BLASTX 2.2.26 [Sep-21-2011]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Ephedra29_contig00011929
         (1822 letters)

Database: ./nr 
           115,041,592 sequences; 42,171,959,267 total letters

Searching..................................................done



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

XP_006845255.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein ...   365   e-117
GAV75205.1 DUF2392 domain-containing protein, partial [Cephalotu...   348   e-110
XP_015898174.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein ...   347   e-109
XP_002278289.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein ...   344   e-109
XP_010246740.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein ...   342   e-108
XP_015898173.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein ...   340   e-107
XP_010906284.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein ...   337   e-106
XP_013449604.1 cytoplasmic tRNA 2-thiolation protein [Medicago t...   337   e-106
XP_006489985.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein ...   335   e-105
XP_006421388.1 hypothetical protein CICLE_v10004945mg [Citrus cl...   335   e-105
XP_007204337.1 hypothetical protein PRUPE_ppa005400mg [Prunus pe...   335   e-105
XP_018841765.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein ...   334   e-105
XP_019154594.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein ...   335   e-105
XP_007162194.1 hypothetical protein PHAVU_001G132100g [Phaseolus...   334   e-105
XP_002529606.2 PREDICTED: cytoplasmic tRNA 2-thiolation protein ...   332   e-105
XP_014494738.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein ...   333   e-105
XP_019444040.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein ...   333   e-105
XP_016543722.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein ...   333   e-104
JAT44977.1 Cytoplasmic tRNA 2-thiolation protein 2, partial [Ant...   333   e-104
XP_004249262.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein ...   333   e-104

>XP_006845255.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Amborella
            trichopoda] ERN06930.1 hypothetical protein
            AMTR_s00005p00261900 [Amborella trichopoda]
          Length = 458

 Score =  365 bits (938), Expect = e-117
 Identities = 207/447 (46%), Positives = 289/447 (64%), Gaps = 8/447 (1%)
 Frame = -3

Query: 1604 VAMPRSCSKCSENKAQIRATNQTEFLCRDCLHANLLSKFKTSVNLNAMILPSDKVLLAFS 1425
            +A  + C KC E     +A NQTE LC  CL ++L +KFK +V  +AMI P+DK+L+AFS
Sbjct: 34   IANTKLCCKCQERDGVAKA-NQTESLCPPCLRSSLFAKFKLAVTSHAMISPTDKLLVAFS 92

Query: 1424 GGPSSRVVLEFVRELRSKAQRDADASRDSILQVFDVGVAFVDESTVAHGAQT--CQLIEE 1251
            GGP+SRV LEFV E++SKAQR+ADAS++  L VF VGVAF+DE  V+  + T  C+ +EE
Sbjct: 93   GGPASRVALEFVNEMQSKAQRNADASKEKCLAVFQVGVAFIDEKMVSQASSTEACRAVEE 152

Query: 1250 IKLLLSGNVQPKEKIHVAALECLWSENEESC--KQTLLDLLASIHDTTSKEDLLGYLRMQ 1077
            ++ ++S    P +++HV  +E + S + E    K  L DLL +I D T KED + YLRM 
Sbjct: 153  VRDIVSSMGNPSKELHVFPIEDICSIDTEGSDGKDRLKDLLDAIDDVTGKEDFVQYLRMI 212

Query: 1076 VLQKIAIEHGYSKLLLGLNASRIAVHVLSSTVKGQGYSLPADVRYIDARWEVPVVLPLRD 897
             LQKIA++HGYSKL+LGL  SRIA HV+++TVKGQGYSLPADV+Y+DARWEVPV+ PL D
Sbjct: 213  SLQKIAVDHGYSKLVLGLCTSRIACHVIAATVKGQGYSLPADVQYVDARWEVPVLCPLHD 272

Query: 896  CASKELTMLCHFNSWKSINISSTSILGAPNSINALVGAFVSSMQDENPSREQTIVRTAEK 717
            C  +ELTMLC  ++ K++      +     S+N+++ +FV+ +Q+ENPSRE+TIV+TAEK
Sbjct: 273  CVIQELTMLCQLDNLKTLQ----PLKVHQPSVNSIISSFVALLQEENPSRERTIVKTAEK 328

Query: 716  LKAFTFNKLPENTAVSHLS-RRRQEVLKNNTRSEASIELLCPVCSSPLNERDFDFKTSPQ 540
            L++F+FNKL    ++  L+ RRR  +L+ N     S E LC +CSSPL +       S  
Sbjct: 329  LRSFSFNKLVH--SIDKLAFRRRHRLLRFNCIESTSSEALCAICSSPLEKSGVPCSNS-- 384

Query: 539  SIENFKELYISLSRGGKSECSPSSLFMCMCCPTCRFQILDDQ--KESNIHLLLPNLMRER 366
                  EL  S S           +F  +CCP+C+FQIL ++       +  LP  M  +
Sbjct: 385  ------ELEYSQSE--------MVMFRSVCCPSCQFQILPEEPLDMERFYSFLPQSMIRK 430

Query: 365  AHENAFDRDSWIRKNIEDCLLS-DEDG 288
            A+ +     + +R  I+DCLLS DEDG
Sbjct: 431  ANGDVIADQNSLRDQIQDCLLSEDEDG 457


>GAV75205.1 DUF2392 domain-containing protein, partial [Cephalotus follicularis]
          Length = 525

 Score =  348 bits (894), Expect = e-110
 Identities = 196/466 (42%), Positives = 281/466 (60%), Gaps = 21/466 (4%)
 Frame = -3

Query: 1619 NNTVVVAMPRS-CSKCSENKAQIRATNQTE-----FLCRDCLHANLLSKFKTSVNLNAMI 1458
            NNT      ++ C KC  N+     +   +       C DC   NL  KF+ +V  +AMI
Sbjct: 80   NNTKTTKTNQNLCIKCKTNQPTCGGSGSDDGDGGRLFCADCFRTNLYGKFRLAVTSHAMI 139

Query: 1457 LPSDKVLLAFSGGPSSRVVLEFVRELRSKAQRDADASRDSILQVFDVGVAFVDESTV--A 1284
             P D VL+AFSGGPSSRV L+F+ E++ +AQ++ DASRD  L VF VGVAF+ ES++   
Sbjct: 140  TPDDNVLVAFSGGPSSRVALQFIHEMQYRAQKNFDASRDRSLPVFRVGVAFISESSINPI 199

Query: 1283 HGAQTCQLIEEIKLLLSGNVQPKEKIHVAALECLWSENEESCKQTLLDLLASIHDTTSKE 1104
               +  + IEE++ ++S    P ++ HV  +E ++S +    + +L +LLA + DTT KE
Sbjct: 200  LSDKEDRAIEEMRSIVSNLAPPPKQFHVVPIESIYSSDSSDARDSLKELLAGVSDTTGKE 259

Query: 1103 DLLGYLRMQVLQKIAIEHGYSKLLLGLNASRIAVHVLSSTVK---------GQGYSLPAD 951
            DL+ +LRM  LQKIA E+GY++L LG   SRIA HVLS+TVK         GQGYSLPAD
Sbjct: 260  DLMLHLRMLSLQKIASENGYNRLALGSCTSRIACHVLSATVKLSCLYSLQQGQGYSLPAD 319

Query: 950  VRYIDARWEVPVVLPLRDCASKELTMLCHFNSWKSINISSTSILGAPNSINALVGAFVSS 771
            ++Y+DARWE+P+VLPLRDC ++EL+ LCH N  K++ + +    G  + IN L+ +FV+ 
Sbjct: 320  IQYVDARWEIPIVLPLRDCLAQELSTLCHLNGLKTVEVRN----GPCSGINGLISSFVTL 375

Query: 770  MQDENPSREQTIVRTAEKLKAFTFNKLPE--NTAVSHLSRRRQEVLKNNTRSEASIELLC 597
            +Q+ENPSRE TIVRTA KL  F FN++PE     VS  +R+RQ+          S E  C
Sbjct: 376  LQEENPSRECTIVRTAGKLTPFHFNRIPEIDGCNVSLAARKRQKKFNLKANEPISSETFC 435

Query: 596  PVCSSPLNERDFDFKTSPQSIENFKELYISLSRGGKSECSPSSLFMCMCCPTCRFQILDD 417
            P+C+SPLN+ D       QS++       S+   G +           CC +CRFQIL  
Sbjct: 436  PICNSPLNQSDL------QSMKGLPNSQTSIDAFGAT-----------CCSSCRFQILPK 478

Query: 416  QKES--NIHLLLPNLMRERAHENAFDRDSWIRKNIEDCLLSDEDGD 285
            +  S  +++ LLP+ +  RA +  +   S +R+ I+DCLLSD +G+
Sbjct: 479  EPTSMESLYALLPHPLVARAKQGIYGNMSSLREQIQDCLLSDSEGE 524


>XP_015898174.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 isoform X2
            [Ziziphus jujuba]
          Length = 505

