BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ephedra29_contig00012472
         (1842 letters)

Database: ./nr 
           115,041,592 sequences; 42,171,959,267 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

OMO78012.1 tRNA-specific 2-thiouridylase [Corchorus olitorius]        492   e-166
KDO61266.1 hypothetical protein CISIN_1g042717mg, partial [Citru...   488   e-165
OMO81670.1 tRNA-specific 2-thiouridylase [Corchorus capsularis]       484   e-164
XP_012446852.1 PREDICTED: mitochondrial tRNA-specific 2-thiourid...   487   e-164
XP_006470603.1 PREDICTED: tRNA-specific 2-thiouridylase MnmA [Ci...   488   e-164
XP_016565613.1 PREDICTED: tRNA-specific 2-thiouridylase MnmA [Ca...   486   e-164
XP_017983485.1 PREDICTED: tRNA-specific 2-thiouridylase MnmA iso...   486   e-163
XP_002513510.1 PREDICTED: tRNA-specific 2-thiouridylase MnmA [Ri...   486   e-163
GAV59028.1 tRNA_Me_trans domain-containing protein, partial [Cep...   487   e-163
ONK59753.1 tRNA-specific 2-thiouridylase MnmA [Asparagus officin...   484   e-163
XP_017627299.1 PREDICTED: tRNA-specific 2-thiouridylase MnmA [Go...   483   e-162
KVI09388.1 Adenine nucleotide alpha hydrolase-like domain protei...   483   e-162
XP_016750818.1 PREDICTED: tRNA-specific 2-thiouridylase MnmA [Go...   483   e-162
XP_010257320.1 PREDICTED: uncharacterized protein LOC104597469 [...   483   e-162
XP_008801937.1 PREDICTED: tRNA-specific 2-thiouridylase MnmA [Ph...   482   e-162
XP_010906766.1 PREDICTED: uncharacterized protein LOC105033599 [...   481   e-162
KCW87153.1 hypothetical protein EUGRSUZ_B03681 [Eucalyptus grandis]   478   e-162
XP_009407675.1 PREDICTED: uncharacterized protein LOC103990311 i...   481   e-161
XP_019263360.1 PREDICTED: uncharacterized protein LOC109241098 [...   480   e-161
XP_002282827.2 PREDICTED: uncharacterized protein LOC100265527 [...   482   e-161

>OMO78012.1 tRNA-specific 2-thiouridylase [Corchorus olitorius]
          Length = 499

 Score =  492 bits (1267), Expect = e-166
 Identities = 243/442 (54%), Positives = 302/442 (68%), Gaps = 1/442 (0%)
 Frame = +1

Query: 331  DEKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYM 510
            D ++L CC P    R LK+                     H CTAFYLKI     +ENY 
Sbjct: 66   DPRFLSCCMP---HRHLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENYW 122

Query: 511  PGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRI 690
              CPWEDDLKY R+VC+Q  V L+ +  TD YW +VVSY+I EY+ G TPNPD+LCNTRI
Sbjct: 123  TECPWEDDLKYARAVCDQVDVPLEVVHLTDEYWNNVVSYIIEEYRCGRTPNPDVLCNTRI 182

Query: 691  KFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXX 870
            KFG F+D I++M++DYIASGHYA V H      D  +IL LS D +KDQTYF        
Sbjct: 183  KFGAFMDAINSMDYDYIASGHYANVVHPSANQIDKRSILKLSKDMLKDQTYFLSHLSQAQ 242

Query: 871  XXXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILL 1050
                IFPLGC+PK+EVR+LAA   LPNKDRKDSQGICFLGK++FS+FVARHIGEMEGI+L
Sbjct: 243  LKRLIFPLGCLPKDEVRQLAAKFDLPNKDRKDSQGICFLGKIRFSDFVARHIGEMEGIIL 302

Query: 1051 EAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFF 1230
            EAETG FLG HRGFWFYTIGQRQGL L GGPWYVV KD++NNV+FVSRNY+S++ +R  F
Sbjct: 303  EAETGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVFVSRNYFSIDKRRRLF 362

Query: 1231 HVSNIRWIYAT-PEDFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYT 1407
             V +++W+  + P+  E ++CKVRHGPGFYDC  E                +D +++V  
Sbjct: 363  RVGSLKWLSGSPPKHIEQLQCKVRHGPGFYDCSFEIVSG------------KDGSEDVAV 410

Query: 1408 VTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKP 1587
            V L E+DQGLAAGQFAAFY+   C+GSG+I+ S  D  FPVC+KALEI++++DKS LGKP
Sbjct: 411  VHLPEDDQGLAAGQFAAFYEGETCIGSGVILESWDDQGFPVCAKALEISRMEDKSKLGKP 470

Query: 1588 IKIGTPVKSTETKNKFPAERAV 1653
            +KI         K + P E++V
Sbjct: 471  VKI-------MAKPEIPPEQSV 485


>KDO61266.1 hypothetical protein CISIN_1g042717mg, partial [Citrus sinensis]
          Length = 474

 Score =  488 bits (1256), Expect = e-165
 Identities = 245/450 (54%), Positives = 301/450 (66%), Gaps = 3/450 (0%)
 Frame = +1

Query: 331  DEKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYM 510
            + +YL C  P   ++ LK+                     H CTAFYLKI     +EN+ 
Sbjct: 5    EPRYLSCSMPH--QKGLKVAVLLSGGVDSSVALRLLHSAGHSCTAFYLKIWFQEDFENFW 62

Query: 511  PGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRI 690
              CPWE+D+KY ++VC+Q  V L+ +  TD YWK+VVSY+I EY+ G TPNPD+LCNTRI
Sbjct: 63   TECPWEEDMKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRI 122

Query: 691  KFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXX 870
            KFG F+D I +M FDY+ASGHYA+V HS    +   ++L LS D VKDQTYF        
Sbjct: 123  KFGAFMDAISSMEFDYVASGHYAKVVHSSADQEYKPSVLELSKDKVKDQTYFLSHLSQAQ 182

Query: 871  XXXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILL 1050
                IFPLGCI K EVR+ A    LPNKDRKDSQGICFLGK+KFSEFVARHIGEMEG++L
Sbjct: 183  LKRLIFPLGCISKEEVRKFATQFDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGVIL 242

Query: 1051 EAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFF 1230
            EAETG FLG H GFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNYYS + KR  F
Sbjct: 243  EAETGDFLGKHWGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYYSFDKKRRLF 302

Query: 1231 HVSNIRWIYA-TPEDFENIKCKVRHGPGFYDCK--IEHAKDIYWKEHNLPHPVEDSTQEV 1401
             V +++W+    PE+   ++CKVRHGPGFY+C   IEH              VED  + +
Sbjct: 303  RVGSLKWLSGLPPEEISRLQCKVRHGPGFYNCNLMIEH--------------VEDGGENI 348

Query: 1402 YTVTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLG 1581
              V LSE+DQGLAAGQFAAFY+   C+GSG+I+ S  D  FPVC KALEIA+++DKS LG
Sbjct: 349  AVVQLSEDDQGLAAGQFAAFYQGTRCIGSGVILESWDDQGFPVCEKALEIARMEDKSKLG 408

