BLASTX nr result
ID: Ephedra29_contig00020543
seq
BLASTX 2.2.26 [Sep-21-2011]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Ephedra29_contig00020543
(978 letters)
Database: ./nr
115,041,592 sequences; 42,171,959,267 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
KVI05271.1 cytochrome P450 [Cynara cardunculus var. scolymus] 244 3e-73
XP_012077909.1 PREDICTED: cytochrome P450 86B1-like [Jatropha cu... 244 4e-73
XP_019163354.1 PREDICTED: cytochrome P450 86B1-like [Ipomoea nil] 243 2e-72
OAY37512.1 hypothetical protein MANES_11G107300 [Manihot esculenta] 242 3e-72
XP_010905515.1 PREDICTED: cytochrome P450 86B1-like [Elaeis guin... 241 4e-72
XP_008793701.1 PREDICTED: cytochrome P450 86B1 [Phoenix dactylif... 241 6e-72
XP_010250974.1 PREDICTED: cytochrome P450 86B1-like [Nelumbo nuc... 239 2e-71
EEF38624.1 cytochrome P450, putative [Ricinus communis] 239 2e-71
XP_019264365.1 PREDICTED: cytochrome P450 86B1-like [Nicotiana a... 239 3e-71
CDY34587.1 BnaC03g56870D [Brassica napus] 238 3e-71
XP_018449430.1 PREDICTED: cytochrome P450 86B1-like [Raphanus sa... 238 4e-71
XP_008796515.1 PREDICTED: cytochrome P450 86B1-like [Phoenix dac... 239 4e-71
XP_007043287.2 PREDICTED: cytochrome P450 86B1 [Theobroma cacao] 239 7e-71
EOX99118.1 Cytochrome P450, family 86, subfamily B, polypeptide ... 239 7e-71
XP_016501845.1 PREDICTED: cytochrome P450 86B1-like [Nicotiana t... 238 7e-71
XP_009794122.1 PREDICTED: cytochrome P450 86B1-like [Nicotiana s... 238 7e-71
XP_011022041.1 PREDICTED: cytochrome P450 86B1 [Populus euphratica] 238 1e-70
XP_011625110.1 PREDICTED: cytochrome P450 86B1 [Amborella tricho... 237 1e-70
XP_013674320.1 PREDICTED: cytochrome P450 86B1-like [Brassica na... 237 2e-70
XP_013603323.1 PREDICTED: cytochrome P450 86B1-like [Brassica ol... 237 2e-70
>KVI05271.1 cytochrome P450 [Cynara cardunculus var. scolymus]
Length = 540
Score = 244 bits (623), Expect = 3e-73
Identities = 121/289 (41%), Positives = 183/289 (63%), Gaps = 7/289 (2%)
Frame = +3
Query: 3 PTIVWKTIRALRLGTEGRLKVAREEAHGWLDRAMKCRVDVKYEVSDFREGRPMDLLSAMT 182
PT++WK +R L LG+E +LK + + + D ++ R K EV F + L S +
Sbjct: 244 PTLIWKIMRHLNLGSEKKLKQSIKGVDNFADEVIRTR---KKEV--FLQSEEKKLKSDLL 298
Query: 183 VQSPDLQEEQ-------HLKDTALSLILAGKDTVTSGLSWFFWAICRHPQVQQKVYEEVS 341
L++E+ L+D ++ ILAG+DT + LSWFFW + R+P V++++ E+
Sbjct: 299 TIFMGLKDEEGHPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDRNPTVEERILGEIC 358
Query: 342 AVAKRKPNVEPHETTQFSNQELKEMQYLQAALSETLRLYPPIPINIMEAAGDDVLPDGTF 521
+ K + + E F QE+K+M+YL AALSE+LRLYP +P++ E DDV PDGT
Sbjct: 359 QIVKDREDESESEDLIFRPQEVKKMEYLHAALSESLRLYPSVPVDHKEVVEDDVFPDGTI 418
Query: 522 IKKGTTLIYSVYAHGRNKELWGADAGEFRPERWMSDGKLYTNSEFKFMAFNAGPRRCLGR 701
+KKGT ++Y++YA GR + +WG D EFRPERW+ DG+ + S +KF AFN GPR CLG+
Sbjct: 419 LKKGTKVVYAIYAMGRMEAIWGKDCREFRPERWLRDGRFMSESAYKFTAFNGGPRLCLGK 478
Query: 702 DFAYWIMKCVAASVILRFKMRLASDRPVVTKLSFSLAMKDGLPITLEKR 848
DFAY+ MK AAS++ R+K+ + + PV KL+ ++ +K+GL + L KR
Sbjct: 479 DFAYYQMKFAAASILYRYKVTVVENHPVAPKLALTMYIKNGLKVKLSKR 527
>XP_012077909.1 PREDICTED: cytochrome P450 86B1-like [Jatropha curcas] KDP33058.1
hypothetical protein JCGZ_13644 [Jatropha curcas]
Length = 547
Score = 244 bits (623), Expect = 4e-73
Identities = 116/286 (40%), Positives = 181/286 (63%), Gaps = 4/286 (1%)
Frame = +3