 Score =  347 bits (890), Expect = e-109
 Identities = 198/444 (44%), Positives = 271/444 (61%), Gaps = 10/444 (2%)
 Frame = -3

Query: 1586 CSKCSENKAQI---RATNQTEFLCRDCLHANLLSKFKTSVNLNAMILPSDKVLLAFSGGP 1416
            C KC  N+      R T+     C DC  +NL  KF+ +V  NAMI P+D VL+AFSGG 
Sbjct: 83   CIKCKANQPMSFAGRRTDDDARFCGDCFRSNLFGKFRLAVTSNAMICPTDNVLVAFSGGA 142

Query: 1415 SSRVVLEFVRELRSKAQRDADASRDSILQVFDVGVAFVDESTV--AHGAQTCQLIEEIKL 1242
            SSRV L+FV E++ KAQ++ DASRD  L VF VGVAF+DES++      +  + IE+I+L
Sbjct: 143  SSRVALQFVHEMQHKAQKNFDASRDRSLPVFGVGVAFIDESSIFTVPEDEVTKGIEDIRL 202

Query: 1241 LLSGNVQPKEKIHVAALECLWSENEESCKQTLLDLLASIHDTTSKEDLLGYLRMQVLQKI 1062
            ++S    P +++HV  +E L+S +       L  LL ++ D T KEDLL YLRM  LQKI
Sbjct: 203  IVSSLTPPTKELHVVPIESLYSSDSNDGSDRLKKLLDAVCDATGKEDLLVYLRMLSLQKI 262

Query: 1061 AIEHGYSKLLLGLNASRIAVHVLSSTVKGQGYSLPADVRYIDARWEVPVVLPLRDCASKE 882
            A E+GY++L+LG   SRIA HVL++TVKG GYSLPAD++Y+D+RW VPVVLPLRDC  +E
Sbjct: 263  ASENGYNRLVLGSCTSRIACHVLTATVKGHGYSLPADIQYVDSRWGVPVVLPLRDCLIQE 322

Query: 881  LTMLCHFNSWKSINISSTSILGAPNSINALVGAFVSSMQDENPSREQTIVRTAEKLKAFT 702
            L MLCH +  K++ ++        +SIN LV +FV+ +Q+ENPSRE TIVRTA KL  F 
Sbjct: 323  LNMLCHLDGLKTVELNK----NPSSSINGLVSSFVTLLQEENPSRECTIVRTAGKLTPFH 378

Query: 701  FNKLPE--NTAVSHLSRRRQEVLKNNTRSEASIELLCPVCSSPLNERDFDFKTSPQSIEN 528
            FN++PE  ++ V   ++RRQ+          S E  CP+C+SPL++ D            
Sbjct: 379  FNRIPEINDSNVPLATQRRQKRYNLKPTESISSESFCPICNSPLSKSDL----------- 427

Query: 527  FKELYISLSRGGKSECSPSS-LFMCMCCPTCRFQIL--DDQKESNIHLLLPNLMRERAHE 357
                   LS      C  +S LF   CC +CRFQIL  D     + + LLP  +  RA  
Sbjct: 428  -------LSFKNVESCDTNSVLFSAACCSSCRFQILPKDPSSVGDFYSLLPQPVVARAKC 480

Query: 356  NAFDRDSWIRKNIEDCLLSDEDGD 285
             + D  S +R+ I+DC+LSD + +
Sbjct: 481  GSADNYSLLREQIKDCILSDSEDE 504


>XP_002278289.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Vitis vinifera]
            CBI29838.3 unnamed protein product, partial [Vitis
            vinifera]
          Length = 468

 Score =  344 bits (883), Expect = e-109
 Identities = 204/446 (45%), Positives = 275/446 (61%), Gaps = 13/446 (2%)
 Frame = -3

Query: 1586 CSKCSENKA-----QIRATNQTEFLCRDCLHANLLSKFKTSVNLNAMILPSDKVLLAFSG 1422
            C KC  N+       +         C DC   NL  KFK +V  NAMI P+D VL+AFSG
Sbjct: 47   CLKCKVNETLASSGTVSVDGDIGRFCFDCFRGNLFGKFKLAVTSNAMISPTDNVLVAFSG 106

Query: 1421 GPSSRVVLEFVRELRSKAQRDADASRDSILQVFDVGVAFVDESTVAH--GAQTCQLIEEI 1248
            GPSSRV L+FV E++ KAQ++ DASRD  L VF VGVAFVDES V+     +  + I+++
Sbjct: 107  GPSSRVALQFVHEMQHKAQKNFDASRDRSLPVFGVGVAFVDESAVSRIPSDEFDKAIQDM 166

Query: 1247 KLLLSGNVQPKEKIHVAALECLWSENEESCKQTLLDLLASIHDTTSKEDLLGYLRMQVLQ 1068
            K ++S    P +  HV  +E ++S N    +  L +LL ++ D T KEDLL +LRM  LQ
Sbjct: 167  KSIVSNLAPPDKAFHVVPIESVYSSNG---RDRLEELLNAVKDVTGKEDLLQHLRMLSLQ 223

Query: 1067 KIAIEHGYSKLLLGLNASRIAVHVLSSTVKGQGYSLPADVRYIDARWEVPVVLPLRDCAS 888
            K A E+GY+KLLLG   SRIA HV+S+TVKGQGYSLPAD++Y+DARWE+PVVLPLRDC +
Sbjct: 224  KNAYENGYTKLLLGSCTSRIACHVISATVKGQGYSLPADIQYVDARWEIPVVLPLRDCLA 283

Query: 887  KELTMLCHFNSWKSINISSTSILGAPNS-INALVGAFVSSMQDENPSREQTIVRTAEKLK 711
            +EL MLC  +  K++      +L +P+S IN LV +FV+ +Q+ENPSRE TIVRTA KL 
Sbjct: 284  QELNMLCRLDGLKTM-----EVLNSPHSGINGLVASFVTLLQEENPSRECTIVRTAGKLT 338

Query: 710  AFTFNKLPE--NTAVSHLSRRRQEVLKNNTRSEASIELLCPVCSSPLNERDFDFKTSPQS 537
             F FN++PE  ++ V   SRRRQ+            E  CP+C+SPL++ D         
Sbjct: 339  PFHFNQIPESDDSNVHLASRRRQKKFNLKPNESVPPESYCPICNSPLSKSD--------- 389

Query: 536  IENFKELYISLSRGGKSECSPSSLFMCMCCPTCRFQILDDQKESNIHL--LLPNLMRERA 363
                   +I+LS  G S+ + + +F   CCP+C+FQIL  +  S  H   LLP     +A
Sbjct: 390  -------HINLSSLG-SDQTNADIFGATCCPSCQFQILPKEPVSMEHFYSLLPQQFVVQA 441

Query: 362  HENAFDRDSWIRKNIEDCLLSD-EDG 288
             + +F     +R+ I+D LLSD EDG
Sbjct: 442  KDRSFSMQRQLREQIQDFLLSDSEDG 467


>XP_010246740.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 isoform X1
            [Nelumbo nucifera]
          Length = 470

 Score =  342 bits (876), Expect = e-108
 Identities = 200/439 (45%), Positives = 279/439 (63%), Gaps = 7/439 (1%)
 Frame = -3

Query: 1586 CSKCSENKAQIRATNQTEFLCRDCLHANLLSKFKTSVNLNAMILPSDKVLLAFSGGPSSR 1407
            C KC  ++A    T     LC  C  ++L  KFK SV  + MI P D +L+AFSGGPSSR
Sbjct: 53   CFKCKVDEA---ITGDEAHLCLGCFRSSLFGKFKFSVTSHDMINPFDNILVAFSGGPSSR 109

Query: 1406 VVLEFVRELRSKAQRDADASRDSILQVFDVGVAFVDESTVAH--GAQTCQLIEEIKLLLS 1233
            VVL+FV E++ KAQR+ DASRD  L VF VGVAF+DE+ ++     +  + I++I+L++S
Sbjct: 110  VVLQFVHEMQQKAQRNFDASRDRSLPVFGVGVAFIDETVISSIPSHEYEKAIQDIRLIVS 169

Query: 1232 GNVQPKEKIHVAALECLWSENEESCKQTLLDLLASIHDTTSKEDLLGYLRMQVLQKIAIE 1053
                P++K+H+  +E ++S + +     L +LL +I D T KEDL+ +L M  LQKIA++
Sbjct: 170  SLAPPEKKLHIVPIENIYSLDSKGETSRLKELLHAIDDDTGKEDLVHHLHMMSLQKIALD 229

Query: 1052 HGYSKLLLGLNASRIAVHVLSSTVKGQGYSLPADVRYIDARWEVPVVLPLRDCASKELTM 873
            +GY+KL+LG  ASRIA HV+S+TVKGQGYSLPAD++Y+DARWE+PVVLPLRDC  +EL M
Sbjct: 230  NGYNKLVLGSCASRIACHVISATVKGQGYSLPADIQYVDARWEIPVVLPLRDCLIQELNM 289