Query: 1582 KPIKIGTPVKSTETKNKFPAERAVKGSKEM 1671
            KPIKI       ET  +F + + V+ S+ +
Sbjct: 409  KPIKIKV---KPETPEEFNSNKGVQSSRNL 435


>OMO81670.1 tRNA-specific 2-thiouridylase [Corchorus capsularis]
          Length = 426

 Score =  484 bits (1247), Expect = e-164
 Identities = 232/383 (60%), Positives = 284/383 (74%), Gaps = 1/383 (0%)
 Frame = +1

Query: 451  HHCTAFYLKIDQDYVYENYMPGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYM 630
            H CTAFYLKI     +ENY   CPWEDDL+Y R+VC+Q  V L+ +  TD YW +VVSY+
Sbjct: 30   HSCTAFYLKIWFQEDFENYWTECPWEDDLQYARAVCDQVDVPLEVVHLTDEYWNNVVSYI 89

Query: 631  ISEYKRGFTPNPDILCNTRIKFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILC 810
            I EY+ G TPNPD+LCNTRIKFG F+D I++M++DYIASGHYA V H      D  +IL 
Sbjct: 90   IEEYRCGRTPNPDVLCNTRIKFGAFMDAINSMDYDYIASGHYANVVHPSADQIDKRSILK 149

Query: 811  LSLDNVKDQTYFXXXXXXXXXXXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLG 990
            LS D +KDQTYF            IFPLGC+PK+EVR+LAA   LPNKDRKDSQGICFLG
Sbjct: 150  LSKDMLKDQTYFLSHLSQAQLKRLIFPLGCLPKDEVRQLAAKCDLPNKDRKDSQGICFLG 209

Query: 991  KVKFSEFVARHIGEMEGILLEAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVE 1170
            K++FS+FVARHIGEMEGI+LEAETG FLG HRGFWFYTIGQRQGL L GGPWYVV KDV+
Sbjct: 210  KIRFSDFVARHIGEMEGIILEAETGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVK 269

Query: 1171 NNVIFVSRNYYSLEMKRSFFHVSNIRWIYAT-PEDFENIKCKVRHGPGFYDCKIEHAKDI 1347
            NNV+FVSRNY+S++ +R  F V +++W+  + P+  E ++CKVRHGPGFYDC  E     
Sbjct: 270  NNVVFVSRNYFSIDKRRRLFRVGSLKWLSGSPPKHIEQLQCKVRHGPGFYDCSFEIVSG- 328

Query: 1348 YWKEHNLPHPVEDSTQEVYTVTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFP 1527
                       +D +++V  V L E+DQGLAAGQFAAFY+   C+GSG+I+ S  D  FP
Sbjct: 329  -----------KDGSEDVAVVHLPEDDQGLAAGQFAAFYEGETCIGSGVILESWDDQGFP 377

Query: 1528 VCSKALEIAKIKDKSVLGKPIKI 1596
            VC+KALEIA+++DKS LGKP+KI
Sbjct: 378  VCAKALEIARMEDKSKLGKPVKI 400


>XP_012446852.1 PREDICTED: mitochondrial tRNA-specific 2-thiouridylase 1 [Gossypium
            raimondii] KJB55374.1 hypothetical protein
            B456_009G073000 [Gossypium raimondii]
          Length = 490

 Score =  487 bits (1253), Expect = e-164
 Identities = 242/427 (56%), Positives = 295/427 (69%), Gaps = 3/427 (0%)
 Frame = +1

Query: 331  DEKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYM 510
            D ++L CC P    + LK+                     H CTAFYLKI     +ENY 
Sbjct: 75   DPRHLSCCMPD---KRLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENYW 131

Query: 511  PGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRI 690
              CPWE+DLKY R+VC+Q  V LQ +  TD YWK+VVSY+I EY+ G TPNPD+LCNTRI
Sbjct: 132  TECPWEEDLKYARAVCDQVDVPLQVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRI 191

Query: 691  KFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXX 870
            KFG F+D I  M++DYIASGHYA V H      + ++IL LS D VKDQTYF        
Sbjct: 192  KFGAFMDAIYNMDYDYIASGHYANVVHPSADQSNKASILELSQDMVKDQTYFLSHLSQFQ 251

Query: 871  XXXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILL 1050
                IFPLGC+ K+EVR+LAA   LPNK RKDSQGICFLGK++FS+FVARHIGEMEGI+L
Sbjct: 252  LKQLIFPLGCLSKDEVRQLAAKFDLPNKGRKDSQGICFLGKIRFSDFVARHIGEMEGIIL 311

Query: 1051 EAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFF 1230
            EAETG FLG HRGFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNY+SL+ +R  F
Sbjct: 312  EAETGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVQKDVKNNVVFVSRNYFSLDKRRRVF 371

Query: 1231 HVSNIRWIYATPED-FENIKCKVRHGPGFYDC--KIEHAKDIYWKEHNLPHPVEDSTQEV 1401
             V +++W   +P +  + ++CKVRHGPGFY+C  K+EH               ED   ++
Sbjct: 372  RVGSLKWFSGSPPNHIDQLQCKVRHGPGFYNCNFKMEHG--------------EDGNDDI 417

Query: 1402 YTVTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLG 1581
              V LSE+DQGLAAGQFAAFY+   C+GSG+I+ S  D  FPVC+KALEIA+++DKS LG
Sbjct: 418  AVVQLSEDDQGLAAGQFAAFYEGQTCIGSGVILESWDDRGFPVCAKALEIARMEDKSELG 477

Query: 1582 KPIKIGT 1602
            KP+KI T
Sbjct: 478  KPVKIMT 484


>XP_006470603.1 PREDICTED: tRNA-specific 2-thiouridylase MnmA [Citrus sinensis]
          Length = 525

 Score =  488 bits (1256), Expect = e-164
 Identities = 245/450 (54%), Positives = 301/450 (66%), Gaps = 3/450 (0%)
 Frame = +1

Query: 331  DEKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYM 510
            + +YL C  P   ++ LK+                     H CTAFYLKI     +EN+ 
Sbjct: 56   EPRYLSCSMPH--QKGLKVAVLLSGGVDSSVALRLLHSAGHSCTAFYLKIWFQEDFENFW 113

Query: 511  PGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRI 690
              CPWE+D+KY ++VC+Q  V L+ +  TD YWK+VVSY+I EY+ G TPNPD+LCNTRI
Sbjct: 114  TECPWEEDMKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRI 173

Query: 691  KFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXX 870
            KFG F+D I +M FDY+ASGHYA+V HS    +   ++L LS D VKDQTYF        
Sbjct: 174  KFGAFMDAISSMEFDYVASGHYAKVVHSSADQEYKPSVLELSKDKVKDQTYFLSHLSQAQ 233

Query: 871  XXXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILL 1050
                IFPLGCI K EVR+ A    LPNKDRKDSQGICFLGK+KFSEFVARHIGEMEG++L
Sbjct: 234  LKRLIFPLGCISKEEVRKFATQFDLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGVIL 293