Query: 3 PTIVWKTIRALRLGTEGRLKVAREEAHGWLDRAMKCRV-DVKYEVSDFREGRPMDLLSAM 179
PT +WK +R L LGTE +L+ + + + + ++ R ++ + + DLL+
Sbjct: 252 PTCIWKAMRYLDLGTERKLRASIKGVDEFAQQVIRTRKKELSLQTEYINNEQRSDLLTVF 311
Query: 180 TVQSPDLQE---EQHLKDTALSLILAGKDTVTSGLSWFFWAICRHPQVQQKVYEEVSAVA 350
+ ++ ++ L+D ++ ILAG+DT + L+WFFW + +H QV +K+ E+ +
Sbjct: 312 MGLKDENEQPFSDKFLRDICVNFILAGRDTSSVALTWFFWLLDQHEQVAEKILAEIYKII 371
Query: 351 KRKPNVEPHETTQFSNQELKEMQYLQAALSETLRLYPPIPINIMEAAGDDVLPDGTFIKK 530
+ + P F+ +E+K+M+YLQAALSE LRLYP +P++ E DDV PDGT +KK
Sbjct: 372 MEREHFNPETPLVFTPEEIKKMEYLQAALSEALRLYPSVPVDHKEIIEDDVFPDGTVLKK 431
Query: 531 GTTLIYSVYAHGRNKELWGADAGEFRPERWMSDGKLYTNSEFKFMAFNAGPRRCLGRDFA 710
GT +IY++Y GR + +WG D EF+PERW+ DG+ + S +KF AFN GPR CLG+DFA
Sbjct: 432 GTKVIYAIYTMGRMEAIWGKDCREFKPERWLKDGRFISESAYKFTAFNGGPRLCLGKDFA 491
Query: 711 YWIMKCVAASVILRFKMRLASDRPVVTKLSFSLAMKDGLPITLEKR 848
Y+ MK VAAS+I R+ + + D PV+ KL+ ++ MK+GL + L KR
Sbjct: 492 YYQMKFVAASIIYRYHVEVVKDHPVLPKLALTMYMKNGLKVNLRKR 537
>XP_019163354.1 PREDICTED: cytochrome P450 86B1-like [Ipomoea nil]
Length = 566
Score = 243 bits (620), Expect = 2e-72
Identities = 122/289 (42%), Positives = 183/289 (63%), Gaps = 7/289 (2%)
Frame = +3
Query: 3 PTIVWKTIRALRLGTEGRLKVAREEAHGWLDRAMKCRVDVKYEVSDFREGRPMDLLSAMT 182
PT +W+++RAL +GTE LK + ++ + D ++ R SD + R L M
Sbjct: 265 PTFIWRSMRALGVGTERGLKQSLKKVDEFADEVIRTRKKELSLASDGSKQRSDLLTVFMG 324
Query: 183 VQSPDLQ--EEQHLKDTALSLILAGKDTVTSGLSWFFWAICRHPQVQQKVYEEVSAVAKR 356
++ D + ++ L+D ++ ILAG+DT + LSWFFW + R P+V++++ E+ V
Sbjct: 325 LRGEDGRPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLNRKPEVEERILGEICQVLNS 384
Query: 357 KPN-----VEPHETTQFSNQELKEMQYLQAALSETLRLYPPIPINIMEAAGDDVLPDGTF 521
+ N E +T F +E+K+M YLQAALSE LRLYP +P++ E DDV PDGT
Sbjct: 385 RENGGSTNTENQDTLIFKPEEIKKMDYLQAALSEALRLYPSVPVDHKEVLEDDVFPDGTV 444
Query: 522 IKKGTTLIYSVYAHGRNKELWGADAGEFRPERWMSDGKLYTNSEFKFMAFNAGPRRCLGR 701
+KKGT +IY++YA GR + LWG D EF+PERW+ DG+ + S +KF AFN GPR CLG+
Sbjct: 445 LKKGTKVIYAIYAMGRMEGLWGKDCREFKPERWLRDGRFMSESAYKFTAFNGGPRLCLGK 504
Query: 702 DFAYWIMKCVAASVILRFKMRLASDRPVVTKLSFSLAMKDGLPITLEKR 848
DFAY+ MK AAS++ R+ +++ + PV K++ +L MK GL +TL +R
Sbjct: 505 DFAYYQMKFAAASILYRYHVKVVENHPVKPKMALTLYMKYGLKVTLSRR 553
>OAY37512.1 hypothetical protein MANES_11G107300 [Manihot esculenta]
Length = 543
Score = 242 bits (617), Expect = 3e-72
Identities = 116/285 (40%), Positives = 182/285 (63%), Gaps = 3/285 (1%)
Frame = +3
Query: 3 PTIVWKTIRALRLGTEGRLKVAREEAHGWLDRAMKCRV-DVKYEVSDFREGRPMDLLSAM 179
PT +WK +R L LG+E +LK + + + + +K R ++ + SD ++ R L M
Sbjct: 251 PTCIWKAMRYLDLGSEKKLKRSIKGVDEFAENVIKTRKKELSVQSSDDKKQRSDLLTVFM 310
Query: 180 TVQSPDLQ--EEQHLKDTALSLILAGKDTVTSGLSWFFWAICRHPQVQQKVYEEVSAVAK 353
++ + Q ++ L+D ++ ILAG+DT + LSWFFW + ++P ++ K+ E+ + K
Sbjct: 311 GLKDENGQPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDQNPHIEDKILAEICKIVK 370
Query: 354 RKPNVEPHETTQFSNQELKEMQYLQAALSETLRLYPPIPINIMEAAGDDVLPDGTFIKKG 533
+ + +P F +E+K+M YLQAALSE LRLYP +P++ E DD+ PDG+ +KKG
Sbjct: 371 EREDFDPKTPLVFRPEEIKKMDYLQAALSEALRLYPSVPVDHKEVVEDDIFPDGSLLKKG 430
Query: 534 TTLIYSVYAHGRNKELWGADAGEFRPERWMSDGKLYTNSEFKFMAFNAGPRRCLGRDFAY 713
T +IY++Y GR + +WG D EF+PERW+ DG+ + S +KF AFN GPR CLG+DFAY
Sbjct: 431 TKVIYAIYTMGRMEAIWGKDCREFKPERWLKDGRFMSESAYKFTAFNGGPRLCLGKDFAY 490
Query: 714 WIMKCVAASVILRFKMRLASDRPVVTKLSFSLAMKDGLPITLEKR 848
