BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ephedra29_contig00027501
         (335 letters)

Database: ./nr 
           115,041,592 sequences; 42,171,959,267 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

JAU72630.1 Kinesin-like protein KIN12A, partial [Noccaea caerule...   179   1e-55
ERN10032.1 hypothetical protein AMTR_s00013p00246030 [Amborella ...   189   2e-53
XP_006848451.2 PREDICTED: kinesin-like protein KIN12B [Amborella...   189   2e-53
KNA24096.1 hypothetical protein SOVF_019010 [Spinacia oleracea]       187   6e-53
XP_010650335.1 PREDICTED: kinesin-like protein KIN-12B [Vitis vi...   186   1e-52
CBI17294.3 unnamed protein product, partial [Vitis vinifera]          186   1e-52
JAU77566.1 Kinesin-like protein KIN12B, partial [Noccaea caerule...   179   2e-52
OAE28312.1 hypothetical protein AXG93_2490s1000 [Marchantia poly...   185   4e-52
XP_010246668.1 PREDICTED: kinesin-like protein KIN-12B [Nelumbo ...   183   1e-51
OAY81494.1 Kinesin-like protein KIN12B [Ananas comosus]               178   1e-51
XP_010907239.1 PREDICTED: kinesin-like protein KIN-12B [Elaeis g...   183   2e-51
XP_009415942.1 PREDICTED: kinesin-like protein KIN-12A [Musa acu...   182   2e-51
XP_020112122.1 kinesin-like protein KIN-12A [Ananas comosus]          182   3e-51
OAY69167.1 Kinesin-like protein KIN12B [Ananas comosus]               182   3e-51
OAY71388.1 Kinesin-like protein KIN12B, partial [Ananas comosus]      181   5e-51
JAU41170.1 Kinesin-like protein KIN12B, partial [Noccaea caerule...   179   6e-51
XP_010326953.1 PREDICTED: kinesin-like protein KIN-12B [Solanum ...   181   6e-51
XP_015086149.1 PREDICTED: kinesin-like protein KIN12B [Solanum p...   181   6e-51
XP_006358791.1 PREDICTED: kinesin-like protein KIN12B [Solanum t...   181   8e-51
XP_016566233.1 PREDICTED: kinesin-like protein KIN12B [Capsicum ...   181   8e-51

>JAU72630.1 Kinesin-like protein KIN12A, partial [Noccaea caerulescens]
          Length = 170

 Score =  179 bits (454), Expect = 1e-55
 Identities = 92/111 (82%), Positives = 101/111 (90%)
 Frame = -1

Query: 335 AGNINRSLSQLGNLIKILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMICSISPA 156
           AGNINRSLSQLGNLI ILAE+SQTGK RHIPYRDSRLTFLLQESLGGNAKLAM+C+ISP+
Sbjct: 17  AGNINRSLSQLGNLINILAEISQTGKPRHIPYRDSRLTFLLQESLGGNAKLAMVCAISPS 76

Query: 155 ASCRSETLSTLRFAQRAKAIKNKAVVNEETTESDVNFLRGQIRQLKNELMR 3
            SC+SET STLRFAQRAKAI+NKAVVN E  + DVNFLRG IR+LK+EL R
Sbjct: 77  QSCKSETFSTLRFAQRAKAIQNKAVVN-EVMQDDVNFLRGVIRELKDELQR 126


>ERN10032.1 hypothetical protein AMTR_s00013p00246030 [Amborella trichopoda]
          Length = 1309

 Score =  189 bits (479), Expect = 2e-53
 Identities = 100/111 (90%), Positives = 105/111 (94%)
 Frame = -1

Query: 335 AGNINRSLSQLGNLIKILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMICSISPA 156
           AGNINRSLSQLGNLI ILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMIC+ISPA
Sbjct: 354 AGNINRSLSQLGNLINILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMICAISPA 413

Query: 155 ASCRSETLSTLRFAQRAKAIKNKAVVNEETTESDVNFLRGQIRQLKNELMR 3
            SC+SETLSTLRFAQRAKAI+NKAVVN ETTE DVN LR QIRQLK+EL+R
Sbjct: 414 QSCKSETLSTLRFAQRAKAIQNKAVVN-ETTEDDVNVLREQIRQLKDELIR 463


>XP_006848451.2 PREDICTED: kinesin-like protein KIN12B [Amborella trichopoda]
          Length = 1269

 Score =  189 bits (479), Expect = 2e-53
 Identities = 100/111 (90%), Positives = 105/111 (94%)
 Frame = -1