Query: 872  LCHFNSWKSINISSTSILGAPNSINALVGAFVSSMQDENPSREQTIVRTAEKLKAFTFNK 693
            +C  +S K +      + G+ + IN LV +FV+ +Q ENPSRE+TIVRTAEKL  F FN+
Sbjct: 290  ICSHDSLKIVK----QLDGSGSGINVLVSSFVTLLQKENPSRERTIVRTAEKLTPFHFNR 345

Query: 692  LPE-NTAVSHLSRRRQEVLKNNTRSEASI--ELLCPVCSSPLNERDFDFKTSPQSIENFK 522
            LPE +   +H+S RR+   K N +S  SI  EL CP+C+SPLN+ D     S +   N +
Sbjct: 346  LPETDDPNNHVSSRRRH-KKFNPKSNESIPSELFCPICNSPLNKVD-----SERLKSNLR 399

Query: 521  ELYISLSRGGKSECSPSSLFMCMCCPTCRFQILDDQKESNIHL--LLPNLMRERAHENAF 348
                S    G S           CC +C+FQIL  +  S  H   LLP  M +RA  +  
Sbjct: 400  HCETSADILGIS-----------CCSSCQFQILPKKSSSKEHFYSLLPQPMIDRAKYHIS 448

Query: 347  DRDSWIRKNIEDCLLSDED 291
                 +R+ I+D LLS+++
Sbjct: 449  RDQDLLREQIKDFLLSEDE 467


>XP_015898173.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 isoform X1
            [Ziziphus jujuba]
          Length = 512

 Score =  340 bits (872), Expect = e-107
 Identities = 198/451 (43%), Positives = 271/451 (60%), Gaps = 17/451 (3%)
 Frame = -3

Query: 1586 CSKCSENKAQI---RATNQTEFLCRDCLHANLLSKFKTSVNLNAMILPSDKVLLAFSGGP 1416
            C KC  N+      R T+     C DC  +NL  KF+ +V  NAMI P+D VL+AFSGG 
Sbjct: 83   CIKCKANQPMSFAGRRTDDDARFCGDCFRSNLFGKFRLAVTSNAMICPTDNVLVAFSGGA 142

Query: 1415 SSRVVLEFVRELRSKAQRDADASRDSILQVFDVGVAFVDESTV--AHGAQTCQLIEEIKL 1242
            SSRV L+FV E++ KAQ++ DASRD  L VF VGVAF+DES++      +  + IE+I+L
Sbjct: 143  SSRVALQFVHEMQHKAQKNFDASRDRSLPVFGVGVAFIDESSIFTVPEDEVTKGIEDIRL 202

Query: 1241 LLSGNVQPKEKIHVAALECLWSENEESCKQTLLDLLASIHDTTSKEDLLGYLRMQVLQKI 1062
            ++S    P +++HV  +E L+S +       L  LL ++ D T KEDLL YLRM  LQKI
Sbjct: 203  IVSSLTPPTKELHVVPIESLYSSDSNDGSDRLKKLLDAVCDATGKEDLLVYLRMLSLQKI 262

Query: 1061 AIEHGYSKLLLGLNASRIAVHVLSSTVK-------GQGYSLPADVRYIDARWEVPVVLPL 903
            A E+GY++L+LG   SRIA HVL++TVK       G GYSLPAD++Y+D+RW VPVVLPL
Sbjct: 263  ASENGYNRLVLGSCTSRIACHVLTATVKVRTFFQQGHGYSLPADIQYVDSRWGVPVVLPL 322

Query: 902  RDCASKELTMLCHFNSWKSINISSTSILGAPNSINALVGAFVSSMQDENPSREQTIVRTA 723
            RDC  +EL MLCH +  K++ ++        +SIN LV +FV+ +Q+ENPSRE TIVRTA
Sbjct: 323  RDCLIQELNMLCHLDGLKTVELNK----NPSSSINGLVSSFVTLLQEENPSRECTIVRTA 378

Query: 722  EKLKAFTFNKLPE--NTAVSHLSRRRQEVLKNNTRSEASIELLCPVCSSPLNERDFDFKT 549
             KL  F FN++PE  ++ V   ++RRQ+          S E  CP+C+SPL++ D     
Sbjct: 379  GKLTPFHFNRIPEINDSNVPLATQRRQKRYNLKPTESISSESFCPICNSPLSKSDL---- 434

Query: 548  SPQSIENFKELYISLSRGGKSECSPSS-LFMCMCCPTCRFQIL--DDQKESNIHLLLPNL 378
                          LS      C  +S LF   CC +CRFQIL  D     + + LLP  
Sbjct: 435  --------------LSFKNVESCDTNSVLFSAACCSSCRFQILPKDPSSVGDFYSLLPQP 480

Query: 377  MRERAHENAFDRDSWIRKNIEDCLLSDEDGD 285
            +  RA   + D  S +R+ I+DC+LSD + +
Sbjct: 481  VVARAKCGSADNYSLLREQIKDCILSDSEDE 511


>XP_010906284.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Elaeis
            guineensis]
          Length = 468

 Score =  337 bits (865), Expect = e-106
 Identities = 200/440 (45%), Positives = 270/440 (61%), Gaps = 6/440 (1%)
 Frame = -3

Query: 1586 CSKCSENKAQIRATNQTEFLCRDCLHANLLSKFKTSVNLNAMILPSDKVLLAFSGGPSSR 1407
            CSKC E             LC  C   ++ +KFK +V  NAMI P+D VL+AFSGG +SR
Sbjct: 53   CSKCGEEGRYYNG------LCAACFRISVYNKFKLAVTSNAMISPTDNVLVAFSGGAASR 106

Query: 1406 VVLEFVRELRSKAQRDADASRDSILQVFDVGVAFVDESTVAHGA--QTCQLIEEIKLLLS 1233
            V L+FV E++ K+ +  DAS+   L VF VGVAF+DES  +     +T + IEE++  +S
Sbjct: 107  VALQFVHEMQGKSLKSWDASKSQALPVFGVGVAFIDESIFSLNLSDETEKAIEELRSAVS 166

Query: 1232 GNVQPKEKIHVAALECLWSENEESCKQTLLDLLASIHDTTSKEDLLGYLRMQVLQKIAIE 1053
                P +++H+  ++ + S + E+ +  L +LL  I D T K+D L YLRM  LQKIA++
Sbjct: 167  NLTPPHKELHIIPIQNICSLSSENGRSRLNELLEMISDATGKDDFLHYLRMLSLQKIALD 226

Query: 1052 HGYSKLLLGLNASRIAVHVLSSTVKGQGYSLPADVRYIDARWEVPVVLPLRDCASKELTM 873
             GYSK+LLG   S IA H+LS+T KGQGYSLPADV+Y+DAR EVPVVLPLRDC ++EL+M
Sbjct: 227  KGYSKILLGSCTSTIARHILSATAKGQGYSLPADVQYVDARLEVPVVLPLRDCLAQELSM 286

Query: 872  LCHFNSWKSINISSTSILGAPNSINALVGAFVSSMQDENPSREQTIVRTAEKLKAFTFNK 693
            LCH + +K+  +      G    IN+LV +FV+ +Q+ENPSRE+TIVRTAEKL+ F FNK
Sbjct: 287  LCHLDGFKTQQLLERPCTG----INSLVSSFVARLQEENPSRERTIVRTAEKLRPFGFNK 342

Query: 692  LPENTAVSHLSRRRQEVLKNNTRSEASI-ELLCPVCSSPLNERDFDFKTSPQSIENFKEL 516
              EN     L  R +   +N    E+S+ ++LCP+C SPLNE +     S QS       
Sbjct: 343  FIENGYHDFLPSRLRCKFQNVKNGESSLSDVLCPICGSPLNESELQCLESIQS------- 395

Query: 515  YISLSRGGKSECSPSSLFMCMCCPTCRFQILDDQKESNIHL--LLPNLMRERAHEN-AFD 345
                    K+E     +F   CC +C FQIL   K S  H   +LP LM ER  ++  FD
Sbjct: 396  --------KAE-KKVEIFAGHCCTSCSFQILPKGKASLEHFYSVLPQLMTERMKDSLCFD 446

Query: 344  RDSWIRKNIEDCLLSDEDGD 285
            R S +R  IEDCLL+D+D D
Sbjct: 447  R-SQLRDQIEDCLLADDDDD 465


>XP_013449604.1 cytoplasmic tRNA 2-thiolation protein [Medicago truncatula]
            KEH23632.1 cytoplasmic tRNA 2-thiolation protein
            [Medicago truncatula]
          Length = 458

 Score =  337 bits (863), Expect = e-106
 Identities = 185/422 (43%), Positives = 263/422 (62%), Gaps = 8/422 (1%)
 Frame = -3