Query: 1051 EAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFF 1230
            EAETG FLG H GFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNYYS + KR  F
Sbjct: 294  EAETGDFLGKHWGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYYSFDKKRRLF 353

Query: 1231 HVSNIRWIYA-TPEDFENIKCKVRHGPGFYDCK--IEHAKDIYWKEHNLPHPVEDSTQEV 1401
             V +++W+    PE+   ++CKVRHGPGFY+C   IEH              VED  + +
Sbjct: 354  RVGSLKWLSGLPPEEISRLQCKVRHGPGFYNCNLMIEH--------------VEDGGENI 399

Query: 1402 YTVTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLG 1581
              V LSE+DQGLAAGQFAAFY+   C+GSG+I+ S  D  FPVC KALEIA+++DKS LG
Sbjct: 400  AVVQLSEDDQGLAAGQFAAFYQGTRCIGSGVILESWDDQGFPVCEKALEIARMEDKSKLG 459

Query: 1582 KPIKIGTPVKSTETKNKFPAERAVKGSKEM 1671
            KPIKI       ET  +F + + V+ S+ +
Sbjct: 460  KPIKIKV---KPETPEEFNSNKGVQSSRNL 486


>XP_016565613.1 PREDICTED: tRNA-specific 2-thiouridylase MnmA [Capsicum annuum]
          Length = 492

 Score =  486 bits (1252), Expect = e-164
 Identities = 238/423 (56%), Positives = 292/423 (69%), Gaps = 1/423 (0%)
 Frame = +1

Query: 331  DEKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYM 510
            D  YL CC P   K PLK+                     H CTAFYLKI     +EN+ 
Sbjct: 74   DPTYLSCCMPH--KNPLKVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFW 131

Query: 511  PGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRI 690
              CPWEDDL+Y ++VC+Q  + L+ +  TD YWK+VVSY+I EYK G TPNPD+LCNTRI
Sbjct: 132  SECPWEDDLRYAKAVCDQVDIPLEVVHLTDEYWKNVVSYIIEEYKCGRTPNPDVLCNTRI 191

Query: 691  KFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXX 870
            KFG F+D I  M FD++ASGHYAR+ H+ TT  D  +IL LS D VKDQTYF        
Sbjct: 192  KFGAFMDAIGGMEFDFVASGHYARIVHASTTQLDEPSILELSKDMVKDQTYFLSHLSQAQ 251

Query: 871  XXXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILL 1050
                IFPLGCI K+EVR LA    LPN+DRKDSQGICFLGK+KFSEFVARHIGE EG++L
Sbjct: 252  LKRLIFPLGCIQKDEVRMLAKSFNLPNQDRKDSQGICFLGKIKFSEFVARHIGESEGVIL 311

Query: 1051 EAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFF 1230
            EAE+G +LGNHRGFWFYTIGQRQGL L GGPWYVV KD+ NNV++VSRNY+S++ KR  F
Sbjct: 312  EAESGDYLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDIRNNVVYVSRNYFSVDKKRRLF 371

Query: 1231 HVSNIRWIYA-TPEDFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYT 1407
             V +++W+    P     ++CKVRHGPGFY+C             +L   V++  +EV  
Sbjct: 372  RVGSLKWLSGLLPRQISELQCKVRHGPGFYNC-------------SLVVEVDEHGREVAV 418

Query: 1408 VTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKP 1587
            V LSE+DQGLAAGQFAAFY   IC+GSGII+ S  D  +P+C++ALEIA+++DKS LGKP
Sbjct: 419  VRLSEDDQGLAAGQFAAFYDRRICIGSGIILESWDDQGYPICARALEIARMEDKSKLGKP 478

Query: 1588 IKI 1596
            +KI
Sbjct: 479  VKI 481


>XP_017983485.1 PREDICTED: tRNA-specific 2-thiouridylase MnmA isoform X1 [Theobroma
            cacao]
          Length = 501

 Score =  486 bits (1251), Expect = e-163
 Identities = 240/423 (56%), Positives = 294/423 (69%), Gaps = 1/423 (0%)
 Frame = +1

Query: 331  DEKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYM 510
            D ++L CC P   ++ LK+                     H CTAFYLKI     +ENY 
Sbjct: 68   DPRFLSCCMP---EQRLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENYW 124

Query: 511  PGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRI 690
              CPWE+DLK+ RSVC+Q  V L+ +  T  YWK+VVSY+I EYK G TPNPD+LCNTRI
Sbjct: 125  TACPWEEDLKHARSVCDQVYVPLEVVHLTGEYWKNVVSYIIEEYKCGRTPNPDVLCNTRI 184

Query: 691  KFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXX 870
            KFG F+D I++M++DYIASGHYA V H      D  +IL LS D VKDQTYF        
Sbjct: 185  KFGAFMDAINSMDYDYIASGHYANVVHPSADQIDKPSILELSKDMVKDQTYFLSHLSQAQ 244

Query: 871  XXXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILL 1050
                IFPLGC+ K+EVR+LAA   LPNKDRKDSQGICFLGK++FS+FVARHIGEMEGI+L
Sbjct: 245  LKRLIFPLGCLAKDEVRQLAAKFDLPNKDRKDSQGICFLGKIRFSDFVARHIGEMEGIIL 304

Query: 1051 EAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFF 1230
            EAETG FLG HRGFWFYTIGQRQGL L GGPWYVV KDVENNV+FVSRNY+SL+ +R  F
Sbjct: 305  EAETGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVENNVVFVSRNYFSLDKRRRLF 364

Query: 1231 HVSNIRWIYATPED-FENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYT 1407
             V +++W+  +P +  + ++CKVRHGPGFY+C  E     Y             +++V  
Sbjct: 365  RVGSLKWLSGSPPNHIKQLQCKVRHGPGFYNCSFELVSGEY------------GSEDVAV 412

Query: 1408 VTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKP 1587
            V L E+DQGLAAGQFAAFY+   C+GSG+I+ S  D  FPVC+KALEIA+++DKS LGKP
Sbjct: 413  VCLHEDDQGLAAGQFAAFYEGQTCIGSGVILESWDDQGFPVCAKALEIARMEDKSKLGKP 472

Query: 1588 IKI 1596
            +KI
Sbjct: 473  VKI 475


>XP_002513510.1 PREDICTED: tRNA-specific 2-thiouridylase MnmA [Ricinus communis]
            EEF48913.1 tRNA
            (5-methylaminomethyl-2-thiouridylate)-methyltransferase,
            putative [Ricinus communis]
          Length = 497

 Score =  486 bits (1250), Expect = e-163
 Identities = 244/450 (54%), Positives = 300/450 (66%), Gaps = 5/450 (1%)
 Frame = +1

Query: 331  DEKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYM 510
            +  YL CC P   K  L+I                     HHCTAFYLKI     +EN+ 
Sbjct: 57   EPNYLSCCLPD--KNNLRIALLLSGGVDSSVALRLLHAAGHHCTAFYLKIWFQEDFENFW 114