+ MK AAS+I R+ +++ D PV KL+ ++ MK GL + L +R
Sbjct: 491 YQMKFAAASIIHRYHVKVVKDHPVGPKLALTMYMKHGLKVNLSRR 535
>XP_010905515.1 PREDICTED: cytochrome P450 86B1-like [Elaeis guineensis]
Length = 545
Score = 241 bits (616), Expect = 4e-72
Identities = 115/289 (39%), Positives = 185/289 (64%), Gaps = 7/289 (2%)
Frame = +3
Query: 3 PTIVWKTIRALRLGTEGRLKVAREEAHGWLDRAMKCRV-DVKYEVSDFREGRPMDLLSAM 179
PT +WK +R LG E RL+ + ++ + ++ R ++ E D ++ DLL+
Sbjct: 250 PTAIWKAMRFFDLGNERRLRRSLKQVDEFAYEVIRTRKKELSLESGDEKKAARSDLLTVF 309
Query: 180 TVQSPD---LQEEQHLKDTALSLILAGKDTVTSGLSWFFWAICRHPQVQQKVYEEVSAVA 350
T + ++ L+D ++ ILAG+DT + L+WFFW + RHP+V+ K+ EE+ +
Sbjct: 310 TNYKDEEGMAFSDKFLRDVCVNFILAGRDTSSVALAWFFWLLNRHPEVEDKILEEIRRIV 369
Query: 351 KRKPNV---EPHETTQFSNQELKEMQYLQAALSETLRLYPPIPINIMEAAGDDVLPDGTF 521
+ + N E ++ F ++E+K M+YL AALSE LRLYP +P++ E D+V PDGT
Sbjct: 370 EERENNREGEGEKSLVFKSEEVKRMEYLHAALSEALRLYPSVPVDHKEVIEDEVFPDGTV 429
Query: 522 IKKGTTLIYSVYAHGRNKELWGADAGEFRPERWMSDGKLYTNSEFKFMAFNAGPRRCLGR 701
+KKGT +IY++Y+ GR + +WG + +++PERW+ DG+ + S +KF AFN GPR CLG+
Sbjct: 430 LKKGTKVIYAIYSMGRMESIWGKNCRDYKPERWLKDGRFMSESAYKFTAFNGGPRLCLGK 489
Query: 702 DFAYWIMKCVAASVILRFKMRLASDRPVVTKLSFSLAMKDGLPITLEKR 848
DFAY+ MK VAAS++ R+++++ PVV KL+ ++ MK GL +TL +R
Sbjct: 490 DFAYYQMKFVAASILYRYQVKVVESHPVVPKLALTMYMKYGLKVTLARR 538
>XP_008793701.1 PREDICTED: cytochrome P450 86B1 [Phoenix dactylifera]
Length = 531
Score = 241 bits (614), Expect = 6e-72
Identities = 124/296 (41%), Positives = 186/296 (62%), Gaps = 14/296 (4%)
Frame = +3
Query: 3 PTIVWKTIRALRLGTEGRLKVAREEAHGWLDRAMKCRVDVKYEVSDFRE-GRPMDLLSAM 179
P VWK ++ L +G+E RLKVA H + ++ + D + E+ + + DLLS +
Sbjct: 234 PPFVWKIMKHLDVGSEKRLKVAIRTVHTFAEKTVS---DRRAELRKLKGLDKRSDLLSRL 290
Query: 180 TVQSPDLQEEQH-----------LKDTALSLILAGKDTVTSGLSWFFWAICRHPQVQQKV 326
++E H LKD +S ILAG+DT + L+WFFW + +HPQV++++
Sbjct: 291 --MEAGVKEGGHGGMECNFSDKLLKDFCISFILAGRDTSSVALAWFFWLLHQHPQVEERI 348
Query: 327 YEEVSAVAKRKPN--VEPHETTQFSNQELKEMQYLQAALSETLRLYPPIPINIMEAAGDD 500
+E+S KR+ + P + F+ +ELK M+YLQAALSE+LRLYP +P++ E DD
Sbjct: 349 LDEISETIKRRQDEVCRPDDNIVFTVEELKRMEYLQAALSESLRLYPSVPLDFKEVLEDD 408
Query: 501 VLPDGTFIKKGTTLIYSVYAHGRNKELWGADAGEFRPERWMSDGKLYTNSEFKFMAFNAG 680
+LPDG+ +KKG + YS+Y+ R + +WG D EFRPERW+ DG L T S+FK+ FNAG
Sbjct: 409 ILPDGSVVKKGARVFYSIYSMARMERIWGKDCREFRPERWIKDGILVTESQFKYTVFNAG 468
Query: 681 PRRCLGRDFAYWIMKCVAASVILRFKMRLASDRPVVTKLSFSLAMKDGLPITLEKR 848
PR C+G+ FAY MK VAAS++LR+++ + + V KL+ +L MK+GL +T +R
Sbjct: 469 PRLCVGKKFAYMQMKMVAASILLRYRVEVVEGQEVKPKLTTTLYMKNGLRVTFSRR 524
>XP_010250974.1 PREDICTED: cytochrome P450 86B1-like [Nelumbo nucifera]
Length = 535
Score = 239 bits (610), Expect = 2e-71
Identities = 123/290 (42%), Positives = 188/290 (64%), Gaps = 8/290 (2%)
Frame = +3
Query: 3 PTIVWKTIRALRLGTEGRLKVAREEAHGWLDRAMKCRVDVKYEVSDFRE-GRPMDLLSAM 179
P VWK ++ L +G+E +LK A + H + ++ + R + E+S DLLS +
Sbjct: 243 PPFVWKFMKLLGIGSEKKLKEAVQIVHKFGEKTVTGR---RVELSKLGSLNDRSDLLSRL 299
Query: 180 T-VQSPDLQ-----EEQHLKDTALSLILAGKDTVTSGLSWFFWAICRHPQVQQKVYEEVS 341
V+ P+ + ++ L+D +S ILAG+DT + L+WFFW + +HP+V++++ +E+
Sbjct: 300 IEVEDPEQKGKKKFSDKFLEDFCISFILAGRDTSSVALTWFFWLLHKHPRVEERIIDEIK 359
Query: 342 A-VAKRKPNVEPHETTQFSNQELKEMQYLQAALSETLRLYPPIPINIMEAAGDDVLPDGT 518
V +RK E E FS ELK+M YLQAALSE+LRLYP +P++ E DDV PDG