Query: 1526 CRDCLHANLLSKFKTSVNLNAMILPSDKVLLAFSGGPSSRVVLEFVRELRSKAQRDADAS 1347
            C DC  +NL  KF+ +V  NAMI P+DKVL+AFSGGPSSRV L+FV +L+ +A ++ +AS
Sbjct: 60   CADCFRSNLFGKFRLAVTSNAMISPTDKVLVAFSGGPSSRVALQFVHDLQERAHKNFEAS 119

Query: 1346 RDSILQVFDVGVAFVDESTV----AHGAQTCQLIEEIKLLLSGNVQPKEKIHVAALECLW 1179
            RD  L VF VGV F+DES V    AH  +  + +E I L++S    P++++H   +E ++
Sbjct: 120  RDRSLPVFGVGVVFIDESAVLPIPAHEME--EAVEVISLIVSSLAPPRKELHFVPIETVY 177

Query: 1178 SENEESCKQTLLDLLASIHDTTSKEDLLGYLRMQVLQKIAIEHGYSKLLLGLNASRIAVH 999
            S +    K  L++L+  + DTT +ED+L  LRM  LQK+A E GY+++LLG   SRIA H
Sbjct: 178  SSDSSDGKDRLIELMNVVSDTTGREDMLLSLRMLALQKVASEFGYNRILLGSCISRIAAH 237

Query: 998  VLSSTVKGQGYSLPADVRYIDARWEVPVVLPLRDCASKELTMLCHFNSWKSINISSTSIL 819
            VLS+TVKGQGYSLPAD++Y+DARWE+P VLPLRDC ++E+ M C+ +S K+I +S++   
Sbjct: 238  VLSATVKGQGYSLPADIQYVDARWEIPAVLPLRDCFAQEINMFCNLDSLKTIKLSTS--- 294

Query: 818  GAPNSINALVGAFVSSMQDENPSREQTIVRTAEKLKAFTFNKLPE--NTAVSHLSRRRQE 645
               +SIN+LV +FV+ +Q+ENPSRE TIVRTA KL  F FN++PE  +  V   +RRRQ+
Sbjct: 295  -PSSSINSLVSSFVALLQEENPSRESTIVRTAGKLIPFQFNRIPEIVDGNVPLATRRRQK 353

Query: 644  VLKNNTRSEASIELLCPVCSSPLNERDFDFKTSPQSIENFKELYISLSRGGKSECSPSSL 465
                 T    S E  CP+C+SPL + +                   +     + C  S  
Sbjct: 354  RYNLKTNESVSSESFCPLCNSPLAKSEI------------------VDWSNHNNCKSSDT 395

Query: 464  FMCMCCPTCRFQIL--DDQKESNIHLLLPNLMRERAHENAFDRDSWIRKNIEDCLLSDED 291
            F   CC +C+FQIL  D       +  LP+ M  RA+       + +R+ I+DCLLSD +
Sbjct: 396  FYTSCCSSCQFQILPPDTTSMEKFYTDLPHSMVARANRVNNGNLNALREQIQDCLLSDGE 455

Query: 290  GD 285
             +
Sbjct: 456  NE 457


>XP_006489985.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Citrus sinensis]
          Length = 455

 Score =  335 bits (860), Expect = e-105
 Identities = 193/440 (43%), Positives = 264/440 (60%), Gaps = 6/440 (1%)
 Frame = -3

Query: 1586 CSKCSENKAQIRATNQTEFLCRDCLHANLLSKFKTSVNLNAMILPSDKVLLAFSGGPSSR 1407
            C KC  N+    A    +  C DC  +NL  KF+ +V  NA+I P+D VL+AFSGGP SR
Sbjct: 38   CVKCKANEPTAGAGEDGKH-CLDCFRSNLFGKFRLAVASNALITPADNVLVAFSGGPCSR 96

Query: 1406 VVLEFVRELRSKAQRDADASRDSILQVFDVGVAFVDESTV--AHGAQTCQLIEEIKLLLS 1233
            V L+FV EL+ +AQ++ DAS+D  L VF VGV FVDE+       ++    I+EIKL++S
Sbjct: 97   VALQFVHELQQRAQKNFDASKDRSLPVFGVGVVFVDETAYYPVPSSEIDNAIQEIKLIVS 156

Query: 1232 GNVQPKEKIHVAALECLWSENEESCKQTLLDLLASIHDTTSKEDLLGYLRMQVLQKIAIE 1053
                P +++HV  +E ++  N+   ++    L+ S+ D T KEDLL  LRM  LQK A E
Sbjct: 157  NLSPPTKELHVIPIESIFCSNQCDRRERFKKLVDSVSDATGKEDLLLQLRMLSLQKFASE 216

Query: 1052 HGYSKLLLGLNASRIAVHVLSSTVKGQGYSLPADVRYIDARWEVPVVLPLRDCASKELTM 873
            +G+++LLLGL  SRIA HV+++TVKGQGYSLPAD++Y DARWE+PVVLPLRDC ++EL M
Sbjct: 217  NGFNRLLLGLCTSRIACHVITATVKGQGYSLPADIQYADARWEIPVVLPLRDCLAQELNM 276

Query: 872  LCHFNSWKSINISSTSILGAPNSINALVGAFVSSMQDENPSREQTIVRTAEKLKAFTFNK 693
            LC  +  K++ + + +  G    IN LV +FV  +Q+ENPSRE TI+RTA KL  F FNK
Sbjct: 277  LCQLDCLKTVELLNQTHSG----INGLVSSFVKILQEENPSRESTIMRTAGKLTPFHFNK 332

Query: 692  LPE--NTAVSHLSRRRQEVLKNNTRSEASIELLCPVCSSPLNERDFDFKTSPQSIENFKE 519
            +PE  +++V   S+RRQ+          S E  C +C SPLN+ D    TS  S +N   
Sbjct: 333  IPELNDSSVPLASQRRQKRFNLKPNESISSESFCSLCYSPLNQSDL---TSLSSHDN--- 386

Query: 518  LYISLSRGGKSECSPSSLFMCMCCPTCRFQIL--DDQKESNIHLLLPNLMRERAHENAFD 345
                        C  S +F+  CC +CRFQI   D       + LLP  +  RA      
Sbjct: 387  ------------CKNSDIFVAACCSSCRFQIFPNDPSSMEKFYSLLPEPLVARAKHVRNG 434

Query: 344  RDSWIRKNIEDCLLSDEDGD 285
              S +R+ I+D LLSD + D
Sbjct: 435  DSSLLREQIQDFLLSDSEDD 454


>XP_006421388.1 hypothetical protein CICLE_v10004945mg [Citrus clementina] ESR34628.1
            hypothetical protein CICLE_v10004945mg [Citrus
            clementina]
          Length = 455

 Score =  335 bits (860), Expect = e-105
 Identities = 194/440 (44%), Positives = 264/440 (60%), Gaps = 6/440 (1%)
 Frame = -3

Query: 1586 CSKCSENKAQIRATNQTEFLCRDCLHANLLSKFKTSVNLNAMILPSDKVLLAFSGGPSSR 1407
            C KC  N+    A    +  C DC  +NL  KF+ +V  NA+I P+D VL+AFSGGPSSR
Sbjct: 38   CVKCKANEPTPGAGEDGKH-CLDCFRSNLFGKFRLAVASNALITPADNVLVAFSGGPSSR 96

Query: 1406 VVLEFVRELRSKAQRDADASRDSILQVFDVGVAFVDESTV--AHGAQTCQLIEEIKLLLS 1233
            V L+FV EL+ +AQ++ DAS+D  L VF VGV FVDE+       ++    I+EIKL++S
Sbjct: 97   VALQFVHELQQRAQKNFDASKDRSLPVFGVGVVFVDETAYYPVPSSEIDNAIQEIKLIVS 156

Query: 1232 GNVQPKEKIHVAALECLWSENEESCKQTLLDLLASIHDTTSKEDLLGYLRMQVLQKIAIE 1053
                P +++HV  +E ++  N    ++    L+ S+ D T KEDLL  LRM  LQK A E
Sbjct: 157  NLSPPTKELHVIPIESIFCSNPCDGRERFKKLVDSVSDATGKEDLLLQLRMLSLQKFASE 216

Query: 1052 HGYSKLLLGLNASRIAVHVLSSTVKGQGYSLPADVRYIDARWEVPVVLPLRDCASKELTM 873
            +GY++LLLGL  SRIA HV+++TVKG+GYSLPAD++Y DARWE+PVVLPLRDC ++EL M
Sbjct: 217  NGYNRLLLGLCTSRIACHVITATVKGRGYSLPADIQYADARWEIPVVLPLRDCLAQELNM 276

Query: 872  LCHFNSWKSINISSTSILGAPNSINALVGAFVSSMQDENPSREQTIVRTAEKLKAFTFNK 693
            LC  +  K++ + + +  G    IN LV +FV  +Q+ENPSRE TI+RTA KL  F FNK
Sbjct: 277  LCQLDCLKTVELLNQTHSG----INGLVSSFVKILQEENPSRESTIMRTAGKLTPFHFNK 332