Query: 511  PGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRI 690
              CPWE+DLKY ++VC+Q  + L+ +  TD YWK+VVSY+I EY+ G TPNPD+LCNTRI
Sbjct: 115  SECPWEEDLKYAKAVCDQVDIPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRI 174

Query: 691  KFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXX 870
            KFG F+D I+ M FDY+ASGHYA+V H      +  ++L LS D VKDQTYF        
Sbjct: 175  KFGAFMDAINNMEFDYVASGHYAKVIHPSADEINQPSVLELSKDMVKDQTYFLSHLSQAQ 234

Query: 871  XXXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILL 1050
                IFPLG I K+EVR+LA    LPNKDRKDSQGICFLGK+KFSEFVARHIGEMEGI+L
Sbjct: 235  LRRLIFPLGGISKDEVRKLATLFNLPNKDRKDSQGICFLGKIKFSEFVARHIGEMEGIML 294

Query: 1051 EAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFF 1230
            EAETG FLGNHRGFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNY+S + +R   
Sbjct: 295  EAETGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSFDKRRRLL 354

Query: 1231 HVSNIRWIYAT-PEDFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYT 1407
             V ++RW   + P++   ++CKVRHGPGFY+C +            +  P E   Q+V  
Sbjct: 355  RVGSLRWFNGSPPKETSELQCKVRHGPGFYNCSV------------VIEPGEVDDQDVAI 402

Query: 1408 VTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKP 1587
            V L E+DQGLAAGQFAAFYK   C+GSG+I+ S  D  FPVC +ALE+A+I+DKS LGKP
Sbjct: 403  VQLCEDDQGLAAGQFAAFYKGRTCIGSGVILESWNDQGFPVCERALELARIEDKSELGKP 462

Query: 1588 IKIGT----PVKSTETKNKFPAERAVKGSK 1665
            +KI      PV+  + K+    ER +  S+
Sbjct: 463  VKIKVKPEIPVREFDGKDGIEVERGLNNSQ 492


>GAV59028.1 tRNA_Me_trans domain-containing protein, partial [Cephalotus
            follicularis]
          Length = 558

 Score =  487 bits (1253), Expect = e-163
 Identities = 244/422 (57%), Positives = 292/422 (69%), Gaps = 1/422 (0%)
 Frame = +1

Query: 334  EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYMP 513
            + YL CC     +RPLK+                     H CTAFYLKI     +EN+  
Sbjct: 126  QHYLSCCMLQ--RRPLKVALLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWS 183

Query: 514  GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 693
             CPWE+DLKY R+VC+Q  V L+ +  TD YWK+VVSY+I EY+ G TPNPD+LCNTRIK
Sbjct: 184  ECPWEEDLKYARAVCDQVDVPLEVVHLTDEYWKNVVSYIIQEYRCGRTPNPDVLCNTRIK 243

Query: 694  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXX 873
            FG F+D I  M FDYIASGHYA V H     K+T +IL LS D VKDQTYF         
Sbjct: 244  FGAFMDAISIMEFDYIASGHYANVVHPAANKKNTLSILELSKDMVKDQTYFLSHLSQIQL 303

Query: 874  XXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILLE 1053
               IFPLGCI K+EVR+LA    LPNKDRKDSQGICFLGKVKFSEFV+RHIGEMEGI+LE
Sbjct: 304  KRLIFPLGCISKDEVRKLAMKFDLPNKDRKDSQGICFLGKVKFSEFVSRHIGEMEGIILE 363

Query: 1054 AETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFH 1233
            AETG FLG HRGFWFYTIGQRQGL L GGPWYVV KDV+NN +FVSRNY+S + +R  F 
Sbjct: 364  AETGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNAVFVSRNYFSFDKRRRLFR 423

Query: 1234 VSNIRWIYAT-PEDFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTV 1410
            V +++W+  + PE    ++CKVRHGPGFY C ++      W+        ED +++V  V
Sbjct: 424  VGSLKWLGGSPPEQISELQCKVRHGPGFYYCSLK------WELG------EDGSKDVVVV 471

Query: 1411 TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 1590
             LSE+DQGLAAGQFAAFY+ + C+GSG+I+ S  D  FPVC+KALEIAK+ D S LGKP+
Sbjct: 472  QLSEDDQGLAAGQFAAFYQGSTCIGSGVILDSWDDQGFPVCAKALEIAKMVDISKLGKPV 531

Query: 1591 KI 1596
            KI
Sbjct: 532  KI 533


>ONK59753.1 tRNA-specific 2-thiouridylase MnmA [Asparagus officinalis]
          Length = 485

 Score =  484 bits (1245), Expect = e-163
 Identities = 238/421 (56%), Positives = 289/421 (68%)
 Frame = +1

Query: 334  EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYMP 513
            + YL+C  PGG    LK+                     H CTAFYLKI     +EN+  
Sbjct: 70   DPYLRCSMPGG---RLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWS 126

Query: 514  GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 693
             CPW+DDL+Y R+VCE+  V L+    TD YW +VVS++ISEY+ G TPNPD+LCNTRIK
Sbjct: 127  ECPWDDDLRYARAVCEKVDVPLEVAHLTDEYWNNVVSHIISEYRSGRTPNPDVLCNTRIK 186

Query: 694  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXX 873
            FG FL+ +++M FDY+ASGHYARV HS     D  +IL LS D VKDQTYF         
Sbjct: 187  FGAFLEAVESMKFDYVASGHYARVTHSSLDGNDMPSILSLSKDMVKDQTYFLSHLSQKQL 246

Query: 874  XXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILLE 1053
               + PLGCI K+EVR LA  M LPNKDRKDSQGICFLGKVKFSEFVA+HIGE EGI+LE
Sbjct: 247  KRLLLPLGCISKDEVRRLATLMDLPNKDRKDSQGICFLGKVKFSEFVAKHIGEQEGIILE 306

Query: 1054 AETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFH 1233
            AETG +LG+HRGFWFYT+GQRQGL L GGPWYVV KDV+NNV+FVSRNYYSL+ KR  F 
Sbjct: 307  AETGDYLGDHRGFWFYTVGQRQGLRLPGGPWYVVEKDVQNNVVFVSRNYYSLDKKRRKFR 366

Query: 1234 VSNIRWIYATPEDFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTVT 1413
            V  ++WI  +P     ++CKVRHGP F++C I    D            +    EV TV 
Sbjct: 367  VGTLKWISGSPPKKSQLRCKVRHGPSFHNCSITMESD------------DTGYGEVVTVD 414

Query: 1414 LSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPIK 1593
            LSE+DQGLAAGQFAAFY  + C+GSG+I+ S  D  FP+C+KALEIA+++DKS LGKP++
Sbjct: 415  LSEDDQGLAAGQFAAFYDGDSCIGSGVILDSWDDKSFPICAKALEIAQLQDKSKLGKPVR 474