Sbjct: 360 EIVGQRKSKKEKDEDVIFSIDELKKMVYLQAALSESLRLYPSVPMDFKEVQEDDVFPDGA 419
Query: 519 FIKKGTTLIYSVYAHGRNKELWGADAGEFRPERWMSDGKLYTNSEFKFMAFNAGPRRCLG 698
F+KKG + Y +Y+ GR +++WG D EF+PERWM +G+L + ++FK+ FNAGPR C+G
Sbjct: 420 FVKKGARVFYLIYSMGRMEDIWGKDCKEFKPERWMKNGELVSENQFKYAVFNAGPRLCVG 479
Query: 699 RDFAYWIMKCVAASVILRFKMRLASDRPVVTKLSFSLAMKDGLPITLEKR 848
+ FAY MK VAAS++LRF +++ + +V KL+ +L MK+GL +T E R
Sbjct: 480 KKFAYMQMKMVAASILLRFSVKVVEGQTIVPKLTTTLYMKNGLLVTFEPR 529
>EEF38624.1 cytochrome P450, putative [Ricinus communis]
Length = 550
Score = 239 bits (611), Expect = 2e-71
Identities = 117/288 (40%), Positives = 185/288 (64%), Gaps = 6/288 (2%)
Frame = +3
Query: 3 PTIVWKTIRALRLGTEGRLKVAREEAHGWLDRAMKCRV-DVKYEVSDFREGRPMDLLSA- 176
PT +WK ++ L LG E +LK + + + ++ ++ R ++ SD + D+L+
Sbjct: 254 PTCIWKAMKYLDLGAEKKLKGSIKAVDEFAEKVIRTRKKEMSSTTSDDDKKHGSDILTVF 313
Query: 177 --MTVQSPDLQEEQHLKDTALSLILAGKDTVTSGLSWFFWAICRHPQVQQKVYEEVSAVA 350
+ ++ ++ L+D ++ ILAG+DT + LSWFFW + +HP+V++K+ E+ +
Sbjct: 314 MRLKDENGKPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLDKHPKVEEKILAEIYKIV 373
Query: 351 KRKPN--VEPHETTQFSNQELKEMQYLQAALSETLRLYPPIPINIMEAAGDDVLPDGTFI 524
K++ +P F +E+K+M+YLQAALSE LRLYP +P++ E DD+ PDGT +
Sbjct: 374 KQRSEDPFDPKSPLMFRPEEVKKMEYLQAALSEALRLYPSVPVDHKEVIEDDIFPDGTRL 433
Query: 525 KKGTTLIYSVYAHGRNKELWGADAGEFRPERWMSDGKLYTNSEFKFMAFNAGPRRCLGRD 704
K+GT +IY++YA GR + +WG D EF+PERW+ DG+ + S +KF AFN GPR CLG+D
Sbjct: 434 KQGTKVIYAIYAMGRMEAIWGKDCREFKPERWLRDGRYMSESAYKFTAFNGGPRLCLGKD 493
Query: 705 FAYWIMKCVAASVILRFKMRLASDRPVVTKLSFSLAMKDGLPITLEKR 848
FAY+ MK AAS+I R+ +++A D VV KL+ ++ MK GL + L KR
Sbjct: 494 FAYYQMKFAAASIIYRYHVKVAKDHSVVPKLALTMYMKHGLKVNLFKR 541
>XP_019264365.1 PREDICTED: cytochrome P450 86B1-like [Nicotiana attenuata] OIT36479.1
cytochrome p450 86b1 [Nicotiana attenuata]
Length = 542
Score = 239 bits (610), Expect = 3e-71
Identities = 120/290 (41%), Positives = 182/290 (62%), Gaps = 8/290 (2%)
Frame = +3
Query: 3 PTIVWKTIRALRLGTEGRLKVAREEAHGWLDRAMKCRVDVKYEVSDFREGRPMDLLSAMT 182
PT+VWKT+R L LGTE LK + + + D +K R + + D + DLL+
Sbjct: 257 PTLVWKTMRWLGLGTERTLKHSLRKVDEFADEVIKTRKK-ELSLEDESSKKRSDLLTVFM 315
Query: 183 VQSPDLQEEQ-------HLKDTALSLILAGKDTVTSGLSWFFWAICRHPQVQQKVYEEVS 341
L++EQ L+D ++ ILAG+DT + LSWFFW + R+P+V++++ E+S
Sbjct: 316 ----GLRDEQGRPFSGKFLRDICVNFILAGRDTSSVALSWFFWLLNRNPEVEERILAEIS 371
Query: 342 AVAKRKPNVEPHETTQ-FSNQELKEMQYLQAALSETLRLYPPIPINIMEAAGDDVLPDGT 518
+ + ET F +E+K+M+YLQAALSE LRLYP +P++ E DDV PDGT
Sbjct: 372 KILNERSEDAIKETPLIFKPKEIKQMEYLQAALSEALRLYPSVPVDHKEVVEDDVFPDGT 431
Query: 519 FIKKGTTLIYSVYAHGRNKELWGADAGEFRPERWMSDGKLYTNSEFKFMAFNAGPRRCLG 698
+KKGT ++Y++Y GR + +WG D E++PERW+ DG+ T S ++F AFN GPR CLG
Sbjct: 432 VLKKGTKVVYAIYTMGRMEGIWGKDCREYKPERWLRDGRYMTESAYRFTAFNGGPRLCLG 491
Query: 699 RDFAYWIMKCVAASVILRFKMRLASDRPVVTKLSFSLAMKDGLPITLEKR 848
+DFAY+ MK VAAS++ R+ +++ PV K++ ++ +K GL + L +R
Sbjct: 492 KDFAYYQMKFVAASILYRYNVKVVEGHPVTPKMALTMYLKYGLQVKLSRR 541
>CDY34587.1 BnaC03g56870D [Brassica napus]
Length = 515
Score = 238 bits (608), Expect = 3e-71
Identities = 117/284 (41%), Positives = 182/284 (64%), Gaps = 2/284 (0%)
Frame = +3
Query: 3 PTIVWKTIRALRLGTEGRLKVAREEAHGWLDRAMKC--RVDVKYEVSDFREGRPMDLLSA 176
P +WK ++ L +G E L+ A + HG++++ + R DV + + + +
Sbjct: 230 PPFIWKPMKFLDIGYEKGLRKAIDVVHGFVNKMIMDHNRSDVLTRIIQIENNKKGNKIGP 289