Query: 692  LPE--NTAVSHLSRRRQEVLKNNTRSEASIELLCPVCSSPLNERDFDFKTSPQSIENFKE 519
            +PE  +++V   S+RRQ+          S E  C +C SPLN+ D    TS  S +N   
Sbjct: 333  IPELNDSSVPLASQRRQKRFNLKPNESISSESFCSLCYSPLNQSDL---TSLSSHDN--- 386

Query: 518  LYISLSRGGKSECSPSSLFMCMCCPTCRFQIL--DDQKESNIHLLLPNLMRERAHENAFD 345
                        C  S +F+  CC +CRFQI   D       + LLP  +  RA      
Sbjct: 387  ------------CKNSDIFVAACCSSCRFQIFPKDPSSMEKFYSLLPEPLVARAKHVRNG 434

Query: 344  RDSWIRKNIEDCLLSDEDGD 285
              S +R+ I+D LLSD + D
Sbjct: 435  DSSLLREQIQDFLLSDSEDD 454


>XP_007204337.1 hypothetical protein PRUPE_ppa005400mg [Prunus persica] ONH95274.1
            hypothetical protein PRUPE_7G060900 [Prunus persica]
          Length = 463

 Score =  335 bits (860), Expect = e-105
 Identities = 193/443 (43%), Positives = 274/443 (61%), Gaps = 9/443 (2%)
 Frame = -3

Query: 1586 CSKCSENK---AQIRATNQTEFLCRDCLHANLLSKFKTSVNLNAMILPSDKVLLAFSGGP 1416
            C KC  N+   +     +   F C DC   NL  KF+ +V  NAMI P+D VL+AFSGGP
Sbjct: 47   CLKCKANQPISSNSDGGDDARF-CIDCFRGNLFGKFRFAVTSNAMIAPTDNVLVAFSGGP 105

Query: 1415 SSRVVLEFVRELRSKAQRDADASRDSILQVFDVGVAFVDESTV--AHGAQTCQLIEEIKL 1242
            SSRV L+FV E+ SKAQ++ DA RD  L VF VGVAFVDES+V  A   +T ++I +++L
Sbjct: 106  SSRVALQFVHEMHSKAQKNFDACRDRSLPVFGVGVAFVDESSVYSAPSNETDKVIGDVRL 165

Query: 1241 LLSGNVQPKEKIHVAALECLWSENEESCKQTLLDLLASIHDTTSKEDLLGYLRMQVLQKI 1062
            ++     P +++++  +E ++S +    ++ L  LL ++ D T +EDLL +LRM  LQK+
Sbjct: 166  IVENLAPPSKELYIVPIESVYSSDSGDGRERLKKLLDAVGDATGREDLLLHLRMLALQKV 225

Query: 1061 AIEHGYSKLLLGLNASRIAVHVLSSTVKGQGYSLPADVRYIDARWEVPVVLPLRDCASKE 882
            A ++GY++LLLG   SRIA HV+S+TVKGQGYSLPADV+Y+DARWE+PVVLPLRDC +KE
Sbjct: 226  ASQNGYNRLLLGSCVSRIACHVISATVKGQGYSLPADVQYVDARWEIPVVLPLRDCLAKE 285

Query: 881  LTMLCHFNSWKSINISSTSILGAPNSINALVGAFVSSMQDENPSREQTIVRTAEKLKAFT 702
            LTMLC  +  K++ +  +   G    IN LV +FV+ +Q+ENPSRE TI+RTA KL  F 
Sbjct: 286  LTMLCRLDGLKTVELIRSPCTG----INGLVSSFVTLLQEENPSRECTIMRTAGKLIPFH 341

Query: 701  FNKLPE--NTAVSHLSRRRQEVLKNNTRSEASIELLCPVCSSPLNERDFDFKTSPQSIEN 528
            FNK+ E  ++ V   +RRRQ+          S E  C +C+SPL+  D     S +++E+
Sbjct: 342  FNKIEEIDDSNVLLATRRRQKRYNLKPNESFSSESFCFICNSPLSRSDL---LSLKNLES 398

Query: 527  FKELYISLSRGGKSECSPSSLFMCMCCPTCRFQIL--DDQKESNIHLLLPNLMRERAHEN 354
             K                      +CC +C+FQIL  D    + ++ LLP  +  RA  +
Sbjct: 399  HKT-------------------SSVCCSSCQFQILPQDPSSVNELYTLLPEQLFARAKHD 439

Query: 353  AFDRDSWIRKNIEDCLLSDEDGD 285
              D  S IR+ I+DCLLS+ + +
Sbjct: 440  NLDHCSAIREQIQDCLLSEGEDE 462


>XP_018841765.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein 2-like [Juglans
            regia]
          Length = 461

 Score =  334 bits (857), Expect = e-105
 Identities = 188/442 (42%), Positives = 266/442 (60%), Gaps = 8/442 (1%)
 Frame = -3

Query: 1586 CSKCSENKAQIRATNQTEFLCRDCLHANLLSKFKTSVNLNAMILPSDKVLLAFSGGPSSR 1407
            C KC  N+       +    C DC  +NL  KF+ +V  NAMI P+D VL+AFSGGPSSR
Sbjct: 42   CVKCKSNEPISGGNAEDGRFCADCFRSNLYGKFRLAVISNAMICPTDNVLVAFSGGPSSR 101

Query: 1406 VVLEFVRELRSKAQRDADASRDSILQVFDVGVAFVDESTV--AHGAQTCQLIEEIKLLLS 1233
            V L+FV E++ K+Q++ DASRD  L VF VGVAF+DE  +      +  + I++ + ++S
Sbjct: 102  VALQFVHEMQDKSQKNFDASRDRSLPVFGVGVAFIDERAIFPVPSHEIDKAIQDFRSIVS 161

Query: 1232 GNVQPKEKIHVAALECLWSENEESCKQTLLDLLASIHDTTSKEDLLGYLRMQVLQKIAIE 1053
                P++++HV  +E + S +    +  L +LL +I D+T KEDLL +LRM  LQKIA E
Sbjct: 162  ALAPPRKELHVVPIENICSSSSSDGRDRLKNLLDAISDSTGKEDLLLHLRMLSLQKIASE 221

Query: 1052 HGYSKLLLGLNASRIAVHVLSSTVKGQGYSLPADVRYIDARWEVPVVLPLRDCASKELTM 873
            +GY++++LG   S IA HV+S+ VKGQGYSLP D++Y+DARWE+PVVLPLRDC ++EL  
Sbjct: 222  NGYNRVVLGSCTSSIACHVISAIVKGQGYSLPGDIQYVDARWEIPVVLPLRDCLTQELNR 281

Query: 872  LCHFNSWKSINISSTSILGAPNSINALVGAFVSSMQDENPSREQTIVRTAEKLKAFTFNK 693
            LCH +  K++ +      G    IN LV +FV+ +Q ENPSRE TIVRTA KL  F FN 
Sbjct: 282  LCHLDGLKTVELHKVPYSG----INGLVSSFVTLLQKENPSRECTIVRTAGKLTPFHFNS 337

Query: 692  LPE----NTAVSHLSRRRQEVLKNNTRSEASIELLCPVCSSPLNERDFDFKTSPQSIENF 525
            +PE    NT ++   R ++  LK N     S E  CPVC+SPLN+ D   +++ Q+ +  
Sbjct: 338  IPEKSDSNTTLATQRRLKKYNLKPN--ESLSSESFCPVCNSPLNKSDLLRQSNLQNHQ-- 393

Query: 524  KELYISLSRGGKSECSPSSLFMCMCCPTCRFQILDDQKESNIHLL--LPNLMRERAHENA 351
                           + S +F   CC +CRFQIL     S  H    LP  +  RA  + 
Sbjct: 394  ---------------TSSDIFGAACCSSCRFQILPKDPSSMEHFYTHLPQPLVARAKHSG 438

Query: 350  FDRDSWIRKNIEDCLLSDEDGD 285
            +   S +R+ I+DCL+SD + +
Sbjct: 439  YGNLSLLREQIQDCLISDSEDE 460


>XP_019154594.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Ipomoea nil]
          Length = 475

 Score =  335 bits (858), Expect = e-105
 Identities = 194/448 (43%), Positives = 272/448 (60%), Gaps = 13/448 (2%)
 Frame = -3

Query: 1592 RSCSKCSENKAQIRA------TNQTEFLCRDCLHANLLSKFKTSVNLNAMILPSDKVLLA 1431
            R C KC  N+    +          E  C DC  +NL  KF+ SV+ NAMI PSD VL+A
Sbjct: 49   RICVKCKLNETITASGGGGGVRGDGERFCADCFRSNLYGKFRFSVSSNAMISPSDNVLVA 108

Query: 1430 FSGGPSSRVVLEFVRELRSKAQRDADASRDSILQVFDVGVAFVDESTVAHGA--QTCQLI 1257
            FSGGPSSRV L FV E++ KAQ++ +ASRD  L VF VGVAFVDE +++     +  + I
Sbjct: 109  FSGGPSSRVALHFVSEMQDKAQKNFEASRDRALPVFGVGVAFVDERSISPVPCHEFDKAI 168