Query: 1594 I 1596
            I
Sbjct: 475  I 475


>XP_017627299.1 PREDICTED: tRNA-specific 2-thiouridylase MnmA [Gossypium arboreum]
            KHG00493.1 tRNA-specific 2-thiouridylase mnmA [Gossypium
            arboreum]
          Length = 490

 Score =  483 bits (1243), Expect = e-162
 Identities = 240/427 (56%), Positives = 293/427 (68%), Gaps = 3/427 (0%)
 Frame = +1

Query: 331  DEKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYM 510
            D ++L CC P    + LK+                     H CTAFYLKI     +ENY 
Sbjct: 75   DPRHLSCCMPD---KRLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENYW 131

Query: 511  PGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRI 690
              CPWE+DLKY R+VC+Q  V LQ +  TD YW +VVSY+I EY+ G TPNPD+LCNTRI
Sbjct: 132  TECPWEEDLKYARAVCDQVDVPLQVVQLTDEYWNNVVSYIIEEYRCGRTPNPDVLCNTRI 191

Query: 691  KFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXX 870
            KFG F+D I  M++DYIASGHYA V H      + ++IL LS D VKDQTYF        
Sbjct: 192  KFGAFMDAIYNMDYDYIASGHYANVVHPSADQSNKASILELSQDMVKDQTYFLSHLSQFQ 251

Query: 871  XXXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILL 1050
                IFPLGC+ K+EVR+LAA   LPNK RKDSQGICFLGK++FS+FVARHIGEMEGI+L
Sbjct: 252  LKQLIFPLGCLSKDEVRQLAAKFDLPNKGRKDSQGICFLGKIRFSDFVARHIGEMEGIIL 311

Query: 1051 EAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFF 1230
            EAETG FLG HRGFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNY+SL+ +R  F
Sbjct: 312  EAETGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVQKDVKNNVVFVSRNYFSLDKRRRVF 371

Query: 1231 HVSNIRWIYATPED-FENIKCKVRHGPGFYDC--KIEHAKDIYWKEHNLPHPVEDSTQEV 1401
             V +++W   +P +  + ++CKVRHGPGFY+C  K+EH               ED   ++
Sbjct: 372  RVGSLKWFSGSPPNHIDQLQCKVRHGPGFYNCNFKMEHG--------------EDGNDDI 417

Query: 1402 YTVTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLG 1581
              V L E+DQGLAAGQFAAFY+   C+GSG+I+ S  D  FPVC+KALEIA+++DKS LG
Sbjct: 418  AVVQLYEDDQGLAAGQFAAFYEGQTCIGSGVILESWDDRGFPVCAKALEIARMEDKSELG 477

Query: 1582 KPIKIGT 1602
            KP+KI T
Sbjct: 478  KPVKIMT 484


>KVI09388.1 Adenine nucleotide alpha hydrolase-like domain protein [Cynara
            cardunculus var. scolymus]
          Length = 509

 Score =  483 bits (1244), Expect = e-162
 Identities = 240/440 (54%), Positives = 293/440 (66%), Gaps = 1/440 (0%)
 Frame = +1

Query: 331  DEKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYM 510
            D  YL C  P    RPLK+                     H CTAFYLKI     +EN+ 
Sbjct: 81   DRDYLSCSMPNS-NRPLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFW 139

Query: 511  PGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRI 690
              CPWE+DLKY ++VC Q  V L+ +  TD YW  VVSY+I EYK G TPNPD+LCNTRI
Sbjct: 140  SECPWEEDLKYAKAVCNQVDVPLEVVHLTDEYWDKVVSYLIDEYKCGRTPNPDVLCNTRI 199

Query: 691  KFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXX 870
            KFG F+D I  M+FD++ASGHYA+V H+ +   +  + L LS D VKDQTYF        
Sbjct: 200  KFGAFMDAISNMDFDFVASGHYAKVVHADSDKTNGISFLQLSKDMVKDQTYFLSYLSQAQ 259

Query: 871  XXXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILL 1050
                + PLGCIPKN+VR+LA    LPN+DRKDSQGICFLGK+KFSEFV RHIGE EGI+L
Sbjct: 260  LKRLVLPLGCIPKNDVRKLARKFDLPNQDRKDSQGICFLGKLKFSEFVGRHIGEREGIIL 319

Query: 1051 EAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFF 1230
            EAETG FLG HRGFWFYTIGQRQGLGL GGPWYVV KDV+NNV+FVSRNYYS++ +R  F
Sbjct: 320  EAETGDFLGAHRGFWFYTIGQRQGLGLAGGPWYVVEKDVKNNVVFVSRNYYSVDKRRRSF 379

Query: 1231 HVSNIRWIYAT-PEDFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYT 1407
            HV + RWI  + P++   ++CKVRHGP FYDC +                 E+ ++ + T
Sbjct: 380  HVGSFRWISGSPPQNLNQLRCKVRHGPDFYDCTLH------------MEVAENRSESIGT 427

Query: 1408 VTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKP 1587
            V L ++DQGLAAGQFAAFY++ +C+GSG+I+ S  D  FPVC KA EIAK++DKS LGKP
Sbjct: 428  VQLPKDDQGLAAGQFAAFYEEQVCVGSGVILESWDDKGFPVCDKAREIAKMEDKSKLGKP 487

Query: 1588 IKIGTPVKSTETKNKFPAER 1647
            +KI   +   E   K P  R
Sbjct: 488  VKI---MPKPEDVRKIPDRR 504


>XP_016750818.1 PREDICTED: tRNA-specific 2-thiouridylase MnmA [Gossypium hirsutum]
          Length = 490

 Score =  483 bits (1242), Expect = e-162
 Identities = 240/427 (56%), Positives = 293/427 (68%), Gaps = 3/427 (0%)
 Frame = +1

Query: 331  DEKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYM 510
            D ++L CC P    + LK+                     H CTAFYLKI     +ENY 
Sbjct: 75   DPRHLSCCMPD---KRLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENYW 131

Query: 511  PGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRI 690
              CPWE+DLKY R+VC+Q  V LQ +  TD YW +VVSY+I EY+ G TPNPD+LCNTRI
Sbjct: 132  TECPWEEDLKYARAVCDQVDVPLQVVHLTDEYWNNVVSYIIEEYRCGRTPNPDVLCNTRI 191

Query: 691  KFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXX 870
            KFG F+D I  M++DYIASGHYA V H      + ++IL LS D VKDQTYF        
Sbjct: 192  KFGAFMDAIYNMDYDYIASGHYANVVHPSADQSNKASILELSQDMVKDQTYFLSHLSQFQ 251

Query: 871  XXXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILL 1050
                IFPLGC+ K+EVR+LAA   LPNK RKDSQGICFLGK++FS+FVARHIGEMEGI+L
Sbjct: 252  LKQLIFPLGCLSKDEVRQLAAKFDLPNKGRKDSQGICFLGKIRFSDFVARHIGEMEGIIL 311

Query: 1051 EAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFF 1230
            EAETG FLG HRGFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNY+SL+ +R  F
Sbjct: 312  EAETGDFLGKHRGFWFYTIGQRQGLRLPGGPWYVVQKDVKNNVVFVSRNYFSLDKRRRVF 371