Query: 177 MTVQSPDLQEEQHLKDTALSLILAGKDTVTSGLSWFFWAICRHPQVQQKVYEEVSAVAKR 356
T++ + S ILAG+DT + +SWFFW I RHPQV+ K+ +E+ + K+
Sbjct: 290 STIR--------FFRQFCTSFILAGRDTSSVAISWFFWVIQRHPQVENKIIQEIRQIVKQ 341
Query: 357 KPNVEPHETTQFSNQELKEMQYLQAALSETLRLYPPIPINIMEAAGDDVLPDGTFIKKGT 536
+ +P +++ F+ +EL M YLQAA+SETLRLYPPIP+ + +A DD+ PDGTFIKKG+
Sbjct: 342 RG--DPSDSSLFTVKELNNMVYLQAAISETLRLYPPIPMEMKQAIEDDMFPDGTFIKKGS 399
Query: 537 TLIYSVYAHGRNKELWGADAGEFRPERWMSDGKLYTNSEFKFMAFNAGPRRCLGRDFAYW 716
+ +S+YA GR + +WG D EFRPERW+ GK ++ ++K++ FNAGPR CLG+ FAY
Sbjct: 400 RVYFSIYAMGRMETIWGKDCEEFRPERWIQAGKFVSDDQYKYVVFNAGPRLCLGKTFAYL 459
Query: 717 IMKCVAASVILRFKMRLASDRPVVTKLSFSLAMKDGLPITLEKR 848
MK +AASV+LR+ +++A D VV +++ +L MK GL +T+ R
Sbjct: 460 QMKMIAASVLLRYSVKVAQDHVVVPRVTTNLYMKYGLKVTITPR 503
>XP_018449430.1 PREDICTED: cytochrome P450 86B1-like [Raphanus sativus]
Length = 526
Score = 238 bits (608), Expect = 4e-71
Identities = 119/288 (41%), Positives = 184/288 (63%), Gaps = 6/288 (2%)
Frame = +3
Query: 3 PTIVWKTIRALRLGTEGRLKVAREEAHGWLDRAMKCRVDVKYEVSDFREGRPMDLLSAMT 182
P +WK ++ L +G E L+ A + HG++++ + R+ + + + + R L +
Sbjct: 230 PPFIWKPMKFLNIGYEKSLRNAIDVVHGFVNKMIMDRICMLKD-DETLDNRSDVLTRIIQ 288
Query: 183 VQSPDLQEE------QHLKDTALSLILAGKDTVTSGLSWFFWAICRHPQVQQKVYEEVSA 344
+ S E + + S ILAG+DT + LSWFFW I RHPQV+ K+ E+
Sbjct: 289 IASHKKGNEIGPSTIRFFRQFCTSFILAGRDTSSVALSWFFWVIQRHPQVENKIIHEIRE 348
Query: 345 VAKRKPNVEPHETTQFSNQELKEMQYLQAALSETLRLYPPIPINIMEAAGDDVLPDGTFI 524
+ K++ +P +++ F+ +EL M YLQAA+SETLRLYPPIP+ + +A GDDV PDGTFI
Sbjct: 349 IMKQRG--DPSDSSLFTVRELNNMVYLQAAISETLRLYPPIPMEMKQAIGDDVFPDGTFI 406
Query: 525 KKGTTLIYSVYAHGRNKELWGADAGEFRPERWMSDGKLYTNSEFKFMAFNAGPRRCLGRD 704
KKG+ + +S+YA GR + +WG D FRPERW+ GK ++ +FK++ FNAGPR C+G+
Sbjct: 407 KKGSRVYFSIYAMGRMESIWGKDCEMFRPERWIQGGKFVSDDQFKYVVFNAGPRLCIGKT 466
Query: 705 FAYWIMKCVAASVILRFKMRLASDRPVVTKLSFSLAMKDGLPITLEKR 848
FAY MK +AASV+L++ +++A D VV +++ +L MK GL +T+ R
Sbjct: 467 FAYLQMKMIAASVLLKYSIKVAQDHVVVPRVTTNLYMKYGLKVTITPR 514
>XP_008796515.1 PREDICTED: cytochrome P450 86B1-like [Phoenix dactylifera]
Length = 557
Score = 239 bits (610), Expect = 4e-71
Identities = 114/289 (39%), Positives = 184/289 (63%), Gaps = 7/289 (2%)
Frame = +3
Query: 3 PTIVWKTIRALRLGTEGRLKVAREEAHGWLDRAMKCRV-DVKYEVSDFREGRPMDLLSAM 179
PT +WK +R LG E RL+ + + + ++ R ++ E D ++ DLL+A
Sbjct: 250 PTAIWKAMRFFDLGNERRLRRSLKHVDDFAYEVIRTRKKELSLESGDEKKAARSDLLTAF 309
Query: 180 TVQSPD---LQEEQHLKDTALSLILAGKDTVTSGLSWFFWAICRHPQVQQKVYEEVSAVA 350
T D ++ L+D ++ ILAG+DT + L+WFFW + ++P+V++++ EEV +
Sbjct: 310 TSHKDDHGTAFSDKLLRDVCVNFILAGRDTSSVALAWFFWLLNQYPEVEKRILEEVRRIV 369
Query: 351 KRKP---NVEPHETTQFSNQELKEMQYLQAALSETLRLYPPIPINIMEAAGDDVLPDGTF 521
+ + E E+ F +E+K M+YLQAAL+E LRLYP +P++ E D+V PDGT
Sbjct: 370 EDREYNREEEGEESLVFKREEVKRMEYLQAALTEALRLYPSVPVDHKEVIEDEVFPDGTV 429
Query: 522 IKKGTTLIYSVYAHGRNKELWGADAGEFRPERWMSDGKLYTNSEFKFMAFNAGPRRCLGR 701
+KKGT ++Y++Y+ GR + +WG D +++PERW+ DG+ + S +KF AFN GPR CLG+
Sbjct: 430 LKKGTKVVYAIYSMGRMESIWGKDCRDYKPERWLKDGRFMSESAYKFTAFNGGPRLCLGK 489
Query: 702 DFAYWIMKCVAASVILRFKMRLASDRPVVTKLSFSLAMKDGLPITLEKR 848
DFAY+ MK VAAS++ R+ +++ PVV KL+ ++ M+ GL +TL +R
Sbjct: 490 DFAYYQMKFVAASILYRYHVKVVESHPVVPKLALTMYMRYGLKVTLSRR 538
>XP_007043287.2 PREDICTED: cytochrome P450 86B1 [Theobroma cacao]
