ G++VPL++ILP
Sbjct: 128  IALFTLKATLCGRVDLKSAPYDTIQLKRVPSWLDKSCSEFGPPLMISAAGSRVPLTSILP 187

Query: 1025 QVQLEAILWGLGTALGELPPYFISRAASLSGGKLDAMEELDASSTEDNGFMAARLTQIKR 846
            QVQLEAILWG+GTALGELPPYFISRAAS+SG  +D MEELD SSTED+GFMA  L ++KR
Sbjct: 188  QVQLEAILWGIGTALGELPPYFISRAASISGSTVDGMEELDGSSTEDSGFMATHLNRVKR 247

Query: 845  WFLSHAQYLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKAIIKTHIQTL 666
            W L+H+Q+LNFFT+LVLASVPNPLFDLAGIMCGQFGIPFWEFFLAT+IGKAIIKTHIQT+
Sbjct: 248  WLLTHSQHLNFFTVLVLASVPNPLFDLAGIMCGQFGIPFWEFFLATLIGKAIIKTHIQTI 307

Query: 665  FIISVCNNQLLDWVENELIWMLSFVPGFDSILPNIVAKLHSMKDKYLATKSRVPLNVKAK 486
            FII VCNNQLLDW+ENELIW+LS VPG  S+LP + AKLH+MK+KY+   S VP ++K K
Sbjct: 308  FIICVCNNQLLDWMENELIWILSHVPGLASMLPGLTAKLHAMKEKYIDAPSPVPSHIKVK 367

Query: 485  KWDFSLAFIWNTVVWFMLMNFFVKIVNATAQRYAKKNQEKELAAL 351
            KWDFS A IWN +VW ML+NFFVKIV ATAQR+ KK QEKE+A L
Sbjct: 368  KWDFSFASIWNGIVWLMLLNFFVKIVTATAQRHLKKKQEKEMATL 412


>ref|XP_002281783.1| PREDICTED: vacuole membrane protein KMS1 [Vitis vinifera]
            gi|297737027|emb|CBI26228.3| unnamed protein product
            [Vitis vinifera]
          Length = 439

 Score =  625 bits (1613), Expect = e-176
 Identities = 300/424 (70%), Positives = 359/424 (84%), Gaps = 1/424 (0%)
 Frame = -2

Query: 1577 NSPSGSEDRDMSISGLHWKHQQELENLTLTSQPIKTLKLCTLAIIQYLRQSVAYLLSHGG 1398
            ++ S S   +M+ISGL  + QQE+ENLTL ++P K LK   L ++Q+++QSVAYLL+ GG
Sbjct: 12   SASSSSGSLEMAISGLRERQQQEMENLTLKTEPFKVLKFFILVVVQHVKQSVAYLLAKGG 71

Query: 1397 WLMLLSTMIVIAG-VFVTTNGPHEKHVEEVLRYVRFGLWWMALGVASSIGLGSGLHTFVL 1221
            W+MLLS + +  G V +  +GPHEKHV+E+ +Y  FGLWW+ALG+ASSIGLGSGLHTFVL
Sbjct: 72   WVMLLSVVAMALGFVLMAIDGPHEKHVQELHQYFLFGLWWIALGIASSIGLGSGLHTFVL 131

Query: 1220 YLGPHIALFTIKAMQCGRVDIKSAPYDTIQLKRPPSWLGKDCAEFGPPLFSSSHGTQVPL 1041
            YLGPHIALFTIKAMQCGRVDIKSAPYDTI+LK  PSWL KDC+EFGPP+FSS H  +VPL
Sbjct: 132  YLGPHIALFTIKAMQCGRVDIKSAPYDTIRLKSGPSWLDKDCSEFGPPVFSSLHALRVPL 191

Query: 1040 SAILPQVQLEAILWGLGTALGELPPYFISRAASLSGGKLDAMEELDASSTEDNGFMAARL 861
            S+ILPQVQ+EA+LWG+GTALGELPPYFIS+AASLSG K DA+EELDASS ED+G ++  L
Sbjct: 192  SSILPQVQVEAVLWGIGTALGELPPYFISKAASLSGSKSDAIEELDASSPEDDGVVSTHL 251

Query: 860  TQIKRWFLSHAQYLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKAIIKT 681
             QIKRWFLSH+Q+LNFFTILVLASVPNPLFDLAGIMCGQFGIPFW+FFLAT IGKAIIKT
Sbjct: 252  KQIKRWFLSHSQHLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWKFFLATFIGKAIIKT 311

Query: 680  HIQTLFIISVCNNQLLDWVENELIWMLSFVPGFDSILPNIVAKLHSMKDKYLATKSRVPL 501
            HIQT+FIISVCNNQLLDW+ENELIW+LS +PGF S+LPN+++KL++M+DKYLA    VP 
Sbjct: 312  HIQTVFIISVCNNQLLDWIENELIWVLSLIPGFASVLPNLISKLNAMRDKYLAPSPPVPS 371

Query: 500  NVKAKKWDFSLAFIWNTVVWFMLMNFFVKIVNATAQRYAKKNQEKELAALENQS*EHSRD 321
            N+K KKWD S A IWNTVVW ML+NFF KIV ATAQ + KK QEKEL AL+++  E +  
Sbjct: 372  NMKVKKWDLSFASIWNTVVWLMLINFFFKIVTATAQNFLKKQQEKELLALKDELSESTVS 431

Query: 320  SDHS 309
            +D S
Sbjct: 432  NDQS 435


>gb|AAL24087.1| unknown protein [Arabidopsis thaliana]
          Length = 416

 Score =  625 bits (1612), Expect = e-176
 Identities = 295/405 (72%), Positives = 352/405 (86%), Gaps = 1/405 (0%)
 Frame = -2

Query: 1562 SEDRDMSISGLHWKHQQELENLTLTSQPIKTLKLCTLAIIQYLRQSVAYLLSHGGWLMLL 1383
            S   D++IS L  KH++E+ENLTLT+QP+ TLKL   A IQY+++S++YLL+HGGW +L+
Sbjct: 8    SSSSDVAISALREKHEKEVENLTLTTQPLNTLKLFVEATIQYIKRSISYLLAHGGWFILI 67

Query: 1382 STMIVIAG-VFVTTNGPHEKHVEEVLRYVRFGLWWMALGVASSIGLGSGLHTFVLYLGPH 1206
            +T++V++G + VT +GPH KHVEEVL YVR+GLWW+ALGVASSIGLGSGLHTFVLYLGPH
Sbjct: 68   TTLLVVSGGLLVTVDGPHGKHVEEVLGYVRYGLWWIALGVASSIGLGSGLHTFVLYLGPH 127

Query: 1205 IALFTIKAMQCGRVDIKSAPYDTIQLKRPPSWLGKDCAEFGPPLFSSSHGTQVPLSAILP 1026
            IALFT+KA  CGRVD+KSAPYDTIQLKR PSWL K C+EFGPPL  S+ G++VPL++ILP
Sbjct: 128  IALFTLKATLCGRVDLKSAPYDTIQLKRVPSWLDKSCSEFGPPLMISAAGSRVPLTSILP 187

Query: 1025 QVQLEAILWGLGTALGELPPYFISRAASLSGGKLDAMEELDASSTEDNGFMAARLTQIKR 846
            QVQLEAILWG+GTALGELPPYFISRAAS+SG  +D MEELD SSTED+GFMA  L ++KR
Sbjct: 188  QVQLEAILWGIGTALGELPPYFISRAASISGSTVDGMEELDGSSTEDSGFMATHLNRVKR 247

Query: 845  WFLSHAQYLNFFTILVLASVPNPLFDLAGIMCGQFGIPFWEFFLATMIGKAIIKTHIQTL 666
            W L+H+Q+LNFFT+LVLASVPNPLFDLAGIMCGQFGIPFWEFFLAT+IGKAIIKTHIQT+
Sbjct: 248  WLLTHSQHLNFFTVLVLASVPNPLFDLAGIMCGQFGIPFWEFFLATLIGKAIIKTHIQTI 307

Query: 665  FIISVCNNQLLDWVENELIWMLSFVPGFDSILPNIVAKLHSMKDKYLATKSRVPLNVKAK 486
            FII VCNNQLLDW+ENELIW+LS VPG  S+LP + AKLH+MK+KY+   S VP ++K K
Sbjct: 308  FIICVCNNQLLDWMENELIWILSHVPGLASMLPGLTAKLHAMKEKYIDAPSPVPSHIKVK 367

Query: 485  KWDFSLAFIWNTVVWFMLMNFFVKIVNATAQRYAKKNQEKELAAL 351
            KWDFS A IWN +VW ML+NFFVKIV ATAQR+ KK QEKE+A L
Sbjct: 368  KWDFSFASIWNGMVWLMLLNFFVKIVTATAQRHLKKKQEKEMATL 412