Query: 1231 HVSNIRWIYATPED-FENIKCKVRHGPGFYDC--KIEHAKDIYWKEHNLPHPVEDSTQEV 1401
             V +++W   +P +  + ++CKVRHGPGFY+C  K+EH               ED   ++
Sbjct: 372  RVGSLKWFSGSPPNHIDQLQCKVRHGPGFYNCNFKMEHG--------------EDGNDDI 417

Query: 1402 YTVTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLG 1581
              V L E+DQGLAAGQFAAFY+   C+GSG+I+ S  D  FPVC+KALEIA+++DKS LG
Sbjct: 418  AVVQLYEDDQGLAAGQFAAFYEGQTCIGSGVILESWDDRGFPVCAKALEIARMEDKSELG 477

Query: 1582 KPIKIGT 1602
            KP+KI T
Sbjct: 478  KPVKIMT 484


>XP_010257320.1 PREDICTED: uncharacterized protein LOC104597469 [Nelumbo nucifera]
          Length = 531

 Score =  483 bits (1244), Expect = e-162
 Identities = 244/438 (55%), Positives = 295/438 (67%), Gaps = 5/438 (1%)
 Frame = +1

Query: 334  EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYMP 513
            E YL C  PG   + LK+                     H CTAFYLKI     +EN+  
Sbjct: 67   EPYLSCSMPG---KRLKVAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWS 123

Query: 514  GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 693
             CPWE+DL+YV++VC+Q  V L+ +  TD YW +VVSY+I EY+ G TPNPD+LCNTRIK
Sbjct: 124  ACPWEEDLEYVKAVCDQVEVPLEVVHLTDEYWDNVVSYIIQEYRYGRTPNPDVLCNTRIK 183

Query: 694  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXX 873
            FG FLD I  M FDY+ASGHYA V H  T  KD  +IL LS D VKDQTYF         
Sbjct: 184  FGAFLDAISNMEFDYVASGHYANVIHPCTDQKDGPSILELSEDKVKDQTYFLSHLSQAQL 243

Query: 874  XXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILLE 1053
               +FPLGCI KNEVR+LAA + LPN+DRKDSQGICFLGK+KFSEF+A+HIGEMEGILLE
Sbjct: 244  KRLLFPLGCISKNEVRKLAAKLELPNRDRKDSQGICFLGKIKFSEFIAKHIGEMEGILLE 303

Query: 1054 AETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFH 1233
            AETG FLG H GFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNYYS + +R  F 
Sbjct: 304  AETGDFLGKHHGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYYSFDKRRRRFR 363

Query: 1234 VSNIRWIYATPED-FENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTV 1410
            V +++W+   P D F  ++CKVRHGPGFYDC +            +    ED  +    V
Sbjct: 364  VGSLKWLAGLPPDQFNKLQCKVRHGPGFYDCSL------------IMETGEDGDRTSAVV 411

Query: 1411 TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 1590
             LSE+DQGLAAGQFAAFY+   C+GSG+I+       FPVC+KAL+IA+++DKS LGKP+
Sbjct: 412  HLSEDDQGLAAGQFAAFYQGKTCIGSGVILECWDSQGFPVCAKALQIARMEDKSKLGKPV 471

Query: 1591 KI----GTPVKSTETKNK 1632
            KI       +KS+E  +K
Sbjct: 472  KIKVNPENSLKSSEITDK 489


>XP_008801937.1 PREDICTED: tRNA-specific 2-thiouridylase MnmA [Phoenix dactylifera]
          Length = 500

 Score =  482 bits (1240), Expect = e-162
 Identities = 240/422 (56%), Positives = 295/422 (69%), Gaps = 1/422 (0%)
 Frame = +1

Query: 334  EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYMP 513
            E +L C  PG   + LK+                     H CTAFYLKI     ++N+  
Sbjct: 88   EPFLGCSMPG---KRLKVAVLLSGGVDSSVALRLLHAAGHDCTAFYLKIWFQEDFKNFWS 144

Query: 514  GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 693
             CPW++DLKY R+VC+Q  + L+ +  +D YWK+VVS++ISEY+ G TPNPD+LCNTRIK
Sbjct: 145  ECPWDEDLKYARTVCDQVDLPLEVVHLSDEYWKNVVSHIISEYRSGRTPNPDVLCNTRIK 204

Query: 694  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXX 873
            FG FL+ I  M FDY+ASGHYA V HS +  +D  +IL LS D VKDQTYF         
Sbjct: 205  FGAFLEAIGGMEFDYVASGHYAHVIHSSSEQRDGPSILKLSKDMVKDQTYFLSHLSQFQL 264

Query: 874  XXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILLE 1053
               +FP+GCIPK+EVR LA+ M LPNKDRKDSQGICFLGKVKF EFVA+ IGE+EGILLE
Sbjct: 265  RRLLFPIGCIPKDEVRRLASLMDLPNKDRKDSQGICFLGKVKFCEFVAKQIGEVEGILLE 324

Query: 1054 AETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFH 1233
            AETG +LG HRGFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNY+SL+ +R  F 
Sbjct: 325  AETGDYLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVQNNVVFVSRNYFSLDKRRRTFR 384

Query: 1234 VSNIRWIYAT-PEDFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTV 1410
            V ++ W   + P + E ++CKVRHGPGFYDC I       W         +D  +EV  V
Sbjct: 385  VGSLNWFSGSPPSENEQLQCKVRHGPGFYDCSITE-----WSS-------KDGHEEVVEV 432

Query: 1411 TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 1590
             +SE+DQGLAAGQFAAFY ++ICLGSG+I+ S  D  FP+C+KALEIAK++DKS LGKP+
Sbjct: 433  HISEDDQGLAAGQFAAFYCNDICLGSGVILDSWDDKSFPICTKALEIAKMEDKSKLGKPV 492

Query: 1591 KI 1596
            KI
Sbjct: 493  KI 494


>XP_010906766.1 PREDICTED: uncharacterized protein LOC105033599 [Elaeis guineensis]
          Length = 495

 Score =  481 bits (1239), Expect = e-162
 Identities = 253/472 (53%), Positives = 313/472 (66%), Gaps = 4/472 (0%)
 Frame = +1

Query: 193  ASIKVSNARRLSATERRGIGVSSEQGITEETTC---LFPQHEQKQWRPADEKYLQCCWPG 363
            +S+++ +  R ++T       SS   ++    C   LFP           E YL C  PG
Sbjct: 35   SSLRLLSISRPTSTRPLTTSSSSSPALSSPPPCPPPLFPSISSNAVHL--EPYLGCSMPG 92

Query: 364  GVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYMPGCPWEDDLKY 543
               R LK+                     H CTAFYLKI     +EN+   CPW++DLKY
Sbjct: 93   ---RHLKVAVLLSGGVDSSVALRLLHAAGHDCTAFYLKIWFQEDFENFWSECPWDEDLKY 149