Length = 565
Score = 239 bits (609), Expect = 7e-71
Identities = 121/289 (41%), Positives = 184/289 (63%), Gaps = 7/289 (2%)
Frame = +3
Query: 3 PTIVWKTIRALRLGTEGRLKVAREEAHGWLDRAMKCRVDVKYEVSDFREGRPMDLLSAMT 182
PT +WK +R L LGTE +LK + + + + ++ R S+ ++ R DLL+
Sbjct: 266 PTCIWKAMRYLDLGTEKKLKRSIKGVDKFAEEVIRTRKKELALQSEDKKQRS-DLLTIF- 323
Query: 183 VQSPDLQ----EEQHLKDTALSLILAGKDTVTSGLSWFFWAICRHPQVQQKVYEEVSAVA 350
++ D Q ++ L+D ++ ILAG+DT + LSWFFW + ++P V++K+ E+S +
Sbjct: 324 MRLKDEQGKPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLEKNPMVEEKILAEISRII 383
Query: 351 KRKPNVEPHETTQ---FSNQELKEMQYLQAALSETLRLYPPIPINIMEAAGDDVLPDGTF 521
+ ++ E F +E+K+M YLQAALSE LRLYP +P++ E DDV PDGT
Sbjct: 384 NEREEMKDEEAKSRLVFRPEEIKKMDYLQAALSEALRLYPSVPVDHKEVVEDDVFPDGTV 443
Query: 522 IKKGTTLIYSVYAHGRNKELWGADAGEFRPERWMSDGKLYTNSEFKFMAFNAGPRRCLGR 701
+KKGT +IY++YA GR + +WG D E++PERW+ DG+ + S +KF AFN GPR CLG+
Sbjct: 444 LKKGTKVIYAIYAMGRMEAIWGKDCREYKPERWLRDGRYMSESAYKFTAFNGGPRLCLGK 503
Query: 702 DFAYWIMKCVAASVILRFKMRLASDRPVVTKLSFSLAMKDGLPITLEKR 848
DFAY+ MK AAS+I R+++++ D PV KL+ ++ MK GL + L KR
Sbjct: 504 DFAYYQMKFAAASIIYRYRVKVVKDHPVAPKLALTMYMKHGLNVNLIKR 552
>EOX99118.1 Cytochrome P450, family 86, subfamily B, polypeptide 1 isoform 1
[Theobroma cacao] EOX99119.1 Cytochrome P450, family 86,
subfamily B, polypeptide 1 isoform 1 [Theobroma cacao]
EOX99120.1 Cytochrome P450, family 86, subfamily B,
polypeptide 1 isoform 1 [Theobroma cacao]
Length = 565
Score = 239 bits (609), Expect = 7e-71
Identities = 121/289 (41%), Positives = 184/289 (63%), Gaps = 7/289 (2%)
Frame = +3
Query: 3 PTIVWKTIRALRLGTEGRLKVAREEAHGWLDRAMKCRVDVKYEVSDFREGRPMDLLSAMT 182
PT +WK +R L LGTE +LK + + + + ++ R S+ ++ R DLL+
Sbjct: 266 PTCIWKAMRYLDLGTEKKLKRSIKGVDKFAEEVIRTRKKELALQSEDKKQRS-DLLTIF- 323
Query: 183 VQSPDLQ----EEQHLKDTALSLILAGKDTVTSGLSWFFWAICRHPQVQQKVYEEVSAVA 350
++ D Q ++ L+D ++ ILAG+DT + LSWFFW + ++P V++K+ E+S +
Sbjct: 324 MRLKDEQGKPFSDKFLRDICVNFILAGRDTSSVALSWFFWLLEKNPMVEEKILAEISRII 383
Query: 351 KRKPNVEPHETTQ---FSNQELKEMQYLQAALSETLRLYPPIPINIMEAAGDDVLPDGTF 521
+ ++ E F +E+K+M YLQAALSE LRLYP +P++ E DDV PDGT
Sbjct: 384 NEREEMKDEEAKSRLVFRPEEIKKMDYLQAALSEALRLYPSVPVDHKEVVEDDVFPDGTV 443
Query: 522 IKKGTTLIYSVYAHGRNKELWGADAGEFRPERWMSDGKLYTNSEFKFMAFNAGPRRCLGR 701
+KKGT +IY++YA GR + +WG D E++PERW+ DG+ + S +KF AFN GPR CLG+
Sbjct: 444 LKKGTKVIYAIYAMGRMEAIWGKDCREYKPERWLRDGRYMSESAYKFTAFNGGPRLCLGK 503
Query: 702 DFAYWIMKCVAASVILRFKMRLASDRPVVTKLSFSLAMKDGLPITLEKR 848
DFAY+ MK AAS+I R+++++ D PV KL+ ++ MK GL + L KR
Sbjct: 504 DFAYYQMKFAAASIIYRYRVKVVKDHPVAPKLALTMYMKHGLNVNLIKR 552
>XP_016501845.1 PREDICTED: cytochrome P450 86B1-like [Nicotiana tabacum]
Length = 551
Score = 238 bits (608), Expect = 7e-71
Identities = 116/289 (40%), Positives = 179/289 (61%), Gaps = 7/289 (2%)
Frame = +3
Query: 3 PTIVWKTIRALRLGTEGRLKVAREEAHGWLDRAMKCRVDVKYEVSDFREGRPMDLLSAMT 182
PT+VWKT+R L LGTE LK + ++ + D +K R S + DLL+
Sbjct: 266 PTLVWKTMRCLGLGTERTLKHSLKKVDEFADEVIKTRKKELSLASSEDSKKRSDLLTVFM 325
Query: 183 VQSPDLQEEQH-------LKDTALSLILAGKDTVTSGLSWFFWAICRHPQVQQKVYEEVS 341
L++EQ L+D ++ ILAG+DT + LSWFFW + R+P+V++++ E+
Sbjct: 326 ----GLRDEQGRPFSGKILRDICVNFILAGRDTSSVALSWFFWLLNRNPEVEERILAEIC 381
Query: 342 AVAKRKPNVEPHETTQFSNQELKEMQYLQAALSETLRLYPPIPINIMEAAGDDVLPDGTF 521
+ + + F +E+K+M+YLQAALSE LRLYP +P++ E DDV PDGT