Query: 1256 EEIKLLLSGNVQPKEKIHVAALECLWSENEESCKQTLLDLLASIHDTTSKEDLLGYLRMQ 1077
            EE+KL++S    P ++ HV   E ++S    + ++ L  L+ ++ DTT +EDLL + RM 
Sbjct: 169  EEMKLIVSNLAPPLKQFHVVPTESVYSLEPANGREQLKKLIDAVSDTTGREDLLEHFRML 228

Query: 1076 VLQKIAIEHGYSKLLLGLNASRIAVHVLSSTVKGQGYSLPADVRYIDARWEVPVVLPLRD 897
             LQK A+E+GY+K++LG   SRIA HVL +TVKGQGYSL AD++Y+DARWE+PV+LPLRD
Sbjct: 229  ALQKTALENGYTKIVLGTCTSRIACHVLEATVKGQGYSLAADIQYVDARWEIPVLLPLRD 288

Query: 896  CASKELTMLCHFNSWKSINISSTSILGAPNSINALVGAFVSSMQDENPSREQTIVRTAEK 717
            C + E+++LC  +S K++ + +    G  + IN LV +FV  +Q+ENPSRE TIVRTA K
Sbjct: 289  CFAHEMSLLCCLDSLKTVELFN----GLRSGINGLVSSFVKLLQEENPSRESTIVRTAGK 344

Query: 716  LKAFTFNKLPENTAVS--HLSRRRQEVLKNNTRSEASIELLCPVCSSPLNERDFDFKTSP 543
            L  F FN++PE+   +    S+RRQ+     T +    E  CP+C+SP+   D    T  
Sbjct: 345  LTPFHFNRIPEDNEYNGHTASQRRQKKYNIKTDNLIPQESFCPICNSPVKISDLTNLTRS 404

Query: 542  QSIENFKELYISLSRGGKSECSPSSLFMCMCCPTCRFQILDDQKES--NIHLLLPNLMRE 369
            + ++   +L  S                  CC +C+FQIL  +  +    H LLP+ +  
Sbjct: 405  EDVQTSIDLTGS------------------CCSSCQFQILPGELSAIDYFHSLLPDPIIA 446

Query: 368  RAHENAFDRDSWIRKNIEDCLLSD-EDG 288
            RA +  F   S +R+ IEDCLLSD EDG
Sbjct: 447  RAKDARFIHQSRLREQIEDCLLSDTEDG 474


>XP_007162194.1 hypothetical protein PHAVU_001G132100g [Phaseolus vulgaris]
            ESW34188.1 hypothetical protein PHAVU_001G132100g
            [Phaseolus vulgaris]
          Length = 458

 Score =  334 bits (856), Expect = e-105
 Identities = 190/442 (42%), Positives = 272/442 (61%), Gaps = 8/442 (1%)
 Frame = -3

Query: 1586 CSKCSENKAQ--IRATNQTEFLCRDCLHANLLSKFKTSVNLNAMILPSDKVLLAFSGGPS 1413
            C KC  N A       +   F C DC   NL  KF+ +V  NAMI P+DKVL+AFSGGPS
Sbjct: 39   CIKCKLNDAVSGYGGIDDGRF-CADCFKTNLFGKFRFAVTSNAMITPTDKVLVAFSGGPS 97

Query: 1412 SRVVLEFVRELRSKAQRDADASRDSILQVFDVGVAFVDESTVAH--GAQTCQLIEEIKLL 1239
            SRV L+FV +++ +AQR+ DASRD  L VF VGV F+DE  V     ++  + +E ++ +
Sbjct: 98   SRVALQFVHDMQERAQRNFDASRDRSLPVFGVGVVFIDEGAVLSIPSSEMEEAVEVVREV 157

Query: 1238 LSGNVQPKEKIHVAALECLWSENEESCKQTLLDLLASIHDTTSKEDLLGYLRMQVLQKIA 1059
            +S  V P++++HV  +E ++  +    K+ L+ ++ ++ D T +ED+L  LRM  LQK+A
Sbjct: 158  VSSLVPPRKELHVVPIETVYPSDSGDGKERLIKVMNTVSDPTGREDMLICLRMLALQKVA 217

Query: 1058 IEHGYSKLLLGLNASRIAVHVLSSTVKGQGYSLPADVRYIDARWEVPVVLPLRDCASKEL 879
             E GY++++LG   SRIA HV+S+TVKGQGYSLPAD++Y+DARWEVPVVLPLRDC ++E+
Sbjct: 218  SECGYNRIVLGSCISRIACHVISATVKGQGYSLPADIQYVDARWEVPVVLPLRDCFAQEI 277

Query: 878  TMLCHFNSWKSINISSTSILGAPNSINALVGAFVSSMQDENPSREQTIVRTAEKLKAFTF 699
             MLCH +  K++ +S+    G  +SIN LV +FVS +Q+ENPSRE TIVRTA KL  F F
Sbjct: 278  NMLCHLDGLKTVKLST----GPCSSINGLVSSFVSLLQEENPSRESTIVRTAGKLTPFQF 333

Query: 698  NKLPE--NTAVSHLSRRRQEVLKNNTRSEASIELLCPVCSSPLNERDFDFKTSPQSIENF 525
            N++PE  +  V   +RRRQ+     +    S E  CP+C+SPLN+ +    ++  S +N 
Sbjct: 334  NRIPEIIDGNVPLATRRRQKRYNLKSNESVSSESFCPLCNSPLNKSEIVDWSNMDSHQN- 392

Query: 524  KELYISLSRGGKSECSPSSLFMCMCCPTCRFQIL--DDQKESNIHLLLPNLMRERAHENA 351
                             S  F   CC +C+FQIL  D       ++ LP  M  RA + +
Sbjct: 393  -----------------SDNFYNTCCSSCQFQILPSDSTSMEQFYMDLPQSMVGRAKQAS 435

Query: 350  FDRDSWIRKNIEDCLLSDEDGD 285
                S +R+ I+D LLSD + +
Sbjct: 436  NGDLSLLREQIQDFLLSDGEDE 457


>XP_002529606.2 PREDICTED: cytoplasmic tRNA 2-thiolation protein 2, partial [Ricinus
            communis]
          Length = 411

 Score =  332 bits (850), Expect = e-105
 Identities = 182/416 (43%), Positives = 260/416 (62%), Gaps = 6/416 (1%)
 Frame = -3

Query: 1526 CRDCLHANLLSKFKTSVNLNAMILPSDKVLLAFSGGPSSRVVLEFVRELRSKAQRDADAS 1347
            C DC  +N+  KF+ +V  +AM+ PSD VL+AFSGGPSS V L+FV +++ +AQ++ DAS
Sbjct: 16   CADCFRSNIYGKFRVAVTSHAMMTPSDNVLVAFSGGPSSMVALQFVHDMQLRAQKNFDAS 75

Query: 1346 RDSILQVFDVGVAFVDESTVAH--GAQTCQLIEEIKLLLSGNVQPKEKIHVAALECLWSE 1173
            +D  L VF VGVAF+DE  V      +    I E+ L++S    P +++H+  +E ++S 
Sbjct: 76   KDRSLPVFGVGVAFIDERAVYQLPSEKLDDAIREMNLVVSSLAPPTKRLHIVPIENVYSP 135

Query: 1172 NEESCKQTLLDLLASIHDTTSKEDLLGYLRMQVLQKIAIEHGYSKLLLGLNASRIAVHVL 993
            +    K  L++LL  ++D T KEDLL +LRM  LQK+A E+GY++L+LG   SRIA HVL
Sbjct: 136  DSIDGKDRLMELLNHVNDATGKEDLLLHLRMLALQKVASENGYNRLVLGSCTSRIACHVL 195

Query: 992  SSTVKGQGYSLPADVRYIDARWEVPVVLPLRDCASKELTMLCHFNSWKSINISSTSILGA 813
            S+TVKGQGYSL AD++Y+D+RWEVP+VLPLRDC ++EL+++C  +  K++ + +  + G 
Sbjct: 196  SATVKGQGYSLSADIQYVDSRWEVPLVLPLRDCTAQELSLMCRIDGLKTMELLNYPLTG- 254

Query: 812  PNSINALVGAFVSSMQDENPSREQTIVRTAEKLKAFTFNKLPE--NTAVSHLSRRRQEVL 639
               I  LV +FV+ +Q+ENPSRE TIVRTA KL  F FN++PE  +  V   +RRRQ+  
Sbjct: 255  ---IYGLVSSFVTLLQEENPSRESTIVRTAGKLTPFHFNRIPEINDCNVPLATRRRQKRY 311

Query: 638  KNNTRSEASIELLCPVCSSPLNERDFDFKTSPQSIENFKELYISLSRGGKSECSPSSLFM 459
                    S EL CP+C+SPLN+ +     SP+S +                   SS F 
Sbjct: 312  NIKPVESISSELFCPICNSPLNDSEISRLNSPKSCQ-------------------SSSFR 352