Query: 335 AGNINRSLSQLGNLIKILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMICSISPA 156
           AGNINRSLSQLGNLI ILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMIC+ISPA
Sbjct: 314 AGNINRSLSQLGNLINILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMICAISPA 373

Query: 155 ASCRSETLSTLRFAQRAKAIKNKAVVNEETTESDVNFLRGQIRQLKNELMR 3
            SC+SETLSTLRFAQRAKAI+NKAVVN ETTE DVN LR QIRQLK+EL+R
Sbjct: 374 QSCKSETLSTLRFAQRAKAIQNKAVVN-ETTEDDVNVLREQIRQLKDELIR 423


>KNA24096.1 hypothetical protein SOVF_019010 [Spinacia oleracea]
          Length = 1271

 Score =  187 bits (475), Expect = 6e-53
 Identities = 96/111 (86%), Positives = 102/111 (91%)
 Frame = -1

Query: 335 AGNINRSLSQLGNLIKILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMICSISPA 156
           AGNINRSLSQLGNLI ILAE+SQTGKQRHIPYRDSRLTFLLQESLGGNAK+AMIC+ISPA
Sbjct: 363 AGNINRSLSQLGNLINILAEISQTGKQRHIPYRDSRLTFLLQESLGGNAKMAMICAISPA 422

Query: 155 ASCRSETLSTLRFAQRAKAIKNKAVVNEETTESDVNFLRGQIRQLKNELMR 3
            SC+SET STLRFAQRAKAIKNKAVVNE   E DVNFLR  IRQLK+EL+R
Sbjct: 423 QSCKSETFSTLRFAQRAKAIKNKAVVNEVMQEDDVNFLREVIRQLKDELLR 473


>XP_010650335.1 PREDICTED: kinesin-like protein KIN-12B [Vitis vinifera]
          Length = 1376

 Score =  186 bits (473), Expect = 1e-52
 Identities = 97/111 (87%), Positives = 103/111 (92%)
 Frame = -1

Query: 335 AGNINRSLSQLGNLIKILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMICSISPA 156
           AGNINRSLSQLGNLI ILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAM+C+ISP 
Sbjct: 369 AGNINRSLSQLGNLINILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMVCAISPV 428

Query: 155 ASCRSETLSTLRFAQRAKAIKNKAVVNEETTESDVNFLRGQIRQLKNELMR 3
            SC+SETLSTLRFAQRAKAIKNKAVVN E  + DVNFLRG IRQLK+EL+R
Sbjct: 429 QSCKSETLSTLRFAQRAKAIKNKAVVN-EVMQDDVNFLRGVIRQLKDELLR 478


>CBI17294.3 unnamed protein product, partial [Vitis vinifera]
          Length = 1251

 Score =  186 bits (473), Expect = 1e-52
 Identities = 97/111 (87%), Positives = 103/111 (92%)
 Frame = -1

Query: 335 AGNINRSLSQLGNLIKILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMICSISPA 156
           AGNINRSLSQLGNLI ILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAM+C+ISP 
Sbjct: 369 AGNINRSLSQLGNLINILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMVCAISPV 428

Query: 155 ASCRSETLSTLRFAQRAKAIKNKAVVNEETTESDVNFLRGQIRQLKNELMR 3
            SC+SETLSTLRFAQRAKAIKNKAVVN E  + DVNFLRG IRQLK+EL+R
Sbjct: 429 QSCKSETLSTLRFAQRAKAIKNKAVVN-EVMQDDVNFLRGVIRQLKDELLR 478


>JAU77566.1 Kinesin-like protein KIN12B, partial [Noccaea caerulescens]
          Length = 437

 Score =  179 bits (454), Expect = 2e-52
 Identities = 92/111 (82%), Positives = 101/111 (90%)
 Frame = -1

Query: 335 AGNINRSLSQLGNLIKILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMICSISPA 156
           AGNINRSLSQLGNLI ILAE+SQTGKQRHIPYRDSRLTFLLQESLGGNAKLAM+C++SP+
Sbjct: 173 AGNINRSLSQLGNLINILAEISQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMVCAVSPS 232

Query: 155 ASCRSETLSTLRFAQRAKAIKNKAVVNEETTESDVNFLRGQIRQLKNELMR 3
            SCRSET STLRFAQRAKAI+NKAVVN E  + DVNFLR  IRQL++EL R
Sbjct: 233 QSCRSETFSTLRFAQRAKAIQNKAVVN-EVMQDDVNFLREVIRQLRDELQR 282


>OAE28312.1 hypothetical protein AXG93_2490s1000 [Marchantia polymorpha subsp.
           polymorpha]
          Length = 1572