Sbjct: 382 KILNEREDAIKESPLIFKPKEIKQMEYLQAALSEALRLYPSVPVDHKEVVEDDVFPDGTV 441
Query: 522 IKKGTTLIYSVYAHGRNKELWGADAGEFRPERWMSDGKLYTNSEFKFMAFNAGPRRCLGR 701
+KKGT ++Y++Y GR + +WG D E++PERW+ DG+ + S ++F AFN GPR CLG+
Sbjct: 442 LKKGTKVVYAIYTMGRMEGIWGKDCREYKPERWLRDGRYMSESAYRFTAFNGGPRLCLGK 501
Query: 702 DFAYWIMKCVAASVILRFKMRLASDRPVVTKLSFSLAMKDGLPITLEKR 848
DFAY+ MK VAAS++ R+ +++ PV K++ ++ +K GL + L +R
Sbjct: 502 DFAYYQMKFVAASILYRYNVKVVEGHPVTPKMALTMYLKYGLQVKLSRR 550
>XP_009794122.1 PREDICTED: cytochrome P450 86B1-like [Nicotiana sylvestris]
XP_016443125.1 PREDICTED: cytochrome P450 86B1-like
[Nicotiana tabacum]
Length = 537
Score = 238 bits (607), Expect = 7e-71
Identities = 119/290 (41%), Positives = 184/290 (63%), Gaps = 8/290 (2%)
Frame = +3
Query: 3 PTIVWKTIRALRLGTEGRLKVAREEAHGWLDRAMKCRVDVKYEVSDFREGRPMDLLSAMT 182
PT+VWKT+R L LGTE LK + ++ + + D +K R + + D + DLL+
Sbjct: 252 PTLVWKTMRWLGLGTERTLKHSLKKVNEFADEVIKTRKK-ELSLEDESSKKRSDLLTVFM 310
Query: 183 VQSPDLQEEQH-------LKDTALSLILAGKDTVTSGLSWFFWAICRHPQVQQKVYEEVS 341
L++EQ L+D ++ ILAG+DT + LSWFFW + R+P+V++++ E+S
Sbjct: 311 ----GLRDEQGKPFSGKLLRDICVNFILAGRDTSSVALSWFFWLLNRNPEVEERILAEIS 366
Query: 342 AVA-KRKPNVEPHETTQFSNQELKEMQYLQAALSETLRLYPPIPINIMEAAGDDVLPDGT 518
+ +R + F +E+K+M+YLQAALSE LRLYP +P++ E DDV PDGT
Sbjct: 367 KILNERSEDAIKESPLIFKPKEIKQMEYLQAALSEALRLYPSVPVDHKEVVEDDVFPDGT 426
Query: 519 FIKKGTTLIYSVYAHGRNKELWGADAGEFRPERWMSDGKLYTNSEFKFMAFNAGPRRCLG 698
+KKGT ++Y++Y GR + +WG D E++PERW+ DG+ T S ++F AFN GPR CLG
Sbjct: 427 VLKKGTKVVYAIYTMGRMEGIWGEDCREYKPERWLRDGRYMTESAYRFTAFNGGPRLCLG 486
Query: 699 RDFAYWIMKCVAASVILRFKMRLASDRPVVTKLSFSLAMKDGLPITLEKR 848
+DFAY+ MK VAAS++ R+ +++ PV K++ ++ +K GL + L +R
Sbjct: 487 KDFAYYQMKFVAASILYRYNVKVVEGHPVSPKMALTMYLKYGLQVKLSRR 536
>XP_011022041.1 PREDICTED: cytochrome P450 86B1 [Populus euphratica]
Length = 566
Score = 238 bits (608), Expect = 1e-70
Identities = 115/288 (39%), Positives = 183/288 (63%), Gaps = 6/288 (2%)
Frame = +3
Query: 3 PTIVWKTIRALRLGTEGRLKVAREEAHGWLDRAMKCRV-DVKYEVSDFREGRPMDLLSA- 176
PT +WK +R L LG+E +LK + ++ + + ++ R ++ + D ++ + DLL+
Sbjct: 267 PTCIWKAMRYLDLGSEKKLKRSIKDVDEFAEDVIRTRKKELSIQSEDDKKKQGSDLLTVF 326
Query: 177 --MTVQSPDLQEEQHLKDTALSLILAGKDTVTSGLSWFFWAICRHPQVQQKVYEEVSAVA 350
+ ++ ++ L+D ++ ILAG+DT + +SWFFW + HP V++K+ E+ +
Sbjct: 327 MGLKDENGKPFSDRFLRDICVNFILAGRDTSSVAMSWFFWLLDSHPTVEEKILAEICKIV 386
Query: 351 KRKPNVEPHETTQFSNQELKEMQYLQAALSETLRLYPPIPINIMEAAGDDVLPDGTFIKK 530
+ ++ FS QE+K+M YLQAALSE LRLYP +P++ E DD+ PDGT ++K
Sbjct: 387 SEREELDTKTPLVFSPQEIKKMNYLQAALSEALRLYPSVPVDHKEVVEDDIFPDGTVLEK 446
Query: 531 GTTLIYSVYAHGRNKELWGADAGEFRPERW--MSDGKLYTNSEFKFMAFNAGPRRCLGRD 704
GT +IY++YA GR + +WG+D EF+PERW M DG+ + S +KF AFN GPR CLG+D
Sbjct: 447 GTKVIYAIYAMGRMEAIWGSDCREFKPERWIRMVDGRFMSESAYKFTAFNGGPRLCLGKD 506
Query: 705 FAYWIMKCVAASVILRFKMRLASDRPVVTKLSFSLAMKDGLPITLEKR 848
FAY+ MK AS++ + +++ D PVV KL+ ++ MK GL + L KR
Sbjct: 507 FAYYQMKFTVASILFHYHVKVVKDHPVVPKLALTMYMKHGLKVNLVKR 554
>XP_011625110.1 PREDICTED: cytochrome P450 86B1 [Amborella trichopoda]
Length = 523
Score = 237 bits (605), Expect = 1e-70
Identities = 121/291 (41%), Positives = 180/291 (61%), Gaps = 9/291 (3%)
Frame = +3
Query: 3 PTIVWKTIRALRLGTEGRLKVAREEAHGWLDRAMKCRVDVKYEVSDFREGRPMDLLSAMT 182
P VWK ++ +GTE RL+ + H + ++ + D K+E R G +D +T