Query: 458  CMCCPTCRFQIL--DDQKESNIHLLLPNLMRERAHENAFDRDSWIRKNIEDCLLSD 297
              CC +C FQIL  D     + +L LP  +  RA   + D  S +R+ I+DCLLSD
Sbjct: 353  DACCSSCNFQILPKDPSLMEHFYLSLPQHLVTRAKRGSSDNLSLLREQIQDCLLSD 408


>XP_014494738.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Vigna radiata
            var. radiata]
          Length = 458

 Score =  333 bits (854), Expect = e-105
 Identities = 189/442 (42%), Positives = 271/442 (61%), Gaps = 8/442 (1%)
 Frame = -3

Query: 1586 CSKCSENKAQ--IRATNQTEFLCRDCLHANLLSKFKTSVNLNAMILPSDKVLLAFSGGPS 1413
            C KC  N A       +   F C DC   NL  KF+ +V  NAMI P+DKVL+AFSGGPS
Sbjct: 39   CIKCKLNDAVSGYGGIDDGRF-CADCFKTNLFGKFRFAVTSNAMITPTDKVLVAFSGGPS 97

Query: 1412 SRVVLEFVRELRSKAQRDADASRDSILQVFDVGVAFVDESTVAH--GAQTCQLIEEIKLL 1239
            SRV L+FV +++ +AQR+ DASRD  L VF VGV F+DES V     ++  + +E I+ +
Sbjct: 98   SRVALQFVHDMQERAQRNFDASRDRSLPVFGVGVVFIDESAVLSIPSSEMEEAVEVIREV 157

Query: 1238 LSGNVQPKEKIHVAALECLWSENEESCKQTLLDLLASIHDTTSKEDLLGYLRMQVLQKIA 1059
            +S    P +++H+  +E ++S +    K+ L+ ++ ++ D T +ED+L  +RM  LQK+A
Sbjct: 158  VSCLASPTKELHIVPIETVYSSDSGDGKERLIKVMNTVTDPTGREDMLLCMRMLALQKVA 217

Query: 1058 IEHGYSKLLLGLNASRIAVHVLSSTVKGQGYSLPADVRYIDARWEVPVVLPLRDCASKEL 879
             E GY++++LG   SRIA HV+S+TVKGQGYSLPAD++Y+DARWEVP+VLPLRDC ++E+
Sbjct: 218  SECGYNRIVLGSCISRIACHVISATVKGQGYSLPADIQYVDARWEVPIVLPLRDCFAQEI 277

Query: 878  TMLCHFNSWKSINISSTSILGAPNSINALVGAFVSSMQDENPSREQTIVRTAEKLKAFTF 699
             MLCH +  K++  S+    G  +SIN LV +FVS +Q+ENPSRE TIVRTA KL  F F
Sbjct: 278  NMLCHLDGLKTVKFST----GPCSSINRLVSSFVSLLQEENPSRESTIVRTAGKLTPFQF 333

Query: 698  NKLPE--NTAVSHLSRRRQEVLKNNTRSEASIELLCPVCSSPLNERDFDFKTSPQSIENF 525
            N++PE  +  V   +RRRQ+     +    S E  CP+C+SPLN+ +    ++  S  N 
Sbjct: 334  NRIPEIIDGNVPLATRRRQKRYNLKSNESVSSESFCPLCNSPLNKSEIVDWSNMDSHRN- 392

Query: 524  KELYISLSRGGKSECSPSSLFMCMCCPTCRFQIL--DDQKESNIHLLLPNLMRERAHENA 351
                             S  F   CC +C+FQIL  D       ++ LP  M  RA + +
Sbjct: 393  -----------------SDSFYNTCCSSCQFQILPSDSTLIEQFYMDLPQPMVSRAKQAS 435

Query: 350  FDRDSWIRKNIEDCLLSDEDGD 285
            +   S +R+ I+D LLSD + +
Sbjct: 436  YGNLSLLREQIQDFLLSDGEDE 457


>XP_019444040.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Lupinus
            angustifolius]
          Length = 450

 Score =  333 bits (853), Expect = e-105
 Identities = 186/438 (42%), Positives = 265/438 (60%), Gaps = 8/438 (1%)
 Frame = -3

Query: 1586 CSKCSENKAQ--IRATNQTEFLCRDCLHANLLSKFKTSVNLNAMILPSDKVLLAFSGGPS 1413
            C KC  N A       +   F C DC  +NL  KF+ +V  NAMI P+DKVL+AFSGGPS
Sbjct: 33   CIKCKLNDAVSGYGGVDDGRF-CADCFRSNLFGKFRLAVTSNAMITPTDKVLVAFSGGPS 91

Query: 1412 SRVVLEFVRELRSKAQRDADASRDSILQVFDVGVAFVDESTVAH--GAQTCQLIEEIKLL 1239
            SRV L+FV +++ +AQ++ DASRD  L VF VGV F+DES +      +  + +E I  +
Sbjct: 92   SRVALQFVHDMQERAQKNFDASRDRSLPVFGVGVVFIDESAILSIPSGEMEEAVEVISSV 151

Query: 1238 LSGNVQPKEKIHVAALECLWSENEESCKQTLLDLLASIHDTTSKEDLLGYLRMQVLQKIA 1059
            +S    P +++H+  +E ++S +    K+ L+ L+ S+ D T +ED+L YLRM  LQK+A
Sbjct: 152  VSSLAPPTKELHIVPIENVYSSDSSDGKEKLIKLVGSVSDPTGREDMLLYLRMLALQKVA 211

Query: 1058 IEHGYSKLLLGLNASRIAVHVLSSTVKGQGYSLPADVRYIDARWEVPVVLPLRDCASKEL 879
             E GY++L+LG   SRIA HV+S+TVKGQGYSLPAD++Y+DARWE+P+VLPLRDC ++E+
Sbjct: 212  SEFGYNRLVLGSCVSRIASHVISATVKGQGYSLPADIQYVDARWEIPIVLPLRDCFAQEI 271

Query: 878  TMLCHFNSWKSINISSTSILGAPNSINALVGAFVSSMQDENPSREQTIVRTAEKLKAFTF 699
             M CH +  K+  +S+    G  ++IN+LV +FV+ +Q+ENPSRE TIVRTA KL  F F
Sbjct: 272  NMFCHLDGLKTFRLST----GPCSTINSLVSSFVALLQEENPSRESTIVRTAGKLIPFQF 327

Query: 698  NKLPE--NTAVSHLSRRRQEVLKNNTRSEASIELLCPVCSSPLNERDFDFKTSPQSIENF 525
            N++PE  +  V   +RRRQ+          S E  C +C+SPL++ +             
Sbjct: 328  NRIPEIVDGKVPLATRRRQKRYNLKPNDSVSSESFCLLCNSPLDKSEI------------ 375

Query: 524  KELYISLSRGGKSECSPSSLFMCMCCPTCRFQIL--DDQKESNIHLLLPNLMRERAHENA 351
                +  S    S    S +F   CC +C FQIL  D       ++ LP  +  RA +  
Sbjct: 376  ----VDASNADNSR--SSDIFYSACCSSCHFQILPSDSISMEQFYMDLPQSVLSRAKQAN 429

Query: 350  FDRDSWIRKNIEDCLLSD 297
                S +R+ I+DCLLSD
Sbjct: 430  NGNLSLLREQIQDCLLSD 447


>XP_016543722.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Capsicum annuum]
          Length = 480

 Score =  333 bits (855), Expect = e-104
 Identities = 198/450 (44%), Positives = 267/450 (59%), Gaps = 17/450 (3%)
 Frame = -3

Query: 1586 CSKCSENKAQIRATNQTEFL----------CRDCLHANLLSKFKTSVNLNAMILPSDKVL 1437
            C KC  N     +T     +          C DC  +NL  KF+ +V  NAMI PSD VL
Sbjct: 52   CIKCKVNTTIAASTADVSVVDGGGSDAGRFCVDCFRSNLFGKFRLAVTANAMISPSDNVL 111

Query: 1436 LAFSGGPSSRVVLEFVRELRSKAQRDADASRDSILQVFDVGVAFVDESTVA--HGAQTCQ 1263
            +AFSGG  SRV L+F+ E+++KAQ++ DASRD  L VF VGVAF+DE T +        +
Sbjct: 112  VAFSGGTCSRVALQFIHEMQTKAQKNFDASRDRALPVFGVGVAFIDEKTCSAVSSGYLDR 171

Query: 1262 LIEEIKLLLSGNVQPKEKIHVAALECLWSENEESCKQTLLDLLASIHDTTSKEDLLGYLR 1083
             IEE+KL++S    P ++ HV   E ++S  E   K  L +L+ ++ D T KEDLL + R
Sbjct: 172  AIEEMKLIVSDLAPPVKQFHVVPTETIYSP-EAGAKDRLNELINAVSDITGKEDLLEHFR 230