 Score =  185 bits (469), Expect = 4e-52
 Identities = 95/111 (85%), Positives = 106/111 (95%)
 Frame = -1

Query: 335 AGNINRSLSQLGNLIKILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMICSISPA 156
           AGNIN+SLSQLGN+I ILA+V+QTGKQRHIPYRDSRLTFLLQESLGGNAKLAMIC+ISPA
Sbjct: 424 AGNINKSLSQLGNVINILADVAQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMICAISPA 483

Query: 155 ASCRSETLSTLRFAQRAKAIKNKAVVNEETTESDVNFLRGQIRQLKNELMR 3
           +SC++ETLSTLRFAQRAKA++NKAVVNEET  SDVN LR QIRQLK+ELMR
Sbjct: 484 SSCKNETLSTLRFAQRAKAVQNKAVVNEETA-SDVNLLREQIRQLKDELMR 533


>XP_010246668.1 PREDICTED: kinesin-like protein KIN-12B [Nelumbo nucifera]
          Length = 1393

 Score =  183 bits (465), Expect = 1e-51
 Identities = 97/111 (87%), Positives = 102/111 (91%)
 Frame = -1

Query: 335 AGNINRSLSQLGNLIKILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMICSISPA 156
           AGNINRSLSQLGNLI ILAEVSQTGK RHIPYRDSRLTFLLQESLGGNAKLAMIC+ISPA
Sbjct: 362 AGNINRSLSQLGNLINILAEVSQTGKHRHIPYRDSRLTFLLQESLGGNAKLAMICAISPA 421

Query: 155 ASCRSETLSTLRFAQRAKAIKNKAVVNEETTESDVNFLRGQIRQLKNELMR 3
            SC+SETLSTLRFAQRAKAIKNKAVVN E  + DVNFLR  IRQLK+EL+R
Sbjct: 422 QSCKSETLSTLRFAQRAKAIKNKAVVN-EVMQDDVNFLREVIRQLKDELLR 471


>OAY81494.1 Kinesin-like protein KIN12B [Ananas comosus]
          Length = 517

 Score =  178 bits (452), Expect = 1e-51
 Identities = 93/111 (83%), Positives = 102/111 (91%)
 Frame = -1

Query: 335 AGNINRSLSQLGNLIKILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMICSISPA 156
           AGNINRSLSQLGNLI ILAEVSQ+GK RHIPYRDS+LTFLLQESLGGNAKLAMIC+ISP+
Sbjct: 368 AGNINRSLSQLGNLINILAEVSQSGKHRHIPYRDSKLTFLLQESLGGNAKLAMICAISPS 427

Query: 155 ASCRSETLSTLRFAQRAKAIKNKAVVNEETTESDVNFLRGQIRQLKNELMR 3
            SC+SE+ STLRFAQRAKAIKNKAVVN E T+ DVN LR QIRQLK+EL+R
Sbjct: 428 QSCKSESFSTLRFAQRAKAIKNKAVVN-EITQDDVNVLREQIRQLKDELLR 477


>XP_010907239.1 PREDICTED: kinesin-like protein KIN-12B [Elaeis guineensis]
          Length = 1256

 Score =  183 bits (464), Expect = 2e-51
 Identities = 95/111 (85%), Positives = 105/111 (94%)
 Frame = -1

Query: 335 AGNINRSLSQLGNLIKILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMICSISPA 156
           AGNINRSLSQLGNLIKILAEVSQ+GKQRHIPYRDSRLTFLLQESLGGNAKLA++C+ISP+
Sbjct: 357 AGNINRSLSQLGNLIKILAEVSQSGKQRHIPYRDSRLTFLLQESLGGNAKLAILCAISPS 416

Query: 155 ASCRSETLSTLRFAQRAKAIKNKAVVNEETTESDVNFLRGQIRQLKNELMR 3
            SC++ETLSTLRFAQRAKAIKNKAVVN E T+ DVN LR QIRQLK+EL+R
Sbjct: 417 QSCKNETLSTLRFAQRAKAIKNKAVVN-EITQDDVNVLREQIRQLKDELLR 466


>XP_009415942.1 PREDICTED: kinesin-like protein KIN-12A [Musa acuminata subsp.
           malaccensis]
          Length = 1297

 Score =  182 bits (463), Expect = 2e-51
 Identities = 95/111 (85%), Positives = 103/111 (92%)
 Frame = -1