Query: 544  VRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIKFGEFLDYIDT 723
             R+VC+Q  V L+ +  +D YWK+VVS++ISEY+ G TPNPD+LCNTRIKFG FL+ I +
Sbjct: 150  ARAVCDQVDVPLEVVHLSDEYWKNVVSHIISEYRSGRTPNPDVLCNTRIKFGAFLEAIGS 209

Query: 724  MNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXXXXXIFPLGCI 903
            M FDY+ASGHYA V HS +  +D  +IL LS D VKDQTYF            +FPLGCI
Sbjct: 210  MEFDYVASGHYAYVIHSSSEQRDGPSILKLSKDMVKDQTYFLSHLSQFQLRRLLFPLGCI 269

Query: 904  PKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILLEAETGAFLGNH 1083
            PK+EVR LA+ M LPNKDRKDSQGICFLGKVKFSEFVA+HIGEMEGILLEAETG +LG H
Sbjct: 270  PKDEVRWLASLMDLPNKDRKDSQGICFLGKVKFSEFVAKHIGEMEGILLEAETGDYLGKH 329

Query: 1084 RGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFHVSNIRWIYAT 1263
            +GFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNY+SL+ +R  F V ++ W   +
Sbjct: 330  QGFWFYTIGQRQGLRLPGGPWYVVEKDVQNNVVFVSRNYFSLDKRRRTFCVGSLNWFSGS 389

Query: 1264 -PEDFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTVTLSENDQGLA 1440
             P + E ++CKVRHGPGFYDC I       W   N         ++V  V +SE+DQGLA
Sbjct: 390  PPSNNELLQCKVRHGPGFYDCSIAE-----WSSKN-------GQEDVIEVHISEDDQGLA 437

Query: 1441 AGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPIKI 1596
            AGQFAAFY ++ICLGSG+I+ S  D  F + +KALEIAK++DKS LGKP+KI
Sbjct: 438  AGQFAAFYCNDICLGSGVILDSWDDKSFCISTKALEIAKMEDKSKLGKPVKI 489


>KCW87153.1 hypothetical protein EUGRSUZ_B03681 [Eucalyptus grandis]
          Length = 408

 Score =  478 bits (1230), Expect = e-162
 Identities = 234/390 (60%), Positives = 285/390 (73%), Gaps = 1/390 (0%)
 Frame = +1

Query: 451  HHCTAFYLKIDQDYVYENYMPGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYM 630
            H CTAFYLKI     +EN+   CPWE+DLKY ++VCEQ  V L+ +  TD YWK+VVSY+
Sbjct: 30   HSCTAFYLKIWFQEDFENFWSECPWEEDLKYAKAVCEQVDVPLEVVHLTDEYWKNVVSYI 89

Query: 631  ISEYKRGFTPNPDILCNTRIKFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILC 810
            I EY+ G TPNPD+LCNTRIKFG F+D I++M +D+IASGHYA+V HS +   D ++ L 
Sbjct: 90   IEEYRSGRTPNPDVLCNTRIKFGAFMDAINSMEYDFIASGHYAKVVHS-SNHIDNASELK 148

Query: 811  LSLDNVKDQTYFXXXXXXXXXXXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLG 990
            LS D VKDQTYF            IFPLGCI KNEVR+LAA   LPN+DRKDSQGICFLG
Sbjct: 149  LSEDMVKDQTYFLSHLSQAQLKRLIFPLGCISKNEVRKLAARFDLPNQDRKDSQGICFLG 208

Query: 991  KVKFSEFVARHIGEMEGILLEAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVE 1170
            K+KFSEFVARHIGEMEGI+LEAETG FLGNHRGFWFYTIGQRQGL L GGPWYVV KD +
Sbjct: 209  KIKFSEFVARHIGEMEGIILEAETGDFLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDTK 268

Query: 1171 NNVIFVSRNYYSLEMKRSFFHVSNIRW-IYATPEDFENIKCKVRHGPGFYDCKIEHAKDI 1347
            NNV+FVSRNY+S++ +R  F V +++W I A P +   ++CKVRHGPGFY+C +      
Sbjct: 269  NNVVFVSRNYFSVDKRRRLFRVGSLKWLIGAPPTEISQLQCKVRHGPGFYNCSLSLELG- 327

Query: 1348 YWKEHNLPHPVEDSTQEVYTVTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFP 1527
                       E+  ++V  V L E+DQGLAAGQF AFY+D  C+GSG+I+ S  D  FP
Sbjct: 328  -----------ENGQEDVAVVQLPEDDQGLAAGQFTAFYQDRTCIGSGVILESWNDQGFP 376

Query: 1528 VCSKALEIAKIKDKSVLGKPIKIGTPVKST 1617
            VC KALEIA+++DKS LGKP+KI    + T
Sbjct: 377  VCQKALEIARMEDKSKLGKPVKIKVKPEGT 406


>XP_009407675.1 PREDICTED: uncharacterized protein LOC103990311 isoform X1 [Musa
            acuminata subsp. malaccensis] XP_018682618.1 PREDICTED:
            uncharacterized protein LOC103990311 isoform X1 [Musa
            acuminata subsp. malaccensis] XP_018682619.1 PREDICTED:
            uncharacterized protein LOC103990311 isoform X1 [Musa
            acuminata subsp. malaccensis]
          Length = 506

 Score =  481 bits (1238), Expect = e-161
 Identities = 242/425 (56%), Positives = 295/425 (69%), Gaps = 1/425 (0%)
 Frame = +1

Query: 334  EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYMP 513
            E YL C  PG   R L++                     H CTAFYLKI     ++N+  
Sbjct: 96   EPYLGCSMPG---RRLRVAVLLSGGVDSSTALRLLHAAGHDCTAFYLKIWFQEDFQNFWS 152

Query: 514  GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 693
             CPW++DLKY R+VC+Q  V L+ +  +D YW +VV ++ISEY+ G TPNPD+LCNTRIK
Sbjct: 153  ECPWDEDLKYARAVCDQVDVPLEVVHLSDEYWNNVVCHIISEYRSGRTPNPDVLCNTRIK 212

Query: 694  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXX 873
            FG FL+ I +M FDY+ASGHYA V HS  +S+D  +IL LS D +KDQTYF         
Sbjct: 213  FGAFLEAIASMEFDYVASGHYAHVVHS--SSEDGPSILKLSKDLIKDQTYFLSHLSQSQL 270

Query: 874  XXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILLE 1053
               +FPLGC+ K+EVR +A  M LPNKDRKDSQGICFLGKVKFSEFVA+HIGE+EGILLE
Sbjct: 271  KRLLFPLGCLRKDEVRRIATLMDLPNKDRKDSQGICFLGKVKFSEFVAKHIGELEGILLE 330

Query: 1054 AETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFH 1233
            AETG +LGNH GFWFYTIGQRQGL L+GGPWYVV KDV NNV+FVSRNY+SL+ +R  FH
Sbjct: 331  AETGDYLGNHHGFWFYTIGQRQGLRLSGGPWYVVEKDVRNNVVFVSRNYFSLDKRRRTFH 390