Query: 1082 MQVLQKIAIEHGYSKLLLGLNASRIAVHVLSSTVKGQGYSLPADVRYIDARWEVPVVLPL 903
            M  LQKIAIEHGY+K+LLG   SRIA HVL +TVKG+GYSL AD++Y+DARW++PVVLPL
Sbjct: 231  MLSLQKIAIEHGYTKVLLGTCTSRIACHVLEATVKGRGYSLAADIQYVDARWKIPVVLPL 290

Query: 902  RDCASKELTMLCHFNSWKSINISSTSILGAPNSINALVGAFVSSMQDENPSREQTIVRTA 723
            RDC   EL MLC+ +S  ++ + +    G    IN L+ +FV  +Q+ENPSRE TI+RTA
Sbjct: 291  RDCPIHELNMLCNLDSLMTVEVFN----GTRAGINGLISSFVKLLQEENPSRESTIMRTA 346

Query: 722  EKLKAFTFNKLPENTAVSH--LSRRRQEVLKNNTRSEASIELLCPVCSSPLNERDFDFKT 549
             KL  F FN++PE+   +    SRRRQ+     T+    IE  CP+C+SPL   +F    
Sbjct: 347  GKLTPFHFNRMPEDNDYNGQVASRRRQKKYNLKTKDALLIESFCPICNSPLKVSNF---- 402

Query: 548  SPQSIENFKELYISLSRGGKSECSPSSLFMCMCCPTCRFQIL--DDQKESNIHLLLPNLM 375
              ++  NF          GK+  SP+ +    CC +C+FQ+L  D     N   LLP  M
Sbjct: 403  --ETSSNFD--------NGKT--SPNGI-GAACCASCQFQVLPKDPSSLENFISLLPPSM 449

Query: 374  RERAHENAFDRDSWIRKNIEDCLLSD-EDG 288
              +A +        +R+ IEDCLLSD EDG
Sbjct: 450  VSQAEDGVRLSQRQLREQIEDCLLSDNEDG 479


>JAT44977.1 Cytoplasmic tRNA 2-thiolation protein 2, partial [Anthurium amnicola]
          Length = 484

 Score =  333 bits (855), Expect = e-104
 Identities = 194/439 (44%), Positives = 266/439 (60%), Gaps = 5/439 (1%)
 Frame = -3

Query: 1586 CSKCSENKAQIRATNQTEFLCRDCLHANLLSKFKTSVNLNAMILPSDKVLLAFSGGPSSR 1407
            CSKC E+ A          LC  C  + L  KFK +V  NAMI P+D VL+A SGGP+SR
Sbjct: 69   CSKCREHPAYCNN------LCGGCFRSALFGKFKLAVTSNAMISPTDNVLVALSGGPASR 122

Query: 1406 VVLEFVRELRSKAQRDADASRDSILQVFDVGVAFVDESTVAHGAQTCQ-LIEEIKLLLSG 1230
            V L+FV E++ K+Q+  DAS+D  L VF VGVAF+DE+     ++  +  I+EI+L +S 
Sbjct: 123  VALQFVHEMQLKSQKSWDASKDQSLPVFGVGVAFIDETMFLRSSKEVEKAIDEIRLCVSQ 182

Query: 1229 NVQPKEKIHVAALECLWSENEESCKQTLLDLLASIHDTTSKEDLLGYLRMQVLQKIAIEH 1050
               PK+ +HVA++  ++S + +     L D+L +I D T KEDLL +LR+  LQKIA E+
Sbjct: 183  LAPPKKVLHVASIGSIFSADPKDGTARLNDVLETISDATGKEDLLHHLRILSLQKIASEN 242

Query: 1049 GYSKLLLGLNASRIAVHVLSSTVKGQGYSLPADVRYIDARWEVPVVLPLRDCASKELTML 870
            GYSKL+LG   + IA HVLS+TVKGQGYSLPADV+Y+DARW VPVV PLRDC S+ELTML
Sbjct: 243  GYSKLMLGSCTTTIACHVLSATVKGQGYSLPADVQYVDARWTVPVVQPLRDCLSEELTML 302

Query: 869  CHFNSWKSINISSTSILGAPNS-INALVGAFVSSMQDENPSREQTIVRTAEKLKAFTFNK 693
            CH +S K     +  +L  P++ IN LV +FV+ +Q+ENPSR +TI+RTAEKL+ F FNK
Sbjct: 303  CHLDSLK-----TQEVLDQPHAGINNLVSSFVARVQEENPSRARTIMRTAEKLRPFYFNK 357

Query: 692  LPENTAVSHLSRRRQEVLKNNTRSEASI-ELLCPVCSSPLNERDFDFKTSPQSIENFKEL 516
              EN +   L  R +  L+    +E++  E+LC +C  PL E D                
Sbjct: 358  FMENLSYDMLPSRVRRKLQTERNAESTASEVLCAICGGPLTESD---------------- 401

Query: 515  YISLSRGGKSECSPSSLFMCMCCPTCRFQILDDQKESNIHL--LLPNLMRERAHENAFDR 342
             +SL        +   +F   CC +C  QIL  +  S  H   LLP  +  R  +     
Sbjct: 402  VVSLKASLGKPSTRHEVFAAHCCQSCCTQILPKEASSLEHFYSLLPPAVTGRVKDVMCAD 461

Query: 341  DSWIRKNIEDCLLSDEDGD 285
             +W+R+ IEDCL+S ++ D
Sbjct: 462  YTWLREQIEDCLISSDEDD 480


>XP_004249262.1 PREDICTED: cytoplasmic tRNA 2-thiolation protein 2 [Solanum
            lycopersicum]
          Length = 478

 Score =  333 bits (854), Expect = e-104
 Identities = 195/447 (43%), Positives = 267/447 (59%), Gaps = 14/447 (3%)
 Frame = -3

Query: 1586 CSKCSENKAQIRATNQTEF-------LCRDCLHANLLSKFKTSVNLNAMILPSDKVLLAF 1428
            C KC  N+    A    +F        C DC  +NL  KF+ +V  NAMI PSD VL+AF
Sbjct: 52   CLKCKVNRTIAAADGGGDFGGGDAGRFCADCFRSNLFGKFRLAVTANAMISPSDNVLVAF 111

Query: 1427 SGGPSSRVVLEFVRELRSKAQRDADASRDSILQVFDVGVAFVDESTVA--HGAQTCQLIE 1254
            SGG  SRV L+F  E++SKAQ++ DASRD  L VF VGVAF+DE  ++        + +E
Sbjct: 112  SGGTCSRVALQFTHEMQSKAQKNYDASRDRALPVFGVGVAFIDEKAISAVSSDNLDRAVE 171

Query: 1253 EIKLLLSGNVQPKEKIHVAALECLWSENEESCKQTLLDLLASIHDTTSKEDLLGYLRMQV 1074
            E++L++S    P ++ HV   E ++S +    K  L +L+ +++D T KEDLL + RM  
Sbjct: 172  EMELIVSDLAPPVKQFHVVPTEAIYSLDAGDSKDRLNELINTVNDVTGKEDLLEHFRMLS 231

Query: 1073 LQKIAIEHGYSKLLLGLNASRIAVHVLSSTVKGQGYSLPADVRYIDARWEVPVVLPLRDC 894
            LQKIAIE+GY+K+LLG   SRIA HVL +TVKG+GYSL AD++Y+DARW++PVVLPLRDC
Sbjct: 232  LQKIAIENGYTKILLGTCTSRIACHVLEATVKGRGYSLAADIQYVDARWKIPVVLPLRDC 291

Query: 893  ASKELTMLCHFNSWKSINISSTSILGAPNSINALVGAFVSSMQDENPSREQTIVRTAEKL 714
               EL MLC  +S K++ + +    G    IN L+ +FV  +Q+ENPSRE TI+RTA KL
Sbjct: 292  PIHELNMLCSLDSLKTVEVFN----GTRAGINGLISSFVKLLQEENPSRESTIMRTAGKL 347

Query: 713  KAFTFNKLPENTAVSH--LSRRRQEVLKNNTRSEASIELLCPVCSSPLNERDFDFKTSPQ 540
              F FN++PE+   +    SRRRQ+     T+     E  CP+C+SPL    F       
Sbjct: 348  TPFHFNRMPEDNDYNGQVASRRRQKKYNLKTKDALPPESFCPICNSPLKISSFGTSI--- 404

Query: 539  SIENFKELYISLSRGGKSECSPSSLFMCMCCPTCRFQILDDQKESNIHL--LLPNLMRER 366
               NF+         GK+  +P+ +    CC +C+FQ+L +   S  H   LLP  M  +
Sbjct: 405  ---NFE--------NGKT--NPNGI-GAACCASCQFQVLPNDPSSLEHFISLLPPSMVSQ 450

Query: 365  AHENAFDRDSWIRKNIEDCLLSD-EDG 288
            A +N       +R+ IEDCLLSD EDG
Sbjct: 451  AGDNDRLSQRQLREQIEDCLLSDNEDG 477