Query: 335 AGNINRSLSQLGNLIKILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMICSISPA 156
           AGNINRSLSQLGNLI ILAEVSQ+GKQRHIPYRDSRLTFLLQESLGGNAKLAMIC++SP+
Sbjct: 356 AGNINRSLSQLGNLINILAEVSQSGKQRHIPYRDSRLTFLLQESLGGNAKLAMICAVSPS 415

Query: 155 ASCRSETLSTLRFAQRAKAIKNKAVVNEETTESDVNFLRGQIRQLKNELMR 3
            SC+SET STLRFAQRAKAIKNKAVVN E T+ DVN LR QIRQLK+EL+R
Sbjct: 416 QSCKSETFSTLRFAQRAKAIKNKAVVN-EITQDDVNVLREQIRQLKDELLR 465


>XP_020112122.1 kinesin-like protein KIN-12A [Ananas comosus]
          Length = 1231

 Score =  182 bits (462), Expect = 3e-51
 Identities = 96/111 (86%), Positives = 103/111 (92%)
 Frame = -1

Query: 335 AGNINRSLSQLGNLIKILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMICSISPA 156
           AGNINRSLSQLGNLI ILAEVSQ+GKQRHIPYRDSRLTFLLQESLGGNAKLAMIC+ISP+
Sbjct: 377 AGNINRSLSQLGNLINILAEVSQSGKQRHIPYRDSRLTFLLQESLGGNAKLAMICAISPS 436

Query: 155 ASCRSETLSTLRFAQRAKAIKNKAVVNEETTESDVNFLRGQIRQLKNELMR 3
             C+SETLSTLRFAQRAKAIKNKAVVN E T+ DVN LR QIRQLK+EL+R
Sbjct: 437 QRCKSETLSTLRFAQRAKAIKNKAVVN-EITQDDVNVLREQIRQLKDELVR 486


>OAY69167.1 Kinesin-like protein KIN12B [Ananas comosus]
          Length = 1211

 Score =  182 bits (462), Expect = 3e-51
 Identities = 96/111 (86%), Positives = 103/111 (92%)
 Frame = -1

Query: 335 AGNINRSLSQLGNLIKILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMICSISPA 156
           AGNINRSLSQLGNLI ILAEVSQ+GKQRHIPYRDSRLTFLLQESLGGNAKLAMIC+ISP+
Sbjct: 357 AGNINRSLSQLGNLINILAEVSQSGKQRHIPYRDSRLTFLLQESLGGNAKLAMICAISPS 416

Query: 155 ASCRSETLSTLRFAQRAKAIKNKAVVNEETTESDVNFLRGQIRQLKNELMR 3
             C+SETLSTLRFAQRAKAIKNKAVVN E T+ DVN LR QIRQLK+EL+R
Sbjct: 417 QRCKSETLSTLRFAQRAKAIKNKAVVN-EITQDDVNVLREQIRQLKDELVR 466


>OAY71388.1 Kinesin-like protein KIN12B, partial [Ananas comosus]
          Length = 791

 Score =  181 bits (458), Expect = 5e-51
 Identities = 95/111 (85%), Positives = 102/111 (91%)
 Frame = -1

Query: 335 AGNINRSLSQLGNLIKILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMICSISPA 156
           AGNINRSLSQLGNLI ILAEVSQ+GKQRHIPYRDSRLTFLLQESLGGNAKLAMIC+ISP+
Sbjct: 357 AGNINRSLSQLGNLINILAEVSQSGKQRHIPYRDSRLTFLLQESLGGNAKLAMICAISPS 416

Query: 155 ASCRSETLSTLRFAQRAKAIKNKAVVNEETTESDVNFLRGQIRQLKNELMR 3
             C+SETL TLRFAQRAKAIKNKAVVN E T+ DVN LR QIRQLK+EL+R
Sbjct: 417 QRCKSETLGTLRFAQRAKAIKNKAVVN-EITQDDVNVLREQIRQLKDELVR 466


>JAU41170.1 Kinesin-like protein KIN12B, partial [Noccaea caerulescens]
          Length = 656

 Score =  179 bits (454), Expect = 6e-51
 Identities = 92/111 (82%), Positives = 101/111 (90%)
 Frame = -1

Query: 335 AGNINRSLSQLGNLIKILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMICSISPA 156
           AGNINRSLSQLGNLI ILAE+SQTGKQRHIPYRDSRLTFLLQESLGGNAKLAM+C++SP+
Sbjct: 147 AGNINRSLSQLGNLINILAEISQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMVCAVSPS 206