Query: 1234 VSNIRWIY-ATPEDFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTV 1410
            V ++ W   A PE+ E ++CKVRHGPGFYDC I     I   E NL             V
Sbjct: 391  VGSLNWFSGAPPENTEQLQCKVRHGPGFYDCSIMAVPGIDGDEDNL------------VV 438

Query: 1411 TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 1590
             LSE+DQGLAAGQFAAFY  ++C+GSG+I+ S  +  FP+CSKALEIA+++DKS LGKPI
Sbjct: 439  HLSEDDQGLAAGQFAAFYHGDVCIGSGVIMDSWDEKCFPICSKALEIARMEDKSKLGKPI 498

Query: 1591 KIGTP 1605
            +I  P
Sbjct: 499  RIMNP 503


>XP_019263360.1 PREDICTED: uncharacterized protein LOC109241098 [Nicotiana attenuata]
            OIT37212.1 hypothetical protein A4A49_08038 [Nicotiana
            attenuata]
          Length = 488

 Score =  480 bits (1236), Expect = e-161
 Identities = 234/423 (55%), Positives = 289/423 (68%), Gaps = 1/423 (0%)
 Frame = +1

Query: 331  DEKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYM 510
            D  YL C  P   K PLK+                     H CTAFYLKI     +EN+ 
Sbjct: 70   DPTYLSCSMPH--KNPLKVAVLVSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFW 127

Query: 511  PGCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRI 690
              CPWEDDLKY ++VC+Q  V L+ +  TD YW +VVSY+I EYK G TPNPD+LCNTRI
Sbjct: 128  SECPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWNNVVSYIIEEYKCGRTPNPDVLCNTRI 187

Query: 691  KFGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXX 870
            KFG F+D I  M FD++ASGHYA++ H+ T   D  +IL LS D VKDQTYF        
Sbjct: 188  KFGAFMDAISGMEFDFVASGHYAKIVHASTEHLDEPSILELSKDMVKDQTYFLSHLSQAQ 247

Query: 871  XXXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILL 1050
                IFPLGCIPK+EVR LA    LPN+DRKDSQGICFLGK+KFSEFVARHIGE EG++L
Sbjct: 248  LKRLIFPLGCIPKDEVRMLAKSFNLPNQDRKDSQGICFLGKIKFSEFVARHIGESEGVIL 307

Query: 1051 EAETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFF 1230
            EAE+G +LGNHRGFWFYTIGQRQGL L GGPWYVV KD++NNV++VSRNY+S++ KR  F
Sbjct: 308  EAESGDYLGNHRGFWFYTIGQRQGLRLPGGPWYVVEKDIKNNVVYVSRNYFSVDKKRRLF 367

Query: 1231 HVSNIRWIYA-TPEDFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYT 1407
             V +++W+    P     ++CKVRHGPGFY+C             +L   V++  QEV  
Sbjct: 368  RVGSLKWLSGLPPRQISQLQCKVRHGPGFYNC-------------SLAMEVDEHGQEVAV 414

Query: 1408 VTLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKP 1587
            V +SE+DQGLAAGQFAAFY    C+GSG+I+ S  D  +P+C +A+EIA+++DKS LGKP
Sbjct: 415  VRISEDDQGLAAGQFAAFYDGRTCIGSGVILESWDDQGYPICERAIEIARMEDKSRLGKP 474

Query: 1588 IKI 1596
            +KI
Sbjct: 475  VKI 477


>XP_002282827.2 PREDICTED: uncharacterized protein LOC100265527 [Vitis vinifera]
            CBI16383.3 unnamed protein product, partial [Vitis
            vinifera]
          Length = 544

 Score =  482 bits (1241), Expect = e-161
 Identities = 245/449 (54%), Positives = 296/449 (65%), Gaps = 5/449 (1%)
 Frame = +1

Query: 334  EKYLQCCWPGGVKRPLKIXXXXXXXXXXXXXXXXXXXXXHHCTAFYLKIDQDYVYENYMP 513
            + YL C  P      LKI                     H CTAFYLKI     +EN+  
Sbjct: 76   QPYLSCSLPNN---RLKIAVLLSGGVDSSVALRLLHAAGHSCTAFYLKIWFQEDFENFWS 132

Query: 514  GCPWEDDLKYVRSVCEQAGVDLQRIDFTDRYWKSVVSYMISEYKRGFTPNPDILCNTRIK 693
             CPWEDDLKY ++VC+Q  V L+ +  TD YWK+VVSY+I EY+ G TPNPD+LCNTRIK
Sbjct: 133  ECPWEDDLKYAKAVCDQVDVPLEVVHLTDEYWKNVVSYIIEEYRCGRTPNPDVLCNTRIK 192

Query: 694  FGEFLDYIDTMNFDYIASGHYARVQHSGTTSKDTSAILCLSLDNVKDQTYFXXXXXXXXX 873
            FG F+D I +  FDY+ASGHYA V HS T   D  ++L LS D VKDQTYF         
Sbjct: 193  FGAFMDAISSKEFDYVASGHYANVVHSFTDQMDKPSVLELSKDMVKDQTYFLSHLSQIQL 252

Query: 874  XXXIFPLGCIPKNEVRELAADMGLPNKDRKDSQGICFLGKVKFSEFVARHIGEMEGILLE 1053
               IFPLGCIPK+EVR LA  + LPN+DRKDSQGICFLGK+KFSEFVARHIGE EGI+LE
Sbjct: 253  KRLIFPLGCIPKDEVRSLATKLDLPNRDRKDSQGICFLGKIKFSEFVARHIGEKEGIILE 312

Query: 1054 AETGAFLGNHRGFWFYTIGQRQGLGLTGGPWYVVAKDVENNVIFVSRNYYSLEMKRSFFH 1233
            AETG  LG HRGFWFYTIGQRQGL L GGPWYVV KDV+NNV+FVSRNY+SL+ +R  F 
Sbjct: 313  AETGDLLGKHRGFWFYTIGQRQGLRLPGGPWYVVEKDVKNNVVFVSRNYFSLDKRRRLFR 372

Query: 1234 VSNIRWIYATPE-DFENIKCKVRHGPGFYDCKIEHAKDIYWKEHNLPHPVEDSTQEVYTV 1410
            V +++WI   P      ++CKVRHGPGFY+C                 P ED  ++V  V
Sbjct: 373  VGSLKWISREPPGQINQLQCKVRHGPGFYNCSF------------TMEPGEDGHEDVAVV 420

Query: 1411 TLSENDQGLAAGQFAAFYKDNICLGSGIIVGSSKDAYFPVCSKALEIAKIKDKSVLGKPI 1590
             LSE+DQGLAAGQF AFY+   C+GSG+I+ S  D  FPVC+KALEIA+++DKS LGKP+
Sbjct: 421  QLSEDDQGLAAGQFTAFYQGRSCIGSGVILESWDDQGFPVCAKALEIARMEDKSKLGKPV 480

Query: 1591 KI----GTPVKSTETKNKFPAERAVKGSK 1665
            KI     TP K ++  +    +R +  S+
Sbjct: 481  KIKVRPETPPKGSDQIDGIKLDRELANSQ 509