Sbjct: 233 PPFVWKPMKYFDIGTERRLREVIKVVHKFAEKTVS---DRKFECQ--RSGGILDRSDLLT 287
Query: 183 ----VQSPD---LQEEQHLKDTALSLILAGKDTVTSGLSWFFWAICRHPQVQQKVYEEVS 341
V++ D ++ LKD +S ILAG+DT + L+WFFW + HP ++ ++ +E+
Sbjct: 288 RLIQVRNDDNSPCYSDKFLKDFCISFILAGRDTSSVALAWFFWLLHEHPHIEDRILDEIR 347
Query: 342 AVAKRKPNVEPHETTQ--FSNQELKEMQYLQAALSETLRLYPPIPINIMEAAGDDVLPDG 515
A+ KR+ N+ P F+ ELK+M+YL AALSE+LRLYP +PI+ E DD+LP+G
Sbjct: 348 AIIKRRNNLGPEHLNDVIFTADELKQMEYLHAALSESLRLYPSVPIDFKEVLEDDILPNG 407
Query: 516 TFIKKGTTLIYSVYAHGRNKELWGADAGEFRPERWMSDGKLYTNSEFKFMAFNAGPRRCL 695
T +KKG +IYSV++ R + +WG D EF+PERWM DG + ++FK+ FNAGPR C+
Sbjct: 408 TPVKKGAKIIYSVFSMARIESIWGKDCCEFKPERWMKDGAFVSENQFKYAVFNAGPRLCV 467
Query: 696 GRDFAYWIMKCVAASVILRFKMRLASDRPVVTKLSFSLAMKDGLPITLEKR 848
G+ FAY MK VAAS++LR+ + + PVV KL+ +L MK GL +T R
Sbjct: 468 GKKFAYTQMKMVAASILLRYSVHVVKGHPVVPKLTTTLYMKHGLLVTFHPR 518
>XP_013674320.1 PREDICTED: cytochrome P450 86B1-like [Brassica napus]
Length = 526
Score = 237 bits (604), Expect = 2e-70
Identities = 118/288 (40%), Positives = 185/288 (64%), Gaps = 6/288 (2%)
Frame = +3
Query: 3 PTIVWKTIRALRLGTEGRLKVAREEAHGWLDRAMKCRVDVKYEVSDFREGRPMDLLSAMT 182
P +WK +R L +G E L+ A + HG++++ + R+ + + + R L +
Sbjct: 230 PPFIWKPMRFLDIGYEKGLRKAIDVVHGFVNKMIMDRICMVNN-DETLDNRSDVLTRIIQ 288
Query: 183 VQSPDLQEE------QHLKDTALSLILAGKDTVTSGLSWFFWAICRHPQVQQKVYEEVSA 344
++S E + + S ILAG+DT + +SWFFW I RHPQV+ K+ +E+
Sbjct: 289 IESHKKGNEIGPSTIRFFRQFCTSFILAGRDTSSVAISWFFWVIQRHPQVENKIIQEIRE 348
Query: 345 VAKRKPNVEPHETTQFSNQELKEMQYLQAALSETLRLYPPIPINIMEAAGDDVLPDGTFI 524
+ K++ +P +++ F+ +EL M YLQAA+SETLRLYPPIP+ + +A DD+ PDGTFI
Sbjct: 349 ILKQRG--DPSDSSLFTVKELNNMVYLQAAISETLRLYPPIPMEMKQAIEDDMFPDGTFI 406
Query: 525 KKGTTLIYSVYAHGRNKELWGADAGEFRPERWMSDGKLYTNSEFKFMAFNAGPRRCLGRD 704
+KG+ + +S+YA GR + +WG D EFRPERW+ GK ++ ++K++ FNAGPR CLG+
Sbjct: 407 RKGSRVYFSIYAMGRMESVWGKDCEEFRPERWIQAGKFVSDDQYKYVVFNAGPRMCLGKT 466
Query: 705 FAYWIMKCVAASVILRFKMRLASDRPVVTKLSFSLAMKDGLPITLEKR 848
FAY MK +AASV+LR+ +++A D VV +++ +L MK GL +T+ R
Sbjct: 467 FAYLQMKMIAASVLLRYSIKVAQDHVVVPRVTTNLYMKYGLKVTITPR 514
>XP_013603323.1 PREDICTED: cytochrome P450 86B1-like [Brassica oleracea var.
oleracea]
Length = 526
Score = 237 bits (604), Expect = 2e-70
Identities = 118/295 (40%), Positives = 184/295 (62%), Gaps = 13/295 (4%)
Frame = +3
Query: 3 PTIVWKTIRALRLGTEGRLKVAREEAHGWLDRAMKCRV-------------DVKYEVSDF 143
P +WK ++ L +G E L+ A + HG++++ + R+ DV +
Sbjct: 230 PPFIWKPMKFLDIGYEKGLRKAIDVVHGFVNKMIMDRICMVNDEETLDNRSDVLTRIIQI 289
Query: 144 REGRPMDLLSAMTVQSPDLQEEQHLKDTALSLILAGKDTVTSGLSWFFWAICRHPQVQQK 323
+ + + T++ L+ S ILAG+DT + +SWFFW I RHPQV+ K
Sbjct: 290 DNNKKGNKIGPSTIR--------FLRQFCTSFILAGRDTSSVAISWFFWVIQRHPQVENK 341
Query: 324 VYEEVSAVAKRKPNVEPHETTQFSNQELKEMQYLQAALSETLRLYPPIPINIMEAAGDDV 503
+ +E+ + K++ +P +++ F+ +EL M YLQAA+SETLRLYPPIP+ + +A DD+
Sbjct: 342 IIQEIRQIVKQRG--DPSDSSLFTVKELNNMVYLQAAISETLRLYPPIPMEMKQAIEDDM 399
Query: 504 LPDGTFIKKGTTLIYSVYAHGRNKELWGADAGEFRPERWMSDGKLYTNSEFKFMAFNAGP 683
PDGTFIKKG+ + +S+YA GR + +WG D EFRPERW+ GK ++ ++K++ FNAGP
Sbjct: 400 FPDGTFIKKGSRVYFSIYAMGRMETIWGKDCEEFRPERWIQAGKFVSDDQYKYVVFNAGP 459
Query: 684 RRCLGRDFAYWIMKCVAASVILRFKMRLASDRPVVTKLSFSLAMKDGLPITLEKR 848
R CLG+ FAY MK +AASV+LR+ +++A D VV +++ +L MK GL +T+ R
Sbjct: 460 RLCLGKTFAYLQMKMIAASVLLRYSIKVAQDHVVVPRVTTNLYMKYGLKVTITPR 514