Query: 155 ASCRSETLSTLRFAQRAKAIKNKAVVNEETTESDVNFLRGQIRQLKNELMR 3
            SCRSET STLRFAQRAKAI+NKAVVN E  + DVNFLR  IRQL++EL R
Sbjct: 207 QSCRSETFSTLRFAQRAKAIQNKAVVN-EVMQDDVNFLREVIRQLRDELQR 256


>XP_010326953.1 PREDICTED: kinesin-like protein KIN-12B [Solanum lycopersicum]
          Length = 1367

 Score =  181 bits (460), Expect = 6e-51
 Identities = 96/111 (86%), Positives = 102/111 (91%)
 Frame = -1

Query: 335 AGNINRSLSQLGNLIKILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMICSISPA 156
           AGNINRSLSQLGNLI ILAEVSQTGK RHIPYRDS+LTFLLQESLGGNAKLAMIC+ISPA
Sbjct: 359 AGNINRSLSQLGNLINILAEVSQTGKHRHIPYRDSKLTFLLQESLGGNAKLAMICAISPA 418

Query: 155 ASCRSETLSTLRFAQRAKAIKNKAVVNEETTESDVNFLRGQIRQLKNELMR 3
            SC+SETLSTLRFAQRAKAIKNKAVVNEE  + DVN LR  IRQLK+EL+R
Sbjct: 419 QSCKSETLSTLRFAQRAKAIKNKAVVNEE-MQDDVNILREVIRQLKDELIR 468


>XP_015086149.1 PREDICTED: kinesin-like protein KIN12B [Solanum pennellii]
          Length = 1364

 Score =  181 bits (460), Expect = 6e-51
 Identities = 96/111 (86%), Positives = 102/111 (91%)
 Frame = -1

Query: 335 AGNINRSLSQLGNLIKILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMICSISPA 156
           AGNINRSLSQLGNLI ILAEVSQTGK RHIPYRDS+LTFLLQESLGGNAKLAMIC+ISPA
Sbjct: 359 AGNINRSLSQLGNLINILAEVSQTGKHRHIPYRDSKLTFLLQESLGGNAKLAMICAISPA 418

Query: 155 ASCRSETLSTLRFAQRAKAIKNKAVVNEETTESDVNFLRGQIRQLKNELMR 3
            SC+SETLSTLRFAQRAKAIKNKAVVNEE  + DVN LR  IRQLK+EL+R
Sbjct: 419 QSCKSETLSTLRFAQRAKAIKNKAVVNEE-MQDDVNILREVIRQLKDELIR 468


>XP_006358791.1 PREDICTED: kinesin-like protein KIN12B [Solanum tuberosum]
          Length = 1352

 Score =  181 bits (459), Expect = 8e-51
 Identities = 96/111 (86%), Positives = 102/111 (91%)
 Frame = -1

Query: 335 AGNINRSLSQLGNLIKILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMICSISPA 156
           AGNINRSLSQLGNLI ILAEVSQTGK RHIPYRDS+LTFLLQESLGGNAKLAMIC+ISPA
Sbjct: 359 AGNINRSLSQLGNLINILAEVSQTGKHRHIPYRDSKLTFLLQESLGGNAKLAMICAISPA 418

Query: 155 ASCRSETLSTLRFAQRAKAIKNKAVVNEETTESDVNFLRGQIRQLKNELMR 3
            SC+SETLSTLRFAQRAKAIKNKAVVNEE  + DVN LR  IRQLK+EL+R
Sbjct: 419 QSCKSETLSTLRFAQRAKAIKNKAVVNEE-MQDDVNVLREVIRQLKDELIR 468


>XP_016566233.1 PREDICTED: kinesin-like protein KIN12B [Capsicum annuum]
          Length = 1330

 Score =  181 bits (459), Expect = 8e-51
 Identities = 95/111 (85%), Positives = 102/111 (91%)
 Frame = -1

Query: 335 AGNINRSLSQLGNLIKILAEVSQTGKQRHIPYRDSRLTFLLQESLGGNAKLAMICSISPA 156
           AGNINRSLSQLGNLI ILAEVSQTGK RHIPYRDS+LTFLLQESLGGNAKLAMIC+ISPA
Sbjct: 368 AGNINRSLSQLGNLINILAEVSQTGKHRHIPYRDSKLTFLLQESLGGNAKLAMICAISPA 427

Query: 155 ASCRSETLSTLRFAQRAKAIKNKAVVNEETTESDVNFLRGQIRQLKNELMR 3
            SC+SETLSTLRFAQRAKAIKNKAV+NEE  + DVN LR  IRQLK+EL+R
Sbjct: 428 QSCKSETLSTLRFAQRAKAIKNKAVINEE-MQDDVNVLREYIRQLKDELIR 477